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1.
Public Health ; 194: 208-215, 2021 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-33962098

RESUMO

OBJECTIVES: Diurnal temperature range (DTR) is an important indicator of global climate change. Many epidemiological studies have reported the associations between high DTR and human health. This study investigated the association between DTR and hospitalisations for ischaemic stroke in Hefei, China. STUDY DESIGN: This is an ecological study. METHODS: Data of daily hospital admissions for ischaemic stroke and meteorological variables from 1 January 2009 to 31 December 2017 were collected in Hefei, China. A generalised additive model combined with distributed lag non-linear model was used to quantify the effects of DTR on ischaemic stroke. The interactive effect between DTR and temperature was explored with a non-parametric bivariate response surface model. RESULTS: High DTR was associated with hospitalisations for ischaemic stroke. The adverse effect of extremely high DTR (99th percentile [17.1 °C]) occurred after 8 days (relative risk [RR] = 1.021, 95% confidence interval [CI] = 1.002, 1.041) and the maximum effect appeared after 12 days (RR = 1.029, 95% CI = 1.011, 1.046). The overall trend of the effect of DTR on ischaemic stroke was decreasing. In addition, there was a significant interactive effect of high DTR and low temperature on ischaemic stroke. CONCLUSIONS: This study suggests that the impact of high DTR should be considered when formulating targeted measures to prevent ischaemic stroke, especially for those days with high DTR and low mean temperature.

2.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(4): 504-513, 2021 Apr 20.
Artigo em Chinês | MEDLINE | ID: mdl-33963708

RESUMO

OBJECTIVE: To explore the synergistic inhibitory effect of polysaccharide from Trichoderma pseudokoningii (EPS) and oxaliplatin (Oxa) on colorectal cancer (CRC) HCT116 cells. OBJECTIVE: HCT116 cells were treated with 8 µg/mL Oxa and 100 µg/mL EPS alone or in combination, and the changes in cell viability was assessed with CCK-8 assay. CompuSyn software was used for fitting the Fa-CI curve to evaluate the combined effect of the two agents. Flow cytometry was performed to analyze cell apoptosis and cell cycle changes, and wound healing assay and Transwell assay were used to examine the migration ability of the treated cells. Oxa- and EPS-related genes and CRC-related genes were intersected for protein-protein interaction (PPI) analysis and GO and KEGG enrichment analyses. OBJECTIVE: Treatment with Oxa alone or in combination with EPS significantly inhibited the viability of HCT116 cells in a dose- and time-dependent manner, and the two agents exhibited a significant synergistic effect (CI < 1). The combined treatment with Oxa and EPS resulted in a significantly higher total cell apoptosis rate and a higher percentage of cells in S phase than Oxa alone and the control treatment (P < 0.05). EPS and Oxa alone both inhibited the migration of HCT116 cells, and their combination produced a stronger inhibitory effect. GO enrichment analysis of the key genes related with Oxa, EPS and CRC suggested that these genes were involved mainly in such biological processes as exogenous apoptosis signaling, cell response to chemical stress, and reactive oxygen metabolism; KEGG analysis showed that these genes were involved in the pathways of drug resistance, apoptosis and angiogenesis. OBJECTIVE: EPS and Oxa can synergistically inhibit the proliferation of HCT116 cells possibly through the PI3K-Akt, MAPK, VEGF, and p53 signaling pathways.

3.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(4): 621-627, 2021 Apr 20.
Artigo em Chinês | MEDLINE | ID: mdl-33963725

RESUMO

OBJECTIVE: To explore the factors affecting the survival of patients with advanced gastric cancer and establish a reliable predictive model of the patients' survival outcomes. OBJECTIVE: We retrospectively collected the clinical data from patients with advanced gastric cancer treated in our department between January, 2015 and December, 2019. Univariate survival analysis was carried out using Kaplan-Meier method followed by multivariate Cox regression analysis to identify the factors associated with the survival outcomes of the patients. The R package was used to generate the survival rates, and a nomogram was established based on the results of multivariate analysis. The calibration curves and C-index were calculated to determine the predictive and discriminatory power of the model. The performance of the nomogram model for predicting the survival outcomes of the patients was evaluated using receiver- operating characteristic (ROC) curve analysis and decision curve analysis (DCA). OBJECTIVE: Univariate analysis showed that the number of metastatic sites, the number of treatment lines received, disease control rate (DCR) and progression-free survival (PFS) time following first-line treatment, and surgical treatment in first-line treatment were significantly correlated with the survival time of the patients (P < 0.05). Multivariate Cox regression analysis showed that surgical treatment, number of treatment lines, PFS time following first-line treatment and peritoneal metastasis, as independent prognostic factors, were significantly correlated with the patients' survival (P < 0.05). The C-index of the nomogram was 0.785 (95%CI: 0.744-0.826) for overall survival of the patients. The calibration curves showed that the actual survival rate of the patients was consistent with the predicted survival rate. The time-dependent AUC and DCA demonstrated that the nomogram had a good performance for predicting the survival outcomes of patients with advanced gastric cancer. OBJECTIVE: Peritoneal metastasis is associated with s shorter overall survival time of patients with advanced gastric cancer, while a PFS time following first-line treatment of more than 7.0 months and third-line and posterior-line treatments are related with a longer survival time. Systematic treatment including elective surgery can improve the survival outcomes of the patients. The nomogram we established provides a reliable prognostic model for evaluating the prognosis of patients with advanced gastric cancer.

4.
Med Vet Entomol ; 2021 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-33942346

RESUMO

Filth flies are of medical and veterinary importance because of the transfer of disease organisms to animals and humans. The traditional control methods include the use of chemical insecticides. A novel mechanical insecticide made from volcanic glass and originally developed to control mosquitoes (Imergard™ WP; ImG) was investigated for control of adult grey flesh flies, Sarcophaga bullata (Parker), secondary screwworms, Cochliomyia macellaria (F.), and house flies, Musca domestica L. In a modified WHO cone test device, the time to 50% mortality (LT50 ) when applied at 5 g/m2 (tested at 30 °C and 50% relative humidity (rH)) was 7.1, 4.3 and 3.2 h, respectively. When knockdown was included, the LT50 s were 5.5, 1.5 and 2.8 h, respectively. Application rates of 1.25 and greater g/m2 had the shortest LT50 s. The time to the LT50 increased for M. domestica as rH increased, but ImG was still active at the highest rH tested of 70%. Scanning electron micrographs showed ImG was present on all body parts, unlike that for mosquitoes where it was found mostly on the lower legs. These first studies on the use of Imergard WP against flies suggest this could be an alternative method for filth fly control.

5.
Artigo em Inglês | MEDLINE | ID: mdl-33915295

RESUMO

OBJECTIVE: To delineate the activities of decorin and biglycan in the progression of post-traumatic osteoarthritis (PTOA). DESIGN: Three-month-old inducible biglycan (BgniKO) and decorin/biglycan compound (Dcn/BgniKO) knockout mice were subjected to the destabilization of the medial meniscus (DMM) surgery to induce PTOA. The OA phenotype was evaluated by assessing joint structure and sulfated glycosaminoglycan (sGAG) staining via histology, surface collagen fibril nanostructure and calcium content via scanning electron microscopy, tissue modulus via atomic force microscopy-nanoindentation, as well as subchondral bone structure and meniscus ossification via micro-computed tomography. Outcomes were compared with previous findings in the inducible decorin (DcniKO) knockout mice. RESULTS: In the DMM model, BgniKO mice developed similar degree of OA as the control (0.44 [-0.18 1.05] difference in modified Mankin score), different from the more severe OA phenotype observed in DcniKO mice (1.38 [0.91 1.85] difference). Dcn/BgniKO mice exhibited similar histological OA phenotype as DcniKO mice (1.51 [0.97 2.04] difference versus control), including aggravated loss of sGAGs, salient surface fibrillation and formation of osteophyte. Meanwhile, Dcn/BgniKO mice showed further cartilage thinning than DcniKO mice, resulting in the exposure of underlying calcified tissues and aberrantly high surface modulus. BgniKO and Dcn/BgniKO mice developed altered subchondral trabecular bone structure in both Sham and DMM groups, while DcniKO and control mice did not. CONCLUSION: In PTOA, decorin plays a more crucial role than biglycan in regulating cartilage degeneration, while biglycan is more important in regulating subchondral bone structure. The two have distinct activities and modest synergy in the pathogenesis of PTOA.

6.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(3): 418-423, 2021 Mar 25.
Artigo em Chinês | MEDLINE | ID: mdl-33849834

RESUMO

OBJECTIVE: To explore the role of prostacyclin (PGI2) and thromboxane A2 (TXA2) in lung hyper-permeability induced by mechanical ventilation (MV) in rabbits. OBJECTIVE: Forty-eight healthy Japanese white rabbits were randomly allocated to vehicle treatment group (group V), tranylcypromine (a PGI2 synthase inhibitor) treatment group (group T), dazoxiben (a TXA2 synthase inhibitor) treatment group (group D), vehicle-treated MV group (group VM), tranylcyprominetreated MV group (group TM) and dazoxiben-treated MV group (group DM). The contents of PGI2 and TXA2 in the lung tissues and TNF-α level in BALF and lung tissues were measured by ELISA. The lung wet/dry weight (W/D) ratio, lung permeability index and pulmonary expressions of myosin light chain kinase (MLCK) protein and mRNA were detected to evaluate the pulmonary permeability. The severities of lung injury were assessed by lung histological scores. OBJECTIVE: The measured parameters did not differ significantly among the rabbits receiving different treatments without MV. In rabbits in group VM, the contents of PGI2 and TXA2 in the lungs, TNF-α in BALF and lung tissues, PGI2/TXA2 ratio, lung W/D ratio, lung permeability index, pulmonary expressions of MLCK protein and mRNA and histological scores of the lungs all increased significantly (P < 0.05) as compared with those in group V, group T and group D. In rabbits undergoing MV, inhibition of PGI2 production by tranylcypromine significantly decreased the PGI2/TXA2 ratio (P < 0.05), further enhanced the production of TNF-α in the BALF and lung tissue (P < 0.05), and worsened lung hyper-permeability and lung injury (P < 0.05), while treatment with dazoxiben significantly reduced TXA2 production in the lung tissue (P < 0.05), increased the PGI2/TXA2 ratio (P < 0.05) and decreased TNF-α production in the BALF and lung tissue (P < 0.05), thus resulting in alleviated lung hyperpermeability and lung injury (P < 0.05). OBJECTIVE: PGI2 plays a protective role against MV-induced lung hyper-permeability and lung injury by downregulating TNF-α/MLCK signaling pathway, while TXA2 can exacerbate MV-induced lung hyperpermeability in rabbits by up-regulating TNF-α/ MLCK signaling pathway.


Assuntos
Epoprostenol , Tromboxano A2 , Animais , Pulmão , Permeabilidade , Coelhos , Respiração Artificial/efeitos adversos
7.
Zhonghua Gan Zang Bing Za Zhi ; 29(3): 284-288, 2021 Mar 20.
Artigo em Chinês | MEDLINE | ID: mdl-33902199

RESUMO

Patients in "immunotolerant phase" of chronic hepatitis B virus infection are HBsAg and HBeAg-positive, with high HBV DNA level, normal ALT and no obvious histopathological hepatic necrotizing inflammation or fibrosis. However, in recent years, some studies have found that HBV DNA integration, clonal hepatocyte expansion, HBV-specific T cell immune response, liver injury and disease progression exist in patients with "immunotolerant phase" of chronic HBV infection. Therefore, the concept of "immunotolerant phase" is controversial. This paper summarizes the new insights into the "immunotolerant phase" of chronic hepatitis B virus infection, including its new concepts in nomenclature, diagnosis, treatment and management.


Assuntos
Hepatite B Crônica , Neoplasias Hepáticas , DNA Viral , Antígenos de Superfície da Hepatite B , Antígenos E da Hepatite B , Vírus da Hepatite B/genética , Humanos , Tolerância Imunológica
8.
Clin Radiol ; 2021 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-33902887

RESUMO

AIM: To explore the magnetic resonance imaging (MRI) differences between pancreatic neuroendocrine tumour grade 3 (pNET-G3) and pancreatic neuroendocrine carcinoma grade 3 (pNEC-G3). MATERIALS AND METHODS: Between 2009 and 2019, 31 patients underwent pNEN-G3 resection with preoperative MRI in two local hospitals in China. The 31 patients were assigned to a pNET-G3 group (n=13) or a pNEC-G3 group (n=18). The MRI findings between the groups were compared. RESULTS: There was no statistically significant difference between the two groups in lesion size, clinical characteristics, or laboratory indexes. The lesions showed high or slightly higher signal on diffusion-weighted imaging and decreased apparent diffusion coefficient (ADC) values, which differed between the two groups (p=0.013). The difference between the groups regarding positive enhancement integral, arterial phase and portal phase signal enhancement ratio were statistically significant; however, the delayed phase signal enhancement ratio was not significantly different. CONCLUSIONS: pNET-G3 and pNEC-G3 showed different characteristics on MRI. In particular, the ADC value and dynamic enhanced imaging could have an important role in distinguishing between the two.

9.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(5): 452-457, 2021 May 09.
Artigo em Chinês | MEDLINE | ID: mdl-33904280

RESUMO

Objective: To evaluate the preliminary clinical effect of flap-raising and cortical-perforation based extraction method in patients with potential risk of medication-related osteonecrosis of the jaw (MRONJ). Methods: Eighteen patients, who needed teeth extraction in the Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology from February 2016 to November 2020, with a history of using anti-resorption/anti-angiogenesis medication were included in this retrospective study. According to the characteristics of the patient's medication history, patients were stratified into three categories, low-dose anti-resorption drug group, high-dose anti-resorption drug group, and high-dose anti-resorption combined with anti-angiogenesis targeted drug group. There were 15 females and 3 males, the average age was 62.4 years (range from 27 to 87 years) old. A total of 31 teeth were indicated for extraction due to chronic infection. The flap-raising and cortical-perforation techniques were used to extract the affected teeth, and the patients were followed up closely. By observing the healing status and swelling degree of the mucosa of tooth extraction sites, whether there was a fistula, pus and bone exposure of jaw bone,the healing of the tooth extraction sites were evaluated. Results: Among the 18 patients, there were 9 cases of osteoporosis and 9 cases of malignant tumors. Classified by medication-using history, 10 cases were treated with low-dose anti-resorption drugs, 5 cases were high-dose anti-resorption drugs, and 3 cases were high-dose anti-resorption drugs combined with anti-angiogenesis drugs. A total of 31 teeth of the patients were extracted by flap-raising and cortical-perforation based extraction method. Thirteen patients completed treatment underwent local anesthesia and five cases were performed under general anesthesia. The shortest follow-up period was 3 months with an average of 13.2 months. Seventeen patients recovered well after the tooth extraction. One patient had the mandible exposed at one extraction site one month after the surgery, resulting in MRONJ. Conclusions: In patients with potential risks of MRONJ, the application of flap-raising and cortical-perforation based teeth extraction method could safely and effectively alleviate the dental inflammation in the oral cavity.

10.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(5): 497-501, 2021 May 09.
Artigo em Chinês | MEDLINE | ID: mdl-33904287

RESUMO

For decades, the molecular and cellular mechanisms that govern tooth development have been extensively investigated. However, most of the studies are based on mice, whose teeth are quite different from human teeth in morphological and developmental aspects. Mice are not the ideal model for understanding the development of permanent teeth as they have only one set of dentition. Thus, using of diphyodont mammals is a better model to study the deciduous and permanent tooth development and to understand the process of tooth replacement. Several diphyodont mammal models have been established including minipig, ferret, house shrew and rabbit. Studies based on the diphyodont mammals have characterized the morphological changes involved in tooth replacement and molecular mechanisms of tooth replacement. However, few developmental stages were studied on ferret due to the presence of seasonal estrus and the difficulty to obtain ferret embryos at the correct stage. The house shrew is limited as a model because their deciduous tooth germs become vestigial in the embryonic period. The main disadvantage of the rabbit is an incomplete dentition with the lack of canines. Compared to the above mentioned animal models, the miniature pig has proven to be a valuable animal model for diphyodont development due to its dentition similarities, including the morphology, number and size of teeth, to human's, and particularly its heterodont dentition consisting of incisors, canines, premolars and molars. The present article reviews the current knowledge on the development of the primary and successional teeth in minipig modle and briefly summarizes the studies based on other diphyodont mammal models.

11.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(5): 502-506, 2021 May 09.
Artigo em Chinês | MEDLINE | ID: mdl-33904288
12.
J Endocrinol Invest ; 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33829394

RESUMO

AIMS: To evaluate the associations of morning serum cortisol levels with obesity defined by different indices in Chinese rural populations. MATERIALS AND METHODS: A cross-sectional study was performed including 6198 participants (2566 males and 3632 females). Serum cortisol was collected in morning and quantified by liquid chromatography-tandem mass spectrometry. Obesity was defined by body mass index (BMI), body fat percentage (BFP), waist-to-height ratio (WHtR), waist circumference (WC), visceral fat index (VFI) and waist-to-hip ratio (WHR). Both multivariable liner regression, logistic regression and restrictive cubic splines models were used to estimate the gender-specific relationships between cortisol levels and obesity defined by different indices, respectively. RESULTS: After adjusting for potential confounders, serum cortisol was negatively associated with different obesity measures, except obese females defined by BFP (for instance, overall obesity defined by BMI, Quartile 4 vs. Quartile 1, odds ratio (OR) = 0.25, 95% confidence interval (CI):0.15, 0.41 in males, and OR = 0.58, 95% CI: 0.42,0.80 in females, central obesity defined by WC, OR = 0.52, 95% CI:0.39,0.69 in males and OR = 0.63, 95% CI:0.51,0.77 in females). Similarly, restrictive cubic splines showed the nonlinear relationship between high levels of cortisol and different obesity indices. Furthermore, ROC curve analysis indicated that cortisol could improve the discrimination of model with common biomarkers. CONCLUSION: Morning serum cortisol were negatively related to obesity defined by different indices in Chinese rural populations. In addition, cortisol could be as a biomarker for prediction of obesity in males.

13.
Eur Rev Med Pharmacol Sci ; 25(6): 2554-2566, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33829442

RESUMO

OBJECTIVE: This present study aimed to compare the treatment response, survival profile, quality of life (QoL), and safety between drug-eluting bead bronchial arterial chemoembolization (DEB-BACE) and chemotherapy in the treatment of advanced non-small-cell lung cancer (NSCLC). PATIENTS AND METHODS: Totally, 44 advanced NSCLC patients were analyzed retrospectively and were divided into DEB-BACE group (n=23) and chemotherapy group (n=21). Treatment response, European Organization for Research and Treatment of Cancer QoL Questionnaire-Core 30 (EORTC QLQ-C30), progression-free survival (PFS), overall survival (OS), and adverse events were assessed during the follow-up. RESULTS: At month (M) 2, M4 and M6 post initial treatment, objective response rate (ORR) was elevated (all p <0.05), and disease control rate (DCR) tended to be higher (without statistical significance) in DEB-BACE group compared with chemotherapy group. Regarding the QLQ-C30 item scores, the scores of physical functioning, role functioning, emotional functioning, cognitive functioning, social functioning were increased, while the scores of nausea and vomiting, dyspnea, constipation were decreased in DEB-BACE group compared with chemotherapy group (all p <0.05). Based on survival profile, DEB-BACE group achieved better PFS and OS compared with chemotherapy group independent of TNM stage, which was also supported by further subgroup analysis and Cox's proportional hazard regression analysis (all p <0.05). Furthermore, two groups all exhibited mild and tolerable adverse events. CONCLUSIONS: DEB-BACE has the potential to be an additional treatment option with favorable therapeutic efficacy, improved QoL, and tolerable safety for advanced NSCLC patients.

16.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(4): 335-341, 2021 Apr 09.
Artigo em Chinês | MEDLINE | ID: mdl-33832034

RESUMO

Objective: To study the effect of various concentrations of Enterococcus faecalis (Ef) supernatants on human periodontal ligament cell (hPDLC) and the inflammatory response of hPDLC under static pressure. Methods: The method of methyl thiazolyl tetrazolium (MTT) was used to detect the effect of various concentrations of Ef supernatants on the proliferation of hPDLCs and the flow cytometry was used to detect the expression of Toll-like receptor 2 (TLR-2) on the surface of hPDLC after 24-hour-stimulation of Ef supernatant. Furthermore, the hPDLCs were divided into non inducing group without Ef supernatant and inducing group with 5% Ef supernatant, and hPDLCs in each group were loaded with 0, 49 and 196 Pa static pressures respectively. The expressions of tumor necrosis factor-α (TNF-α) and interleukin-1ß (IL-1ß) mRNA and protein were detected by reverse transcription-PCR (RT-PCR) and enzyme linked immunosorbent assay (ELISA) after 24 hours. Results: MTT results showed that the supernatant of Ef with concentration≥5% could significantly inhibit the proliferation activity of hPDLCs at 48 hours of cell culture (P<0.05). Flow cytometry showed that the positive cell rates of TLR-2 increased with increasing volume fractions of the Ef supernatants. The values were (2.12±0.07)%, (2.41±0.32)%, (2.65±0.27)%, (4.76±0.46)%, (9.91±0.92)% and (12.01±1.35)%, respectively. The differences were statistically significant when the concentrations≥5% (P<0.05). There were no significant differences in the expressions of IL-1ß and TNF-α mRNA between the non inducing group and the control group under the pressure of 49 Pa (P>0.05). However, there were significant differences in the expressions of IL-1ß and TNF-α mRNA between the non inducing group and the control group under the pressure of 196 Pa (P<0.05), while the expressions of IL-1ß and TNF-α in the inducing group were significantly lower than that in the control group under the pressures of 49 and 196 Pa (P<0.05). Compared with the control group, the mRNA expression was significantly increased (P<0.05). The result of ELISA was consistent with that of PCR. Conclusions: High concentration of Ef supernatant could inhibit the proliferation of hPDLC. Ef supernatant might promote the expression of TLR-2 on the surface of hPDLC. Excessive mechanical pressure induced the inflammatory response of hPDLC. The presence of inflammatory mediators could lead to the intolerance of hPDLC to pressures and small pressure could aggravate the inflammatory response.


Assuntos
Enterococcus faecalis , Ligamento Periodontal , Humanos , Interleucina-1beta , Lipopolissacarídeos , RNA Mensageiro , Fator de Necrose Tumoral alfa
17.
Artigo em Chinês | MEDLINE | ID: mdl-33910281

RESUMO

Objective: To study the effects of combined occupational exposure of benzene, toluene, and xylene on human metabolism at an overall level, and to screen biomarkers related to the combined occupational exposure of benzene, toluene, and xylene, and to explore the mechanism of early health effects preliminarily caused by combined occupational exposure of benzene, toluene, and xylene by identification of biomarkers and retrieval of metabolic pathways. Methods: A shoe-making company was selected as the research site. Twenty subjects for the exposed group and the control group were selected separately, and urine of the subjects was collected. The metabolic profiles of the samples were collected by liquid chromatography time-of-flight mass spectrometry, and professional metabolomics and multivariate statistical analysis software were used to establish PCA and OPLS-DA analysis models to screen potential biomarkers and identify biomarkers. Finally, based on the dynamic changes and trends of potential biomarkers between groups, the mechanism of body damage caused by benzene, toluene, and xylene was initially explored. Results: Urine metabolomics analysis showed that the metabolic profile of urine samples of the benzene, toluene, and xylene combined exposure group was different from that of the control group. 27 potential biomarkers that were closely related to the combined exposure of benzene, toluene, and xylene were screened and identified. These potential biomarkers were enriched in 16 metabolic pathways, of which 3 pathways were significantly enriched (P<0.05) , respectively, lysine metabolism, amino sugar metabolism, and nucleotide sugar metabolism. Conclusion: The metabonomics method can well reflect the changes in the metabolome of urine samples in the occupational population after the combined exposure of benzene, toluene, and xylene, which will help us better evaluate the risk of combined exposure of benzene, toluene, and xylene and prevent and control their health risks.


Assuntos
Benzeno , Xilenos , Benzeno/análise , Biomarcadores , Cromatografia Líquida , Humanos , Espectrometria de Massas , Metabolômica , Tolueno/análise , Xilenos/análise
18.
Artigo em Chinês | MEDLINE | ID: mdl-33910292

RESUMO

Objective: To establish a method for the determination of chlorothalonil in air by filtration membrane sampling, solvent elution and gas chromatography. Methods: PTFE filter was used for sampling, eluted with dichloromethane, separated by DB-5 capillary column, and determined by FID. Results: The standard curve was used for quantitative detection, and the correlation of chlorothalonil in the range of 15 µg/ml-300 µg/ml, R(2)=0.9999. The detection limit of this method was 1.70 µg/ml and the lower quantitative limit was 5.70 µg/ml. The minimum detected concentration was 0.045 mg/m(3) (75L air sample was collected) . The recovery rate was 90.14%-91.81%. The precision of the same batch was 1.5%-1.8%, and that of different batches was 2.3%-3.8%. The sampling efficiency can reach above 95%; The samples can be stored for 14 days at room temperature. Conclusion: The results show that the method of filtration membrane sampling-solvent elude-gas chromatography is suitable for the determination of chlorothalonil in the air.


Assuntos
Poluentes Ocupacionais do Ar , Local de Trabalho , Poluentes Ocupacionais do Ar/análise , Cromatografia Gasosa , Nitrilos , Solventes
19.
Zhonghua Xue Ye Xue Za Zhi ; 42(3): 205-209, 2021 Mar 14.
Artigo em Chinês | MEDLINE | ID: mdl-33910305

RESUMO

Objective: To analyze the clinical manifestation, laboratory examination, treatment and prognosis of congenital factor Ⅺ (FⅪ) deficiency. Methods: The clinical data of 80 patients with congenital FⅪ deficiency in our hospital from September 2006 to October 2020 were analyzed retrospectively. Results: Among the 80 patients, there were 33 males (41.3%) and 47 females (58.8%) , with a median age of 32 (2-66) years. Twenty-eight cases (35.0%) had bleeding events, including 11 cases of spontaneous bleeding (13.8%) , 9 cases of ecchymosis or bleeding after skin trauma (11.3%) , 9 cases of postoperative bleeding (11.3%) . Among the female patients, there were 11 cases of menorrhagia (23.4%) and 1 case of bleeding after vaginal delivery (2.1%) . Laboratory examination were characterized by prolonged activated partial thromboplastin time (APTT) , normal prothrombin time (PT) , and decreased FⅪ activity (FⅪ∶C) . Nine patients (11.3%) were tested for FⅪ gene (F11) with 11 mutations. Twenty-seven patients (33.8%) received fresh frozen plasma (FFP) treatment, 15 patients (18.8%) were received for prophylaxis with no bleeding occurred during and after operation. Conclusion: Most patients with congenital FⅪ deficiency have no or mild bleeding symptoms. There was no significant correlation between FⅪ∶C and the severity of bleeding symptoms, and there was a well consistency between FⅪ∶C and F11 homozygous or heterozygous mutation type. Prophylactic infusion of FFP can effectively reduce the risk of operative bleeding.


Assuntos
Transtornos da Coagulação Sanguínea , Deficiência do Fator XI , Adulto , Idoso , Deficiência do Fator XI/genética , Feminino , Heterozigoto , Homozigoto , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
20.
Zhonghua Xue Ye Xue Za Zhi ; 42(3): 217-223, 2021 Mar 14.
Artigo em Chinês | MEDLINE | ID: mdl-33910307

RESUMO

Objective: To prepare a novel tri-specific T cell engager (19TriTE) targeting CD19 antigen, and to investigate its immunotherapeutic effect on CD19-positive hematological malignancies. Methods: 19TriTE was constructed by molecular cloning technology and successfully expressed through the eukaryotic expressing system. The effects of 19TriTE on the proliferation and activation of T cells, as well as the specific cytotoxicity against CD19 positive tumor cell lines were verified. Results: ①19TriTE expressing plasmid was constructed and successfully expressed through the eukaryotic expressing system. ②19TriTE can specifically bind to T cells and Nalm6 cells, with equilibrium dissociation constants of 19.21 nmol/L and 11.67 nmol/L, respectively. ③The expression rates of CD69 positive T cells and CD25 positive T cells were 35.4% and 49.8% respectively, when 2 nmol/L 19TriTE were added in the co-culture system, which were significantly higher than those in the control group. ④19TriTE can significantly promote the proliferation of T cells. The absolute count of T cells expanded from the initial one million to 74 million with an 74 fold increase at the concentration of 1 nmol/L on day 12. ⑤19TriTE can significantly mediate T cells killing of CD19 positive target cells in a dose-dependent manner. At the concentration of 10 nmol/L, the target cells lysis reached 50%. ⑥Degranulation experiment verified that 19TriTE can activate T cells in the presence of CD19 positive target cells, and the activation of T cells positively correlated with the dose of 19TriTE. ⑦When 19TriTE fusion protein co-cultured with T cells and target cells overexpression RFP and luciferase genes respectively, 19TriTE can notably mediate T cells killing of CD19 positive target cells through fluorescent microscope or bioluminescence imaging technology. Conclusion: In this study, we successfully constructed and expressed 19TriTE fusion protein and verified that it can effectively activate T cells and promote their proliferation in vitro. At the same time, it can bind to CD19 positive target cells and T cells, as well as enhance T cells anti-leukemia effect in vitro, providing the foundation for further clinical research.


Assuntos
Antígenos CD19 , Leucemia , Linhagem Celular Tumoral , Humanos , Imunoterapia Adotiva , Linfócitos T
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