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1.
Nanomaterials (Basel) ; 10(3)2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32143522

RESUMO

Nanosized Mn3O4 nanowires are prepared with KMnO4 and ethanol in mild conditions by facile hydrothermal method. Hydrothermal reaction temperature is optimized to get uniform nanowires. The prepared Mn3O4 nanowires exhibit high activity in the treatment of phenol at acid condition and room temperature. The 20 mg Mn3O4 nanowires can efficiently dispose of 50 mL phenol solution (0.2 g·L-1) at pH 2 and 25 °C. The nanowires before and after phenol treatment are characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) and the reaction mechanism is discussed.

2.
Nanoscale ; 12(7): 4676-4685, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32048702

RESUMO

Platelets play an important role in the early stage of arterial remodeling after injury. Integrin GPIIb/IIIα (αIIbß3) regulates platelet activation in the inside-out and outside-in signaling pathways. The use of tirofiban, an integrin αIIbß3 inhibitor, in clinical therapy is limited by its short in vivo circulation time. Herein, a controlled drug-release system was formulated using CuS@mSiO2-PEG core-shell nanoparticles as near-infrared-triggered nanocarriers to release tirofiban on demand. The nanocarriers possessed good colloidal stability and very high loading efficiency for the integrin αIIbß3 inhibitor (14.5 wt% for tirofiban). Local application of αIIbß3 antagonist-tirofiban on an injured arterial wall inhibited platelet activation, which was accelerated by laser irradiation. Ex vivo platelet-promoted monocyte transmigration trans-well assays revealed decreased monocyte transmigration after platelet activation was inhibited by tirofiban. Two weeks after the wire-induced injury, the intimal area and cellular content were analyzed. The neointimal area was decreased in ApoE-/- mice with CuS@mSiO2-PEG/tirofiban and laser irradiation-promoted tirofiban release, which had limited the neointima formation. The lesions showed a decreased content of macrophages and smooth muscle cells compared with ApoE-/- mice without tirofiban inhibition. Therefore, the action of platelet-integrin αIIbß3 in neointima formation after vascular injury was successfully inhibited in vivo through the controlled release of tirofiban using a near-infrared-triggered nanocarrier, leading to the decrease of early-stage neointima formation. This study also emphasizes the role of platelets in vascular remodeling and provides a new target, namely integrin αIIbß3, for the inhibition of neointimal hyperplasia during vascular inflammation.

3.
Sci Rep ; 10(1): 265, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31937821

RESUMO

Monoculture and improper management may reduce soil fertility and deteriorate soil structure in Black soils (Mollisols) of Northeast China. The experiment was carried out from 2015 to 2016 in Black Soils comprising five cropping systems: continuous corn (CC), soybean-corn rotation (SC), corn-soybean rotation (CS), fallow-corn (FC), and fallow-soybean (FS). Our results showed that CS and FS treatments significantly increased mean weight diameter (MWD) and fractal dimension (D) in mechanical stability aggregates (MSAs), and increased MWD and geometric mean diameter (GMD) in water-stable aggregates (WSAs) compared with CC treatment. These two treatments were also significantly increased water-stable aggregates stability rate (WSAR), but decreased percentage of aggregates destruction (PAD) than CC treatment. Meanwhile, CS and FS treatments exhibited a higher carbon accumulation than CC treatment in bulk soils. Soil organic carbon (SOC) concentration in WSA0.106-0.25,WSA2-5 mm and WSA0.5-1 mm had a dominant effect on aggregate stability. Simutaneously, SOC in WSA>5 mm affected SOC concentration in bulk soils. As a whole, the CS and FS treatments can increase the percentage of macro-aggregates, enhance aggregate stability, as well as increase SOC concentration in bulk soils and all soil aggregate sizes.

4.
Chem Commun (Camb) ; 56(11): 1737-1740, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-31938796

RESUMO

The Pd(0)-catalyzed tandem intermolecular syn-carbopalladation/asymmetric C-H alkenylation reaction of N-ferrocenyl propiolamides with aryl iodides has been realized, generating planar chiral ferrocene[1,2-d] pyrrolinones in good yields. Through employing BINOL-derived phosphoramidite ligands, up to 95% ee is achieved.

5.
Chem Commun (Camb) ; 56(11): 1665-1668, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-31939459

RESUMO

A convenient and efficient palladium-catalyzed C-H bond activation for the assembly of N-aryl carbazole is reported, in which two C-N bonds were formed under one set of conditions. The desired carbazoles were achieved in decent yields with a wide substrate scope by utilizing readily available 2-iodo biphenyls and aromatic amines as starting materials.

6.
Thyroid ; 30(3): 443-450, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31964276

RESUMO

Background: Remarkable achievements have been made for over two decades by implementing a universal salt iodization policy in China. However, changes in the standards and manufacturing of iodized salt have made it necessary to regularly monitor iodine intake and thyroid function in the population. Therefore, we aimed to evaluate iodine nutritional status using thyroid function and urinary iodine levels in pregnant women via a national survey. Methods: Participants included pregnant women enrolled in the 2015 Chinese Adults Chronic Diseases and Nutrition Surveillance (CACDNS). Urinary iodine concentration (UIC) was measured using arsenic and cerium catalysis spectrophotometry. Plasma levels of thyrotropin (TSH), fT4 (free thyroxine), thyroid peroxidase antibodies (TPO-Ab), and thyroglobulin antibodies (TG-Ab) were determined using an automated chemiluminescence immunoassay analyzer. Results: UIC was analyzed in a total of 6173 urinary samples collected from pregnant women residing in 31 provinces of China. The median urinary iodine concentration was 146 µg/L. Pregnant women with UIC >250 µg/L had higher TSH levels than those with UIC 120-149 and 150-249 µg/L. After excluding women with positive TPO-Ab and TG-Ab, a total 2097 plasma samples collected from pregnant women during three periods were analyzed for TSH and fT4. The frequency of normal thyroid function in this study was >85%. The most common type of abnormal thyroid function, subclinical hypothyroidism, was similar to the 2010-2012 China Nutrition and Health Surveillance (CNHS), but its prevalence decreased to 6.8% compared with that in the 2010-2012 CNHS. Compared with pregnant women who had UIC <250 µg/L, the prevalence of subclinical hypothyroidism was significantly increased in those with UIC >250 µg/L. Even with UIC levels 120-149 µg/L, the iodine status of Chinese pregnant women in the 2015 CACDNS is acceptable. Conclusions: The iodine status of pregnant women is generally adequate, but urinary iodine levels are close to the cutoff for suitable iodine status (150 µg/L). With noniodized salt widely available, continued improvement in monitoring of iodine nutritional status and thyroid function is important in this population.

7.
Phys Chem Chem Phys ; 22(5): 3066-3072, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-31965119

RESUMO

Finding novel compounds with unusual crystal structures and physical properties is always an important goal for the materials and chemistry community. Pressure becomes attractive due to its unique ability to break down many fundamental rules by modifying the chemical properties of elements, overcoming reaction barriers and shortening interatomic distances, leading to the formation of some novel materials with unexpected properties. In this work, for the first time we have analyzed the high-pressure phase diagram, crystal structures and electron properties of the Mg-Br system up to 200 GPa using unbiased structure searching techniques. Besides the already known MgBr2, here we report that three unusual stoichiometries of Mg-Br compounds can be stabilized at high pressures as MgBr3, MgBr and Mg4Br. Firstly, among the predicted stable compounds, we find that the Mg4Br in the I4/mmm structure stabilized at 178 GPa behaves as a typical electride, indicating that the formation pressure of an electride for Mg can be significantly reduced by bonding with Br atoms. Secondly, it is surprising that the unexpected oxidation states of Br approaching -2 are observed in the predicted I4/mmm Mg4Br and Pm3[combining macron]m MgBr compounds. Furthermore, P21/m MgBr3 and I4[combining macron]2m MgBr3 phases are predicted as superconductors with an estimated Tc of 23.2 and 0.49 K, respectively. Our work represents a significant step toward understanding the high pressure behaviors of alkaline earth halides and searching for novel high temperature superconductors.

8.
Sci Total Environ ; 707: 136191, 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-31972915

RESUMO

Gully erosion has been widely studied during the rainy season due to soil loss that seriously reduces arable area and decreases soil quality. However, very few publications have focused on gully slope erosion (GSE) during freeze thaw cycle (FTC). In this study, GSE on both active and stable gullies in Mollisol fields was investigated by 3D-photogrammetry. Soil bulk density (BD), soil moisture (SM), soil temperature (ST), daily maximum difference in soil temperature (MDT), saturated water (SW), field capacity (FC), soil organic carbon (SOC), soil total nitrogen (TN), water-stable soil aggregate (WA), vegetation cover rate (VC), root dry weight (RW), root length (RL), slope length (SL) and slope steepness (SS) were compared before- and after FTCs. The main results are as follows: (1) combined with both front and profile views, 3D photogrammetry can be used to monitor GSE; (2) GSE mainly occurred at the early stage of FTCs (approximately 80%) and was mainly determined by snowmelt of both the gully slope and farmland and was driven by the solar radiation in activity gully; (3) the high ST in surface soil layers (0-5 cm) of active gullies accelerated the GSE; (4) GSE on the active gully slope was 7.3-9.8 times greater than that on the stable gully slopes; (5) the plough pan as the important layer can effectively reduce GSE at upper slope positions in an active gully; (6) low values of VC, BD, SOC, RW, RL and macro-WA and high values of SL, SW and MDT in the middle of the gully slope typically accelerate the GSE; (7) the index SS*SL/VC can be used to predict GSE on Mollisol gully slopes. Generally, GSE was greatest after FTCs compare to the soil loss tolerance in the Mollisol region, especially in the middle slope position of the active gully, and should urgently be controlled.

9.
J Hazard Mater ; 384: 121216, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31733995

RESUMO

Black soils (Mollisols) are important soil resources for crop production and maintain food safety in China. For keeping soil fertility, the application of animal manure is commonly practiced in black soils. However, the impact of this application on abundance and diversity of antibiotic resistance genes (ARGs) in black soils of China remains unclear. Here, we surveyed the profiles of ARGs in 72 soil samples collected from four long-term experimental stations with different fertilization regimes and from open farmlands in two sites across northeast China using high-throughput quantitative PCR. Results showed that a total of 178 ARGs including mobile genetic elements (MGEs) were detected, and the diversity and abundance of ARGs were significantly increased with manure application. Additionally, the finding of a significant positive correlation between relative abundance of ARGs and MGEs (P < 0.0001), suggesting that horizontal gene transfer may potentially impact the transmission of ARGs. Furthermore, two genes aadA-1-01 and mexF, encoding resistance to aminoglycoside and multidrug, respectively, were recognized as indicators to estimate the abundance of other co-occurring ARGs. These findings provided insights into the soil resistome in black soils of northeast China and also highlighted the environmental risks caused by manure application should not be ignored.

10.
J Exp Zool B Mol Dev Evol ; 334(1): 25-36, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31743605

RESUMO

Opsins play important roles in the image-forming visual pathways and numerous biological systems such as the biological clock and circadian rhythm. However, the nonvisual opsins involved in nonimage forming process are not clear to date. The aim of this study was to characterize nonvisual opsins in Paralichthys olivaceus. A total of 24 nonvisual opsin genes were identified. Expressions of these genes in eye, brain, heart, testis, and fin were investigated by quantitative real-time polymerase chain reaction (qRT-PCR). Testis contained a surprisingly large number of nonvisual opsins including Opn4m2, Tmt2a, Tmt3b, Opn3, RRH, Opn7a, and Opn7b. Syntenic and phylogenetic analyses confirmed that the RGRa and RGRb originated from the teleost-specific genome duplication (TSGD). qRT-PCR results demonstrated high RGRa and RGRb expression in the eye, while the expression levels in the brain, heart, testis, and fin were relatively weak. In situ hybridization results presented here revealed the presence of both RGRa and RGRb mRNA-positive signals in the ganglion cell layer but absence in the intracellular compartment of retinal pigment epithelium (RPE) and Müller glial cells. Therefore, we hypothesized that RGRa and RGRb had undergone subfunctionalization in P. olivaceus after TSGD. In conclusion, this study provides novel insights into the evolutionary fates of the RGR genes, still, further studies need to be done to explore the mechanism about the lack of RGR genes' expression in RPE.

11.
Sci Rep ; 9(1): 17948, 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31784668

RESUMO

Exploring the transcriptome of crops in response to warming and elevated CO2 (eCO2) is important to gaining insights of botanical adaption and feedback to climate change. This study deployed Illumina sequencing technology to characterize transcriptomic profile of maize plants at the silking stage, which were grown under warming (2 °C higher than ambient temperature) and eCO2 (550 ppm) conditions. The treatment of ambient temperature and ambient CO2 concentration was considered as control (CK). Warming, eCO2 and warming plus eCO2 resulted in 2732, 1966 and 271 genes expressing differently (DEGs) compared to the CK, respectively. Among the DEGs, 48, 47 and 36 gene ontology (GO) terms were enriched in response to warming, eCO2 and warming plus eCO2 compared to the CK, respectively. The majority of genes were assigned to the biological process category and the cellular component category. Elevated CO2 significantly inhibited gene expressions in terms of photosynthesis and carbohydrate biosynthesis pathways. Warming not only negatively affected expressions of these genes, but also secondary pathways of nitrogen (N) metabolism, including key enzymes of GST30, GST7, GST26, GST15, GLUL and glnA. These results indicated the negative biochemical regulation and physiological functions in maize in response to warming and eCO2, highlighting the necessity to improve the genetic adaptability of plant to future climate change.

12.
Front Microbiol ; 10: 2184, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31681180

RESUMO

The amendment of crop residues produced under elevated CO2 (eCO2) may alter soil microbial community structure and their functions on residue decomposition and carbon (C) cycling in soil. The key to understanding this process is to elucidate the structure of prokaryotic communities that metabolize crop residues derived from eCO2. A soil incubation experiment was conducted to explore the response of soil microbial community to the amendment of 13C-labeled soybean residues produced under ambient CO2 (aCO2) and eCO2 . The residues were applied to a Mollisol, followed by 13C-DNA stable isotope probing (SIP) and Illumina sequencing on soil prokaryotic community over time. The structure of residue-metabolizing community differed in response to the amendment of eCO2- and aCO2-derived residues after 28 days of incubation. In particular, genera Actinomadura, Nocardia, Non-omuraea, and Shimazuella were the dominant members of the residue-metabolizing bacteria, which contributed to this difference. The relative abundances of genera Actinomadura, Nocardia and Shimazuella were 118-144%, 71-113%, and 2-4-fold higher in the Mollisol amended with aCO2-derived than eCO2-derived residue. In contrast, the relative abundance of Non-omuraea was 87-90% greater in the eCO2-residue treatment. However, during the incubation period, there was no difference between the two residue treatments in the community structure as a whole without SIP. These results implied that a pioneering prokaryotic community metabolized the residue initially prior to the entire community. Those bacteria genera being inhibited with the amendment of the eCO2-derived residue, compared to aCO2-derived residue, were likely preferential to metabolize recalcitrant C, which might be associated with changes of chemical composition of the residue under eCO2.

13.
J Vasc Surg ; 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31676177

RESUMO

OBJECTIVE: This study investigated the outcomes of emergency in situ laser fenestration (ISLF)-assisted thoracic endovascular aortic repair (TEVAR) for patients with acute Stanford type A aortic dissection unfit for open surgery. METHODS: Twenty patients with acute Stanford type A aortic dissection who were found to be unfit for open surgery, underwent emergency ISLF-assisted TEVAR in our center between March 2016 and December 2018. Anatomic criteria for endovascular repair: coronary artery and aortic valve was not involved, proximal landing zone diameter of 45 mm or less, and proximal landing zone length of 20 mm or greater. Their clinical outcomes were reviewed retrospectively. RESULTS: Twenty patients achieved a procedural success of 100.0%. The 30-day mortality was 10%; two patients died, one of severe pneumonia and the other from cerebral hemorrhage after the operation. Rate of stroke at 30 days was 5%. The average follow-up time was 16 months (range, 3-26 months). One death owing to heart failure occurred at 23 months postoperatively. Kaplan-Meier curve analysis revealed that the 24-month survival rate was 77.1%. Two patients had type Ia endoleaks and one had a type II endoleak. There was no stent graft migration or fenestration-related endoleak and all patients had a thrombosed false lumen in the covered section of the stent grafts. No reintervention, myocardial infarction, transient ischemic attacks, cerebral infarction, or other complications occurred during the follow-up period. CONCLUSIONS: Emergency ISLF-assisted TEVAR is a safe and effective alternative method for treating acute Stanford type A aortic dissection unfit for open surgery.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31740945

RESUMO

OBJECTIVES: The modified extracardiac Fontan of direct total cavopulmonary connection (dTCPC) with entirely autologous vessels is a better solution in selected patients with functionally univentricular hearts because it combines the advantages of a tubular connection and of full growth potential. We investigated the mid-term to long-term outcomes of the physical condition of patients who had the dTCPC and assessed the growth potential of the autologous dTCPC pathway. METHODS: From July 2005 to June 2014, 31 patients, aged 1.8-14.0 years, underwent a modified extracardiac Fontan with dTCPC at our institution. Twenty-two patients underwent a 1-stage dTCPC and 9 patients underwent a 2-stage dTCPC. The preoperative and postoperative data were reviewed retrospectively. RESULTS: There were 17 (54.8%) boys and 14 (45.2%) girls, with a median age of 6.4 years (range 1.8-14.0 years) and a mean weight of 17.8 kg (range 9-41 kg). The mean follow-up period was 6.0 years (range 2.1-10.2 years). There was 1 early death and 2 late deaths. The event-free survival rate for the 31 patients was 88.9% at 10 years during the follow-up period. Late protein-losing enteropathy, thromboembolism, arrhythmia or heart failure were not observed. There was a significant difference between the preoperative and postoperative data for body mass index and the diameters of the autologous dTCPC pathway and pulmonary artery branches, confirming that the growth potential of the autologous dTCPC pathway was realized. CONCLUSIONS: The dTCPC procedure could be performed with satisfactory midterm to long-term outcomes in selected patients. It has the potential of retaining the advantage of the extracardiac Fontan operation together with the potential for growth and the avoidance of prosthetic materials.

15.
J Asian Nat Prod Res ; : 1-13, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31762317

RESUMO

Many kinds of drugs induce pseudo-allergic reactions due to activation of mast cells. We investigated the anti-pseudo-allergic effect of andrographolide (Andro). The effects of Andro on pseudo-allergic reactions were investigated in vivo and in vitro. Andro suppressed compound 48/80 (C48/80) induced pseudo-allergic reactions in mice in a dose-dependent manner. Andro also inhibited C48/80-induced local inflammatory reactions in mice. In vitro studies revealed that Andro reduced C48/80-induced mast cells degranulation. Human phospho-kinase array kit and western blotting showed that Andro could inhibit pseudo-allergic responses via the calcium signaling pathway.

16.
Sci Total Environ ; 694: 133784, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31756809

RESUMO

Elevated atmospheric CO2 concentration (eCO2) exerts significant influence on nutrient requirement in plant. The investigation of C:N:P ratios in major cropping soils is important for managing nutrient balance and maximizing their use efficiency in future farming systems. This study aimed to examine the effect of eCO2 on the C:N:P ratios in different plant parts among soybean cultivars. Twenty-four soybean cultivars were planted in open top chambers at two CO2 concentrations (390 and 550 ppm) and sampled at the initial pod filling stage (R5) and the full maturity stage (R8). The C, N and P concentrations in root, stem, leaf and seed were determined. Elevated CO2 decreased the N concentrations in stem (-5.1%) and leaf (-3.2%) at R5, and in root (-24%), stem (-25%) and seed (-6.2%) at R8, resulting in a significant decrease of C:N ratio in the corresponding parts. The P concentration was significantly increased in root (6.0%), stem (7.9%) and leaf (16%) at R5, and in root (2.6%), stem (29%) and seed (16%) at R8 across 24 cultivars, leading to a decrease in the C:P ratio. Elevated CO2 significantly decreased the N:P ratio in root (-4.5%), stem (-12%) and leaf (-17%) at R5, and in root (-26%), stem (-57%) and seed (-22%) at R8. Furthermore, the response of C:N:P ratios to eCO2 varied greatly among soybean cultivars leading to significant CO2 × cultivar interactions. Nitrogen, but not P was the limiting factor for the soybean plants grown in Mollisols under eCO2. The considerable variation in the C:N:P ratios among cultivars in response to eCO2 indicates a potential improvement in soybean adaptability to climate change via selection new cultivars. Cultivars SN22 and ZH4 that did not considerably altered the C:N and C:P ratios in response to eCO2 are likely the optimal genomes in soybean breeding programs for eCO2 adaption.


Assuntos
Dióxido de Carbono/metabolismo , Soja/metabolismo , Biomassa , Mudança Climática , Fabaceae , Nitrogênio , Folhas de Planta , Sementes , Solo
17.
J Vasc Surg Cases Innov Tech ; 5(3): 379-383, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31453422

RESUMO

Patients with persistent sciatic artery are at high risk for development of limb ischemia, aneurysm formation, and embolism. In this report, we identify a nonclassified left leg persistent sciatic artery in a patient with chronic limb ischemia. Vascular reconstruction was carried out by common iliac-deep femoral artery bypass to restore adequate arterial flow. Our approach to placement of the distal anastomosis on the deep femoral artery instead of on the popliteal artery, which is used in routine practice, may potentially increase treatment efficacy and decrease surgical complications. At 2-year follow-up, the patient remained asymptomatic and in good health.

18.
Front Microbiol ; 10: 1424, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31293554

RESUMO

Bacillus-like species are gram-positive bacteria that are ubiquitous in soils. Many of Bacillus-like bacteria are demonstrated as beneficial microbes widely used in industry and agriculture. However, the knowledge related to their diversity and distribution patterns in soils is still rudimentary. In this study, we developed a combined research method of using culture-dependent and high-throughput sequencing to investigate the composition and diversity of cultivable Bacillus-like bacterial communities across 26 soil samples obtained from the black soil zone in northeast China. Nearly all bacterial 16S rDNA sequences were classified into the order Bacillales. Fifteen genera were detected, with Bacillus, Paenibacillus, and Brevibacillus being the three most abundant genera. Although more than 2,000 OTUs were obtained across all samples, 33 OTUs were confirmed as the abundant species with a relative abundance over 5% in at least one sample. Pairwise analysis showed that the diversity of Bacillus-like bacterial communities were significantly and positively correlated with soil total carbon contents and soil sampling latitudes, which suggests that a latitudinal gradient diversity of Bacillus-like bacterial communities exists in the black soil zone. The principal coordinates analysis revealed that the Bacillus-like bacterial communities were remarkably affected by soil sampling latitudes and soil total carbon content. In general, this study demonstrated that a distinct biogeographic distribution pattern of cultivable Bacillus-like bacterial communities existed in the black soil zone, which emphasizes that the strategy of local isolation and application of beneficial Bacillus-like strains is rather important in black soil agriculture development.

19.
Chem Commun (Camb) ; 55(61): 8943-8946, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31287468

RESUMO

An oxidative cyclization of ß,γ-unsaturated hydrazones for the divergent assembly of pyrazolones and pyrazolines under metal-free conditions is presented. This divergent synthetic strategy was achieved by controlling the reaction atmosphere. A number of pyrazolines were obtained via 5-exo-trig cyclization when the reaction was performed under N2. Whereas when the reaction was conducted under O2, diverse functionalized pyrazolones were achieved via oxidative cleavage of the C[double bond, length as m-dash]C double bond.

20.
Hum Gene Ther Clin Dev ; 30(3): 142-148, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31310159

RESUMO

The purpose of this study was to investigate the expression level of microRNA-4513 (miR-4513) in gastric cancer (GC), and to elucidate the mechanisms underlying its regulation of GC progression. Quantitative real-time PCR (qRT-PCR) was performed to measure the expression level of miR-4513 in GC cells. Transfection efficacy of synthetic miRNAs was examined by qRT-PCR. After synthetic miRNA transfection, cell counting kit-8 assay and transwell invasion assay were conducted to measure biological changes in these groups. The key molecular expression level involved in epithelial-mesenchymal transition (EMT) was analyzed by Western blot. Bioinformatic analysis and Western blot were performed to investigate the connection between miR-4513 and lysine acetyltransferase 6B (KAT6B). qRT-PCR results showed that miR-4513 expression level was upregulated in GC cell lines. Downregulation of miR-4513 expression inhibited GC cell proliferation, invasion, and EMT. KAT6B was validated as a direct target of miR-4513. In addition, KAT6B expression level can be upregulated by miR-4513 inhibitor. Collectively, we showed that miR-4513 is involved in regulating the biological function of GC cells via KAT6B. In addition, miR-4513 may serve as a potential target for the molecular therapy of GC.

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