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1.
J Hazard Mater ; 443(Pt A): 130167, 2023 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-36270188

RESUMO

Due to the coexistence of various heavy metals in the contaminated environment, it is essential to comprehensively study the multicomponent adsorption of heavy metals in order to tackle these combined pollutants. Herein, the adsorption processes of Pb(Ⅱ), Cu(Ⅱ) and Cd(Ⅱ) by biogenic hydroxyapatite (BHAp) were investigated in single and multicomponent systems. The maximum adsorption capacity for Pb(Ⅱ), Cu(Ⅱ) and Cd(Ⅱ) by BHAp in single system reached 311.16, 82.05 and 92.54 mg g-1, respectively, while adsorption capacity for Cu(Ⅱ) and Cd(Ⅱ) in multicomponent system decreased more obviously than that of Pb(Ⅱ). Furthermore, the stability of Cu(Ⅱ) and Cd(Ⅱ) adsorbed on BHAp was indeed influenced in multicomponent system. By means of the characterization analysis, it was found that ion exchange was more instrumental in the adsorption processes of Cu(Ⅱ) and Cd(Ⅱ) in single system than in multicomponent system. Significantly, it was observed that the proportion of generally stable Pb(II) adsorbed on BHAp exceeded 95% in both single and multicomponent systems. This result might be due to the in-site growth of stable crystals of PbxCa10-x(PO4)6(OH)2, which was synergistically induced by surface functional groups and inorganic mineral of BHAp, and was unaffected by the coexistence of Cu(Ⅱ) and Cd(Ⅱ).


Assuntos
Metais Pesados , Poluentes Químicos da Água , Adsorção , Chumbo , Cádmio/análise , Durapatita , Metais Pesados/química , Poluentes Químicos da Água/análise , Concentração de Íons de Hidrogênio
2.
J Sci Food Agric ; 103(1): 118-124, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35811466

RESUMO

BACKGROUND: Quinoa is a good gluten-free resource for food processing, especially bread making, and can improve and prevent the development of complications associated with celiac disease (CD). However, lack of gluten affects quinoa bread quality. Previous research showed that soy protein isolate (SPI) could improve gluten-free bread quality to some extent. Therefore, this study investigated the effects of SPI on the physical properties of quinoa dough and gluten-free bread quality characteristics. RESULTS: Results showed that, with appropriate SPI substitution, the farinograph properties of quinoa flour significantly improved (P < 0.05). The sample with 8% SPI substitution showed a better development time (DT, 3.30 ± 0.20 min), stability time (ST, 8.80 ± 0.10 min) and softening degree (SD, 8.80 ± 0.10 FU), which were close to those of wheat flour, although more water absorption (WA, 76.40 ± 2.10%) was needed than for wheat flour (66.30 ± 3.10%). The extensograph properties of quinoa flour also significantly improved after 8% SPI substitution (P < 0.05). Furthermore, SPI substitution increased G' moduli of quinoa dough and decreased tan δ to some extent, providing better rheological properties closer to those of wheat dough. SPI substitution also improved the quality and texture of quinoa bread and reduced the gap with wheat bread. When SPI substitution was 8%, the specific volume, hardness and springiness of quinoa bread were 2.29 ± 0.05 mL g-1 , 1496.47 ± 85.21 g and 0.71 ± 0.03%, respectively. CONCLUSION: These results suggested that SPI substitution would be an effective way to develop higher-quality gluten-free bread. © 2022 Society of Chemical Industry.


Assuntos
Pão , Chenopodium quinoa , Farinha , Proteínas de Soja/química , Triticum/química , Glutens/química
3.
JACC Asia ; 2(1): 33-43, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36340248

RESUMO

Background: Updated American or Chinese guidelines recommended calculating atherosclerotic cardiovascular disease (ASCVD) risk using the Pooled Cohort Equations (PCE) or Prediction for Atherosclerotic Cardiovascular Disease Risk in China (China-PAR) models; however, evidence on performance of both models in Asian populations is limited. Objectives: The authors aimed to evaluate the accuracy of the PCE or China-PAR models in a Chinese contemporary cohort. Methods: Data were extracted from the CHERRY (CHinese Electronic health Records Research in Yinzhou) study. Participants aged 40 to 79 years without prior ASCVD at baseline from 2010 to 2016 were included. ASCVD was defined as nonfatal or fatal stroke, nonfatal myocardial infarction, and cardiovascular death. Models were assessed for discrimination and calibration. Results: Among 226,406 participants, 5362 (2.37%) adults developed a first ASCVD event during a median of 4.60 years of follow-up. Both models had good discrimination: C-statistics in men were 0.763 (95% confidence interval [CI]: 0.754-0.773) for PCE and 0.758 (95% CI: 0.749-0.767) for China-PAR; C-statistics in women were 0.820 (95% CI: 0.812-0.829) for PCE and 0.811 (95% CI: 0.802-0.819) for China-PAR. The China-PAR model underpredicted risk by 20% in men and by 40% in women, especially in the highest-risk groups. However, PCE overestimated by 63% in men and inversely underestimated the risk by 34% in women with poor calibration (both P < 0.001). After recalibration, observed and predicted risks by recalibrated PCE were better aligned. Conclusions: In this large-scale population-based study, both PCE and China-PAR had good discrimination in 5-year ASCVD risk prediction. China-PAR outperformed PCE in calibration, whereas recalibration equalized the performance of PCE and China-PAR. Further specific models are needed to improve accuracy in the highest-risk groups.

4.
Cell Discov ; 8(1): 121, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36344493

RESUMO

Human pluripotent stem cell (hPSC)-induced NK (iNK) cells are a source of off-the-shelf cell products for universal immune therapy. Conventional methods for iNK cell regeneration from hPSCs include embryoid body (EB) formation and feeder-based expansion steps, which are time-consuming and cause instability and high costs of manufacturing. Here, we develop an EB-free, organoid aggregate method for NK cell regeneration from hPSCs. In a short time-window of 27-day induction, millions of hPSC input can output over billions of iNK cells without the necessity of NK cell expansion feeders. The iNK cells highly express classical toxic granule proteins, apoptosis-inducing ligands, as well as abundant activating and inhibitory receptors. Functionally, the iNK cells eradicate human tumor cells via mechanisms of direct cytotoxicity, apoptosis, and antibody-dependent cellular cytotoxicity. This study provides a reliable scale-up method for regenerating human NK cells from hPSCs, which promotes the universal availability of NK cell products for immune therapy.

5.
Sci Rep ; 12(1): 18489, 2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36323798

RESUMO

The cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), an important pest of cotton, is detrimental to cotton production. Light from UV-A ultraviolet lamps is regarded as a form of environmental stress for insects. In order to investigate the response of H. armigera exposed to UV-A, we explored Hap38 MAPK expression and functions. We hope that the findings of this study will lay the foundation for future investigations into the insect's phototaxis mechanism. A p38 MAPK was cloned and named Hap38 MAPK. A phylogenetic tree showed that Hap38 MAPK was highly conserved. The gene was highly expressed in the thorax and females. Under UV-A stress, the expression of the gene decreased significantly. After silencing Hap38 MAPK, the activity of the antioxidant enzymes SOD, POD, CAT, and GR decreased. This study suggested that Hap38 MAPK responds to UV-A irradiation and plays critical roles in the defense response to environmental stresses.


Assuntos
Mariposas , Animais , Feminino , Filogenia , Mariposas/genética , Larva
6.
J Agric Food Chem ; 2022 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-36417897

RESUMO

The antiaging effects of sea red rice bran in vivo, a new saline-tolerant sea rice byproduct containing high levels of ceramides (Cers), remain unknown. This study aimed to explore the antiaging effects exerted by Cers from sea red rice bran on Caenorhabditis elegans, assess its health indicators as well as tolerance, and then reveal the mechanism of action of Cers in prolonging the mean life span through genetic studies. The results indicated that the mean life span of Cers-treated C. elegans were dose-dependent in the range of 0.10-0.50 mg/mL. Additionally, Cers improved nematode motility, reduced lipofuscin accumulation, and enhanced resistance to heat stress and antioxidant enzyme activity. Genetic studies showed that Cers treatment had altered nematode gene expression. In addition, insulin/IGF-1 and jnk-1/mitogen-activated protein kinase (MAPK) signaling pathways successfully demonstrated the longevity effects of Cers intake. In short, these results suggest that Cers enhance the resistance of C. elegans and prolong its life span.

7.
Ying Yong Sheng Tai Xue Bao ; 33(10): 2705-2710, 2022 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-36384605

RESUMO

The reactive nitrogen deposition in subtropical region of China has been increasing annually, which affects biogeochemical processes in forest soils. In this study, three treatments were established, including control (no N addition, CK), low nitrogen deposition (40 kg·hm-2·a-1, LN), and high nitrogen deposition (80 kg·hm-2·a-1, HN) to study the response of denitrifying functional genes and potential N2O emissions to simulated nitrogen deposition in the soils of a natural Castanopsis carlesii forest. Results showed that HN significantly decreased soil potential N2O emission, while 8-year nitrogen deposition did not affect the abundances of nirS, nirK, nosZ Ⅰ and nosZ Ⅱ. However, the abundance of nosZ Ⅰwas significantly higher than nosZ Ⅱ in all the treatments, indicating that nosZ Ⅰ dominated over nosZ Ⅱ in the acidic soils. HN significantly decreased the ratio of (nirK+nirS)/(nosZ Ⅰ+nosZ Ⅱ), which was positively correlated with soil pH. The results suggested that long-term high nitrogen deposition reduced soil pH and the abundance ratio of (nirK+nirS)/(nosZ Ⅰ+nosZ Ⅱ), which subsequently reduced the potential N2O emission.


Assuntos
Óxido Nitroso , Solo , Óxido Nitroso/análise , Nitrogênio , Microbiologia do Solo , Desnitrificação , Florestas
8.
Ying Yong Sheng Tai Xue Bao ; 33(10): 2711-2717, 2022 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-36384606

RESUMO

At the regional scale, substrate properties are the key factors driving litter decomposition rate. In this study, soil temperature was increased by buried heating cables to explore the impacts of climate warming on the physical and chemical properties in branch and leaf of Cunninghamia lanceolata litter. The results showed that after 5 years of soil warming (4 ℃), the contents of nitrogen (N), phosphorus (P) and water-soluble substance in branch litter increased by 35.2%, 40.8% and 7.6%, while that in leaf litter increased by 41.2%, 45.9% and 5.9%, respectively. The contents of carbon (C), cellulose and C/N in branch litter decreased by 5.1%, 11.6% and 28.8%, and in leaf litter decreased by 5.3%, 11.3% and 33.3%, respectively. Soil warming led to 29.8% increase in specific leaf area (SLA) and 40.7% decrease in tensile strength (LTS) of leaf litter. However, warming did not affect lignin content and pH value in both branch and leaf litter. 13C NMR and infrared spectrum analysis showed that the contents of amino acids, polysaccharides, polyphenols and aliphatic compounds in litter changed significantly after warming. Warming effect differed between litter organs, in that polysaccharides increased significantly only in leaf litter and the increase of amino acids in branch litter was greater than that in leaf litter. Overall, soil warming significantly changed the physical and chemical properties in C. lanceolata branch and leaf litter, which might accelerate the decomposition rate at the initial stage due to the increase of N, P contents and the decrease of LTS, but might decelerate the decomposition rate at the later stage due to an increase of complex polymers content in the litter.


Assuntos
Cunninghamia , Folhas de Planta/química , Solo/química , Carbono/análise , Aminoácidos/análise
9.
Cell Rep ; 41(5): 111569, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36323259

RESUMO

Innate lymphoid cells (ILCs) play important roles in regulating tissue homeostasis and innate immune responses. Generation of ILCs after engraftment of pluripotent stem cell (PSC)-derived hematopoietic progenitors (iHPCs) has not yet been reported. Here, we document that ILCs exist in Rag2-/-Il2rg-/- recipients engrafted with PSC-derived iHPCs guided by Runx1 and Hoxa9 expression. Upon transplantation, iHPCs immediately give rise to ILC-related progenitors containing common helper ILC progenitors in the bone marrow, followed by a more restricted population named ILC progenitors, which are able to further differentiate into mature ILCs in the primary and secondary immunodeficient recipients. The PSC-derived ILCs exhibit multiple tissue distributions and normal immunological functions. Single-cell transcriptomics illustrates the developmental trajectory of PSC-derived ILCs in vivo, which is consistent with that of natural ILCs. Our study provides insights into the generation of ILCs in animals transplanted with PSC-derived iHPCs as a cell source.


Assuntos
Imunidade Inata , Células-Tronco Pluripotentes , Animais , Linfócitos/metabolismo , Diferenciação Celular , Células Progenitoras Linfoides/metabolismo
10.
World J Surg Oncol ; 20(1): 331, 2022 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-36192752

RESUMO

BACKGROUND: Mitochondria play critical roles in cellular physiological activity as cellular organelles. Under extracellular stimulation, mitochondria undergo constant fusion and fission to meet different cellular demands. Mitochondrial dynamics, which are involved in mitochondrial fusion and fission, are regulated by specialized proteins and lipids, and their dysregulation causes human diseases, such as cancer. The advanced literature about the crucial role of mitochondrial dynamics in breast cancer is performed. METHODS: All related studies were systematically searched through online databases (PubMed, Web of Science, and EMBASE) using keywords (e.g., breast cancer, mitochondrial, fission, and fusion), and these studies were then screened through the preset inclusion and exclusion criteria. RESULTS: Eligible studies (n = 19) were evaluated and discussed in the systematic review. These advanced studies established the roles of mitochondrial fission and fusion of breast cancer in the metabolism, proliferation, survival, and metastasis. Importantly, the manipulating of mitochondrial dynamic is significant for the progresses of breast cancer. CONCLUSION: Understanding the mechanisms underlying mitochondrial fission and fusion during tumorigenesis is important for improving breast cancer treatments.


Assuntos
Neoplasias da Mama , Dinâmica Mitocondrial , Neoplasias da Mama/patologia , Transformação Celular Neoplásica/patologia , Feminino , Humanos , Lipídeos , Mitocôndrias/metabolismo , Dinâmica Mitocondrial/fisiologia , Proteínas Mitocondriais/metabolismo
11.
Food Chem X ; 15: 100444, 2022 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-36211733

RESUMO

The gut microbiota dysbiosis is a state which the physiological combinations of flora are transformed into pathological combinations caused by factors such as diets, pollution, and drugs. Increasing evidence shows that dysbiosis is closely related to many diseases. With the continuous development and utilization of marine resources, marine polysaccharides have been found to regulate dysbiosis in many studies. In this review, we introduce the types of dysbiosis and the degree of it caused by different factors. We highlight the regulating effects of marine polysaccharides on dysbiosis as a potential prebiotic. The mechanisms of marine polysaccharides to regulate dysbiosis including protection of intestinal barrier, regulatory effect on gut microbiota, alteration for related metabolites, and some other possible mechanisms were summarized. And we aim to provide some references for the high-value utilization of marine polysaccharides and new targets for the treatment of gut microbiota dysbiosis by this review.

12.
Int J Mol Sci ; 23(19)2022 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-36232856

RESUMO

As a result of antibiotic overuse, bacterial antibiotic resistance has become a severe threat to worldwide public health. The development of more effective antimicrobial therapies and alternative antibiotic strategies is urgently required. The role played by bacterial membrane vesicles (BMVs) in antibiotic resistance has become a current focus of research. BMVs are nanoparticles derived from the membrane components of Gram-negative and Gram-positive bacteria and contain diverse components originating from the cell envelope and cytoplasm. Antibiotic stress stimulates the secretion of BMVs. BMVs promote and mediate antibiotic resistance by multiple mechanisms. BMVs have been investigated as conceptually new antibiotics and drug-delivery vehicles. In this article, we outline the research related to BMVs and antibiotic resistance as a reference for the intentional use of BMVs to combat antibiotic resistance.


Assuntos
Anti-Infecciosos , Bactérias , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Membrana Celular , Farmacorresistência Bacteriana , Farmacorresistência Bacteriana Múltipla
13.
Biotechnol Lett ; 44(12): 1477-1493, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36315299

RESUMO

OBJECTIVES: As a type of agricultural waste, there is a large amount of lignocellulose in corn (Zea mays) straw, but it is difficult to utilize efficiently owing to its recalcitrance to enzymatic degradation. Three strains of actinomycetes that degrade cellulose were constructed as complex flora, and the conditions of cellulose degradation conditions and their degradative activity were optimized and evaluated. RESULTS: When the complex flora were inoculated into the fermentation medium at pH 7 and 3% (v/v), the rate of degradation of corn straw reached 38.24% after 5 d of fermentation at 28 ºC and 180 rpm. Cellulose, hemicellulose, and lignin in the corn straw were degraded by 33.97%, 34.08%, and 21.52%, respectively. The results from scanning electron microscopy showed that the waxy layer on the surface of corn straw became thin and gradually disappeared following fermentation by the complex flora. Fourier-transform infrared spectroscopy showed that the complex flora could change the internal functional groups of corn straw at different fermentation periods. The compounds detected in the fermentation system indicated that the corn straw was efficiently degraded. CONCLUSIONS: These results indicated that the constructed complex flora was more effective at degrading corn straw than the individual strains and provides research concepts for the development and utilization of biomass resources.


Assuntos
Actinobacteria , Zea mays , Zea mays/química , Actinobacteria/metabolismo , Actinomyces , Celulose/metabolismo , Fermentação
14.
Life Sci ; 311(Pt A): 121105, 2022 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-36272467

RESUMO

AIMS: In this study, we synthesized a 10-fluorine-substitution derivative of CPT (Camptothecin) YCJ100 and evaluated its antitumor activity and systemic toxicity. MATERIALS AND METHODS: Determination of in vitro antitumor activity and mechanism of YCJ100 by the MTT assay, Molecular docking, EdU staining, Cell cycle and apoptosis determination, Western blot analysis and Topoisomerase I activity assay. The antitumor effects of YCJ100 were evaluated in primary HCC (hepatocellular carcinoma), ICC (intrahepatic cholangiocarcinoma) mouse models, and pancreatic cancer xenograft models. KEY FINDINGS: YCJ100 showed superior cytotoxic activity compared to Topotecan in SW480, SW1990, Hep3B, HepG2, A549, A2780, HeLa, and QBC cells. YCJ100 blocked the cell cycle in the G2/M phase, inhibited cell proliferation and induced apoptosis in HepG2 and SW1990 cells. Mechanistically, YCJ100 inhibited topoisomerase I activity in both a cell-free system and a cellular system, similar to the mechanism of Topotecan. YCJ100 showed significant antitumor activity and was more potent than Topotecan in primary HCC and ICC mouse models, as well as a xenograft mouse model. Additionally, YCJ100 showed only minor toxicity to the mouse hematopoietic system, liver, and kidney. These findings indicate that YCJ100 has high antitumor activity and low systemic toxicity. SIGNIFICANCE: Our findings demonstrate that YCJ100, as a Topoisomerase I inhibitor, has in vitro and in vitro antitumor activity. This study provides a new lead compound worthy of further preclinical evaluation and potential clinical development.

15.
Curr Res Food Sci ; 5: 1897-1905, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36276241

RESUMO

Heparinoid, a sulfate polysaccharide derived from marine organisms was attracted largely attention due to its versatile activities. A naturally occurring heparinoid (M2) that was extracted from the mollusk Meretrix lusoria and used in this investigation shown strong antithrombotic action. UV-Vis, FT-IR, SAX-HPLC, and NMR were used to explore the structural characteristics of M2, results indicated that M2 similar with heparin, its average molecular weight was 22.58 kDa. Which was primarily made up of→4)-α-IdoA2S-(1→4)-α-GlcNS6S-(1→ (31.19%), →4)-ß-GlcA-(1→4)-α-GlcNAc (1→ (23.21%), →4)-ß-GlcA-(1→4)-α-GlcNS (1→ (13.87%), →4)-α-IdoA2S-(1→4)-α-GlcNS (1→ (8.95%), →4)-ß-GlcA-(1→4)-α-GlcNAc6S (1→ (7.39%) and →4)-ß-GlcA-(1→4)-α-GlcNS6S (1→ (7.63%). The antithrombotic activity of M2 was evaluated using measurements of the anticoagulant effect in vitro and the fibrinolytic capability in vitro and in vivo, and M2 has 122.4 U/mg of anticoagulant activity and 1.41 U/mg of fibrinolytic activity, respectively. Additionally, a mouse tail-cutting model was used to assess the bleeding effect in real time, it found that M2 had a reduced hemorrhagic risk than heparin. Consequently, M2 could be exploited to develop functional foods or medications with antithrombotic properties.

16.
Micromachines (Basel) ; 13(10)2022 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-36295955

RESUMO

The long-term application of sensors in a high-temperature environment needs to address several challenges, such as stability at high temperatures for a long time, better wiring interconnection of sensors, and reliable and steady connection of the sensor and its external equipment. In order to systematically investigate the reliability of thin coatings at high temperatures for a long time, Au and Cr layers were deposited on silicon substrates by magnetron sputtering. Additionally, samples with different electrode thicknesses were annealed at different temperatures for a varied duration to study the effect of electrode thickness, temperature, and duration on the reliability of samples. The results of tensile and probe tests before and after heat treatment revealed that the mechanical strength and electrical properties have changed after annealing. In addition, the bonding interface was analyzed by a cross-sectional electron microscope. The analysis showed that long-term continuous high-temperature exposure would result in thinning of the electrode, formation of pores, recrystallization, and grain growth, all of which can affect the mechanical strength and electrical properties. In addition, it was observed that increasing the thickness of the gold layer will improve reliability, and the test results show that although the thin metal layer sample is in poor condition, it is still usable. The present study provides theoretical support for the application of thin coatings in high temperatures and harsh environments.

17.
J Org Chem ; 2022 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-36273332

RESUMO

Electroreductive ring-opening carboxylation of cycloketone oxime esters with atmospheric carbon dioxide is reported. This reaction proceeded under simple constant current conditions in an undivided cell using glassy carbon as the cathode and magnesium as the sacrificial anode, providing substituted γ- and δ-cyanocarboxylic acids in moderate to good yields. Electrochemically generated cyanoalkyl radicals and cyanoalkyl anion are proposed as the key intermediates.

18.
Crit Rev Food Sci Nutr ; : 1-26, 2022 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-36315047

RESUMO

According to the World Health Organization, cardiovascular disease (CVD) has become a major cause of chronic illness around the globe. It has been reported that soy-based fermented food (SFF) is very effective in preventing thrombus (one of the most important contributing factors to CVD), which are mainly attributed to the bioactive substances, especially the fibrinolytic enzymes (FE) generated by microorganisms during the fermentation process of soybean food. This paper therefore mainly reviewed the microbial fibrinolytic enzymes (MFE) from SFF. We first discuss the use of microbial fermentation to produce FE, with an emphasis on the strains involved. The production, purification, physicochemical properties, structure-functional attributes, functional properties and possible application of MFE from SFF are then discussed. Finally, current limitations and future perspectives for the production, purification, and the practical application of MFE are discussed. MFE from SFF pose multiple health benefits, including thrombolysis, antihypertension, anti-inflammatory, anti-hyperlipidemia, anticancer, neuroprotective, antiviral and other activities. Therefore, they exhibit great potential for functional foods and nutraceutical applications, especially foods with CVDs prevention potential.

19.
Dalton Trans ; 51(37): 14054-14058, 2022 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-36106962

RESUMO

Introducing sulfonic acid groups into MOF materials is one of the effective approaches to enhance proton conduction. Here, we attempted to prepare a new post-modified ZIF-90-based material by addition reaction of the aldehyde group with bisulfite to obtain partially functionalized ZIF-90-SO3Na(2.3). ZIF-90-SO3Na(2.3) exhibits a high proton conductivity of 2.26 × 10-2 S cm-1 at 98% RH and 100 °C.

20.
J Sci Food Agric ; 2022 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-36088619

RESUMO

BACKGROUND: Soybean 11S globulin has good functional properties, which are widely used in the field of food. However, natural soybean 11S globulin (N-11S) has low flexibility and is easy to aggregate, impacting its foaming process. Studies have shown that soybean 11S globulin in molten globule state (MG-11S) has better molecular flexibility than N-11S, and trehalose has been shown to improve the properties of proteins. Therefore, this study investigated the interaction mechanism between trehalose and MG-11S, and its impact on rheological and foaming properties of MG-11S. RESULTS: The molecular docking and intrinsic fluorescence results showed that hydrogen bonding was the main interaction force at lower than 0.5 mol L-1 trehalose added. Meanwhile, rheology and foaming showed that the MG-11S-trehalose complexes had better viscoelasticity, foaming ability (66.67-86.67%) and foaming stability (75.00-89.29%) than N-11S (16.67% foaming ability and 40.00% foaming stability); however, when the trehalose was higher than 0.5 mol L-1 , molecular crowding occurred and H-bonds were weakened, resulting in reduction of foaming capacities. Microstructure determination showed that trehalose attached to the surface of foam membrane; meanwhile, the foaming structure of the complex with 0.5 mol L-1 trehalose had a thicker liquid film with decreased drainage rate, less agglomeration and disproportionation of foam, illustrating the best foaming ability and foaming stability. CONCLUSION: The results suggested that trehalose at different concentrations can interact with MG-11S through different mechanisms, and improve the foaming capacity of MS-11S. This provided a reference for the application of MS-11S in foaming food. © 2022 Society of Chemical Industry.

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