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1.
Artif Intell Med ; 103: 101786, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32143793

RESUMO

As one of the most common neurobehavioral diseases in school-age children, Attention Deficit Hyperactivity Disorder (ADHD) has been increasingly studied in recent years. But it is still a challenge problem to accurately identify ADHD patients from healthy persons. To address this issue, we propose a dual subspace classification algorithm by using individual resting-state Functional Connectivity (FC). In detail, two subspaces respectively containing ADHD and healthy control features, called as dual subspaces, are learned with several subspace measures, wherein a modified graph embedding measure is employed to enhance the intra-class relationship of these features. Therefore, given a subject (used as test data) with its FCs, the basic classification principle is to compare its projected component energy of FCs on each subspace and then predict the ADHD or control label according to the subspace with larger energy. However, this principle in practice works with low efficiency, since the dual subspaces are unstably obtained from ADHD databases of small size. Thereby, we present an ADHD classification framework by a binary hypothesis testing of test data. Here, the FCs of test data with its ADHD or control label hypothesis are employed in the discriminative FC selection of training data to promote the stability of dual subspaces. For each hypothesis, the dual subspaces are learned from the selected FCs of training data. The total projected energy of these FCs is also calculated on the subspaces. Sequentially, the energy comparison is carried out under the binary hypotheses. The ADHD or control label is finally predicted for test data with the hypothesis of larger total energy. In the experiments on ADHD-200 dataset, our method achieves a significant classification performance compared with several state-of-the-art machine learning and deep learning methods, where our accuracy is about 90 % for most of ADHD databases in the leave-one-out cross-validation test.

2.
Development ; 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32165491

RESUMO

The WUSCHEL-CLAVATA3 pathway genes play essential role in shoot apical meristem maintenance and floral organ development, and under intense selection during crop domestication. The carpel number is an important fruit trait affecting fruit shape, size, and internal quality in cucumber, but the molecular mechanism remains elusive. Here, we found that CsCLV3 expression was negatively correlated with carpel number in cucumber cultivars. CsCLV3-RNAi led to increased number of petals and carpels, whereas overexpression of CsWUS resulted in more sepals, petals and carpels, suggesting that CsCLV3 and CsWUS function as a negative and a positive regulator for carpel number variation, respectively. Biochemical analyses indicated that CsWUS directly binds to the promoter of CsCLV3 and activates its expression. Overexpression of CsFUL1 A , a FRUITFULL-like MADS-box gene, resulted in more petals and carpels. CsFUL1A can directly bind to CsWUS promoter to stimulate its expression. Further, we found that auxin participates in carpel number variation in cucumber through interaction of CsARF14 with CsWUS. Therefore, we identified a gene regulatory pathway involving CsCLV3, CsWUS, CsFUL1 A and CsARF14 in determining carpel number variation in an important vegetable crop-cucumber.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32149648

RESUMO

Common spatial pattern (CSP) is an efficient algorithm widely used in feature extraction of EEG-based motor imagery classification. Traditional CSP depends only on spatial filtering, that aims to maximize or minimize the ratio of variances of filtered EEG signals in different classes. Recent advances of CSP approaches show that temporal filtering is also preferable to extract discriminative features. In view of this perspective, a novel spatio-temporal filtering strategy is proposed in this paper. To improve computational efficiency and alleviate the overfitting issue frequently encountered in the case of small sample size, the same temporal filter is designed by EEG signals of the same class and shared by all the spatial channels. Spatial and temporal filters can be updated alternatively in practice. Furthermore, each of the resulting designs can still be cast as a CSP problem and tackled efficiently by the eigenvalue decomposition. To alleviate the adverse effects of outliers or noisy EEG channels, sparse spatial or temporal filters can also be achieved by incorporating an ℓ1-norm-based regularization term in our CSP problem. The regularized spatial or temporal filter design is iteratively reformulated as a CSP problem via the reweighting technique. Two sets of motor imagery EEG data of BCI competitions are used in our experiments to verify the effectiveness of the proposed algorithm.

4.
J Appl Meas ; 21(1): 101-108, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32129772

RESUMO

The estimates of intraclass correlations are known to be biased, but there are few analytical ways to assess the amount of bias. The analytical approach requires the normality assumption to estimate bias. Bootstrap requires no such assumption and can, therefore, be used to estimate bias, regardless of the model assumption. We utilize cluster bootstrapping to calculate the bias in estimating the intraclass correlation. A well-known dataset is provided to illustrate the bias estimation in a typical study design of intraclass correlation, and its implications for other study designs are also discussed.


Assuntos
Modelos Logísticos , Projetos de Pesquisa , Viés , Psicometria
5.
Artigo em Inglês | MEDLINE | ID: mdl-32147667

RESUMO

The specific GluN2B antagonist rislenemdaz (Ris; a.k.a. MK-0657 and CERC-301) is in phase II clinical trial as an antidepressive drug, but the working mechanism for its antidepressant effects is not clearly understood. Given the important role of the lateral habenula (LHb) in the pathogenesis of depression and the fact that GluN2B-containing N-methyl-D-aspartate receptors and brain-derived neurotrophic factor (BDNF) are expressed in the LHb, we conducted a study to examine whether the LHb mediates Ris' antidepressant effects in a chronic restraint stress (CRS)-induced depressive-like mouse model. In this study, Ris was administered systemically or locally into the LHb. Short hairpin RNAs were used to knockdown BDNF in the LHb. Depressive-like behaviors were assessed with the open field test, forced swimming test, tail suspension test, and sucrose preference test. Expression of GluN2B, BDNF, and c-Fos in the LHb were analyzed with western blotting and immunohistochemistry under condition with Ris administered systemically or with BDNF knockdown in the LHb. We found that both systemic and intra-LHb administration of Ris alleviated CRS-induced despair-like behavior and that systemic Ris reduced LHb expression of GluN2B, BDNF, and c-Fos (a neuronal activity marker). Specific knockdown of BDNF in the LHb prevented CRS-induced despair-like behavior, while preventing CRS-induced increases in BDNF and c-Fos expression in the LHb. Together these results suggest that Ris may exert its antidepressant effects through affecting the LHb such as downregulating BDNF expression in the LHb.

6.
PLoS Biol ; 18(3): e3000671, 2020 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-32203514

RESUMO

Domesticated crops with high yield and quality are frequently susceptible to pathogen attack, whereas enhancement of disease resistance generally compromises crop yield. The underlying mechanisms of how plant development and disease resistance are coordinately programed remain elusive. Here, we showed that the basic Helix-Loop-Helix (bHLH) transcription factor Cucumis sativus Irregular Vasculature Patterning (CsIVP) was highly expressed in cucumber vascular tissues. Knockdown of CsIVP caused severe vasculature disorganization and abnormal organ morphogenesis. CsIVP directly binds to vascular-related regulators YABBY5 (CsYAB5), BREVIPEDICELLUS (CsBP), and AUXIN/INDOLEACETIC ACIDS4 (CsAUX4) and promotes their expression. Knockdown of CsYAB5 resulted in similar phenotypes as CsIVP-RNA interference (RNAi) plants, including disturbed vascular configuration and abnormal organ morphology. Meanwhile, CsIVP-RNAi plants were more resistant to downy mildew and accumulated more salicylic acid (SA). CsIVP physically interacts with NIM1-INTERACTING1 (CsNIMIN1), a negative regulator in the SA signaling pathway. Thus, CsIVP is a novel vasculature regulator functioning in CsYAB5-mediated organ morphogenesis and SA-mediated downy mildew resistance in cucumber.

7.
Nano Lett ; 20(3): 2157-2162, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32083884

RESUMO

The formation of the Dirac nodal line (DNL) requires intrinsic symmetry that can protect the degeneracy of continuous Dirac points in momentum space. Here, as an alternative approach, we propose an extrinsic symmetry protected DNL. On the basis of symmetry analysis and numerical calculations, we establish a general principle to design the nonsymmorphic symmetry protected 4-fold degenerate DNL against spin-orbit coupling in the nanopatterned 2D electron gas. Furthermore, on the basis of experimental measurements, we demonstrate the approximate realization of our proposal in the Bi/Cu(111) system, in which a highly dispersive DNL is observed at the boundary of the Brillouin zone. We envision that the extrinsic symmetry engineering will greatly enhance the ability for artificially constructing the exotic topological bands in the future.

8.
Med Sci Monit ; 26: e921502, 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-32066649

RESUMO

BACKGROUND Circular RNAs (circRNAs) are key regulators that take part in the carcinogenesis and development of breast cancer. The current study aimed to identify the expression of and explored the function of circRNA-0001283 in breast cancer. MATERIAL AND METHODS Breast cancer tissue samples were tested using high-throughput sequencing to identify the levels of relative genes; and proteins were addressed by using quantitative real-time polymerase chain reaction (qRT-PCR) and western-blot. Cell ability and cell apoptosis were investigated by Cell Counting Kit-8 (CCK-8) and flow cytometry. Invasion was detected by Transwell invasion assay. The identification of target genes was analyzed by dual-luciferase reporter assay. RESULTS Downregulation of circRNA-0001283 expression was observed in breast cancer tissue samples. Ectopic expression of circRNA-0001283 remarkably suppressed cell viability and invasion, and induced apoptosis in breast cancer cells. Furthermore, circRNA-0001283 bound to miR-187 and decreased the expression of miR-187, which resulted in inhibition in cell growth and invasion. Finally, we showed that circRNA-0001283 positively regulated HIPK3 expression by sponging miR-187. CONCLUSIONS The results reveal a new functional circRNA-0001283 in breast cancer and may provide targets for developing novel therapeutic strategies for breast cancer.

9.
Bioresour Technol ; 302: 122876, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32007854

RESUMO

Co-pyrolysis and steam co-gasification of wheat straw (WS) and lignite coal (LC) were studied in a tube furnace between 700 °C and 900 °C. Synergistic effect in co-pyrolysis is not always apparent. However, with the introduction of H2O vapor, synergetic effect is more obvious. Gas volume generated by co-gasification was higher than the prediction in all cases. Meanwhile, temperature played an important role and had a linear relationship with the excess gas volume when it exceeded 800 °C. These findings can be explained by that sufficient H2O vapor could enhance synergy according raising catalytic effect of alkali and alkaline earth metals (AAEMs), promoting free radical generated and increasing reactivity of half-chars. Moreover, co-gasification of WS and LC with several blending ratios were studied at 850 °C. It found H2O vapor could promote free radical formation stronger with higher ratio of WS during co-gasification, thus showing an enhancing effect on the reactivity of WS-derived chars.


Assuntos
Carvão Mineral , Vapor , Biomassa , Carvão Vegetal , Pirólise , Triticum
10.
J Ethnopharmacol ; 254: 112681, 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-32087320

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Antrodia camphorata (A. camphorata) is a rare functional fungus in Taiwan and contains a variety of biologically active ingredients. Antrodin A (AdA) is one of the main active ingredients in the solid-state fermented A. camphorata mycelium. It protects the liver from alcohol damage by improving the antioxidant and anti-inflammatory capacity of the liver and maintaining the stability of the intestinal flora. AIM OF THE STUDY: The aim of this study was to evaluate the hepatoprotective activities of ethyl acetate layer extract (EALE), AdA, and Antroquinonol (Aq) from mycelium of A. camphorata on alcoholic liver injury. MATERIALS AND METHODS: Mice were given with intragastrically vehicle (NC, 2% CMC-Na), alcohol (AL, 12 mL/kg bw), or different A. camphorata samples (EALE, AdA, Aq) at low (100 mg/kg bw) or high (200 mg/kg bw) dosages. The positive control (PC) group was given with silymarin (200 mg/kg bw). Except the NC group, each group of mice was fasted for 4 h after the last treatment and was intragastrically administrated with 50% alcohol (12 mL/kg bw). At the end of experiment, mouse serum was collected and the liver was excised. A portion of the liver was fixed in formalin and used for histopathological analysis, whereas the rest was used for biochemical analysis and real-time PCR analysis. The intestinal flora structure of feces was analyzed by determining the v3-v4 region sequence in 16S rDNA. RESULTS: The high-dose groups of the three samples (EALEH, AdAH, and AqH) significantly alleviated the alcohol-induced increases in liver index, serum ALT, AST, and AKP activities. Serum TG level was significantly reduced in all treatment groups. The increase of HDL-C content indicated that active ingredients of A. camphorata could reduce the lipid content in serum. Furthermore, MDA contents of the AdAH and AqH groups in liver were significantly reduced, accompanying with the levels of SOD, CAT, and GSH elevated to various extents. Antioxidant and anti-inflammatory capabilities in the liver were increased in the AdAH group, as evidenced by the mRNA expression levels of Nrf-2 and HO-1 were significantly increased; while those of CYP2e1, TNF-α, and TLR-4 were significantly decreased. Analysis of intestinal flora of feces showed that alcohol treatment significantly changed the composition of intestinal flora. Supplementation with AdA could mitigate dysbiosis of intestinal flora induced by alcohol. Flora of Faecalibaculum, Lactobacillus, and Coriobacteriaceae_UCG-002 showed significantly negative correlations with ALT, AST, AKP, and MDA levels. CONCLUSION: Antrodin A could improve the antioxidant and anti-inflammatory capacities of the liver and maintain the stability of intestinal flora. It is potentially a good candidate compound against acute alcoholic liver injury.

11.
Nanoscale ; 2020 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-32096537

RESUMO

Electrides possess high electrical conductance and reactivity and are promising for novel applications in electronics and catalysis. Here, we predict a new thermodynamically and kinetically stable two-dimensional (2D) Ca4N2 using first-principles density functional theory (DFT) calculations. 2D Ca4N2 can serve as a one-dimensional (1D) electride [Ca4N2]2+·2e- with anionic electrons confined in the surface channels. In particular, we demonstrate that 2D Ca4N2 possesses high Fermi velocity (0.42 × 106 m s-1), electron effective Fermi mass (∼1me), ultrahigh charge density (1.14 × 1015 cm-2), and high carrier mobility (215 and 5.29 × 106 cm2 V-1 s-1 at a room temperature of 300 K and a low temperature of 2 K), resulting in ultrahigh conductance up to 0.039 and 966 S respectively for 300 and 2 K, compared to existing 2D materials and the best conductors (Cu and Ag). Furthermore, the first finding of 1D anionic electron behaviour on the surface of 2D materials can be used to stimulate the design of new kinds of electrides for exploring the physics of 1D and quasi-1D systems.

12.
Transgenic Res ; 29(1): 149-163, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31927726

RESUMO

Myostatin (MSTN), a member of the transforming growth factor-ß superfamily, is a negative regulator of muscle growth and development. Disruption of the MSTN gene in various mammalian species markedly promotes muscle growth. Previous studies have mainly focused on the disruption of the MSTN peptide coding region in pigs but not on the modification of the signal peptide region. In this study, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) system was used to successfully introduce two mutations (PVD20H and GP19del) in the MSTN signal peptide region of the indigenous Chinese pig breed, Liang Guang Small Spotted pig. Both mutations in signal peptide increased the muscle mass without inhibiting the production of mature MSTN peptide in the cells. Histological analysis revealed that the enhanced muscle mass in MSTN+/PVD20H pig was mainly due to an increase in the number of muscle fibers. The expression of MSTN in the longissimus dorsi muscle of MSTN+/PVD20H and MSTNKO/PVD20H pigs was significantly downregulated, whereas that of myogenic regulatory factors, including MyoD, Myogenin, and Myf-5, was significantly upregulated when compared to those in the longissimus dorsi muscle of wild-type pigs. Meanwhile, the mutations also activated the PI3K/Akt pathway. The results of this study indicated that precise editing of the MSTN signal peptide can enhance porcine muscle development without markedly affecting the expression of mature MSTN peptide, which could exert other beneficial biological functions in the edited pigs.

13.
Anal Chem ; 92(3): 2853-2858, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-31916749

RESUMO

The exhaustive investigating interactions between recognition probes and amyloid aggregates, especially simultaneous recognition events, are challenging and crucial for the design of biosensing probes and further diagnosis of amyloid diseases. In the present work, the interactions of aptamers (Apts) with ß-amyloid (Aß) aggregates were explored thoroughly by single-molecule force spectroscopy (SMFS). Indeed, it was found that the interaction of aptamer1 (Apt1)-amyloid aggregates was different from that of aptamer2 (Apt2)-Aß40 aggregates at the single-molecule level. Especially, the interaction force of Apt1-Aß40 fibril showed a double distinguishing Gaussian fitting. The only unimodal distribution of the force histogram was displayed for the interactions of Apt2-Aß40 oligomer, Apt2-Aß40 fibril, and Apt1-Aß40 oligomer. More intriguingly, two Apts could bind to amyloid aggregates simultaneously. With the assistance of two Apts recognition, a novel sensitive dual Apt-based surface plasmon resonance (SPR) sensor using Au nanoparticles (AuNPs) was developed for quantifying Aß40 aggregates. The dual Apt-based SPR sensor not only avoided the limitation of steric hindrance and epitope but also employed simple operation as well as inexpensive recognition probes. A detection limit as low as 0.2 pM for Aß40 oligomer and 0.05 pM for Aß40 fibril could be achieved. Moreover, the established sensor could be successfully applied to detect Aß40 aggregates in artificial cerebrospinal fluid (CSF) and undiluted real CSF. This work could provide a strategy to monitor a simultaneous recognition event using SMFS and broaden the application of Apts in the diagnosis of neurodegenerative diseases.

14.
Br J Radiol ; 93(1108): 20190154, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31944856

RESUMO

OBJECTIVE: This work focused on the function role and underlying mechanism of BLACAT1 in regulating the radiosensitivity of head and neck squamous cell carcinoma (HNSCC) cells via PSEN1. METHODS: BLACAT1 and PSEN1 expression in HNSCC tissues and cells were measured by qRT-PCR. Kaplan-Meier method and Spearman's correlation analysis determined the prognostic roles and association of BLCAT1 and PSEN1 in HNSCC. The impacts of BLACAT1 and PSEN1, alone and in combination, on radiosensitivity of HNSCC cells were separately assessed through CCK-8, colony formation, flow cytometry, western blot and γH2AX foci staining assays. RESULTS: Our study disclosed that BLACAT1 and PSEN1 were both in association with poor prognosis and radioresistance of HNSCC cells. BLACAT1 knockdown improved the radiosensitivity of HNSCC cells by changing cellular activities containing repressed cell viability, accelerated cell apoptosis, induced cell cycle arrest, and stimulated DNA damage response. Further, we found that PSEN1 was positively correlated with BLACAT1. Rescue assays confirmed that BLACAT1 regulated the radiosensitivity of HNSCC cells by modulating PSEN1. CONCLUSION: We revealed that BLACAT1 knockdown enhanced radioresistance of HNSCC cells via regulating PSEN1, exposing the probable target role of BLACAT1 in HNSCC. ADVANCES IN KNOWLEDGE: This was the first time that the pivotal role of BLACAT1 was investigated in HNSCC, which provided a novel therapeutic direction for HNSCC patients.


Assuntos
Neoplasias de Cabeça e Pescoço/genética , Presenilina-1/metabolismo , RNA Longo não Codificante/genética , Tolerância a Radiação/genética , Carcinoma de Células Escamosas de Cabeça e Pescoço/genética , Apoptose/genética , Ciclo Celular/genética , Linhagem Celular Tumoral , Sobrevivência Celular/genética , Dano ao DNA , Feminino , Expressão Gênica , Técnicas de Silenciamento de Genes , Neoplasias de Cabeça e Pescoço/radioterapia , Humanos , Neoplasias Hipofaríngeas/genética , Neoplasias Hipofaríngeas/radioterapia , Neoplasias Laríngeas/genética , Neoplasias Laríngeas/radioterapia , Masculino , Proteínas de Neoplasias/metabolismo , Neoplasias Orofaríngeas/genética , Neoplasias Orofaríngeas/radioterapia , RNA Longo não Codificante/metabolismo , Carcinoma de Células Escamosas de Cabeça e Pescoço/radioterapia , Regulação para Cima
15.
Food Funct ; 11(1): 270-284, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31957758

RESUMO

Lactobacillus salivarius AR809 is a newly discovered probiotic strain from a healthy human pharynx and has potential ability to adhere to the pharyngeal epithelium and inhibit Staphylococcus aureus (S. aureus)-induced inflammatory response. Pharyngeal spray administration of AR809 exhibited protective effects in a S. aureus-induced mouse model of pharyngitis. The inhibitory effect and underlying molecular mechanism of AR809 on S. aureus-stimulated pharyngitis were further investigated. AR809 significantly increased phagocytosis and bactericidal activity, reduced the production of inflammatory mediators (intracellular reactive oxygen species (ROS), prostaglandin E2 (PGE2), cyclooxygenase-2 (COX-2), nitric oxide (NO), inducible NOS (iNOS)) and the expression of inflammatory cytokines (tumor necrosis factor-α (TNF-α) and interleukin-1ß (IL-1ß)), and induced macrophages to adopt the M2 phenotype. AR809 also attenuated S. aureus-induced phosphorylations of protein kinase B (Akt) and rapamycin (mTOR), and elevated the autophagic protein (light chain 3 from II (LC3-II) and Beclin-1) level. Furthermore, AR809 inhibited nuclear transcription factor kappa-B (NF-κB) activation by suppressing the nuclear translocation of NF-κB p65. Likewise, 740Y-P (a PI3K activator) decreased the anti-inflammatory effect of AR809 against S. aureus-induced inflammatory response, while AR809 treatments with wortmannin (a PI3K inhibitor) markedly reversed this inflammatory response. AR809 prevents S. aureus-induced pharyngeal inflammatory response, possibly by regulating TLR/PI3K/Akt/mTOR signalling pathway-related autophagy and TLR/PI3K/Akt/IκB/NF-κB pathway activity, and therefore has potential for use in preventing pharyngitis and other inflammatory diseases.

16.
ACS Nano ; 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31994861

RESUMO

The high-precision patterning of metal halide perovskites (MHPs) is of paramount importance for their device application. Here, we demonstrate the femtosecond (fs)-laser-assisted formation of three-dimensional MHP nanocrystal (NC) patterns with strong blue photoluminescence (PL) inside an oxide glass. Our strategy enables the crystallization and erasing of CsPb(Cl/Br)3 NCs inside a glass localized around the laser focal area through a combination of fs laser irradiation and thermal treatment processes. These recoverable patterns exhibit a switchable PL associated with the laser-induced defect and the thermal healing of MHP NCs that are benefits from the soft ionic crystal structure and low formation energy of the MHPs. Due to the high stability offered by the protection of the oxide glass matrix, the laser printing of fine-structured MHP micropatterns can be repeated over multiple cycles with a high robustness compared with their colloidal process counterparts. Our results demonstrate a simple strategy for creating emissive patterns inside a stable and transparent solid matrix that could be promising for applications including information storage, three-dimensional displays, anticounterfeit labels, and information security protection.

17.
Inorg Chem ; 59(3): 1577-1581, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-31965797

RESUMO

The discovery of new inorganic functional chalcogenides is of fundamental importance in structure chemistry and materials science. Herein, a new type of pentanary thioantimonates, A2Ba3Cu2Sb2S10 (A = K, Rb, Cs), has been discovered by a solvothermal method at low temperature. These isostructural compounds belong to the monoclinic space group C2/m (No. 12) and comprise unique one-dimensional (1D) [Cu2Sb2S10]8- chains surrounded by discrete A+ and Ba2+ cations. Such a 1D chain structure is the first case of chalcogenides in the X/Cu/Sb/Q (X = cations; Q = chalcogen) system. The energy gaps range from 1.90 to 1.98 eV for A2Ba3Cu2Sb2S10 (A = K, Rb, Cs) were obtained from the UV-visible reflectance spectroscopy. Moreover, Rb2Ba3Cu2Sb2S10 displays an intriguing photocurrent response under simulated solar-light illumination. The theoretical calculations were also carried out to understand the interrelationship of the optical performance and electronic structure.

18.
J Ind Microbiol Biotechnol ; 47(2): 197-207, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31933057

RESUMO

The goals of this study were to increase the production of antroquinonol (AQ) and to elucidate the response mechanism of the cell membrane during the in situ extractive fermentation (ISEF) of Antrodia camphorata S-29. Through ISEF, the concentration of AQ reached a maximum of 146.1 ± 2.8 mg/L, which was approximately (7.4 ± 0.1)-fold that of the control (coenzyme Q0-induced fermentation). Transcriptome sequencing showed that four genes (FAD2, fabG, SCD, and FAS1) related to fatty acid biosynthesis were upregulated. FAD2 and SCD may regulate the increase in oleic acid (C18:1) and linoleic acid (C18:2) in the cell membrane of A. camphorata S-29, resulting in an increase in cell membrane permeability. AQ was successfully transferred to the n-tetradecane phase through the cell membrane, reducing product feedback inhibition and improving the production of AQ from A. camphorata S-29.

19.
Proteomics Clin Appl ; 14(1): e1900016, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31707769

RESUMO

PURPOSE: The authors aimed to separate Fc N-glycopeptides of disease-specific immunoglobulin G (DSIgG) as personalized biomarkers to distinguish non-small cell lung cancer (NSCLC) from benign lung diseases (BLDs). EXPERIMENTAL DESIGN: DSIgG from 509 BLDs patients and 477 NSCLC patients was isolated using native polyacrylamide gel electrophoresis and then the Fc glycosylation was determined using mass spectrometry. RESULTS: For the patients below 60 years of age, a combination of the glycopeptides ratios with one fucose residue difference of DSIgG1 and DSIgG2 can differentiate NSCLC from BLDs, with area under curve (AUC) values of >0.76, sensitivities of >87%, and specificities of >61%. For the patients above 60 years of age, a combination of the glycopeptides ratios with one monosaccharide residue of DSIgG2 can differentiate NSCLC from BLDs, with AUC values of >0.78, sensitivities of >91%, and specificities of >54%. For the same participants, the commonly used clinical biomarkers have AUC values of 0.5-0.621, sensitivities of 15.8-32.9%, and specificities of 75.7-90.5%. CONCLUSIONS: These findings indicate that these DSIgG Fc glycoforms are potential personalized biomarkers to differentiate NSCLC from BLDs.

20.
Waste Manag ; 102: 190-197, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31678805

RESUMO

In the process of landfilling, leachates resulting from the waste landfill are likely to cause secondary environmental pollution, and installation of a basal liner is essential under a landfill site to block and reduce permeation of leachate flowing into the subsurface environment. The research aims to develop a salt-resistant bentonite and a novel base liner material for offshore waste disposal. The liner materials consist of core materials and coating materials in which mixtures of bentonite, sepiolite, and guar gum were used to overcome the shortcomings in bentonite to realise a high water-resistance and a permeability coefficient of below 1.0 × 10-7 cm/s under saline water conditions. The optimal mixing ratio of bentonite, sepiolite, and guar gum was confirmed as 76:19:5 by conducting drying shrinkage cracking tests, free swelling tests, and hydraulic conductivity tests. The hydraulic conductivities of spherical particles, as measured in a rigid-wall permeameter and a flexible-wall permeameter, were less than 1.0 × 10-7 cm/s under saline water conditions. The compressive properties of spherical particles were evaluated through triaxial compression testing. The engineering characteristics of the liner material were studied in the present research, but the long-term biodegradation characteristics of polymer additives were also important, yet remained unclear. The long-term biostability of the additives, and its effect on basal liner performance should be evaluated in future research.


Assuntos
Eliminação de Resíduos , Instalações de Eliminação de Resíduos , Bentonita
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