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1.
Sci Adv ; 6(1): eaaw8113, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31922001

RESUMO

P-rich transition metal phosphides (TMPs) with abundant P sites have been predicted to be more favorable for hydrogen evolution reaction (HER) catalysis. However, the actual activities of P-rich TMPs do not behave as expected, and the underlying essence especially at the atomic level is also ambiguous. Our structural analysis reveals the inferior activity could stem from the reduced overlap of atomic wave functions between metal and P with the increase in P contents, which consequently results in too strong P-H interaction. To this end, we used N-induced lattice contraction to generally boost the HER catalysis of P-rich TMPs including CoP2, FeP2, NiP2, and MoP2. Refined structural characterization and theoretical analysis indicate the N-P strong interaction could increase the atomic wave function overlap and eventually modulate the H adsorption strength. The concept of lattice engineering offers a new vision for tuning the catalytic activities of P-rich TMPs and beyond.

2.
Insect Sci ; 27(1): 2-13, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29943911

RESUMO

The silkworm Bombyx mori is an important lepidopteran model insect in which many kinds of natural mutants have been identified. However, molecular mechanisms of most of these mutants remain to be explored. Here we report the identification of a gene Bm-app is responsible for the silkworm minute wing (mw) mutation which exhibits exceedingly small wings during pupal and adult stages. Compared with the wild type silkworm, relative messenger RNA expression of Bm-app is significantly decreased in the u11 mutant strain which shows mw phenotype. A 10 bp insertion in the putative promoter region of the Bm-app gene in mw mutant strain was identified and the dual luciferase assay revealed that this insertion decreased Bm-app promoter activity. Furthermore, clustered regularly interspaced short palindromic repeats/RNA-guided Cas9 nucleases-mediated depletion of the Bm-app induced similar wing defects which appeared in the mw mutant, demonstrating that Bm-app controls wing development in B. mori. Bm-app encodes a palmitoyltransferase and is responsible for the palmitoylation of selected cytoplasmic proteins, indicating that it is required for cell mitosis and growth during wing development. We also discuss the possibility that Bm-app regulates wing development through the Hippo signaling pathway in B. mori.


Assuntos
Aciltransferases/genética , Bombyx/genética , Proteínas de Insetos/genética , Asas de Animais/crescimento & desenvolvimento , Aciltransferases/metabolismo , Animais , Bombyx/crescimento & desenvolvimento , Proteínas de Insetos/metabolismo , Pupa/genética , Pupa/crescimento & desenvolvimento
3.
Arch Oral Biol ; 109: 104579, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31634727

RESUMO

OBJECTIVES: To investigate the effect and mechanism of calcium on LS8 cell differentiation, especially on phosphatidylinositol 3 kinase (PI3K) /protein kinase B(AKT) pathway. MATERIALS AND METHODS: Ameloblast-like LS8 cell line was used and additional 0-3.5 mmol/L calcium chloride was treated for 24 h, 48 h. Cell viability and morphological changes, cell cycle and associated regulatory proteins were analyzed. RESULTS: No significant effects on morphological changes were observed. Decreased cell viability and increased S phase cells were accompanied by the significant decrease of cyclin A and cyclin B proteins, and significant increase of cyclin D protein in LS8 cells. Additionally, kallikrein-4 and amelotin expressions were significantly increased. Finally, the levels of PI3K, AKT, p-AKT and forkhead box O3 (FOXO3) significantly downregulated after calcium treatment in LS8 cells. CONCLUSIONS: Calcium inhibit proliferation and promotes differentiation in LS8 cells, this is closely related to the downregulation of PI3K/AKT signal in LS8 cells.


Assuntos
Ameloblastos/enzimologia , Cálcio/farmacologia , Fosfatidilinositol 3-Quinase/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais , Ameloblastos/efeitos dos fármacos , Animais , Diferenciação Celular , Linhagem Celular , Proliferação de Células , Regulação para Baixo , Camundongos
4.
Sci Total Environ ; : 134563, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31812424

RESUMO

Soil respiration (Rs) in response to climate warming received a wide concern due to its important role in terrestrial ecosystem carbon (C) cycling, but the warming-induced effects of soil microbes on soil respiration are still less understood, especially over time. Our study aims to understand the long-term warming induced effects of soil microbes on Rs. A field soil warming experiment using a completely randomized design was conducted in a naturally regenerated oak forest (Quercus aliena) in central China. Soil warming was executed by infrared heater throughout the period from 2011 to 2015. Our results showed that soil temperature was a main factor in regulating Rs in a temperate oak forest throughout the experiment, while soil water content determined Rs only when a naturally dry year occurred. The positive effect of soil warming on Rs that was observed (i.e., 37.5 to 42.0% in the first two years) gradually diminished in the following three years (i.e., 0.9 to 15.4%). Significant positive warming effects on the temperature sensitivity of Rs (Q10) only occurred in the second year. Continuous soil warming caused the decline in nitrogen (N) availability, with a significant increase in microbial biomass-specific enzyme activities for N-acquisition. The attenuation of microbial biomass increment and the decreased ratio of enzymatic C:N acquisition contributed to the diminished warming effect on Rs over time. Our study suggests that microbe-mediated attenuation of Rs, accompanied by the concomitant decline in soil N availability in response to warming, should be taken into consideration in global C cycle modeling.

5.
Sci Total Environ ; : 135128, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31806337

RESUMO

With the rapid economic growth and a large number of industrial production activities in China in recent years, the public has paid more and more attention to the improvement of environmental quality. Issues such as control of pollution in water and air resources, solid waste and noise, as well as energy use and alternative energy development, have received increasing attention. How to effectively manage and utilize precious water resources is a major issue for China to achieve sustainable development. The present study tried to the concepts of Data Envelopment Analysis (DEA) with slacks-based model (SBM) model under Constant Returns-to-Scale (CRS) technology (CRS-SBM-DEA) to find a solution, by estimating the total factor water usage efficiencies and sewage treatment efficiencies across Eastern, Central and Western areas in China. The results showed that, before 2007, the Eastern Area in China had the highest water usage efficiency, followed by the West Area and then the Central Area. After 2007, the Western area has gradually become the one with the highest water usage efficiency, followed by the East Area and then the Central Area. In addition, the empirical estimates also consider the impact of the social level. Through panel analysis, this paper finds that population pressure will significantly reduce the efficiency of water use in the region, and the government's actions can effectively boost the use and conservation of water resources.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31838093

RESUMO

PURPOSE: Shifting of the flap position after condylar reconstruction with free fibular flaps is known to occur, but its long-term effects on postoperative esthetic outcomes have not been sufficiently reported. Therefore, in this study, we evaluated the long-term morphologic stability of the free fibular flap neocondyle. PATIENTS AND METHODS: This was a retrospective cohort study. The primary outcome variables were neocondyle regeneration and neocondyle position including the distance between the glenoid fossa and the initial neocondyle (Fo-Co), the distance between the glenoid fossa and the stable neocondyle (Fo-Co'), and shifting of the neocondyle (defined as the distance between the stable neocondyle and the initial neocondyle). The primary predictor variable was time. The other variables were age, gender, diagnosis, and number of fibular segments. Correlation analysis between the predictor variables and outcome variables was performed. RESULTS: The sample was composed of 26 patients (11 male and 15 female patients) with a mean age of 31 years. Diagnosis and number of fibular segments were significantly associated with Fo-Co and Fo-Co' (P < .05). Among the 26 patients, only 11 showed neocondyle regeneration at follow-up (group A) whereas 15 did not (group B). Neocondyle regeneration was significantly associated with patient age (P < .01). Stable Fo-Co and stable time were significantly associated with neocondyle regeneration (P < .05). The mean stable time was significantly shorter in group A (3.64 ± 1.12 months) than in group B (6.67 ± 3.85 months) (P < .05), and the mean Fo-Co' was significantly shorter in group A (13.65 ± 3.94 mm) than in group B (20.68 ± 8.87 mm) (P < .05). CONCLUSIONS: The possibility of neocondyle regeneration is higher in pediatric patients than in adults. Neocondyle regeneration could result in the movement of the neocondyle toward the glenoid fossa with a shorter stable time, which could improve neocondyle repositioning. Repositioning of the neocondyle with free fibular flaps for mandibular condyle defects is a self-adaption process for temporomandibular joint function.

7.
Biomed Res Int ; 2019: 4327046, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31828103

RESUMO

Objectives: To investigate the root morphology and root canal anatomy of maxillary first premolar using microscopic computed tomography (micro-CT). Methods: 324 maxillary first premolars were collected and scanned. The root and canal diameter, canal wall thickness, root taper, and cross-sectional shapes were determined in the single root with 1 canal (SR1C), single root with 2 canals (SR2C), and 2 roots with 2 canals (2R2C) by micro-CT. Results: The results showed that single-rooted maxillary premolars were more common than other types. The incidence of SR1C, SR2C, and 2R2C reached 25%, 26.39%, and 26.39%, respectively. Root and canal diameters and canal wall thickness were decreased from coronal third to apical foramen. The three parameters and canal taper showed increases from buccal and palatal (BP) to mesiodistal (MD) aspects. The root canal tapers were smallest of the middle third level. The findings showed the different variations in 2R2C teeth. The root canal cross-sectional morphology in maxillary first premolars is complicated, including round, oval, long oval, flat canal, and irregular canal shapes. The distribution varied in different aspects. Conclusion: Root canal morphology showed a wide variation and complicated structure. The single-rooted teeth were more common in the Chinese adolescent population, and the majority of maxillary first premolars have two canals.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31880025

RESUMO

Lithium metal is an ideal electrode material for future rechargeable lithium metal batteries. However, the widespread deployment of metallic lithium anode is significantly hindered by its dendritic growth and low Coulombic efficiency, especially in ester solvents. Herein, by rationally manipulating the electrolyte solvation structure with a high donor number solvent, it is successfully demonstrated to enhance the solubility of lithium nitrate in an ester-based electrolyte which enables high voltage lithium metal batteries. Remarkably, the electrolyte with high concentration of LiNO 3 additive presents an excellent Coulombic efficiency up to 98.8% during stable galvanostatic lithium plating/stripping cycles. A full-cell lithium metal battery with a lithium nickel manganese cobalt oxide cathode exhibits a stable cycling performance showing limit capacity decay. This approach provides an effective electrolyte manipulation strategy to develop high voltage lithium metal batteries.

9.
BMC Genomics ; 20(1): 863, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31729950

RESUMO

BACKGROUND: Intramuscular fat (IMF) is one of the most important factors positively associated with meat quality. Triglycerides (TGs), as the main component of IMF, play an essential role in muscle lipid metabolism. This transcriptome analysis of pectoralis muscle tissue aimed to identify functional genes and biological pathways likely contributing to the extreme differences in the TG content of broiler chickens. RESULTS: The study included Jingxing-Huang broilers that were significantly different in TG content (5.81 mg/g and 2.26 mg/g, p < 0.01) and deposition of cholesterol also showed the same trend. This RNA sequencing analysis was performed on pectoralis muscle samples from the higher TG content group (HTG) and the lower TG content group (LTG) chickens. A total of 1200 differentially expressed genes (DEGs) were identified between two groups, of which 59 DEGs were related to TG and steroid metabolism. The HTG chickens overexpressed numerous genes related to adipogenesis and lipogenesis in pectoralis muscle tissue, including the key genes ADIPOQ, CD36, FABP4, FABP5, LPL, SCD, PLIN1, CIDEC and PPARG, as well as genes related to steroid biosynthesis (DHCR24, LSS, MSMO1, NSDHL and CH25H). Additionally, key pathways related to lipid storage and metabolism (the steroid biosynthesis and peroxisome proliferator activated receptor (PPAR) signaling pathway) may be the key pathways regulating differential lipid deposition between HTG group and LTG group. CONCLUSIONS: This study showed that increased TG deposition accompanying an increase in steroid synthesis in pectoralis muscle tissue. Our findings of changes in gene expression of steroid biosynthesis and PPAR signaling pathway in HTG and LTG chickens provide insight into genetic mechanisms involved in different lipid deposition patterns in pectoralis muscle tissue.

10.
Sci Total Environ ; : 134657, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31753495

RESUMO

China has established the River Chief System (RCS) to develop mechanisms to achieve success in cross-regional negotiations of the Sustainable Water Resource Management Affairs (SWRMA). This paper aims to investigate the mechanisms developed by RCS and social welfare brought by RCS in China, and thereafter put forward suggestions for the future development of RCS. Therefore, a one-stage cooperation game was used to investigate cross-regional SWRMA negotiations with RCS. The results showed that RCS ensures that cooperative solutions are chosen in cross-regional SWRMA negotiations. Moreover, this paper used a two-stage game to explore cross-regional SWRMA negotiations without involving RCS. In this case, the involvement of the upper-level administrators and the probability of a successful negotiation are endogenously determined by local authorities. The results showed that in cross-regional SWRMA negotiations, both when the upper-level administrators are involved in the first stage and in the second stage, the cooperative solutions would be adopted. Thus, RCS is an inevitable outcome of the cross-regional SWRMA negotiations. In addition, RCS could help to avoid transactional costs and external costs in cross-regional SWRMA negotiations. Based on these results, this study provided the following suggestions: the river chief and local authorities of RCS should be stakeholders; the major members of RCS should be the local authorities influenced mostly by SWRMA quantities; RCS should actively advertise the benefits and the cost-down of SWRMA.

11.
Int J Ophthalmol ; 12(11): 1801-1806, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31741872

RESUMO

This study investigated the potential role of MAPK signaling pathways in conjunctivochalasis (CCH). Twenty loose conjunctival biopsy samples from 20 CCH and 15 conjunctival biopsy samples from 15 normal controls (CON) were collected. The conjunctival fibroblasts were cultured in vitro. Immunofluorescence, ELISA, Western blot and reverse transcription-polymerase chain reaction (RT-PCR) were used. Our results showed that the expression of p-ERK, p-JNK, and p-p38 in CCH conjunctiva was significantly higher than that in CON group. The expression of p38 MAPK, JNK, and ERK proteins in CCH fibroblasts was significantly higher than that in CON group. The total expression of MAPK mRNA in CCH fibroblasts was significantly higher than that in CON group. The activated forms of p38 MAPK, JNK, and ERK proteins and mRNAs might up-regulate the expression of MMPs in CCH loose conjunctival tissue and fibroblasts, causing the degradation of collagen fibers and elastic fibers and promoting the occurrence of CCH. Our results deepen the understanding of CCH pathological mechanism.

12.
Placenta ; 89: 33-41, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31675488

RESUMO

INTRODUCTION: Embryo implantation depends on trophoblast cells migration and invasion. Abnormal function of trophoblast cells could result in many pregnancy complications. Secreted protein acidic and rich in cysteine like-1 (SPARCL1) has been reported to inhibit cell migration and tumor invasion. This study aimed to explore the role of SPARCL1 in trophoblast functions. METHODS: Villous specimens were obtained from 31 women with spontaneous abortion and 31 women with normal early pregnancy to determine the expression of SPARCL1. HTR8/SVneo cells and JAR cells were transfected with pIRES2-EGFP-SPARCL1 vectors and control vectors. The proliferation assay and scratch-wound assay were performed. Quantitative polymerase chain reaction (qPCR) and western blotting were performed to assess epithelial mesenchymal transition (EMT)-related molecules including MMP2, MMP3, N-cadherin, E-cadherin and vimentin. Extracellular signal-regulated kinase (ERK) phosphorylation activity and AP-1 expression in HTR8/SVneo cells following multi-scratching were detected using above assays. RESULTS: The mRNA and protein levels of SPARCL1 were significantly higher in the abortion group than in the normal pregnancy group. After transfection, there was no difference of cell viability between the SPARCL1-overexpression group and control vector group. However, the migration distance and area were reduced and the abundances of EMT related molecules were changed by SPARCL1 overexpression when compared with controls. Lower ERK phosphorylation activity and decreased Fos and Jun expressions were noted at high level of SPARCL1. CONCLUSION: Restrained migration and invasion were noted in trophoblast cells with SPARCL1 overexpression, which might affect embryo implantation and placenta development. It could be involved in the pathogenesis of spontaneous abortion.

13.
Int Immunopharmacol ; 77: 105971, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31678865

RESUMO

Estrogen receptor (ER) ß activation has anti-inflammatory activity. However, its effect on the development of inflammatory bowel disease (IBD) and the underlying mechanism have not been clarified. This study aimed to assess the clinical value of ERß+CD4+ T cells in IBD patients and examine the anti-inflammatory role of ERß activation in dextran sulfate sodium (DSS)-induced chronic colitis in mice. We investigated the effects of ERB041 (an ERß-specific agonist) on inflammatory cytokines and pro-inflammatory T-cell and regulatory T-cell (Treg) responses in murine colitis. We tested the role of ERß activation on Treg differentiation and its activity to suppress T-cell proliferation in vitro. We found that reduced frequency of circulating ERß+CD4+ T cells in IBD patients was negatively correlated with inflammation and disease severity. ERß and FoxP3 expression co-localized in the intestinal tissues of IBD patients. Treatment with ERB041 significantly mitigated colitis-induced weight loss, inflammation, and disease severity. It also restored the ERß+CD4+ T cell population in the spleen and colon lamina propria of these mice. ERB041 treatment inhibited CD4+CD25- and CD8+ T cell infiltration and restored Tregs and activated T-cell immunoreceptor with Ig and ITIM domains (TIGIT)+ Tregs in the colon lamina propria. In vitro, we found that ERß activation enhanced Treg differentiation, immunosuppression, and TGF-ß1/Smad signaling in CD4+ T cells. Our data suggest that ERß+CD4+ T cells represent a potential biomarker for evaluating IBD disease severity, and ERß activation may be valuable for the treatment of IBD by enhancing the Treg response.

14.
Ying Yong Sheng Tai Xue Bao ; 30(10): 3452-3462, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31621232

RESUMO

To explore light energy utilization characteristics and yield effect of different legume-gramineae intercropping patterns, we set up five kinds of monocropping patterns including alfalfa, triticale, oats, maize and sorgo as reference in a field experiment. The light energy utilization chara-cteristics and productivity of four kinds of intercropping patterns including alfalfa-triticale, alfalfa-oats, alfalfa-maize and alfalfa-sorgo were examined. The contribution degree of each light energy index to yield formation was analzyed using path analysis method. The results showed that the contribution of each index of light energy to yield from large to small was as follows: leaf area index (LAI)=1.236, net photosynthetic rate (Pn)=0.519, canopy openness (DIFN)=0.302, intercellular CO2 concentration (Ci)=-0.026, stomatal conductance (gs)=-0.116, transpiration rate (Tr)=-0.188, PAR interception rate (FIPAR)=-1.708. Light use efficiency (LUE) as a comprehensive indicator of light energy utilization had the largest value (1.367). Compared with monoculture, the values of LAI, Pn, gs, Tr and FIPAR of four kinds of gramineae grasses increased under intercropping and the values of DIFN and Ci decreased, while alfalfa showed an opposite trend. Compared with monoculture, the LUE of four kinds of gramineae grasses under intercropping was significantly increased. The increase rates of triticale and oats (35.2% and 30.4%) were higher than that of maize and sorgo (28.7% and 26.3%). The decrease rates of alfalfa intercropping with triticale and oats (6.1% and 8.3%) were obviously lower than that of maize and sorgo (21.8% and 24.5%). The values of land equivalent ratio (LER) of four kinds of intercropping patterns was all greater than 1. The LER values of alfalfa-triticale and alfalfa-oat were significantly higher than those of alfalfa-maize and alfalfa-sorgo. It could be seen that LAI had the largest direct contribution to yield, followed by Pn. Among the four intercropping patterns, two patterns, alfalfa-triticale and alfalfa-oats, had greater improvement potential of light energy utilization and yield.


Assuntos
Agricultura , Produtos Agrícolas , Medicago sativa , Fotossíntese , Zea mays
15.
Artigo em Inglês | MEDLINE | ID: mdl-31605678

RESUMO

PURPOSE: Dynamic asymmetry has not been as rigorously evaluated as static asymmetry for patients with skeletal deformity but could well be even more important. The aim of the present study was to evaluate the dynamic facial movement of Class III patients with facial asymmetry using a 3-dimensional (3D) motion capture system. MATERIALS AND METHODS: The present cross-sectional study recruited patients with skeletal Class III malocclusion with and without facial asymmetry. A 3D facial motion capture system was used to record the expression process of a maximal smile. Eleven orofacial landmarks were selected to analyze and calculate the cumulative distance and average speed of each landmark during smiling. The predictor variable was mandibular symmetry. The outcome variables consisted of the measurements of each soft tissue landmark and the absolute differences for the paired landmarks between 2 sides. Other variables consisted of descriptive data, including the age and gender of each patient. The data were analyzed using independent t tests and paired t tests. Bonferroni's adjustment was used to control for multiple comparisons. RESULTS: A total of 63 patients were divided into 2 groups, an asymmetric group (n = 46) and a control group (n = 17), according to the degree of skeletal deviation. The difference in the cumulative distance of the bilateral cheilions was statistically significant between the 2 groups (P = .002). The difference for the asymmetric and control groups was 2.06 ± 1.78 mm and 1.00 ± 0.79 mm, respectively. In the asymmetric group, a comparison of the deviated side with the nondeviated side revealed statistically significant differences in the magnitude of motion for the cheilion (P < .01) and midlateral lower lip (P < .01). CONCLUSIONS: The patients with skeletal asymmetry also showed asymmetry in soft tissue functions while smiling. The magnitude of movement in the nondeviated side was greater than that in the deviated side.

16.
Medicine (Baltimore) ; 98(39): e17296, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31574852

RESUMO

The angiotensin-receptor-neprilysin inhibitor (ARNI) reduced cardiovascular deaths and heart failure hospitalization in patients with heart failure of reduced ejection fraction (HFrEF). Its role in non-HFrEF patients was not clear. This study aims to answer this question.In this retrospective study, we enrolled 928 patients diagnosed with non-HFrEF, 492 of them received angiotensin converting enzyme inhibitor (ACEI) and the rest 436 received angiotensin-receptor-neprilysin inhibitor. Outcomes were compared by Kaplan-Meier survival analysis and various clinical parameters were investigated using Cox multivariable analysis, followed by interaction analysis. Minnesota living with heart failure Questionnaire (MLHFQ) was employed as one of the criteria to assess heart failure outcome.The cardiovascular (CV) death or HF hospitalization at 24 months occurred in 49 patients in ACEI group compared with 31 in ARNI group (Hazard Ratio (HR): 1.231, 95% confidence Interval (CI): 1.080-2.460, P = .031). And ARNI showed better prognosis of HF hospitalization (HR: 1.283, 95%CI: 1.065-1.360, P = .038). Cumulative Kaplan-Meier estimates of endpoints, ARNI could reduce the incidence of CV death or HF hospitalization (P = .042) and HF hospitalization (P = .035). The stratified analysis revealed that participants with age less than 70 years old had a lower incidence of CV death or HF hospitalization (HR: 1.194, 95%CI: 1.011-1992, P = .031) after treated with ARNI. Patients received diuretics could benefit from ARNI (HR: 1.383, 95%CI: 1.082-1.471, P = .019). Similar results were also observed in patients with heart rate lower than 90 bpm (HR: 1.556, 95%CI: 1.045-2.386, P = .003) and patients with atrial fibrillation history (HR: 1.873, 95%CI: 1.420-2.809, P = .011). ARNI could improve the quality of life both from the total, emotional and physical aspects.ARNI is an efficacy treatment strategy to improve the outcome and quality of life in patients with non-HFrEF.


Assuntos
Antagonistas de Receptores de Angiotensina , Insuficiência Cardíaca , Hospitalização/estatística & dados numéricos , Neprilisina/antagonistas & inibidores , Qualidade de Vida , Volume Sistólico , Idoso , Antagonistas de Receptores de Angiotensina/administração & dosagem , Antagonistas de Receptores de Angiotensina/efeitos adversos , China/epidemiologia , Feminino , Insuficiência Cardíaca/tratamento farmacológico , Insuficiência Cardíaca/mortalidade , Insuficiência Cardíaca/fisiopatologia , Insuficiência Cardíaca/psicologia , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Resultado do Tratamento , Função Ventricular Esquerda
17.
Medicine (Baltimore) ; 98(40): e17449, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31577770

RESUMO

BACKGROUND: Pulmonary artery sling (PAS) is rare, often with tracheal stenosis. And the postoperative mortality is high. For now, there is no consensus on the tracheoplasty for the patients with PAS and tracheal stenosis. METHODS: Studies involving surgical repair of PAS and tracheal stenosis with and without tracheoplasty were identified by searching the PubMed, EMBASE, and the Cochrane Library databases until June 5, 2019. The assessed variables included ventilation time, early and late mortality, and symptom at follow-up. A random-effect/fixed-effect model was used to summarize the estimates of the mean difference (MD)/risk ratio (RR) with 95% confidence interval (CI). RESULTS: This study will be submitted to a peer-reviewed journal for publication. CONCLUSION: This study will assess the safety and efficacy of tracheoplasty for patients with PAS and tracheal stenosis, and provide more evidence-based guidance in clinical practice. PROSPERO REGISTRATION NUMBER: CRD42019139788.


Assuntos
Cardiopatias Congênitas/complicações , Cardiopatias Congênitas/cirurgia , Artéria Pulmonar/anormalidades , Procedimentos Cirúrgicos Reconstrutivos , Estenose Traqueal/complicações , Estenose Traqueal/cirurgia , Humanos
18.
J Immunol ; 203(10): 2571-2576, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31597706

RESUMO

Neonates often develop poor immunity against intracellular pathogens. Because CD8+ T cells are essential for eliminating infectious agents, it is crucial to understand why they behave differently in early life. Previous studies in mice have demonstrated that neonatal CD8+ T cells fail to form memory because of an intrinsic propensity to differentiate into short-lived effectors. However, the underlying mechanisms remain undefined. We now show that neonatal CD8+ T cells exhibit higher glycolytic activity than adult CD8+ T cells postinfection, which may be due to age-related differences in Lin28b expression. Importantly, when glycolysis is pharmacologically inhibited, the impaired formation of neonatal memory CD8+ T cells can be restored. Collectively, these data suggest that neonatal CD8+ T cells are inherently biased toward undergoing glycolytic metabolism postinfection, which compromises their ability to develop into memory CD8+ T cells in early life.

19.
J Am Chem Soc ; 141(45): 18013-18020, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31626550

RESUMO

Cell-cell interactions are mediated through compositions expressed on the membrane. Engineering the cell surface to display functional modules with high biocompatibility, high controllability, and high stability would offer great opportunities for studying and manipulating these intercellular reactions. However, it remains a technical challenge because of the complex and dynamic nature of the cell membrane. Herein, by using three-dimensional (3D) amphiphilic pyramidal DNA as the scaffold, we develop a biocompatible, effective, and versatile strategy for engineering the cell surface with DNA probes. Compared with linear DNA constructs, these pyramidal probes show higher (nearly 100-fold) membrane-anchoring stability and higher (about 2.5-fold) target accessibility. They enable specific, effective, and tunable connections between cells. Meanwhile, our results indicate that connecting cells in close proximity are critical to initiate intercellular communication. By combining high programmability and high diversity of DNA probes, this strategy is expected to provide a powerful and designable membrane-anchored nanoplatform for studying multicellular communication networks.

20.
Chemistry ; 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31644833

RESUMO

Aggregation-induced emission (AIE) materials have drawn great attention for applications as organic light-emitting diodes (OLED) and probes. The applications are, however, restricted by the complex syntheses and hydrophobic properties. Herein, a one-step synthesis of an AIE material based on imidazole hydrazone is assessed. Protonation of the imidazole-H leads to emission color change from yellow to green in the solid state. The emission color is recovered upon imidazole-H+ deprotonation. Moreover, the emission wavelength shifts from 532 to 572 nm by anion exchange. In addition, an enhanced emission (ΦF up to 22.6 %) was obtained with the Br- anion compared with NTf2 - , SbCl5 - , PF6 - , and OTf- anions. X-ray crystallography studies together with theoretical calculations show that the enhanced emission of hydrazone salts arises from strong hydrogen bonding between the hydrazone proton and the halide ion (Cl- or Br- ).

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