Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 599
Filtrar
1.
Anal Chim Acta ; 1189: 339223, 2022 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-34815054

RESUMO

The rapid detection of the pathogenic bacteria in patient samples is crucial to expedient patient care. The proposed approach reports the development of a novel lab-on-a-chip device for the rapid detection of P. aeruginosa based on immunomagnetic separation, optical scattering, and machine learning. The immunomagnetic particles with a diameter of 5 µm were synthesized for isolating P. aeruginosa from the test sample. A microfluidic chip was fabricated, and three optical fibers were embedded for connecting a laser light and two photodetectors. The laser light was pointed towards the channel to pass light through the sample. A pair of photodetectors via optical fibers were arranged symmetrically at 45° to the channel. The photodetectors acquired scattered light from the flowing sample and converted the light to an electrical signal. The sample containing immunomagnetic beads linked with bacteria was injected into the microfluidic chip. The optimized conditions for performing the experiments were characterized for real-time detection of P. aeruginosa. The data acquisition system recorded the real-time light scattering from the test sample. After removing noise from the output waveform, five different time-domain statistical features were extracted from each waveform: standard mean, standard variance, skewness, kurtosis, and coefficient of variation. The pathogens classification was performed by training the discrimination model using extracted features based on machine learning algorithms. The support vector machines (SVM) with a sigmoid function kernel showed superior classification performance with 97.9% accuracy among other classifiers, including k-nearest neighbors (KNN), logistic regression (LR), and naïve Bayes (NB). The method can detect P. aeruginosa specifically and quantitatively with a limit of detection of 102 CFU/mL. The device can classify P. aeruginosa within 10 min with a total assay time of 25 min. The device was used to test its ability to detect pathogen from the serum and sputum specimens spiked with 105 CFU/mL concentration of P. aeruginosa. The results indicate that light scattering combined with machine learning can be used to detect P. aeruginosa. The proposed technique is anticipated to be helpful as a rapid device for diagnosing P. aeruginosa related infections.

2.
Front Nutr ; 8: 763677, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34722617

RESUMO

Background: Previous studies had revealed that Body Mass Index (BMI) positively affected Bone Mineral Density (BMD). However, an excessively high BMI was detrimental to health, especially for the elderly. Moreover, it was elusive how much BMI was most beneficial for BMD in older adults to maintain. Objective: To investigate whether there was a BMI saturation effect value that existed to maintain optimal BMD. Methods: A cross-sectional study was conducted using the datasets of the National Health and Nutrition Examination Survey (NHANES) 2005-2006, 2007-2008, 2009-2010, 2013-2014, and 2017-2018. After adjusting for covariates, an analysis of the association between BMI and BMD in different femoral regions (Total femur, Femoral neck, Trochanter, Intertrochanter, and Ward's triangle) and lumbar spine regions (Total spine, L1, L2, L3, and L4) in the whole population was performed using the multivariate linear regression models, smoothing curve fitting, and saturation effects analysis models. Then, subgroup analyses were performed according to gender, age, and race. Results: A total of 10,910 participants (5,654 males and 5,256 females) over 50 years were enrolled in this population-based study. Multivariate linear regression analyses in the population older than 50 years showed that BMI was positively associated with femoral BMD and lumbar spine BMD (P < 0.001, respectively). Smoothing curve fitting showed that the relationship between BMI and BMD was not simply linear and that a saturation value existed. The saturation effect analysis showed that the BMI saturation value was 26.13 (kg/m2) in the total femur, 26.82 (kg/m2) in the total spine, and showed site-specificity in L1 (31.90 kg/m2) and L2 (30.89 kg/m2). The saturation values were consistent with the whole participants in males, while there was high variability in the females. BMI saturation values remained present in subgroup analyses by age and race, showing specificity in some age (60-70 years old) groups and in some races. Conclusions: Our study showed a saturation value association between BMI and BMD for people over 50 years old. Keeping the BMI in the slightly overweight value (around 26 kg/m2) might reduce other adverse effects while obtaining optimal BMD.

3.
Sci Total Environ ; : 151189, 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34757105

RESUMO

Marine heatwaves (MHWs) are weather-timescale extreme events in the oceans and can have devastating effects on marine bivalves and ecosystems they support, with considerable socio-economic consequences. Yet, the extent to which marine bivalves have the capacity to acclimate and adapt to MHWs remains unknown. Understanding molecular responses to MHWs is imperative to develop strategies for conservation of ecologically and economically important marine organisms. Here, using RNA-Seq, we investigate how various MHWs scenarios elicit molecular changes in threatened and vulnerable pearl oysters, Pinctada maxima (Jameson). Acute exposure of MHWs - mimicked by rapid increases of seawater temperature from 24 °C to 28 °C and 32 °C, respectively - significantly affected the expression levels of metabolic and immune-related genes, with thermal stress-responsive genes especially like HSP20, HSP70 and HSP90 being remarkably up-regulated. Following repeat exposure to MHWs, encouragingly, pearl oysters exhibited evident acclimation responses, as best exemplified by significantly lowered expression levels of key stress-responsive genes involved in metabolism and immunity in comparison to those observed during acute exposure. Findings of the present study provide a better understanding of molecular processes underpinning the acclimation and adaptation of marine bivalves to MHWs in the context of climate change.

4.
ACS Sens ; 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34784175

RESUMO

Slow response and recovery kinetics is a major challenge for practical room-temperature NO2 gas sensing. Here, we report the use of visible light illumination to significantly shorten the response and recovery times of a PbSe quantum dot (QD) gel sensor by 21% (to 27 s) and 63% (to 102 s), respectively. When combined with its high response (0.04%/ppb) and ultralow limit of detection (3 ppb), the reduction in response and recovery time makes the PbSe QD gel sensor among the best p-type room-temperature NO2 sensors reported to date. A combined experimental and theoretical investigation reveals that the accelerated response and recovery time is primarily due to photoexcitation of NO2 gaseous molecules and adsorbed NO2 on the gel surface, rather than the excitation of the semiconductor sensing material, as suggested by the currently prevailing light-activated gas-sensing theory. Furthermore, we find that the extent of improvement attained in the recovery speed also depends on the distribution of excited electrons in the adsorbed NO2/QD gel system. This work suggests that the design of light-activated sensor platforms may benefit from a careful assessment of the photophysics of the analyte in the gas phase and when adsorbed onto the semiconductor surface.

5.
Int Urol Nephrol ; 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34807346

RESUMO

BACKGROUND: Researchers have proved that simple renal cysts (SRCs) might be correlated with renal dysfunction, but it is still controversial. Thus, we conducted clinical research study with large sample size and long-term follow-up to clarify the relationship between SRCs and renal dysfunction. METHODS: A total of 571 SRCs patients in outpatients of nephrology department were included, we investigated the clinical characteristics of growth SRCs compared with non-growth SRCs, evaluated the incidence of renal dysfunction in SRCs and explored the risk factors of renal dysfunction in growth SRCs. RESULTS: The mean baseline age was 51.31 ± 14.37 years in the whole cohort, ranging from 19 to 79 years, and 57.6% of them were male. The median follow-up duration was 3 years, ranging from 1 to 10 years. In addition, the final maximum diameter increased 1 mm (2.74%) per year. Patients in growth SRCs group tented to have higher percentage of hypertension, hematuria, large cyst and multiple cysts compared with non-growth SRCs group. The prevalence of renal dysfunction was 15.6% after the follow-up, and the prevalence of renal dysfunction was about 10 times higher in growth SRCs group than non-growth SRCs group (23.3% vs. 2.4%). Renal dysfunction was significantly associated with age, female, total cholesterol, diastolic blood pressure, final maximum diameter and yearly change in maximum diameter in growth SRCs. CONCLUSIONS: SRCs were closely related to the decline of renal function, we recommend close follow-up for growth SRCs.

6.
Front Genet ; 12: 720849, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34759953

RESUMO

Background: Chemotherapy resistance based on fluorouracil and cisplatin is one of the most encountered postoperative clinical problems in patients diagnosed with gastric cancer (GC), resulting in poor prognosis. Aim of the Study: This study aimed to combine autophagy-related genes (ARGs) to investigate the susceptibility patients with GC to postoperative chemotherapy. Methods: Based on The Cancer Genome Atlas (TCGA) database, gene expression data for GC patients undergoing chemotherapy were integrated and analyzed. Prognostic genes were screened based on univariate and multivariate analysis regression analysis. Subjects were divided into high-risk and low-risk groups according to the median risk score. Kaplan-Meier method was used to evaluate OS and DFS. The accuracy of the prediction was determined by the subject operating characteristic curve analysis. In addition, stratified analyses based on different clinical variables was performed to assess the correlation between risk scores and clinical variables. Quantitative real-time (qRT) PCR was used to verify the expression of CXCR4 in GC tissues and cell lines. Results: A total of nine ARGs related to the prognosis of chemotherapy patients were screened out. Compared with normal gastric mucosa cell, CXCR4 showed elevated expression in GC and was significantly associated with survival. Based on GEO and TCGA databases, the model accurately predicted DFS and OS after chemotherapy. Conclusion: This study established prognostic markers based on nine genes, predicting that ARGs are related to chemotherapy susceptibility of GC patients, which can provide better individualized treatment regimens for clinical practice.

7.
J Am Chem Soc ; 2021 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-34797057

RESUMO

Thermally activated delayed fluorescent (TADF) materials are promising to overcome triplet-induced optical loss in the pursuit of electrically pumped organic lasers. However, population inversion is difficult to establish in these materials due to the severe suppression of triplet-to-singlet upconversion in their condensed states. In this work, we report thermally activated lasing in solution-processed coassembled microcrystals, where TADF dyes were uniformly dispersed into crystalline matrices to ensure an efficient reverse intersystem crossing (RISC). The dark-state triplet excitons harvested by the RISC were effectively converted into radiative singlet excitons, which subsequently participated in the population inversion to boost lasing with an unusual temperature dependence. The lasing wavelength was tuned over the full visible spectrum by doping various TADF laser dyes, owing to the excellent compatibility. Trichromatic TADF microlasers were precisely patterned into periodic pixelated arrays by a template-confined solution-growth method. With as-prepared TADF microlaser arrays as display panels, vivid laser displays were achieved under programmable excitation. These results offer valuable enlightenment to minimize triplet state-related energy losses toward high-performance lasers.

8.
Zootaxa ; 5057(2): 181-200, 2021 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-34811214

RESUMO

Amolops splendissimus Orlov and Ho, 2007 and A. caelumnoctis Rao and Wilkinson, 2007 were described almost simultaneously from either side of the China-Vietnam border. The two species share a strong morphological resemblance, and their taxonomic distinctiveness has been questioned, yet no one has confirmed the taxonomic relationship and status between the two taxa. To resolve this taxonomic issue, we collected additional topotypic and near-topotypic specimens of A. splendissimus and A. caelumnoctis from both China (A. caelumnoctis: Wenshan County, Yunnan Province; type locality Luchun County, Yunnan Province), and Vietnam (A. splendissimus: Tam Duong District, Lai Chau Province; type locality Mount Ky Quan San, Bat Xat, Lao Cai Province). Molecular analysis based on a 16S rRNA fragment revealed minimal genetic divergences between the two taxa (0.0%0.4% uncorrected p-distance), and both species are closely related to A. viridimaculatus (2.1%2.3%) and A. medogensis (3.5%3.7%). Morphological comparisons between the newly collected specimens and the original descriptions of both species further support the lack of distinctiveness of the two species, hence, we conclude that A. caelumnoctis is a junior synonym of A. splendissimus.

9.
ACS Omega ; 6(43): 28804-28812, 2021 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-34746573

RESUMO

In this work, steel slag slurry was used in combination with O3 oxidation for the simultaneous removal of SO2 and NO x in a laboratory-scale wet flue gas desulfurization process. The effects of the oxidation temperature, steel slag concentration, initial SO2 concentration, and pH value on the desulfurization and denitrification efficiencies were studied. The results showed that the highest NO x removal efficiency occurred at an oxidation temperature of 90 °C. With an increase of the oxidation temperature above 90 °C, the denitrification efficiency decreased due to the decomposition of N2O5. The effect of the SO2 concentration on denitrification was complicated. When the concentration of SO2 was 500 ppm, generation of SO3 2- promoted the absorption of NO2. However, higher SO2 concentrations strengthened the competitive absorption of SO2 and NO x . In the pH range of 8.5-4.5, the denitrification efficiency was maintained at about 96%. The component analyses of the aqueous solution and the solid residue were conducted to investigate the compositions of the absorption products. The results showed that NO3 - and SO4 2- were the major anions in the aqueous solution. The nitrogen balance was analyzed to be 95.8%, clearly illustrating the migration and transformation path of nitrogen. In the solid residue, most alkaline substances were consumed, and the final products were mainly CaSO4 and FeO. Accordingly, the reaction mechanism of simultaneous desulfurization and denitrification using steel slag combined with ozone oxidation was proposed.

10.
BMC Cancer ; 21(1): 1117, 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34663242

RESUMO

BACKGROUND: Preoperative serum gamma-glutamyl transferase (γ-GT) levels is significantly related to the prognosis of hepatocellular carcinoma (HCC), but its clinical value in the management of postoperative adjuvant transarterial chemoembolization (PA-TACE) has rarely been explored. This study aimed to investigate whether γ-GT levels could be taken as a biomarker to guide the management of PA-TACE in resectable HCC. METHODS: HCC patients receiving radical resection were identified through the primary liver cancer big data (PLCBD) from December 2012 to December 2015. Prognostic factors of overall survival (OS) and disease-free survival (DFS) were identified by univariate and multivariate cox analyses, and subgroup analysis was conducted between PA-TACE group and non-TACE stratified by γ-GT levels before and after 1:1 propensity score matching (PSM). RESULTS: γ-GT level was found to be an independent risk factor of OS and DFS in 1847 HCC patients receiving radical resection (both P < 0.05), and patients with elevated γ-GT(> 54.0 U/L) have a shortened median OS and DFS, compared with those with normal γ-GT (both P < 0.001). In the subgroup of patients with normal γ-GT, there were no significant differences between groups of PA-TACE and non-TACE in terms of median OS and DFS before and after PSM (all P > 0.05), and PA-TACE was not a significant prognostic factor of both OS and DFS before and after PSM (all P > 0.05). In the subgroup of patients with elevated γ-GT, significant differences were found between groups of PA-TACE and non-TACE in terms of median OS and DFS before and after PSM (all P < 0.05), and PA-TACE was an independent prognostic factor of both OS and DFS (all P < 0.05). CONCLUSION: Currently, we concluded that patients with more advanced HCC also have more elevated γ-GT, and these patients with elevated γ-GT would be benefited more from PA-TACE after radical resection.

11.
Cell ; 184(22): 5559-5576.e19, 2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34678143

RESUMO

Glucose consumption is generally increased in tumor cells to support tumor growth. Interestingly, we report that glycogen accumulation is a key initiating oncogenic event during liver malignant transformation. We found that glucose-6-phosphatase (G6PC) catalyzing the last step of glycogenolysis is frequently downregulated to augment glucose storage in pre-malignant cells. Accumulated glycogen undergoes liquid-liquid phase separation, which results in the assembly of the Laforin-Mst1/2 complex and consequently sequesters Hippo kinases Mst1/2 in glycogen liquid droplets to relieve their inhibition on Yap. Moreover, G6PC or another glycogenolysis enzyme-liver glycogen phosphorylase (PYGL) deficiency in both human and mice results in glycogen storage disease along with liver enlargement and tumorigenesis in a Yap-dependent manner. Consistently, elimination of glycogen accumulation abrogates liver growth and cancer incidence, whereas increasing glycogen storage accelerates tumorigenesis. Thus, we concluded that cancer-initiating cells adapt a glycogen storing mode, which blocks Hippo signaling through glycogen phase separation to augment tumor incidence.

12.
Rev Environ Health ; 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34619029

RESUMO

Carbon nanomaterials (CNMs) are ubiquitous in our daily lives because of the outstanding physicochemical properties. CNMs play curial parts in industrial and medical fields, however, the risks of CNMs exposure to human health are still not fully understood. In view of, it is becoming extremely difficult to ignore the existence of the toxicity of CNMs. With the increasing exploitation of CNMs, it's necessary to evaluate the potential impact of these materials on human health. In recent years, more and more researches have shown that CNMs are contributed to the cancer formation and metastasis after long-term exposure through epithelial-mesenchymal transition (EMT) and cancer stem cells (CSCs) which is associated with cancer progression and invasion. This review discusses CNMs properties and applications in industrial and medical fields, adverse effects on human health, especially the induction of tumor initiation and metastasis through EMT and CSCs procedure.

13.
Artigo em Inglês | MEDLINE | ID: mdl-34705421

RESUMO

The low bioavailability of photosensitizers (PSs) and the hypoxia nature of tumors often limit the efficacy of current photodynamic therapy (PDT). Therefore, improving the utilization of three essential components (PS, light, and O2) in tumors will enhance PDT efficacy substantially. Herein, we have developed a red blood cell (RBC) biomimetic theranostic nanovesicle (named SPN-Hb@RBCM) with improved photostability, accumulation of PSs, and oxygen self-supply ability to enhance PDT efficacy upon near-infrared (NIR) laser irradiation. Such a biomimetic nanovesicle was prepared by a red blood cell membrane (RBCM)-camouflaged hemoglobin (Hb)-linked semiconducting polymer nanoparticle (SPN-Hb). The RBCM coating enables the long-term circulation of SPN-Hb due to the membrane-mediated immune evasion, allowing for more effective PS accumulation in tumors. Under 808 nm laser irradiation, the photostable SPN can serve as both a photodynamic and a second-near-infrared-window (NIR-II) fluorescence imaging agent; meanwhile, the conjugated Hb can be used as an oxygen carrier to relieve tumor hypoxia for enhancing PDT efficacy. In addition, Hb can also react with the tumor microenvironment overproduced H2O2 to generate cytotoxic hydroxyl radicals (•OHs) for chemodynamic therapy (CDT), which further achieve synergistic effects for PDT. Thus, this study proposed a promising biomimetic theranostic nanoagent for enhancing tumor oxygenation and NIR-II fluorescence-guided synergetic CDT/PDT against hypoxic tumors.

14.
J Phys Chem Lett ; 12(42): 10359-10365, 2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34668714

RESUMO

Two-dimensional (2D) graphitic carbon nitrides (g-C3N4) have sparked much interest as photocatalysts. However, they suffer from high activation energy and a low separation rate of photoexcited charge carriers. Here we report a viable strategy to craft one-dimensional carbon nitrides denoted as polymelem (PM). Our PM possesses NH-bridged and the N-bridged tautomers, both of which are π-conjugated polymers based on aromatic tri-s-triazine units, as revealed by solid-state nuclear magnetic resonance (NMR), Fourier transform infrared (FTIR), and Raman techniques. A 2D 1H double-quantum-single-quantum (DQ-SQ) NMR spectrum illustrates that the 2D architecture of PM is constructed by the formation of interchain N-H···N hydrogen bonds between different 1D PM chains. PM exhibits largely improved photocatalytic efficiency compared to g-C3N4. This can be attributed to the conjugated structures of PM, which are conducive to the decrease in activation energy and separation rate of photogenerated charge carriers.

15.
Aquat Toxicol ; 239: 105959, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34500377

RESUMO

In an era of unprecedented climate change, marine heatwaves (MHWs) are projected to increase in frequency, intensity, and duration, severely affecting marine organisms and fisheries and causing profound ecological and socioeconomic impacts. However, very little is known about effects of MHWs on ecologically and economically important bivalve species. Here, we investigate how pearl oysters, Pinctada maxima (Jameson), respond to MHWs under various simulated scenarios. Acute 3-day exposure to MHWs, mimicked by increasing the ambient seawater temperature from 24°C to 28°C, 32°C, and 36°C, respectively, induced significant changes (initially sustained increase and late decrease) in activities of antioxidant enzymes (GSH-Px, SOD, CAT, MDA, and T-AOC) and biomineralizaiton-related enzymes (AKP and ACP). Likewise, energy-metabolizing enzymes (NKA, CMA, and T-ATP) showed remarkable acute responses, with significant increases occurring at the beginning and end of thermal exposure. Following repeated exposure to MHWs, without exception, acute responses of assayed enzymes to MHWs were significantly alleviated, implying that pearl oysters have the ability to implement more efficient and less costly energy-utilizing strategies to compensate for thermal stress induced physiological interferences. These findings indicate that marine bivalves can respond plastically and acclimate rapidly to MHWs and pave the way for predicting the fate of this important taxonomic groups in rapidly changing oceans.


Assuntos
Pinctada , Poluentes Químicos da Água , Animais , Mudança Climática , Oceanos e Mares , Água do Mar , Poluentes Químicos da Água/toxicidade
16.
Front Mol Biosci ; 8: 721990, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34568427

RESUMO

Esophageal cancer (EC) is a common malignant disease in eastern countries. However, a study of the metabolomic characteristics associated with other biological factors in esophageal squamous cell carcinoma (ESCC) is limited. Interleukin enhancer binding factor 2 (ILF2) and ILF3, double-stranded RNA-binding proteins, have been reported to contribute to the occurrence and development of various types of malignancy. Nevertheless, the underlying functions of ILF2 and ILF3 in ESCC metabolic reprogramming have never been reported. This study aimed to contribute to the metabolic characterization of ESCC and to investigate the metabolomic alterations associated with ILF2 and ILF3 in ESCC tissues. Here, we identified 112 differential metabolites, which were mainly enriched in phosphatidylcholine biosynthesis, fatty acid metabolism, and amino acid metabolism pathways, based on liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry approaches using ESCC tissues and paired para-cancer tissues from twenty-eight ESCC patients. In addition, ILF2 and ILF3 expression were significantly elevated in EC tissues compared to the histologically normal samples, and closely associated with PI3K/AKT and MAPK signaling pathways in ESCC. Moreover, in ESCC tissues with a high ILF2 expression, several short-chain acyl-carnitines (C3:0, C4:0, and C5:0) related to the BCAA metabolic pathway and long-chain acyl-carnitines (C14:0, C16:0, C16:0-OH, and C18:0) involved in the oxidation of fatty acids were obviously upregulated. Additionally, a series of intermediate metabolites involved in the glycolysis pathway, including G6P/F6P, F1,6BP, DHAP, G3P, and 2,3BPG, were remarkably downregulated in highly ILF3-expressed ESCC tissues compared with the corresponding para-cancer tissues. Overall, these findings may provide evidence for the roles of ILF2 and ILF3 during the process of ESCC metabolic alterations, and new insights into the development of early diagnosis and treatment for ESCC. Further investigation is needed to clarify the underlying mechanism of ILF2 and ILF3 on acyl-carnitines and the glycolysis pathway, respectively.

17.
Chem Commun (Camb) ; 57(71): 8977-8980, 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34486619

RESUMO

This study reports a photoelectrochemical biosensor for dopamine-loaded liposome-encoded magnetic beads cleaved by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas 12a system for the quantification of human papilloma virus (HPV)-related DNA using neodymium-doped BiOBr nanosheets (Nd-BiOBr) as a photoactive matrix. Magnetic beads and dopamine-loaded liposomes are covalently attached to the both ends of ssDNA to construct dumbbell-shaped dopamine-loaded liposome-encoded magnetic bead (DLL-MB) probes. When the guide RNA binds to the target HPV-16, the ssDNA will be cleaved by Cas12a, thereby degrading the double dumbbell probes. After magnetic separation, the dissolved DLLs are treated with Triton X-100 to release the dopamine (as an electron donor), which was then detected by an amplified photocurrent using the Nd-BiOBr-based photoelectrode.


Assuntos
Proteínas de Bactérias/química , Técnicas Biossensoriais/métodos , Proteínas Associadas a CRISPR/química , Sistemas CRISPR-Cas , DNA Viral/análise , Endodesoxirribonucleases/química , DNA de Cadeia Simples/química , Dopamina/química , Técnicas Eletroquímicas/métodos , Fluoresceínas/química , Corantes Fluorescentes/química , Papillomavirus Humano 16/química , Lipossomos/química , Fenômenos Magnéticos , Processos Fotoquímicos
18.
Int J Med Robot ; : e2336, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34586687

RESUMO

BACKGROUND: Oesophagogastric anastomosis is mainly complicated by its tediousness. We hope to modify an oesophagogastric anastomotic technique that simplifies anastomosis. METHODS: We conducted a retrospective analysis of 57 cases executed using reverse-puncture anastomotic (RPA) technique and 64 cases of manual purse anastomosis (MPA) technique for robot-assisted minimally invasive oesophagectomy (RAMIE). Baseline characteristics and perioperative outcomes were analysed. RESULTS: There were no significant differences between the 2 groups with regards to demographic data and clinical features. All patients had R0 resection. Relative to MPA, RPA group experienced significantly shorter operation times (232.5 ± 33.84 min vs. 262.3 ± 83.94 min, p = 0.038).RPA group patients had shorter anastomotic times relative to MPA group patients (10.5 ± 3.4 min vs. 18.3 ± 4.1 min, p = 0.014). No adverse events were observed. CONCLUSIONS: Reverse-puncture anastomosis is safe, feasible in RAMIE. This approach has the potential to efficiently shorten the anastomotic time and ensure safe operation.

19.
ACS Appl Mater Interfaces ; 13(39): 46440-46450, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34547887

RESUMO

Pressure-based immunoassays have been studied for point-of-care testing for which increasing the sensitivity is still a challenge. In this study, we described an enhanced pressure-based immunoassay with a versatile electronic sensor for the sensitive biological analysis. The versatile electronic sensor had multifunctional sensing capabilities with temperature and pressure recording. Magnetic bead-modified capture antibody and platinum nanoparticle-labeled detection antibody were used as the biorecognition element of the target carcinoembryonic antigen (CEA) (as a model analyte) and would form a sandwich-type immune complex with CEA. After simple magnetic separation, this complex was transferred into the detection chamber, which contained both hydrogen peroxide (H2O2) and 3,3',5,5'-tetramethylbenzidine (TMB). With the catalytic ability of PtNPs, the "H2O2-TMB-PtNPs" system was catalyzed to generate a large amount of oxygen (O2) and photothermal agent of oxidizer TMB (ox-TMB). Meanwhile, in a sealed chamber, further irradiation with an 808 nm near-infrared laser led to a triple-step signal amplification strategy of pressure increase, temperature increase, and gas thermal expansion to receive a strong electrical signal through the electronic sensor in real time. Thus, the amplified electrical signal from the electronic sensor could reveal the target concentration. In addition, we also verified that the synergistic system with two physical quantities had a lower limit of detection and a wider detection range compared to the detection system with a single physical quantity. In general, this immunoassay not only helped in exploring an effective signal amplification pathway but also offered an opportunity for the development of versatile electronic sensors in point-of-care settings.

20.
Anal Chem ; 93(39): 13389-13397, 2021 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-34554727

RESUMO

Early diagnosis of cancers relies on the sensitive detection of specific biomarkers, but most of the current testing methods are inaccessible to home healthcare due to cumbersome steps, prolonged testing time, and utilization of toxic and hazardous substances. Herein, we developed a portable self-powered photoelectrochemical (PEC) sensing platform for rapid detection of prostate-specific antigen (PSA, as a model disease-related protein) by integrating a self-powered photoelectric signal output system catalyzed with chemiluminescence-functionalized Au nanoparticles (AuNPs) and a phosphomolybdic acid (PMA)-based photochromic visualization platform. TiO2-g-C3N4-PMA photosensitive materials were first synthesized and functionalized on a sensor chip. The sensor consisted of filter paper modified with a photocatalytic material and a regional laser-etched FTO electrode as an alternative to a conventional PEC sensor with a glass-based electrode. The targeting system involved a monoclonal anti-PSA capture antibody-functionalized Fe3O4 magnetic bead (mAb1-MB) and a polyclonal anti-PSA antibody (pAb2)-N-(4-aminobutyl)-N-ethylisoluminol-AuNP (ABEI-AuNP). Based on the signal intensity of the chemiluminescent system, the photochromic device color changed from light yellow to heteropoly blue through the PMA photoelectric materials integrated into the electrode for visualization of the signal output. In addition, the electrical signal in the PEC system was amplified by a sandwich-type capacitor and readout on a handheld digital multimeter. Under optimum conditions, the sensor exhibited high sensitivity relative to PSA in the range of 0.01-50 ng mL-1 with a low detection limit of 6.25 pg mL-1. The flow-through chemiluminescence reactor with a semiautomatic injection device and magnetic separation was avoid of unstable light source intensity inherent in the chemiluminescence process. Therefore, our strategy provides a new horizon for point-of-care analysis and rapid cost-effective clinical diagnosis.


Assuntos
Luminescência , Nanopartículas Metálicas , Ouro , Imunoensaio
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...