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1.
Korean J Radiol ; 21(4): 483-493, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32193896

RESUMO

OBJECTIVE: To evaluate the distribution and characteristics of peripheral nerve abnormalities in chronic inflammatory demyelinating polyneuropathy (CIDP) using magnetic resonance neurography (MRN) and to examine the diagnostic efficiency. MATERIALS AND METHODS: Thirty-one CIDP patients and 21 controls underwent MR scans. Three-dimensional sampling perfections with application-optimized contrasts using different flip-angle evolutions and T1-/T2- weighted turbo spin-echo sequences were performed for neurography of the brachial and lumbosacral (LS) plexus and cauda equina, respectively. Clinical data and scores of the inflammatory Rasch-built overall disability scale (I-RODS) in CIDP were obtained. RESULTS: The bilateral extracranial vagus (n = 11), trigeminal (n = 12), and intercostal nerves (n = 10) were hypertrophic. Plexus hypertrophies were observed in the brachial plexus of 19 patients (61.3%) and in the LS plexus of 25 patients (80.6%). Patterns of hypertrophy included uniform hypertrophy (17 [54.8%] brachial plexuses and 21 [67.7%] LS plexuses), and multifocal fusiform hypertrophy (2 [6.5%] brachial plexuses and 4 [12.9%] LS plexuses) was present. Enlarged and/or contrast-enhanced cauda equina was found in 3 (9.7%) and 13 (41.9%) patients, respectively. Diameters of the brachial and LS nerve roots were significantly larger in CIDP than in controls (p < 0.001). The largest AUC was obtained for the L5 nerve. There were no significant differences in the course duration, I-RODS score, or diameter between patients with and without hypertrophy. CONCLUSION: MRN is useful for the assessment of distribution and characteristics of the peripheral nerves in CIDP. Compared to other regions, LS plexus neurography is more sensitive for CIDP.

2.
J Am Heart Assoc ; 9(6): e014120, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32174233

RESUMO

Background Epidemiological studies have suggested an association between Helicobacter pylori (H pylori) infection and atherosclerosis through undefined mechanisms. Endothelial dysfunction is critical to the development of atherosclerosis and related cardiovascular diseases. The present study was designed to test the hypothesis that H pylori infection impaires endothelial function through exosome-mediated mechanisms. Methods and Results Young male and female patients (18-35 years old) with and without H pylori infection were recruited to minimize the chance of potential risk factors for endothelial dysfunction for the study. Endothelium-dependent flow-mediated vasodilatation of the brachial artery was evaluated in the patients and control subjects. Mouse infection models with CagA+ H pylori from a gastric ulcer patient were created to determine if H pylori infection-induced endothelial dysfunction could be reproduced in animal models. H pylori infection significantly decreased endothelium-dependent flow-mediated vasodilatation in young patients and significantly attenuated acetylcholine-induced endothelium-dependent aortic relaxation without change in nitroglycerin-induced endothelium-independent vascular relaxation in mice. H pylori eradication significantly improved endothelium-dependent vasodilation in both patients and mice with H pylori infection. Exosomes from conditioned media of human gastric epithelial cells cultured with CagA+ H pylori or serum exosomes from patients and mice with H pylori infection significantly decreased endothelial functions with decreased migration, tube formation, and proliferation in vitro. Inhibition of exosome secretion with GW4869 effectively preserved endothelial function in mice with H pylori infection. Conclusions H pylori infection impaired endothelial function in patients and mice through exosome-medicated mechanisms. The findings indicated that H pylori infection might be a novel risk factor for cardiovascular diseases.

3.
Opt Express ; 28(3): 2661-2682, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32121950

RESUMO

A methodology is developed for deriving consistent ocean biological and biogeochemical products from multiple satellite ocean color sensors that have slightly different sensor spectral characteristics. Specifically, the required coefficients for algorithm modifications are obtained using the hyperspectral in situ optical measurements from the Marine Optical Buoy (MOBY) in the water off Hawaii. It is demonstrated that using the proposed approach for modifying ocean biological and biogeochemical algorithms, satellite-derived ocean property data over the global open ocean are consistent from multiple satellite sensors, although their corresponding sensor-measured normalized water-leaving radiance spectra nLw(λ) are different. Therefore, the proposed approach allows satellite-derived ocean biological and biogeochemical products to be consistent and can therefore be routinely merged from various satellite ocean color sensors. The proposed approach can be applied to any satellite algorithms that use the input of sensor-measured nLw(λ) spectra.

4.
Hepatology ; 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-32154934

RESUMO

BACKGROUND & AIMS: Hepatocellular carcinoma (HCC) is associated with high malignancy rates. Recently, a known deacetylase SIRT1 is discovered in HCC, while its presence is positively correlated with malignancy and metastasis. N6 -methyladenosine (m6 A) is the most prominent modification but the exact mechanisms on how SIRT1 regulates m6 A modification to induce hepatocarcinogenesis remain unclear. APPROACH & RESULTS: Here we demonstrate that SIRT1 exerts oncogenic role by downregulating fat mass and obesity-associated protein (FTO), which is an m6 A demethylase. A crucial component of small ubiquitin-related modifiers (SUMOs) E3 ligase, RANBP2, is activated by SIRT1 and it is indispensable for FTO SUMOylation at Lysine (K)-216 site that promotes FTO degradation. Moreover, Guanine nucleotide-binding protein G (o) subunit alpha (GNAO1) is firstly identified as m6 A downstream targets of FTO and tumor suppressor in HCC, and depletion of FTO by SIRT1 improves m6 A+ GNAO1 and downregulates its mRNA expression. CONCLUSIONS: We demonstrate an important mechanism whereby SIRT1 destabilizes FTO, steering the m6 A+ of downstream molecules and subsequent mRNA expression in HCC tumorigenesis. Our findings uncover a novel target of SIRT1 for therapeutic agents to treat HCC.

5.
Cell Cycle ; 19(6): 711-725, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32089063

RESUMO

A long noncoding RNA called SNHG17 (small nucleolar RNA host gene 17) is aberrantly expressed and plays essential roles in multiple human cancer types. Nevertheless, its expression pattern and specific functions in tongue squamous cell carcinoma (TSCC) have not been well studied until now. Hence, in this study, we aimed to measure SNHG17 expression in TSCC and to examine the actions of SNHG17 on the malignant characteristics of TSCC cells. The regulatory mechanism that mediates the oncogenic effects of SNHG17 on TSCC cells was investigated too. In this study, SNHG17 was found to be upregulated in TSCC, and this overexpression closely correlated with adverse clinical parameters and shorter overall survival among the patients with TSCC. The SNHG17 knockdown significantly decreased TSCC cell proliferation, migration, and invasion in vitro and tumor growth in vivo. Mechanism investigation revealed that SNHG17 acts as a competing endogenous RNA on microRNA-876 (miR-876) in TSCC cells. In addition, specificity protein 1 (SP1) was validated as a direct target gene of miR-876 in TSCC cells. SP1 expression restoration in TSCC cells reversed miR-876 overexpression-induced anticancer effects. MiR-876 downregulation strongly attenuated the actions of the SNHG17 knockdown in TSCC cells. SNHG17 plays an oncogenic part in TSCC cells both in vitro and in vivo via sponging of miR-876 and thereby upregulating SP1, which could be regarded as a promising target for TSCC therapy.

6.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(1): 225-229, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32027281

RESUMO

OBJECTIVE: To analyze the clinical efficacy and side effects of reduced-dose of cyclophosphamide combined cyclosporine A for severe aplastic anemia(SAA) children. METHODS: Ten pediatric patients with SAA from January 2008 to May 2012 were enrolled. All the patients were treated with reduced dose of cyclophosphamide combined cyclosporine A. The dose of cyclophosphamide was 30 mg/(kg·d)×4 d, the dose of cyclosporine A gradually increased >15 mg/L accroding to the blood concentration. RESULTS: The median follow-up time of the 10 pediatric patients was 100 months (6-126 months). Among 10 children with SAA, 4 cases achieved complete response(CR), 3 cases obtained partial response (PR) and the overall response rate was 70%, the remaining 3 cases showed no response (NR). One refractory patient treated by cyclophosphamide was progressed to paroxysmal nocturnal hemoglobinuria(PNH) at 25 months and was dead at 42 months after therapy. CONCLUSION: The results show that reduced-dose cyclophosphamide (30 mg/kg·d for 4 consecutive days) combinated with CsA (initial dose 4 mg/kg·d, and drugvallery concentration >150 ng/ml) can make 7 of 10 children with severe aplastic anemia achieve complete response or partial response, and this regimen may be the second line regimen selected for some SAA children.


Assuntos
Anemia Aplástica , Soro Antilinfocitário , Criança , Ciclofosfamida , Ciclosporina , Hemoglobinúria Paroxística , Humanos , Imunossupressores , Resultado do Tratamento
7.
J Hazard Mater ; 390: 122188, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-32006843

RESUMO

Electrolytic manganese residue (EMR) is a potentially harmful industrial solid waste that should be addressed. In the study, the red mud, carbide slag and blast furnace slag were used as stabilization/solidification (S/S) agents to S/S Mn2+, and simultaneous reused it as raw material to prepare road base material. The S/S behavior of manganese, unconfined compressive strength (UCS) of road base material with different Al/Si ratios, leaching test and the S/S mechanisms were investigated. The results showed that the Mn2+ can be well solidified when the S/S agents reach up to 20 %. The 7-day UCS of the road base material was 6.1 MPa with the Al/Si ratio of 0.48, which meets the highway standards. When Al/Si = 0.48, the formation amount of CaAl2Si2O8·4H2O and ettringite increased, which promoted the adsorption and wrap of Mn2+. The content of active AlⅣ and AlⅥ increased after S/S. Mn2SiO4 and Ca4Mn4Si8O24 were produced by the charge balance effect, and the new chemical bond was formed. Meanwhile, the Mn2+ is oxidized to more stable MnO2 to achieve the S/S of Mn2+. This research provides an effective way to solidify Mn2+ and solves the problem of large-scale utilization of EMR and other solid waste.

8.
Curr Gene Ther ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32072883

RESUMO

OBJECTIVE: In order to generate induced pluripotent stem cells (iPSCs) more efficiently, it is crucial to identify somatic cells that are easily accessible and possibly require fewer factors for conversion into iPSCs. METHOD: Human epidermal melanocytes were transduced with lentiviral vectors carrying 3transcription factors (OCT-4, KLF-4 and c-MYC, 3F) or 4 transcriptionfactors (OCT-4, KLF-4, c-MYC and SOX-2, 4F). Once the clones had formed, assays related to stem cell pluripotency, including alkaline phosphatase staining, DNA methylation levels, expression of stem cell markers and ultrastructure analysis were carried out. The iPSCs obtained were then induced to differentiate into the cells representing the three embryonic layers in vitro. RESULTS: Seven days after transduction of epidermal melanocytes with 3F or 4F, clones were formed that were positive for alkaline phosphatase staining. Fluorescent staining with antibodies against OCT-4andSOX-2was strongly positive, and the cells showed a high nucleus-cytoplasmratio and active karyokinesis. No melanosomes were found in the cytoplasm by ultrastructural analysis. There were obvious differences in DNA methylation levels between the cloned cells and their parental cells. However, there was not a significant difference between 3F or 4F transfected clonal cells. Meanwhile, the iPSCs successfully differentiated into the three germ layer cells in vitro. CONCLUSION: Human epidermal melanocytes do not require ectopic SOX-2 expression for conversion into iPSCs, and may serve as an alternative source for deriving patient-specific iPSCs with fewer genetic elements.

9.
BMC Musculoskelet Disord ; 21(1): 77, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32024487

RESUMO

BACKGROUND: The leptin receptor-deficient knockout (db/db) mouse is a well-established model for studying type II diabetes mellitus (T2DM). T2DM is an important risk factor of intervertebral disc degeneration (IVDD). Although the relationship between type I diabetes and IVDD has been reported by many studies, few studies have reported the effects of T2DM on IVDD in db/db mice model. METHODS: Mice were separated into 3 groups: wild-type (WT), db/db, and IGF-1 groups (leptin receptor-deficient mice were treated with insulin-like growth factor-1 (IGF-1). To observe the effects of T2DM and glucose-lowering treatment on IVDD, IGF-1 injection was used. The IVD phenotype was detected by H&E and safranin O fast green staining among db/db, WT and IGF-1 mice. The levels of blood glucose and weight in mice were also recorded. The changes in the mass of the trabecular bone in the fifth lumbar vertebra were documented by micro-computed tomography (micro-CT). Tunnel assays were used to detect cell apoptosis in each group. RESULTS: The weight of the mice were 27.68 ± 1.6 g in WT group, which was less than 57.56 ± 4.8 g in db/db group, and 52.17 ± 3.7 g in IGF-1 injected group (P < 0.05). The blood glucose levels were also significantly higher in the db/db mice group. T2DM caused by leptin receptor knockout showed an association with significantly decreased vertebral bone mass and increased IVDD when compared to WT mice. The db/db mice induced by leptin deletion showed a higher percentage of MMP3 expression as well as cell apoptosis in IVDD mice than WT mice (P < 0.05), while IGF-1 treatment reversed this situation (P < 0.05). CONCLUSIONS: T2DM induced by leptin receptor knockout led to IVDD by increasing the levels of MMP3 and promoting cell apoptosis. IGF-1 treatment partially rescue the phenotype of IVDD induced by leptin receptor knockout.

10.
Waste Manag ; 103: 342-351, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31923841

RESUMO

Application of thermophilic composting (TC) is limited due to poor efficiency and long composting period. Hyperthermophilic composting (HTC) could effectively overcome this defect. Here, the transformation of humic acid (HA) in both HTC and TC was characterized and compared to investigate the roles of HTC toward accelerating the formation of HA. In HTC, the highest temperature was 96.6 °C, and the hyperthermophilic and thermophilic phases exceed 18 days. The degree of polymerization (DP) in HTC increased to 1.27 on day 27, while it only increased to 1.15 at the end of TC. The elemental composition of the HA in HTC showed higher O atomic content (36.3%) and lower C/N atomic ratio (6.5) compared with TC. These changes indicated that HTC could significantly accelerate oxidized and polycondensed reactions for HA formation, which resulted in the shortening of composting period to 27 days. The maximum fluorescence intensity (Fmax) of humic-like components were achieved faster in HTC (Fmax = 1649.9) than in TC (Fmax = 1316.9), implying that HTC promoted the polycondensation of small molecular components to form HA with larger molecular weight and higher degree of aromatization. Two-dimensional FTIR correlation spectroscopy (2D-FTIR-COS) analysis demonstrated that HTC prevented the HA precursor from condensing before it was deeply oxidized, and increased the content of small molecules rich in carboxyl moieties. Based on the evolution of the molecular structure of HA, the level of oxidation of HA precursors was a key factor to determine the degree of polymerization and the degree of HA humification.

11.
Rapid Commun Mass Spectrom ; 34(8): e8690, 2020 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-31908079

RESUMO

RATIONALE: An inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) instrument can be developed to determine 87 Sr/86 Sr ratios with an external precision better than 0.05% relative standard deviation (RSD) in "mass shift" mode without prior Sr purification. Previous studies suggested using CH3 F, N2 O, and SF6 as reaction gases for this method because a better reaction rate can be achieved with Sr+ than with O2 in the reaction cell. However, these gases are not commonly used in general chemistry laboratories, and processes using these gases are difficult to implement quickly due to regulations. We aim to develop a rapid method that can be applied to many samples for the accurate determination of 87 Sr/86 Sr isotope ratios with precision below 0.1% RSD (or approximately to the fourth decimal place). METHODS: We evaluated the accuracy and precision of 87 Sr/86 Sr ratios in certified reference materials and different rock types determined using ICP-MS/MS with O2 as the reaction gas in comparison with those determined using the multicollector inductively coupled plasma mass spectrometry (MC-ICP-MS) method. RESULTS: This study showed that by using the ICP-MS/MS method, the 87 Sr/86 Sr ratios of BCR-2 and BHVO-2 do not vary significantly with and without prior Sr purification; when the Sr concentration of the measured solution is within the range of 60-350 ng/mL, there is no significant effect on the measured 87 Sr/86 Sr ratios. The results also showed that the 87 Sr/86 Sr ratios of 23 different rock types measured by ICP-MS/MS and MC-ICP-MS methods agree very well. CONCLUSIONS: The precision of the 87 Sr/86 Sr ratio measured using ICP-MS/MS varies between 0.0001 and 0.0019 (2SD). This precision is less than that of the MC-ICP-MS method but is sufficient for certain applications, such as identifying 87 Sr/86 Sr ratios in different rock types. These results suggest that the developed ICP-MS/MS method has the potential for future studies involving the identification of Sr sources.

12.
IEEE Trans Pattern Anal Mach Intell ; 42(3): 664-678, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30530314

RESUMO

Face alignment and 3D face reconstruction are traditionally accomplished as separated tasks. By exploring the strong correlation between 2D landmarks and 3D shapes, in contrast, we propose a joint face alignment and 3D face reconstruction method to simultaneously solve these two problems for 2D face images of arbitrary poses and expressions. This method, based on a summation model of 3D faces and cascaded regression in 2D and 3D shape spaces, iteratively and alternately applies two cascaded regressors, one for updating 2D landmarks and the other for 3D shape. The 3D shape and the landmarks are correlated via a 3D-to-2D mapping matrix, which is updated in each iteration to refine the location and visibility of 2D landmarks. Unlike existing methods, the proposed method can fully automatically generate both pose-and-expression-normalized (PEN) and expressive 3D faces and localize both visible and invisible 2D landmarks. Based on the PEN 3D faces, we devise a method to enhance face recognition accuracy across poses and expressions. Both linear and nonlinear implementations of the proposed method are presented and evaluated in this paper. Extensive experiments show that the proposed method can achieve the state-of-the-art accuracy in both face alignment and 3D face reconstruction, and benefit face recognition owing to its reconstructed PEN 3D face.

13.
Nanoscale ; 12(3): 1384-1388, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31872848

RESUMO

One Yb42 nanowheel [Yb42L14(OH)28(OAc)84] was constructed using a tridentate vanillin ligand. The external diameter of the wheel-like structure is about 3.6 nm, which allows direct visualization by TEM. It shows interesting NIR lanthanide luminescence sensing towards anions, especially to fluoride at the ppm level.

14.
J Dairy Sci ; 103(1): 166-171, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31704010

RESUMO

The ability to use lactose is critical for the application of Streptococcus thermophilus in fermented dairy products. Most studies have evaluated the use of lactose of S. thermophilus by measuring lactose utilization, but its correlation with ß-galactosidase and urease has rarely been investigated. In this study, 10 strains of S. thermophilus isolated from fermented yak milk exhibited a diversity of ß-galactosidase and urease activities, growth, and acid production in de Man, Rogosa, and Sharpe-lactose. Among the strains, 15G5 possessed the highest ß-galactosidase activity and showed the highest cell growth, lactic acid production, and titratable acidity during fermentation. In contrast, 7G10, with the weakest ß-galactosidase activity, produced the lowest lactic acid content and change in titratable acidity. Further investigation indicated that ß-galactosidase activity of S. thermophilus showed significant positive correlations with the growth of cell densities, the production of lactic acid, and titratable acidity, and urease activity of S. thermophilus showed a significant correlation with the use of lactose and the production of lactic acid and acetaldehyde. These findings suggest that the differences of ß-galactosidase and urease activities are essential for the performance in the lactose metabolism, growth, and acid production of S. thermophilus, providing new insights into strain selection and application.

15.
J Dairy Sci ; 103(1): 87-97, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31677844

RESUMO

Previous research reported that fermented yak milk had a diverse microbial composition. For this study, raw yak milk, qula, and fermented yak milk samples were collected from the Aba Tibetan autonomous region of China. The genus and species microbial composition of these samples were analyzed by high-throughput sequencing of 16S rRNA and groEL gene amplicons, and the volatile profile of the samples was quantified by gas chromatography-mass spectrometry. The results indicated variation in abundance of microbiota at the genus level among the fermented yak milk samples, with Lactobacillus as the most abundant genus in the majority of samples, ranging from 41.6 to 98.3%. The volatile profile of the samples varied among those collected from different villages. Correlations between bacterial composition and volatile compounds of the samples were also observed. Lactobacillus displayed a significant correlation with volatile compounds such as benzaldehyde, 2,3-pentanedione, ethanol, and ethyl acetate, whereas the samples with relatively high abundance of Streptococcus and Lactococcus displayed relatively low contents of volatile compounds.


Assuntos
Bovinos , Produtos Fermentados do Leite/microbiologia , Lactobacillus/isolamento & purificação , Lactococcus/isolamento & purificação , Streptococcus/isolamento & purificação , Animais , Reatores Biológicos , China , Fermentação , Lactobacillus/genética , RNA Ribossômico 16S/genética
16.
J Hazard Mater ; 381: 120936, 2020 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-31377577

RESUMO

As a by-product of nickel production, the ferronickel slag (FNS) puts a lot of pressure on the environment. It is becoming more and more urgent to deal with the increasing FNS. The aim of this study is to explore the durability of concrete with FNS fine aggregate. Two kinds of FNS with different storage time were selected. The radioactivity detection, XRD test and stability detection of FNS were conducted to ensure FNS can be used as construction materials. Then the durability of concrete with 13%, 27%, 40% and 50% FNS (by weight of fine aggregate) was investigated, respectively. It was found that the properties of concrete prepared from FNS with different storage time had little difference. The results indicated that 27% FNS replacement showed improvement in resistance to sulfate attack by 22% but the resistance to chloride ion penetration was not significantly influenced. Moreover, 40% FNS addition brought a 33% abrasion reduction than that of original concrete. SEM analysis showed that FNS produced more C-S-H gels and improved the microstructure of concrete. This study indicated that proper content of FNS can be used as fine aggregate and it was beneficial to the durability of concrete, especially to the abrasion resistance.

17.
F1000Res ; 8: 1135, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31824661

RESUMO

Background: Basic and clinical scientific research at the University of South Florida (USF) have intersected to support a multi-faceted approach around a common focus on rare iron-related diseases. We proposed a modified version of the National Center for Biotechnology Information's (NCBI) Hackathon-model to take full advantage of local expertise in building "Iron Hack", a rare disease-focused hackathon. As the collaborative, problem-solving nature of hackathons tends to attract participants of highly-diverse backgrounds, organizers facilitated a symposium on rare iron-related diseases, specifically porphyrias and Friedreich's ataxia, pitched at general audiences. Methods: The hackathon was structured to begin each day with presentations by expert clinicians, genetic counselors, researchers focused on molecular and cellular biology, public health/global health, genetics/genomics, computational biology, bioinformatics, biomolecular science, bioengineering, and computer science, as well as guest speakers from the American Porphyria Foundation (APF) and Friedreich's Ataxia Research Alliance (FARA) to inform participants as to the human impact of these diseases. Results: As a result of this hackathon, we developed resources that are relevant not only to these specific disease-models, but also to other rare diseases and general bioinformatics problems. Within two and a half days, "Iron Hack" participants successfully built collaborative projects to visualize data, build databases, improve rare disease diagnosis, and study rare-disease inheritance. Conclusions: The purpose of this manuscript is to demonstrate the utility of a hackathon model to generate prototypes of generalizable tools for a given disease and train clinicians and data scientists to interact more effectively.

18.
Sci Rep ; 9(1): 18562, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31811220

RESUMO

This work presents the measurement of the permittivities of water and methanol in the D-band. Water is a reference medium for dielectric measurement. The dielectric permittivity of water in the millimeter wave range is a fundamental parameter in many applications, and needs to be investigated systematically. The measurement is conducted using a quasi-optical spectroscopy, which is an improved free-space method more suitable for the millimeter wave range. The theoretical formulae are derived using the signal-flow chart method, which is developed specially for multi-layer operation. This model enables one measure liquid samples. A non-calibration method has been developed to retrieve the permittivity. Water and methanol are measured at several temperatures. The measured results agree with published results in a 4% discrepancy. This work will add new measured data to the permittivities of water and methanol over the whole D-band.

19.
J Invest Surg ; : 1-8, 2019 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-31851859

RESUMO

Purpose: We aimed to investigate the role of microRNA-5100 (miRNA-5100) in oral squamous cell carcinoma (OSCC) and its underlying mechanisms.Material/Methods: The expression of miR-5100 and suppressor of cancer cell invasion (SCAI) in OSCC cell lines were examined. A luciferase reporter assay was applied to confirm the combination between miR-5100 and SCAI. Then, miR-5100 inhibitor or small hairpin RNA (shRNA)-SCAI were transfected into cells. Cell Counting Kit-8 assay was executed for testing cell proliferation ability. Flow cytometry assay was exploited for measuring cell cycle. Invasion and migration of OSCC cells were assessed using Transwell assay and wound healing assay. The expression of proteins were detected using western blotting.Results: The results demonstrated that the level of miR-5100 was upregulated while SCAI was downregulated in OSCC cells. SCAI was verified as a direct target of miR-5100. MiR-5100 silencing suppressed proliferation of OSCC cells, increased cells in the G1 and G2 phases, and reduced those in the S phase, which was reversed after transfection with shRNA-SCAI. Moreover, miR-5100 inhibitor downregulated the expression of cyclin-dependent kinase-2 (CDK-2) and cyclinD1, accompanied by upregulation in p27 expression, whereas SCAI silencing had the opposite results. The invasion and migration abilities of OSCC cells were reduced after treatment with miR-5100 inhibitor, whereas SCAI silencing suppressed the effects of miR-5100 inhibitor on OSCC cell behaviors.Conclusion: These findings suggested that miR-5100 silencing inhibit proliferation, invasion and migration of OSCC cells via upregulating the expression of SCAI, which provides theoretical basis and treatment strategies for the treatment of OSCC.

20.
Arch Dermatol Res ; 2019 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-31873772

RESUMO

Etanercept biosimilar recombinant human tumour necrosis factor-α receptor II: IgG Fc fusion protein (rhTNFR-Fc, trade name Yisaipu) has shown good efficacy in the treatment of moderate-to-severe plaque psoriasis. To compare the efficacy and safety of rhTNFR-Fc plus methotrexate (MTX) and rhTNFR-Fc plus placebo in Chinese patients with moderate-to-severe plaque psoriasis. In this multicentre, randomized, placebo-controlled trial, patients with moderate-to-severe plaque psoriasis were enrolled and randomly assigned in a 1:1 ratio to receive rhTNFR-Fc plus MTX or rhTNFR-Fc plus placebo. The primary endpoint was the proportion of patients achieving Psoriasis Area and Severity Index improvement of at least 75% (PASI 75) from baseline at week 24. Adverse events (AEs) were recorded to evaluate safety. Efficacy analysis was performed using the intent-to-treat principle. A total of 466 patients were enrolled and randomly received rhTNFR-Fc plus MTX (combination group, n = 233) or rhTNFR-Fc plus placebo (monotherapy group, n = 233). PASI 75 at week 24 was significantly higher in the combination group than in the monotherapy group (81.86% vs. 65.50%, p < 0.001). Similar results were observed in other PASI improvement scores at week 12 [PASI 75, 62.39% vs. 44.54% (p < 0.001); PASI 50, 87.17% vs. 75.55% (p = 0.001); and PASI 90, 34.07% vs. 18.78% (p < 0.001)] and week 24 [PASI 50, 92.48% vs. 85.59% (p = 0.019); and PASI 90, 64.16% vs. 42.36% (p < 0.001)]. Significantly more patients had a static Physicians' Global Assessment of clear or almost clear in the combination group than in the monotherapy group at week 12 (26.46% vs. 12.50%, p < 0.001) and week 24 (62.38% vs. 40.83%, p < 0.001). The most common AEs in the two groups were upper respiratory tract infection and abnormal liver function. The combination therapy of rhTNFR-Fc plus MTX was an effective therapy for moderate-to-severe plaque psoriasis with an acceptable safety and tolerability profile, indicating that it was feasible and well tolerated for patients.

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