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1.
Int J Mol Med ; 51(3)2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36704848

RESUMO

Subsequently to the publication of the above article, and a Corrigendum that was published with the intention of rectifying the issue of overlapping data panels showing cell migration and invasion assay data in Fig. 8 (DOI: 10.3892/mmr.2018.9415; published online on September 24, 2017), it was drawn to the Editors' attention by a concerned reader that certain of the data shown for the epithelial­mesenchymal transition experiments in Fig. 2B, western blotting data in Fig. 6 and scratch­wound assay data shown in Fig. 7A were strikingly similar to data appearing in different form in other articles by different authors at different research institutes, which had already been published elsewhere prior to this paper's submission to International Journal of Molecular Medicine. In addition, several other instances of overlapping data panels were identified in Fig. 8. Owing to the fact that a substantial number of contentious data included in this paper had already been published elsewhere prior to its submission to International Journal of Molecular Medicine, the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive a reply. The Editor apologizes to the readership for any inconvenience caused. [International Journal of Molecular Medicine 40: 1114­1124, 2017; DOI: 10.3892/ijmm.2017.3118].

2.
Thorac Cancer ; 2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36594104

RESUMO

BACKGROUND: Immune checkpoint inhibitors (ICIs) have become one important therapeutic strategy for advanced non-small-cell lung cancer (NSCLC). It remains imperative to identify reliable and convenient biomarkers to predict both the efficacy and toxicity of immunotherapy, and tumor-associated autoantibodies (TAAbs) are recognized as one of the promising candidates for this. PATIENTS AND METHODS: This study enrolled 97 advanced NSCLC patients with ICI-based immunotherapy treatment, who were divided into a training cohort (n = 48) and a validation cohort (n = 49), and measured for the serum level of 35 TAAbs. According to the statistical association between the serum positivity and clinical outcome of each TAAb in the training cohort, a TAAb panel was developed to predict the progression-free survival (PFS), and further examined in the validation cohort and in different subgroups. Similarly, another TAAb panel was derived to predict the occurrence of immune-related adverse events (irAEs). RESULTS: In the training cohort, a 7-TAAb panel composed of p53, CAGE, MAGEA4, GAGE7, UTP14A, IMP2, and PSMC1 TAAbs was derived to predict PFS (median PFS [mPFS] 9.9 vs. 4.3 months, p = 0.043). The statistical association between the panel positivity and longer PFS was confirmed in the validation cohort (mPFS 11.1 vs. 4.8 months, p = 0.015) and in different subgroups of patients. Moreover, another 4-TAAb panel of BRCA2, MAGEA4, ZNF768, and PARP TAAbs was developed to predict the occurrence of irAEs, showing higher risk in panel-positive patients (71.43% vs. 28.91%, p = 0.0046). CONCLUSIONS: Collectively, our study developed and validated two TAAb panels as valuable prognostic biomarkers for immunotherapy.

3.
Oncol Rep ; 49(2)2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36633144

RESUMO

4­Methoxydalbergione (4­MD) can inhibit the progression of certain types of cancer; however, its effects on esophageal cancer (EC) remain unclear. The present study aimed to investigate the inhibitory effect of 4­MD on EC and its molecular mechanism. ECA­109 and KYSE­105 cells were treated with or without lipopolysaccharide (LPS) and 4­MD. Cell Counting Kit­8 and colony formation assays were used to analyze cell proliferation. Wound healing assay was performed to evaluate cell migration. ELISA and western blotting were performed to measure the expression levels of NF­κB and inflammatory cytokines. In cells treated with 4­MD, proliferation and migration were significantly inhibited, the levels of inflammatory cytokines were downregulated and the NF­κB signaling pathway was inactivated. Notably, proliferation, migration, inflammation and NF­κB were promoted by LPS, whereas 4­MD reversed the increases induced by LPS in EC cells. In conclusion, 4­MD may attenuate the proliferation and migration of EC cells by inactivating the NF­κB signaling pathway.


Assuntos
Neoplasias Esofágicas , NF-kappa B , Humanos , NF-kappa B/metabolismo , Lipopolissacarídeos/farmacologia , Linhagem Celular Tumoral , Neoplasias Esofágicas/genética , Proliferação de Células , Citocinas/metabolismo , Movimento Celular , Regulação Neoplásica da Expressão Gênica
4.
World J Gastrointest Oncol ; 15(1): 69-75, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36684044

RESUMO

Gastric cancer (GC) is a malignant tumor originating from the gastric epithelium, and its incidence and mortality rates rank third among all malignant tumors worldwide. It is also one of the most common cancers in China and is treated predominantly by Western medicine in clinical practice. However, with the advancements in medical technology and informatics, the values of traditional Chinese medicine (TCM) in preventing and treating GC and improving prognosis have increasingly been recognized. According to TCM, clinical manifestations of GC can be divided into Yege (dysphagia), regurgitation, stomach pain, and Zhengxia (abdominal mass). Due to the unbalanced distribution of health care resources in China, most GC patients already have progressive or advanced-stage disease at the first diagnosis. As a result, most GC patients have poor physical function, and surgery or chemotherapy alone will aggravate the impairment to the immune function and seriously affect the quality of life. In contrast, TCM therapies have shown promising efficacy in the management of these patients. Here we review the role of the integrated TCM and Western medicine in treating advanced GC.

5.
Pain Res Manag ; 2023: 7088004, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36686371

RESUMO

Objective: The purpose of the present study (a randomized clinical trial) was to evaluate the preemptive analgesic effects of pregabalin combined with celecoxib in total knee arthroplasty (TKA). Methods: From January 2019 to June 2021, we enrolled 149 patients who underwent TKA and divided them into four groups: the placebo group (n = 36), celecoxib group (n = 38), pregabalin group (n = 38), and combination group (n = 37). Each group was given the corresponding preemptive analgesia regimen at 12 and 2 hours before surgery. The pain score at rest and upon movement, cumulative dosage of sufentanil, knee range of motion (ROM), high-sensitivityC-reactive protein (hs-CRP) level, and adverse effects were evaluated after TKA to compare the effects of the preemptive analgesia regimens among the four groups. Results: The pain scores upon movement were significantly lower in the combination group than in the other three groups at 6, 12, 24, and 48 hours after surgery (P < 0.05). The cumulative dose of sufentanil within 48 hours after surgery was lowest in the combined group among the four groups (P < 0.05). Hs-CRP, ROM, and postoperative nausea and vomiting (PONV) were within 72 hours after surgery significantly improved in the combination group compared with those of the three other groups (P < 0.05). Conclusion: The preemptive analgesia regimen of pregabalin combined with celecoxib had positive effects on improving acute pain and reducing the cumulative dose of opioids after TKA. This trial is registered with ChiCTR2100041595.


Assuntos
Analgesia , Artroplastia do Joelho , Humanos , Celecoxib/uso terapêutico , Pregabalina/uso terapêutico , Artroplastia do Joelho/efeitos adversos , Sufentanil , Estudos Prospectivos , Proteína C-Reativa , Analgésicos Opioides/uso terapêutico , Dor/etiologia , Dor Pós-Operatória/tratamento farmacológico , Dor Pós-Operatória/etiologia , Método Duplo-Cego
6.
J Environ Manage ; 330: 117138, 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36623387

RESUMO

Fe2O3-assisted pyrolysis has been demonstrated to be a cost-effective thermal desorption (TD) technology. Lurgi-Thyssen dust (LTD) is a type of steel slag waste that contains a large amount of Fe2O3. In this study, to reduce energy consumption, LTD was added to contaminated soil to evaluate the feasibility of enhancing the TD removal efficiency of di-(2-ethylhexyl) phthalate (DEHP). The DEHP removal rate increased by 22.39% after adding 2% LTD at 200 °C for 20 min. Because of the catalytic pyrolysis of LTD, DEHP was pyrolyzed to form three types of short-chain esters: mono-(2-ethylhexyl) phthalate (MEHP), di (2-methylbutyl) ester, and methyl 2-ethylhexyl phthalate. The pyrolysis products of DEHP were less toxic and did not affect soil reuse. When the DEHP removal rate was 87.10%, LTD addition decreased the temperature and residence time of TD and alleviated the effect of TD on the soil physicochemical properties. Additionally, the desorption of DEHP from soil fitted the pseudo-second-order kinetic model well. Thus, the addition of LTD to contaminated soil enhanced the efficiency of TD remediation. Moreover, this study could provide a practical and economical strategy for LTD reuse.

7.
Artigo em Inglês | MEDLINE | ID: mdl-36695669

RESUMO

Background: Acinetobacter baumannii-mediated bacterial pneumonia is a common disease that is harmful to human health. Dipalmitoylphosphatidylcholine (DPPC) is the major lipid component of the pulmonary surfactant (PS) found in the alveolar space; the PS helps to keep surface tension low, which allows for improved oxygen delivery. Resveratrol (RE) is a phytoalexin found in plants that is released in response to injury or infection. The therapeutic effect of Re is limited due to its low solubility and bioavailability. In this study, we report pulmonary delivery of Re-loaded DPPC liposomal large porous microparticles (RDLPMs) for treatment of A. baumannii-induced pneumonia. Methods: Novel RDLPMs were prepared by rotary evaporation and a freeze-drying method in this study. RDLPMs were evaluated by the particle size, electric potential, in vitro release, and particle size distribution. A rat model of A. baumannii-mediated pneumonia was established and used for pharmacodynamic evaluations. Results: The Re-loaded DPPC liposomes (RDLs) consisted of Re/DPPC (1:3, mol/mol) and DPPC/cholesterol (3:1, w/w), with a hydration time of 15 minutes. The RDLs had a high encapsulation efficiency of 69.8% ± 1.6%, a mean size of 191.5 ± 4.5 nm, and a high zeta potential of 12.4 ± 1.5 mV. The RDLPMs were composed of mannitol/large porous microparticles/RDLs (1:4:2, w/w/w) and had a loading efficiency of 2.20% ± 0.24%. The RDLPMs had an aerodynamic diameter (2.73 ± 0.65 µm), a good fluidity (28.30° ± 6.13°), and demonstrated high lung deposition (fine particle fraction = 43.33%). Surprisingly, while penicillin showed better microbial inhibition than the RDLPMs and Re groups in vitro, the RDLPMs were more effective in vivo. Conclusion: The RDLPMs showed good powder properties for pulmonary delivery. The RDLPMs may inhibit the nuclear factor kappa-B pathway and downregulate the expression of cytokines downstream of tumor necrosis factor-α and interleukin-1ß. As well as, RDLPMs demonstrated some antibacterial properties against A. baumannii bacteria. Re, when delivered in RDLPMs as a dry powder inhaler, is a promising substitute for antibiotics in the treatment of A. baumannii pneumonia.

8.
Thorac Cancer ; 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36630992

RESUMO

INTRODUCTION: This study investigated whether programmed death-ligand 1 (PD-L1) expression of circulating tumor cells (CTCs) in peripheral blood can serve as a predictive biomarker for immunotherapy efficacy in patients with advanced non-small-cell lung cancer (NSCLC). METHODS: We employed a negative enrichment method to isolate CTCs. We identified PD-L1 + CTCs as PD-L1+/4',6-diamidino-2-phenylindole (DAPI)+/CD45-circulating tumor cells through an immunofluorescence method. Tumor tissue PD-L1 expression was determined by immunohistochemical staining. The correlation between CTC PD-L1 expression and patients' prognostic features was estimated through the Kaplan-Meier method. RESULTS: CTCs released a higher detection rate of PD-L1 expression than tumor tissues (53.0% vs. 42.1%). No correlation was observed between them. Forty-nine NSCLC patients received anti-PD-1/PD-L1 immunotherapy (three with combined anti-PD-1/PD-L1 and cytotoxic T lymphocyte-associated antigen-4 (CTLA-4), two with four cycles of combined immune checkpoint inhibitors [ICIs] plus chemotherapy and ICI monotherapy for maintenance). Patients with PD-L1 expression on tissue or CTCs had a median progression-free survival (mPFS) of 5.6 months (n = 36, 95% confidence interval [CI] 3.6-7.5 months), significantly longer than those without PD-L1 detection (n = 9, mPFS of 1.4 months, 95% CI 1.3-1.5 months, log-rank p = 0.032). The multivariable Cox proportional-hazard model suggested that the tissue or CTC PD-L1 expression was associated with a lower risk of progression (hazard ratio 0.45, 95% CI 0.21-0.98, p = 0.043). CONCLUSIONS: CTCs and tumor tissues reveal heterogeneous expression of PD-L1 in NSCLC patients. Patients with baseline PD-L1 expression on CTCs or tissue showed prolonged mPFS and may help to identify the subsets of patients who potentially benefit from immunotherapy.

9.
J Pharm Biomed Anal ; 226: 115235, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36680806

RESUMO

Major chemical constituents in medicinal materials are often used as the marker compounds of traditional Chinese medicine (TCM) for treating various diseases. For spatholobi caulis (SPC), it contains a variety of flavones, phenolic acid esters, and lignans which exert many pharmacological effects. However, the absorption and permeability properties of these constituents of SPC are still unclear and require further investigation. Different types and major compounds of SPC were chosen as representative constituents to study their absorption and transepithelial transport characteristics in the human intestinal epithelium-like Caco-2 cell monolayer model. 35 constituents of SPC were evaluated by using ultra fast liquid chromatography combined with electrospray ionization triple quadrupole tandem mass spectrometry (UFLC-MS/MS) method, acetonitrile and water containing with 0.5 mM ammonium acetate were used as mobile phase, these analytes with good linear relationships (R2 was within 0.9967-0.9998), precision (CV values were less than 10.23 %, LLOQ was less than 13.69 %), accuracy (Mean of inter- and intra-day were within 85.02 %-111.61 % and 85.50-112.97 %, respectively) and stability (The mean was within 85.07 %-113.93 %), among which 16 analytes showed good permeability, 5 analytes were considered to be poorly permeable compounds, and the other 14 analytes were assigned for the moderately absorbed compounds in Caco-2 cell monolayer model. The further results showed that the absorption mechanism of 7 well absorbed compounds, 8-O-methylretusin (1), genistein (7), spasuberol B (16), naringenin (18), isoliquiritigenin (19), 4-hydroxy-3-methoxy cinnamic acid methyl ester (23) and (+)-epipinoresinol (31) in SPC was mainly passive diffusion, their bidirectional transport rate was correlated with the concentration and transport time. The chemical structures of these compounds could affect the permeability properties on the cell monolayer. This study demonstrated the utility of Caco-2 cell monolayer model for evaluating the absorption properties and initial mechanisms of compounds in SPC in vitro, and provided important basis for predicting oral bioavailability of SPC compounds.

10.
Cancer Res ; 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36607699

RESUMO

Pyroptosis is a type of programmed cell death characterized by the activation of inflammatory caspases and the cleavage of gasdermin proteins. Pyroptosis can suppress tumor development and induce anti-tumor immunity, and activating pyroptosis is a potential treatment strategy for cancer. To uncover approaches to harness the anti-cancer effects of pyroptosis, we aimed to identify regulators of pyroptosis in cancer. A CRISPR-Cas9 screen identified that loss of USP48, a deubiquitinating enzyme, significantly inhibited cell pyroptosis. USP48 promoted pyroptosis by stabilizing gasdermin E (GSDME). USP48 bound GSDME and removed K48-linked ubiquitination at positions K120 and K189. Clinical tissue testing confirmed that the expression of USP48 positively correlated with GSDME and pyroptosis-related factors. Single-cell sequencing showed that the functions of T cells and tumor-associated macrophages in the tumor microenvironment were inhibited after USP48 knockout. Finally, overexpression of USP48 enhanced the therapeutic efficacy of PD-1 inhibitors in tumors in mouse models. Together, these findings define a pyroptosis regulation pathway and indicate that pharmacological activation of USP48 may provide an effective strategy to sensitize cancer cells to pyroptosis and improve response to immunotherapy.

11.
Nano Lett ; 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36655867

RESUMO

Electrospun fibers have received wide attention in various fields ranging from the environment and healthcare to energy. However, nearly all electrospun fibers suffer from a pseudonanoscale diameter, resulting in fabricated membranes with a large pore size and limited separation performance. Herein, we report a novel strategy based on manipulating the equilibrium of stretch deformation and phase separation of electrospun jets to develop true-nanoscale fibers for effective selective separation. The obtained fibers present true-nanoscale diameters (∼67 nm), 1 order of magnitude less than those of common electrospun fibers, which endows the resultant membranes with remarkable nanostructural characteristics and separation performances in areas of protective textiles (waterproofness of 113 kPa and breathability of 4.1 kg m-2 d-1), air filtration (efficiency of 99.3% and pressure drop of 127.4 Pa), and water purification (flux of 81.5 kg m-2 h-1 and salt rejection of 99.94%). This work may shed light on developing high-performance separation materials for various applications.

12.
Epilepsia Open ; 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36650667

RESUMO

OBJECTIVE: Performing long-term video-electroencephalographic monitoring (LTVEM) to obtain the ictal electroencephalogram (EEG) is important for presurgical evaluation. This study aimed to investigate the safety and efficacy of our protocol developed at Peking University First Hospital (PUFH) for rapid withdrawal of anti-seizure medications (ASMs) during LTVEM to induce seizures in children with drug-resistant epilepsy (DRE) exhibiting non-daily seizures. METHODS: Children with DRE who followed the PUFH protocol for rapid withdrawal of ASMs during LTVEM between 2018 and 2021 were enrolled. The occurrence of seizures, number of ASMs withdrawn, seizure onset time after ASM tapering initiation, changes in interictal epileptiform discharge (IED), and adverse events were evaluated during LTVEM. RESULTS: Among 80 children evaluated in this study, seizures were induced successfully in 72 (90%) children. Furthermore, no change in IED sites was observed in these 72 children following the initiation of ASM tapering while 2 children exhibited secondary bilateral tonic-clonic seizures. The median time from ASM tapering initiation to the onset of the first seizure was found to be 3 days (2-4), while the median number of ASMs withdrawn was 2 (1-2). Finally, 66 children (91.7%) had habitual seizures while 6 children had non-habitual seizure semiology. SIGNIFICANCE: The PUFH protocol can be used for the rapid withdrawal of ASMs during LTVEM in children with DRE. Using this protocol, ictal EEG patterns can be obtained in a relatively short time for most patients with fewer adverse effects during LTVEM, which may provide meaningful electro-clinical information for presurgical evaluation.

13.
ACS Omega ; 8(1): 1375-1388, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36643466

RESUMO

To investigate the effect of concentration of N2 and CO2 (0, 10, 20, 30, 40, and 50%) on the flame propagation characteristics of CH4/air premixed gases with stoichiometric ratios in variable cross-section ducts, experiments were conducted in four combinations of ducts at initial conditions of 298 K and 1 atm. The results show that the flame propagation velocity, propagation time, and overpressure are greater in the suddenly contracted duct than in the suddenly expanded duct if the dimensions of the ducts are kept constant. However, an increase in inert gas concentration leads to a decrease in flame propagation speed, an increase in flame propagation time, and changes in flame structure and pressure. "Tulip" flames appeared when a duct with a cross section of 100 mm × 100 mm was combined with a duct with a cross section of 70 mm × 70 mm, whether N2 or CO2 was added or what its concentration was. However, when a duct with a cross section of 140 mm × 140 mm was combined with a 70 mm × 70 mm duct, a "tulip" flame is formed only at a CO2 concentration of 50%. As the concentration of inert gas increases, the explosion pressure first decreases and then stabilizes, while the rate of pressure increase showed a monotonically decreasing trend. The explosion pressure is minimized when the concentration of CO2 and N2 is 30 and 40%, respectively.

14.
Sci Total Environ ; 864: 161011, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36549517

RESUMO

Scanty attention has been paid to augmenting the denitrification performance of polluted lake water by adding mix-cultured aerobic denitrifying bacterial communities (Mix-CADBCs). In this study, to solve the serious problem of nitrogen pollution in lake water bodies, aerobic denitrifying bacteria were added to lake water to enhance the nitrogen and carbon removal ability. Three Mix-CADBCs were isolated from lake water and they could remove >94 % of total nitrogen and dissolved organic carbon, respectively. The balance of nitrogen analysis shown that >70 % of the initial nitrogen was converted to gaseous nitrogen, and <11 % of the initial nitrogen was converted into microbial biomass. The batch experiments indicated that three Mix-CADBCs could perform denitrification under various conditions. According to the results of nirS-type sequencing, the Hydrogenophaga sp., Prosthecomicrobium sp., and Pseudomonas sp. were dominated genera of three Mix-CADBCs. The analysis of network indicated Pseudomonas I.Bh25.14 and Vogsella LIG4 were correlated with the removal of total nitrogen (TN) and dissolved organic carbon in the Mix-CADBCs. Compared with lake raw water, the addition of three Mix-CADBCs could promote the denitrification capacity (the removal efficiencies of TN > 78.72 %), microbial growth (optical density increased by 0.015-0.138 and the total cell count increased by 2 times), and organic degradation ability (the removal efficiency chemical oxygen demand >38 %) of lake water. In general, the findings of this study demonstrated that Mix-CADBCs could provide a new perspective for biological treatment lake water body.


Assuntos
Desnitrificação , Lagos , Matéria Orgânica Dissolvida , Água , Nitrogênio
15.
Biomed Pharmacother ; 158: 114133, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36521243

RESUMO

Endoplasmic reticulum stress (ERS)-related autophagy is involved in the occurrence and development of ulcerative colitis (UC). Therefore, regulating ERS-related autophagy is a potential therapeutic target for the treatment of UC. Jianpi-Qingchang (JPQC) decoction, consisting of nine Chinese herbal medicines, is used to treat patients with UC. However, its mechanism of action has not been completely elucidated. Here, we aimed to reveal the therapeutic effects and mechanisms of JPQC in UC. We established a colitis model using dextran sulfate sodium (DSS) and an ERS model using thapsigargin (Tg) and administered JPQC. We systematically examined ERS-related autophagy associated protein expression, inflammatory cytokines, apoptotic cells, and autophagic flux. Moreover, the cellular ultrastructure was observed via transmission electron microscopy (TEM). We found that JPQC reduced disease activity index (DAI) scores, counteracted colonic tissue damage, decreased the number of autophagosomes, inhibited proinflammatory cytokines, enhanced anti-inflammatory cytokines, and dampened ERS-related autophagy associated protein gene expression.


Assuntos
Colite Ulcerativa , Colite , Humanos , Animais , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/tratamento farmacológico , Colite Ulcerativa/metabolismo , Colite/tratamento farmacológico , Colo , Células Epiteliais , Citocinas/metabolismo , Autofagia , Estresse do Retículo Endoplasmático , Sulfato de Dextrana/toxicidade , Modelos Animais de Doenças
16.
Talanta ; 253: 123954, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36162188

RESUMO

Design of oligonucleotide probe-based isothermal amplification with the ability to identify miRNA biomarkers is crucial for molecular diagnostics. Herein, we engineered a miRNA-21 responsive G-quadruplex-deficient precursor hairpin probe (PHP) to achieve dual-mode detection of fluorescent signal and colorimetric signal. Due to lack of complete G-quadruplex sequence, PHP becomes shorter in length, lower background signal and less interference. Based on the polymerase-driven amplification mechanism, in the presence of miRNAs, two simultaneous amplification reaction processes will occur in PHP: miRNA-based amplification process and endogenous amplification process along the 3' end. Due to the positional difference between the starting points of the two amplification processes, the orderly and efficient occurrence of the two amplification processes can be achieved. Based on an interesting concept, PHP can achieve high detection performance with only simple amplification cycles. In such a way, the detection limits for fluorescence and colorimetry were 2.93 fM and 8.81 fM, which would cover most of clinical qualitative and quantitative needs. Thus, the accurate quantitative and visual miRNA detection technology based on PHP is beneficial to carry out extensive disease screening and treatment monitoring in various complex occasions.


Assuntos
MicroRNAs , MicroRNAs/genética
17.
Cities ; 132: 104104, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36407935

RESUMO

The COVID-19 pandemic has brought huge challenges to sustainable urban and community development. Although some recovery signals and patterns have been uncovered, the intra-city recovery process remains underexploited. This study proposes a comprehensive approach to quantify COVID-19 recovery leveraging fine-grained human mobility records. Taking Wuhan, a typical COVID-19 affected megacity in China, as the study area, we identify accurate recovery phases and select appropriate recovery functions in a data-driven manner. We observe that recovery characteristics regarding duration, amplitude, and velocity exhibit notable differences among urban blocks. We also notice that the recovery process under a one-wave outbreak lasts at least 84 days and has an S-shaped form best fitted with four-parameter Logistic functions. More than half of the recovery variance can be well explained and estimated by common variables from auxiliary data, including population, economic level, and built environments. Our study serves as a valuable reference that supports data-driven recovery quantification for COVID-19 and other crises.

18.
Sci Total Environ ; 864: 161105, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36566853

RESUMO

Microbial communities composed of few abundant and many rare species are widely involved in the biogeochemical cycles of elements. Yet little is known about the ecological roles of rare taxa in antimony (Sb) contaminated groundwater. Groundwater samples were collected along an Sb concentration gradient in the Xikuangshan antimony mine area and subjected to high through-put sequencing of 16S rRNA genes to investigate the bacterial communities. Results suggested that both abundant and rare sub-communities were dominated by Betaproteobacteria, Gammaproteobacteria, and Alphaproteobacteria, whereas rare sub-communities showed higher alpha-diversities. Multivariate analysis showed that both the abundant and rare taxa were under the stress of Sb, but the impact on rare taxa was greater. Nitrate explained a large part for the variation of the abundant sub-communities, indicating the critical role of nitrate for their activities under anoxic conditions. In contrast, bicarbonate significantly impacted rare sub-communities, suggesting their potential autotrophic characteristics. To further explore the role of rare taxa in the communities and the mechanism of affecting the community composition, a network was constructed to display the co-occurrence pattern of bacterial communities. The rare taxa contributed most of the network nodes and served as keystone species to maintain the stability of community. Abiotic factors (mainly Sb and pH) and bacterial interspecific interactions (interactions between keystone species and other bacterial groups) jointly affect the community dynamics. Functional prediction was performed to further reveal the ecological function of rare taxa in the Sb-disturbed groundwater environment. The results indicated that the rare taxa harbored much more diverse functions than their abundant counterparts. Notably, elevated Sb concentration promoted some potential autotrophic functions in rare taxa such as the oxidation of S-, N-, and Fe(II)-compounds. These results offer new insights into the roles of rare species in elemental cycles in the Sb-impacted groundwater.


Assuntos
Arsênio , Água Subterrânea , Antimônio/análise , Nitratos/análise , RNA Ribossômico 16S , Arsênio/análise , Bactérias , Água Subterrânea/química
19.
Front Immunol ; 13: 1059376, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36466812

RESUMO

ADAM17 is a member of the a disintegrin and metalloproteinase (ADAM) family of transmembrane proteases involved in the shedding of some cell membrane proteins and regulating various signaling pathways. More than 90 substrates are regulated by ADAM17, some of which are closely relevant to tumor formation and development. Besides, ADAM17 is also responsible for immune regulation and its substrate-mediated signal transduction. Recently, ADAM17 has been considered as a major target for the treatment of tumors and yet its immunomodulatory roles and mechanisms remain unclear. In this paper, we summarized the recent understanding of structure and several regulatory roles of ADAM17. Importantly, we highlighted the immunomodulatory roles of ADAM17 in tumor development, as well as small molecule inhibitors and monoclonal antibodies targeting ADAM17.


Assuntos
Antineoplásicos Imunológicos , Neoplasias , Humanos , Imunomodulação , Metaloproteases , Anticorpos Monoclonais , Proteína ADAM17
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