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1.
BMC Genomics ; 21(1): 53, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31948407

RESUMO

BACKGROUND: Ca2+ played as a ubiquitous secondary messenger involved in plant growth, development, and responses to various environmental stimuli. Calcium-dependent protein kinases (CDPK) were important Ca2+ sensors, which could directly translate Ca2+ signals into downstream phosphorylation signals. Considering the importance of CDPKs as Ca2+ effectors for regulation of plant stress tolerance and few studies on Brachypodium distachyon were available, it was of interest for us to isolate CDPKs from B. distachyon. RESULTS: A systemic analysis of 30 CDPK family genes in B. distachyon was performed. Results showed that all BdCDPK family members contained conserved catalytic Ser/Thr protein kinase domain, autoinhibitory domain, and EF-hand domain, and a variable N-terminal domain, could be divided into four subgroup (I-IV), based upon sequence homology. Most BdCDPKs had four EF-hands, in which EF2 and EF4 revealed high variability and strong divergence from EF-hand in AtCDPKs. Synteny results indicated that large number of syntenic relationship events existed between rice and B. distachyon, implying their high conservation. Expression profiles indicated that most of BdCDPK genes were involved in phytohormones signal transduction pathways and regulated physiological process in responding to multiple environmental stresses. Moreover, the co-expression network implied that BdCDPKs might be both the activator and the repressor involved in WRKY transcription factors or MAPK cascade genes mediated stress response processes, base on their complex regulatory network. CONCLUSIONS: BdCDPKs might play multiple function in WRKY or MAPK mediated abiotic stresses response and phytohormone signaling transduction in B. distachyon. Our genomics analysis of BdCDPKs could provide fundamental information for further investigation the functions of CDPKs in integrating Ca2+ signalling pathways in response to environments stresses in B. distachyon.

2.
Phys Chem Chem Phys ; 22(2): 914-918, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31845940

RESUMO

As a representative in-plane anisotropic two-dimensional (2D) material, germanium monoselenide (GeSe) has attracted considerable attention recently due to its various in-plane anisotropic material properties originating from the low symmetry of a puckered honeycomb structure. Although there have been plenty of reports on the in-plane anisotropic vibrational, electrical and optical properties of GeSe, the strain effect on those appealing anisotropies is still under exploration. Here we report a systematic first-principles computational investigation of strain-engineering of the anisotropic electronic properties of GeSe monolayers. We found that the anisotropic ratio of the effective mass and mobility of charge carriers (electrons and holes) of GeSe along two principle axes can be controlled by using simple strain conditions. Notably, the preferred conducting direction of GeSe can be even rotated by 90° under an appropriate uniaxial strain (>5%). Such effective strain modulation of the electronic anisotropy of GeSe monolayers provides them abundant opportunities for future mechanical-electronic devices.

3.
Biomed Res Int ; 2019: 8530273, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31687402

RESUMO

In recent years, many studies have shown that recombinant adenovirus live vector-based vaccines are a promising novel vaccine candidate against virus infection. Therefore, in this study, a new type of recombinant adenovirus expressing the spike (S) protein of porcine epidemic diarrhea virus (PEDV), rAd-PEDV-S, was generated, and its characteristics were determined. Then, its efficacy as a vaccine candidate was evaluated in 4-week-old pigs. The results showed that the S protein could be well expressed at a high level in rAd-PEDV-S-infected cells and that the viral titers could reach 1011 PFU/mL. Further animal experimental results showed that rAd-PEDV-S elicited a significant PEDV-specific humoral immune response after vaccination (P < 0.05). In addition, rAd-PEDV-S provided partial protection for pigs against the highly virulent PEDV challenge. The results presented in this study indicate that the adenovirus vector can be used as a vaccine delivery vector for the development of a PEDV vaccine and is a promising novel vaccine candidate for future prevention and control of porcine epidemic diarrhea (PED), but its efficacy still needs to be improved in the future.

4.
Viruses ; 11(8)2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31394868

RESUMO

Tumor suppressor protein p53 (p53) is a master transcription factor that plays key roles in cell cycle arrest, apoptosis, senescence, and metabolism, as well as regulation of innate immunity during virus infection. In order to facilitate their replication and spreading, viruses have evolved to manipulate p53 function through different strategies, with some requiring active p53 while others demand reduction/inhibition of p53 activity. However, there are no clear-cut reports about the roles of p53 during the infection of foot-and-mouth disease virus (FMDV), the causative agent of a highly contagious foot-and-mouth disease (FMD) of cloven-hoofed animals. Here we showed that p53 level was dynamically regulated during FMDV infection, being degraded at the early infection stage but recovered to the basal level at the late stage. Cells depleted of p53 showed inhibited FMDV replication and enhanced expression of the immune-related genes, whereas overexpression of p53 didn't affect the viral replication. Viral challenge assay with p53 knockout mice obtained similar results, with viral load decreased, histopathological changes alleviated, and lifespan extended in the p53 knockout mice. Together, these data demonstrate that basal level p53 is required for efficient FMDV replication by suppressing the innate immunity.

5.
Int J Mol Sci ; 20(17)2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31450734

RESUMO

GT factors play critical roles in plant growth and development and in response to various environmental stimuli. Considering the new functions of GT factors on the regulation of plant stress tolerance and seeing as few studies on Brachypodium distachyon were available, we identified GT genes in B. distachyon, and the gene characterizations and phylogenies were systematically analyzed. Thirty-one members of BdGT genes were distributed on all five chromosomes with different densities. All the BdGTs could be divided into five subfamilies, including GT-1, GT-2, GTγ, SH4, and SIP1, based upon their sequence homology. BdGTs exhibited considerably divergent structures among each subfamily according to gene structure and conserved functional domain analysis, but the members within the same subfamily were relatively structure-conserved. Synteny results indicated that a large number of syntenic relationship events existed between rice and B. distachyon. Expression profiles indicated that the expression levels of most of BdGT genes were changed under abiotic stresses and hormone treatments. Moreover, the co-expression network exhibited a complex regulatory network between BdGTs and BdWRKYs as well as that between BdGTs and BdMAPK cascade gene. Results showed that GT factors might play multiple functions in responding to multiple environmental stresses in B. distachyon and participate in both the positive and negative regulation of WRKY- or MAPK-mediated stress response processes. The genome-wide analysis of BdGTs and the co-regulation network under multiple stresses provide valuable information for the further investigation of the functions of BdGTs in response to environment stresses.


Assuntos
Brachypodium/genética , Biologia Computacional , Perfilação da Expressão Gênica , Genômica , Reguladores de Crescimento de Planta/genética , Estresse Fisiológico/genética , Biologia Computacional/métodos , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica de Plantas , Redes Reguladoras de Genes , Genes de Plantas , Genômica/métodos , Filogenia , Sintenia , Transcriptoma
6.
Virology ; 535: 162-170, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31306911

RESUMO

MicroRNAs (miRNAs) play important regulatory roles during interactions between virus pathogens and host cells, but whether and how they work in the case of foot-and-mouth disease virus (FMDV) is less understood. Based on a microarray-based miRNA profiling in the porcine kidney cell line PK-15, we identified 36 differentially expressed host miRNAs at the early stage of FMDV infection, among which miR-1307 was significantly induced. Functional characterization demonstrated that miR-1307 attenuated FMDV replication. Further experiments proved that miR-1307 specifically promoted the degradation of the viral structural protein VP3 indirectly through proteasome pathway. Moreover, innate immune signaling was activated and expression of immune responsive genes was significantly enhanced in the miR-1307-overexpressing clones. Together, our data demonstrated that miR-1307 suppresses FMDV replication by destabilizing VP3 and enhancing host immune response. Importantly, subcutaneous injection of miR-1307 agomir delayed the FMDV-induced lethality in suckling mice, exhibiting its therapeutic potential to control foot-and-mouth disease (FMD).

7.
Appl Microbiol Biotechnol ; 103(8): 3367-3379, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30888465

RESUMO

Many recent studies have shown that flagellin fused to heterologous antigens can induce significantly enhanced humoral and cellular immune responses through its adjuvant activity. Therefore, in this study, two key B cell epitopes and a truncated VP1 (ΔVP1) protein from foot-and-mouth disease virus (FMDV) were expressed as flagellin fusion proteins in different patterns. Specifically, ΔVP1 and two duplicates of two key B cell epitopes (2×B1B2) were fused separately to the C-terminus of flagellin with a universal exogenous T cell epitope to construct FT (Flagellin-Truncated VP1) and FME (Flagellin-Multiple Epitopes). In addition, the D3 domain of flagellin was replaced by ΔVP1 in FME, yielding FTME (Flagellin-Truncated VP1-Multiple Epitopes). The immunogenicity and protective efficacy of the three fusion proteins as novel FMDV vaccine candidates were evaluated. The results showed that FT, FME, and FTME elicited significant FMDV-specific IgG responses at 10 µg/dose compared with the mock group (P < 0.05), with FTME producing the highest response. No significant differences in the antibody response to FTME were observed between different immunization routes or among adjuvants (ISA-206, poly(I·C), MPLA, and CpG-ODN) in mice. In addition, at 30 µg/dose, all three fusion proteins significantly induced neutralizing antibody production and upregulated the levels of some cytokines, including TNF-α, IFN-γ, and IL-12, in guinea pigs. Importantly, all three fusion proteins provided effective protective immunity against FMDV challenge in guinea pigs, though different protection rates were found. The results presented in this study indicate that the FTME fusion protein is a promising novel vaccine candidate for the future prevention and control of foot-and-mouth disease.


Assuntos
Flagelina/imunologia , Vírus da Febre Aftosa/imunologia , Febre Aftosa/prevenção & controle , Vacinação/métodos , Vacinas Virais/imunologia , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Antígenos Virais/genética , Antígenos Virais/imunologia , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/imunologia , Citocinas/sangue , Epitopos de Linfócito B/genética , Epitopos de Linfócito B/imunologia , Epitopos de Linfócito T/genética , Epitopos de Linfócito T/imunologia , Feminino , Flagelina/genética , Vírus da Febre Aftosa/genética , Cobaias , Masculino , Camundongos Endogâmicos BALB C , Proteínas Recombinantes de Fusão/administração & dosagem , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Vacinas Virais/administração & dosagem , Vacinas Virais/genética
8.
Vet Microbiol ; 230: 278-282, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30827401

RESUMO

Although highly virulent GII-genotype PEDV strains have become pandemic in the swine population worldwide, little is known about the differences in immunogenicity and cross-protective efficacy between the GIIa and GIIb subgenotypes. Hence, in the present study, we vaccinated suckling piglets with GIIa (CH/HBXT/2018) and GIIb (CH/HNPJ/2017) PEDV strain-based inactivated vaccine candidates and compared their immunogenicity and cross-protective efficacy. The results showed that both vaccine candidates induced high levels of PEDV-specific IgG antibodies and IFN-γ and reduced the levels of neutralizing antibodies at 21 dpv in suckling piglets. The GIIa-based inactivated vaccine protected all piglets (8/8) against virulent homologous and heterologous virus challenge, while the GIIb strain-based inactivated vaccine protected only 2/4 and 1/4 piglets against virulent homologous and heterologous virus challenge, respectively. Furthermore, antibodies against the GIIa and GIIb strains cross-reacted and cross-neutralized both strains in vitro. Taken together, the data presented in this study indicate that GIIa strain-based inactivated vaccine candidates are more promising than GIIb-based candidates for the development of an effective vaccine against the current highly virulent pandemic PEDV strains.


Assuntos
Anticorpos Antivirais/sangue , Infecções por Coronavirus/veterinária , Proteção Cruzada/imunologia , Imunogenicidade da Vacina , Doenças dos Suínos/prevenção & controle , Vacinas Virais/imunologia , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , Técnicas de Cultura de Células , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/prevenção & controle , Genótipo , Imunoglobulina G/sangue , Interferon gama/imunologia , Vírus da Diarreia Epidêmica Suína/genética , Vírus da Diarreia Epidêmica Suína/patogenicidade , Suínos , Doenças dos Suínos/imunologia , Vacinas de Produtos Inativados/imunologia , Vacinas Virais/administração & dosagem , Eliminação de Partículas Virais
9.
Arch Virol ; 164(5): 1287-1295, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30859476

RESUMO

Since 2010, continual outbreaks of highly virulent variants of porcine epidemic diarrhea virus (PEDV) belonging to genotype GII have led to serious economic losses for the Chinese swine industry. To better understand the biological characteristics and pathogenicity of the current prevalent Chinese PEDV field strains, in this study, a highly virulent Chinese genotype GIIa PEDV strain, CH/HBXT/2018, was isolated and serially propagated using Vero cells. Sequencing and phylogenetic analysis showed that strain CH/HBXT/2018 contained novel insertion and deletion mutations in the S gene region relative to the classical strain and belonged to the genotype GIIa, similar to other recently isolated PEDV strains from China and the United States. Pig infection studies indicated that the CH/HBXT/2018 strain was highly virulent in suckling piglets, and the median pig diarrhea dose (PDD50) was 8.63 log10PDD50/3 mL at 7 days postinfection (DPI). The results of the present study are important for future PEDV challenge studies and the development of new PEDV vaccines based on prevalent field strains for the prevention and control of PED in China.


Assuntos
Infecções por Coronavirus/veterinária , Vírus da Diarreia Epidêmica Suína/genética , Vírus da Diarreia Epidêmica Suína/patogenicidade , Glicoproteína da Espícula de Coronavírus/genética , Animais , Linhagem Celular , China , Infecções por Coronavirus/virologia , Surtos de Doenças , Genótipo , Mutagênese Insercional/genética , Filogenia , Vírus da Diarreia Epidêmica Suína/classificação , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Deleção de Sequência/genética , Suínos , Doenças dos Suínos/virologia , Células Vero , Vacinas Virais/imunologia , Virulência/genética
10.
Risk Anal ; 39(3): 553-570, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30278114

RESUMO

Recollection bias (RB) refers to the phenomenon whereby after an adverse event people report that their risk assessment about a similar future event is presently no higher than their recollection of their pre-event risk assessment. While previous research has outlined this theoretical construct and generated important empirical findings, there were some limitations. We design and employ a new national representative survey to address these limitations in this study. We examine the existence and persistence of RB among the general public in the context of a number of domestic and international terrorist attacks. We further examine the socioeconomic and political base of RB and the influences of RB on a wide range of citizens' counterterrorism policy preferences. Our data analyses reveal strong evidence showing the occurrence of RB and its persistence across various forms of terrorism risk. With regard to the socioeconomic and political base, we find that females, older people, political conservatives, and Republicans are less likely to be subject to RB. For the effects of RB on public counterterrorism policy preferences, our analyses demonstrate that this bias significantly dampens public support for a wide range of preventive policy measures and government anti-terrorism spending. Overall, our study, based on a national representative sample and an extended survey design, provides robust evidence of RB in terrorism risk assessment, and adds further evidence to support the idea that RB is likely a generalizable phenomenon. Implications and suggestions for future research are discussed in the conclusion.

11.
Virus Res ; 259: 18-27, 2019 01 02.
Artigo em Inglês | MEDLINE | ID: mdl-30342075

RESUMO

Since October 2010, severe porcine epidemic diarrhea (PED) outbreaks caused by highly virulent PED virus (PEDV) strains have occurred continuously in the Chinese pig population and caused considerable economic losses. Although PEDV vaccines based on classical PEDV strains have been widely used in China in recent years, the morbidity and mortality in piglets remain high. Therefore, virulent genotype GII PEDV strains that are prevalent in the field should be isolated and used to develop next-generation vaccines. In the present study, a Chinese virulent genotype GIIb PEDV strain, CH/HNPJ/2017, was serially propagated in Vero cells for up to 90 passages. The S genes contained typical insertions and deletions that were also found in other recently isolated highly virulent PEDV strains from China and other countries and had two neighboring unique insertion mutations, which resulted in four amino acid changes in the S1 region of passages P10 and P60. Pig infection studies revealed that the CH/HNPJ/2017 strain was highly virulent in piglets, and the median pig diarrhea dose (PDD50) was 7.68 log10PDD50/3 mL. Furthermore, the cell-adapted CH/HNPJ/2017 strain elicited potent serum IgG and neutralizing antibody responses in immunized pigs when it was used as an inactivated vaccine candidate. In addition, the pigs that received the experimental inactivated vaccines were partially protected (3/5) against subsequent viral challenge. In brief, these data indicate that the CH/HNPJ/2017 strain is a promising candidate for developing a safe and effective PEDV vaccine in the future.


Assuntos
Infecções por Coronavirus/veterinária , Genótipo , Vírus da Diarreia Epidêmica Suína/genética , Doenças dos Suínos/virologia , Animais , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , Linhagem Celular , Diarreia/veterinária , Interações Hospedeiro-Patógeno/imunologia , Testes de Neutralização , Filogenia , Vírus da Diarreia Epidêmica Suína/classificação , Vírus da Diarreia Epidêmica Suína/imunologia , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Glicoproteína da Espícula de Coronavírus/genética , Glicoproteína da Espícula de Coronavírus/imunologia , Suínos , Doenças dos Suínos/imunologia , Doenças dos Suínos/patologia , Doenças dos Suínos/prevenção & controle , Vacinas de Produtos Inativados/imunologia , Células Vero , Vacinas Virais/imunologia , Virulência
12.
ACS Appl Mater Interfaces ; 10(38): 32067-32077, 2018 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-30183246

RESUMO

The inhibition of the rapid recombination of photochemical charges at heterointerfaces and the promotion of transfer and extraction are central to photoelectric conversion in solar cells. With the use of Cu4Bi4S9 nanoribbons-graphene sheets (CBS-GSs) as a hybrid photosensitive layer and the growth of ZnO nanowires on Zn2SnO4 nanowires (ZTO-ZnO) for the electron transfer layer, a flexible solar cell ZTO-ZnO/CBS-GSs was prepared on a stainless steel mesh. In this study, time-resolved fluorescence spectroscopy (TFS) and transient surface photovoltage (TPV) were used to describe the transition and transport process for the photogenerated carriers. With a high fill factor (0.76) and improved electron mobility for the ZTO-ZnO nanostructure, a high photoelectric conversion efficiency of 11.6% was obtained, which was evidently higher than that of a ZTO/CBS-GSs cell (9.2%) and a ZnO nanoparticles/CBS-GSs cell (3.9%). The efficient dissociation of photogenerated carriers at CBS-GSs interfaces, rapid transfer of free electrons in the ZTO-ZnO system, and fast extraction of holes from the selective NiO layer with an optimized architecture led to a superior performance. The charge recombination at the interface can be determined by electrochemical impedance spectroscopy (EIS). Active exploration via inevitable competition between charge separation and recombination (TFS, TPV, EIS, etc.) can provide insights into the entire dynamic process and a separation mechanism for photoinduced carriers, which can also promote the application of flexible thin-film solar cells.

13.
PLoS One ; 13(7): e0200081, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29979724

RESUMO

Foot-and-mouth disease virus (FMDV) is a single-stranded positive RNA virus that belongs to the family Picornaviridae. FMDV infects cloven-hoofed animals, such as pigs, sheep, goats, cattle and diverse wildlife species, and remains a major threat to the livestock industry worldwide. In this study, a transcriptome analysis of whole blood from pigs infected with FMDV was performed using the paired-end Illumina sequencing technique to understand the interactions between the pathogen and its host cells. During infection with FMDV, a total of 120 differentially expressed genes (DEGs) were identified, including 110 up-regulated genes and 10 down-regulated genes. To further investigate the DEGs involved in interactions between the virus and its host, gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment were conducted. GO annotation indicated that a number of DEGs were enriched in categories involved in host-virus interactions, such as response to stimulus, immune system process and regulation of biological process. KEGG enrichment analysis indicated that the DEGs were primarily involved in the ribosome signaling pathway and immune-related signaling pathways. Ten DEGs, including the immune-related genes BTK1, C1QB, TIMD4 and CXCL10, were selected and validated using quantitative PCR, which showed that the expression patterns of these genes are consistent with the results of the in silico expression analysis. In conclusion, this study presents the first transcriptome analysis of pig whole blood cells infected with FMDV, and the results obtained in this study improve our understanding of the interactions between FMDV and host cells as well as the diagnosis and control of FMD.


Assuntos
Células Sanguíneas/metabolismo , Células Sanguíneas/virologia , Vírus da Febre Aftosa/fisiologia , Perfilação da Expressão Gênica , Sequenciamento de Nucleotídeos em Larga Escala , Animais , Marcadores Genéticos/genética , Anotação de Sequência Molecular , Suínos
14.
Expert Rev Vaccines ; 17(4): 335-349, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29580106

RESUMO

INTRODUCTION: Bacterial flagellin, as a pathogen-associated molecular pattern (PAMP), can activate both innate and adaptive immunity. Its unique structural characteristics endow an effective and flexible adjuvant activity, which allow the design of different types of vaccine strategies to prevent various diseases. This review will discuss recent progress in the mechanism of action of flagellin and its prospects for use as a vaccine adjuvant. AREAS COVERED: Herein we summarize various types of information related to flagellin adjuvants from PubMed, including structures, signaling pathways, natural immunity, and extensive applications in vaccines, and it discusses the immunogenicity, safety, and efficacy of flagellin-adjuvanted vaccines in clinical trials. EXPERT COMMENTARY: It is widely accepted that as an adjuvant, flagellin can induce an enhanced antigen-specific immune response. Flagellin adjuvants will allow more effective flagellin-based vaccines to enter clinical trials. Furthermore, vaccine formulations containing PAMPs are crucial to exert the maximum potential of vaccine antigens. Therefore, combinations of flagellin-adjuvanted vaccines with other adjuvants that act in a synergistic manner, particularly TLR ligands, represent a promising method for tailoring targeted vaccines to meet specific requirements.


Assuntos
Adjuvantes Imunológicos/administração & dosagem , Flagelina/administração & dosagem , Vacinas/administração & dosagem , Imunidade Adaptativa/imunologia , Animais , Antígenos/imunologia , Flagelina/imunologia , Humanos , Imunidade Inata/imunologia , Transdução de Sinais/imunologia , Vacinas/imunologia
15.
Biomed Res Int ; 2017: 7658970, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29119112

RESUMO

Improving vaccine immunogenicity by targeting antigens to dendritic cells has recently emerged as a new design strategy in vaccine development. In this study, the VP1 gene of foot-and-mouth disease virus (FMDV) serotype A was fused with the gene encoding human immunodeficiency virus (HIV) membrane glycoprotein gp120 or C2-V3 domain of hepatitis C virus (HCV) envelope glycoprotein E2, both of which are DC-SIGN-binding glycoproteins. After codon optimization, the VP1 protein and the two recombinant VP1-gp120 and VP1-E2 fusion proteins were expressed in Sf9 insect cells using the insect cell-baculovirus expression system. Western blotting showed that the VP1 protein and two recombinant VP1-gp120 and VP1-E2 fusion proteins were correctly expressed in the Sf9 insect cells and had good reactogenicity. Guinea pigs were then immunized with the purified proteins, and the resulting humoral and cellular immune responses were analyzed. The VP1-gp120 and VP1-E2 fusion proteins induced significantly higher specific anti-FMDV antibody levels than the VP1 protein and stronger cell-mediated immune responses. This study provides a new perspective for the development of novel FMDV subunit vaccines.


Assuntos
Proteínas do Capsídeo , Vírus da Febre Aftosa , Proteína gp120 do Envelope de HIV , Imunogenicidade da Vacina , Proteínas Recombinantes de Fusão , Vacinas Virais , Animais , Proteínas do Capsídeo/biossíntese , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/imunologia , Proteínas do Capsídeo/farmacologia , Moléculas de Adesão Celular , Vírus da Febre Aftosa/genética , Vírus da Febre Aftosa/imunologia , Cobaias , Proteína gp120 do Envelope de HIV/biossíntese , Proteína gp120 do Envelope de HIV/genética , Proteína gp120 do Envelope de HIV/imunologia , Proteína gp120 do Envelope de HIV/farmacologia , HIV-1/genética , Hepacivirus , Lectinas Tipo C , Receptores de Superfície Celular , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Proteínas Recombinantes de Fusão/farmacologia , Células Sf9 , Spodoptera , Vacinas Virais/biossíntese , Vacinas Virais/genética , Vacinas Virais/farmacologia
16.
Virol J ; 14(1): 194, 2017 10 10.
Artigo em Inglês | MEDLINE | ID: mdl-29017599

RESUMO

BACKGROUND: Large-scale outbreaks of porcine epidemic diarrhea (PED) have re-emerged in China in recent years. However, little is known about the genetic diversity and molecular epidemiology of field strains of PED virus (PEDV) in China in 2016-2017. To address this issue, in this study, 116 diarrhea samples were collected from pig farms in 6 Chinese provinces in 2016-2017 and were detected using PCR for main porcine enteric pathogens, including PEDV, porcine deltacoronavirus (PDCoV), porcine transmissible gastroenteritis virus (TGEV) and porcine kobuvirus (PKV). In addition, the complete S genes from 11 representative PEDV strains were sequenced and analyzed. RESULTS: PCR detection showed that 52.6% (61/116) of these samples were positive for PEDV. Furthermore, sequencing results for the spike (S) genes from 11 of the epidemic PEDV strains showed 93-94% nucleotide identity and 92-93% amino acid identity with the classical CV777 strain. Compared with the CV777 vaccine strain, these strains had an insertion (A133), a deletion (G155), and a continuous 4-amino-acid insertion (56NNTN59) in the S1 region. Phylogenetic analysis based on the S gene indicated that the 11 assessed PEDV strains were genetically diverse and clustered into the G2 group. These results demonstrate that the epidemic strains of PEDV in China in 2016-2017 are mainly virulent strains that belong to the G2 group and genetically differ from the vaccine strain. Importantly, this is the first report that the samples collected in Hainan Province were positive for PEDV (59.2%, 25/42). CONCLUSIONS: To our knowledge, this article presents the first report of a virulent PEDV strain isolated from Hainan Island, China. The results of this study will contribute to the understanding of the epidemiology and genetic characteristics of PEDV in China.


Assuntos
Infecções por Coronavirus/veterinária , Variação Genética , Filogenia , Vírus da Diarreia Epidêmica Suína/classificação , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Glicoproteína da Espícula de Coronavírus/genética , Animais , China/epidemiologia , Coronavirus/isolamento & purificação , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Surtos de Doenças , Kobuvirus/isolamento & purificação , Epidemiologia Molecular , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/veterinária , Infecções por Picornaviridae/virologia , Reação em Cadeia da Polimerase , Vírus da Diarreia Epidêmica Suína/genética , Suínos , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/virologia , Vírus da Gastroenterite Transmissível/isolamento & purificação
17.
Vet Res ; 48(1): 61, 2017 10 06.
Artigo em Inglês | MEDLINE | ID: mdl-28985754

RESUMO

We investigated cross-protective immunity of a US spike-insertion deletion porcine epidemic diarrhea virus (PEDV) Iowa106 (S-INDEL) strain against the original US PEDV (PC21A) strain in nursing piglets. Piglets were inoculated orally with S-INDEL, PC21A or mock. At 20-29 days post-inoculation (dpi), all pigs were challenged with the PC21A strain. The S-INDEL-inoculated pigs had lower ileal IgA antibody secreting cells, serum IgA and neutralizing antibody titers compared with PC21A-inoculated pigs. No pigs in the PC21A-group developed diarrhea, whereas 81 and 100% of pigs in the S-INDEL and mock-groups had diarrhea post challenge, respectively. S-INDEL induced partial protective immunity against the original US PEDV strain.


Assuntos
Infecções por Coronavirus/veterinária , Proteção Cruzada/imunologia , Vírus da Diarreia Epidêmica Suína/imunologia , Glicoproteína da Espícula de Coronavírus/genética , Doenças dos Suínos/prevenção & controle , Vacinas Virais/uso terapêutico , Animais , Animais Recém-Nascidos/imunologia , Anticorpos Neutralizantes/imunologia , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/prevenção & controle , Infecções por Coronavirus/virologia , Mutação INDEL/genética , Mutação INDEL/imunologia , Imunoglobulina A/imunologia , Vírus da Diarreia Epidêmica Suína/genética , Glicoproteína da Espícula de Coronavírus/imunologia , Suínos , Doenças dos Suínos/imunologia , Doenças dos Suínos/virologia , Vacinas Virais/genética , Vacinas Virais/imunologia
18.
Microb Pathog ; 112: 111-116, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28942178

RESUMO

Foot-and-mouth disease virus (FMDV) is a picornavirus that causes an economically significant disease in cattle and swine. Replication of FMDV is dependent on both viral proteins and cellular factors. Nonstructural protein 2B of FMDV plays multiple roles during viral infection and replication. We investigated the roles of 2B in virus-host interactions by constructing a cDNA library obtained from FMDV-infected swine tissues, and used a split-ubiquitin-based yeast two-hybrid system to identify host proteins that interacted with 2B. We found that 2B interacted with amino acids 208-437 in the C-terminal region of the eEF1G subunit of eukaryotic elongation factor 1, which is essential for protein synthesis. The 2B-eEF1G interaction was confirmed by co-immunoprecipitation of 2B and eEF1G in HEK293T cells. Collectively, our results suggest that eEF1G interacts with the 2B protein of FMDV. The identified 2B interaction partner may help to elucidate the mechanisms of FMDV infection and replication.


Assuntos
Vírus da Febre Aftosa/metabolismo , Fator 1 de Elongação de Peptídeos/metabolismo , Domínios e Motivos de Interação entre Proteínas , Técnicas do Sistema de Duplo-Híbrido , Proteínas não Estruturais Virais/isolamento & purificação , Animais , Modelos Animais de Doenças , Febre Aftosa , Vírus da Febre Aftosa/patogenicidade , Células HEK293 , Interações Hospedeiro-Patógeno , Humanos , Imunoprecipitação/métodos , Ligação Proteica , Suínos , Proteínas Virais , Proteínas Virais Reguladoras e Acessórias/metabolismo , Replicação Viral
19.
Appl Microbiol Biotechnol ; 101(12): 4905-4914, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28365796

RESUMO

Foot-and-mouth disease (FMD) is an acute and highly contagious disease caused by foot-and-mouth disease virus (FMDV) that can affect cloven-hoofed animal species, leading to severe economic losses worldwide. Therefore, the development of a safe and effective new vaccine to prevent and control FMD is both urgent and necessary. In this study, we developed a chimeric virus-like particle (VLP) vaccine candidate for serotype O FMDV and evaluated its protective immunity in guinea pigs. Chimeric VLPs were formed by the antigenic structural protein VP1 from serotype O and segments of the viral capsid proteins (VP2, VP3, and VP4) from serotype A. The chimeric VLPs elicited significant humoral and cellular immune responses with a higher level of anti-FMDV antibodies and cytokines than the control group. Furthermore, four of the five guinea pigs vaccinated with the chimeric VLPs were completely protected against challenge with 100 50% guinea pig infectious doses (GPID50) of the virulent FMDV strain O/MAY98. These data suggest that chimeric VLPs are potential candidates for the development of new vaccines against FMDV.


Assuntos
Proteínas do Capsídeo/genética , Vírus da Febre Aftosa/imunologia , Febre Aftosa/prevenção & controle , Vacinas de Partículas Semelhantes a Vírus/imunologia , Vacinas Virais/imunologia , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Baculoviridae/genética , Proteínas do Capsídeo/imunologia , Cobaias , Sorogrupo , Vacinas de Partículas Semelhantes a Vírus/administração & dosagem , Vacinas Virais/administração & dosagem
20.
Genome Announc ; 5(9)2017 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-28254990

RESUMO

Porcine epidemic diarrhea virus (PEDV) could cause an acute and highly contagious enteric disease in swine. Here, we report the complete genome sequence of PEDV strain CH/HNZZ47/2016 isolated from suckling piglets with mild diarrhea in Henan Province, China. It will help understand the molecular and evolutionary characteristics of PEDV in China.

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