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1.
BMC Infect Dis ; 19(1): 1017, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31791265

RESUMO

BACKGROUND: Early diagnosis and treatment of neurosyphilis is of great significance for regression. There is no gold standard for the diagnosis of neurosyphilis. We did this study to explore the factors associated with the clinical diagnosis of neurosyphilis and assess their accuracy for the diagnosis of neurosyphilis. METHODS: We retrospectively reviewed 100 cases of syphilis patients who underwent lumbar puncture at a major dermatology hospital in Guangzhou, China between April 2013 and November 2016. Fifty patients who were clinically diagnosed with neurosyphilis were selected as case group. Control group consisted of 50 general syphilis patients who were matched with age and gender. The records of patients were reviewed to collect data of socio-demographic information, clinical symptom, and laboratory indicators. Multivariable logistic regression was used to explore diagnostic indictors, and ROC analysis was used to assess diagnostic accuracy. RESULTS: Neurological symptoms (odds ratio (OR) = 59.281, 95% CI:5.215-662.910, P = 0.001), cerebrospinal fluid (CSF) Treponema pallidum particle agglutination (TPPA) titer (OR = 1.004, 95% CI:1.002-1.006, P < 0.001), CSF protein (OR = 1.005, 95% CI:1.000-1.009, P = 0.041), and CSF white blood cell (WBC) (OR = 1.120, 95% CI:1.017-1.233, P = 0.021) were found to be statistically associated with neurosyphilis. In ROC analysis, CSF TPPA titer had a sensitivity of 90%, a specificity of 84%, and an area under curve (AUC) of 0.941. CONCLUSION: CSF TPPA can potentially be considered as an alternative test for diagnosis of neurosyphilis. Combining with neurological symptoms, CSF protein, CSF WBC, the diagnosis would have a higher sensitivity.

2.
Oncol Rep ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31746401

RESUMO

Hepatocellular carcinoma (HCC) is a common disease of the digestive system with no curative treatments. Long noncoding RNA tyrosine protein kinase transmembrane receptor 1 antisense RNA 1 (lncRNA ROR1­AS1) is an lncRNA whose functions have been predicted in human diseases; however, its important role in cancer has been probed only in mantle cell lymphoma, not in HCC. Therefore, the present study aimed to elucidate the prognostic significance of lncRNA ROR1­AS1 in HCC. The Cancer Genome Atlas Liver Hepatocellular Carcinoma was used to analyze the expression of ROR1­AS1 in liver cancer. χ2 tests were performed to evaluate associations between clinical characteristics and ROR1­AS1 expression. The role of ROR1­AS1 in HCC prognosis was assessed using Kaplan­Meier curves and proportional hazards model (Cox) analysis. Gene set enrichment analysis was performed by using a Gene Expression Omnibus dataset. At the same time, Multi Experiment Matrix was used to predict genes that may be co­expressed with ROR1­AS1. The Database for Annotation, Visualization and Integrated Discovery and KO­Based Annotation System were used to analyze the most closely associated cytological behaviors and pathways in HCC. Then, the genes in the three databases were integrated to screen mRNAs, microRNAs and lncRNAs that had co­expression relationships with ROR1­AS1. Cytoscape, Search Tool for the Retrieval of Interacting Genes/Proteins and Molecular Evolutionary Genetics Analysis were used to map potential regulatory networks and developmental relationships associated with ROR1­AS1. Finally, 12 genes most closely associated with ROR1­AS1 were identified, and their relationship was described using a Circos plot. The results showed that ROR1­AS1 was upregulated in HCC, and its expression was related to clinical stage, T stage and N stage. Furthermore, Kaplan­Meier curves and Cox analysis indicated that high expression of ROR1­AS1 was associated with poor prognosis, and that ROR1­AS1 was an independent risk factor for HCC. Co­expression data suggested that there may be a large regulatory network of 45 genes with indirect associations with ROR1­AS1, a small regulatory network of 15 genes with direct or indirect regulatory relationships, and a special regulatory network containing 12 genes directly associated with ROR1­AS1. The present findings indicated that high expression of ROR1­AS1 suggests poor prognosis in patients with HCC.

3.
IUBMB Life ; 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31675148

RESUMO

microRNAs (miRNAs) can be used as biomarkers for acute myocardial infarction (AMI). However, few reports have focused on the value of exosomal miRNAs in the mechanism of the pathophysiological process from stable coronary artery disease (SCAD) to AMI. Exosomes were isolated via ultracentrifugation after serum samples were collected. The exosomes were then identified by transmission electron microscopy, western blotting, and nanoparticle tracking analysis. The differential expression of miRNAs in exosomes from six AMI and six matching SCAD patients was screened using the Agilent Human miRNA Microarray Kit. Target genes of the candidate miRNAs were predicted via an online miRNA database, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes analyses. Further validation was conducted through quantitative real-time polymerase chain reaction with 60 exosome samples. The expression of 13 miRNAs was significantly downregulated in the AMI samples compared with the SCAD samples. In addition, we identified various target genes that are mainly involved in the pathways of cardiac rehabilitation and remodelling. Validation of the expression of candidate miRNAs indicated that exosomal miR-1915-3p, miR-4,507, and miR-3,656 were significantly less expressed in AMI samples than in SCAD samples, and area under the receiver-operating-characteristic curve (AUC) analysis showed that the expression of these miRNAs resulted in good predictive accuracy [miR-1915-3p (AUC: 0.772); miR-4,507 (AUC: 0.684); and miR-3,656 (AUC: 0.771)], suggesting that these serum exosomal miRNAs might be predictive for AMI at an early stage. Hence, exosomal miRNAs might play an important role in the pathophysiology of AMI and could serve as diagnostic biomarkers.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31573392

RESUMO

Background: Appropriate surgical techniques to control hemorrhage and retain residual liver function are key to treatment success for hepatocellular carcinoma (HCC). This study aimed to evaluate the clinical application of Glissonean pedicle transection with hepatic vein exclusion (HVE). Materials and Methods: Between April 2013 and December 2015, 50 patients underwent surgical resection for HCC and were randomly allocated to receive Glissonean pedicle transection with HVE (Glisson group, n = 25) or Pringle maneuver with intermittent clamping (Pringle group, n = 25). Intraoperative blood loss, blood transfusion, operation time, positive surgical margins, complications (bile leakage, hemorrhage, and ascites), and hospital stay were compared between groups, along with the alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TB) levels at baseline and postoperative days 1, 3, and 7. Results: The operation time and range of hepatic resection were comparable between groups. Although both groups had similar preoperative ALT, AST, and TB levels, these levels on postoperative days 1, 3, and 7 were significantly lower in the Glisson group than in the Pringle group (all P < .01). Compared with the Pringle group, the Glisson group had a significantly lower intraoperative blood loss (P < .001), a lower blood transfusion rate (P = .017), lower incidence rates of postoperative hemorrhage (P = .030) and ascites (P = .024), a lower positive surgical margin rate (P = .017), and a shorter length of hospital stay (P < .001). Conclusions: Glissonean pedicle transection with HVE is a safe, simple, and effective procedure for hepatic resection.

5.
BMC Surg ; 19(1): 140, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31590641

RESUMO

BACKGROUND: Primary hepatic leiomyoma (PHL) is a rare manifestation of tumors in the liver; it is mainly characterized by its origin in the mesenchymal tissue. To date, the mechanisms underlying the pathogenesis of this disease remain unclear, however most reported PHL patients suffer from acquired immunity deficiency syndrome (AIDS) or take immunosuppressive medications after organ transplantation. CASE PRESENTATION: In this case report we describe a rare case of PHL in a middle-aged Chinese woman who was asymptomatic with no history of hepatitis or other liver disease. She had no history of immune suppression medication therapy. In view of the benign features of the hepatic lesion, along with our implementation of the respecting the patience choices, a laparoscopic partial hepatectomy of the right lower liver was performed, which appeared to be highly effective and give a good prognosis. CONCLUSIONS: Clinical characteristics of the patient should be compared to previously reported aspects of this disease to reach a clear diagnosis. Moreover, although PHL is extremely rare, it should still be considered a possibility. Surgical intervention is effective in treating this disease.

6.
Sci Rep ; 9(1): 13714, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31548577

RESUMO

Breast cancer is the most common malignant cancer in women. CYP24A1 expression regulates cellular response to vitamin D, which has antitumor effects against breast cancer. This study aimed to identify the correlation between CYP24A1 mRNA expression and prognosis of breast cancer. This study enrolled 1102 patients, including 1090 females and 12 males, from TCGA-BRCA cohort. The Cancer Genome Atlas database was used to study CYP24A1 mRNA expression in breast cancer, and Chi-squared tests were performed to test the correlation between clinical features and CYP24A1 expression. The prognostic value of CYP24A1 in breast cancer was assessed using Kaplan-Meier curves and Cox analysis. Low CYP24A1 expression was associated with age, molecular subtype, ER, PR, HER2, menopause status, N classification, vital status, overall survial and relapse-free survival. CYP24A1 presented a moderate diagnostic ability in breast cancer. Furthermore, low CYP24A1 expression was correlated with poor prognosis. CYP24A1 was an independent risk factor for breast cancer. CYP24A1 plays an important role in prognosis of breast cancer. CYP24A1 has the potential to be a biomarker, especially in predicting prognosis.

7.
Sensors (Basel) ; 19(18)2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31487810

RESUMO

Monitoring of tool wear in machining process has found its importance to predict tool life, reduce equipment downtime, and tool costs. Traditional visual methods require expert experience and human resources to obtain accurate tool wear information. With the development of charge-coupled device (CCD) image sensor and the deep learning algorithms, it has become possible to use the convolutional neural network (CNN) model to automatically identify the wear types of high-temperature alloy tools in the face milling process. In this paper, the CNN model is developed based on our image dataset. The convolutional automatic encoder (CAE) is used to pre-train the network model, and the model parameters are fine-tuned by back propagation (BP) algorithm combined with stochastic gradient descent (SGD) algorithm. The established ToolWearnet network model has the function of identifying the tool wear types. The experimental results show that the average recognition precision rate of the model can reach 96.20%. At the same time, the automatic detection algorithm of tool wear value is improved by combining the identified tool wear types. In order to verify the feasibility of the method, an experimental system is built on the machine tool. By matching the frame rate of the industrial camera and the machine tool spindle speed, the wear image information of all the inserts can be obtained in the machining gap. The automatic detection method of tool wear value is compared with the result of manual detection by high precision digital optical microscope, the mean absolute percentage error is 4.76%, which effectively verifies the effectiveness and practicality of the method.

8.
BMC Psychiatry ; 19(1): 293, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31533672

RESUMO

BACKGROUND: Schizophrenia is a severe mental disease with high morbidity and heritability. The SLC39A8 gene is located in 4q24 and encodes a protein that transports many metal ions. Multiple previous studies found that one of the most pleiotropic single nucleotide polymorphisms (SNPs) in SLC39A8, rs13107325, is associated with schizophrenia in the European population. However, the polymorphism of this locus is rare in other populations. In China, the Han Chinese and the Uygur Chinese are two ethnic populations that originate from different races. METHODS: A case-control study was conducted with 983 schizophrenia cases and 1230 healthy controls of the Chinese Uygur population. To validate the most promising SNP, meta-analyses were conducted with the Han Chinese and the European PGC2 data sets reported previously. RESULTS: A susceptible locus, rs10014145 (pallele = 0.014, pallele = 0.098 after correction; pgenotype = 0.004, pgenotype = 0.032 after correction) was identified in case-control study of the Chinese Uygur population. Further, the association between rs10014145 and schizophrenia was supported by a meta-analysis of Han and Uygur Chinese samples (pooled OR [95% CI] =1.10 [1.03-1.17], Z = 2.73, p = 0.006). The association between rs10014145 and schizophrenia was not significant in a meta-analysis of combined Chinese and European samples (pooled OR [95% CI] =1.07 [1.00-1.14], Z = 1.88, and p = 0.06). In addition, the "CCAC" haplotype of rs4698844-rs233814-rs13114343-rs151394 was significantly associated with schizophrenia in Uygur Chinese (P = 0.003, corrected p = 0.012). CONCLUSIONS: The results of this study support that SLC39A8 is a susceptible gene for schizophrenia in the populations of Han Chinese and Uygur Chinese in China, further studies are suggested to validate the association.

10.
ChemSusChem ; 12(19): 4412-4420, 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31429210

RESUMO

A mixed-surfactant-assisted method was developed to synthesize dual-phase Li4 Ti5 O12 -TiO2 hierarchical microspheres. The ratio of anionic/cationic surfactant could regulate the primary structure morphology and the dual-phase ratio of the final product, in which the primary structure morphology could be stacked nanosheets, small nanoparticles, or large nanoparticles. The sample with a primary structure morphology of small nanoparticles had the highest specific surface area of 79.38 m2 g-1 and the best electrochemical performance because of its high Li+ migration rate, low polarization, and appropriate TiO2 content. Its capacity reached 153.5 mA h g-1 at a current rate of 40 C, and it retained nearly 100 % of its capacity after 100 cycles. A self-assembly mechanism of the mixed surfactant was highlighted to explain the formation of hierarchical microspheres. The physical and electrochemical properties of obtained material were correlated effectively.

11.
Genomics ; 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31376529

RESUMO

Deltamethrin (DM) is widely used in a variety of pest control, resulting in serious drug resistance. Keap1-Nrf2-ARE is the antioxidant stress pathway. We identified 268 genes differentially expressed (DEGs) in Drosophila Kc cells treated with DM, including up-regulated 180 genes and down-regulated 88 genes compared with the control group (fold-change≥2, qValue≤0.001) by RNA-seq, they are mainly linked to metabolic process, stimulation response, immune system process. When the cells are treated with DM in the case of overexpression of the Keap1 gene, the cytochrome P450 family genes were significantly down-regulated, and some diseases-related genes and non-coding genes also changed. Our data shown that Keap1-Nrf2-ARE pathway may play an important role in DM stress, which will provide a new direction for studying the mechanism of insect resistance.

12.
Oncol Rep ; 42(3): 1110-1124, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31322274

RESUMO

Hepatocellular carcinoma (HCC) is a common digestive system disease with no curative treatment. Zinc finger protein 385D antisense RNA 2 (ZNF385D­AS2) is a long non­coding RNA (lncRNA) that has been predicted to function in human diseases, including several types of cancer. Yet, it has not been investigated in relation to liver cancer. Thus, the present study was designed with an aim to elucidate the prognostic significance of lncRNA ZNF385D­AS2 in HCC. The Cancer Genome Atlas­Liver Hepatocellular Carcinoma (TCGA­LIHC) collection of data was utilized to analyze the expression of lncRNA ZNF385D­AS2 in liver cancer. Then Chi­square tests were used to evaluate the correlation between clinical characteristics and lncRNA ZNF385D­AS2 expression. The significance of lncRNA ZNF385D­AS2 in patient prognosis was evaluated using Kaplan­Meier curves and Cox analysis. Concomitantly, Gene Set Enrichment Analysis (GSEA) was performed to analyze the most closely related cytological behavior. Finally, we used the Database for Annotation, Visualization and Integrated Discovery (DAVID) and KOBAS software and data from the Gene Expression Omnibus (GEO) database to analyze the possible competing endogenous RNA (ceRNA) network pattern as well as the co­expression network in liver cancer. Based on the results, analysis of RNA­Seq gene expression data for 303 patients with primary tumors revealed low expression of ZNF385D­AS2 in liver cancer. Low expression of ZNF385D­AS2 was found to be significantly associated with sex (P=0.050), T stage (P=0.049), M stage (P=0.040), N stage (P<0.001) and clinical stage (P=0.037). Patients with ZNF385D­AS2 low­expression liver cancers had a shorter median overall survival compared with the patients with ZNF385D­AS2 high­expression liver cancers (P=0.0079). Cox analysis identified ZNF385D­AS2 low­expression as an independent prognostic variable (AUC=0.594) for overall survival in liver cancer patients. Co­expression and ceRNA predictive analysis data suggested that there may be a regulatory signaling axis between ZNF385D­AS2 and miR­96 and miR­182. In conclusion, our results suggests that low expression of ZNF385D­AS2 is predictive of a poor prognosis of liver cancer patients.

13.
Brain Res ; 1721: 146333, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31302097

RESUMO

After associative learning, a signal induces the recall of its associated signal, or the other way around. This reciprocal retrieval of associated signals is essential for associative thinking and logical reasoning. For the cellular mechanism underlying this associative memory, we hypothesized that the formation of synapse innervations among coactivated sensory cortices and the recruitment of associative memory cells were involved in the integrative storage and reciprocal retrieval of associated signals. Our study indicated that the paired whisker and olfaction stimulations led to an odorant-induced whisker motion and a whisker-induced olfaction response, a reciprocal form of associative memory retrieval. In mice that showed the reciprocal retrieval of associated signals, their barrel and piriform cortical neurons became mutually innervated through their axon projection and new synapse formation. These piriform and barrel cortical neurons gained the ability to encode both whisker and olfaction signals based on synapse innervations from the innate input and the newly formed input. Therefore, the associated activation of sensory cortices by pairing input signals initiates their mutual synapse innervations, and the neurons innervated by new and innate synapses are recruited to be associative memory cells that encode these associated signals. Mutual synapse innervations among sensory cortices to recruit associative memory cells may compose the primary foundation for the integrative storage and reciprocal retrieval of associated signals. Our study also reveals that new synapses onto the neurons enable these neurons to encode memories to new specific signals.

14.
J Chromatogr A ; 1603: 311-326, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31272731

RESUMO

Herein, we report a multi-residue method based on dissolution-precipitation extraction combined with gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis for targeted screening and quantification of 216 harmful chemicals in plastic children's toys. We established a spectrogram database containing the first-order mass spectra of all analytes and their second-order mass spectra at different collision energies. Good separation was attained in less than 60 min. As a simple and low-cost extraction method, dissolution-precipitation exhibited good recoveries for the analytes in the plastic samples. The matrix-matched standard curves were accurate for analyte quantification in specific plastics due to the solvent effects and matrix effects. The limits of quantification range were 0.1 mg/kg to 10 mg/kg. A wide linear range of 0.1-200 mg/kg was also observed, with r2≥0.9924. The average recoveries ranged from 63.9% to 137.6%, and the relative standard deviation (n = 6) varied from 0.6% to 13.2%. Finally, 31 ABS actual toys and 30 PVC actual toys were detected and 20 analytes with contents ranging from 0.4 mg/kg to 172 mg/kg and 30 analytes from 0.8 mg/kg to 141,030 mg/kg were discovered respectively.


Assuntos
Cromatografia Gasosa-Espectrometria de Massas/métodos , Plásticos/química , Jogos e Brinquedos , Espectrometria de Massas em Tandem/métodos , Criança , Humanos , Reprodutibilidade dos Testes , Solventes/química
15.
Surg Endosc ; 2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31209606

RESUMO

BACKGROUND: Acute cholangitis (AC) is an acute inflammation of the biliary tract caused by bacterial infection, which occurs due to biliary obstruction primarily because of bile duct stones. We aimed to study the effect of laparoscopic common bile duct exploration in the treatment of complicated AC for elderly patients. METHOD: Elderly patients with complicated AC admitted to our hospital from August 2014 to August 2018 were considered. According to the patients' general conditions and the American Society of Anesthesiologists' (ASA) grade, 98 patients were divided into three groups: ASA grade II, 38 patients; ASA grade III, 33 patients; and ASA grade IV, 27 patients; all patients underwent emergency laparoscopic common bile duct exploration within 8 h of admission. The perioperative data of these patients were analyzed. RESULTS: There were no significant differences between the three groups in preoperative laboratory test results, except for albumin levels. Conversely, when compared in every group, there were some significant differences in changes between pre- and postoperative laboratory test results, except for albumin levels. There were no significant differences between the groups in terms of perioperative data (operation time, blood loss, peritoneal drainage time, postoperative time to flatus, and postoperative hospital stay). Although four patients had postoperative complications, there were no significant differences in the rate of complications between the groups. CONCLUSION: Laparoscopic common bile duct exploration is a safe, effective, and feasible method for treating complicated AC in elderly patients. It should be actively used in clinical work to rapidly relieve biliary obstruction.

16.
Psychiatr Genet ; 29(4): 120-126, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31162297

RESUMO

OBJECTIVE: Schizophrenia is one of the most severe mental disorders and its etiology is supposed to be an interaction between genes and environmental factors. Previous genome-wide association studies of schizophrenia have reported multiple susceptibility loci including rs6704641 in the SATB2 gene. Recently, this locus was further confirmed as a genome-wide significant locus for association with schizophrenia by trans-ancestry meta-analysis of Han Chinese and Caucasian samples. However, there is no report of genetic analysis in Uygur Chinese population, which is considered to have a combined genetic background between eastern Asia and Caucasian. This study is aimed to explore whether SATB2 gene is significantly associated with schizophrenia in Uygur Chinese population, thus providing additional evidence for elucidating the role of SATB2 gene in schizophrenia. PARTICIPANTS AND METHODS: In this study, we performed a case-control analysis focusing on seven tag single nucleotide polymorphisms located in SATB2 gene among 985 patients with schizophrenia and 1218 healthy controls recruited from the Xinjiang Province of China. RESULTS: We found that rs6704641 was significantly associated with schizophrenia in both allelic and genotypic distributions (Pallele = 0.008, Pgenotype = 0.028 after correction). In addition, rs16831466 is significantly associated with schizophrenia in allelic distributions (corrected Pallele = 0.041). Besides, several haplotypes of single nucleotide polymorphism are significantly associated with schizophrenia too. CONCLUSION: Our results suggest that SATB2 is also a susceptibility gene for schizophrenia in Uygur Chinese population, and subsequent functional experiments are necessary to reveal its role in the pathogenesis.

17.
J Cell Mol Med ; 23(8): 5154-5164, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31207081

RESUMO

Long non-coding RNA (lncRNA) deleted in lymphocytic leukaemia 1 (DLEU1) was reported to be involved in the occurrence and development of multiple cancers. However, the exact expression, biological function and underlying mechanism of DLEU1 in hepatocellular carcinoma (HCC) remain unclear. In this study, real-time quantitative polymerase chain reaction (qRT-PCR) in HCC tissues and cell lines revealed that DLEU1 expression was up-regulated, and the increased DLEU1 was closely associated with advanced tumour-node-metastasis stage, vascular metastasis and poor overall survival. Function experiments showed that knockdown of DLEU1 significantly inhibited HCC cell proliferation, colony formation, migration and invasion, and suppressed epithelial to mesenchymal transition (EMT) process via increasing the expression of E-cadherin and decreasing the expression of N-cadherin and Vimentin. Luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay demonstrated that DLEU1 could sponge miR-133a. Moreover, miR-133a inhibition significantly reversed the suppression effects of DLEU1 knockdown on HCC cells. Besides, we found that silenced DLEU1 significantly decreased insulin-like growth factor 1 receptor (IGF-1R) expression (a target of miR-133a) and its downstream signal PI3K/AKT pathway in HCC cells, while miR-133a inhibitor partially reversed this trend. Furthermore, DLEU1 knockdown impaired tumour growth in vivo by regulating miR-133a/IGF-1R axis. Collectively, these findings indicate that DLEU1 promoted HCC progression by sponging miR-133a to regulate IGF-1R expression. Deleted in lymphocytic leukaemia 1/miR-133a/IGF-1R axis may be a novel target for treatment of HCC.

18.
Chemosphere ; 231: 450-456, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31146137

RESUMO

The translation control tumor protein (TCTP) is a kind of conservative, common and important molecule, several functions (such as regulating cell cycle, apoptosis and calcium binding) have been reported. However, few academic researches for role of TCTP in insecticides stress were made so far. In this research, Drosophila kc cells treated with different doses of deltamethrin at different times, indicated that the expression of TCTP reached the highest level when the cells were treated with 20 ppm of deltamethrin at 24 h. The results showed that TCTP expression is associated with deltamethrin stress. To investigate the functional relationship between this gene and deltamethrin resistance, RNA interference (RNAi) and cell transfection were utilized. TCTP knockdown significantly reduced the level of resistance of RNAi-treated cells, and the overexpressions of TCTP in Drosophila kc cells conferred a degree of protection against deltamethrin. Flow cytometry data showed increased apoptosis rate of RNAi-treated cells and decreased apoptosis following cell transfection. These results represent the first evidence that TCTP plays an important role in the regulation of deltamethrin resistance. Therefore, this study could help us to elucidate the environmental toxicity of deltamethrin and new target genes associated with resistance.


Assuntos
Inseticidas/toxicidade , Nitrilos/toxicidade , Piretrinas/toxicidade , Testes de Toxicidade , Animais , Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Drosophila , Proteínas de Drosophila/genética , Resistência a Inseticidas/efeitos dos fármacos , Inseticidas/metabolismo , Neoplasias/genética , Interferência de RNA
19.
Biomed Pharmacother ; 114: 108862, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30981105

RESUMO

Small Nucleolar RNA Host Gene (SNHG16) is a novel cancer-related long noncoding RNA (lncRNA) and functions as an oncogene in a variety of cancers. Nonetheless, the expression patterns, biological function, and potential mechanisms in SNHG16 in pancreatic cancer (PC) remain rarely known. An increase in expression of SNHG16 in PC samples against adjacent normal tissues was shown here. Increased SNHG16 was linked intimately to the tumor-node-metastasis (TNM) stage, distant metastasis, tumor differentiation, and poor overall survival. Loss-of-function experiments revealed that SNHG16 knockdown suppressed the proliferation, formation of colonies, ability to migrate and invade in vitro, along with a lowered growth of the tumor in a mouse model. Mechanistically, SNHG16 might serve as a sponge competitive endogenous RNA (ceRNA) for miR-218-5p, thereby playing a role in regulating the expression of high mobility group box 1 (HMGB1) expression, a known direct miR-218-5p target in PC cells. These results provide novel insight into PC tumorigenesis and suggest that SNHG16 could serve as a likely therapeutic intervention in PC.


Assuntos
Proliferação de Células/genética , MicroRNAs/genética , Neoplasias Pancreáticas/genética , Neoplasias Pancreáticas/patologia , RNA Longo não Codificante/genética , Animais , Diferenciação Celular/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Regulação Neoplásica da Expressão Gênica/genética , Proteína HMGB1/genética , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Oncogenes/genética
20.
Sensors (Basel) ; 19(5)2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-30866576

RESUMO

License plate detection (LPD) is the first and key step in license plate recognition. State-of-the-art object-detection algorithms based on deep learning provide a promising form of LPD. However, there still exist two main challenges. First, existing methods often enclose objects with horizontal rectangles. However, horizontal rectangles are not always suitable since license plates in images are multi-oriented, reflected by rotation and perspective distortion. Second, the scale of license plates often varies, leading to the difficulty of multi-scale detection. To address the aforementioned problems, we propose a novel method of multi-oriented and scale-invariant license plate detection (MOSI-LPD) based on convolutional neural networks. Our MOSI-LPD tightly encloses the multi-oriented license plates with bounding parallelograms, regardless of the license plate scales. To obtain bounding parallelograms, we first parameterize the edge points of license plates by relative positions. Next, we design mapping functions between oriented regions and horizontal proposals. Then, we enforce the symmetry constraints in the loss function and train the model with a multi-task loss. Finally, we map region proposals to three edge points of a nearby license plate, and infer the fourth point to form bounding parallelograms. To achieve scale invariance, we first design anchor boxes based on inherent shapes of license plates. Next, we search different layers to generate region proposals with multiple scales. Finally, we up-sample the last layer and combine proposal features extracted from different layers to recognize true license plates. Experimental results have demonstrated that the proposed method outperforms existing approaches in terms of detecting license plates with different orientations and multiple scales.

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