Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 32
Filtrar
Mais filtros










Base de dados
Tipo de estudo
Intervalo de ano de publicação
1.
Aging (Albany NY) ; 12(1): 156-177, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31896739

RESUMO

A promising new strategy for cancer therapy is to target the autophagic pathway. However, comprehensive characterization of autophagy genes and their clinical relevance in cancer is still lacking. Here, we systematically characterized alterations of autophagy genes in multiple cancer lines by analyzing data from The Cancer Genome Atlas and CellMiner database. Interactions between autophagy genes and clinically actionable genes (CAGs) were identified by analyzing co-expression, protein-protein interactions (PPIs) and transcription factor (TF) data. A key subnetwork was identified that included 18 autophagy genes and 22 CAGs linked by 28 PPI pairs and 1 TF-target pair, which was EGFR targeted by RARA. Alterations in the expression of autophagy genes were associated with patient survival in multiple cancer types. RARA and EGFR were associated with worse survival in colorectal cancer patients. The regulatory role of EGFR in 5-FU resistance was validated in colon cancer cells in vivo and in vitro. EGFR contributed to 5-FU resistance in colon cancer cells through autophagy induction, and EGFR overexpression in 5-FU resistant colon cancer was regulated by RARA. The present study provides a comprehensive analysis of autophagy in different cancer cell lines and highlights the potential clinical utility of targeting autophagy genes.

2.
Food Funct ; 9(11): 6020-6028, 2018 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-30397690

RESUMO

Lactobacillus fermentum KP-3 was isolated from Korean pickle and used to ferment ginseng. The changes in the minor ginsenosides in the fermented ginseng were analyzed and the material was evaluated in high fat diet-fed mice. Total ginsenosides increased from 0.746 mg g-1 to 0.939 mg g-1 after fermentation, and the levels of minor ginsenosides (Rg2, Rg3, Rh1, Rh2, F2, and Ro) increased from 0.186 mg g-1 to 0.704 mg g-1. In an animal study, the serum TC and LDL levels in the HFD group were significantly higher than those of the control group. Compared with the HFD group, the probiotic-fermented ginseng significantly decreased the serum TC and LDL levels. In addition, the serum and liver ALT and AST levels were dramatically increased in the HFD group, but these increases were significantly inhibited by treatment with the probiotic-fermented ginseng. Furthermore, fermented ginseng reduced high fat diet-induced liver lipid accumulation. Overall, fermentation with L. fermentum KP-3 enhanced minor ginsenosides in ginseng and this probiotic-fermented ginseng ameliorated hyperlipidemia and liver injury induced by a high fat diet.


Assuntos
Ginsenosídeos/farmacologia , Hiperlipidemias/tratamento farmacológico , Lactobacillus fermentum/metabolismo , Hepatopatias/tratamento farmacológico , Panax/química , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Glicemia/metabolismo , Colesterol/sangue , Dieta Hiperlipídica , Fermentação , Microbiologia de Alimentos , Hiperlipidemias/sangue , Fígado/efeitos dos fármacos , Fígado/metabolismo , Hepatopatias/sangue , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Extratos Vegetais/farmacologia , Probióticos , Triglicerídeos/sangue
3.
Huan Jing Ke Xue ; 39(5): 2056-2066, 2018 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965505

RESUMO

Thirty-two samples were collected from eight typical areas in Lake Taihu. Three-dimensional excitation-emission matrix fluorescence spectra (EEMs) and a parallel factor analysis (PARAFAC) were applied to investigate the fluorescence properties, the sources, and environmental significance of particulate organic matter (POM) from the overlying water in Lake Taihu in summer. Differences in fluorescence characteristics between POM and DOM (dissolved organic matter), and that in POM between the grass lake and the algal lake were further examined. There are five kinds of fluorophores in Lake Taihu:tyrosine-like fluorophores (C1 and C2), humic-like fluorophores (C3 and C4), and tryptophan-like fluorophores (C5), among which significant correlations were found between C1 and C2, C3 and C4, and C5 and two humic-like fluorophores (C4, C3). By comparing with the fluorescence characteristics of DOM from earlier studies, it is found that there are differences in compositions, sources, and correlations with water quality between POM and DOM in Lake Taihu in summer. In summer, the contribution of endogenous inputs to POM is greater than that of exogenous inputs in Lake Taihu because the ranges in the fluorescence indices, FI, BIX, and HIX, are 1.78-2.35, 0.3-2.7, and 0.8-1.1, respectively. Significant correlations have been found between humic-like fluorescent components and TN, TP, Chla, COD, POC, and SS, suggesting that fluorescence analysis can be used as an important method for a semi-quantitative analysis of nutrients. The protein-like components of the algal lake mainly consist of tryptophan and tyrosine, while that of the grass lake mainly contain tyrosine and a few tryptophan. It is worth noting that, the grass lake contains more tyrosine relative to the algal lake (t test, P < 0.01). Significant positive correlations between the protein-like and the humic-like fluorescence are found in the algal lake, while it is not significant in the grass lake. Significant positive correlations are found between the fluorescent components (protein-like and humic-like) and chlorophyll-a in the algal lake, while the correlation was only found between humic-like components and chlorophyll-a in the grass lake.


Assuntos
Lagos/química , Material Particulado/análise , Qualidade da Água , China , Clorofilídios , Análise Fatorial , Espectrometria de Fluorescência
4.
Mol Cancer ; 16(1): 9, 2017 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-28086904

RESUMO

BACKGROUND: With more than 600,000 mortalities each year, colorectal cancer (CRC) is the third most commonly diagnosed type of cancer worldwide. Recently, mechanisms involving noncoding RNAs have been implicated in the development of CRC. METHODS: We examined expression levels of lncRNA CRNDE and miR-181a-5p in 64 cases of CRC tissues and cell lines by qRT-PCR. Gain-of-function and loss-of-function assays were performed to examine the effect of CRNDE and miR-181a-5p on proliferation and chemoresistance of CRC cells. Using fluorescence reporter and western blot assays, we also explored the possible mechanisms of CRNDE in CRC cells. RESULTS: In this study, we found that the expression levels of the CRNDE were upregulated in CRC clinical tissue samples. We identified microRNA miR-181a-5p as an inhibitory target of CRNDE. Both CRNDE knockdown and miR-181a-5p overexpression in CRC cell lines led to inhibited cell proliferation and reduced chemoresistance. We also determined that ß-catenin and TCF4 were inhibitory targets of miR-181a-5p, and that Wnt/ß-catenin signaling was inhibited by both CRNDE knockdown and miR-181a-5p overexpression. Significantly, we found that the repression of cell proliferation, the reduction of chemoresistance, and the inhibition of Wnt/ß-catenin signaling induced by CRNDE knockdown would require the increased expression of miR-181a-5p. CONCLUSIONS: Our study demonstrated that the lncRNA CRNDE could regulate the progression and chemoresistance of CRC via modulating the expression levels of miR-181a-5p and the activity of Wnt/ß-catenin signaling.


Assuntos
Resistencia a Medicamentos Antineoplásicos/genética , Regulação Neoplásica da Expressão Gênica , MicroRNAs/genética , RNA Longo não Codificante/genética , Via de Sinalização Wnt , Adulto , Idoso , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/genética , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Neoplasias Colorretais/genética , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Modelos Biológicos , Gradação de Tumores , Metástase Neoplásica , Estadiamento de Neoplasias , Interferência de RNA , Fator de Transcrição 4 , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Carga Tumoral , beta Catenina/genética , beta Catenina/metabolismo
5.
J Ind Microbiol Biotechnol ; 43(10): 1481-95, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27538968

RESUMO

This study focused on the differences in protein expression at various periods during limonene biotransformation by Penicillium digitatum DSM 62840. A total of 3644 protein-species were quantified by iTRAQ during limonene biotransformation (0 and 12 h). A total of 643 proteins were differentially expressed, 316 proteins were significantly up-regulated and 327 proteins were markedly down-regulated. GO, COG, and pathway enrichment analysis showed that the differentially expressed proteins possessed catalytic and binding functions and were involved in a variety of cellular and metabolic process. Furthermore, the enzymes involved in limonene transformation might be related to cytochrome P-450. This study provided a powerful platform for further exploration of biotransformation, and the identified proteins provided insight into the mechanism of limonene transformation.


Assuntos
Cicloexenos/metabolismo , Proteínas Fúngicas/metabolismo , Penicillium/metabolismo , Terpenos/metabolismo , Biotransformação , Cromatografia Líquida , Cicloexenos/química , Limoneno , Proteômica , Espectrometria de Massas em Tandem , Terpenos/química
6.
Molecules ; 21(4): 511, 2016 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-27110762

RESUMO

This study aimed to determine the influence of different emulsifiers or xanthan-emulsifier systems on the release of aroma compounds. Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds. The effects of the emulsifier systems supplemented with xanthan gum on aroma release were also studied in the same way. The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds. Compared with other aroma compounds, ethyl acetate was more likely to be conserved in the solution system, while the amount of limonene released was the highest among these seven aroma compounds. In conclusion, different emulsifiers and complexes showed different surface properties that tend to interact with different aroma molecules. The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.


Assuntos
Emulsificantes/química , Aditivos Alimentares/química , Odorantes/análise , Soja/química , Indústria de Processamento de Alimentos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Lecitinas/química , Polissacarídeos Bacterianos/química , Polissorbatos/química , Microextração em Fase Sólida/métodos , Sacarose/química , Propriedades de Superfície
7.
Expert Opin Ther Targets ; 20(9): 1127-35, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26998881

RESUMO

INTRODUCTION: Toll-like receptors (TLRs) are expressed by a wide variety of cell types including immune cells. They play a crucial role in the inflammatory and host defense response against microorganisms, and triggering TLRs can mediate the activation of innate immunity. Furthermore, research suggests that various TLRs may function differently on different tumor cells. The change in TLR activity may elicit an anti-tumor activity in hepatocellular carcinoma (HCC) cells and may serve as a novel therapeutic target for HCC therapy. AREAS COVERED: This review discusses the role of the TLR family in HCC and the underlying signaling pathway of TLRs as a form of pattern recognition receptor in mediating inflammation and HCC immunity responses. Agonists and antagonists of TLRs, which render TLRs as potential therapeutic targets, activate downstream molecules, subsequently causing HCC cell survival. The proliferation or protection against the development of HCC is also described. EXPERT OPINION: A series of studies have highlighted a crucial role of TLRs in HCC and consider TLR signaling pathways as potential therapeutic targets for HCC. However, the conclusions of these studies are in part paradoxical and controversial. Thus, it is necessary to extend further research to help determine the signaling pathways involved.


Assuntos
Carcinoma Hepatocelular/tratamento farmacológico , Neoplasias Hepáticas/tratamento farmacológico , Receptores Toll-Like/imunologia , Animais , Antineoplásicos/farmacologia , Carcinoma Hepatocelular/imunologia , Sobrevivência Celular/efeitos dos fármacos , Humanos , Imunidade Inata/imunologia , Neoplasias Hepáticas/imunologia , Terapia de Alvo Molecular , Transdução de Sinais/imunologia
8.
Artigo em Inglês | MEDLINE | ID: mdl-24438283

RESUMO

In this work, we report the complete mitochondrial genome sequence of a German shepherd. The total length of the mitogenome was 16,727 bp. It contained the typical structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a non-coding control region (D-loop region). The arrangement of these genes was the same as that found in other dogs. All the protein initial codons were ATG, except for ND2, ND3 and ND5 starting with ATA. Sequence comparison indicates that D-loop region harbors most sequence variations.


Assuntos
Cães/genética , Variação Genética , Genoma Mitocondrial/genética , Análise de Sequência de DNA , Animais , Genes de RNAr , Anotação de Sequência Molecular , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , RNA de Transferência/genética
9.
Oncotarget ; 7(5): 5429-39, 2016 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-26716411

RESUMO

OBJECTIVES: Neutrophil lymphocyte ratio (NLR) has been shown to predict prognosis of cancers in several studies. This study was designed to evaluate the impact of stratified NLR in patients who have received curative liver resection (CLR) for hepatocellular carcinoma (HCC). METHODS: A total of 1659 patients who underwent CLR for suspected HCC between 2007 and 2014 were reviewed. The preoperative NLR was categorized into quartiles based on the quantity of the study population and the distribution of NLR. Hazard ratios (HRs) and 95% confidence intervals (CIs) were significantly associated with overall survival (OS) and derived by Cox proportional hazard regression analyses. Univariate and multivariate Cox proportional hazard regression analyses were evaluated for association of all independent parameters with disease prognosis. RESULTS: Multivariable Cox proportional hazards models showed that the level of NLR (HR = 1.031, 95%CI: 1.002-1.060, P = 0.033), number of nodules (HR = 1.679, 95%CI: 1.285-2.194, P<0.001), portal vein thrombosis (HR = 4.329, 95%CI: 1.968-9.521, P<0.001), microvascular invasion (HR = 2.527, 95%CI: 1.726-3.700, P<0.001) and CTP score (HR = 1.675, 95%CI: 1.153-2.433, P = 0.007) were significant predictors of mortality. From the Kaplan-Meier analysis of overall survival (OS), each NLR quartile showed a progressively worse OS and apparent separation (log-rank P=0.008). The highest 5-year OS rate following CLR (60%) in HCC patients was observed in quartile 1. In contrast, the lowest 5-year OS rate (27%) was obtained in quartile 4. CONCLUSIONS: Stratified NLR may predict significantly improved outcomes and strengthen the predictive power for patient responses to therapeutic intervention.


Assuntos
Carcinoma Hepatocelular/patologia , Hepatectomia/mortalidade , Neoplasias Hepáticas/patologia , Linfócitos/patologia , Neutrófilos/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma Hepatocelular/mortalidade , Carcinoma Hepatocelular/cirurgia , Estudos de Coortes , Feminino , Seguimentos , Humanos , Neoplasias Hepáticas/mortalidade , Neoplasias Hepáticas/cirurgia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Fatores de Risco , Taxa de Sobrevida , Adulto Jovem
10.
Sci Rep ; 5: 9287, 2015 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-25787015

RESUMO

Autophagy is involved in the development and/or progression of many diseases, including myocardial ischemia/reperfusion (I/R). In this study, we hypothesized a protective role of basic fibroblast growth factor (bFGF) both in vivo and in vitro and demonstrated that excessive autophagy and ubiquitinated protein accumulation is involved in the myocardial I/R model. Our results showed that bFGF improved heart function recovery and increased the survival of cardiomyocytes in myocardial I/R model. The protective effect of bFGF is related to the inhibition of LC3II levels. Additionally, bFGF enhances the clearance of Ub by p62 and increases the survival of H9C2 cells. Moreover, silencing of p62 partially blocks the clearance of Ub and abolishes the anti-apoptosis effect of bFGF. An shRNA against the autophagic machinery Atg7 increased the survival of H9C2 cells co-treated with bFGF and rapamycin. bFGF activates the downstream signaling of the PI3K/Akt/mTOR pathway. These results indicate that the role of bFGF in myocardial I/R recovery is related to the inhibition of excessive autophagy and increased ubiquitinated protein clearance via the activation of PI3K/Akt/mTOR signaling. Overall, our study suggests a new direction for bFGF drug development for heart disease and identifies protein signaling pathways involved in bFGF action.


Assuntos
Fator 2 de Crescimento de Fibroblastos/metabolismo , Traumatismo por Reperfusão Miocárdica/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais , Serina-Treonina Quinases TOR/metabolismo , Proteínas Ubiquitinadas/metabolismo , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular , Modelos Animais de Doenças , Ecocardiografia , Fator 2 de Crescimento de Fibroblastos/farmacologia , Fibrose , Inativação Gênica , Humanos , Masculino , Camundongos , Traumatismo por Reperfusão Miocárdica/diagnóstico , Traumatismo por Reperfusão Miocárdica/genética , Traumatismo por Reperfusão Miocárdica/fisiopatologia , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Interferência de RNA , Ratos , Transdução de Sinais/efeitos dos fármacos
11.
J Cancer Res Clin Oncol ; 141(4): 661-9, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25326896

RESUMO

BACKGROUND AND OBJECTIVES: Enhancer of zeste 2 (EZH2), embryonic ectoderm development (EED), and suppressor of zeste 12 homolog (SUZ12), the key component of polycomb repressive complex 2, are of great importance in human cancer pathogenesis. This study was designed to investigate the clinical and prognostic significances of EZH2, EED and SUZ12 in colorectal cancer (CRC) patients. METHODS: The expression of EZH2, EED and SUZ12 mRNA was evaluated in 82 primary CRC and paired non-cancerous mucosa samples by qRT-PCR. RESULTS: We found that overall EZH2, EED and SUZ12 mRNA expression in the CRC tissues was significantly increased than in the non-cancerous tissue (p < 0.05). Increased EZH2, EED and SUZ12 mRNA expression was directly correlated with primary tumor size, regional lymph node metastases, distant metastasis and AJCC stage. Furthermore, CRC patients with higher level of EED, SUZ12 or EZH2 showed a worse disease-free survival (DFS) (p < 0.01). In multivariate analysis, the increased EZH2 expression may be a risk factor for the patients' 3-year DFS (HR 2.517; 95% CI 1.104, 5.736; p = 0.028). Furthermore, the k-means cluster analysis showed that high mRNA expression of EED, SUZ12 and EZH2 was significantly correlated with the aggressive clinical behavior and poor prognosis. CONCLUSIONS: High expression of EED, SUZ12 and EZH2 might contribute to the CRC development/progression.


Assuntos
Neoplasias Colorretais/genética , Regulação Neoplásica da Expressão Gênica , Complexo Repressor Polycomb 2/genética , Western Blotting , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/patologia , Progressão da Doença , Proteína Potenciadora do Homólogo 2 de Zeste , Humanos , Estimativa de Kaplan-Meier , Metástase Linfática , Análise Multivariada , Estadiamento de Neoplasias , Complexo Repressor Polycomb 2/metabolismo , Prognóstico , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa
12.
J Chromatogr A ; 1374: 58-65, 2014 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-25498552

RESUMO

Metal-organic nanotubes (MONTs), a novel class of hybrid materials, have attracted considerable attention because of their uniform and fixed internal diameters, impressive topological structures, and versatile applications. However, to the best of our knowledge, no studies on MONTs coating fabrication for solid-phase microextraction are yet available. The aim of this work is to investigate the feasibility of using [Cu3(µ3-O)(µ-OH)(triazolate)2]+ as a solid-phase microextraction coating material to enrich trace levels of polychlorinated biphenyls in water samples. The novel [Cu3(µ3-O)(µ-OH)(triazolate)2]+-coated fibers achieved large enhancement factors (396­1343), low limits of detection (3.9­21.7 pg L−1), and wide linearity (0.1­500 ng L−1) for detecting polychlorinated biphenyls. Relative standard deviations obtained ranged from 2.12 to 7.22%, and spiked PCBs recoveries (spiking concentrations of 1 and 5 ng L−1) in four environmental water samples ranged from 71.3 to 104%. These findings indicate that [Cu3(µ3-O)(µ-OH)(triazolate)2]+ as a solid-phase microextraction coating material is an excellent alternative for the rapid and sensitive analysis of trace levels of polychlorinated biphenyls in the environment.


Assuntos
Nanotubos/química , Compostos Organometálicos/química , Bifenilos Policlorados/análise , Microextração em Fase Sólida/métodos , Água/química , Estudos de Viabilidade , Microscopia Eletroquímica de Varredura , Nanotubos/ultraestrutura , Triazóis
13.
Biomed Res Int ; 2014: 807874, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24900988

RESUMO

Fibroblast growth factor 21 (FGF21) is an important endogenous regulator involved in the regulation of glucose and lipid metabolism. FGF21 expression is strongly induced in animal and human subjects with metabolic diseases, but little is known about the molecular mechanism. Endoplasmic reticulum (ER) stress plays an essential role in metabolic homeostasis and is observed in numerous pathological processes, including type 2 diabetes, overweight, nonalcoholic fatty liver disease (NAFLD). In this study, we investigate the correlation between the expression of FGF21 and ER stress. We demonstrated that TG-induced ER stress directly regulated the expression and secretion of FGF21 in a dose- and time-dependent manner. FGF21 is the target gene for activating transcription factor 4 (ATF4) and CCAAT enhancer binding protein homologous protein (CHOP). Suppression of CHOP impaired the transcriptional activation of FGF21 by TG-induced ER stress in CHOP-/- mouse primary hepatocytes (MPH), and overexpression of ATF4 and CHOP resulted in FGF21 promoter activation to initiate the transcriptional programme. In mRNA stability assay, we indicated that ER stress increased the half-life of mRNA of FGF21 significantly. In conclusion, FGF21 expression is regulated by ER stress via ATF- and CHOP-dependent transcriptional mechanism and posttranscriptional mechanism, respectively.


Assuntos
Fator 4 Ativador da Transcrição/genética , Estresse do Retículo Endoplasmático/genética , Retículo Endoplasmático/genética , Fatores de Crescimento de Fibroblastos/genética , Fator de Transcrição CHOP/genética , Ativação Transcricional/genética , Células 3T3 , Animais , Linhagem Celular , Meia-Vida , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Regiões Promotoras Genéticas/genética , Ligação Proteica/genética , Transdução de Sinais/genética , Transcrição Genética/genética
14.
Tumour Biol ; 35(10): 9619-25, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24964959

RESUMO

Mel-18 is a member of the polycomb group (PcG) of proteins, which are chromatin regulatory factors that play an important role in oncogenesis. This study was designed to investigate the clinical and prognostic significance of Mel-18 in colorectal cancer (CRC) patients. For this purpose, expression of Mel-18 mRNA was evaluated in 82 primary CRC and paired noncancerous mucosa samples by qRT-PCR and Western blotting. We found that overall Mel-18 mRNA expression in the CRC tissue was significantly lower than in the noncancerous mucosal tissue (p = 0.007, Wilcoxon matched-pairs signed-ranks test). Mel-18 was conversely correlated with the pathological classifications (p = 0.003 for T, p < 0.001 for N, and p = 0.015 for M classifications, respectively) and clinical AJCC stage (p < 0.001). Furthermore, CRC patients with a higher level of Mel-18 showed prolonged disease-free survivals (DFS) (p < 0.001). In multivariate analysis, the diminished Mel-18 expression may be a risk factor for the patients' 3-year DFS (HR = 1.895; 95 % CI 1.032, 3.477; p = 0.039). It was therefore concluded that the lower Mel-18 expression might contribute to the CRC development/progression.


Assuntos
Biomarcadores Tumorais/análise , Neoplasias Colorretais/metabolismo , Complexo Repressor Polycomb 1/biossíntese , Western Blotting , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/patologia , Intervalo Livre de Doença , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Complexo Repressor Polycomb 1/análise , Prognóstico , Modelos de Riscos Proporcionais , RNA Mensageiro/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa
15.
J Sep Sci ; 37(12): 1496-502, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24723552

RESUMO

We have investigated the feasibility of bamboo charcoal@iron oxide black for the headspace solid-phase microextraction of polychlorinated biphenyls in environmental water samples. Bamboo charcoal@iron oxide black was prepared and used as a solid-phase microextraction coating material, and gas chromatography with tandem mass spectrometry was used for detection. Several important factors affecting the extraction efficiency were systematically investigated and optimized. Under the optimum conditions, the experimental data exhibited wide linear range over the range 0.2-1000 ng/L and low limits of detection in the range of 4.7-22.2 pg/L. The novel coating was successfully used for the enrichment and determination of polychlorinated biphenyls in real environmental water samples. All these results indicated that bamboo charcoal@iron oxide black-based headspace solid-phase microextraction coupled to gas chromatography with tandem mass spectrometry was an excellent alternative for the sensitive analysis of polychlorinated biphenyls at ultratrace levels in the environment.


Assuntos
Bifenilos Policlorados/isolamento & purificação , Microextração em Fase Sólida/métodos , Poluentes Químicos da Água/isolamento & purificação , Bambusa/química , Carvão Vegetal/química , Compostos Férricos/química , Cromatografia Gasosa-Espectrometria de Massas , Bifenilos Policlorados/química , Sensibilidade e Especificidade , Microextração em Fase Sólida/instrumentação , Poluentes Químicos da Água/química , Poluição Química da Água/análise
16.
J Surg Oncol ; 109(3): 234-8, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24449194

RESUMO

BACKGROUND AND OBJECTIVES: To test prognostic significance of lymph node status in patients with metastatic colorectal carcinoma (mCRC). METHODS: Four hundred ninety six patients diagnosed with synchronous mCRC and treated with lymphadenectomy between 1995 and 2008 were identified and divided into groups pN0, pN1, and pN2 (140 (28.2%) in pN0, 223 (45.0%) in pN1, and 133 (26.8%) in pN2 group) according to their lymph node status. The Kaplan-Meier and Cox regression analyses were used to test associations and independent predictor status of lymph node involvement. RESULTS: The Cox proportional hazards regression showed pN as significantly associated with disease-specific survival (DSS) both in univariate (HR = 1.609, 95% CI 1.411 to 1.835, P < 0.001) and multivariate (HR = 1.630, 95% CI 1.422 to 1.868, P < 0.001) analyses. The Kaplan-Meier analysis demonstrated that patients with pN2 and pN1 had a significantly worse DSS compared with patients with pN0 tumors (respectively, 17.273 ± 1.020 and 27.145 ± 1.715 vs. 34.992 ± 2.143 months; P < 0.001). In accuracy analyses based on AUC values, nodal status demonstrated the highest accuracy (65.1%) out of all the variables. CONCLUSIONS: Our findings indicate that optimal TNM staging for mCRC should incorporate lymph node status to provide a more effective and predictive model.


Assuntos
Neoplasias Colorretais/patologia , Neoplasias Colorretais/cirurgia , Excisão de Linfonodo , Linfonodos/patologia , Linfonodos/cirurgia , Adulto , Idoso , Neoplasias Colorretais/mortalidade , Intervalo Livre de Doença , Feminino , Humanos , Estimativa de Kaplan-Meier , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Neoplasias Primárias Múltiplas/cirurgia , Valor Preditivo dos Testes , Prognóstico , Modelos de Riscos Proporcionais , Estudos Retrospectivos
17.
Nutr Cancer ; 65(7): 1034-41, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24053672

RESUMO

Genistein, a major isoflavone found in soybeans, exhibits anticarcinogenic properties. The inhibitory effect of genistein on cell proliferation is associated with G2/M cell cycle arrest and inhibition of cdc2 activities. Here we assessed the role of PTEN in regulation of genistein-mediated G2/M cell cycle arrest in the gastric cancer cell lines (SGC-7901 and BGC-823). After 24 h following treatment, genistein induced a concentration-dependent accumulation of cells in the G2/M phase of the cell cycle. The sustained G2/M arrest by genistein in SGC-7901 and BGC-823 cells is associated with increased phospho-cdc2 (Tyr15) and decreased cdc2 protein. Genistein treatment increased Wee1 levels and decreased phospho-Wee1 (Ser 642). Moreover, genistein substantially decreased the Ser473 and Thr308 phosphorylation of Akt and upregulated PTEN expression. Downregulation of PTEN by siRNA in genistein-treated cells increased phospho-Wee1 (Ser642), whereas decreased phospho-Cdc2 (Tyr15), resulting in decreased the G2/M cell cycle arrest. Therefore, induction of G2/M cell cycle arrest by genistein involved upregulation of PTEN.


Assuntos
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Genisteína/farmacologia , PTEN Fosfo-Hidrolase/metabolismo , Neoplasias Gástricas/metabolismo , Proteína Quinase CDC2 , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Divisão Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Ciclina B/genética , Ciclina B/metabolismo , Quinases Ciclina-Dependentes , Regulação para Baixo , Fase G2/efeitos dos fármacos , Humanos , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , PTEN Fosfo-Hidrolase/genética , Proteínas Tirosina Quinases/genética , Proteínas Tirosina Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Regulação para Cima
18.
Anal Chim Acta ; 769: 65-71, 2013 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-23498122

RESUMO

In this paper, bamboo charcoals were modified using Fe3O4 nanosheets for the first time. The composites, as a novel solid-phase microextraction (SPME) fiber coating, were used for the extraction of seven polybrominated diphenyl ethers (PBDEs) in environmental water samples. The extraction factors (stirring rate, extraction time, and ionic strength) and desorption factors (desorption time and desorption temperature) of the fibers were systematically investigated and optimized. Under optimum conditions, the linear range was 1-1000 ng L(-1). Based on the ratio of chromatographic signal to base line noise (SN(-1)=3), the limits of detection (LODs) can reach 0.25-0.62 ng L(-1). The novel method was successful in the analysis of PBDEs in real environmental water samples. The results indicate that bamboo charcoal/Fe3O4 as an SPME coating material coupled with gas chromatography-negative chemical ionization-mass spectrometry is an excellent method for the routine analysis of PBDEs at trace levels in environmental water samples.


Assuntos
Carvão Vegetal/química , Óxido Ferroso-Férrico/química , Cromatografia Gasosa-Espectrometria de Massas , Éteres Difenil Halogenados/análise , Poluentes Químicos da Água/análise , Adsorção , Bambusa/química , Éteres Difenil Halogenados/isolamento & purificação , Microextração em Fase Sólida , Poluentes Químicos da Água/isolamento & purificação
19.
Anal Bioanal Chem ; 405(14): 4993-6, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23529412

RESUMO

This study demonstrates the potential of bamboo charcoal as a novel and inexpensive solid-phase microextraction (SPME) coating material for enrichment and determination of organic pollutants in water samples. Bamboo charcoal was prepared and used as a SPME coating material. Eleven phthalate esters (PAEs) were used as model analytes, and gas chromatography-mass spectrometry was used for separation and detection. Important extraction conditions (ionic strength, stirring rate, and extraction time) and desorption conditions (desorption temperature and time) were systematically investigated and optimized. Linearity of 0.1-100 µg L(-1) and correlation coefficients of 0.9992-0.9998 were obtained under optimum conditions. Inter-day and intra-day repeatability were 2.15-9.93 % and 1.89-9.85 %, respectively, and fiber-to-fiber reproducibility was 5.42-9.66 %. On the basis of a chromatographic signal-to-baseline noise ratio of three, the limits of detection reached 0.004-0.023 µg L(-1). Satisfactory results were achieved when the bamboo coating was used for determination of 11 PAEs in real water samples. The experimental results indicate that bamboo charcoal has significant potential as a SPME coating material for rapid enrichment and sensitive determination of organic pollutants in environmental samples.

20.
Anal Bioanal Chem ; 404(5): 1603-10, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22766759

RESUMO

This paper demonstrates, for the first time, the great potential of using Zn/Al layered double hydroxide intercalated sodium dodecyl benzene sulfonate (Zn/Al-SDBS-LDH) as a solid-phase extraction (SPE) material in the extraction of persistent organic pollutants prior to the determination of gas chromatography-mass spectrometry in environmental water samples. Zn/Al-SDBS-LDH, a relatively inexpensive and simply prepared material, was synthesized and used as a SPE adsorbent to quantitatively determine the concentration of five polycyclic aromatic hydrocarbons (PAHs) in environmental water samples. Factors affecting extraction efficiency, such as, eluent type, eluent volume, flow rate of sample, sample volume, and amount of adsorbent, were investigated and optimized in detail. Experimental results indicate that there is an excellent linear relationship between peak area and the concentration of PAHs over the range of 5-500 ng L(-1), and the precisions (relative standard deviation (RSD)) were 2.5-6.3% under the optimum conditions. Based on the ratio of chromatographic signal-to-base line noise (S/N = 3), the limits of detection could reach 1.2-3.2 ng L(-1). This novel method was successfully applied to the analysis of PAHs in environmental water samples. As such, we show here that the use of Zn/Al-SDBS-LDH as SPE adsorbent materials, coupled with gas chromatography-mass spectrometry, is an excellent improvement in the routine analysis of PAHs at trace levels in the environment.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA