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1.
Bioact Mater ; 21: 20-31, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36017068

RESUMO

Hydrogel patch-based stem cell transplantation and microenvironment-regulating drug delivery strategy is promising for the treatment of myocardial infarction (MI). However, the low retention of cells and drugs limits their therapeutic efficacies. Here, we propose a prefixed sponge carpet strategy, that is, aldehyde-dextran sponge (ODS) loading anti-oxidative/autophagy-regulating molecular capsules of 2-hydroxy-ß-cyclodextrin@resveratrol (HP-ß-CD@Res) is first bonded to the rat's heart via capillary removal of interfacial water from the tissue surface, and the subsequent Schiff base reaction between the aldehyde groups on ODS and amino groups on myocardium tissue. Then, an aqueous biocompatible hydrazided hyaluronic acid (HHA) solution encapsulating mesenchymal stem cells (MSCs) is impregnated into the anchored carpet to form HHA@ODS@HP-ß-CD@Res hydrogel in situ via click reaction, thus prolonging the in vivo retention time of therapeutic drug and cells. Importantly, the HHA added to outer surface consumes the remaining aldehydes to contribute to nonsticky top surface, avoiding adhesion to other tissues. The embedded HP-ß-CD@Res molecular capsules with antioxidant and autophagy regulation bioactivities can considerably improve cardiac microenvironment, reduce cardiomyocyte apoptosis, and enhance the survival of transplanted MSCs, thereby promoting cardiac repair by facilitating angiogenesis and reducing cardiac fibrosis.

2.
Food Chem ; 399: 133799, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35998490

RESUMO

Flesh quality is evaluated according to nutritional value and sensory quality. Cinnamaldehyde (CIN) improves mammalian meat quality, but research relating this to aquaculture is scarce. In this study, five doses of CIN (0, 36, 72, 108, 144 mg/kg diet) were fed to grass carp (Ctenopharyngodon idella) for 60 days. The results show that CIN supplementation increased nutritional value by increasing crude protein content. CIN also improved the sensory quality by increasing the pH and collagen content, decreasing shear force, lactate, and cooking loss. These changes may be related to changes in muscle fiber growth by increasing myofiber diameter. The increased myofiber diameter induced by CIN is associated with TOR mRNA and protein levels, and down-regulated FOXO3a mRNA levels, which might be associated with PTP1B/IGF1/PI3K/AKTs-TOR/FOXO3a signaling. Based on muscle crude protein content, optimal CIN supplementation dosage was 88.01 mg/kg.


Assuntos
Carpas , Doenças dos Peixes , Acroleína/análogos & derivados , Ração Animal/análise , Animais , Carpas/genética , Carpas/metabolismo , Dieta , Suplementos Nutricionais , Doenças dos Peixes/genética , Proteínas de Peixes/metabolismo , Imunidade Inata , Mamíferos/genética , Fibras Musculares Esqueléticas/metabolismo , Proteínas Musculares/metabolismo , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , RNA Mensageiro/genética , Transdução de Sinais
3.
Sci Total Environ ; 851(Pt 2): 158200, 2022 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-36049690

RESUMO

Humic acid has the advantages of wide source, easy availability and environmental friendliness, which may be a good choice for inhibiting chalcopyrite biooxidation and alleviating copper pollution. However, there are few researches on the inhibitory effect and mechanism of humic acid on the biooxidation of chalcopyrite. In order to fill this knowledge gap, this study proposed and validated a novel method for inhibiting chalcopyrite biooxidation by means of humic acid. The results showed that the biooxidation of chalcopyrite could be effectively inhibited by humic acid, which consequently decreased the release of copper ions. Humic acid with a concentration of 120 ppm had the best inhibitory effect, which reduced the biooxidation efficiency of chalcopyrite from 40.7 ± 0.5 % to 29.3 ± 0.8 %. This in turn suggested that humic acid could effectively suppress the pollution of copper under these conditions. The analysis results of solution parameters, mineral surface morphology, mineral phases and element composition showed that humic acid inhibited the growth of Acidithiobacillus ferrooxidans, promoted the formation of jarosite and intensified the passivation of chalcopyrite, which effectively hindered the biooxidation of chalcopyrite, and would help to alleviate the pollution of copper.

4.
Artigo em Inglês | MEDLINE | ID: mdl-36070273

RESUMO

The performance of zero-shot learning (ZSL) can be improved progressively by learning better features and generating pseudosamples for unseen classes. Existing ZSL works typically learn feature extractors and generators independently, which may shift the unseen samples away from their real distribution and suffers from the domain bias problem. In this article, to tackle this challenge, we propose a variational autoencoder (VAE)-based framework, that is, joint Attentive Region Embedding with Enhanced Semantics (AREES), which is tailored to advance the zero-shot recognition. Specifically, AREES is end-to-end trainable and consists of three network branches: 1) attentive region embedding is used to learn the semantic-guided visual features by the attention mechanism (AM); 2) a decomposition structure and a semantic pivot regularization are used to extract enhanced semantics; and 3) a multimodal VAE (mVAE) with the cross-reconstruction loss and the distribution alignment loss is used to obtain a shared latent embedding space of visual features and semantics. Finally, features' extraction and features' generation are optimized together in AREES to address the domain shift problem to a large extent. The comprehensive evaluations on six benchmarks, including the ImageNet, demonstrate the superiority of the proposed model over its state-of-the-art counterparts.

5.
IEEE Trans Cybern ; PP2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36070279

RESUMO

In this article, the optimal control strategy for organism is investigated by using the adaptive dynamic programming (ADP) method under the architecture of nonzero-sum games (NZSGs). First, a tumor model is established to formulate the interaction relationships among normal cells, tumor cells, endothelial cells, and the concentrations of drugs. Then, the ADP-based method of single-critic network architecture is proposed to approximate the coupled Hamilton-Jacobi equations (HJEs) under the medicine dosage regulation mechanism (MDRM). According to the game theory, the approximate MDRM-based optimal strategy can be derived, which is of great practical significance. Owing to the proposed mechanism, the dosages of the chemotherapy and anti-angiogenic drugs can be regulated timely and necessarily. Furthermore, the stability of the closed-loop system with the obtained strategy is analyzed via the Lyapunov theory. Finally, a simulation experiment is conducted to verify the effectiveness of the proposed method.

6.
Ann Bot ; 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36075207

RESUMO

BACKGROUND AND AIMS: With some 7,300 extant species, liverworts (Marchantiophyta) represent one of the major land plant lineages. The backbone relationships, such as the phylogenetic position of Ptilidiales, and the occurrence and timing of whole genome duplications, are still contentious. METHODS: Based on analyses of the newly generated transcriptome data for 38 liverworts and complemented with those publicly available, we reconstructed the evolutionary history of liverworts and inferred gene duplication events along the 55-taxon liverwort species tree. KEY RESULTS: Our phylogenomic study provided an ordinal-level liverwort nuclear phylogeny and identified extensive gene tree conflicts and cyto-nuclear incongruences. Gene duplication analyses based on integrated phylogenomics and Ks distributions indicated no evidence of whole genome duplication events along the backbone phylogeny of liverworts. CONCLUSIONS: With a broadened sampling of liverwort transcriptomes, we re-evaluated the backbone phylogeny of liverworts, and provided evidence for ancient hybridizations followed by incomplete lineage sorting that shaped the deep evolutionary history of liverworts. The lack of whole genome duplication along the deep evolution of liverworts indicates that liverworts might represent one of the few major embryophyte lineages whose evolution was not driven by whole genome duplications.

7.
Nat Mater ; 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36075967

RESUMO

Rare earth (RE) addition to steels to produce RE steels has been widely applied when aiming to improve steel properties. However, RE steels have exhibited extremely variable mechanical performances, which has become a bottleneck in the past few decades for their production, utilization and related study. Here in this work, we discovered that the property variation of RE steels stems from the presence of oxygen-based inclusions. We proposed a dual low-oxygen technology, and keeping low levels of oxygen content in steel melts and particularly in the raw RE materials, which have long been ignored, to achieve impressively stable and favourable RE effects. The fatigue life is greatly improved by only parts-per-million-level RE addition, with a 40-fold improvement for the tension-compression fatigue life and a 40% enhancement of the rolling contact fatigue life. We find that RE appears to act by lowering the carbon diffusion rate and by retarding ferrite nucleation at the austenite grain boundaries. Our study reveals that only under very low-oxygen conditions can RE perform a vital role in purifying, modifying and micro-alloying steels, to improve the performance of RE steels.

8.
Nat Commun ; 13(1): 5235, 2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36068270

RESUMO

Coronavirus disease 2019 (COVID-19), primarily a respiratory disease caused by infection with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is often accompanied by gastrointestinal symptoms. However, little is known about the relation between the human microbiome and COVID-19, largely due to the fact that most previous studies fail to provide high taxonomic resolution to identify microbes that likely interact with SARS-CoV-2 infection. Here we used whole-metagenome shotgun sequencing data together with assembly and binning strategies to reconstruct metagenome-assembled genomes (MAGs) from 514 COVID-19 related nasopharyngeal and fecal samples in six independent cohorts. We reconstructed a total of 11,584 medium-and high-quality microbial MAGs and obtained 5403 non-redundant MAGs (nrMAGs) with strain-level resolution. We found that there is a significant reduction of strain richness for many species in the gut microbiome of COVID-19 patients. The gut microbiome signatures can accurately distinguish COVID-19 cases from healthy controls and predict the progression of COVID-19. Moreover, we identified a set of nrMAGs with a putative causal role in the clinical manifestations of COVID-19 and revealed their functional pathways that potentially interact with SARS-CoV-2 infection. Finally, we demonstrated that the main findings of our study can be largely validated in three independent cohorts. The presented results highlight the importance of incorporating the human gut microbiome in our understanding of SARS-CoV-2 infection and disease progression.


Assuntos
COVID-19 , Microbioma Gastrointestinal , Microbiota , Microbioma Gastrointestinal/genética , Humanos , Metagenoma/genética , SARS-CoV-2/genética
9.
J Environ Public Health ; 2022: 7506894, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36072498

RESUMO

In order to improve the security of the storage and scheduling of health privacy data inside and outside the university physical education class, a storage and scheduling method based on blockchain hybrid encryption is proposed. The distribution structure model of health privacy data blockchain inside and outside the university physical education class is established, arithmetic coding and quantitative feature analysis methods to schedule and adaptively control health privacy data blockchain inside and outside the university physical education class are adopted, public key coding configuration and vector quantization coding methods are combined to design encryption keys in the process of health privacy data transmission inside and outside the university physical education class, and blockchain hybrid encryption algorithm is adopted to design encryption keys for health privacy data inside and outside the university physical education class. The arithmetic coding is embedded in the encryption system, and the bit sequence output by the blockchain hybrid encryption is circularly shifted, so as to realize the encryption of health privacy data inside and outside the university physical education class and optimize the storage scheduling. The simulation results show that this method has good security encryption performance, strong antiattack ability, and balanced storage space allocation, which improves the security storage and transmission ability of health privacy data inside and outside the university physical education class.


Assuntos
Blockchain , Segurança Computacional , Humanos , Educação Física e Treinamento , Privacidade , Universidades
10.
Comput Intell Neurosci ; 2022: 4620738, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36059408

RESUMO

In subject teaching, subject characteristics are the logical starting point for teaching development, and a deep understanding of subject characteristics is the basis for effective teaching. In practice, due to ignoring the cross-cultural understanding of middle school English subjects, there has been a long-term dilemma of "emphasizing knowledge and neglecting culture," and overemphasis on the instrumental nature of middle school English has caused middle school English teaching to lose cultural self-cultivation and obscured the due education human value. Under the background of globalization and diversification, it is more and more important to develop students' cross-cultural awareness. How to realize cross-culturalism in middle school English teaching has become an important subject of teaching reform and development. From the perspective of English subject, this research constructs a scientific and implementable English listening and speaking teaching mode that promotes students' in-depth learning, provides specific and operational teaching implementation plans for English subject teachers in front-line teaching, and improves classroom teaching. It can promote students' construction of the meaning of language knowledge, stimulate their learning motivation, and finally achieve the common development of teachers and students.


Assuntos
Aprendizado Profundo , Idioma , Comunicação , Comparação Transcultural , Humanos , Estudantes
11.
Front Public Health ; 10: 837238, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36062111

RESUMO

With the rapid development of digital information technology, life has become more convenient for people; however, the digital divide for the elderly was even more serious, so they became a forgotten group in the internet age over time. Residents' demand for healthcare is rising, but the wisdom healthcare service supported by digital information technology is less acceptable to the elderly due to the digital divide. Based on the knowledge gap theory and combining the value perception and satisfaction model, this study explores the influence of the digital divide for the elderly on wisdom healthcare satisfaction and takes the perceived value of wisdom healthcare as a mediator, and artificial intelligence and big data as moderators into the research framework. Based on the data of 1,052 elderly people in China, the results show that the digital divide for the elderly has a negative influence on wisdom healthcare satisfaction and perceived value. Moreover, it is found that wisdom healthcare perception value mediated the relationship between the digital divide for the elderly and the wisdom healthcare satisfaction, which enhances the negative effect of the digital divide for the elderly on wisdom healthcare satisfaction. Furthermore, the moderating effect of artificial intelligence and big data on the relationship between the digital divide for the elderly and the perceived value of wisdom healthcare is opposite to that between the perceived value of wisdom healthcare and wisdom healthcare satisfaction. Therefore, this study has a reference value for the development and optimization of smart medical industry.


Assuntos
Big Data , Exclusão Digital , Idoso , Inteligência Artificial , Atenção à Saúde , Humanos , Indústrias
12.
J Exp Med ; 219(11)2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36066492

RESUMO

Cell surfaces display a wide array of molecules that confer identity. While flow cytometry and cluster of differentiation (CD) markers have revolutionized cell characterization and purification, functionally heterogeneous cellular subtypes remain unresolvable by the CD marker system alone. Using hematopoietic lineages as a paradigm, we leverage the extraordinary molecular diversity of heparan sulfate (HS) glycans to establish cellular "glycotypes" by utilizing a panel of anti-HS single-chain variable fragment antibodies (scFvs). Prospective sorting with anti-HS scFvs identifies functionally distinct glycotypes within heterogeneous pools of mouse and human hematopoietic progenitor cells and enables further stratification of immunophenotypically pure megakaryocyte-erythrocyte progenitors. This stratification correlates with expression of a heptad of HS-related genes that is reflective of the HS epitope recognized by specific anti-HS scFvs. While we show that HS glycotyping provides an orthogonal set of tools for resolution of hematopoietic lineages, we anticipate broad utility of this approach in defining and isolating novel, viable cell types across diverse tissues and species.


Assuntos
Hematopoese , Anticorpos de Cadeia Única , Citometria de Fluxo , Hematopoese/genética , Células-Tronco Hematopoéticas , Heparitina Sulfato , Humanos , Estudos Prospectivos
13.
Plant Commun ; : 100429, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36071667

RESUMO

Arbuscular mycorrhizal symbiosis (AMS) is an ancient plant-fungus interaction that is widely distributed in terrestrial plants. The formation of symbiotic devices and bidirectional nutritional exchange requires the participation of numerous genes. However, the landscape of RNAome during plant AMS involving different types of regulatory RNA is poorly understood. In this study, a strategy combining multiple sequencing approaches was used to decipher the landscape of RNAome in tomato, an emerging model AMS host, during AMS. The tomato genome annotation was improved by a multiple-platform sequencing strategy. A total of 3174 protein-coding genes were upregulated during AMS, including 42 percent of them that underwent alternative splicing. Comparative-transcriptome analysis revealed that genes from 24 orthogroups were consistently induced by AMS in eight phylogenetically distant angiosperms. Seven additional orthogroups were specifically induced by AMS in all surveyed dicot AMS host plants, but were absent or not induced in monocots and/or AMS non-hosts, suggesting a continuous evolving AMS-responsive network in addition to a conserved core regulatory module. Moreover, we detected 587 lncRNAs, 10 miRNAs, and 146 circRNAs that responded to AMS, which were incorporated to establish a tomato AMS-responsive competing endogenous RNA (ceRNA) network. Finally, A tomato symbiotic transcriptome database (TSTD, https://efg.nju.edu.cn/TSTD) was constructed to serve as a resource for deep deciphering AMS regulatory network. Our results help elucidating tomato RNAome reconfiguration during AMS and suggest a sophisticated and evolving RNA layer responsive network during AMS processes.

15.
Comput Intell Neurosci ; 2022: 9037010, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36045993

RESUMO

Deep learning has an increasingly far-reaching impact on classroom teaching and is an important trend driving the application of educational technology in schools. In the traditional lecture-style teaching process, students are mostly in a passive listening and memorizing state. Simple memorization and repeated training have a certain hindering effect on promoting the transfer and application of the learner's knowledge, and it is not suitable to exert the learner's subjectivity. This research uses the current rapidly developing wireless communication technology to transmit data to the English teaching management platform, so as to realize the information exchange between the English classroom monitoring terminal and the English teaching management platform. The monitoring terminal of the system is mainly responsible for data collection of information, such as student campus card information, the location of the monitoring terminal, and terminal equipment parameters, and uses wireless communication information technology to upload it to the English teaching management platform according to the communication protocol agreed between the terminal and the platform, and the English teaching management platform can issue instructions to the monitoring terminal, so as to realize the control and management functions of the monitoring terminal. The design of the English teaching management platform of this system is a Web system designed based on B/S architecture. The interface of the system is diverse, and English teachers can easily view the monitoring data on the platform through this system. The main function of the English teaching management platform is to receive the data collected from the monitoring terminal, parse the information, and display the processing results to the English teacher in the form of a page. In order to facilitate the storage of data, MySQL database is used for the background data storage of the English teaching management platform. Through the design of the hardware terminal module and the development of the English teaching management platform, a deep learning-based English smart classroom management prototype system is realized.


Assuntos
Aprendizado Profundo , Humanos , Ensino
16.
Zhongguo Zhong Yao Za Zhi ; 47(15): 4128-4135, 2022 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-36046903

RESUMO

This study aims to investigate the effect of modified Danggui Shaoyao Powder on the suppressor of cytokine signaling 3(SOCS3)/Toll-like receptor 4(TLR4) signaling pathway in gastric tissue of rats with chronic atrophic gastritis(CAG).Sixty SPF-grade SD rats were randomly assigned into the normal group, model group, Moluo Pills group, and high-, medium-, and low-dose groups of modified Danggui Shaoyao Powder.The rats in other groups except the normal group were treated with N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) to establish the CAG model.After 12 weeks of modeling, the rats in each group were administrated with corresponding drugs by gavage for 8 weeks.After the last administration, the histopathological changes of rat gastric mucosa were observed via hematoxylin-eosin(HE) staining.The serum levels of IL-6, TNF-α, and CRP were determined by enzyme-linked immunosorbent assay(ELISA).The mRNA levels of SOCS3 and TLR4 were determined by real-time PCR.The protein levels of SOCS3, TLR4, JAK2, p-JAK2, STAT3, and p-STAT3 in rat gastric tissue were measured by Western blot.Immunohistochemical method was employed to determine the protein levels of NF-κB, MyD88, NLRP3, Bcl-2, Bax, and Bad in rat gastric tissue.The results showed that modified Danggui Shaoyao Powder alleviated gastric mucosal atrophy of rats, significantly lowered the levels of IL-6, TNF-α, and CRP in rat serum, up-regulated the mRNA level of SOCS3, and down-regulated the mRNA level of TLR4 in rat gastric tissue.Furthermore, modified Danggui Shaoyao Powder up-regulated the protein level of SOCS3, down-regulated the protein levels of TLR4, p-JAK2, p-STAT3, NF-κB, MyD88, NLRP3, Bax, and Bad, and promoted the expression of Bcl-2 protein.Therefore, modified Danggui Shaoyao Powder may mitigate the gastric mucosal atrophy of rats by regulating the SOCS3/TLR4 signaling pathway.


Assuntos
Gastrite Atrófica , Receptor 4 Toll-Like , Animais , Atrofia , Gastrite Atrófica/tratamento farmacológico , Gastrite Atrófica/genética , Interleucina-6/metabolismo , Fator 88 de Diferenciação Mieloide/genética , Fator 88 de Diferenciação Mieloide/metabolismo , NF-kappa B/genética , NF-kappa B/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Pós , RNA Mensageiro , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Proteína 3 Supressora da Sinalização de Citocinas/genética , Proteína 3 Supressora da Sinalização de Citocinas/metabolismo , Receptor 4 Toll-Like/genética , Receptor 4 Toll-Like/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Proteína X Associada a bcl-2/metabolismo
17.
Front Nutr ; 9: 976400, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36051900

RESUMO

Acrolein (ACR), an α, ß-unsaturated aldehyde, is a toxic compound formed during food processing, and the use of phenolics derived from dietary materials to scavenge ACR is a hot spot. In this study, rutin, a polyphenol widely present in various dietary materials, was used to investigate its capacity to scavenge ACR. It was shown that more than 98% of ACR was eliminated under the conditions of reaction time of 2 h, temperature of 80 °C, and molar ratio of rutin/ACR of 2/1. Further structural characterization of the formed adduct revealed that the adduct of rutin to ACR to form a cyclic hemiacetal compound (RAC) was the main scavenging mechanism. Besides, the stability of RAC during simulated in vitro digestion was evaluated, which showed that more than 83.61% of RAC was remained. Furthermore, the cytotoxicity of RAC against Caco-2 and GES-1 cells was significantly reduced compared with ACR, where the IC50 values of ACR were both below 20 µM while that of RAC were both above 140 µM. And the improvement of the loss of mitochondrial membrane potential (MMP) by RAC might be one of the detoxification pathways. The present study indicated that rutin was one of the potential ACR scavengers among natural polyphenols.

18.
Front Oncol ; 12: 917541, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36052237

RESUMO

Background: Indocyanine green (ICG) fluorescence imaging has been a new surgical navigation technique for gastric cancer. However, its clinical value should still be evaluated further. In this meta-analysis, we investigated the safety and efficacy of ICG near-infrared fluorescent imaging-guided lymph nodes (LNs) dissection during radical gastrectomy. Methods: Studies comparing ICG fluorescence imaging with standard care in patients with gastric cancer were systematically searched from PubMed, Embase, Web of Science, and Cochrane Library through August 2021. The current meta-analysis was performed according to the preferred reporting items for systematic review and meta-analysis guidelines. A pooled analysis was performed for the available data regarding the number of LNs dissection, the number of metastatic LNs dissection, other operative outcomes, and postoperative complications. R software version 4.2.0 and Stata 16.0 software were used for the present meta-analysis. Results: This analysis included 12 studies with a total of 1365 gastric cancer patients (569 in the ICG group and 796 in the non-ICG group). The number of retrieved LNs in the ICG group was significantly higher (weighted mean difference [WMD]=7.67, 95% confidence intervals [CI]: 4.73 to 10.62, P<0.05) compared to the non-ICG group with moderate heterogeneity (P<0.001, I2 = 70%). The number of metastatic LNs, operative time, and postoperative complications were all comparable and without significant heterogeneity. Additionally, ICG near-infrared fluorescent imaging was associated with reduced intraoperative blood loss (WMD=-10.28, 95% CI: -15.22 to -5.35, P<0.05) with low heterogeneity (P=0.07, I2 = 43%). Conclusions: ICG near-infrared fluorescent imaging-guided lymphadenectomy was considered to be safe and effective in gastrectomy. ICG was used to increase the number of LNs harvested while reducing intraoperative blood loss without increasing operative time or postoperative complications. Systematic Review Registration: https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42021291863.

19.
Head Neck ; 2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-36054554

RESUMO

BACKGROUND: To evaluate the predicting factors associated with distant metastasis (DM) for lymphoepithelial carcinoma of salivary gland (LECSG) following postoperative radiotherapy (PORT). METHODS: We retrospectively collected 160 eligible patients from two cancer institutions. The DM rate was evaluated using competing risk method. RESULTS: The median follow-up time was 65.6 months. Elevated preradiotherapy serum LDH (ratio >0.5) (p = 0.006) and N classification (N2-3) (p = 0.001) were independently associated with DM for the LECSG. After the risk stratification, the high-risk subgroup was defined as the patients presented higher risk score (score >0), whereas 5-year cumulative incidence of DM in the high- and low-risk group was 30.9% and 6.0%, respectively (p < 0.001). Moreover, a significantly worse overall survival (OS) was observed in the high-risk patients compared with the low-risk subgroup (5-year OS: 83.9% vs. 97.8%, p = 0.006). CONCLUSION: Elevated preradiotherapy serum LDH could serve as a predictive factor for DM in the LECSG following PORT.

20.
J Biol Chem ; : 102443, 2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36055408

RESUMO

Spinal cord injury (SCI) is the most severe result of spine injury, but no effective therapy exists to treat SCI. We have previously shown that the E3 ubiquitin ligase Two RING fingers and DRIL 1 (Triad1) promotes neurite outgrowth after SCI. However, the mechanism by which Triad1 affects neuron growth and the potential involvement of its ubiquitination activity is unclear. Neuroprotective cytokine pleiotrophin (PTN) can promote microglia proliferation and neurotrophic factor secretion to achieve neuroprotection. We found using immunostaining and behavioral assays in rats that the expression of Triad1 and the PTN was peaked at 1 day after SCI, and that Triad1 improved motor function and histomorphological injury. We also show using flow cytometry and astrocyte/neuronal co-culture assays that Triad1 overexpression promoted PTN protein levels, neurotrophic growth factor (NGF) expression, brain-derived neurotrophic factor (BDNF) expression, astrocyte and neuronal viability, and neurite outgrowth, but suppressed astrocyte apoptosis, while shRNA-mediated knockdown of Triad1 and PTN had the opposite effects. Ubiquitin ligase murine double mutant 2 (MDM2) has previously been demonstrated to participate in the process of neurite outgrowth, and mediate ubiquitination of p53. Furthermore, we demonstrate overexpression of MDM2 downregulated PTN protein levels, NGF expression, and BDNF expression in astrocytes, and inhibited neurite outgrowth of neurons. In addition, MDM2 facilitated PTN ubiquitination, which was reversed by Triad1. Finally, we show simultaneous sh-PTN and MDM2 overexpression attenuated the neurite outgrowth-promoting effect of Triad1 overexpression. In conclusion, we propose Triad1 promotes astrocyte-dependent neurite outgrowth to accelerate recovery after SCI by inhibiting MDM2-mediated PTN ubiquitination.

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