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1.
Anat Rec (Hoboken) ; 2020 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-32396707

RESUMO

AIMS: The purpose of this study is to explore the anti-tumor properties of Resibufogenin (RB) in non-small cell lung cancer (NSCLC) and elucidate its underlying mechanism. METHODS: A549 and H520 cells were treated with various concentrations of RB with or without NLRP3 inhibitor (MCC950), caspase-1 inhibitor (VX765) or N-acetyl-L-cysteine (NAC, a ROS scavenger). Cell counting Kit-8 (CCK-8) and colony formation assays were conducted to determine cell viability. Cell invasion was detected by using Transwell assay. The release of lactate dehydrogenase (LDH) was determined by LDH detection assay. The protein expression levels of related-genes were measured by western blotting and immunohistochemistry. The reactive oxygen species (ROS) level was detected by using 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA) ROS assay kit. The in vivo effects of RB were evaluated in a xenograft mouse model. RESULTS: RB treatment reduced cell viability and invasion in a dose-dependent manner. Furthermore, RB also enhanced pyroptosis levels in A549 and H520 cells, as indicated by the increased release of LDH and pyroptosis-related proteins. Interestingly, we also found that the anti-proliferative and anti-metastatic effects of RB were alleviated by the blockade of pyroptosis using NLRP3 inhibitor MCC950. Further study demonstrated that RB induced pyroptosis in a caspase-1-dependent manner, as evidenced by the finding that VX765 effectively reversed the effects of RB on A549 and H520 cells. We also found that RB could trigger caspase-1-dependent pyroptosis through ROS-mediated NF-κB suppression. CONCLUSION: In summary, our findings provide a potential antitumor agent, and a novel insight into the mechanism of RB treatment of NSCLC. This article is protected by copyright. All rights reserved.

2.
Carbohydr Polym ; 232: 115766, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31952583

RESUMO

Type 2 diabetes mellitus plagues many people in China and the world, and its nephritis complication is the leading cause of death for patients. Paecilomyces hepiali contained various functional components, especially polysaccharides, which possesses well pharmacological activities. In this study, polysaccharide purified from Paecilomyces hepiali fermented mycelium entitled PHEA was obtained, and its structure was systemically characterized using fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR). In C57BL/KsJ (BKS).Cg-Dock7m +/+ Leprdb/JNju mice (db/db mice), via detecting the alternations on biochemical criterions, pathological indicators and protein expressions related to nuclear factor-E2-related factor 2 (Nrf2) and nuclear factor-κB (NF-κB) signaling in serum and/or tissues including muscle, liver and kidney after 8-week PHEA administration, the hypoglycemic, hypolipidemic, and anti-diabetic nephropathic activities of PHEA were confirmed. The purified P. Hepiali polysaccharide with the anti-diabetic and -nephritic properties was first reported in this study via regulating Nrf2-meadited NF-κB signaling in db/db mice.

3.
Oxid Med Cell Longev ; 2020: 4054520, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31998436

RESUMO

Aronia melanocarpa (AM), which is rich in anthocyanins and procyanidins, has been reported to exert antioxidative and anti-inflammatory effects. This study aimed to systematically analyze the components of AM and explore its effects on alcohol-induced chronic liver injury in mice. A component analysis of AM revealed 17 types of fatty acids, 17 types of amino acids, 8 types of minerals, and 3 types of nucleotides. Chronic alcohol-induced liver injury was established in mice via gradient alcohol feeding over a period of 6 months, with test groups orally receiving AM in the last 6 weeks. AM administration yielded potential hepatoprotective effects by alleviating weight gain and changes in organ indexes, decreasing the ratio of alanine aminotransferase/aspartate aminotransferase, reducing lipid peroxidation, enhancing antioxidant activities, decreasing oxidation-related factor levels, and regulating inflammatory cytokine levels. Histological analyses suggest that AM treatment markedly prevented organ damage in alcohol-exposed mice. Furthermore, AM activated nuclear factor erythroid 2-like 2 (Nrf2) by downregulating the expression of Kelch-like ECH-associated protein 1, resulting in elevated downstream antioxidative enzyme levels. AM activated Nrf2 via modulation of the phosphatidylinositol-3-hydroxykinase/protein kinase B signaling pathway. Altogether, AM prevented alcohol-induced liver injury, potentially by suppressing oxidative stress via the Nrf2 signaling pathway.

4.
Opt Express ; 27(20): 28214-28222, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31684578

RESUMO

Real-time detection of the ultrafast dynamics in complex nonlinear optical systems provides novel insights into pulse interactions and dynamic patterns, especially for soliton molecules. Herein, the concept of soliton molecule is extended to the pulsating regime, revealing the dynamical diversity of soliton molecule and the universality of pulsating behavior. By virtue of the dispersive Fourier transform (DFT) technique, we present the first experimental observation of the dissociation dynamics within a pulsating soliton molecule generated in an L-band normal-dispersion mode-locked fiber laser. The results provide valuable references for resolving the interactions in complex dissipative systems.

5.
Opt Express ; 27(13): 17729-17742, 2019 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-31252729

RESUMO

As one of the most striking localized structures in dissipative systems, pulsating soliton has been widely studied in theory but rarely observed in experiments. Here, three typical types of soliton pulsations are experimentally demonstrated in an L-band normal-dispersion mode-locked fiber laser via the dispersive Fourier transform (DFT) technique. According to the distinctive features, they are classified as single-periodic pulsating soliton, double-periodic pulsating soliton and soliton explosion. These pulsations have common features such as energy oscillation, bandwidth breathing and temporal shift. However, the pulse is repeated every two oscillations for double-periodic pulsating soliton. When it comes to soliton explosion, because of the intermittent overdriven nonlinear effect induced by the extreme energy oscillation, the spectrum cracks into pieces at a periodic manner. To the best of our knowledge, it is the first time that both pure soliton pulsations and soliton explosion are observed experimentally in the same fiber laser. The results will enhance a more comprehensive understanding for the soliton pulsating phenomena.

6.
Oxid Med Cell Longev ; 2019: 1354345, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30881587

RESUMO

This study investigated the hypoglycemic and renal protective effects of scutellarin (SCU) in db/db mice and elucidated the underlying mechanisms. The oral administration of metformin hydrochloride (Met) at 120 mg/kg and SCU at 25, 50, and 100 mg/kg over an eight-week period had hypoglycemic effects, demonstrated by decreases in body weight, blood glucose, food and water intake, and glycated hemoglobin activity and by augmented insulin levels and pyruvate kinase activity in the serum of db/db mice. SCU alleviated dyslipidemia by decreasing the levels of triglycerides and total cholesterol and enhancing the levels of high-density lipoprotein cholesterol in the serum of db/db mice. SCU reversed the overexpression of mRNA of renal damage markers (receptor for advanced glycation end products, neutrophil gelatinase-associated lipocalin, and kidney injury molecule 1), macrophage marker CD11b, and T cell marker CD3 in kidney of db/db mice. Pathological examination confirmed that SCU improved the organ structures of hyperglycemia-damaged livers, kidneys, and pancreas islets. Antibody array assay and enzyme-linked immunosorbent assay were combined to screen and analyze the regulatory effects of SCU on inflammatory factors and oxidative enzymes. SCU exerted anti-inflammatory effects by inhibiting the levels of proinflammatory cytokines (glycogen synthase kinase, intercellular adhesion molecule 2, and interleukin 1ß and 2) and promoting anti-inflammatory cytokines (interleukin 4). SCU decreased the reactive oxygen species and malondialdehyde concentrations and increased the activity levels of antioxidative enzymes (superoxide dismutase, glutathione peroxidase, and catalase) in serum and kidneys. Furthermore, SCU upregulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), which in turn improved heme oxygenase 1 (HO-1), superoxide dismutase 1 and 2, and catalase expression levels in kidneys. The study showed that SCU has at least partial hypoglycemic and renal protective effects in db/db mice, and the mechanism is the modulation of the Nrf2/HO-1 signaling pathway.


Assuntos
Apigenina/uso terapêutico , Glucuronatos/uso terapêutico , Heme Oxigenase-1/efeitos dos fármacos , Rim/efeitos dos fármacos , Metformina/uso terapêutico , Fator 2 Relacionado a NF-E2/efeitos dos fármacos , Animais , Apigenina/farmacologia , Glucuronatos/farmacologia , Heme Oxigenase-1/metabolismo , Hipoglicemiantes , Metformina/farmacologia , Camundongos , Fator 2 Relacionado a NF-E2/metabolismo , Transdução de Sinais
7.
Expert Opin Drug Deliv ; 15(9): 893-903, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30169978

RESUMO

INTRODUCTION: Liposomes have been extensively investigated as drug delivery vehicles. Immunoliposomes (ILs) are antibody-conjugated liposomes designed to selectively target antigen-expressing cells. ILs can be used to deliver drugs to tumor cells for improving efficacy and reducing toxicity. In addition, ILs can be used in immunoassays, immunotherapy, and imaging. Although there has been extensive coverage on ILs in the literature, only a limited number of clinical trials have been reported and no IL drug has been approved by the FDA. AREAS COVERED: Factors to consider in developing ILs are discussed, including the choice of antibody or antibody fragment, the formulation of liposomes, and the conjugation chemistry. In addition, challenges and opportunities in clinical development of ILs are discussed. The purpose of this review is to provide an overview on the state of the art of ILs and to discuss potential future developments. EXPERT OPINION: IL research has had a lengthy history and numerous preclinical studies have yielded encouraging results. However, there are a number of obstacles to clinical translation of ILs. Given the unique capabilities of ILs, its potential for clinical application is underexplored. There is great potential for expanded role for ILs in the clinic and further efforts to this end are warranted. ABBREVIATIONS: Ab: antibody; ADCs: antibody-drug conjugates; API: active pharmaceutical ingredient; ADCC: antibody-dependent cellular cytotoxicity; CR: complete remission; cGMP: current good manufacturing practice; DSPE: distearoyl phosphatidylethanolamine; EGF: epidermal growth factor; EGFR: epidermal growth factor receptor; EPR: enhanced permeability and retention; Fc: fragment crystalline; Tf: transferrin; HACA: human-anti-chimeric antibody; HAHA: human-anti-human antibody; HAMA: human-anti-mouse antibody; HER2: human epidermal growth factor 2; IL: immunoliposome; LNPs: lipid nanoparticles; MRI: magnetic resonance imaging; MTD: maximum tolerated dose; PEG: polyethylene glycol; PET: positron emission tomography; PR: partial response; PSMA: prostate-specific membrane antigen; scFv: single-chain variable fragment; SPECT: single photon emission computed tomography; TTR: transthyretin.


Assuntos
Sistemas de Liberação de Medicamentos , Imunoconjugados/administração & dosagem , Neoplasias/terapia , Humanos , Fragmentos de Imunoglobulinas , Lipídeos/química , Lipossomos , Nanopartículas/química
8.
Opt Lett ; 43(9): 2146-2149, 2018 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-29714775

RESUMO

We demonstrate that using a single cylindrical vector (CV) beam in two-mode fibers, the orbital angular momentum of light can be switched among -1, 0, and 1. The input CV beam can be a conventional radial and azimuthal polarization distribution or a generalized CV beam, and we first use and verify that a rocking-long period fiber grating generates the tunable generalized CV beam. Because of using a single CV beam as the light source, this approach not only provides an increased stability compared to the conventional superposed eigenmodes method, but also builds a bridge between the polarization singularity beams and the phase singularity beams.

9.
Oncol Lett ; 15(5): 6291-6296, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29731847

RESUMO

Acute promyelocytic leukemia is frequently associated with dizziness, fever, nausea, hematochezia and anemia. Blue light, or light with wavelengths of 400-480 nm, transmits high levels of energy. The aim of the present study was to determine the pro-apoptotic effects of blue light (wavelength, 456 nm; radiation power, 0.25 mW/cm2) and the underlying mechanisms in a human promyelocytic leukemia cell line (HL60). Blue light reduced the viability and enhanced the mortality of HL60 cells in a time-dependent manner. Exposure to blue light for 24 h caused depolarization of the mitochondrial membrane potential and the overproduction of reactive oxygen species in HL60 cells. In a nude mouse model, 9-day exposure to blue light markedly suppressed the growth of HL60-xenografted tumors; however, it had no effect on hepatic and renal tissues. In addition, blue light abrogated the expression of B-cell lymphoma (Bcl)-2 and Bcl extra-long, while enhancing the levels of Bcl-2-associated X protein, cytochrome c, and cleaved caspases-3 and -9 in tumor tissues. The results suggested that the pro-apoptotic effects of blue light in human promyelocytic leukemia cells may be associated with the mitochondrial apoptosis signaling pathway.

10.
Int J Biol Macromol ; 116: 8-15, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29730008

RESUMO

The objective of this study was to determine the ameliorative effects of Antrodia cinnamomea polysaccharide (ACPS) against cyclophosphamide (CTX)-induced immunosuppression in BALB/c mice. Four weeks of oral ACPS treatment successfully improved bodyweight and organ indexes and enhanced the function of T cells and the cytotoxicity of natural killer cells. CTX administration has been shown to notably decrease immunoglobulin A, G and M, interleukin 2, 6 and 12, and interferon α and γ levels in serum and in the spleen, and ACPS abolished these effects. Furthermore, ACPS effectively increased the total antioxidant capacity by stimulating superoxidase dismutase, catalase, and glutathione peroxidase activity in serum and in the spleen and by inhibiting the increases in reactive oxygen species and malondialdehyde levels. Notably, ACPS induced the activation of erythroid 2-related factor 2 (Nrf2) related to down-regulating Kelch-like ECH-associated protein 1 expression, which leads to enhanced levels of downstream antioxidative enzymes, including heme oxygenase-1 (HO-1), superoxide dismutase 2, and catalase in the spleen and thymus. Therefore, the protective effects of ACPS on CTX-induced immunosuppression in mice may be the result of a reduction in oxidative stress and involved in the Nrf2/HO-1 pathway. Our study suggests that ACPS has potential for development as an effective anti-immunosuppressive agent.


Assuntos
Ciclofosfamida/farmacologia , Heme Oxigenase-1/metabolismo , Imunossupressores/farmacologia , Proteínas de Membrana/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Polissacarídeos/farmacologia , Animais , Antioxidantes/metabolismo , Antrodia , Catalase/metabolismo , Glutationa Peroxidase/metabolismo , Imunossupressão/métodos , Proteína 1 Associada a ECH Semelhante a Kelch/metabolismo , Malondialdeído/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/efeitos dos fármacos , Baço/efeitos dos fármacos , Baço/metabolismo , Superóxido Dismutase/metabolismo
11.
Opt Lett ; 43(8): 1662-1665, 2018 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-29652334

RESUMO

After a fiber Bragg grating (FBG) is fabricated, the reflection spectrum of the FBG is generally not tunable without mechanical deformation or temperature adjustment. Here we present a microcavity-coupled FBG with both a tunable reflection lineshape and dispersion using electromagnetically induced transparency. The Fano interference of light in the FBG and the microcavity allows for dramatic modification of the reflection spectrum. The phase of the reflected spectrum is continuously tunable between 0 and 2π to produce various Fano lineshapes. The dispersion of the output light is adjustable from normal dispersion to abnormal dispersion, consequently providing an adjustable speed of light. Additionally, it allows the FBG to switch from a notch filter to a bandpass filter at the resonant wavelength, which is not possible in a conventional uniform FBG.

12.
Opt Express ; 26(6): 7013-7021, 2018 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-29609386

RESUMO

A long-period fiber grating (LPFG) mode converter based on a two-mode polarization-maintaining photonic crystal fiber (PM-PCF) is proposed and demonstrated. The mode converters realize conversions between the LP01 modes and LP11a modes with parallel polarization directions. Different from typical conventional mode converters, the PM-PCF-LPFG mode converters most notably can separate out two linearly polarized LP11a modes at different wavelengths. The highest mode-conversion efficiency is more than 99%. In addition, the bandwidth of the mode converter is adjustable by changing the grating number of the LPFG.

13.
Opt Lett ; 43(3): 478-481, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-29400819

RESUMO

We experimentally observe soliton self-organization and pulsation in a passively mode-locked fiber laser. The optomechanical interaction in the optical fiber is key to the formation of equidistant soliton bunches. These solitons simultaneously undergo a pulsation process with a period corresponding to tens of the cavity round trip time. Using the dispersive Fourier transformation technique, we find that the Kelly sidebands in the shot-to-shot spectra appear periodically, synchronizing with the pulsation.

14.
Opt Express ; 26(25): 33116-33128, 2018 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-30645468

RESUMO

We numerically investigate the dynamic evolution of pulsating solitons based on complex cubic-quintic Ginzburg-Landau equation with gain dynamics effects. We show that an additional soliton can be generated by the disturbance caused by a dispersion wave emitted by a single-period pulsating soliton and these solitons form soliton molecule. More complicated oscillating processes, such as snaking pulsation and double-periodic pulsation are actuated by periodic collision of the entangled solitons. Moreover, the dispersive wave, caused by high gain parameters and the soliton collision, appears periodically which is in sync with the pulsating process. These results are consistent with the recent experiments of soliton pulsations measured by dispersive Fourier transform techniques, and will stimulate further experimental research of the complex multi-soliton bunches in dissipative systems.

15.
Biomed Res Int ; 2017: 5852076, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28929115

RESUMO

This study was performed to investigate the therapeutic effects and possible mechanisms of sunflower (Helianthus annuus) head extract (SHE) on gout. First, the components of sunflower head powder and SHE were analyzed systematically. SHE, especially SHEB (extracted with 20% ethanol and 80% double-distilled water), strongly suppressed the swelling of the ankles in rats with acute gout induced by monosodium urate (MSU) crystals and reduced the levels of uric acid and xanthine oxidase (XO) in mice with hyperuricemia induced by oteracil potassium and yeast extract powder. Hematoxylin and eosin staining indicated that SHEB reduced inflammation cells and increased the joint space in the ankle compared with the control rats with MSU-induced gout. In the rats with acute gout, among 13 detected inflammatory cytokines, SHEB significantly enhanced the serum levels of interleukin-10 and the monocyte chemoattractant protein 1α. In the mice with hyperuricemia, SHEB reduced the levels of glutathione peroxidase, superoxide dismutase, malondialdehyde, and nitrogen monoxide in liver tissues. The potential therapeutic effects of SHE on gout are probably due to the production of anti-inflammatory cytokines and the suppression of XO activity via the modulation of oxidative stress status.


Assuntos
Artrite Gotosa/tratamento farmacológico , Helianthus/química , Hiperuricemia/tratamento farmacológico , Extratos Vegetais/farmacologia , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Artrite Gotosa/induzido quimicamente , Modelos Animais de Doenças , Etanol , Flores/química , Humanos , Hiperuricemia/induzido quimicamente , Inflamação , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Estresse Oxidativo , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Ratos , Ratos Sprague-Dawley
16.
Mol Med Rep ; 16(4): 5675-5682, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28849191

RESUMO

Paecilomyces hepiali contains identical chemical constituents to Cordyceps sinensis, and it presents antidepressant­like activity via regulating noradrenergic and dopaminergic systems. Behavioral despair depression models serve important roles in scientific screening and evaluation of antidepressants. The present study aims to investigate the antidepressant­like activity of P. hepiali extract (PHC) in chronic unpredictable mild stress (CUMS)­induced rat model of depression. Following four weeks of treatment, similar to fluoxetine at 3 mg/kg (positive drug), PHC at doses from 0.08 to 2.0 g/kg strongly increased sucrose preference and reduced the immobility time of depression­like rats in forced swimming test. The hypo­level of adrenocorticotropic hormone, noradrenaline and glucocorticoid receptor in serum and hypothalamus of depression­like rats was enhanced by PHC. PHC normalized CUMS­induced disorders of dihydroxyphenylacetic acid, dopamine, 5­hydroxytryptamine (5­HT) and 5­hydroxyindoleacetic acid in serum and/or hypothalamus of depression­like rats. Moreover, PHC enhanced the expression of tyrosine hydroxylase and reduced the levels of dopamine D2 receptor and 5­HT2A receptor in hypothalamus. These results suggested that the antidepressant­like effects of PHC in CUMS­induced depression are associated with not only the modulation of dopamine, but also the regulation of 5-HT.


Assuntos
Transtorno Depressivo/etiologia , Transtorno Depressivo/metabolismo , Dopamina/metabolismo , Micoses/complicações , Paecilomyces/fisiologia , Serotonina/metabolismo , Estresse Psicológico , Animais , Comportamento Animal , Doença Crônica , Modelos Animais de Doenças , Hormônios/sangue , Hormônios/metabolismo , Masculino , Micoses/microbiologia , Neurotransmissores/sangue , Neurotransmissores/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais
17.
PLoS One ; 12(6): e0180476, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28662169

RESUMO

This study evaluated the effects of Inonotus obliquus polysaccharides (IOs) on diabetes and other underlying mechanisms related to inflammatory factors and oxidative stress in a mouse model of streptozotocin (STZ)-induced diabetes. Four weeks administration of metformin (120 mg/kg) and IO1-4 (50%-80% alcohol precipitation), or IO5 (total 80% alcohol precipitation) at doses of 50 mg/kg reverses the abnormal changes of bodyweights and fasting blood glucose levels of diabetic mice. IOs significantly increased the insulin and pyruvate kinase levels in serum, and improved the synthesis of glycogen, especially for IO5. IOs restored the disturbed serum levels of superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde. The down-regulation of interleukin-2 receptor, matrix metalloproteinase-9, and the enhancement of interleukin-2 in serum of diabetic mice were significantly attenuated by IOs. Histologic and morphology examinations showed that IOs repaired the damage on kidney tissues, inhibited inflammatory infiltrate and extracellular matrix deposit injuries in diabetic mice. Compared with untreated diabetic mice, IOs decreased the expression of phosphor-NF-κB in the kidneys. These results show that IOs treatment attenuated diabetic and renal injure in STZ-induced diabetic mice, possibly through the modulation of oxidative stress and inflammatory factors. These results provide valuable evidences to support the use of I. obliquus as a hypoglycemic functional food and/or medicine.


Assuntos
Basidiomycota/química , Diabetes Mellitus Experimental/tratamento farmacológico , Hipoglicemiantes/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Polissacarídeos/farmacologia , Animais , Hemoglobina A Glicada/metabolismo , Hipoglicemiantes/uso terapêutico , Insulina/sangue , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Polissacarídeos/uso terapêutico , Piruvato Quinase/sangue , Estreptozocina
18.
Biomed Res Int ; 2017: 9374026, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28424791

RESUMO

Antrodia cinnamomea, a folk medicinal mushroom, has numerous biological effects. In this study, we aim to assess whether the antifatigue effects of A. cinnamomea mycelia (AC) and its underlying mechanisms are related to oxidative stress signaling using behavioral mouse models and biochemical indices detection. Mice were orally treated with AC at doses of 0.1, 0.3, and 0.9 g/kg for three weeks. AC had no effect on the spontaneous activities of mice indicating its safety on central nervous system. Furthermore, results obtained from weight-loaded forced swimming test, rotary rod test, and exhausted running test confirmed that AC significantly enhanced exercise tolerance of mice. Biochemical indices levels showed that these effects were closely correlated with inhibiting the depletion of glycogen and adenosine triphosphate stores, regulating oxidative stress-related parameters (superoxide dismutase, glutathione peroxidase, reactive oxygen species, and malondialdehyde) in serum, skeletal muscle, and liver of mice. Moreover, the effects of AC may be related with its regulation on the activations of AMP-activated protein kinase, protein kinase B, and mammalian target of rapamycin in liver and skeletal muscle of mice. Altogether, our data suggest that the antifatigue properties of AC may be one such modulation mechanism via oxidative stress-related signaling in mice.


Assuntos
Antrodia/química , Fadiga/tratamento farmacológico , Fadiga/patologia , Micélio/química , Estresse Oxidativo , Transdução de Sinais , Proteínas Quinases Ativadas por AMP/metabolismo , Trifosfato de Adenosina/sangue , Animais , Modelos Animais de Doenças , Feminino , Glicogênio/metabolismo , Fígado/metabolismo , Testes de Função Hepática , Masculino , Camundongos , Músculo Esquelético/metabolismo , Oxirredução , Condicionamento Físico Animal , Proteínas Proto-Oncogênicas c-akt/metabolismo , Serina-Treonina Quinases TOR/metabolismo
19.
Oxid Med Cell Longev ; 2017: 7841823, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28337253

RESUMO

In the present study, the components of A. cinnamomea (AC) mycelia were systematically analyzed. Subsequently, its hepatoprotective effects and the underlying mechanisms were explored using a mouse model of acute alcohol-induced liver injury. AC contained 25 types of fatty acid, 16 types of amino acid, 3 types of nucleotide, and 8 types of mineral. The hepatoprotective effects were observed after 2 weeks of AC treatment at doses of 75 mg/kg, 225 mg/kg, and 675 mg/kg in the mouse model. These effects were indicated by the changes in the levels of aspartate aminotransferase, alanine aminotransferase, several oxidation-related factors, and inflammatory cytokines in serum and/or liver samples. AC reduced the incidence rate of necrosis, inflammatory infiltration, fatty droplets formation, and cell apoptosis in liver detecting via histological and TUNEL assay. In addition, AC reduced the expression of cleaved caspase-3, -8, and -9 and the levels of phosphor-protein kinase B (Akt) and phosphor-nuclear factor-κB (NF-κB) in the liver samples. Collectively, AC-mediated hepatoprotective effects in a mouse model of acute alcohol-induced liver injury are the result of reduction in oxidative stress. This may be associated with Akt/NF-κB signaling. These results provide valuable evidence to support the use of A. cinnamomea as a functional food and/or medicine.


Assuntos
Antrodia/química , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Substâncias Protetoras/farmacologia , Transdução de Sinais/efeitos dos fármacos , Alanina Transaminase/sangue , Álcoois/toxicidade , Animais , Antioxidantes/metabolismo , Antrodia/metabolismo , Aspartato Aminotransferases/sangue , Caspase 3/metabolismo , Caspase 8/metabolismo , Caspase 9/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/patologia , Citocinas/sangue , Citocinas/metabolismo , Modelos Animais de Doenças , Fígado/metabolismo , Fígado/patologia , Camundongos , NF-kappa B/metabolismo , Extratos Vegetais/química , Substâncias Protetoras/química , Proteínas Proto-Oncogênicas c-akt/metabolismo , Espécies Reativas de Oxigênio/metabolismo
20.
Opt Express ; 25(2): 994-1004, 2017 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-28157993

RESUMO

We present the design and theoretical demonstration of a microstructured optical fiber (MOF) for multichannel sensing applications based on the Fano resonance among the different whispering-gallery modes (WGMs) propagating in the MOF. The proposed MOF consists of a number of capillary channels with different diameters inside a tubular frame. When the phases of the WGMs in the capillary channels and the frame are matched, the Fano resonance will occur and the resonant peaks can be observed in the output spectrum of the tubular frame resonator. Sensing signals from the individual channels can be detected by measuring the central wavelengths of the corresponding Fano resonant peaks. To demonstrate the practicality, we study a dual-channel MOF for bio-sensing applications, i.e., detecting the refractive index variation in biological samples. In the analysis, we have shown that channel 1 and 2 achieve a sensitivity of 29.0557 nm/RIU (refractive index unit) and 22.9160 nm/RIU in the TE mode; and 16.0694 nm/RIU and 13.3181 nm/RIU in the TM mode respectively, when the refractive index of the biological samples varies between 1.330 and 1.345. The new MOF can be a compact, flexible, and low-cost solution for a variety of applications including multichannel bio/chemical sensing, multi-microcavity laser, and tunable photonics devices.

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