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1.
Front Cardiovasc Med ; 9: 827860, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35369329

RESUMO

Background: The thoracoscopic trans-mitral approach can not only facilitate exposure of the ventricular septum, mitral valve, and subvalvular apparatus, it also enables the surgeons to perform concomitant mitral valve intervention. This study aimed to determine the safety and efficacy of thoracoscopic trans-mitral septal myectomy in elderly patients with hypertrophic obstructive cardiomyopathy (HOCM). Methods: We reviewed the demographic to clinical characteristics and outcomes of patients who underwent thoracoscopic trans-mitral septal myectomy in our center between April 2019 and April 2021. The population was divided into a younger group (<60 years) and an elderly group (≥60 years). Results: There were 46 and 20 patients in the younger and elderly groups, respectively. The majority of patients in the elderly group were female (39.1 vs. 80.0%, P < 0.01). Patients in the elderly group were more likely to be in New York Heart Association Class IV (2.2 vs. 80.0%, P < 0.01). The European System for Cardiac Operation Risk Evaluation II predicted mortality rates were significantly higher (3.97 ± 1.81 vs. 1.62 ± 0.86%, P < 0.01) in the elderly group. In the elderly group, a patient converted to median sternotomy due to left ventricular posterior free wall rupture following septal myectomy and mitral bioprosthetic valve replacement. The patient then underwent double-patch sandwich repair for rupture and mitral mechanical valve replacement and was eventually discharged. All patients in the elderly group were discharged, while one in the younger group died. No patient in the elderly group required permanent pacemaker implantation vs. one in the younger group. Patients in the elderly group were more likely to spend more time in the intensive care unit than those in the younger group (5.44 ± 5.80 days vs. 3.07 ± 2.72, P < 0.05). However, there was no significant intergroup difference in in-hospital mortality or complications. Importantly, the left ventricular outflow tract pressure gradient was significantly decreased from 96.15 ± 32.89 mmHg to 8.2 ± 3.42 mmHg with no residual obstruction in the elderly group. The interventricular septal thickness was significantly decreased from 19.73 ± 3.14 mm to 11.30 ± 2.23 mm. Postoperative mitral regurgitation severity was significantly improved in the elderly group. Conclusion: This study demonstrated that thoracoscopic trans-mitral septal myectomy is a feasible option for selected elderly patients with satisfactory outcomes similar to those of young patients.

2.
Metabolism ; : 155351, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36427672

RESUMO

OBJECTIVE: As the only E2 conjugating enzyme for the SUMO system, Ubc9-mediated SUMOylation has been recognized to regulate diverse biological processes, but its impact on adipocytes relevant to obesity and insulin resistance is yet to be elucidated. METHODS: We established adipocyte-specific Ubc9 deficient mice to explore the effects of Ubc9 on obesity and metabolic disorders induced by high-fat diet (HFD) in adult mice. The molecular targets of SUMOylation were explored by liquid chromatography-mass spectrometry, and the regulatory mechanism of SUMOylation in T2D was analyzed. RESULTS: Adipocyte-specific depletion of Ubc9 (AdipoQ-Cre-Ubc9fl/fl, Ubc9AKO) protected mice from HFD-induced obesity, insulin resistance, and hepatosteatosis. The Ubc9AKO mice were featured by the reduced HFD-induced endoplasmic reticulum (ER) stress and inflammatory response. Mechanically, over nutrition rendered adipocytes to undergo a SUMOylation turnover characterized by the change of SUMOylation levels and substrates. ERp44 displayed the highest change in terms of SUMOylation levels of substrates involved in ER-related functions. The lack of ERp44 SUMOylation at lysine 76 (K76) located within the thioredoxin (TRX)-like domain by Ubc9 deficiency enhanced its degradation and suppressed its covalent binding to Ero1α, an oxidase that exists in the ER but lacks ER retention motif, thereby alleviating endoplasmic reticulum stress by promoting Ero1α secretion. CONCLUSIONS: Our data suggest that modulation of ERp44 SUMOylation in adipocytes could be a feasible strategy against obesity and insulin resistance in clinical settings.

3.
Chem Sci ; 13(37): 11197-11204, 2022 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-36320480

RESUMO

Cancer cells utilize a range of migration modes to navigate through a confined tissue microenvironment in vivo, while regulatory roles of key microRNAs (miRNAs) remain unclear. Precisely engineered microconfinement and the high spatial-resolution imaging strategy offer a promising avenue for deciphering the molecular mechanisms that drive cell migration. Here, enzyme-free signal-amplification nanoprobes as an effective tool are developed for three-dimensional (3D) high-resolution profiling of key miRNA molecules in single migrating cells, where distinct migration modes are precisely driven by microconfinement-engineered microchips. The constructed nanoprobes exhibit intuitive and ultrasensitive miRNA characterization in vitro by virtue of a single-molecule imaging microscope, and the differential expression and intracellular locations in different cell lines are successfully monitored. Furthermore, 3D spatial distribution of miR-141 at high resolution in flexible phenotypes of migrating cells is reconstructed in the engineered biomimetic microenvironment. The results indicate that miR-141 may be involved in the metastatic transition from a slow to a fast migration state. This work offers a new opportunity for investigating regulatory mechanisms of intracellular key biomolecules during cell migration in biomimetic microenvironments, which may advance in-depth understanding of cancer metastasis in vivo.

4.
Front Immunol ; 13: 1061939, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36341329

RESUMO

[This corrects the article DOI: 10.3389/fimmu.2022.850998.].

5.
Chemosphere ; 309(Pt 2): 136666, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36220431

RESUMO

With the development of medical drugs, the widely used tetracycline has brought many adverse effects on the ecosystem and human health. Tetracycline pollution of water environment is becoming more and more serious, and has become an emerging environmental problem. As single celled organisms, microalgae are not only model organisms for risk assessment of aquatic ecosystems, but also can efficiently purify sewage. Microalgae-mediated pollutant remediation has attracted more and more attention from researchers. In this paper, Chlamydomonas reinhardtii (C. reinhardtii) was used to remove tetracycline in aqueous solution, and the removal efficiency and mechanism of microalgae on tetracycline were studied. The results showed that the removal rates of tetracycline by active and inactivated microalgae at a density of 5 × 106 cells·mL-1 were 81.9% and 89.8%, respectively. C. reinhardtii removed tetracycline through biosorption and nonmetabolic processes. Microalgal cell supernatant and hydroxyl radicals could significantly promote the removal of tetracycline. The positively charged tetracycline was electrostatically adsorbed on the microalgae surface and extracellular polymeric substances. Microalgae biomass can promote the production of ROS and enhance the ability of microalgae to remove tetracycline.


Assuntos
Chlamydomonas reinhardtii , Poluentes Ambientais , Microalgas , Humanos , Ecossistema , Esgotos , Espécies Reativas de Oxigênio/metabolismo , Tetraciclina/farmacologia , Microalgas/metabolismo , Biomassa , Antibacterianos/farmacologia , Água/metabolismo , Poluentes Ambientais/metabolismo , Águas Residuárias
6.
Opt Express ; 30(11): 18772-18780, 2022 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-36221671

RESUMO

Chromatic aberration is a main obstacle for the commercial application of augmented reality displays. The current digital and optical compensation methods of reducing the chromatic aberration suffer from processing time, power consumption or complex design. Here, a simple strategy of chromatic aberration correction in bi-focal augmented reality near-eye display based on multi-layer Pancharatnam-Berry phase lens has been demonstrated and verified by experimental results. The multi-layer Pancharatnam-Berry phase lens, as a part of optical combiner, is fabricated by three liquid crystal polymer phase lenses with central wavelength in red, green, and blue, respectively. The multi-layer Pancharatnam-Berry phase lens can effectively reduce the chromatic aberration in both convex and concave mode of bi-focal augmented reality system, where the color breakup of virtual images captured in bi-focal augmented reality display is significantly alleviated. Comparing to the value of ΔK = 1.3 m-1 in single green Pancharatnam-Berry phase lens, the multi-layer Pancharatnam-Berry phase lens system significantly reduce the ΔK to 0.45 m-1 with reduction of 65.4%, which finally decreases the longitudinal chromatic aberration and improve the quality of images. The proposed broadband multi-layer Pancharatnam-Berry phase lens can benefit augmented reality displays and find widespread application in the near-eye displays.

7.
Int J Mol Sci ; 23(19)2022 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-36232451

RESUMO

Growing evidence suggests that gut microbiota are associated with atherosclerosis (AS). However, the functional heterogeneity of each gut segment gives rise to regional differences in gut microbiota. We established a rat model of AS by feeding the rats a high-fat diet for a long period. The pathological and microbiota changes in the ileum and colon of the rats were examined, and correlations between AS and microbiota were analyzed. The aortic mesothelium of the experimental rats was damaged. The intima showed evident calcium salt deposition, indicating that the AS rat model was successfully developed. We noted varying degrees of pathological damage in the ileum and colon of the experimental rats. The 16S rDNA high-throughput sequencing showed significant differences in α-diversity, ß-diversity, and microbiota comparisons in the ileum and colon. Furthermore, the ileum and colon of AS rats showed varying degrees of intestinal microbiota disturbance. This article contributes to the study of the relationship between the microbiota in different regions of the gut and AS, and provides new approaches in gut microbiota intervention for the treatment of AS.


Assuntos
Aterosclerose , Microbiota , Animais , Aterosclerose/etiologia , Aterosclerose/patologia , Cálcio , Colo , DNA Ribossômico , Dieta Hiperlipídica/efeitos adversos , Íleo/patologia , Microbiota/fisiologia , Ratos
8.
J Oncol ; 2022: 7043431, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36281290

RESUMO

Gliomas are the most common tumor in the central nervous system with limited prognostic markers making it difficult to research progression. Induction of cellular immunogenic death is a promising treatment for glioma. Pyroptosis is one of the recently discovered programmed immuogenic cell death modes which remains unclear in glioma. We obtained glioma datasets from the CGGA and TCGA websites. Pearson correlation analysis was used to find pyroptosis-related lncRNAs. Subsequently, the univariate, LASSO, and multivariate Cox regression were applied to construct a prognostic signature based on pyroptosis-related lncRNAs. Kaplan-Meier plots, ROC curves, and PCA were utilized for testing the prognostic performance of the signature. We conducted the univariate and multivariate Cox regressions to ascertain if the signature worked as an independent factor for predicting overall survival (OS) for individuals with glioma from other characteristics. For evaluating the immune landscape differences between the subgroups, ESTIMATE, CIBERTSORT, and ssGSEA were adopted. Additionally, biological functions and pathways of DEGs were identified by KEGG and GO. We also screened potential drugs and measured sensitivities of chemotherapeutics between the subgroups by CellMiner and pRRophetic package. Finally, shRNA was conducted to knockdown of COX10-AS1 in U87 cells to determine its relationship with pyroptosis. We successfully created an effective pyroptosis-related lncRNA signature that divided individuals into groups of low- and high-risk, and individuals in the high-risk group were with poor prognosis in comparison to the individuals in the other group. A nomogram including clinical factors and risk scores to predict the OS was built. Furthermore, the two groups appeared to have different immune landscapes; the high-risk group showed greater levels of ESTIMATE scores, immune cell infiltration, and immune checkpoints. Additionally, immune-related pathways and functions were shown to be enriched according to KEGG and GO findings. Knockdown of COX10-AS1 inhibited U87 cell growth, upregulated CASP1 and NLRP3, and released more IL1-ß and IL-18 than the negative control. In summary, our study developed an lncRNA signature related to pyroptosis for OS prediction of gliomas and demonstrated its relationship with immune infiltration and drug sensitivity.

10.
Front Pharmacol ; 13: 989575, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36188536

RESUMO

Breast cancer (BC) is a kind of malignant cancer in women, and it has become the most diagnosed cancer worldwide since 2020. Histone methylation is a common biological epigenetic modification mediating varieties of physiological and pathological processes. Lysine-specific demethylase 1 (LSD1), a first identified histone demethylase, mediates the removal of methyl groups from histones H3K4me1/2 and H3K9me1/2 and plays a crucial role in varieties of cancer progression. It is also specifically amplified in breast cancer and contributes to BC tumorigenesis and drug resistance via both demethylase and non-demethylase manners. This review will provide insight into the overview structure of LSD1, summarize its action mechanisms in BC, describe the therapeutic potential of LSD1 inhibitors in BC, and prospect the current opportunities and challenges of targeting LSD1 for BC therapy.

11.
Food Funct ; 13(22): 11467-11475, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36193765

RESUMO

As a dietary supplement, supraphysiological levels of D-mannose have been proven to attenuate colitis via administration of 20% (w/v) D-mannose given in drinking water or by oral gavage. However, we observed that a lower dosage of 2% (w/v) D-mannose had no significant beneficial effect on colitis. Herein, enzymatic modification of mannose (phosphatidylmannoside, PtdMan) via phospholipase D catalyzed transphosphatidylation is proposed as a way to enhance the modulatory effect of mannose on colitis. We firstly demonstrated that phosphatidylmannoside showed a better preventive effect to alleviate the symptoms of colitis than D-mannose, as evidenced by higher body weight, lower disease activity index scores, and improvement of colonic pathological damage in vivo. Furthermore, our data highlight that PtdMan significantly attenuated pro-inflammatory cytokine levels in the colons of DSS-colitis mice and lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, and modulated M1/M2 polarization via PPARγ activation. Concomitantly, in comparison with mice treated with D-mannose, the PtdMan treatment regimen showed therapeutic efficacy against colitis after 5 days of DSS induction. In summary, PtdMan showed the potential to reduce inflammation and ameliorate intestinal damage in DSS-induced colitis. These results indicate that PtdMan attenuates DSS-induced colitis and LPS-induced inflammation, and the mechanism behind the phenomenon may be regulating macrophage polarization. Our study provides a theoretical basis for phosphatidylmannoside to be used as a potential candidate for the treatment of colitis.


Assuntos
Colite , PPAR gama , Camundongos , Animais , Sulfato de Dextrana/efeitos adversos , PPAR gama/genética , Manose , Lipopolissacarídeos/efeitos adversos , Colo/patologia , Colite/induzido quimicamente , Colite/tratamento farmacológico , Colite/patologia , Inflamação/patologia , Células RAW 264.7 , Camundongos Endogâmicos C57BL , Modelos Animais de Doenças
12.
Food Funct ; 13(22): 11744-11757, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36281730

RESUMO

Salecan, a natural ß-glucan consisting of seven residues linked by ß-(1→3)/α-(1→3) glycosidic bonds, is one of the novel food ingredients approved in China. ß-Glucan has a variety of health-improving effects, yet its mechanism against liver injury remains poorly understood. ß-Glucan can induce shifts in the gut microbiota and show health benefits; however, whether modulation of the gut microbiota by ß-glucan is associated with its benefits remains unclear. Here, the hepatoprotective effect and potential mechanism of salecan supplementation using a model of CCl4-induced liver injury were investigated. After 8 weeks of treatment, salecan alleviated liver injury by regulating oxidative stress and activating the Nrf2 signaling pathway. In addition, salecan treatment modulated the composition of the gut microbiota, and the antibiotic cocktail treatment indicated that the hepatoprotective effect of salecan was dependent on the gut microbiota. Fecal microbiota transplantation was used to further verify this mechanism, and we confirmed that microbial colonization partially alleviated liver injury. Besides, microbiota-derived metabolites of salecan also contributed to the hepatoprotective effect of salecan against liver injury and inhibited oxidative stress. These findings supported that salecan intervention attenuated liver injury by regulating the gut microbiota and its metabolites.


Assuntos
Microbioma Gastrointestinal , beta-Glucanas , Animais , Camundongos , beta-Glucanas/química , Fígado/metabolismo , Estresse Oxidativo , Camundongos Endogâmicos C57BL
13.
Biosens Bioelectron ; 217: 114709, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36115123

RESUMO

Osteosarcoma is one of the most frequent primary sarcoma of bone among adolescents. Early diagnosis of osteosarcoma is the key factor to achieve high survival rate of patients. Nevertheless, traditional histological biopsy is highly invasive and associated with the risk of arousing tumor spread. Herein, we develop a method integrating microfluidics and surface-enhanced Raman spectroscopy (SERS) to isolate plasma-derived exosomes and profile multiple exosomal biomarkers for the diagnosis of osteosarcoma. The method showed highly efficient isolation of exosomes directly from human plasma and can profile exosomes based on protein biomarkers, with the detection limit down to 2 exosomes per µL. The whole assay can be performed in 5 h and only consumed 50 µL of plasma for one analysis. With the method, we analyzed the level of three protein biomarkers, i.e., CD63, vimentin (VIM) and epithelial cell adhesion molecule (EpCAM), on plasma-derived exosomes from 20 osteosarcoma patients and 20 heathy controls. Significantly higher levels of CD63, VIM and EpCAM were observed on plasma exosomes from the osteosarcoma patients compared to the healthy controls. Based on the level of the exosomal biomarkers, a classification model was built for the rapid diagnosis of osteosarcoma, with the sensitivity, specificity and accuracy of 100%, 90% and 95%, respectively. The proposed method does not require complex operations nor expensive equipment, and has great promise in clinical diagnosis of cancer as a liquid biopsy technique.


Assuntos
Técnicas Biossensoriais , Neoplasias Ósseas , Exossomos , Osteossarcoma , Adolescente , Biomarcadores Tumorais , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/metabolismo , Molécula de Adesão da Célula Epitelial/análise , Exossomos/química , Humanos , Microfluídica/métodos , Osteossarcoma/diagnóstico , Osteossarcoma/metabolismo , Vimentina/análise , Vimentina/metabolismo
14.
Soft Matter ; 18(39): 7662-7669, 2022 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-36172725

RESUMO

As a toxic substance, ammonia can cause serious irritation to the human respiratory system and lungs. Although many detection techniques have been reported, most of them have drawbacks, such as expensive devices and complex and time-consuming fabrication processes. Thus, it is important to develop a simple method for ammonia detection. In this paper, we demonstrate a highly sensitive fluorescent sensor for ammonia detection based on aggregation-induced emission luminogen-doped liquid crystals without the use of polarizers. The homeotropic orientation of the liquid crystals on a modified substrate can be disturbed by ammonia, resulting in the fluorescence intensity change of an aggregation-induced emission luminogen. This aggregation-induced emission luminogen-doped liquid crystal-based fluorescent sensor for ammonia detection exhibited a low detection limit of 5.4 ppm, which is 3 times lower than previously reported liquid crystal-based optical sensors. The detection range is also broad from 0 ppm to 1600 ppm. Meanwhile, this sensor can be applied to detect aqueous ammonia with a low limit of detection of 1.8 ppm. The proposed fluorescent sensor for ammonia detection based on an aggregation-induced emission luminogen-doped liquid crystal is highly sensitive, highly selective, simple, and low cost with wide potential applications in chemical and biological fields. This strategy of designing a liquid crystal fluorescent sensor provides an inspiring stage for other toxic chemical substrates by changing specific decorated molecules.


Assuntos
Amônia , Cristais Líquidos , Corantes Fluorescentes/química , Humanos
15.
BMJ Open ; 12(9): e062206, 2022 09 29.
Artigo em Inglês | MEDLINE | ID: mdl-36175102

RESUMO

INTRODUCTION: Previous studies have demonstrated that one anastomosis gastric bypass (OAGB) is not inferior to Roux-en-Y gastric bypass (RYGB) in treating obesity. However, high level evidence comparing the efficacy and safety of both procedures in type 2 diabetes (T2D) treatment is still lacking, which is another main aim of bariatric surgery. The presented trial has been designed to aim at investigating the superiority of OAGB over the reference procedure RYGB in treating T2D as primary endpoint. And diabetes-related microvascular and macrovascular complications, cardiovascular comorbidities, weight loss, postoperative nutritional status, quality of life and overall complications will be followed up for 5 years as secondary endpoints. METHODS AND ANALYSIS: This prospective, multicentre, randomised superiority open-label trial will be conducted in patients of Asian descent. A total of 248 patients (BMI≥27.5 kg/m2) who are diagnosed with T2D will be randomly assigned (1:1) to OAGB or RYGB with blocks of four. The primary endpoint is the complete diabetes remission rate defined as HbA1c≤6.0% and fasting plasma glucose≤5.6 mmol/L without any antidiabetic medications at 1 year after surgery. All secondary endpoints will be measured at different follow-up visit points, which will start at least 3 months after enrolment, with a continuous annual follow-up for five postoperative years in order to provide solid evidence on the efficacy and safety of OAGB in patients with T2D. ETHICS AND DISSEMINATION: The study has been approved by the ethics committee of leading centre (Beijing Friendship Hospital, Capital Medical University, no. 2021-P2-037-03). The results generated from this work will be disseminated to academic audiences and the public via publications in international peer-reviewed journals and conferences. The data presented will be imported into a national data registry. Findings are expected to be available in 2025, which will facilitate clinical decision-making in the field. TRIAL REGISTRATION NUMBER: NCT05015283.


Assuntos
Diabetes Mellitus Tipo 2 , Derivação Gástrica , Glicemia , Diabetes Mellitus Tipo 2/cirurgia , Hemoglobina A Glicada , Humanos , Hipoglicemiantes/uso terapêutico , Estudos Multicêntricos como Assunto , Estudos Prospectivos , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto
16.
Crit Rev Food Sci Nutr ; : 1-9, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36069291

RESUMO

Objective: Fasting is considered to be a food structure that can improve body health. Several randomized clinical trials (RCTs) have investigated the effects of fasting in patients with metabolic syndrome (MS). In this review, we performed a meta-analysis to assess the effects of fasting on patients with MS. Methods: Following PRISMA guidelines, a systematic literature search was conducted in PubMed, Embase, and Cochrane Central updated to September 2021. The quality evaluation and heterogeneity detection of the included research literature were carried out by Revman and Stata software through a random-effects model. Results: A total of 268 subjects were included. The pooled results revealed that fasting significantly reduced body weight (WMD: -2.48 kg, 95% CI: -3.22, -1.74), BMI (WMD = -2.72 cm; 95%CI: -4.04, -1.40 cm), body fat percent (WMD: -1.57%, 95%CI: -2.47, -0.68), insulin level (WMD: -2.45 mmol/L; 95%CI: -4.40, -0.49 mmol/L) and HOMA-IR (WMD:-0.65 mmol/L; 95%CI: -0.90, -0.41 mmol/L) in patients with MS, whereas had no effect on glucose, blood pressure and lipids profile. Conclusions: Our findings provide insights into the effect of fasting on the anthropometric outcomes, insulin resistance, and gut microbiota in MS.

17.
Front Oncol ; 12: 993775, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119505

RESUMO

Breast cancer (BC) is a common malignancy that mainly occurred in women and it has become the most diagnosed cancer annually since 2020. Berberine (BBR), an alkaloid extracted from the Berberidacea family, has been found with broad pharmacological bioactivities including anti-inflammatory, anti-diabetic, anti-hypertensive, anti-obesity, antidepressant, and anticancer effects. Mounting evidence shows that BBR is a safe and effective agent with good anticancer activity against BC. However, its detailed underlying mechanism in BC treatment remains unclear. Here, we will provide the evidence for BBR in BC therapy and summarize its potential mechanisms. This review briefly introduces the source, metabolism, and biological function of BBR and emphasizes the therapeutic effects of BBR against BC via directly interacting with effector proteins, transcriptional regulatory elements, miRNA, and several BBR-mediated signaling pathways. Moreover, the novel BBR-based therapeutic strategies against BC improve biocompatibility and water solubility, and the efficacies of BBR are also briefly discussed. Finally, the status of BBR in BC treatment and future research directions is also prospected.

19.
Stem Cell Res Ther ; 13(1): 401, 2022 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-35932080

RESUMO

INTRODUCTION: The basis of orthodontic tooth movement (OTM) is the reconstruction of periodontal tissue under stress. Increasing the speed of OTM has always been the focus of attention. OBJECTIVES: Periodontal ligament stem cells (PDLSCs) are direct effector cells of mechanical force, but the mechanism by which PDLSCs sense mechanical stimuli is unclear. METHODS: Human PDLSCs (hPDLSCs) were analyzed in the presence or absence of force loading with the Flexcell loading system in vitro. Then, periodontal tissues were analyzed after mechanical stimulation in vivo. In addition, cells in a confined microenvironment were analyzed to observe changes in the cytoskeleton and migration. Finally, TRPC6-/- mice were used to further verify the effect of TRPC6. After force application, the OTM distance, bone marrow density (BMD), TRPC6 and COL1 expression, and TRAP staining were evaluated in periodontal tissues. RESULTS: RNA sequencing (RNA-seq) and western blot analyses revealed that TRPC6 was important during mechanical force application to hPDLSCs. Appropriate mechanical force application also induced TRPC6 activation in the OTM model and the confined microenvironment. Under a slightly confined microenvironment, treatment with the TRPC6 inhibitor SKF96365 and TRPC6 knockout decreased the migration speed of hPDLSCs and mouse bone marrow mesenchymal stem cells (mBMSCs). In addition, TRPC6-/- mice showed lower OTM distances and reduced osteogenic and osteoclastic differentiation. CONCLUSION: In summary, TRPC6 activation in PDLSCs mediated by appropriate mechanical force application contributes to periodontal tissue reconstruction. PDLSCs modulate periodontal tissue remodeling under appropriate mechanical stimulation through TRPC6; however, under excessive stress, alveolar bone and tooth roots are readily absorbed. Under this condition, environmental factors play a leading role, and the regulatory effect of TRPC6 is not obvious.


Assuntos
Células-Tronco Mesenquimais , Ligamento Periodontal , Animais , Diferenciação Celular/fisiologia , Humanos , Células-Tronco Mesenquimais/metabolismo , Camundongos , Osteogênese/fisiologia , Células-Tronco/metabolismo , Canal de Cátion TRPC6/metabolismo , Técnicas de Movimentação Dentária
20.
Biosensors (Basel) ; 12(8)2022 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-36004973

RESUMO

As an emerging stimuli-responsive material, liquid crystal (LC) has attracted great attentions beyond display applications, especially in the area of biochemical sensors. Its high sensitivity and fast response to various biological or chemical analytes make it possible to fabricate a simple, real-time, label-free, and cost-effective LC-based detection platform. Advancements have been achieved in the development of LC-based sensors, both in fundamental research and practical applications. This paper briefly reviews the state-of-the-art research on LC sensors in the biochemical field, from basic properties of LC material to the detection mechanisms of LC sensors that are categorized into LC-solid, LC-aqueous, and LC droplet platforms. In addition, various analytes detected by LCs are presented as a proof of the application value, including metal ions, nucleic acids, proteins, glucose, and some toxic chemical substances. Furthermore, a machine-learning-assisted LC sensing platform is realized to provide a foundation for device intelligence and automatization. It is believed that a portable, convenient, and user-friendly LC-based biochemical sensing device will be achieved in the future.


Assuntos
Técnicas Biossensoriais , Cristais Líquidos , Glucose , Íons , Cristais Líquidos/química , Água/química
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