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1.
Sensors (Basel) ; 21(16)2021 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-34451003

RESUMO

The mechanical properties of biological cells, especially the elastic modulus and viscosity of cells, have been identified to reflect cell viability and cell states. The existing measuring techniques need additional equipment or operation condition. This paper presents a cell's viscoelasticity measurement method based on the spheroidization process of non-spherical shaped cell. The viscoelasticity of porcine fetal fibroblast was measured. Firstly, we introduced the process of recording the spheroidization process of porcine fetal fibroblast. Secondly, we built the viscoelastic model for simulating a cell's spheroidization process. Then, we simulated the spheroidization process of porcine fetal fibroblast and got the simulated spheroidization process. By identifying the parameters in the viscoelastic model, we got the elasticity (500 Pa) and viscosity (10 Pa·s) of porcine fetal fibroblast. The results showed that the magnitude of the elasticity and viscosity were in agreement with those measured by traditional method. To verify the accuracy of the proposed method, we imitated the spheroidization process with silicone oil, a kind of viscous and uniform liquid with determined viscosity. We did the silicone oil's spheroidization experiment and simulated this process. The simulation results also fitted the experimental results well.


Assuntos
Técnicas de Imagem por Elasticidade , Animais , Simulação por Computador , Módulo de Elasticidade , Elasticidade , Suínos , Viscosidade
2.
Ultrason Sonochem ; 77: 105668, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34298307

RESUMO

To extend the shelf life and retain bioactive proteins in milk, this study utilized microfiltration (MF) combined with ultrasonication to treat skim milk and investigated its efficiency in removing bacteria and retaining bioactive proteins compared with HTST pasteurization and microfiltration alone. Results showed that microfiltration combined with ultrasonication at 1296 J/mL could completely remove the bacteria in skim milk. Ultrasonication further extended the shelf life (4 °C) of microfiltered skim milk, which could reach at least 40 days when MF was combined with ˃1296 J/mL ultrasonication. In addition, ELISA showed that HTST pasteurization significantly decreased the levels of IgG by ~30%, IgA by ~ 50%, IgM by ~60%, and lactoferrin by ~40%, whereas the activity of the enzymes lactoperoxidase and xanthine oxidase were also decreased by ~ 20%. Compared with HTST, MF alone or combined with ultrasonication retained these bioactive proteins to a larger degree. On the other hand, proteomics indicated both damage to casein micelle and fat globule structures in milk when ultrasonication at >1296 J/mL was applied, as shown by increases in caseins and milk fat globular proteins. Simultaneously, this ultrasound intensity also decreased levels of bioactive proteins, such as complement factors. Taken together, this study provided new insights that may help to implement this novel combination of non-thermal technologies for the dairy industry aimed at improving milk quality and functionality.


Assuntos
Filtração , Armazenamento de Alimentos , Leite/química , Proteínas/química , Sonicação , Animais , Temperatura
3.
Sci Total Environ ; 752: 142225, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33207503

RESUMO

Methane (CH4) is one of the most important greenhouse gases which can be formed by methanogens and oxidized by methanotrophs, as well as ammonia oxidizers. Agricultural soils can be both a source and sink for atmospheric CH4. However, it is unclear how climate change, will affect CH4 emissions and the underlying functional guilds. In this field study, we determined the impact of simulated climate change (a warmer and drier condition) and its legacy effect on CH4 emissions and the methanogenic and methanotrophic communities, as well as their relationships with ammonia oxidizers in an acidic soil with urea application. The climate change conditions were simulated in a greenhouse, and the legacy effect was simulated by removing the greenhouse after twelve months. Simulated climate change significantly decreased the in situ CH4 emissions in the urea-treated soils while the legacy effect significantly decreased the in situ CH4 emissions in the control plots, but had very little effect in the urea-treated soils. This indicates that the impact of simulated climate change and its legacy on CH4 emissions was significantly modified by nitrogen fertilization. Methanotrophs were more sensitive than methanogens in response to simulated climate change and its legacy effect, especially in the urea treated soil. Significant negative correlations were observed between the abundances of ammonia oxidizers and methanotrophs. Additionally, results of partial least path modeling (PLS-PM) indicated that the interactions of methanogens and methanotrophs with ammonia oxidizing archaea (AOA) had significant positive relationships with in situ CH4 emissions under the simulated climate change condition. Our work highlights the important role of AOA for CH4 emissions under climate change conditions. Further research is needed to better understand this effect in other ecosystems.


Assuntos
Metano , Solo , Amônia , Mudança Climática , Ecossistema , Microbiologia do Solo
4.
IEEE Trans Biomed Eng ; PP2020 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-33156778

RESUMO

OBJECTIVE: The invisibility of domestic oocyte nucleus in bright field currently forces operators to blindly aspirate nucleus out in oocyte enucleation, usually causing large cytoplasm losses and poor developmental competences of cloned embryos. Although fluorescent labeling of nucleus allows for nucleus localization, the involved photobleaching problems and barriers to the execution of enucleation process limit its online-application in oocyte enucleation. This paper reports a novel label-free oocyte enucleation method for precise removal of the nucleus with less cytoplasm loss. METHODS: The relative positions between the injection pipette and nucleus for complete removal of nucleus with less cytoplasm loss were determined through a finite element modeling of nucleus aspiration. To position injection pipette to the above positions relative to nucleus, the appropriate oocyte orientation and trajectory of injection pipette inside oocyte were derived according to the offline-calibrated 3-D distribution of nucleus and the simulated dynamic drift of nucleus that occurs as injection pipette is maneuvered inside oocyte. Finally, a robotic label-free precise enucleation procedure was established. RESULTS: The experimental results on more than 1000 porcine oocytes proved that this system is capable of reducing cytoplasm loss by 60% at the same level of enucleation success rate and almost doubling the cleavage rate of clone embryos in comparison to blind aspiration method. CONCLUSIONS: The results prove that our method significantly improves the developmental competence of cloned embryos in comparison to manual enucleation method. SIGNIFICANCE: Our method is expected to improve the extremely low success rate of animal cloning in the future.

5.
Food Chem ; 314: 126177, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31954943

RESUMO

This study mainly investigated changes in milk serum proteins by simulating the industrial processing of whole milk powder using a label-free proteomic approach. A total of 391 proteins were identified, 89 of which were quantified in all groups. Compared with raw milk, the milk subjected to the heating pasteurization process showed significantly decreased the serum protein profile, whereas that subjected to vacuum concentration and spray-drying showed minimal changes. The LC-MS/MS results were further confirmed by determining the activity of bioactive xanthine oxidase and retention of lactoferrin and immunoglobulins. The data showed that 70%-85% of lactoferrin and immunoglobulins were retained in vacuum-concentrated or spray-dried milk but were hardly identifiable in heat-pasteurized milk or whole milk powders. These findings indicate the need for improving the current milk powder-manufacturing techniques to allow the retention of active milk proteins.


Assuntos
Manipulação de Alimentos/métodos , Proteínas do Leite/química , Leite/química , Pós/química , Animais , Cromatografia Líquida , Dessecação , Temperatura Alta , Imunoglobulinas/análise , Imunoglobulinas/química , Lactoferrina/análise , Lactoferrina/química , Proteínas do Leite/análise , Pasteurização , Proteômica , Espectrometria de Massas em Tandem , Xantina Oxidase/análise , Xantina Oxidase/química
6.
J Food Sci ; 84(6): 1447-1455, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31116427

RESUMO

Oleogel with tailored viscoelasticity is a great interest for food structuring, while its potential benefits for edible film performance are not clear. In this study, ß-sitosterol (0, 5, 10, 15, and 20 wt%)-corn oil oleogel was developed and used in the formation of gelatin-based films. Importantly, adding oleogel significantly decreased water vapor permeability of the gelatin films, however, it had little negative influence on film strength. In addition, the results of this study demonstrated that increasing the sitosterol in oleogels led to an increasing number of ordered crystals formed in the oleogel, which contributed to compact and smooth surface of the film. Moreover, the incorporation of oleogel also caused some changes in molecule conformation and film barrier property. Therefore, the superior mechanical performance and moisture resistance properties of the film were obtained when 15% ß-sitosterol was used to prepare oleogel. PRACTICAL APPLICATION: Corn oil oleogels ß-sitosterol was incorporated with gelatin to prepare the gelatin film aiming to improve the water resistance of the films for its variety of practical production. The enhanced vapor permeability and accepted strength of the emulsion film indicated the potential application of it with a variety of edible packaging forms, such as films, pouch and sachet in medium and high humid condition.


Assuntos
Óleo de Milho/química , Embalagem de Alimentos/instrumentação , Gelatina/química , Sitosteroides/administração & dosagem , Água/química , Embalagem de Alimentos/métodos , Compostos Orgânicos/química , Permeabilidade , Vapor , Viscosidade
7.
Micromachines (Basel) ; 10(5)2019 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-31137867

RESUMO

As there are significant variations of cell elasticity among individual cells, measuring the elasticity of batch cells is required for obtaining statistical results of cell elasticity. At present, the micropipette aspiration (MA) technique is the most widely used cell elasticity measurement method. Due to a lack of effective cell storage and delivery methods, the existing manual and robotic MA methods are only capable of measuring a single cell at a time, making the MA of batch cells low efficiency. To address this problem, we developed a robotic MA system capable of storing multiple cells with a feeder micropipette (FM), picking up cells one-by-one to measure their elasticity with a measurement micropipette (MM). This system involved the following key techniques: Maximum permissible tilt angle of MM and FM determination, automated cell adhesion detection and cell adhesion break, and automated cell aspiration. The experimental results demonstrated that our system was able to continuously measure more than 20 cells with a manipulation speed quadrupled in comparison to existing methods. With the batch cell measurement ability, cell elasticity of pig ovum cultured in different environmental conditions was measured to find optimized culturing protocols for oocyte maturation.

8.
Micromachines (Basel) ; 10(2)2019 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-30781803

RESUMO

Polar-body detection is an essential and crucial procedure in various automatic cell manipulations. The polar body can only be observed when it is located near the focal plane of the microscope, so we need to detect the polar body during cell rotation in cell manipulations. However, three-dimensional cell rotation by micropipette causes polar-body defocus and cell/polar-body deformation, which have not been discussed in existing image-level polar-body-detection approaches. Moreover, varying sizes of the polar bodies increase the difficulty of polar-body detection. In this paper, we propose a deep-learning-based framework to realize polar-body detection in cell rotation. The detection problem is interpreted as image segmentation, which separates the polar body from the background. Then, we improve U-net, which is a typical convolutional neural network (CNN) for medical-image segmentation, so that the network can be applied to polar-body detection, especially for the detection of defocused polar bodies and polar bodies of different sizes. For CNN training, we also designed a particular image-transformation method to simulate more cell-rotation situations, including cell- and polar-body deformation, so that the deformed polar body in cell rotation would be detected by the proposed method. Experiment results show that our method achieves high detection accuracy of 98.7% on a test dataset of 1000 images, and performs well in cell-rotation processes. This method can be applied to various automatic cell manipulations in the future.

9.
Micromachines (Basel) ; 9(4)2018 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-30424075

RESUMO

It is essential to have three-dimensional orientation of cells under a microscope for biological manipulation. Conventional manual cell manipulation is highly dependent on the operator's experience. It has some problems of low repeatability, low efficiency, and contamination. The current popular robotic method uses an injection micropipette to rotate cells. However, the optimal poking direction of the injection micropipette has not been established. In this paper, a strategy of robotic cell rotation based on optimal poking direction is proposed to move the specific structure of the cell to the desired orientation. First, analysis of the force applied to the cell during rotation was done to find the optimal poking direction, where we had the biggest moment of force. Then, the moving trajectory of the injection micropipette was designed to exert rotation force based on optimal poking direction. Finally, the strategy was applied to oocyte rotation in nuclear transfer. Experimental results show that the average completion time was up to 23.6 s and the success rate was 93.3% when the moving speed of the injection micropipette was 100 µm/s, which demonstrates that our strategy could overcome slippage effectively and with high efficiency.

10.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 26(4): 1151-1155, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30111422

RESUMO

OBJECTIVE: To analyze the number of myeloid-derived suppressor cells(MDSC) and the level of prostaglandin E2(PGE2) in the bone marrow of adult ITP patients, and to explore their possible mechanisms involved in the pathogenesis of this disease. METHODS: Twenty-five patients of newly diagnosed ITP, 25 patients of complete remission group and 15 patients of control group were selected. The number of MDSC in the bone marrow between 3 groups was detect by flow cytometry (FCM). The serum level of prostaglandin E2 (PGE2) in 3 groups was determined by enzyme linked immunosorbent assay (ELISA). The relative expression of IFN-γ mRNA in bone marrow mononuclear cells was measured by real time fluorescence quantitative polymerase chain reaction (RT-qPCR) in each groups. RESULTS: The number of MDSC in the complete remission group was significantly higher than that in the control group (P<0.05); the number of MDSC in the newly diagnosed group was higher than that in the control group; the number of MDSC in the complete remission group was higher than that in the newly diagnosed group. The serum level of PGE2 in bone marrow of ITP patients in the newly diagnosed group was higher than that of the control group(P<0.05). The serum level of PGE2 in the bone marrow of ITP patients of the complete remission group was higher than that of the control group (P<0.05). The level of PGE2 in bone marrow serum of ITP patients of the newly diagnosed group was lower than that in the complete remission group(P<0.05). The relative expression level of IFN-gamma in bone marrow mononuclear cells of the ITP patients in newly diagnosed group was higher than that in the control group and the complete remission group(P<0.001). The relative quantification (RQ) of IFN-γ in bone marrow mononuclear cells was 2.60 between the newly diagnosed group and the complete remission group. CONCLUSION: When adult ITP disease is remitted, the number of MDSC rises and correlates with the therapeutic response and PGE2 level in the bone marrow.


Assuntos
Células Supressoras Mieloides , Adulto , Medula Óssea , Citometria de Fluxo , Humanos , Púrpura Trombocitopênica Idiopática , RNA Mensageiro
11.
Sci Total Environ ; 634: 1157-1164, 2018 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-29660872

RESUMO

Nitrifying microbes are of critical importance in regulating efficient nitrogen (N) cycling, which plays a crucial role in plant productivity and maintaining soil sustainability. Long-term different intensities of grazing can strongly influence the microbial communities, while our understanding of the complex nitrifying community in the grazed grassland soil environment is still limited. To investigate whether and how long-term grazing with different intensities influence soil nitrifying communities, high-throughput sequencing and quantitative PCR analyses were performed on soil samples from permanent grassland soils under four grazing intensities: 0 (G0), 1.5 (G1), 6 (G2) and 9 (G3) sheepha-1. Results showed that the G3 treatment significantly reduced the soil nutrient content and increased the soil bulk density, changes that are not sustainable in the long run. The G1 treatment, on the other hand, significantly increased the soil nutrient content and would improve soil fertility. Some functional microbes were specifically enriched after long term grazing, like Nitrospirae (phylum) to Nitrospira (class) in the G2 samples and Chromatiales (order) to Nitrosococcus (genus) in the G3 soils. The numerically dominant Nitrosococcus watsonii lineage of ammonia oxidizing bacteria (AOB) was observed in this grassland soil. The redundancy analysis (RDA) together with the structural equation modeling (SEM) analysis showed that grazing intensity was important in mediating the distribution of soil microorganisms and affected nitrifying communities by impacting soil physicochemical characteristics (e.g., bulk density, NH4+-N). These results showed the shifts of nitrifying communities across different grazing intensities, and could aid in the determination of an optimal grazing intensity for these grazed grassland soils.


Assuntos
Monitoramento Ambiental , Pradaria , Herbivoria , Nitrificação , Microbiologia do Solo
12.
Water Res ; 115: 130-137, 2017 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-28273443

RESUMO

The effects of nitrate (NO3-) on chromate (Cr(VI)) reduction in a membrane biofilm reactor (MBfR) were studied when CH4 was the sole electron donor supplied with a non-limiting delivery capacity. A high surface loading of NO3- gave significant and irreversible inhibition of Cr(VI) reduction. At a surface loading of 500 mg Cr/m2-d, the Cr(VI)-removal percentage was 100% when NO3- was absent (Stage 1), but was dramatically lowered to < 25% with introduction of 280 mg N m-2-d NO3- (Stage 2). After ∼50 days operation in Stage 2, the Cr(VI) reduction recovered to only ∼70% in Stage 3, when NO3- was removed from the influent; thus, NO3- had a significant long-term inhibition effect on Cr(VI) reduction. Weighted PCoA and UniFrac analyses proved that the introduction of NO3- had a strong impact on the microbial community in the biofilms, and the changes possibly were linked to the irreversible inhibition of Cr(VI) reduction. For example, Meiothermus, the main genus involved in Cr(VI) reduction at first, declined with introduction of NO3-. The denitrifier Chitinophagaceae was enriched after the addition of NO3-, while Pelomonas became important when nitrate was removed, suggesting its potential role as a Cr(VI) reducer. Moreover, introducing NO3- led to a decrease in the number of genes predicted (by PICRUSt) to be related to chromate reduction, but genes predicted to be related to denitrification, methane oxidation, and fermentation increased.


Assuntos
Biofilmes , Cromatos , Reatores Biológicos , Metano , Nitratos , Oxirredução
13.
Int J Biol Macromol ; 98: 292-301, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28163130

RESUMO

The inferior thermal- stability of collagen hinders its extensive application in food industry, including edible packaging. To improve the thermal- stability and mechanical properties of collagen, we attempted to crosslink collagen with some proteins possessing excellent thermal stability (i. e., casein, keratin and soy protein isolate (SPI)). Observed from the SDS- PAGE and particle size distribution, some complexes with higher molecule weight and relative bigger size particle occurred in the protein mixture, especially after TGase crosslinking. Importantly, the crosslinking greatly improved the thermal- stable property of protein complex, especially that of the collagen- casein complex judged from differential scanning calorimetric (DSC). Moreover, the crosslinking enhanced the mechanical properties of the combined films in terms of tensile strength (TS) and elongation at break (EAB). Also, some obvious differences in morphology of proteins before and after TGase crosslinking were observed by scanning electron microscopy (SEM). These impacts of TGase crosslinking with heat- resistant proteins on collagen features were associated with the conformational changes of the protein complex analyzed by Fourier transform infrared spectroscopy (FTIR). In conclusion, TGase crosslinking with higher thermally stable proteins could be an effective method to contribute to collagen' application in food packaging field.


Assuntos
Caseínas/química , Colágeno Tipo I/química , Queratinas/química , Proteínas de Soja/química , Temperatura , Resistência à Tração , Transglutaminases/metabolismo , Tamanho da Partícula , Estabilidade Proteica
14.
J Sci Food Agric ; 97(11): 3613-3622, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28102016

RESUMO

BACKGROUND: In order to obtain new reinforcing bio-fillers to improve the physicochemical properties of gelatin-based films, three types of maize starch, waxy maize starch (Ap), normal starch (Ns) and high-amylose starch (Al), were incorporated into gelatin film and the resulting film properties were investigated, focusing on the impact of amylose content. RESULTS: The thickness, opacity and roughness of gelatin film increased depending on the amylose content along with the starch concentration. The effects of the three starches on the mechanical properties of gelatin film were governed by amylose content, starch concentration as well as environmental relative humidity (RH). At 75% RH, the presence of Al and Ns in the gelatin matrix increased the film strength but decreased its elongation, while Ap exhibited an inverse effect. Starch addition decreased the oxygen permeability of the film, with the lowest value at 20% Al and Ns. All starches, notably at 30% content, led to a decrease in the water vapor permeability of the film at 90% RH, especially Ns starch. Furthermore, the starches improved the thermal stability of the film to some extent. Fourier transform infrared spectra indicated that some weak intermolecular interactions such as hydrogen bonding occurred between gelatin and starch. Moreover, a high degree of B-type crystallinity of starch was characterized in Gel-Al film by X-ray diffraction. CONCLUSION: Tailoring the properties of gelatin film by the incorporation of different types of maize starch provides the potential to extend its applications in edible food packaging. © 2017 Society of Chemical Industry.


Assuntos
Amilose/química , Embalagem de Alimentos/instrumentação , Gelatina/química , Amido/química , Zea mays/química , Fenômenos Biomecânicos , Permeabilidade , Difração de Raios X
15.
Food Chem ; 216: 209-16, 2017 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-27596411

RESUMO

This study investigated the possibility of enhancing the properties of collagen with three different maize starches: waxy maize starch, normal starch, and high amylose starch. Scanning electron microscopy images revealed that starch-collagen films had a rougher surface compared to pure collagen films which became smoother upon heating. Amylose starch and normal starch increased the tensile strength of unheated collagen films in both dry and wet states, while all starches increased tensile strength of collagen film by heating. Depending upon the amylose content and starch concentrations, film solubility in water decreased with the addition of starch. DSC thermograms demonstrated that addition of all starches improved the thermal stability of the collagen film. Moreover, X-ray diffraction results indicated that except for high amylose starch, the crystallinity of both starch and collagen was significantly decreased when subject to heating. FTIR spectra indicated that intermolecular interactions between starch and collagen were enhanced upon heating.


Assuntos
Colágeno/química , Amido/química , Zea mays/química , Microscopia Eletrônica de Varredura , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier , Resistência à Tração , Difração de Raios X
16.
Int J Biol Macromol ; 94(Pt A): 258-265, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27732878

RESUMO

In order to study the impact of starch in film performance, high amylose corn starch was composited in gelatin films under different gelatinization conditions and, in high and low concentrations (10 and 50wt.%). It was found that hot water gelatinized starch (Gel-Shw) increased film mechanical strength and was dependent upon the starch concentration. The addition of an alkali component to the starch significantly enhanced the swelling of the starch granules and expedited the gelatinization process. Incorporation of starch, especially the alkalized starch (Sha), into the gelatin films decreased film solubility which improved its water resistance and water vapor permeability (WVP). Multiple techniques (DSC, TGA, FT-IR, and XRD) were used to characterize the process and results, including the crosslinking of the dissolved starch molecules and the particles formed from gelatinized starch during retrogradation process, which played an important role in improving the thermal stability of the composited gelatin films. Overall, the starch-gelatin composition provides a potential approach to improve gelatin film performance and benefit its applications in the food industry.


Assuntos
Amilose/química , Gelatina/química , Amido/química , Permeabilidade , Polimerização , Solubilidade , Propriedades de Superfície , Resistência à Tração
17.
Pain Med ; 16(8): 1622-8, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25800546

RESUMO

OBJECTIVES: To examine and compare the reliability and validity of two observational pain assessment tools used in a sample of Chinese critically ill patients. DESIGN: A prospective observational study. SETTING AND SUBJECTS: A convenience sample of 117 adult critically ill patients was recruited from a general Intensive Care Unit in a university-affiliated hospital in China. METHODS: Pain was assessed before and during painful and nonpainful routine care procedures (suctioning and noninvasive blood pressure measurement, respectively) using the Critical-Care Pain Observation Tool (CPOT) and the Behavioral Pain Scale (BPS), including the original BPS and the BPS for Non-Intubated patients (BPS-NI). Internal consistency, test-retest reliability, interrater reliability, and discriminant validity of the CPOT and the BPS were evaluated, and the two scales compared. RESULTS: A total of 608 assessments were obtained using the CPOT and the BPS. Overall Cronbach's alpha coefficient for the CPOT and the BPS was 0.795 and 0.791, respectively. Test-retest reliability of the CPOT and the BPS was 0.950 and 0.941, respectively. Overall weighted κ between the two raters of the CPOT and the BPS was 0.973 and 0.955, respectively. Scores of the CPOT and the BPS during the painful procedures were both significantly higher than those during the nonpainful procedures, and those at rest before the painful procedures. There was a strong correlation between the two scales with adequate limits of agreement. CONCLUSION: Both the CPOT and the BPS are reliable and valid tools to assess pain in intubated and nonintubated critically ill Chinese patients.


Assuntos
Estado Terminal/terapia , Medição da Dor/métodos , APACHE , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Grupo com Ancestrais do Continente Asiático , Comportamento , Cuidados Críticos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Observação , Variações Dependentes do Observador , Estudos Prospectivos , Reprodutibilidade dos Testes , Adulto Jovem
18.
J Clin Nurs ; 24(1-2): 212-21, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25236497

RESUMO

AIMS AND OBJECTIVES: To evaluate the reliability and validity of the Pain Assessment in Advanced Dementia scale and the Checklist of Nonverbal Pain Indicators in Chinese older adults post surgery during the anaesthesia recovery period. BACKGROUND: Pain assessment in older surgical patients is complicated by factors such as anaesthesia and opioid administration. Although observational pain behavioural assessment tools have been validated for those unable to self-report, research on their application during the anaesthesia recovery period is limited. DESIGN: A prospective correlational design. METHODS: Ninety-three older patients admitted for scheduled abdominal surgery were recruited in a university-affiliated hospital. The two observational scales were used to conduct pain assessments during the anaesthesia recovery period. On the first and the third postoperative day, participants recalled their pain intensity during the recovery period using the Numeric Rating Scale or the Faces Pain Scale-Revised. RESULTS: The internal consistency reliability of the Pain Assessment in Advanced Dementia scale and the Checklist of Nonverbal Pain Indicators was 0·81 and 0·69 respectively. The correlation between scores of the two observational scales was 0·95. The recalled self-reports of pain intensity were significantly correlated. The correlation of the Pain Assessment in Advanced Dementia scale and patients' recalled self-reports was 0·55, 0·54, and the correlation between the Checklist of Nonverbal Pain Indicators and the two recalled pain scores was both 0·60. CONCLUSIONS: Both the two observational scales had good reliability and validity when used to assess pain in Chinese surgical older adults during the anaesthesia recovery period. RELEVANCE TO CLINICAL PRACTICE: Observational pain scales can be useful as a tool for patients unable to self-report. Accurate use of one of the observational pain tools can help identify pain during the anaesthesia recovery period, when patients are unable to self-report, to support effective pain management during this period.


Assuntos
Abdome/cirurgia , Medição da Dor , Dor Pós-Operatória/diagnóstico , Idoso , Idoso de 80 Anos ou mais , Período de Recuperação da Anestesia , Grupo com Ancestrais do Continente Asiático , China , Demência/psicologia , Feminino , Hospitalização , Humanos , Masculino , Rememoração Mental , Pessoa de Meia-Idade , Dor Pós-Operatória/etiologia , Dor Pós-Operatória/psicologia , Estudos Prospectivos , Reprodutibilidade dos Testes , Autorrelato
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