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1.
Carbohydr Polym ; 227: 115345, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31590868

RESUMO

Chitin is the second most abundant natural polysaccharide with biocompatibility, biodegradability, and bioactivity. Homogeneous modification of chitin is an efficient way to improve or to impart new properties to chitin. Here, amide-modified ß-chitin (AMC), hydroxyethyl ß-chitin (HEC), and hydroxybutyl ß-chitin (HBC) through Michael addition, Williamson reaction, and ring-opening addition, were homogeneously synthesized from aqueous KOH/urea solution, respectively, with controlled structures and uniform properties. The reactions mainly occur at the hydroxyl groups of C-6 positions of ß-chitin chains due to mild conditions, and the obtained ß-chitin derivatives with high DS values are water-soluble. AMC could transform from sol to gel at acidic condition or upon adding Fe3+ due to the presence of partial carboxylate groups. As a specific highlight, HEC and HBC could thermally form smart hydrogels at physiological temperature, with cytocompatibility and blood biocompatibility, which is very useful as drug/ cell carriers for biomedical applications. The remarkably mild and "green" condition of aqueous KOH/urea solution for the synthesis of chitin derivatives has pioneered a better way to exploit its great diversity of the natural, sustainable polysaccharide.

2.
Food Chem ; 305: 125429, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31505415

RESUMO

A simple and rapid magnetic solid-phase extraction (MSPE) method using PEGylated multi-walled carbon nanotubes magnetic nanoparticles (PEG-MWCNTs-MNP) as absorbents is proposed for isolation and enrichment of aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), aflatoxin M1 (AFM1), aflatoxin M2 (AFM2), ochratoxin A (OTA), zearalenone (ZEA), zearalanone (ZAN), α-zeralanol (α-ZAL), ß-zeralanol (ß-ZAL), α-zeralenol (α-ZOL), and ß-zeralenol (ß-ZOL) from liquid milk. Combined with ultra-high performance liquid chromatography Q-Exactive high resolution mass spectrometry, simultaneous qualification of these mycotoxins was achieved with sensitivity and specificity. The proposed method showed a good linearity (R2 ≥ 0.995), high sensitivity (limit of detection in the range of 0.005-0.050 µg/kg and limit of quantification in the range of 0.015-0.150 µg/kg), adequate recovery (81.8-106.4%), and good repeatability (intra-day precision in the range of 2.1-8.5% and inter-day precision in the range of 3.9-11.7%). It has been successfully applied to the determination of 13 mycotoxins in real liquid milk samples.

3.
Toxicol Lett ; 318: 12-21, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31622651

RESUMO

Maternal smoking during pregnancy and lactation is associated with increased fat mass in the offspring, but the mechanism by which this occurs is not fully understood. Our study focused on the relationships among maternal nicotine exposure, adipose angiogenesis and adipose tissue function in female offspring. Pregnant rats were randomly assigned to nicotine or control groups. Microvascular density, lipid metabolism and α7nAChR-Egr1-FGF2 signaling pathway genes/proteins were tested in 4-, 12- and 26-week female offspring. In vitro, nicotine concentration- and time-response experiments were conducted in 3T3-L1. Lipid metabolism and α7nAChR-Egr1-FGF2 signaling pathway genes/proteins were tested. The conditioned media of differentiated 3T3-L1 treated with nicotine were used to observe tube formation in human umbilical vein endothelial cells (HUVECs). Nicotine-exposed females presented higher adipose microvascular density. The gene expression of α7nAChR, Egr1 and FGF2 was significantly increased in gonadal white adipose tissue (gWAT) and inguinal subcutaneous WAT (igSWAT) of nicotine-exposed females at 4 weeks of age. The protein expression of α7nAChR, Egr1 and FGF2 was increased in gWAT and igSWAT of nicotine-exposed females at 4 weeks of age, and increased in gWAT at 26 weeks. In vitro, nicotine increased the expression of lipid metabolism and α7nAChR-Egr1-FGF2 signaling pathway genes/proteins in a concentration- and time-dependent manner. In the tube formation experiment, adipocytes affected by nicotine promoted HUVEC angiogenesis. Therefore, maternal nicotine exposure promoted the early angiogenesis of adipose tissue via the α7nAChR-Egr1-FGF2 signaling pathway, and this angiogenesis mechanism was associated with increased adipogenesis in adipose tissue of female offspring.


Assuntos
Adipócitos/efeitos dos fármacos , Tecido Adiposo Branco/irrigação sanguínea , Neovascularização Fisiológica/efeitos dos fármacos , Nicotina/toxicidade , Agonistas Nicotínicos/toxicidade , Efeitos Tardios da Exposição Pré-Natal , Células 3T3-L1 , Adipócitos/metabolismo , Animais , Proteína 1 de Resposta de Crescimento Precoce/genética , Proteína 1 de Resposta de Crescimento Precoce/metabolismo , Feminino , Fator 2 de Crescimento de Fibroblastos/genética , Fator 2 de Crescimento de Fibroblastos/metabolismo , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Metabolismo dos Lipídeos/efeitos dos fármacos , Exposição Materna , Camundongos , Gravidez , Ratos Wistar , Transdução de Sinais/efeitos dos fármacos , Receptor Nicotínico de Acetilcolina alfa7/genética , Receptor Nicotínico de Acetilcolina alfa7/metabolismo
4.
J Cell Physiol ; 235(2): 1197-1208, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31270811

RESUMO

Chemotherapy is the first-line treatment option for patients with lung cancer. However, therapeutic resistance occurs through an incompletely understood mechanism. Our research wants to investigate the influence of Caveolin-1 (Cav-1) on the therapeutic sensitivity of lung cancer in vitro. Results in this study demonstrated that Cav-1 levels were markedly inhibited in A549 lung cancer cells after exposure to cisplatin. Knockdown of caveolin further enhanced cisplatin-triggered cancer death in A549 cells. The functional investigation demonstrated that Cav-1 inhibition amplified the mitochondrial stress signaling induced by cisplatin, as evidenced by the mitochondrial reactive oxygen species burst, cellular metabolic disruption, mitochondrial membrane potential reduction, and mitochondrial caspase-9-related apoptosis activation. At the molecular level, cav-1 augmented cisplatin-mediated mitochondrial damage by inhibiting Parkin-related mitochondrial autophagy. Mitophagy activation effectively attenuated the promotive impact of Cav-1 knockdown on mitochondrial damage and cell death. Furthermore, our data indicated that Cav-1 affected Parkin-related mitophagy by activating the Rho-associated coiled-coil kinase 1 (ROCK1) pathway; inhibition of the ROCK1 axis prevented cav-1 knockdown-mediated cell death and mitochondrial damage. Taken together, our results provide ample data illuminate the necessary action exerted by Cav-1 on affecting cisplatin-related therapeutic resistance. Silencing of Cav-1 inhibited Parkin-related mitophagy, thus amplifying cisplatin-mediated mitochondrial apoptotic signaling. This finding identifies the Cav-1/ROCK1/Parkin/mitophagy axis as a potential target to overcome cisplatin-related resistance in lung cancer cells.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31629777

RESUMO

Methamphetamine (METH) is one of the most prevalently used illegal psychostimulants in many countries. Continuous exposure to METH leads to behavioral sensitization in animals, which can be used as a behavioral model with many mechanisms in common with relapse in humans. Molecular hydrogen has recently gained attention for its potential as a novel healthcare product with preventive and therapeutic applicability to a wide range of pathological conditions. However, it remains unclear whether and, if so, how hydrogen regulates METH-induced behavioral abnormalities. In the present study, we investigated the roles of molecular hydrogen on the acquisition and transfer of METH-induced behavioral sensitization and the accompanying changes in ERK phosphorylation and ΔFosB activation in the nucleus accumbens (NAc) of mice. To this end, male C57BL/6 mice received METH (0.1, 0.5 and 1.0 mg/kg, i.p.) injections for 7 days followed by a METH challenge (0.1, 0.5 and 1.0 mg/kg, i.p.) after a 7-day transfer period. Molecular hydrogen, delivered through a hydrogen-rich saline (HRS) injection (10 mL/kg, i.p., 3-h interval), was administered during the acquisition and transfer periods. We found that HRS administration was able to inhibit the acquisition and transfer of 0.1 and 0.5 mg/kg METH-induced behavioral sensitization to a certain extent, thereby attenuating the expression of behavioral sensitization. The HRS injections alone did not induce any obvious changes in locomotor activity in mice. Intriguingly, the increases in pERK and ΔFosB in the NAc, which accompanied the METH-induced behavioral sensitization, were also attenuated by the HRS treatments. Due to the anti-oxidative function of molecular hydrogen, the HRS injections reduced METH-induced reactive oxygen species and malondialdehyde generation in the NAc. These results suggest that molecular hydrogen serves as an anti-oxidative agent with potentially therapeutic applicability to the treatment of METH addicts.

6.
J Hazard Mater ; 383: 121166, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31520932

RESUMO

The development of visible-light-responsive photocatalysts with organic semiconductors is considered as an important and promising way to solve the energy crisis and environmental pollution. In this work, PTCDI and PTCDI-PANI(Fe(III)-doped) heterostructure were prepared through radical polymerization and modified amidation reaction. They could be used as visible-light-driven photocatalysts for the treatment of medical wastewater containing Staphylococcus aureus and E. coli bacteria. The as-prepared PTCDI-PANI(Fe(III)-doped) exhibited comparably higher photocatalytic activity beyond PTCDI. Under the irradiation of visible light, the PTCDI-PANI(Fe(III)-doped) showed optimized sterilization efficiencies towards E. coli and Staphylococcus aureus bacteria, which was 95.8% and 99.3%, respectively. The enhanced photo-induced antibacterial effect by PANI(Fe(III)-doped)-grafting was mainly attributed to: (1) PANI has a narrow band gap of 2.8 eV; (2) The relatively higher conduction band position (-2.1 eV) of PANI promotes the reaction of O2+e-⟶·OH; (3) The strengthened junctions of amide bond between PTCDI and PANI improve the separation efficiency of photogenerated electron-hole pairs; (4) The relatively rougher surface of PTCDI-PANI comparing with PTCDI improves the bio-surface interactions or bacterial adhesion; (5) Fe(III)-doping acts as a conducting tunnel and promotes the charge transfer between PANI and PTCDI.

8.
Stem Cell Res ; 41: 101628, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31678776

RESUMO

CUL4B gene mutation can cause intelligence deficiency 15, a syndromic form of X-linked mental retardation characterized by severe intellectual deficit associated with short stature, craniofacial dysmorphism, speech delay and impairment, tremor and gait ataxia. Here, we generated iPSCs from a Chinese patient with c.1007_1011del (p.(Ile336fs)) in CUL4B gene by reprogramming peripheral blood mononuclear cells with non-integrating vectors. The generated iPSC line (SDQLCHi015-A) expresses pluripotency markers, presents a normal karyotype and is able to differentiate into three germ layers.

9.
Int J Pediatr Otorhinolaryngol ; 129: 109757, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31704576

RESUMO

INTRODUCTION: Auditory Neuropathy Spectrum Disorder (ANSD) is characterized by hearing loss ranging from normal to profound. Additionally, results are confounded by commonly fluctuating hearing thresholds in ANSD. As such, we sought to evaluate results of audiometric testing on children with ANSD and the impact of age and time on testing results. METHODS: Retrospective chart review on children <18 years of age diagnosed with ANSD at two tertiary care academic institutions. Data analyzed included initial audiogram with speech detection thresholds (SDT) and pure tone averages (PTA) as well as most recent unaided audiogram SDT and PTA. RESULTS: 75 ANSD patients were analyzed, of which 32 (42.7%) were female. Bilateral and unilateral ANSD was seen in 55 (73.3%) and 20 (26.7%), respectively. A total of 130 ears with ANSD were assessed with 80 (61.5%) meeting inclusion criteria. Of these patients, the median age in years at first audiogram and most recent audiogram were 1.94 (0.45-13.68) and 4.22 (0.97-14.61), respectively. The median ages at which an SDT and PTA could first be acquired on the audiogram in ANSD patients were 1.94 (0.50-13.68) and 2.86 (0.45-13.68), respectively. The average SDT/PTA at the initial and most recent audiogram were 47.5/45.7 and 49.4/53.0 dB, respectively. SDT to PTA within the same audiogram exhibited strong correlation (r = 0.82, p < 0.001). Similarly, comparison of initial SDT to SDT at a later time interval showed strong correlation (r = 0.73, p < 0.001). SDT and PTA at initial audiogram and PTA at later time demonstrated lower correlation but was still statistically significant (r = 0.49, p < 0.009 and r = 0.51, p < 0.044, respectively). Individual PTA was associated with age (r = -0.56, p < 0.001). CONCLUSIONS: SDT and PTA within the same audiogram and initial SDT to SDT acquired at a later time correlate strongly in audiometric testing in children with ANSD. Although not as strong, initial SDT and PTA still correlate with PTA at a later time interval. These findings suggest that audiometric results yielding a reliable SDT and frequency specific information necessary to calculate the PTA is not typically obtained until 2-3 years old. However, once this information is obtained, the child's hearing is fairly stable but may fluctuate over time.

10.
Stem Cell Res ; 41: 101611, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31707209

RESUMO

We established an induced pluripotent stem cell (iPSC) line (SDQLCHi010-A) from peripheral blood mononuclear cells isolated from a 4-year-old boy with optic nerve malformation and intellectual disability carrying a heterozygous mutation (c.220A>G (p.S74G)) in PAX6 gene. Non-integrating episomal vectors containing OCT4, SOX2, KLF4, BCL-XL and MYC were used for reprogramming. The established iPSC line showed normal karyotype, expressed pluripotency markers, exhibited differentiation potential in vitro and kept PAX6 gene mutation.

11.
Stem Cell Res ; 41: 101635, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31707215

RESUMO

Autism spectrum disorder (ASD) is a childhood-onset neurodevelopmental disorder challenged in social reciprocity and restrictive repetitive behaviors. Here, we generated an induced pluripotent stem cell (iPSC) line SDQLCHi014-A from a patient with ASD and hyperactivity, carrying a 303 kb de novo deletion at chr3p26.1 implicating GRM7 gene by reprogramming urine cells with non-integrating vectors. SDQLCHi014-A have shown full pluripotency, differentiation capacity and genetic stability. This iPSC line provides a valuable resource to study the molecular mechanisms underlying ASD.

12.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 35(9): 832-837, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31750827

RESUMO

Objective To investigate the relationship between long-chain intergenic non-coding RNA324 (LINC00324) and immunophenotype in peripheral blood leukocytes of acute myeloid leukemia (AML) patients. Methods Real-time quantitative PCR and bioinformatics databases were utilized to analyze the expression level of LINC00324 in peripheral blood leukocytes and cell lines KG-1, THP-1 and U937 in AML patients. The relationships of the expression level of LINC00324 with the red blood cell and platelet count, the expression levels of LINC00324 and immunophenotypes in 40 AML patients were analyzed by Person correlation analysis. The immunophenotypes included CD14, CD68, CD64, CD11b, CD4, CD45, CD33, HLA-DR, CD163, CD2, CD58, CD117, CD43, CD34, CD99, CD8, CD38, CD10, CD13, CD56, CD7, TdT, CD235a, CD138, CD61, MPO and CD19. Simultaneously, the cBioPortal database datasets (TCGA, NEJM 2013) were used to analyze the clinical characteristics of 173 AML patients, and to analyze the correlations between the expression level of LINC00324 and the peripheral blood blast percentage and white blood cell count in tumor samples. Results The expression of LINC00324 in peripheral blood leukocytes of AML patients was down-regulated, and its expression level was significantly correlated with immunophenotype CD33, red blood cell and platelet count. Analysis of bioinformatics database showed that LINC00324 was under-expressed in myeloid leukemia cell lines. The expression of LINC00324 in AML patients was associated with multiple immunophenotypes such as CD33, CD117, CD11b, CD14 and CD64 and was negatively correlated with peripheral blood blast percentage and white blood cell count. Conclusion LINC00324 may be involved in regulating the differentiation, development and function of immune cells, which providing a new strategy for the development of targeted drugs or treatment of AML.


Assuntos
Imunofenotipagem , Leucemia Mieloide Aguda/classificação , RNA Longo não Codificante/genética , Linhagem Celular Tumoral , Antígenos HLA-DR , Humanos , Contagem de Leucócitos , Leucócitos/metabolismo
13.
Sensors (Basel) ; 19(23)2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31771175

RESUMO

Single-pixel compressive imaging can recover images from fewer measurements, offering many benefits especially for the imaging modalities where array detection is unavailable. However, the widely used random projections fail to explore internal relations between coding patterns and image reconstruction. Here, we propose a single-pixel imaging method based on a deterministic origami pattern construction that can lead to a more accurate pattern ordering sequence and better imaging quality. It can decrease the sampling ratio, closer to the upper bounds. The experimental realization of this approach is a big step forward towards practical applications.

14.
Oxid Med Cell Longev ; 2019: 8218650, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31772710

RESUMO

Background/Aims: Polycystic ovary syndrome (PCOS) is an endocrine disorder characterized by abnormal hormone levels in peripheral blood and poor-quality oocytes. PCOS is a pathophysiological syndrome caused by chronic inflammation and oxidative stress. The aim of this study was to investigate the mechanism of melatonin regulation on androgen production and antioxidative damage in granulosa cells from PCOS patients with hypoestrogenia and hyperandrogenia. Methods: Cumulus-oocyte complexes were collected from PCOS patients who had low levels of estrogen in follicular fluids. Results: Melatonin triggered upregulation of cytochrome P450 family 19 subfamily A member 1 (CYP19A1) expression via the extracellular signal-regulated kinase pathway in luteinized granulosa cells. As a result, conversion of androgen to 17ß-estradiol was accelerated. We also found that melatonin significantly reduced the levels of inducible nitric oxide (NO) synthetase and NO in luteinized granulosa cells. Levels of transcripts encoding NF-E2-related factor-2 and its downstream target heme oxygenase-1 were also increased, leading to anti-inflammatory and antioxidant effects. We also found that melatonin could improve oocyte development potential. Conclusion: Our preliminary results showed that melatonin had a positive impact on oocyte quality in PCOS patients with hypoestrogenia and hyperandrogenia.

15.
Mikrochim Acta ; 186(12): 851, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31776683

RESUMO

A rapid, sensitive, and selective fluorometric assay is described for the determination of chromium(VI) in real waters and living cells. The method is making use of nitrogen, phosphorus, and sulfur tri-doped carbon dots (NPS-CDs) which have absorption/emission maxima at 360/505 nm/nm. Cr(VI) has an absorption maximum at 350 nm and causes an inner filter effect (IFE) on the blue fluorescence of the NPS-CDs. The NPS-CDs were hydrothermally synthesized using p-aminobenzenesulfonic acid and tetrakis(hydroxymethyl)phosphonium chloride as precursors. The NPS-CDs were characterized by transmission electron microscopy, X-ray diffraction, and several spectroscopic methods. They are biocompatible and negligibly cytotoxic when tested with HeLa cells and MCF-7 cells even after 48 h of incubation. The NPS-CDs were used as fluorescent probes for Cr(VI). The detection limit is 0.23 µM (three times standard deviation versus slope), and the linear response covers the 1 to 500 µM chromate concentration range. The NPS-CDs were applied to the determination of Cr(VI) in real waters and living cells (HeLa and MCF-7) and gave satisfying results. Graphical abstractSchematic representation of hydrothermal synthesis of nitrogen, phosphorus, and sulfur tri-doped carbon dots (NPS-CDs) for Cr(VI) detection via inner filter effect (IFE). NPS-CDs were applied to the determination of Cr(VI) in living cells (HeLa and MCF-7) with satisfying results.

16.
J Org Chem ; 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31777249

RESUMO

An efficient approach to access functionalized tertiary type ß-hydroxyl carboxamides has been developed through Sc(OTf)3-catalyzed addition of ynamides and substituted ketones. Water was found to be an important reaction substrate and solvent is no needed in this process. A broad range of substituted ynamides and ketones were well applicable to the reaction with excellent chemical selectivities. Moreover, several chiral ß-hydroxyl carboxamides 3j-3r were prepared with excellent regioselectivities and outstanding diastereoselectivities.

17.
Macromol Rapid Commun ; : e1900547, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31778285

RESUMO

The functionalization and application of nano-objects generated using a polymerization induced self-assembly (PISA) procedure is becoming a focus in recent years. In this contribution, using ethanol as solvent, poly(oligo(ethylene oxide) methyl ether methacrylate) (POEOMA) as macro-initiator/stabilizer, and 2-(perfluorohexyl)ethyl methacrylate (PFHEMA) and glycidyl methacrylate (GMA) as comonomers, the initiators for continuous activator regeneration atom transfer radical polymerization (ICAR ATRP)-based PISA is realized. The lower GMA content system tends to form spheres with large diameters and heavy contrast, while the lower PFHEMA content system tends to form the spheres or short worms with small diameters. However, the system with further increased GMA content results in the failed ICAR ATRP PISA procedure with the formation of precipitates by the cross-linking reaction between pendant epoxy groups. Furthermore, using the efficient reaction between the epoxy group on GMA and thiol group on mercapto-succinic acid agent, the carboxyl groups can be introduced into the inner cavity of the nano-objects and used for incorporation with the Fe2+ and Fe3+ ions, and the organic-inorganic nanoparticles Fe3 O4 @POEOMA are finally prepared in the presence of a reductant.

18.
Sci Total Environ ; 703: 134897, 2019 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-31731157

RESUMO

Submarine groundwater discharge (SGD)-driven nutrient inputs have long been speculated to sustain the high frequency of red tide occurrence in Tolo Harbour, Hong Kong, for its larger flux and higher nutrient loadings than river discharge. Based on analysis of high resolution time series biogeochemical and climatological data from 2000 to 2015, fresh SGD-derived dissolved inorganic nitrogen (DIN) is found to be a significant regulator of the annual cycle of phytoplankton community structure in the harbour. In the wet season, fresh SGD supplies nutrients with NH4+:NO3- ratio < 1 to the seawater, meanwhile creates an intensive vertical stratification environment. As a result, diatom which is a NO3- specialist, is prone to be the major group in the harbour. Fresh SGD delivers a same orders of magnitude of DIN as river and precipitation, but it is more important to phytoplankton community structure dynamics because fresh groundwater has smaller NH4+:NO3- ratio that significantly changes the ratio in the harbour. In the dry season, with the decline of fresh SGD and the ease of stratification, vertical mixing uplifts the nutrient (NH4+:NO3- ratio > 1) released from the bottom sediment leading to a NH4+ dominant environment in water column. Dinoflagellate and other groups then become dominant species of phytoplankton in the harbour. Fresh SGD has a major influence on the NH4+:NO3- ratio in the seawater compared to tide-driven SGD, even though the latter contributes a larger proportion SGD. Tide-driven SGD also produces NH4+ and NO3-, but NH4+:NO3- ratio are mainly subject to the beach environment (bare/mangrove beach), which does not change much seasonally, thus dominant DIN species do not change significantly throughout a year. In a conclusion, fresh SGD plays the most important role among all the endmembers in regulating the DIN composition in Tolo Harbour and its fluctuation mediates the phytoplankton community structure.

19.
Stem Cell Res ; 41: 101585, 2019 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-31775088

RESUMO

The human induced pluripotent stem cell (iPSC) line SDQLCHi013-A was generated from peripheral blood mononuclear cells of a 7-day-old infant, who was diagnosed with maple syrup urine disease and carried compound heterozygote mutations (c.1280_1282 delTGG and c.632C>T) in BCKDHA gene. Non-integrating episomal vectors coding OCT4, SOX2, KLF4, BCL-XL and MYC were used for reprogramming. The established iPSC line contained the same mutations found in the patient, possessed a normal karyotype, could differentiate into cells of three germ layers in vitro and expressed pluripotency markers.

20.
Chem Commun (Camb) ; 55(94): 14170-14173, 2019 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-31701971

RESUMO

A zinc-catalyzed decarboxylative A3 reaction of cyclic amino acids, α,ß-unsaturated aldehydes and terminal alkynes has been developed. A series of functionalized N-heterocyclic 1,6-enynes have been successfully obtained with excellent regioselectivities through this novel approach. In addition, the utility of this straightforward process is demonstrated by the preparation of a polycyclic nitrogen-containing heterocyclic compound.

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