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1.
Gene ; 747: 144671, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32304782

RESUMO

In diploid organisms, each gene locus is composed of two parental alleles, which would interact with each other for determining the phenotypic variation. Better understanding of the allele-specific expression (ASE) in farm animals is much important to explore the genetic basis underlying economically important traits, which have been poorly understood yet. In this study, genome-wide analysis was applied to explore the spatiotemporal pattern of ASE in the F1 hybrids of chicken. First, meat- and egg-type chickens were selected for producing a full-sib F1 hybrid population (n = 57). Then, genome resequencing of two parents and 38 offspring were performed and liver and breast muscle samples (n = 38) were subjected to strand-specific RNA sequencing (ssRNA-seq) for ASE detection at 1, 28, and 56 days of age, respectively. The results accurately identified a total of 465 informative genes that could be distinguished with respect to their parental origins. There were 0.4% - 4.1% of informative genes showing ASE, and 57 of them were found across different tissues and time points. Besides, most ASE genes in chickens were tissue-specific, and no matter what the time-point pattern of one ASE gene, the same parental allele of this gene almost showed consistently higher or lower expression across all time points in the same type tissue. In conclusion, this study indicated that most of ASE genes were tissue-specific and time-dependent.


Assuntos
Alelos , Galinhas/genética , Cruzamentos Genéticos , Ovos , Regulação da Expressão Gênica , Genoma , Hibridização Genética , Carne , Animais , Feminino , Masculino , Especificidade de Órgãos/genética , Polimorfismo de Nucleotídeo Único/genética , Fatores de Tempo
2.
BMC Genet ; 21(1): 12, 2020 02 04.
Artigo em Inglês | MEDLINE | ID: mdl-32019486

RESUMO

BACKGROUND: Modern breeding in the poultry industry mainly aims to produce high-performance poultry lines and breeds in two main directions of productivity, meat and eggs. To understand more about the productive potential of lowly selected Chinese native chicken populations, we selected 14 representative SNP markers strongly associated with growth traits or carcass traits and 14 SNP markers strongly associated with egg laying traits through previous reports. By using the MassArray technology, we detected the genotype frequency distributions of these 28 SNP markers in seven populations including four lowly selected as well as one moderately selected Sichuan native chicken populations, one commercial broiler line and one commercial layer line. RESULTS: Based on the genotype frequency distributions of these 28 SNP markers in 5 native chicken populations and 2 commercial lines, the results suggested that these Chinese indigenous chicken populations have a relatively close relationship with the commercial broiler line but a marked distinction from the commercial layer line. Two native chicken breeds, Shimian Caoke Chicken and Daheng Broilers, share similar genetic structure with the broiler line. CONCLUSIONS: Our observations may help us to better select and breed superior domestic chickens and provide new clues for further study of breeding programs in local chicken populations.

3.
Sci Rep ; 10(1): 2421, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32051476

RESUMO

This study evaluates the use of L-PRF as an autologous scaffold in nerve regeneration, and Schwann cells (SCs) proliferation and secretion of neurotrophic factors and its anti-inflammatory effect on SC Porphyromonas Gingivalis-Lipopolysaccharide (PG-LPS)-induced inflammatory responses in vitro. SEM was done to investigate various features of L-PRF. L-PRF-extracts was used to investigate the release of growth factors and treatment of SCs line. ELISA was applied to examine the release of IGF-1. The proliferative effect of L-PRF on SCs was assessed with CCK-8 assay. The effect of L-PRF on the mRNA and protein expression of SC neurotrophic factors were analyzed by RT-qPCR and ELISA. CCK-8 assay and RT-qPCR were used to determine the required concentration and the action time of PG-LPS before the anti-inflammatory effect of L-PRF was determined by measuring the changes in IL-1ß, IL-6, and TNF-a with RT-qPCR and ELISA. There are different features in L-PRF. Fourteen days was sufficient to release adequate GF. The mRNA expressions of the pro-inflammatory cytokines were notably raised by PG-LPS in 3-hours treatment. L-PRF can increase SC proliferation, neurotrophic factors secretion, and suppress SC PG-LPS-induced inflammatory responses in vitro. L-PRF has the potential as an autologous biological additive for peripheral nerve regeneration in the event of nerve inflammation and injuries.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32078788

RESUMO

Polymorphism at the 17q21 gene locus and wheezing responses to rhinovirus (RV) early in childhood conspire to increase the risk of developing asthma. However, the mechanisms mediating this gene-environment interaction remain unclear. In this study, we investigated the impact of one of the 17q21 encoded genes, ORMDL3 on RV replication in human epithelial cells. ORMDL3 knockdown inhibited RV-A16 replication in HeLa, BEAS-2B, A549, NCI-H358 epithelial cell lines, and primary nasal and bronchial epithelial cells. Inhibition varied by RV species, as both minor and major group RV-A subtypes, RV-B52 and RV-C2 were inhibited, but not RV-C15 or RV-C41. ORMDL3 siRNA did not affect expression of the major group RV-A receptor ICAM-1 or initial internalization of RV-A16. The two major outcomes of ORMDL3 activity, serine palmitoyl-CoA transferase (SPT) inhibition and endoplasmic reticulum (ER) stress induction were further examined: silencing ORMDL3 decreased RV-induced ER stress and IFN- mRNA expression. However, pharmacologic induction of ER stress and concomitant increased IFN- inhibited RV-A16 replication. Conversely, blockade of ER stress with tauroursodeoxycholic acid augmented replication, pointing to an alternative mechanism for the effect of ORMDL3 knockdown on RV replication. In comparison, the SPT inhibitor myriocin increased RV-A16 but not RV-C15 replication, and negated the inhibitory effect of ORMDL3 knockdown. Further, lipidomics analysis revealed opposing regulation of specific sphingolipid species (downstream of SPT) by myriocin and ORMDL3 siRNA, correlating with the effect of these treatments on RV replication. Together this data revealed a requirement for ORMDL3 in supporting RV replication in epithelial cells via SPT inhibition.

5.
Talanta ; 211: 120712, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32070589

RESUMO

Online measuring end-tidal propofol concentration during balanced anesthesia is important for anesthetists to learn the patient's anesthesia depth as exhaled propofol concentration is well related to blood propofol concentration. In previous work, exhaled propofol was detected using acetone assisted negative photoionization ion mobility spectrometer, however, the existence of high concentration sevoflurane interfered the response of propofol. In this work, an anisole assisted photoionization ion mobility spectrometer operated in positive mode was developed to sensitively and selectively measure the end-tidal propofol by eliminating the interferences of exhaled humidity and sevoflurane during balanced anesthesia. Anisole molecular ion is stable enough not to go under proton transfer reaction with water presents in the exhaled breath. Hence, the exhaled humidity related peaks were eliminated and only one propofol product ion peak (K0 = 1.50 cm2 V-1 s-1) was observed. The relative standard deviation (RSD) ranging from 0.64%-0.91% showed good repeatability and the quantitative range was 0.2-40 ppbv with a response time of 4 s. Finally, the performance of the proposed method was demonstrated by monitoring end-tidal propofol of balanced anesthetized patients during gastric cancer surgery.

6.
PLoS One ; 15(1): e0227794, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31999761

RESUMO

Peony is a famous ornamental and medicinal plant in China, and peony hybrid breeding is an important means of germplasm innovation. However, research on the genome of this species is limited, thereby hindering the genetic and breeding research on peony. In the present study, simple sequence repeat (SSR) locus analysis was performed on expressed sequence tags obtained by the transcriptome sequencing of Paeonia using Microsatellite software. Primers with polymorphism were obtained via polymerase chain reaction amplification and electrophoresis. As a result, a total of 86,195 unigenes were obtained by assembling the transcriptome data of Paeonia. Functional annotations were obtained in seven functional databases including 49,172 (Non-Redundant Protein Sequence Database: 57.05%), 38,352 (Nucleotide Sequence Database: 44.49%), 36,477 (Swiss Prot: 42.32%), 38,905 (Clusters of Orthologous Groups for Eukaryotic Complete Genomes: 45.14%), 37,993 (Kyoto Encyclopedia of Genes and Genomes: 44.08%), 26,832 (Gene Ontology: 31.13%) and 37,758 (Pfam: 43.81%) unigenes. Meanwhile, 21,998 SSR loci were distributed in 17,567 unigenes containing SSR sequences, and the SSR distribution frequency was 25.52%, with an average of one SSR sequence per 4.66 kb. Mononucleotide, dinucleotide, and trinucleotide were the main repeat types, accounting for 55.74%, 25.58%, and 13.21% of the total repeat times, respectively. Forty-five pairs of the 100 pairs of primers selected randomly could amplify clear polymorphic bands. The polymorphic primers of these 45 pairs were used to cluster and analyze 16 species of peony. The new SSR molecular markers can be useful for the study of genetic diversity and marker-assisted breeding of peony.


Assuntos
Repetições de Microssatélites , Paeonia/genética , Transcriptoma , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Genes de Plantas , Genômica , Melhoramento Vegetal , Polimorfismo Genético
7.
Evol Comput ; 28(1): 1-25, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-30475673

RESUMO

Pareto-based multi-objective evolutionary algorithms experience grand challenges in solving many-objective optimization problems due to their inability to maintain both convergence and diversity in a high-dimensional objective space. Exiting approaches usually modify the selection criteria to overcome this issue. Different from them, we propose a novel meta-objective (MeO) approach that transforms the many-objective optimization problems in which the new optimization problems become easier to solve by the Pareto-based algorithms. MeO converts a given many-objective optimization problem into a new one, which has the same Pareto optimal solutions and the number of objectives with the original one. Each meta-objective in the new problem consists of two components which measure the convergence and diversity performances of a solution, respectively. Since MeO only converts the problem formulation, it can be readily incorporated within any multi-objective evolutionary algorithms, including those non-Pareto-based ones. Particularly, it can boost the Pareto-based algorithms' ability to solve many-objective optimization problems. Due to separately evaluating the convergence and diversity performances of a solution, the traditional density-based selection criteria, for example, crowding distance, will no longer mistake a solution with poor convergence performance for a solution with low density value. By penalizing a solution in term of its convergence performance in the meta-objective space, the Pareto dominance becomes much more effective for a many-objective optimization problem. Comparative study validates the competitive performance of the proposed meta-objective approach in solving many-objective optimization problems.

8.
Biomed Res Int ; 2019: 3295756, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31886202

RESUMO

Platelet-rich fibrin (PRF) is an autologous platelet concentrate that consists of cytokines, platelets, leukocytes, and circulating stem cells. It has been considered to be effective in bone regeneration and is mainly used for oral and maxillofacial bone. Although currently the use of PRF is thought to support alveolar ridge preservation, there is a lack of evidence regarding the application of PRF in osteogenesis. In this paper, we will provide examples of PRF application, and we will also summarize different measures to improve the properties of PRF for achieving better osteogenesis. The effect of PRF as a bone graft material on osteogenesis based on laboratory investigations, animal tests, and clinical evaluations is first reviewed here. In vitro, PRF was able to stimulate cell proliferation, differentiation, migration, mineralization, and osteogenesis-related gene expression. Preclinical and clinical trials suggested that PRF alone may have a limited effect. To enlighten researchers, modified PRF graft materials are further reviewed, including PRF combined with other bone graft materials, PRF combined with drugs, and a new-type PRF. Finally, we will summarize the common shortcomings in the application of PRF that probably lead to application failure. Future scientists should avoid or solve these problems to achieve better regeneration.


Assuntos
Processo Alveolar , Regeneração Óssea/efeitos dos fármacos , Transplante Ósseo , Procedimentos Cirúrgicos Bucais , Osteogênese/efeitos dos fármacos , Fibrina Rica em Plaquetas , Processo Alveolar/metabolismo , Processo Alveolar/patologia , Processo Alveolar/cirurgia , Animais , Transplante Ósseo/classificação , Transplante Ósseo/métodos , Humanos , Procedimentos Cirúrgicos Bucais/classificação , Procedimentos Cirúrgicos Bucais/métodos
9.
Materials (Basel) ; 12(23)2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31779071

RESUMO

Incorporation of nanoparticles into polymer matrix was found to considerably improve the compressive performance of unidirectional fiber reinforced composites. In our experimental study, an increase by 62.7% in the longitudinal compressive strength of unidirectional carbon fiber reinforced composites is attained by dispersing 8.7 vol.% SiO2 nanoparticles into epoxy matrix. A compressive strength model is established to quantitatively describe the reinforcing effects of nanoparticles, which combines a modified microbuckling model for unidirectional fiber reinforced composites and a constitutive model for nanocomposite matrices under compression. In the two models, the coupling of damage and plasticity is considered to contribute to the nonlinear response of nanocomposite matrix. The proposed strength model demonstrates excellent prediction capability in experimental verification. A small relative deviation below 8.2% is achieved between the predicted compressive strength of unidirectional fiber reinforced composites and the measured values, which is at the same level of random error in experiments.

10.
Int J Biol Macromol ; 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31751713

RESUMO

Hyaluronic acid (HA) exists naturally as an important component of the extracellular matrix (ECM) in the human body. In recent decades, HA has been widely used in bone regeneration, and is currently a popular topic, particularly in the craniofacial and dental fields. From maxilla augmentation to craniofacial bone trauma, there is now a large demand for bone regenerative therapy. Serving as a cell-seeding scaffold or a carrier for bioactive components, hyaluronic acid-incorporated scaffolds and carriers in bone regeneration can be fabricated into either rigid or colloidal forms. Since the type of material used is a critical factor in the biological properties of a scaffold, HA derivatives or HA-incorporated composite scaffolds have shown excellent potential for improving osteogenesis and mineralization. Furthermore, in order to better enhance osteogenesis, local delivery carriers based on hyaluronic acid derivatives, rather than specifically serving as scaffolds, can be established by loading different osteoinductive or osteogenetic components and acquiring different release patterns. Such osteoinductive carriers immobilized on implant surfaces are also effective in improving osseointegration. Thus, as such a competent biomaterial, hyaluronic acid should be considered a promising tool in bone regeneration.

12.
Environ Technol ; : 1-8, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31638475

RESUMO

To reduce the cost of polyhydroxyalkanoate (PHA) production and dispose the amount of waste sludge simultaneously, chemical oxygen demand (COD) concentration, pH and operation cycle were investigated to find the optimal PHA synthesis conditions with waste sludge in this study. The maximum PHA content (31.3% of the cell dry weight (CDW)), as well as the highest PHA conversion rate (0.30 mg COD/mg COD) and PHA-specific synthesis rate (6.12 mg COD/mg CDW·h), was achieved with initial COD concentration, pH value and operation cycle: 6000 mg/L, 8.5 and 24 h. In order to further investigate the process of PHA synthesis, COD removal rate and CDW were also introduced. This study could provide valuable information for increasing the production of PHA with waste sludge.

13.
Materials (Basel) ; 12(21)2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31652706

RESUMO

Epoxy polymer concrete (EPC) has found increasing applications in infrastructure as a rising candidate among civil engineering materials. In most of its service environments, EPC is inevitably exposed to severe weather conditions, e.g., violent changes in temperature, rain, and ultraviolet (UV) radiation. In this paper, we designed an accelerated aging test for EPC, which includes periodic variation of temperature and water spray, as well as intensive UV-light irradiation, imitating the outdoor environment in South China. The experimental results show that the flexural performance of EPC is found deteriorate with the aging time. An aging process equivalent to four years (UV radiation dose) results in up to 8.4% reduction of flexural strength. To explore the mechanisms of observed performance degradation, the EPC specimen in the four-point-bending test is considered as a layered beam. The analysis indicates that the loss of flexural load-carrying capacity of an aged EPC beam is dominated by the reduction of mechanical properties of the surface layer. The mechanical properties of the surface layer are closely associated with the aging of epoxy mortar, which can be approximated as a reciprocal function of the aging time. By introducing damage to the surface layer into the layered beam, the proposed model demonstrates a good ability to predict the residual flexural strength of EPC during the aging process.

14.
Exp Eye Res ; 187: 107756, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31421136

RESUMO

Endoplasmic reticulum (ER) stress is recognized as a contributing factor to various ocular neurovascular pathologies including retinitis pigmentosa, glaucoma, and diabetic retinopathy (DR). ER stress in particular is implicated in the development of DR, which is significantly influenced by inflammation driven retinal vascular degeneration and dysfunction. Ultimately, loss of vision occurs if left untreated. However, the identity of the target cells and their temporal involvement in diabetes-mediated dysfunction need further investigation. Early diabetes-induced stress in photoreceptor cells is proposed as the driver of inflammatory mediated neurovascular changes during diabetes. Although tunicamycin induced ER stress results in photoreceptor loss, its consequences for retinal vascular degeneration and retinal ganglion (RGC) and pigment epithelium (RPE) cell loss remains unclear. Here we show intravitreal delivery of tunicamycin primarily induced ER stress in photoreceptor cells resulting in their loss by apoptosis. This was concomitant with induced expression of the unfolded protein response marker CHOP in these cells. We also demonstrated significant degeneration of retinal capillaries following the loss of photoreceptor cells with minimal impact on loss of RGC and RPE cells. However, activation of retinal microglial and Muller cells were noticeable. Thus, our data support the notion that ER stress mediated dysfunction and/or loss of photoreceptor cells in response to inflammation and oxidative stress could precede retinal vascular and neuronal dysfunction and degeneration.


Assuntos
Antibacterianos/farmacologia , Células Fotorreceptoras de Vertebrados/patologia , Degeneração Retiniana/patologia , Células Ganglionares da Retina/patologia , Epitélio Pigmentado da Retina/patologia , Vasos Retinianos/patologia , Tunicamicina/farmacologia , Animais , Atrofia , Capilares/patologia , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Marcação In Situ das Extremidades Cortadas , Injeções Intravítreas , Masculino , Camundongos , Camundongos Endogâmicos C57BL , N-Metilaspartato/farmacologia , Estresse Oxidativo , Células Fotorreceptoras de Vertebrados/metabolismo , Degeneração Retiniana/metabolismo , Células Ganglionares da Retina/metabolismo , Epitélio Pigmentado da Retina/metabolismo , Proteína da Zônula de Oclusão-1/metabolismo
15.
Onco Targets Ther ; 12: 6157-6163, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31447564

RESUMO

Background: Acute promyelocytic leukemia (APL) is commonly characterized by the fusion of retinoic acid receptor alpha (RARA) with promyelocytic leukemia (PML). Most APL patients acquire long-term survival after treatment with all-trans retinoic acid (ATRA) or arsenic agents-based chemotherapy. Case presentation: A rare case of APL was reported after IRF2BP2-RARA was detected in the relapsed process using next-generation RNA-sequencing analysis. In addition, the mutation of NRAS was also detected. ATRA and arsenic trioxide combined with daunorubicin were used during induction treatment. The patient acquired complete remission but relapsed in 12 months. The patient was resistant to all other chemotherapies and refused any further therapy. The literature review indicated that allogeneic hematopoietic stem cell transplantation might be a therapeutic method to treat APL with IRF2BP2-RARA fusion. Conclusion: Atypical APL should be considered even if the patients present with normal chromosomal karyotype and no classic PML-RARA fusions, but classical clinical features and bone marrow cell morphology. We reported a case of APL with IRF2BP2-RARA fusion was shown to harbor the NRAS mutation at relapse.

16.
Medicine (Baltimore) ; 98(28): e16382, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31305439

RESUMO

RATIONALE: Type I monoclonal cryoglobulinemia is usually associated with lymphoproliferative disorders, such as monoclonal gammopathy of undetermined significance (MGUS), myeloma, chronic lymphocytic leukemia (CLL) and lymphoplasmocytic lymphoma (LPL). Clinical symptoms related to Type I cryoglobulin (CG) isotype often include skin, neurological and renal manifestations. PATIENT CONCERNS: A 42-year-old woman who initially presented urticaria, palpable purpura in both her upper extremities and legs, eosinophilia and Raynaud phenomenon. Skin biopsy revealed eosinophil infiltration. Monoclonal immunoglobulin (Ig) G-κprotein was detected and CG was also positive. DIAGNOSES: The patient was finally diagnosed as MGUS related Type I CG. INTERVENTIONS: Cyclophosphamide-dexamethasone-thalidomide (CDT) therapy was initiated. OUTCOMES: The treatment relieved the skin symptoms efficiently. LESSONS: To our knowledge, this is a rare case of Type I cryoglobulinemic vasulitis with eosinophilia complicated by MGUS, and the effective treatment of cyclophosphamide combined with thalidomide and prednisone may provide a new therapeutic option for cryoglobulinemic vasulitis.


Assuntos
Crioglobulinemia/complicações , Eosinofilia/complicações , Gamopatia Monoclonal de Significância Indeterminada/complicações , Vasculite/complicações , Adulto , Crioglobulinemia/diagnóstico , Crioglobulinemia/tratamento farmacológico , Diagnóstico Diferencial , Eosinofilia/diagnóstico , Eosinofilia/tratamento farmacológico , Feminino , Humanos , Gamopatia Monoclonal de Significância Indeterminada/diagnóstico , Gamopatia Monoclonal de Significância Indeterminada/tratamento farmacológico , Vasculite/diagnóstico , Vasculite/tratamento farmacológico
17.
Am J Reprod Immunol ; 82(3): e13159, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31206849

RESUMO

PROBLEM: The oviduct plays an indispensable role in the formation of eggs, especially the magnum and uterus. The identification of oviduct development in different stages will help to target candidate genes and pathways in regulation of albumen and eggshell formation, as well as defense mechanism in oviduct and egg. METHODS: To identify the function differences and the molecular defense mechanism of the oviduct and egg, we performed transcriptome sequencing analysis of the magnum and uterus in 120-d-old and 300-d-old Lohmann layers, three birds in each group. RESULTS: With fold changes (log2 ratio) ≥ 2 and false discovery rate (FDR) < 0.01, RNA-Seq revealed 1,040 genes expressed differentially in the magnum and 595 genes in the uterus. By combining GO enrichment and KEGG pathway analysis, it served to show that gene activities of the magnum and uterus in prelaying chickens were more likely to concentrate on growth and development, and after egg-laying, they were mainly inclined to enhance the substances transmembrane transport and secretion activities. We further characterized 1579 new genes, while only 803 of them were functionally annotated. A complex mixture of proteins related to defense was measured in this study. A subset of avian ß-defensins (AvBDs) and ovodefensins (OvoDs), that is, AvBD12, AvBD11, AvBD10, OvoDA1, OvoDB1, OvoDA2, OvoDA3, and OvoDBß, was detected to express in the magnum of laying hens at high levels. CONCLUSION: Collectively, the identification and functional analysis of these differentially expressed genes (DEGs), as well as specific expression of avian defensins, may contribute to understand the development and defense mechanisms of oviduct and eggs.

18.
Sensors (Basel) ; 19(11)2019 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-31159466

RESUMO

Long-term structural health monitoring (SHM) has become an important tool to ensure the safety of infrastructures. However, determining methods to extract valuable information from large amounts of data from SHM systems for effective identification of damage still remains a major challenge. This paper provides a novel effective method for structural damage detection by introduction of space and time windows in the traditional principal component analysis (PCA) technique. Numerical results with a planar beam model demonstrate that, due to the presence of space and time windows, the proposed double-window PCA method (DWPCA) has a higher sensitivity for damage identification than the previous method moving PCA (MPCA), which combines only time windows with PCA. Further studies indicate that the developed approach, as compared to the MPCA method, has a higher resolution in localizing damage by space windows and also in quantitative evaluation of damage severity. Finally, a finite-element model of a practical bridge is used to prove that the proposed DWPCA method has greater sensitivity for damage detection than traditional methods and potential for applications in practical engineering.

19.
3 Biotech ; 9(5): 203, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31065503

RESUMO

Coat color genetics successfully adapted and applied to different animal species, which provides a good demonstration of the concept of comparative genetics. In this study, we sequenced 945 bp fragments of melanocortin 1 receptor (MC1R) gene, 421 bp fragments of exon 1 of tyrosinase (TYR) gene and 266 bp fragments of exon 3 of agouti signaling protein (ASIP) gene for 250 individuals with five plumage color patterns. We detected a total of three SNPs (T398A, T637C, and G920C) in MC1R and built six haplotypes (H1-H6) based on the three SNPs. H5 and H6 haplotypes were mainly concentrated in white and grey chicken. And diplotypes H2H3 occurred in white feather and black-speckle feather with the same frequency. Moreover, a total of three SNPs (C47G, T120C, and T172C) in TYR were found and built six haplotypes (P1-P6) based on the three SNPs. Among them, haplotype P2, P3 and P6 were not occurred in black chicken, the diplotypes P1P6 and P4P6 were only distributed in white, gray and black-speckled feather. We only detected one SNP (T168C) in ASIP gene and found that genotype TT was advantage genotype in the different plumage color groups of chickens. Collectively, our study suggested an association between plumage color and genetic variation of MC1R, TYR and ASIP in chicken.

20.
3 Biotech ; 9(6): 222, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31114746

RESUMO

KLF5 is an important regulator of cell proliferation, differentiation, and apoptosis in mammals. Little is known about the function of KLF5 in the regulation of chicken. Hence, qPCR was used to detect the expression of KLF5 in different tissues of chicken. And chicken skeletal muscle satellite cells (SMSCs) were transfected KLF5-specific small interfering RNA (siRNA) to assay SMSCs' proliferation, differentiation, and apoptosis. The results showed that KLF5 expressed higher in skeletal muscle than in the other tissues of chicken. Knockdown of KLF5 significantly inhibited the differentiation and increased apoptosis of chicken SMSCs, but it had no significant effect on proliferation of SMSCs. These results indicate that KLF5 plays an essential role during myogenesis, which will affect muscle repair and muscle regeneration, and may ameliorate muscle aging or sarcopenia.

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