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1.
Chin J Nat Med ; 18(4): 250-255, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32402400

RESUMO

Penicildiones A-D (1-4), four new steroids derivatives together with three known compounds including 16α-methylpregna-17α,19-dihydroxy-(9,11)-epoxy-4-ene-3,18-dione-20-acetoxy (5), stachybotrylactone B (6) and stachybotrin (7) were isolated from the soft coral-derived fungus Penicillium sp. SCSIO41201, cultured in the 1% NaCl PDB substrate. Their structures were determined through spectroscopic methods and X-ray crystallography. Biological evaluation results revealed that 6 exhibited significant cytotoxic activity against HL-60, K562, MOLT-4, ACHN, 786-O, and OS-RC-2 cell lines with IC50 values of 5.23, 4.12, 4.31, 23.55, 7.65 and 10.81 µmol·L-1, respectively, while other compounds showed weak or no cytotoxicity at 50 µmol·L-1.

2.
Sci Total Environ ; 730: 139119, 2020 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-32402973

RESUMO

Biochars are widely used in the remediation of soil heavy metals, but there has been no clear understanding to the effects of novel co-pyrolysis biochars derived from biomass and orthophosphate on soil heavy metals. In this study, co-pyrolysis biochars derived from rape straw and orthophosphate (Ca (H2PO4)2·H2O/KH2PO4) were prepared and used to explore their effects on the speciations and ecological risks of Pb, Cd, and Cu in contaminated agricultural soil. The results showed that the addition of these co-pyrolysis biochars significantly decreased TCLP extracted concentrations (decreased by 5.9-81.7%) and ecological risks of heavy metals (Pb, Cd, and Cu) by transforming the metals from available speciation to stable speciation in soils. Co-pyrolysis biochar derived from rape straw and KH2PO4 showed the highest immobilization capacities, and the immobilization capacities of biochars for three metals were in the order of Pb > Cu > Cd. Co-pyrolysis biochars could precipitate and complex with heavy metals directly by the phosphate and -OH on their surface, and also could promote immobilization of heavy metals indirectly by increasing soil pH value and available P content. During incubation, the content of carboxyl groups on biochars increased significantly, which was beneficial to the further complexation of heavy metals. In summary, the application of co-pyrolysis biochar derived from rape straw and orthophosphate (especially for KH2PO4) could effectively reduce ecological risks of Pb, Cd, and Cu in contaminated soil.

3.
Sci Total Environ ; 730: 138928, 2020 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-32388371

RESUMO

Heavy metal pollution has posed a potential hazard to the ecological environment and human health. Herein, a novel ternary magnetic adsorbent (Fe3O4/g-C3N4/Carbon layer, Carbon layer: hydrothermal products from sucrose) was synthesized through a simple hydrothermal carbonization (HTC) method for removal of hexavalent chromium (Cr (VI)) removal. The Carbon layer (CL) formed during the HTC of carbon precursors (sucrose) acted as a reducing agent. Also, it has abundant oxygen-containing groups on its surface. The Fe3O4/g-C3N4/CL had a high removal capacity for Cr (VI) (50.09 mg/g), and excellent regeneration and magnetic separation performance. Importantly, the Fe3O4/g-C3N4/CL could not only improve the adsorption ability for Cr (VI), but also strengthen the immobilization of Cr (III). Based on the comprehensive experiments and characterization, combined with DFT calculations, we proposed that, the first time, the removal of Cr (VI) was controlled by three consecutive processes: (1) ion exchange of Cr (VI) by hydroxyl groups, (2) reduction of Cr (VI) to Cr (III) by electron-donor (oxygen-containing) groups (EDGs), and (3) complexation of Cr (III) by amine groups. This study provides a new avenue for the removal of toxic oxygen anions and reveals an original removal mechanism of Fe3O4/g-C3N4/CLx (x = hydrothermal products from carbon precursors (glucose, ascorbic acid, cellulose)).

4.
J Nat Prod ; 83(4): 1258-1264, 2020 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-32283019

RESUMO

Seven unusual new ene-yne hydroquinones (1-3, 5-8), including three rare glycosylated derivatives named pestalotioquinosides A-C (6-8), were obtained from the marine-derived strain SCSIO41403 of the fungus Pestalotiopsis neglecta. Their structures including absolute configurations were elucidated by spectroscopic analysis and induced electronic circular dichroism experiments. In silico molecular docking and in vitro surface plasmon resonance studies showed that pestalotioquinoside C (8) could act as a liver X receptor alpha (LXRα) modulator. Further study showed that LXR target gene ABCA1 was significantly upregulated by 8, which revealed 8 as a potential LXRα agonist.

5.
Transbound Emerg Dis ; 2020 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-32348020

RESUMO

Human rabies is a public health problem in Asia, especially in less-developed regions where the disease is under-reported because of a lack of epidemiological surveillance. To address this gap, we collected data on human rabies in Yunnan Province, China, between 2005 and 2016. Using statistical mapping techniques, we correlated the occurrence of human rabies to environmental (elevation, precipitation, normalized difference vegetation index [NDVI], temperature and distance to the nearest main rivers) and anthropogenic (human and dog population density, distance to the nearest main roads and gross domestic product [GDP]) factors. We used a performance score, the average area under the receiver operator characteristic curve (0.88), to validate our risk model. Using this model, we found that environmental factors were more strongly associated with human rabies occurrence than anthropogenic factors. Areas with elevation below 2000 metres, GDP per capita between $750 and $4500/year and NDVI below 0.07 were associated with greater risk of human rabies. Rabies control in China should specifically target these areas.

6.
Science ; 368(6491): 638-642, 2020 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-32234804

RESUMO

Responding to an outbreak of a novel coronavirus [agent of coronavirus disease 2019 (COVID-19)] in December 2019, China banned travel to and from Wuhan city on 23 January 2020 and implemented a national emergency response. We investigated the spread and control of COVID-19 using a data set that included case reports, human movement, and public health interventions. The Wuhan shutdown was associated with the delayed arrival of COVID-19 in other cities by 2.91 days. Cities that implemented control measures preemptively reported fewer cases on average (13.0) in the first week of their outbreaks compared with cities that started control later (20.6). Suspending intracity public transport, closing entertainment venues, and banning public gatherings were associated with reductions in case incidence. The national emergency response appears to have delayed the growth and limited the size of the COVID-19 epidemic in China, averting hundreds of thousands of cases by 19 February (day 50).


Assuntos
Betacoronavirus , Infecções por Coronavirus/prevenção & controle , Infecções por Coronavirus/transmissão , Pandemias/prevenção & controle , Pneumonia Viral/prevenção & controle , Pneumonia Viral/transmissão , Viagem , China/epidemiologia , Controle de Doenças Transmissíveis , Infecções por Coronavirus/epidemiologia , Epidemias , Humanos , Incidência , Modelos Estatísticos , Pneumonia Viral/epidemiologia , Prática de Saúde Pública , Análise de Regressão
7.
J Antibiot (Tokyo) ; 2020 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-32286514

RESUMO

Three new carboxylic acid derivatives, pestallic acids F and G (1 and 2), pestalotiopyrone N (3), and a new diphenylketone derivative named neopestalone (5) were obtained from the liquid cultures of marine alga-derived endophytic fungus Pestalotiopsis neglecta SCSIO41403, along with six known compounds (4, 6-10). The structures of those new compounds were elucidated mainly by analysis of their NMR and MS data. The isolated compounds were evaluated for their anti-Dengue virus and COX-2 inhibitory activities, and two diphenylketone derivatives (5 and 6) exhibited obvious COX-2 inhibitory activities, with the IC50 values being 5.8 and 3.4 µM, respectively.

8.
Sci Rep ; 10(1): 5765, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32238870

RESUMO

Although differentiated thyroid carcinoma (DTC) has a good prognosis and survival rate, long-term medication and recurrence monitoring might be needed. The factors that affect postoperative health-related quality of life (HRQoL) in patients with DTC in different regions remain unclear or conflicting. The purpose of this study was to assess the factors that influence the HRQoL of DTC patients after surgery. This study selected 174 patients with DTC who underwent thyroidectomy. Additionally, 174 participants who were matched by age, gender, and socioeconomic status were recruited from the population as the control group. Both the DTC and control population groups were invited to answer the HRQoL questionnaire SF-36. Scores on seven domains of the HRQoL including role-physical (RP), bodily pain (BP), general health (GH), vitality (VT), social functioning (SF), role-emotional (RE), and mental health (MH), were significantly lower for DTC patients than for the control population. The patients with no comorbidities had much higher scores on the 8 domains of the SF-36 than DTC patients with two or more comorbidities (all P < 0.05). Hypertension, diabetes and depression were the predictive factors of a poor Physical Component Summary (PCS) score and diabetes and depression were predictive factors of the Mental Component Summary (MCS) score at one year of follow-up (all P < 0.05). HRQoL is significantly influenced by many sociodemographic and clinical factors. Hypertension, diabetes and depression had a negative impact on HRQoL in DTC patients. More attention and targeted intervention should be given to DTC patients after surgery to improve quality of life.

9.
Nat Prod Res ; : 1-5, 2020 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-32264697

RESUMO

Twelve indole alkaloids, including α-cyclopiazonic acid (CPA) (1), nine 2-oxo indole CPA derivatives (2-10), and two open-ring indole CPA derivatives (11 and 12), were isolated from the fermentation broth of a deep-sea derived fungus Aspergillus sp. SCSIO 41024. Their structures and absolute configurations were elucidated mainly by using extensive NMR spectroscopic, mass spectrometric and single crystal X-ray diffraction analysis. To the best of our knowledge, the crystallographic data of 3 and 7 were firstly reported, and the absolute configuration of 3 was confirmed for the first time by the single crystal X-ray diffraction analysis. Most isolated compounds were tested for their antimicrobial, antitumor and radical scavenging activities. In addition, compounds 1, 2 and 11 showed moderate antioxidative activity against DPPH with IC50 values of 190.1, 31.9, 228.4 µg/mL, respectively.

10.
J Biosci Bioeng ; 2020 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-32224010

RESUMO

Macrolactins (MLNs) have attracted considerable attention due to their antibacterial and antiviral properties. Here, the MLN production of Bacillus sp. strain IMDGX0108 was improved using a breeding strategy of atmospheric room temperature plasma (ARTP) technique. Combining with a selection procedure based on the colony morphology and specific growth rate index (SGRI), two genetically stable mutants A29 and A72 were identified. The MLN production of A29 and A72 was 35.2% and 52.8% greater than that of the parent strain, respectively. The best-performing mutant A72 was subjected to RNA-sequence analysis. Five pathways were significantly enriched, and fatty acid bioprocesses might play an important role in improving the production of MLNs. The combined strategy developed herein (i.e., ARTP mutation plus an efficient screening procedure) might be an appropriate method by which to obtain strains overproducing MLNs.

11.
Korean J Parasitol ; 58(1): 37-46, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32145725

RESUMO

Livestock husbandry is vital to economy of the Tarim Basin, Xinjiang Autonomous Region, China. However, there have been few surveys of the distribution of ixodid ticks (Acari: Ixodidae) and tick-borne pathogens affecting domestic animals at these locations. In this study, 3,916 adult ixodid ticks infesting domestic animals were collected from 23 sampling sites during 2012-2016. Ticks were identified to species based on morphology, and the identification was confirmed based on mitochondrial 16S and 12S rRNA sequences. Ten tick species belonging to 4 genera were identified, including Rhipicephalus turanicus, Hyalomma anatolicum, Rh. bursa, H. asiaticum asiaticum, and Rh. sanguineus. DNA sequences of Rickettsia spp. (spotted fever group) and Anaplasma spp. were detected in these ticks. Phylogenetic analyses revealed possible existence of undescribed Babesia spp. and Borrelia spp. This study illustrates potential threat to domestic animals and humans from tick-borne pathogens.

12.
Molecules ; 25(5)2020 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-32182966

RESUMO

Cytochalasans have continuously aroused considerable attention among the chemistry and pharmacology communities due to their structural complexities and pharmacological significances. Sixteen structurally diverse chaetoglobosins, 10-(indol-3-yl)-[13]cytochalasans, including a new one, 6-O-methyl-chaetoglobosin Q (1), were isolated from the coral-associated fungus Chaetomium globosum C2F17. Their structures were accomplished by extensive spectroscopic analysis combined with single-crystal X-ray crystallography and ECD calculations. Meanwhile, the structures and absolute configurations of the previously reported compounds 6, 12, and 13 were confirmed by single-crystal X-ray analysis for the first time. Chaetoglobosins E (6) and Fex (11) showed significant cytotoxicity against a panel of cancer cell lines, K562, A549, Huh7, H1975, MCF-7, U937, BGC823, HL60, Hela, and MOLT-4, with the IC50 values ranging from 1.4 µM to 9.2 µM.

13.
Bioorg Chem ; 96: 103646, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32036160

RESUMO

Three new compounds, including two new 3,4,6-trisubstituted α-pyrone derivatives, chrysopyrones A and B (1 and 2), and one new indolyl diketopiperazine derivative, penilline C (3), along with twelve known compounds (4-15), were isolated and identified from the fungus Penicillium chrysogenum SCSIO 07007, separated from deep-sea hydrothermal vent environment sample collected from the Western Atlantic. Their structures and absolute configurations were determined by extensive spectroscopic analysis and electronic circular dichroism (ECD) calculations. All of the isolated compounds (1-15) were evaluated for their cytotoxic, antibacterial activities and enzyme inhibitory activities against acetylcholinesterase (AChE), α-glycosidase, and protein tyrosine phosphatase 1B (PTP1B). Among them, new compounds chrysopyrones A and B (1 and 2) displayed obvious inhibitory activities against PTP1B with IC50 values of 9.32 and 27.8 µg/mL, respectively. Furthermore, molecular docking was performed to investigate the inside perspective of the action in PTP1B enzyme.

14.
Chemosphere ; 247: 125965, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32069730

RESUMO

Phosphorous (P) fertilization is an important agronomic practice, but its role in enhancing phytoremediation efficacy and mediating detoxification has rarely been reported in environmental remediation studies. In this study, a pot experiment was undertaken to assess: firstly, the effect of P on phytoextraction of Cu by Ricinus communis L.; secondly, the potential mechanisms by differentiating the effects of the plant from that of P fertilizer (Ca(H2PO4)2); and thirdly, the role of P in physiological detoxification. Results showed that the application of P fertilizer significantly (p ≤ 0.05) increased the plant biomass as well as the Cu concentrations in plant tissues. This enhanced the phytoremediation efficiency represented by the total Cu extraction (up to 121.3 µg Cu plant-1). Phosphorous (P) fertilizer led to a negligible decline in soil pH (0.2 units) but significantly (p ≤ 0.05) reduced the concentrations of soil available in Cu and Fe, due to the formation of insoluble Cu/Fe-phosphate precipitates. Nevertheless, P fertilizer still improved the accumulation and extraction of Cu by R. communis, most likely attributable to the Fe-deficiency induced by applied P fertilizer. Moreover, the application of P fertilizer revealed a significant reduction in MDA, and a profound (p ≤ 0.05) elevation in the amount of photosynthetic pigments, GSH and AsA, along with the enhanced activities of antioxidative enzymes (SOD, POD, and CAT). In this way, Cu toxicity was alleviated. P fertilizers not only enhance the phytoremediation efficiency of Cu-contaminated soils by R. communis, but they also facilitate detoxification, which improves our understanding of the role of P in phytoremediation technologies.


Assuntos
Biodegradação Ambiental , Cobre/metabolismo , Fertilizantes , Fósforo/farmacologia , Ricinus/efeitos dos fármacos , Antioxidantes/análise , Antioxidantes/farmacologia , Biomassa , Cobre/farmacocinética , Recuperação e Remediação Ambiental/métodos , Inativação Metabólica , Fotossíntese , Ricinus/metabolismo , Solo/química , Poluentes do Solo/análise , Poluentes do Solo/farmacocinética
15.
Artigo em Inglês | MEDLINE | ID: mdl-32053082

RESUMO

BACKGROUND: LCL161, a Smac's small molecule mimetic, can bind to a variety of IAPs and activate Caspases. We found that, on its own, LCL161induces apoptosis of drug-resistant breast cancer cells by binding to a variety of IAPs and activating Caspases. However, when LCL161 is used in combination with Caspase Inhibitors (CI), its capacity induce apoptosis of breast cancer cells is enhanced. OBJECTIVE: To carry out proteomic and bioinformatics analysis of LCL161 in combination with CI. We aim to identify the key proteins and mechanisms of breast cancer drug-resistant apoptosis, thereby aiding in the breast cancer drug resistance treatment and identification of drug targeting markers. METHODS: Cell culture experiments were carried out to explore the effect of LCL161 combined with CI on proliferation of breast cancer drug-resistant cells. Proteomic analysis was carried out to determine the protein expression differences between breast cancer drug-resistant cells and LCL161 combined with CI treated cells. Bioinformatics analysis was carried out to determine its mechanism of action. Validation of proteomics results was done using Parallel reaction monitoring (PRM). RESULTS: Cell culture experiments showed that LCL161 in combination with CI can significantly promote the apoptosis of breast cancer drug-resistant cells. Up-regulation of 92 proteins and down regulation of 114 proteins protein were noted, of which 4 were selected for further validation. CONCLUSION: Our results show that, when LCL161 combined with CI can promote the apoptosis of drug-resistant breast cancer cells by down-regulation of RRM2, CDK4 and ITGB1 expression through Cancer pathways, p53 or PI3K-AKT signaling pathway. In addition, the expression of CDK4, RRM2 and CDC20 can be down-regulated by the nuclear receptor pathway to affect DNA transcription and replication, thereby promoting apoptosis of breast cancer drug-resistant cells.

16.
Int J Biochem Cell Biol ; 121: 105696, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32001362

RESUMO

Bovine mammary fibrosis represents a considerable health problem of cows, primarily indicated by lactation failure. Staphylococcus aureus (S. aureus) can cause mammary damage, this multifactorial disease necessitates to identify how and to what extent molecular pathogen defense mechanisms prevent bacterial infections in bovine mammary gland. In this study, we have aimed to determine the transcriptional responses in bovine mammary fibroblasts (BMFBs) induced by S. aureus using bioinformatics analysis to determine whether mRNA expression profile changes between BMFBs activation and quiescence. Established primary BMFBs obtained from healthy Holstein bovine were induced 106 CFU/mL heat-inactivated S. aureus and total RNA was isolated 6 h after treatment. The 574 DEGs were involved in gene ontology (GO) that were immune response, apoptotic process, extracellular region, receptor binding, endopeptidase activity and protein kinase activity et al. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, distinct pathway contained signaling molecules common to various inflammatory and fibrotic pathways were Pathways in cancer, Cytokine-cytokine receptor interaction, PI3K-Akt signaling pathway, TNF signaling pathway, MAPK signaling pathway and Toll-like receptor signaling pathway. The BMFBs was treated with heat-inactivated S. aureus (106 CFU/mL) and also with pharmacological inhibitors of ERK1/2, P38 MAPK and JNK. The MMP-2 activity were examined gelatin zymography, MMP-2, TIMP-1, -2 and PLAU/PAI-1 protein expression were examined in vitro by western blot. The MMP-2 activity was significantly inhibited by simultaneous inhibition of ERK1/2, P38 MAPK and JNK, and MMP-2, TIMP-1,-2 and PLAU/PAI-1 protein expression were significantly decreased by inhibiting ERK1/2, P38 MAPK or JNK. This suggested a crosstalk between the ERK1/2, P38 MAPK or JNK signaling pathways in regulating extracellular matrix metabolism in the BMFBs with S. aureus. Our study complement our initial study on S. aureus-induced responses by fibrosis-associated genes in BMFBs. This may lead to development of novel therapeutic targets to control bovine mammary fibrosis induced by S. aureus.

18.
Steroids ; 157: 108594, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32068077

RESUMO

Five cholic acid derivatives (including allo-ω-muricholic acid and CDCA) were synthesized from hyodeoxycholic acid via selective oxidation of C3- or C6-hydroxyl groups by IBX and NBS oxidants and stereocontrolled conversion. The hydroxyl group could be introduced through hydrolyzing α-Br keto with K2CO3 aqueous solution or through oxidizing the double bond by monoperoxyphthalic acid. The reduction of C6-O6 carbonyl to methylene could undergo with PTSH, NaBH3CN and ZnCl2 only at 5ß configuration. A feasible synthetic route of CDCA from HDCA has been established to avoid the epimerization with the yield of 45% (8 steps). These strategies provided good yields, stereoselectivity and reproducibility for the preparation of cholic acid derivates and CDCA.

19.
Nat Prod Res ; : 1-8, 2020 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-31984766

RESUMO

The present work reports the bioassay-guided isolation of four bioactive fatty acid derivatives involving a new butenolide, namely sinulolide I (1) together with three known metabolites (2-4) from the deep-sea sediment derived fungus Aspergillus terreus SCSIO 41202. The chemical structure of compound 1 was elucidated based on extensive spectroscopic methods (1D/2D NMR and HR-ESI-MS), optical rotation and circular dichroism analyses, while the structures of the known compounds (2-4) were established by comparison of NMR spectral data with those reported in literature. All of these four compounds (1-4) exhibited significant antifungal activity against citrus postharvest pathogen Penicillium italicum (MICs around 0.031-0.125 mg/mL).

20.
J Cell Mol Med ; 24(6): 3384-3398, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31981312

RESUMO

Recent studies have demonstrated that commercially available lipid-lowering drugs cause various side effects; therefore, searching for anti-hyperlipidaemic compounds with lower toxicity is a research hotspot. This study was designed to investigate whether the marine-derived compound, 5-hydroxy-3-methoxy-5-methyl-4-butylfuran-2(5H)-one, has an anti-hyperlipidaemic activity, and the potential underlying mechanism in vitro. Results showed that the furanone had weaker cytotoxicity compared to positive control drugs. In RAW 264.7 cells, the furanone significantly lowered ox-LDL-induced lipid accumulation (~50%), and its triglyceride (TG)-lowering effect was greater than that of liver X receptor (LXR) agonist T0901317. In addition, it significantly elevated the protein levels of peroxisome proliferator-activated receptors (PPARα) and ATP-binding cassette (ABC) transporters, which could be partially inhibited by LXR antagonists, GSK2033 and SR9243. In HepG2 cells, it significantly decreased oleic acid-induced lipid accumulation, enhanced the protein levels of low-density lipoprotein receptor (LDLR), ABCG5, ABCG8 and PPARα, and reduced the expression of sterol regulatory element-binding protein 2 (~32%). PPARα antagonists, GW6471 and MK886, could significantly inhibit the furanone-induced lipid-lowering effect. Furthermore, the furanone showed a significantly lower activity on the activation of the expression of lipogenic genes compared to T0901317. Taken together, the furanone exhibited a weak cytotoxicity but had powerful TC- and TG-lowering effects most likely through targeting LXRα and PPARα, respectively. These findings indicate that the furanone has a potential application for the treatment of dyslipidaemia.

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