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1.
Zhongguo Zhong Yao Za Zhi ; 46(12): 3165-3170, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34467709

RESUMO

Nucleic acid aptamers, broad-spectrum target-specific single-stranded oligonucleotides, serve as molecules in targeted therapy, targeted delivery and disease diagnosis for the treatment of tumor or microbial infection and clinical detection. Due to the existence of components in the use of traditional Chinese medicine(TCM), the target is difficult to concentrate and the specificity of treatment is poor. The effective components of TCM are toxic components, so a highly sensitive detection method is urgently needed to reduce the toxicity problem at the same time. The combined application of TCM and modern medical treatment strategy are difficult and cannot improve the therapeutic effect. Aptamers, advantageous in biosensors, aptamer-nanoparticles for targeted drug delivery, and aptamer-siRNA chimeras, are expected to connect Chinese medicinals with nanotechnology, diagnostic technology and combined therapies. We summarized the preparation, screening, and modification techniques of nucleic acid aptamers and the biomedical applications and advantages in therapy, targeting, and diagnosis, aiming at providing a reference for the in-depth research and development in TCM.


Assuntos
Aptâmeros de Nucleotídeos , Ácidos Nucleicos , Sistemas de Liberação de Medicamentos , Medicina Tradicional Chinesa , RNA Interferente Pequeno
2.
Zhongguo Zhong Yao Za Zhi ; 43(22): 4519-4527, 2018 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-30593248

RESUMO

This present study was to investigate the metabolism and excretion of characteristic polyphenols such as flavonoids and coumarins in urine and feces of rats after intragastric administration of ethanol extracts of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium. The urine and feces of rats were collected after intragastric administration of 70% ethanol extracts of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium. Rapid resolution liquid chromatography coupled with quadrupole tandem mass spectrometry (RRLC-QqQ-MSn) was applied to compare the contents of polyphenols in ethanol extract, urine and feces. By comparing with reference substance, 30 polyphenols were identified from the ethanol extracts of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium, including flavone glycosides, flavones, flavonone glycosides, flavonones, flavonol glycosides, polymethoxyflavones, coumarins, and limonoids and so on. The detection of various types of compounds showed differences in contents between the intestinal metabolism and excretion in the feces after systemic circulatory metabolism and renal excretion. The results showed that the polymethoxyflavones and flavonones were primarily excreted through urine, and the flavonone glycosides and limonoids were primarily excreted through feces. However, coumarins were hardly detected in feces and urine, indicating that coumarins may be metabolized in the body.


Assuntos
Fezes , Animais , Citrus , Medicamentos de Ervas Chinesas , Flavonoides , Ratos , Espectrometria de Massas em Tandem
3.
J Chromatogr Sci ; 56(6): 541-554, 2018 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-29635273

RESUMO

Fuzi Lizhong pill (FLP) is used to treat gastritis, and the monarch drug of it is Aconiti Lateralis Radix Praeparata (Fuzi, aconite roots) which is a toxic herbal medicine. To better control the safety and quality of FLP, an effective method to analyze the contents of 16 toxic and bioactive components using rapid resolution liquid chromatography-tandem triple-quadrupole mass spectrometer was established. The 16 constituents included aconine, mesaconine, hypaconitine, benzoylaconine, benzoylmesaconine, benzoylhypaconine, adenosine, liquiritin, liquiritigenin, glycyrrhizic acid, isoliquiritigenin, 6-gingerol, atractylenolide III, atractylenolide I, atractylenolide II and glycyrrhetic acid. Ideal separation was performed using gradient elution in 13 min by optimized conditions. All the isomerides were isolated to baseline. The improved method with a polarity switch in contiguous time segments could analyze the five types of components, including polar and nonpolar compounds, without decreasing sensitivity. The proposed method was fully validated. The results revealed that contents of six alkaloids from Fuzi were significantly different among the samples. Using the established method and multivariate statistical method, the quality consistency of two dosage forms of FLP from different companies were analyzed. The optimized method could be used for the quality control of FLP and investigate index compound variation between two dosage forms.


Assuntos
Cromatografia Líquida/métodos , Medicamentos de Ervas Chinesas , Espectrometria de Massas em Tandem/métodos , Alcaloides/análise , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/normas , Análise dos Mínimos Quadrados , Limite de Detecção , Modelos Lineares , Controle de Qualidade , Curva ROC , Reprodutibilidade dos Testes
4.
Molecules ; 22(10)2017 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-29027971

RESUMO

This manuscript elaborates on the establishment of a chemotaxonomic classification strategy for closely-related Citrus fruits in Traditional Chinese Medicines (TCMs). UPLC-Q-TOF-MS-based metabolomics was applied to depict the variable chemotaxonomic markers and elucidate the metabolic mechanism of Citrus TCMs from different species and at different ripening stages. Metabolomics can capture a comprehensive analysis of small molecule metabolites and can provide a powerful approach to establish metabolic profiling, creating a bridge between genotype and phenotype. To further investigate the different metabolites in four closely-related Citrus TCMs, non-targeted metabolite profiling analysis was employed as an efficient technique to profile the primary and secondary metabolites. The results presented in this manuscript indicate that primary metabolites enable the discrimination of species, whereas secondary metabolites are associated with species and the ripening process. In addition, analysis of the biosynthetic pathway highlighted that the syntheses of flavone and flavone glycosides are deeply affected in Citrus ripening stages. Ultimately, this work might provide a feasible strategy for the authentication of Citrus fruits from different species and ripening stages and facilitate a better understanding of their different medicinal uses.


Assuntos
Citrus/química , Frutas/química , Medicina Tradicional Chinesa , Metabolômica/classificação , Cromatografia Líquida , Citrus/classificação , Citrus/metabolismo , Frutas/classificação , Frutas/metabolismo , Genótipo , Glicosídeos/química , Glicosídeos/metabolismo , Humanos , Fenótipo
5.
Zhongguo Zhong Yao Za Zhi ; 41(5): 874-878, 2016 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-28875642

RESUMO

In this paper, an HPLC-QqQ-MS method for determination of 5 different ginsenosides of Panax japonica collected from different cultivated geographic regions was established. The separation was performed on a Zorbax XDB-C18 (4.6 mm×100 mm, 1.8 µm) column with the gradient elution of acetonitrile (contained 0.1% formic acid)-0.1% formic acid water. The flow rate was 0.5 mL•min⁻¹. The colunm temperature was maintained at 30 ℃. The analytes were detected using electrospray ionization (ESI) in multiple reaction monitoring (MRM) modes. Reaction selected ions were 203.2 for ginsenoside Re, 202.9 for ginsenoside Rg1, 365.0 for ginsenoside Rf, 789.1 for ginsenoside Rd, 360.9 for ginsenoside Ro. Ginsenosides Re, ginsenosides Rg1, ginsenosides Rf, ginsenosides Rd, ginsenosides Ro had good linearity in the ranges of 3.33-66.60 µg (r=0.999 1),2.83-56.54 µg (r=0.999 2), 0.32-6.51 µg (r=0.999 2), 12.55-251.00 µg (r=0.999 3), 0.85-16.90 µg (r=0.999 5), respectively. The results of recovery were among 100.8% to 104.6%, and the values of RSD were blow 3.0%. This method is simple, reliable and accurate, and can provide basis for P. japonica basic research.


Assuntos
Medicamentos de Ervas Chinesas/química , Ginsenosídeos/química , Panax/química , China , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/isolamento & purificação , Ginsenosídeos/isolamento & purificação , Espectrometria de Massas , Panax/classificação , Panax/crescimento & desenvolvimento
6.
Zhongguo Zhong Yao Za Zhi ; 41(17): 3272-3278, 2016 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-28920382

RESUMO

This study is to establish an HPLC fingerprint by HPLC-DAD method and simultaneous quantitative analysis of 17 components of 18 batches of Citrus aurantium and 10 batches of C. sinensis. The separation was performed on an Agilent Poroshell 120 SB-C18 (4.6 mm×100 mm,2.7 µm) column with the gradient elution of methanol-0.1% formic acid water, the flow was 0.6 mL•min⁻¹. The detection wavelength was set at 318 nm. The column temperature was maintained at 30 ℃. The data calculation was performed with similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004A) together with SIMCA-P 13.0 software to clarify the differential marker between these two different species of Aurantii Fructus Immaturus. This method has good precision stability and repeatability that could provide basis for quality control and evaluation of Aurantii Fructus Immaturus.


Assuntos
Citrus/química , Medicamentos de Ervas Chinesas/química , Cromatografia Líquida de Alta Pressão , Controle de Qualidade
7.
Phytochemistry ; 72(1): 115-25, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21112063

RESUMO

Eleven prenylated C(6)-C(3) compounds, illioliganpyranone A (1), illioliganfunone A-D (2-5), and illioliganone D-I (6-11), together with five known prenylated C(6)-C(3) compounds (12-16), were isolated from roots of Illicium oligandrum. The structures of 1-11 were elucidated by spectroscopic methods including 1D and 2D NMR, HRESIMS, and CD experiments. Possible biosynthetic pathways to compounds 1-16 derived from a common precursor of 5-allylbenzene-1,2,4-triol were postulated. All compounds were evaluated for cytotoxic activities against five human cancer cell lines (HCT-8, Bel-7402, BGC-823, A549 and A2780). Compound 15 exhibited significant cytotoxicity against HCT-8, BGC-823, A549, and A2780 cell lines with IC(50) values of 0.30-2.57 µM. Compound 16 showed moderate selective cytotoxicity against sensitive A2780 cells with IC(50) value of 1.38 µM.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Benzodioxóis/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Illicium/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Benzodioxóis/química , Benzodioxóis/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Raízes de Plantas/química , Prenilação
8.
Zhongguo Zhong Yao Za Zhi ; 31(4): 309-11, 2006 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-16706022

RESUMO

OBJECTIVE: To study the chemical constituents in roots of Heracleum rapula. METHOD: The constituents were isolated by column chromatography on silica gel and ODS, and identified by spectroscopic methods. RESULT: Nine compounds, osthol ( I ), bergapten (II), xanthotoxin (III), isopimpinellin (IV), imperatorin (V), isoimperatorin (VI), cnidilin (VII), phellopterin (VIII), rivulobirin A (IX) were isolated and identified. CONCLUSION: Compounds VI, VII, VIII, IX were isolated from this plant for the first time.


Assuntos
Cumarínicos/isolamento & purificação , Furocumarinas/isolamento & purificação , Heracleum/química , Plantas Medicinais/química , Cumarínicos/química , Furocumarinas/química , Raízes de Plantas/química
9.
Zhongguo Zhong Yao Za Zhi ; 31(3): 194-6, 2006 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-16572995

RESUMO

OBJECTIVE: To develop an HPLC method for determination of components in root of Saposhnikovia divaricata. METHOD: The separation of four components was achieved on a reversed-phase Alltima C18 column with MeOH-H2O gradient elution at a flow rate of 1.0 mL x min(-1). RESULT: Six samples were determined and there is no distinct difference between the contents determined by the new and old methods. CONCLUSION: The new method is more simple and convenient than the old method.


Assuntos
Apiaceae/química , Medicamentos de Ervas Chinesas/análise , Monossacarídeos/análise , Plantas Medicinais/química , Xantenos/análise , Cromatografia Líquida de Alta Pressão/métodos , Raízes de Plantas/química
10.
Zhongguo Zhong Yao Za Zhi ; 30(3): 177-9, 2005 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-15719631

RESUMO

OBJECTIVE: To establish a quality standard of Peucedanum praeruptorum for Chinese Pharmacopoeia (2005). METHOD: The presence of (+/-)-praeruptorin A were identified and assayed by TLC and HPLC, respectively. RESULT: Linearity of marker was obtained over the range of 0.17696-0.88480 microg (r=0.9999). The average recovery rate was 99.72% (RSD=0.40%). CONCLUSION: TLC is specific. The method of quantity is accurate, reappearance, simple, rapid, and suitable for the quality control of P. praeruptorum.


Assuntos
Apiaceae/química , Cumarínicos/análise , Plantas Medicinais/química , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Delgada , Farmacognosia , Raízes de Plantas/química , Controle de Qualidade
11.
Zhongguo Zhong Yao Za Zhi ; 29(11): 1030-2, 2004 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-15656129

RESUMO

OBJECTIVE: To establish a quantitative method of determination of carvacrol and thymol in Mosla chinensis. METHOD: The sample was extracted with 95% ethanol, ODS column was used with methanol-water-acetic acid (60:40:2) as mobile phase. The detection wavelength was set at 274 nm. RESULT: The linearities of carvacrol and thymol were respectively in the range of 0.23-2.15 microg (r = 0.9999) and 0.39-2.36 microg (r = 0.9999); the average recoveries were 99.9% (RSD 1.4%) and 98.6% (RSD 1.3%); the RSD of repeatability were 1.1% and 1.6%. CONCLUSION: The method is reliable, and can be used for quality control of M. chinensis.


Assuntos
Lamiaceae/química , Monoterpenos/análise , Plantas Medicinais/química , Timol/análise , Cromatografia Líquida de Alta Pressão , Cimenos , Componentes Aéreos da Planta/química , Controle de Qualidade
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