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1.
Fertil Steril ; 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32741620

RESUMO

OBJECTIVE: To assess whether trophectoderm biopsy has any impact on the level of serum ß-human chorionic gonadotropin (ß-hCG) in early pregnancies. DESIGN: Retrospective cohort study. SETTING: University-affiliated reproductive medical center. PATIENT(S): Three hundred and eighty-three women undergoing 396 frozen embryo transfer (FET) cycles with preimplantation genetic testing (PGT), and 353 women undergoing 465 FET cycles with in vitro fertilization or intracytoplasmic sperm injection, all women having positive serum ß-hCG results on the 12th day after blastocysts transfers. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Serum ß-hCG levels on the 12th day after warmed blastocyst transfer and perinatal outcomes of clinical pregnancy. RESULTS: The diagnostic threshold of serum ß-hCG levels on the 12th day after FET for prediction of a live birth was 368.55 mIU/mL with an area under the curve of 0.791 (0.729∼0.853) in the biopsy group, which was lower than the 411.45 mIU/mL in the control group. The average level of serum ß-hCG in the biopsy group with clinical pregnancies was statistically significantly lower than that of the control group: 703.10 (569.63) versus 809.20 (582.00), respectively. No statistically significant differences in perinatal outcomes, including gestational age, hypertensive disorder in pregnancy, and neonatal malformation, were found between the two groups. CONCLUSION(S): Trophectoderm biopsy may reduce the level of serum ß-hCG in early pregnancies (the 12th day after embryo transfer), but no increased risk was found of adverse perinatal outcomes after trophectoderm biopsy.

2.
Sci Total Environ ; 730: 138888, 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32402961

RESUMO

Eastern China has been facing severe winter haze pollution due mainly to secondary aerosol. Existing studies have suggested that stagnant weather or fast chemical production led to frequent haze in this region. However, few works focus on the linkage between secondary production of sulfate, nitrate, and ammonium (SNA) and synoptic conditions, and their joint contribution to PM2.5. In this study, by combining in-situ measurements on meteorology and aerosol chemical composition at three main cities together with a regional model with improved diagnose scheme, we investigated the chemical formation and accumulation of main secondary composition, i.e. SNA under typical synoptic conditions. It is indicated that SNA did play a vital role in haze pollution across eastern China, contributing more than 40% to PM2.5 mass concentration. As most fast developing region, the Yangtze River Delta (YRD) was slightly polluted during stable weather with local chemical production accounting for 61% SNA pollution. While under the influence of cold front, the pollution was aggravated and advection transport became the predominant contributive process (85%). Nevertheless, the chemical production of SNA was notably enhanced due to the uplift of air pollutant and elevated humidity ahead of the cold front, which then facilitated the heterogeneous and aqueous-phase oxidation of precursors. We also found the substantial difference in the phase equilibrium of nitrate over the land surface and ocean due to changes in temperature, ammonia availability and dry deposition. This study highlights the close link between synoptic weather and chemical production, and the resultant vertical and spatial heterogeneity of pollution.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32373070

RESUMO

Giant pandas represent one of the most endangered species worldwide, and their reproductive capacity is extremely low. They have a relatively long gestational period, mainly because embryo implantation is delayed. Giant panda cubs comprise only a small proportion of the mother's body weight, making it difficult to determine whether a giant panda is pregnant. Timely determination of pregnancy contributes to the efficient breeding and management of giant pandas. Meanwhile, metabolomics studies the metabolic composition of biological samples, which can reflect metabolic functions in cells, tissues, and organisms. This work explored the urinary metabolites of giant pandas during pregnancy. A sample of 8 female pandas was selected. Differences in metabolite levels in giant panda urine samples were analyzed via ultra-high-performance liquid chromatography/mass spectrometry comparing pregnancy to anoestrus. Pattern recognition techniques, including partial least squares-discriminant analysis and orthogonal partial least squares-discriminant analysis, were used to analyze multiple parameters of the data. Compared with the results during anoestrus, multivariate statistical analysis of results obtained from the same pandas being pregnant identified 16 differential metabolites in the positive-ion mode and 43 differential metabolites in the negative-ion mode. The levels of tryptophan, choline, kynurenic acid, uric acid, indole-3-acetaldehyde, taurine, and betaine were higher in samples during pregnancy, whereas those of xanthurenic acid and S-adenosylhomocysteine were lower. Amino acid metabolism, lipid metabolism, and organic acid production differed significantly between anoestrus and pregnancy. Our results provide new insights into metabolic changes in the urine of giant pandas during pregnancy, and the differential levels of metabolites in urine provide a basis for determining pregnancy in giant pandas. Understanding these metabolic changes could be helpful for managing pregnant pandas to provide proper nutrients to their fetuses.

4.
Cryobiology ; 94: 95-99, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32304703

RESUMO

Sperm cryopreservation is an essential approach for assisted reproduction and genetic resources conservation in captive giant pandas. Cryopreservation, however, leads to a significant decrease in sperm quality and, consequently, a low fertilization rate. Therefore, it is mandatory to disclose more suitable and efficient freezing strategies for sperm cryopreservation. In the present study, we compared for the first time the performance of two commercial freeze extender (INRA96 versus TEST) freezing methods on post-thawed semen quality. Semen cryopreserved with the INRA96 showed better total motility (73.00 ± 4.84% vs 57.56 ± 3.60%, P < 0.001), membrane integrity (60.92 ± 2.27% vs 40.53 ± 2.97%, P < 0.001) and acrosome integrity (90.39 ± 2.74% vs 84.26 ± 4.27%, P < 0.05) than stored with TEST. There was no significant difference in DNA integrity after thawing between the two extenders (95.69 ± 3.60% vs 94.26 ± 4.84%). In conclusion, the INRA96 method showed to be better for giant panda sperm cryopreservation and should therefore be recommended for use in order to increase success of artificial insemination.

5.
Zoology (Jena) ; 140: 125775, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32251890

RESUMO

Mesenchymal stem cells (MSC) have strong therapeutic potential due to their capacity for self-renewal and multilineage differentiation. MSCs can also be useful in preserving the current genetic diversity of endangered wildlife. To date, MSCs from various species have been studied, but only a few species of endangered wild animals have been reported. Adult bone marrow (BM) is a rich source of mesenchymal stem cells. The aim of this study was to isolate and characterize MSCs derived from the BM of red pandas. Red panda BM-MSCs isolated from five individuals were fibroblast-like cells, similar to other species. Cultured BM-MSCs with normal karyotype were negative for the hematopoietic line marker CD34 and the endothelial cell marker CD31 but were positive for MSC markers, including CD44, CD105 and CD90. RT-PCR and western blot analysis showed self-renewal and pluripotency genes, including Oct4, Sox2 and Klf4, were also expressed in red panda BM-MSCs. Finally, red panda BM-MSCs had the potential for differentiation into osteogenic, adipogenic and neuron-like cells by using a combination of previously reported protocols for other species. We have therefore demonstrated that cells harvested from red panda bone marrow are capable of extensive in vitro multiplication and multilineage differentiation, which is an essential step toward their use in the preservation of red pandas biological diversity and future studies on MSC applications in endangered species.

6.
Clin Respir J ; 14(7): 631-637, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32125778

RESUMO

BACKGROUND: The association of clinical risk factors, in particular deep vein thrombosis (DVT), with risk stratification for pulmonary embolism (PE) remains to be identified. We therefore aimed to establish the relationship between risk stratification of PE patients and DVT of lower extremities. METHODS: In this retrospective study, 93 out of 485 PE patients with uncompleted clinical data were excluded, resulting in 392 patients included for analysis. Based on the ESC criteria, 24, 171, and 197 patients were categorized into high (6.1%), intermediate (43.6%), and low risk (50.3%) subgroups, respectively. RESULTS: DVT was detected in 304 patients (77.6%). The incidence of DVT in patients with high and intermediate risk PE was much lower than in those patients with low risk PE (67.2% vs 87.8%, P < .0001). Further analyses of the 304 patients with DVT showed higher incidence of high and intermediate risk PE in patients with isolated distal DVT than proximal DVT (59.0% vs 39.1%, P = .005), with asymptomatic DVT than symptomatic DVT (63.0% vs 36.8%, P < .0001), and with bilateral DVT than unilateral DVT (54.5% vs 39.9%, P = .03). Stepwise logistic regression showed that symptomatic or asymptomatic DVT was an independent risk factor for risk stratification of PE patients with DVT (0.320, 95% confidence interval, 0.186-0.550). CONCLUSIONS: Patients with high and intermediate risk PE presented lower incidence of DVT compared with patients with low risk PE. In PE patients with comorbid DVT, asymptomatic DVT is an independent risk factor for high and intermediate risk of PE.

7.
Oral Oncol ; 104: 104625, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32151995

RESUMO

OBJECTIVES: To investigate whether dosiomics can benefit to IMRT treated patient's locoregional recurrences (LR) prediction through a comparative study on prediction performance inspection between radiomics methods and that integrating dosiomics in head and neck cancer cases. MATERIALS AND METHODS: A cohort of 237 patients with head and neck cancer from four different institutions was obtained from The Cancer Imaging Archive and utilized to train and validate the radiomics-only prognostic model and integrate the dosiomics prognostic model. For radiomics, the radiomics features were initially extracted from images, including CTs and PETs, and selected on the basis of their concordance index (CI) values, then condensed via principle component analysis. Lastly, multivariate Cox proportional hazards regression models were constructed with class-imbalance adjustment as the LR prediction models by inputting those condensed features. For dosiomics integration model establishment, the initial features were similar, but with additional 3-dimensional dose distribution from radiation treatment plans. The CI and the Kaplan-Meier curves with log-rank analysis were used to assess and compare these models. RESULTS: Observed from the independent validation dataset, the CI of the model for dosiomics integration (0.66) was significantly different from that for radiomics (0.59) (Wilcoxon test, p=5.9×10-31). The integrated model successfully classified the patients into high- and low-risk groups (log-rank test, p=2.5×10-02), whereas the radiomics model was not able to provide such classification (log-rank test, p=0.37). CONCLUSION: Dosiomics can benefit in predicting the LR in IMRT-treated patients and should not be neglected for related investigations.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31794397

RESUMO

Scene text in the environment is complicated. It can exist in arbitrary text fonts, sizes or shapes. Although scene text detection has witnessed considerable progress in recent years, the detection of text with complex shapes, especially curved text, remains challenging. Datasets with adequate samples to overcome the problem presented by curved text (or other irregularly shaped text) have been introduced only recently; however, the performance of the reported methods on these datasets is unsatisfactory. Therefore, detecting arbitrarily shaped text remains a challenging. This motivated us to propose the Mask Tightness Text Detector (Mask TTD) to improve text detection performance. Mask TTD uses a tightness prior and text frontier learning to enhance pixel-wise mask prediction. In addition, it achieves mutual promotion by integrating a branch for the polygonal boundary of each text region, which significantly improves the detection performance of arbitrarily shaped text. Experiments demonstrate that Mask TTD can achieve state-ofthe-art performance on existing curved text datasets (CTW1500, Total-text, and CUTE80) and three common benchmark datasets (RCTW-17, MSRA-TD500, and ICDAR 2015). It is worth mentioning that on CTW1500, our method can outperform previous methods, especially at higher intersection over union (IoU) thresholds (16% higher than the next-best method with an IoU threshold of 0.8), which demonstrates its potential for tight text detection. Moreover, on the largest Chinese-based dataset RCTW-17, Mask TTD outperforms other methods by a large margin in terms of both the Average Precision and F-measure, showing its powerful generalization ability.

10.
J Immunol Res ; 2019: 7478538, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31781681

RESUMO

It has been proposed that mutant p53 is correlated with the recurrence of lung cancer. Recently, a small population of cells with asymmetric or symmetric self-renewal potential has been identified in lung cancer, which was termed as cancer stem-like cells (CSCs) and was speculated to be the reason for cancer recurrence after chemotherapy. In this study, we used lung cancer cell lines with different TP53 backgrounds to elucidate the potential role of mutant p53 in regulating lung CSC self-renewal and on lung cancer recurrence. Cisplatin-resistant lung cancer cells with different TP53 backgrounds were generated in vitro by exposing A549, H460, and H661 lung cancer cell lines repeatedly to cisplatin. CD44+/CD90+ stem-like cells were identified in above cisplatin-resistant lung cancers (termed as cisplatin-resistant lung cancer stem-like cells, (Cr-LCSCs)) and stained with PKH26 dye which was used to define the self-renewal pattern. The proportion of symmetric divisions was significantly higher in Cr-LCSCs with mutant (mt) p53 compared with Cr-LCSCs with wild-type (wt) p53, and forced expression of mt p53 promoted the symmetric division of Cr-LCSCs. Furthermore, fewer macrophages accumulated in subcutaneously implanted xenografts consisting of mt p53 Cr-LCSCs compared with wt p53 Cr-LCSCs. These results indicated that mt p53 might accelerate the recurrence of lung cancer by regulating the self-renewal kinetics of Cr-LCSCs as well as the recruitment of macrophages.


Assuntos
Autorrenovação Celular/genética , Resistencia a Medicamentos Antineoplásicos/genética , Neoplasias Pulmonares/genética , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Células-Tronco Neoplásicas/efeitos dos fármacos , Células-Tronco Neoplásicas/metabolismo , Proteína Supressora de Tumor p53/genética , Animais , Biomarcadores Tumorais , Linhagem Celular Tumoral , Cisplatino/farmacologia , Modelos Animais de Doenças , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Camundongos , Células-Tronco Neoplásicas/patologia , Microambiente Tumoral , Ensaios Antitumorais Modelo de Xenoenxerto
11.
Nat Commun ; 10(1): 4283, 2019 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-31570766

RESUMO

The foodborne pathogen Listeria monocytogenes (Lm) is a highly heterogeneous species and currently comprises of 4 evolutionarily distinct lineages. Here, we characterize isolates from severe ovine listeriosis outbreaks that represent a hybrid sub-lineage of the major lineage II (HSL-II) and serotype 4h. HSL-II isolates are highly virulent and exhibit higher organ colonization capacities than well-characterized hypervirulent strains of Lm in an orogastric mouse infection model. The isolates harbour both the Lm Pathogenicity Island (LIPI)-1 and a truncated LIPI-2 locus, encoding sphingomyelinase (SmcL), a virulence factor required for invasion and bacterial translocation from the gut, and other non-contiguous chromosomal segments from another pathogenic species, L. ivanovii. HSL-II isolates exhibit a unique wall teichoic acid (WTA) structure essential for resistance to antimicrobial peptides, bacterial invasion and virulence. The discovery of isolates harbouring pan-species virulence genes of the genus Listeria warrants global efforts to identify further hypervirulent lineages of Lm.


Assuntos
Listeria monocytogenes/genética , Listeriose/microbiologia , Animais , Células CACO-2 , Genoma Bacteriano , Genômica , Cabras/microbiologia , Humanos , Listeria monocytogenes/isolamento & purificação , Listeria monocytogenes/patogenicidade , Camundongos , Filogenia , Suínos/microbiologia , Virulência
12.
Epilepsy Behav ; 98(Pt A): 201-206, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31382177

RESUMO

OBJECTIVE: We have previously shown that cannabidiol (CBD; Epidiolex®) significantly affects levels of clobazam/N-desmethylclobazam, rufinamide, topiramate, zonisamide, and eslicarbazepine. In the present study, we tested whether the presence of concomitant clobazam affected seizure frequency and severity (treatment response) 12 weeks after initiation of therapy with CBD in patients with treatment-resistant epilepsy (TRE). The secondary questions were whether the presence of any of the other antiepileptic drugs (AEDs) had an effect on seizure frequency or severity at 12, 24, or 48 weeks after therapy initiation. METHODS: One hundred and thirty-two adults and children with TRE receiving CBD were studied prospectively. Participants were separated into two groups - either taking (CBD + clobazam) or not taking concomitant clobazam (CBD - clobazam). In the secondary analyses, participants were divided into groups depending on whether they were taking at least 1/4 of the other AEDs shown to interact with CBD (iAED). Seizure counts and Chalfont Seizure Severity Scale (CSSS) were obtained at baseline, 12, 24, and 48 weeks. Groups were compared at each respective time point in the study using generalized estimating equations (GEE) analyses. RESULTS: All groups demonstrated statistically significant reductions in seizure frequency and severity from baseline (all P < 0.05). When participants on CBD + clobazam were compared with CBD - clobazam, there were no significant differences in seizure frequency and severity reduction between the groups at 12 weeks (both P > 0.05). When comparing groups with iAEDs vs. group without iAEDs, independent of coadministration of clobazam, no differences in treatment response were observed (all P > 0.05). Longitudinal analyses up to 48 weeks after therapy initiation did not reveal any differences in treatment response between groups. CONCLUSION: These analyses suggest that concomitant to CBD, AEDs may not have an effect on reducing seizure frequency and severity in patients with TRE.

13.
Diabetes Metab Syndr Obes ; 12: 637-645, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31118725

RESUMO

Background: Using artificial intelligence to assist in diagnosing diseases has become a contemporary research hotspot. Conventional automatic diagnostic method uses a conventional machine learning algorithm to distinguish features from which a professional doctor manually extracts features in diagnostic reports. But it can be difficult to collect large amounts of necessary medical data. Therefore, these methods face challenges with efficiency and accuracy. Method: Here, we proposed an automatic diagnostic system based on a deep learning algorithm to diagnose hyperlipidemia by using human physiological parameters. This model is a neural network which uses technologies of data extension and data correction. Firstly, we corrected and supplemented the original data by the method mentioned previously to solve the problem of lacking data. Secondly, the processed data were used to train a deep learning model. Deep learning model can automatically extract all the available information instead of artificially reducing the raw data. Therefore, it can reduce labor costs. The classifiers classify the data by using features previously mentioned. Finally, the system was evaluated with data from a test dataset. Result: It achieved 91.49% accuracy, 87.50% sensitivity, 93.33% specificity, and 87.50% precision with data from the test dataset. Conclusion: The proposed diagnostic method has a highly robust and accurate performance, and can be used for tentative diagnosis. It can automatically diagnose diseases by using human physiological parameters, thereby reducing labor cost, which results in effective improvement of clinical diagnostic efficiency.

14.
Biochem Biophys Res Commun ; 508(3): 675-681, 2019 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-30527807

RESUMO

Activation of T cell factor-4 (TCF-4) is causally linked to the development of lung carcinoma, while the mechanism of sequence-dependent TCF-4 activity is still obscure. Using reverse transcription-polymerase chain reaction (RT-PCR), here, we demonstrated that sequences of exon 11 in TCF-4 were present in lung carcinoma cells but not in normal lung epithelial cells. Loss of exon 11 in TCF-4 inhibited TCF-4-induced cell growth of lung carcinoma and prolonged the survival time of Lewis lung carcinoma (LLC) tumor-bearing mice. Mechanistically, loss of exon 11 in TCF-4 attenuated the binding activity between TCF-4 protein and its canonical binding site, inhibited TOP/FOP luciferase activity and suppressed mRNA expression of Wnt signaling targets. By performing truncated and site-directed mutations, we further demonstrated that the 16th amino acid serine in exon 11 was responsible for TCF-4-mediated Wnt signaling. In vivo experiments indicated that a mutation of the 16th amino acid serine in exon 11 of TCF-4 could mimic the anti-tumor effect of Wnt signaling inhibitor. Taken together, we identified a serine determining the transcriptional activity of TCF-4 in lung carcinoma cells, and sequencing of TCF-4 mRNA might be an effective strategy to evaluate the Wnt pathway activation and prognosis in lung cancer.


Assuntos
Éxons/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Pulmonares/genética , Serina/metabolismo , Proteína 2 Semelhante ao Fator 7 de Transcrição/genética , Transcrição Genética , Animais , Sequência de Bases , Linhagem Celular Tumoral , Proliferação de Células , Humanos , Camundongos Endogâmicos C57BL , Relação Estrutura-Atividade , Análise de Sobrevida , Proteína 2 Semelhante ao Fator 7 de Transcrição/metabolismo , Via de Sinalização Wnt
15.
J Thorac Dis ; 10(10): 5673-5684, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30505475

RESUMO

Background: The aim of this study was to investigate differences in the imaging features of mass-like tuberculosis and lung cancer on conventional MR sequences to improve the diagnostic ability for pulmonary masses. Methods: Thirty patients with suspicious pulmonary lesions were enrolled and diagnosed with tuberculosis by pathology or comprehensive clinical diagnoses. Twenty-six cases of lung cancer were retrospectively analyzed. Transverse fat-suppressed T2-weighted (T2W) imaging and T1-weighted (T1W) imaging were obtained at 1.5 Tesla. The imaging characteristics of lesions on the T2W and T1W images were compared between the two groups. The imaging features of enlarged mediastinal lymph nodes on T2W images were studied and compared. Results: On T2W images, there was a higher percentage of lesions containing hypointensity in the tuberculosis group (GTB) than in the lung cancer group (GLC) (P=0.004).The incidence of lesions demonstrating heterogeneous intensity was significantly greater in the GTB than in the GLC (70.0% vs. 7.7%, P=0.001). Approximately 92.3% of the lung cancer cases showed hyperintensity, a proportion substantially greater than that in the GTB (6.7%). On T1W images, more cases showed hyperintensity in the GTB than in the GLC (43.3% vs. 7.7%, P=0.003). The signal intensity ratios (SIRs) of the lesion to rhomboid muscle on T2W and T1W images were significantly different between the two groups. The mean intrasubject standard deviation (SD) of lesions in the GTB was markedly greater than that in the GLC on both T2W and T1W images. Benign mediastinal lymph nodes in the GTB showed a variety of signals on T2W images, whereas 80% of metastatic mediastinal lymph nodes displayed slight homogeneous hyperintensity, and this difference between the two groups was statistically significant. Conclusions: Conventional MR sequences can reveal the essential differences between mass-like tuberculosis and lung cancer and may be helpful for discriminating pulmonary masses.

16.
Nanomicro Lett ; 10(1): 17, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30393666

RESUMO

To improve the visible light absorption and photocatalytic activity of titanium dioxide nanotube arrays (TONTAs), ZnFe2O4 (ZFO) nanocrystals were perfused into pristine TONTA pipelines using a novel bias voltage-assisted perfusion method. ZFO nanocrystals were well anchored on the inner walls of the pristine TONTAs when the ZFO suspensions (0.025 mg mL-1) were kept under a 60 V bias voltage for 1 h. After annealing at 750 °C for 2 h, the heterostructured ZFO/Fe2TiO5 (FTO)/TiO2 composite nanotube arrays were successfully obtained. Furthermore, Fe3+ was reduced to Fe2+ when solid solution reactions occurred at the interface of ZFO and the pristine TONTAs. Introducing ZFO significantly enhanced the visible light absorption of the ZFO/FTO/TONTAs relative to that of the annealed TONTAs. The coexistence of type I and staggered type II band alignment in the ZFO/FTO/TONTAs facilitated the separation of photogenerated electrons and holes, thereby improving the efficiency of the ZFO/FTO/TONTAs for photocatalytic degradation of methylene blue when irradiated with simulated sunlight.

17.
Anim Reprod Sci ; 198: 1-10, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30266523

RESUMO

Embryonic diapause occurs in over 130 species of mammals. In embryonic diapause, there is a reversible cessation phase in embryo development and subsequent reactivation and continuation of development with no adverse effects. This allows animals to prolong gestation and give birth to offspring at a more favorable time from a survival perspective. Embryonic diapause is divided into two types: facultative diapause which is induced by physiological conditions and obligate diapause which is induced by season of the year. The process of embryonic diapause includes three phases: onset, maintenance, and reactivation. With facultative diapause the suckling stimulus promotes secretion of prolactin, which suppresses development and secretion activity of the corpus luteum (CL). The decrease of progesterone, secreted by CL, subsequently results in embryos in the diapause state. The embryonic diapause that occurs in mink is of the obligate type and is regulated by photoperiod. The short daylight (<12 h) before the vernal equinox induces an increase in the release of melatonin from the pineal gland. The secretion of prolactin is suppressed and the production of progesterone from CL is reduced. During these phases, many others factors that regulate embryonic and uterine status, hormonal profile, polyamines, and uterine secretion, have been monitored. In recent times, the understanding of the role of different molecular events in embryonic diapause has been advanced. The present review summarizes the research advances related to embryonic diapause to enhance the understanding of this phenomenon and to be considered when there are future research endeavors related to embryonic diapause.


Assuntos
Diapausa/fisiologia , Desenvolvimento Embrionário/fisiologia , Mamíferos/embriologia , Animais , Embrião de Mamíferos , Feminino , Humanos , Gravidez
18.
Epilepsy Behav ; 87: 131-136, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30100226

RESUMO

The objective of this study was to characterize the changes in adverse events, seizure severity, and frequency in response to a pharmaceutical formulation of highly purified cannabidiol (CBD; Epidiolex®) in a large, prospective, single-center, open-label study. We initiated CBD in 72 children and 60 adults with treatment-resistant epilepsy (TRE) at 5 mg/kg/day and titrated it up to a maximum dosage of 50 mg/kg/day. At each visit, we monitored treatment adverse events with the adverse events profile (AEP), seizure severity using the Chalfont Seizure Severity Scale (CSSS), and seizure frequency (SF) using seizure calendars. We analyzed data for the enrollment and visits at 12, 24, and 48 weeks. We recorded AEP, CSSS, and SF at each follow-up visit for the weeks preceding the visit (seizures were averaged over 2-week periods). Of the 139 study participants in this ongoing study, at the time of analysis, 132 had 12-week, 88 had 24-week, and 61 had 48-week data. Study retention was 77% at one year. There were no significant differences between participants who contributed all 4 data points and those who contributed 2 or 3 data points in baseline demographic and AEP/SF/CSSS measures. For all participants, AEP decreased between CBD initiation and the 12-week visit (40.8 vs. 33.2; p < 0.0001) with stable AEP scores thereafter (all p ≥ 0.14). Chalfont Seizure Severity Scale scores were 80.7 at baseline, decreasing to 39.2 at 12 weeks (p < 0.0001) and stable CSSS thereafter (all p ≥ 0.19). Bi-weekly SF decreased from a mean of 144.4 at entry to 52.2 at 12 weeks (p = 0.01) and remained stable thereafter (all p ≥ 0.65). Analyses of the pediatric and adult subgroups revealed similar patterns. Most patients were treated with dosages of CBD between 20 and 30 mg/kg/day. For the first time, this prospective, open-label safety study of CBD in TRE provides evidence for significant improvements in AEP, CSSS, and SF at 12 weeks that are sustained over the 48-week duration of treatment.


Assuntos
Anticonvulsivantes/uso terapêutico , Canabidiol/uso terapêutico , Convulsões/diagnóstico , Convulsões/tratamento farmacológico , Índice de Gravidade de Doença , Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Estudos Prospectivos , Adulto Jovem
19.
Cell Physiol Biochem ; 46(3): 1065-1077, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29669315

RESUMO

BACKGROUND/AIMS: Giant pandas, an endangered species, are a powerful symbol of species conservation. Giant pandas may suffer from a variety of diseases. Owing to their highly specialized diet of bamboo, giant pandas are thought to have a relatively weak ability to resist diseases. The spleen is the largest organ in the lymphatic system. However, there is little known about giant panda spleen at a molecular level. Thus, clarifying the regulatory mechanisms of spleen could help us further understand the immune system of the giant panda as well as its conservation. METHODS: The two giant panda spleens were from two male individuals, one newborn and one an adult, in a non-pathological condition. The whole transcriptomes of mRNA, lncRNA, miRNA, and circRNA in the two spleens were sequenced using the Illumina HiSeq platform. EBseq and IDEG6 were used to observe the differentially expressed genes (DEGs) between these two spleens. Gene Ontology and KEGG analyses were used to annotate the function of DEGs. Furthermore, networks between non-coding RNAs and protein-coding genes were constructed to investigate the relationship between non-coding RNAs and immune-associated genes. RESULTS: By comparative analysis of the whole transcriptomes of these two spleens, we found that one of the major roles of lncRNAs could be involved in the regulation of immune responses of giant panda spleens. In addition, our results also revealed that microRNAs and circRNAs may have evolved to regulate a large set of biological processes of giant panda spleens, and circRNAs may function as miRNA sponges. CONCLUSION: To our knowledge, this is the first report of lncRNAs and circRNAs in giant panda, which could be a useful resource for further giant panda research. Our study reveals the potential functional roles of miRNAs, lncRNAs, and circRNAs in giant panda spleen.


Assuntos
Sistema Imunitário/metabolismo , RNA Longo não Codificante/metabolismo , Baço/metabolismo , Transcriptoma , Ursidae/genética , Animais , MicroRNAs/metabolismo , RNA/química , RNA/isolamento & purificação , RNA/metabolismo , RNA Mensageiro/metabolismo , Análise de Sequência de RNA , Ursidae/metabolismo
20.
J Hazard Mater ; 352: 121-129, 2018 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-29602071

RESUMO

The ability of bentonite and montmorillonite pillared by Al-oxide and mixed (Ln-Al)-oxides (Ln = La, Ce) to remove 239plutonium solution species from water is comparatively investigated at pH 7 and pH 4. Small-angle scattering and neutron contrast variation with H2O/D2O mixtures is used to verify the ingress of water in the calcined products after hydrophilicity was introduced by an NH3-H2O vapor treatment. The size and shape of the (La/Ce)-Al oxo-hydroxy pillaring cations (2 nm spheres) is determined by small-angle x-ray scattering from the pillaring solutions. Not all of the oxide pillars improved Pu uptake compared with sodium montmorillonite. At neutral and acidic pH only (Ce-Al)-oxide pillared clays showed the ability to remove Pu over the concentration range studied (1.35 × 10-8-8 × 10-8 mol dm-3) with distribution coefficient (KD) values >104. XPS analysis of the (Ce-Al)-oxide pillared clays indicates the presence of Ce4+ as cerium dioxide. The progressive improvement in sorption performance in the order of pillar type Al2O3 < La2O3-Al2O3 << CeO2-Al2O3 reflects the increasing access of Pu solution species to the clay mineral layers by changes to the basal spacing and specific surface area, and also to the higher stability of the (Ce-Al)-oxide pillars.

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