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1.
Cell Death Dis ; 11(2): 81, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32015336

RESUMO

Glioblastoma is the most common and malignant form of primary central nervous tumor in adults. Long noncoding RNAs (lncRNAs) have been reported to play a pivotal role in modulating gene expression and regulating human tumor's malignant behaviors. In this study, we confirmed that lncRNA brain-derived neurotrophic factor antisense (BDNF-AS) was downregulated in glioblastoma tissues and cells, interacted and stabilized by polyadenylate-binding protein cytoplasmic 1 (PABPC1). Overexpression of BDNF-AS inhibited the proliferation, migration, and invasion, as well as induced the apoptosis of glioblastoma cells. In the in vivo study, PABPC1 overexpression combined with BDNF-AS overexpression produced the smallest tumor and the longest survival. Moreover, BDNF-AS could elicit retina and anterior neural fold homeobox 2 (RAX2) mRNA decay through STAU1-mediated decay (SMD), and thereby regulated the malignant behaviors glioblastoma cells. Knockdown of RAX2 produced tumor-suppressive function in glioblastoma cells and increased the expression of discs large homolog 5 (DLG5), leading to the activation of the Hippo pathway. In general, this study elucidated that the PABPC1-BDNF-AS-RAX2-DLG5 mechanism may contribute to the anticancer potential of glioma cells and may provide potential therapeutic targets for human glioma.

2.
J Med Virol ; 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-32052466

RESUMO

An outbreak of a novel coronavirus (COVID-19 or 2019-CoV) infection has posed significant threats to international health and the economy. In the absence of treatment for this virus, there is an urgent need to find alternative methods to control the spread of disease. Here, we have conducted an online search for all treatment options related to coronavirus infections as well as some RNA virus infection and we have found that general treatments, coronavirus-specific treatments, and antiviral treatments should be useful in fighting COVID-19. We suggest that the nutritional status of each infected patient should be evaluated before the administration of general treatments and the current children's RNA virus vaccines including influenza vaccine should be immunized for uninfected people and health care workers. In addition, convalescent plasma should be given to COVID-19 patients if it is available. In conclusion, we suggest that all the potential interventions be implemented to control the emerging COVID-19 if the infection is uncontrollable. This article is protected by copyright. All rights reserved.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31985404

RESUMO

OBJECTIVE: This paper aims to propose a 3D laparoscopic imaging system that can realize dense 3D reconstruction in real time. METHODS: Based on the active stereo technique which yields high-density, accurate and robust 3D reconstruction by combining structured light and stereo vision, we design a laparoscopic system consisting of two image feedback channels and one pattern projection channel. Remote high-speed image acquisition and pattern generation lay the foundation for the real-time dense 3D surface reconstruction and enable the miniaturization of the laparoscopic probe. To enhance the reconstruction efficiency and accuracy, we propose a novel active stereo method by which the dense 3D point cloud is obtained using only five patterns, while most existing multiple-shot structured light techniques require 10-40 patterns. In our method, dual-frequency phase-shifting fringes are utilized to uniquely encode the pixels of the measured targets, and a dual-codeword matching scheme is developed to simplify the matching procedure and achieve high-precision reconstruction. RESULTS: Compared with the existing structured light techniques, the proposed method shows better real-time efficiency and accuracy in both quantitative and qualitative ways. Ex-vivo experiments demonstrate the robustness of the proposed method to different biological organs and the effectiveness to lesions and deformations of the organs. Feasibility of the proposed system for real-time dense 3D reconstruction is verified in dynamic experiments. According to the experimental results, the system acquires 3D point clouds with a speed of 12 frames per second. Each frame contains more than 40,000 points, and the average errors tested on standard objects are less than 0.2 mm. SIGNIFICANCE: This paper provides a new real-time dense 3D reconstruction method for 3D laparoscopic imaging. The established prototype system has shown good performance in reconstructing surface of biological tissues.

4.
Cancer Sci ; 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31943575

RESUMO

Upstream ORF (uORF) is a translational initiation element located in the 5'UTR of eukaryotic mRNAs. Studies have found that uORFs play an important regulatory role in many diseases. Based on The Cancer Genome Atlas database, the results of our experiments and previous research evidence, we investigated transcription factor AP-4 (TFAP4) and its uORF, LIM and SH3 protein 1 (LASP1), long noncoding RNA 00520 (LINC00520), and microRNA (miR)-520f-3p as candidates involved in glioma malignancy, which is a poorly understood process. Both TFAP4-66aa-uORF and miR-520f-3p were downregulated, and TFAP4, LASP1, and LINC00520 were highly expressed in glioma tissues and cells. TFAP4-66aa-uORF or miR-520f-3p overexpression or TFAP4, LASP1, or LINC00520 knockdown inhibited glioma cell proliferation, migration, and invasion, but promoted apoptosis. TFAP4-66aa-uORF inhibited the translation of TFAP4 by binding to the TFAP4 mRNA. MicroRNA-520f-3p inhibited TFAP4 expression by binding to its 3'UTR. However, LINC00520 could promote the expression of TFAP4 by competitively binding to miR-520f-3p. In addition, TFAP4 transcriptionally activated LASP1 and LINC00520 expression by binding to their promoter regions, forming a positive feedback loop of TFAP4/LINC00520/miR-520f-3p. Our findings together indicated that TFAP4-66aa-uORF inhibited the TFAP4/LINC00520/miR-520f-3p feedback loop by directly inhibiting TFAP4 expression, subsequently leading to inhibition of glioma malignancy. This provides a basis for developing new therapeutic approaches for glioma treatment.

5.
J Cell Mol Med ; 24(1): 342-355, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31654502

RESUMO

Recent studies indicate circular RNAs are related to dysregulation of vascular endothelial cell function, yet the underlying mechanisms have remained elusive. Here, we characterized the functional role of circular RNA USP1 (circ-USP1) in the regulation of the blood-tumour barrier (BTB) permeability and the potential mechanisms. In the current study, the circ-USP1 expressing level was up-regulated in glioma cerebral microvascular endothelial cells (GECs) of the BTB model in vitro. Knockdown of circ-USP1 disrupted the barrier integrity, increased its permeability as well as reduced tight junction-related protein claudin-5, occludin and ZO-1 expressions in GECs. Bioinformatic prediction and luciferase assay indicated that circ-USP1 bound to miR-194-5p and suppressed its activity. MiR-194-5p contributed to circ-USP1 knockdown-induced increase of BTB permeability via targeting and down-regulating transcription factor FLI1. Furthermore, FLI1 regulated the expressions of claudin-5, occludin and ZO-1 in GECs through binding to their promoter regions. Single or combined treatment of circ-USP1 and miR-194-5p effectively promoted anti-tumour drug doxorubicin across BTB to induce apoptosis of glioma cells. Overall, this present study identified the crucial regulation of circ-USP1 on BTB permeability via miR-194-5p/FLI1 axis-mediated regulation of tight junction proteins, which might facilitate the development of therapeutics against human gliomas.

6.
Comput Methods Programs Biomed ; 183: 105091, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31590098

RESUMO

BACKGROUND AND OBJECTIVE: The functional regions clustering through microelectrode recording (MER) is a critical step in deep brain stimulation (DBS) surgery. The localization of the optimal target highly relies on the neurosurgeon's empirical assessment of the neurophysiological signal. This work presents an unsupervised clustering algorithm to get the optimal cluster result of the functional regions along the electrode trajectory. METHODS: The dataset consists of the MERs obtained from the routine bilateral DBS for PD patients. Several features have been extracted from MER and divided into groups based on the type of neurophysiological signal. We selected single feature groups rather than all features as the input samples of each division of the divisive hierarchical clustering (DHC) algorithm. And the optimal cluster result has been achieved through a feature group combination selection (FGS) method based on genetic algorithm (GA). To measure the performance of this method, we compared the accuracy and validation indexes of three methods, including DHC only, DHC with GA-based FGS and DHC with GA-based feature selection (FS) in other studies, on the universal and DBS datasets. RESULTS: Results show that the DHC with GA-based FGS achieved the optimal cluster result compared with other methods. The three borders of the STN can be identified from the cluster result. The dorsoventral sizes of the STN and dorsal STN are 4.45 mm and 2.02 mm. In addition, the features extracted from the multiunit activity, background unit activity and local field potential are found to be the most representative feature groups to identify the dorsal, d-v and ventral borders of the STN, respectively. CONCLUSIONS: Our clustering algorithm showed a reliable performance of the automatic identification of functional regions in DBS. The findings can provide valuable assistance for both neurosurgeons and stereotactic surgical robots in DBS surgery.

7.
Mol Ther Nucleic Acids ; 18: 1072-1090, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31791014

RESUMO

The blood-tumor barrier (BTB) limits the transport of chemotherapeutic drugs to brain tumor tissues and impacts the treatment of glioma. Long non-coding RNAs play critical roles in various biological processes of tumors; however, the function of these in BTB permeability is still unclear. In this study, we have identified that long intergenic non-protein coding RNA 174 (linc00174) was upregulated in glioma endothelial cells (GECs) from glioma tissues. Additionally, linc00174 was also upregulated in GECs from the BTB model in vitro. Knock down of linc00174 increased BTB permeability and reduced the expression of the tight junction-related proteins ZO-1, occludin, and claudin-5. Both bioinformatics data and results of luciferase reporter assays demonstrated that linc00174 regulated BTB permeability by binding to miR-138-5p and miR-150-5p. Furthermore, knock down of linc00174 inhibited FOSL2 expression via upregulating miR-138-5p and miR-150-5p. FOSL2 interacted with the promoter regions and upregulated the promoter activity of ZO-1, occludin, claudin-5, and linc00174 in GECs. In conclusion, the present study demonstrated that the linc00174/miR-138-5p (miR-150-5p)/FOSL2 feedback loop played an essential role in regulating BTB permeability.

8.
Mol Ther ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31813799

RESUMO

Studies have found that RNA-binding proteins (RBPs) and long non-coding RNAs (lncRNAs) are dysregulated and play an important regulatory role in the development of tumors. Based on The Cancer Genome Atlas (TCGA) database, our findings from experiments, and the evidence of previous studies, we screened DiGeorge syndrome critical region gene 8 (DGCR8), ZFAT antisense RNA 1 (ZFAT-AS1), and caudal type homeobox 2 (CDX2) as research candidates. In the present study, DGCR8 and CDX2 were highly expressed and ZFAT-AS1 was markedly downregulated in glioma tissues and cells. DGCR8 or CDX2 knockdown or ZFAT-AS1 overexpression suppressed glioma cell proliferation, migration, and invasion and facilitated apoptosis. DGCR8 might decrease ZFAT-AS1 expression by attenuating its stability in a manner of inducing its cleavage. Importantly, ZFAT-AS1 could inhibit CDX2 transcription by mediating the methylation of histone H3 on lysine 27 (H3K27me3) modification induced by PRC2 in the CDX2 promoter region. In addition, CDX2 transcriptionally activated DGCR8 expression by binding to its promoter regions, forming a positive feedback loop of DGCR8/ZFAT-AS1/CDX2. In conclusion, DGCR8/ZFAT-AS1 promotes CDX2 transcription in a PRC2 complex-dependent manner to facilitate the malignant biological behavior of glioma cells.

9.
Cell Death Dis ; 10(12): 960, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31862871

RESUMO

The presence of the blood-tumor barrier (BTB) severely impedes the transport of anti-neoplasm drugs to the central nervous system, affecting the therapeutic effects of glioma. Glioma endothelial cells (GECs) are the main structural basis of the BTB. Circular RNA is considered to be an important regulator of endothelial cell growth. In this study, we found that polypyrimidine tract binding protein 1 (PTBP1) and circRNA_001160 were remarkably upregulated in GECs. Knockdown of PTBP1 or circRNA_001160 significantly increased BTB permeability, respectively. As a molecular sponge of miR-195-5p, circRNA_001160 attenuated its negative regulation of the target gene ETV1 by adsorbing miR-195-5p. In addition, ETV1 was overexpression in GECs. ETV1 bounded to the promoter regions of tight junction-related proteins and increased the promoter activities, which significantly promoted the expression levels of tight junction-related proteins. The present study showed that the combined application of PTBP1, circRNA_001160, and miR-195-5p with the anti-tumor drug Dox effectively promoted Dox through BTB and extremely induced the apoptosis of glioma cells. Our results demonstrated that the PTBP1/circRNA_001160/miR-195-5p/ETV1 axis was critical in the regulation of BTB permeability and provided new targets for the treatment of glioma.

10.
Sensors (Basel) ; 19(19)2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31574973

RESUMO

Map building and map-based relocalization techniques are important for unmanned vehicles operating in urban environments. The existing approaches require expensive high-density laser range finders and suffer from relocalization problems in long-term applications. This study proposes a novel map format called the ClusterMap, on the basis of which an approach to achieving relocalization is developed. The ClusterMap is generated by segmenting the perceived point clouds into different point clusters and filtering out clusters belonging to dynamic objects. A location descriptor associated with each cluster is designed for differentiation. The relocalization in the global map is achieved by matching cluster descriptors between local and global maps. The solution does not require high-density point clouds and high-precision segmentation algorithms. In addition, it prevents the effects of environmental changes on illumination intensity, object appearance, and observation direction. A consistent ClusterMap without any scale problem is built by utilizing a 3D visual-LIDAR simultaneous localization and mapping solution by fusing LIDAR and visual information. Experiments on the KITTI dataset and our mobile vehicle illustrates the effectiveness of the proposed approach.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31494541

RESUMO

Despite successful clinical applications, teleoperated robotic surgical systems face particular limitations in the functional endoscopic sinus surgery (FESS) in terms of incompatible instrument dimensions and robot set-up. The endoscope remains manually handled by an assistant when the surgeon performs bimanual operations. This paper introduces the development of the Foot-controlled Robot-Enabled EnDOscope Manipulator (FREEDOM) designed for FESS. The system features clinical considerations that inform the design for providing reliable and safe endoscope positioning with minimal obstruction to the routine practice. The robot structure is modular and compact to ensure coaxial instrument manipulation through the nostril for manual procedures. To alter rigid endoscope motions, a new compliant endoscope holder is proposed that passively limits the lens-tissue contact force under collision for patient-side protection. To facilitate hands-free endoscope manipulation that imposes minimal distractions to the surgeon, a foot-wearable interface is further designed to relieve the assistant's workload. The foot control method owns a short learning curve (mean 3.4 mins), and leads the task to be more ergonomic and surgeon-centered. Cadaver and clinical studies were both conducted to evaluate the surgical applicability of the FREEDOM to assist endoscope manipulation in FESS. The system was validated to be safe (IEC-60601-1) and easy for set up (mean 3.6 mins), from which the surgeon could perform various three-handed procedures alone in FESS without disrupting the routine practice.

12.
Cancer Gene Ther ; 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31455836

RESUMO

Targeted therapy results in objective responses in cervical cancer. However, the responses are short. In contrast, treatment with immune checkpoint inhibitors results in a lower responses rate, but the responses tend to be more durable. Based on these findings, we hypothesized that HPV16 E6/E7-targeted therapy may synergize with the PD-1 pathway blockade to enhance antitumor activity. To test hypothesis, we described for the first time the effects of the CRISPR/Cas9 that was targeted to the HPV and PD1 in vitro and in vivo. Our data showed that gRNA/cas9 targeted HPV16 E6/E7 induced cervical cancer cell SiHa apoptosis, and suggested that overexpression of PD-L1, induced by HPV16 E6/E7, may be responsible for lymphocyte dysfunction. In established SiHa cell- xenografted humanized SCID mice, Administration of gRNA-PD-1 together with gRNA-HPV16 E6/E7 treatment improved the survival and suppressed the tumor growth obviously. In addition, combination treatment increased the population of dendritic cells, CD8+ and CD4+ T lymphocyte cells. According, it enhanced the expression of Th1-associated immune-stimulating genes while reducing the transcription of regulatory/suppressive immune genes, reshaping tumor microenvironment from an immunosuppressive to a stimulatory state. These results demonstrate potent synergistic effects of combination therapy using HPV16 E6/E7-targeted therapy and immune checkpoint blockade PD1, supporting a direct translation of this combination strategy in clinic for the treatment of cervical cancer.

13.
Cell Death Dis ; 10(9): 632, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31434870

RESUMO

Following the publication of this article, the authors realized there was an error in Figure 6H in which two versions of the figure appear.This does not impact the conclusions of the article.

14.
Cell Death Dis ; 10(9): 629, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31427569

RESUMO

There is growing evidence that the long non-coding RNAs(lncRNAs) play an important role in the biological behaviors of glioblastoma cells. In this study, we elucidated the function and possible effect and molecular mechanisms of lncRNA-Linc-00313 on the biological behaviors of glioblastoma cells as well as UPF1 function as a RNA-binding protein to enhance its stability. Here, we used qRT-PCR and western blot to measure the expression, cell Transfection to disrupt the expression of genes, cell viability analysis, quantization of apoptosis, cell migration, and invasion assays, Reporter vectors construction and luciferase assays to investigate the malignant biological behaviors of cells, human lncRNA microarrays, RNA-Immunoprecipitation, dual-luciferase gene reporter assay, half-life assay and chromatin immunoprecipitation to verify the binding sites, tumor xenograft implantation for in vivo experiment, SPSS 18.0 statistical software for data statistics. UPF1 and Linc-00313 were both upregulated in glioma tissues and cells. Knockdown of UPF1 or Linc-00313 significantly inhibited malignant biological behaviors of glioma cells by regulating miR-342-3p and miR-485-5p, which are downregulated and functioned as tumor suppressors in glioma. Furthermore, Linc-00313 could acted as a competing endogenous RNA(ceRNA) to regulate the expression of Zic4 by binding to miR-342-3p and miR-485-5p. Interestingly, Zic4 could bind to the promoters of UPF1 and Linc-00313 respectively and upregulate the expression of them. These results indicated that a positive-feedback loop was formed in the regulation of the biological behaviors of glioma cells. The study is the first to prove that the UPF1-Linc-00313-miR-342-3p/miR-485-5p-Zic4-SHCBP1 pathway forms a positive-feedback loop and regulates the biological behaviors of U87 and U251 cells, which might provide a new therapeutic target for glioma.

15.
Artigo em Inglês | MEDLINE | ID: mdl-31295976

RESUMO

Objective: To investigate the efficiency of diffusion tensor imaging (DTI) fiber-tracking based neuronavigation and assess its usefulness in the preoperative surgical planning, prognostic prediction, intraoperative course and outcome improvement. Methods: Seventeen patients with cerebral masses adjacent to corticospinal tract (CST) were given standard magnetic resonance imaging and DTI examination. By incorporation of DTI data, the relation between tumor and adjacent white matter tracts was reconstructed and assessed in the neuronavigation system. Distance from tumor border to CST was measured. Results: The sub-portion of CST in closest proximity to tumor was found displaced in all patients. The chief disruptive changes were classified as follows : complete interruption, partial interruption, or simple displacement. Partial interruption was evident in seven patients (41.2%) whose lesions were close to cortex. In the other 10 patients (58.8%), delineated CSTs were intact but distorted. No complete CST interruption was identified. Overall, the mean distance from resection border to CST was 6.12 mm (range, 0-21), as opposed to 8.18 mm (range, 2-21) with simple displacement and 2.33 mm (range, 0-5) with partial interruption. The clinical outcomes were analyzed in groups stratified by intervening distances (close, <5 mm; moderated, 5-10 mm; far, >10 mm). For the primary brain tumor patients, the proportion of completely resected tumors increased progressively from close to far grouping (42.9%, 50%, and 100%, respectively). Five patients out of seven (71.4%) experienced new neurologic deficits postoperatively in the close group. At meantime, motor deterioration was found in six cases in the close group. All patients in the far and moderate groups received excellent (modified Rankin Scale [mRS] score, 0-1) or good (mRS score, 2-3) rankings, but only 57.1% of patients in the close group earned good outcome scores. Conclusion: DTI fiber tracking based neuronavigation has merit in assessing the relation between lesions and adjacent white matter tracts, allowing prediction of patient outcomes based on lesion-CST distance. It has also proven beneficial in formulating surgical strategies.

16.
Cell Death Dis ; 10(7): 536, 2019 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-31296839

RESUMO

The existence of blood-tumor barrier (BTB) severely restricts the efficient delivery of antitumor drugs to cranial glioma tissues. Various strategies have been explored to increase BTB permeability. RNA-binding proteins and circular RNAs have recently emerged as potential regulators of endothelial cells functions. In this study, RNA-binding protein KH RNA-binding domain containing, signal transduction associated 3 (KHDRBS3) and circular RNA DENND4C (cDENND4C) were enriched in GECs. KHDRBS3 bound to cDENND4C and increased its stability. The knockdown of cDENND4C increased the permeability of BTB via downregulating the expressions of tight junction-related proteins. The miR-577 was lower expressed in GECs. The overexpressed miR-577 increased the permeability of BTB by reducing the tight junction-related protein expressions, and vice versa. Furthermore, cDENND4C acted as a molecular sponge of miR-577, which bound to miR-577 and inhibited its negative regulation of target genes ZO-1, occludin and claudin-1 to regulate BTB permeability. Single or combined treatment of KHDRBS3, cDENND4C, and miR-577 effectively promoted antitumor drug doxorubicin (DOX) across BTB to induce apoptosis of glioma cells. Collectively, the present study indicated that KHDRBS3 could regulate BTB permeability through the cDENND4C/miR-577 axis, which enhanced doxorubicin delivery across BTB. These findings may provide a novel strategy for chemotherapy of brain tumors.

17.
Sci Total Environ ; 689: 516-525, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31279198

RESUMO

Urbanization is one of the main causes of land use change, especially from 1990 to now in China, but knowledge of its effect on different functional groups of carabids and spiders in the adjacent rural areas over time remains limited. We assessed whether landscape alterations (1993 versus 2013) drove changes in carabid and spider functional groups (1995 versus 2013) in an agricultural landscape located on the fringe of a rapidly growing city in China. Although built-up land increased from 6.3% to 32% across the whole landscape, the overall species richness of carabids and spiders did not decline. In contrast to the reduction in species richness of large carabids, the species richness of small carabids increased. Species richness of both large and small spiders increased. The species composition of carabids and spiders significantly changed between 1995 and 2013. Species compositions of large, predatory carabids and large or ground-hunting spiders were more sensitive to the changes in built-up land than those of small, omnivorous carabids and small or web-building spiders. The amount of grassland (abandoned land covered by wild grass) also increased as farmers began to work in the city. The increased grassland significantly contributed to the increased species richness of predatory and macropterous carabids. However, increased landscape diversity did not affect species richness of either carabids or spiders. High landscape diversity was related to reduction in field size, resulting in a decrease in the mean body size of carabids. This indicates that evaluating the effect of landscape change on carabid and spider diversity should be based on their functional traits. Different taxa, even different functional groups, have different responses to landscape change. The increase in built-up land did not immediately reduce species richness at the urban fringe. Increasing wild grasslands and combining smaller fields may benefit farmland biodiversity in this region.


Assuntos
Biodiversidade , Besouros/fisiologia , Ecossistema , Aranhas/fisiologia , Animais , China , Cidades , Urbanização
18.
J Neural Eng ; 16(6): 066015, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31266003

RESUMO

OBJECTIVE: The identification of functional regions, in particular the subthalamic nucleus, through microelectrode recording (MER) is the key step in deep brain stimulation (DBS). To eliminate variability in a neurosurgeon's judgment, this study presents an online identification method for identifying functional regions along the electrode trajectory. APPROACH: Functional regions can be identified through offline clustering and online identification based on the unsupervised random forest (RF) algorithm. We took 106 features from MER and the estimated anatomical distance to target as the dataset to train the RF model. To improve the prediction performance and reduce the computation time, a wrapper feature selection (FS) method was added into the algorithm. The method contains feature ranking based on out-of-bag error or silhouette index and feature subset search based on the roulette selection algorithm. MAIN RESULTS: To evaluate the optimization effect of the FS method on the unsupervised RF algorithm, we compared the results of the algorithm with or without FS on the DBS dataset. In addition, the optimization effect of FS on the computation time is evaluated. The results show that for offline clustering, the accuracy obtained with the selected features is higher than that obtained with all features, and the running time decreased from 259.7 s to 60.8 s in the iteration of the FS. The accuracy in online identification improved from 76.19% to 92.08% through FS. In addition, the functional region online identification time is 41.5 ms, which can meet the requirements of DBS surgery. SIGNIFICANCE: In conclusion, using the FS method can improve the accuracy and reduce the computation time of the online identification of functional regions. In addition, the online identification method can provide valuable assistance for both neurosurgeons and stereotactic surgery robots in guiding implantation of the electrode in real time.

19.
Eur Neurol ; 81(3-4): 152-162, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31269499

RESUMO

OBJECTIVE: This study investigated whether pre-operative interictal discharge patterns detected by electroencephalogram (EEG) and magnetic resonance imaging (MRI) findings affect the surgical prognosis in temporal lobe epilepsy (TLE) patients. METHODS: A retrospective analysis of 115 cases of patients with refractory TLE was carried out from October 2010 to 2014 based on the classification of pre-operative interictal discharge patterns in EEG and MRI findings. The patients were followed up for 4 years after surgery. The ILAE method was used to assess differences in seizure-free rate among different types of interictal discharge pattern as well as in MRI findings. RESULTS: A total of 115 cases were classified according to interictal discharge patterns in EEG, including normal cases, unilateral anterior discharge, unilateral multi-region discharge, and bilateral discharge. MRI findings were classified into negative results and positive results. Unilateral anterior and bilateral discharges showed statistically significant differences in post-operative seizure-free rates (p< 0.001). MRI-positive cases showed good overall post-operative outcome, irrespective of interictal discharge pattern in the EEG, whereas MRI-negative cases showed good overall prognosis if the interictal discharge pattern in EEG occurred in the unilateral anterior region. CONCLUSION: If the pre-operative interictal discharge pattern in EEG is confined to the unilateral anterior region, prognosis is good. If there are abnormalities in MRI findings, post-operative prognosis is good, regardless of pre-operative interictal discharge patterns in EEG. Surgical intervention is highly recommended for TLE patients with normal MRI findings and interictal discharge confined to the unilateral anterior region.

20.
J Cancer ; 10(13): 2961-2968, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31281473

RESUMO

LncRNAs have been proved to be involved in the promotion of glioma cell malignant development. However, the exact roles and molecular mechanisms of linc01023 in glioma remain blurred. In this study, we confirm linc01023 is up-regulated in glioma tissues and cell lines. In addition, elevated linc01023 expression indicates shorter survival times in patients with glioma. Moreover, loss-of-function studies reveal that restoration of linc01023 restrains glioma cell proliferation, migration and invasion by regulating IGF1R/AKT pathway in vitro and in vivo. Collectively, the study indicates that linc01023 plays an oncogenic role in glioma through activation of IGF1R/AKT signal pathway, and it could be a candidate therapeutic target.

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