Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 134
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Nucl Med ; 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31586001

RESUMO

Fibroblast activation protein (FAP), which promotes tumor growth and progression, is overexpressed in cancer-associated fibroblasts of many human epithelial cancers. Owing to its low expression in normal organs, FAP is an excellent target for theranostics. In this study, we used radionuclides with relatively long half-lives, 64Cu (half-life = 12.7 h) and 225Ac (half-life = 10 days), to label FAP inhibitors (FAPI) in mice with human pancreatic cancer xenografts. Methods: Male nude mice (body weight = 22.5 ± 1.2 g) were subcutaneously injected with human pancreatic cancer cells (PANC-1, n = 12; MIA PaCa-2, n = 8). Tumor xenograft mice were investigated after the intravenous injection of 64Cu-FAPI-04 (7.21 ± 0.46 MBq) by dynamic and delayed PET scans (2.5 h post injection). Static scans 1 h after the injection of 68Ga-FAPI-04 (3.6 ± 1.4 MBq) were also acquired for comparisons using the same cohort of mice (n = 8). Immunohistochemical staining was performed to confirm FAP expression in tumor xenografts using an FAP-alpha antibody. For radioligand therapy, 225Ac-FAPI-04 (34 kBq) was injected into PANC-1 xenograft mice (n = 6). Tumor size was monitored and compared to that of control mice (n = 6). Results: Dynamic imaging of 64Cu-FAPI-04 showed rapid clearance through the kidneys and slow washout from tumors. Delayed PET imaging of 64Cu-FAPI-04 showed mild uptake in tumors and relatively high uptake in the liver and intestine. Accumulation levels in the tumor or normal organs were significantly higher for 64Cu-FAPI-04 than 68Ga-FAPI-04, except in the heart, and excretion in the urine was higher for 68Ga-FAPI-04 than 64Cu-FAPI-04. Immunohistochemical staining revealed abundant FAP expression in the stroma of xenografts. 225Ac-FAPI-04 injection showed significant tumor growth suppression in the PANC-1 xenograft mice compared to the control mice, without a significant change in body weight. Conclusion: This proof of concept study showed that 64Cu-FAPI-04 and 225Ac-FAPI-04 could be used in theranostics for the treatment of FAP-expressing pancreatic cancer. Alpha therapy targeting FAP in the cancer stroma is effective and will contribute to the development of a new treatment strategy.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31606164

RESUMO

OBJECTIVE: Contralateral recurrence in patients with primary spontaneous pneumothorax is approximately 15%. If positive for blebs, the recurrence rate increases to 26%. This study seeks to determine whether simultaneous contralateral video-assisted thoracic surgery blebs excision would effectively lower the contralateral incidence of pneumothorax in patients undergoing surgery for ipsilateral primary spontaneous pneumothorax. METHODS: Between January 2009 and December 2015, 335 patients with primary spontaneous pneumothorax, surgically treated in a single institution, were retrospectively studied. The median follow-up was 75 (50-99) months. All patients received video-assisted thoracic surgery blebectomy/bullectomy with pleural abrasions. They were classified into 3 groups: (1) ipsilateral video-assisted thoracic surgery without contralateral blebs/bullae included 142 patients with ipsilateral primary spontaneous pneumothorax without contralateral blebs/bullae only receiving ipsilateral video-assisted thoracic surgery; (2) ipsilateral video-assisted thoracic surgery with contralateral blebs/bullae included 123 patients with ipsilateral primary spontaneous pneumothorax with contralateral blebs/bullae receiving only ipsilateral video-assisted thoracic surgery; and (3) bilateral video-assisted thoracic surgery with contralateral blebs/bullae included 70 patients with ipsilateral primary spontaneous pneumothorax with contralateral blebs/bullae receiving 1-stage bilateral video-assisted thoracic surgery. Demographic data, perioperative details, recurrence patterns, recurrence-free survivals, and risk factors were compared. RESULTS: The percentage of contralateral recurrence for the ipsilateral video-assisted thoracic surgery without contralateral blebs/bullae, ipsilateral video-assisted thoracic surgery with contralateral blebs/bullae, and bilateral video-assisted thoracic surgery with contralateral blebs/bullae groups differed significantly (0.7%, 14.6%, and 2.9%, respectively; P = .002). Multivariate analysis using the Cox proportional hazard model revealed that age less than 18 years (hazard ratio, 2.71; 95% confidence interval, 1.14-6.44; P = .024) and ipsilateral video-assisted thoracic surgery with contralateral blebs/bullae (hazard ratio, 22.13, 95% confidence interval, 2.96-165, P = .003) were predictors of contralateral recurrence, of which recurrence-free survival was notably different among groups as determined by Kaplan-Meier analysis (P < .0001). CONCLUSIONS: Simultaneous contralateral blebectomy in patients with primary spontaneous pneumothorax receiving ipsilateral video-assisted thoracic surgery significantly lowered future contralateral recurrence.

3.
Neuron ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31585809

RESUMO

In humans, disruption of nonsense-mediated decay (NMD) has been associated with neurodevelopmental disorders (NDDs) such as autism spectrum disorder and intellectual disability. However, the mechanism by which deficient NMD leads to neurodevelopmental dysfunction remains unknown, preventing development of targeted therapies. Here we identified novel protein-coding UPF2 (UP-Frameshift 2) variants in humans with NDD, including speech and language deficits. In parallel, we found that mice lacking Upf2 in the forebrain (Upf2 fb-KO mice) show impaired NMD, memory deficits, abnormal long-term potentiation (LTP), and social and communication deficits. Surprisingly, Upf2 fb-KO mice exhibit elevated expression of immune genes and brain inflammation. More importantly, treatment with two FDA-approved anti-inflammatory drugs reduced brain inflammation, restored LTP and long-term memory, and reversed social and communication deficits. Collectively, our findings indicate that impaired UPF2-dependent NMD leads to neurodevelopmental dysfunction and suggest that anti-inflammatory agents may prove effective for treatment of disorders with impaired NMD.

4.
J Clin Lab Anal ; : e23058, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31617231

RESUMO

BACKGROUND: MicroRNAs (miRNAs) can be used for early diagnosis of myocardial infarction. However, due to a lack of standardized operating procedures, their value for clinical application is low. METHODS: Detection of plasma miRNAs was optimized by analyzing factors influencing miRNA variance and myocardial infarction risk scores during analysis (extraction, reverse transcription, and real-time PCR) and pre-analysis (dietary status, anticoagulants, storage conditions, and hemolysis). RESULTS: Regarding variable factors during analysis, the centrifugal column method was superior to Trizol LS reagent when extracting miRNA from plasma. Recovery rate was highest with plasma volumes of 200 and 300 µL. During analysis, the main source of miRNA detection inaccuracy was derived from RNA extraction (mainly organic extraction), and not reverse transcription or PCR. MiRNA variance could be reduced by use of an internal reference. During analysis, 95% of risk score variation fluctuated within a range of 6.267. The variable factors pre-analysis mainly involved dietary status, anticoagulant selection, and storage conditions. Hemolysis positively correlated with miRNA levels, but there was no significant change in risk score after internal reference calibration. CONCLUSION: Preliminary standardization for miRNA detection provides a reference for clinical blood testing of miRNAs.

5.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 50(4): 604-608, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31642243

RESUMO

OBJECTIVE: To evaluate the clinical effect and rehabilitation benefit of enhanced recovery after surgery (ERAS) model in perioperative management of total hip arthroplasty (THA). METHODS: A retrospective study were conducted in THA patients from the database of big data Research Center of Biomedicine, West China Hospital of Sichuan University from 2013 to 2016. The differences of rehabilitation quality, rehabilitation efficiency and hospitalization cost between ERAS model (ERAS group) and traditional model (traditional group) were compared. RESULTS: 915 THA patients were included in the study, of which 497 patients were given ERAS peri-operative management and 418 patients in the traditional group. The rate of overall complications in the ERAS group was significantly lower than that in the traditional group (4.8% vs. 8.6%, P < 0.05), with lower rate of deep venous thrombosis (0.8% vs. 3.1%) and pulmonary infection (0.6% vs. 2.4%) in the ERAS group. Compared with the traditional group, the average length of stay in hospital was shorter in the ERAS group 〔(7.6±2.0) d vs. (10.9±2.9) d, P=0.039〕, as well as postoperative length of stay in hospital 〔(5.6±0.9) d vs. (8.6±2.0) d, P=0.028)〕. And the ERAS model reduced the total hospitalization cost by 4.8% to 7.1% (P < 0.05), of which the drug cost decreased by 17.2% to 24.9% (P < 0.05). CONCLUSIONS: ERAS model in THA is safe and effective. It can reduce hospitalization cost and help to control medical cost.

6.
Food Funct ; 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31651924

RESUMO

Previous studies have shown that 7S protein is the active ingredient responsible for the plasma cholesterol-lowering activity of soybean. It is hypothesized that isoflavones in soybean could enhance the blood cholesterol-lowering activity of 7S protein. Forty-eight hamsters were divided into six groups and fed a non-cholesterol diet or one of the five high-cholesterol diets containing 12.1% 7S protein with 0-15.62 mg g-1 isoflavones. The results showed that addition of isoflavones in diets dose-dependently enhanced the plasma total cholesterol-lowering activity of 7S protein. Addition of isoflavones in 7S protein-based diets significantly reduced hepatic cholesterol accumulation by 12.6-26.1%, compared with the high cholesterol control diet. Isoflavones could also facilitate excretion of neutral sterols in a dose-dependent manner. Supplementation of isoflavones in diets favourably modulated mRNA expression and the protein mass of HMG-CoA reductase. It was concluded that the enhancing effect of isoflavones on the blood cholesterol-lowering activity of 7S protein was mediated by inhibiting the cholesterol absorption and de novo cholesterol synthesis in hypercholesterolemic hamsters.

7.
Biochem Biophys Res Commun ; 516(4): 1159-1166, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31284955

RESUMO

Liver secretes proliferative factors participating compensatory hyperplasia of islets during obesity and insulin resistance. Extracellular vesicles (EVs) mediate intercellular communication by delivering inner factors to recipient cells. This study explored the biological effects of hepatocellular EVs on islet ß cells during obesity. Compared with standard chow diet (CD), hepatocellular EVs derived from high-fat diet (HFD) induced obese mice promoted proliferation of ß cell line-MIN6 cells, but didn't influence their insulin secretion. Microarray analysis found 13 miRNAs with significantly differential expression in hepatocellular EVs between HFD with CD group. Meanwhile, RNA-sequencing detected 80 genes with significantly differential expression in MIN6 cells treated with HFD and CD hepatocellular EVs respectively. Six miRNAs and 11 potential target genes were pre-screened by synthesizing TargetScan prediction and RNA-sequencing results. After miRNA mimic transfection and testing the expressions of target genes and cell vitality, miR-7218-5p was verified to affect MIN6 cell proliferation through targeting CD74 gene. SiRNA transfection and dual luciferase reporter assay further confirmed the binding and regulation of miRNA-7218-5p on CD74. These findings suggest HFD induced obesity could change miRNA profiles in hepatocellular EVs, which modulate expression of multiple genes and proliferation of MIN6 cells and maybe mediate compensatory hyperplasia of islets.

8.
Biomaterials ; 218: 119336, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31310952

RESUMO

Mesenchymal stem cells (MSCs) embedded in their secreted extracellular matrix (mECM) constitute an exogenous scaffold-free construct capable of generating different types of tissues. Whether MSC-mECM constructs can recapitulate endochondral ossification (ECO), a developmental process during in vivo skeletogenesis, remains unknown. In this study, MSC-mECM constructs are shown to result in robust bone formation both in vitro and in vivo through the process of endochondral ossification when sequentially exposed to chondrogenic and osteogenic cues. Of interest, a novel trypsin pre-treatment was introduced to change cell morphology, which allowed MSC-mECM constructs to undergo the N-cadherin-mediated developmental condensation process and subsequent chondrogenesis. Furthermore, bone formation by MSC-mECM constructs were significantly enhanced by the ECO protocol, as compared to conventional in vitro culture in osteogenic medium alone. This was designed to promote direct bone formation as seen in intramembranous ossification (IMO). The developmentally informed method reported in this study represents a robust and efficacious approach for stem-cell based bone generation, which is superior to the conventional osteogenic induction procedure.

9.
Sci Rep ; 9(1): 9811, 2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31285516

RESUMO

Naphthalene is a biocide of soil fauna, particularly of soil arthropods, that has been widely applied to test the functional roles of soil fauna in soil processes. However, whether the use of naphthalene to expel soil fauna has a non-target effect on soil bacteria in subalpine forests remains unclear. We conducted a naphthalene treatment experiment to explore the effects of naphthalene on the soil bacterial community in subalpine forest soil. The results suggested that naphthalene treatment (at 100 g.m-2 per month) significantly increased the abundances of total bacterial, gram-positive bacterial and gram-negative bacterial phospholipid fatty acids (PLFA) and did not change the microbial biomass carbon (MBC), microbial biomass nitrogen (MBN) or MBC/MBN ratio. Moreover, a total of 1038 operational taxonomic units (OTUs) were detected by Illumina MiSeq sequencing analysis. Proteobacteria, Actinobacteria, and Acidobacteria Chloroflexi were the dominant phyla, and Bradyrhizobium was the most abundant genus. The naphthalene treatment did not affect soil bacterial diversity or community structure. Overall, these results demonstrated that the naphthalene treatment had non-target effects on the active bacterial community abundance but not the soil bacterial community structure. Thus, the non-target effects of naphthalene treatment should be considered before using it to expel soil fauna.

10.
J Med Chem ; 62(13): 6047-6062, 2019 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-31181158

RESUMO

We report that compound 13, a novel phosphatidylserine-targeting zinc(II) dipicolylamine drug conjugate, readily triggers a positive feedback therapeutic loop through the in situ generation of phosphatidylserine in the tumor microenvironment. Linker modifications, pharmacokinetics profiling, in vivo antitumor studies, and micro-Western array of treated-tumor tissues were employed to show that this class of conjugates induced regeneration of apoptotic signals, which facilitated subsequent recruitment of the circulating conjugates through the zinc(II) dipicolylamine-phosphatidylserine association and resulted in compounding antitumor efficacy. Compared to the marketed compound 17, compound 13 not only induced regressions in colorectal and pancreatic tumor models, it also exhibited at least 5-fold enhancement in antitumor efficacy with only 40% of the drug employed during treatment, culminating in a >12.5-fold increase in therapeutic potential. Our study discloses a chemically distinct apoptosis-targeting theranostic, with built-in complementary functional moieties between the targeting module and the drug mechanism to expand the arsenal of antitumor therapy.

11.
Proc Natl Acad Sci U S A ; 116(20): 9741-9746, 2019 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-31010932

RESUMO

Sunlight drives photosynthesis and associated biological processes, and also influences inorganic processes that shape Earth's climate and geochemistry. Bacterial solar-to-chemical energy conversion on this planet evolved to use an intricate intracellular process of phototrophy. However, a natural nonbiological counterpart to phototrophy has yet to be recognized. In this work, we reveal the inherent "phototrophic-like" behavior of vast expanses of natural rock/soil surfaces from deserts, red soils, and karst environments, all of which can drive photon-to-electron conversions. Using scanning electron microscopy, transmission electron microscopy, micro-Raman spectroscopy, and X-ray absorption spectroscopy, Fe and Mn (oxyhydr)oxide-rich coatings were found in rock varnishes, as were Fe (oxyhydr)oxides on red soil surfaces and minute amounts of Mn oxides on karst rock surfaces. By directly fabricating a photoelectric detection device on the thin section of a rock varnish sample, we have recorded an in situ photocurrent micromapping of the coatings, which behave as highly sensitive and stable photoelectric systems. Additional measurements of red soil and powder separated from the outermost surface of karst rocks yielded photocurrents that are also sensitive to irradiation. The prominent solar-responsive capability of the phototrophic-like rocks/soils is ascribed to the semiconducting Fe- and Mn (oxyhydr)oxide-mineral coatings. The native semiconducting Fe/Mn-rich coatings may play a role similar, in part, to photosynthetic systems and thus provide a distinctive driving force for redox (bio)geochemistry on Earth's surfaces.

12.
Nat Commun ; 10(1): 1637, 2019 04 09.
Artigo em Inglês | MEDLINE | ID: mdl-30967542

RESUMO

The competitive endogenous RNA (ceRNA) hypothesis suggests an intrinsic mechanism to regulate biological processes. However, whether the dynamic changes of ceRNAs can modulate miRNA activities remains controversial. Here, we examine the dynamics of ceRNAs during TGF-ß-induced epithelial-to-mesenchymal transition (EMT). We observe that TGFBI, a transcript highly induced during EMT in A549 cells, acts as the ceRNA for miR-21 to modulate EMT. We further identify FN1 as the ceRNA for miR-200c in the canonical SNAIL-ZEB-miR200 circuit in MCF10A cells. Experimental assays and computational simulations demonstrate that the dynamically induced ceRNAs are directly coupled with the canonical double negative feedback loops and are critical to the induction of EMT. These results help to establish the relevance of ceRNA in cancer EMT and suggest that ceRNA is an intrinsic component of the EMT regulatory circuit and may represent a potential target to disrupt EMT during tumorigenesis.


Assuntos
Transição Epitelial-Mesenquimal/genética , Redes Reguladoras de Genes , MicroRNAs/genética , Neoplasias/genética , RNA Mensageiro/genética , Células A549 , Carcinogênese/genética , Biologia Computacional , Proteínas da Matriz Extracelular/genética , Fibronectinas/genética , Regulação Neoplásica da Expressão Gênica , Humanos , MicroRNAs/metabolismo , Modelos Biológicos , RNA Mensageiro/metabolismo , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta/metabolismo
13.
Artigo em Inglês | MEDLINE | ID: mdl-30934095

RESUMO

BACKGROUND: The omission of chest tubes after thoracoscopic procedures such as sympathectomy, lung biopsy, and lung resection has proven efficacious in decreasing pain and length of hospital stay in some cases. However, its safety for mediastinal diseases remains unclear. This study evaluated the feasibility and outcome of eliminating chest drains after video-assisted thoracoscopic surgery (VATS) for mediastinal tumor resection. METHODS: We retrospectively investigated 70 patients receiving VATS mediastinal tumor resection in a single institution between January 2016 and November 2018. A total of 39 patients (drain group) received postoperative chest drains and 31 patients (no-drain group) did not. Group clinical outcomes and operation data were compared. A propensity score matching analysis was further performed to yield a fairer comparison. RESULTS: Before propensity score matching, the no-drain group had a higher prevalence of cystic lesions, a shorter operative time, and less blood loss compared with the drain group (p = 0.015, p = 0.018, and p < 0.001, respectively). After matching, the group differences in these perioperative variables lost significance (p = 0.095, 0.4, and 0.2, respectively). The no-drain group had lower postoperative day 2 pain scores and shorter postoperative hospital stays than the drain group, regardless of whether they were matched (pain: p = 0.028; hospital stay < 0.001) or not (pain: p = 0.003; hospital stay < 0.001). No major adverse events occurred in either group during hospitalization or follow-up period. CONCLUSION: Eliminating chest drain placement after VATS mediastinal tumor resection may benefit some patients and decrease postoperative pain and hospital stay without increasing complications or compromising patient safety.

14.
Artigo em Inglês | MEDLINE | ID: mdl-30909163

RESUMO

The oriental fruit fly, Bactrocera dorsalis, is one of the most destructive pests worldwide. The frequent use of chemical insecticides has led B. dorsalis to develop resistance to many insecticides in recent decades. New high-throughput-sequenced transcriptomes, as well as genomes, have revealed a large number of reference genes for functional target identification. Here, we performed digital gene expression profiling of ovary and testis of B. dorsalis adults. Various genes were identified to be highly expressed in B. dorsalis ovary. The genes encoding components of eggshell, vitelline membrane proteins (Vmps) and chorion-related proteins, were identified to be tissue-specifically expressed in ovary. Five cytochrome P450 genes were also identified to be highly expressed in ovary. Three of them were ecdysone synthesis pathway genes indicating the ovary as a potential synthesis site of female. The up-regulated expression of Vmps by exogenous 20-hydroxyecdysone implied the hormonal regulation of eggshell formation during ovarian development. Many other genes with potential functions in ovarian development were also identified, including vitellogenin receptor, insulin receptor, NASP protein, and odorant binding protein. These findings should promote our understanding of the regulation of vitellogenesis and eggshell formation and enable exploration of potentially novel pest control targets.

15.
Thorac Cancer ; 10(5): 1267-1271, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30861610

RESUMO

This report describes the surgical management of a male patient with early-stage lung cancer who underwent thoracoscopic completion right lower lobectomy after previously undergoing sublobar resection for multifocal ground glass nodules of the lung. Perioperative considerations associated with the management of dense pulmonary hilar adhesions and the techniques used are discussed.

16.
J Nucl Med ; 2019 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-30902876

RESUMO

Purpose PET radioligands with low molar activity (MA) may underestimate the quantity of the target of interest because of competitive binding of the target with unlabeled ligand. The aim of this study was to evaluate the change in the whole-body distribution of 18F-PSMA-1007 targeting prostate specific membrane antigen (PSMA), using solutions with different peptide concentrations. Methods Mouse xenograft models of LNCaP (PSMA-positive prostate cancer) (n = 18) were prepared and divided into three groups according to the peptide concentration injected: high MA group (1,013 ± 146 GBq/µmol; n = 6), medium MA group (100.7 ± 23.1 GBq/µmol; n = 6) and low MA group (10.80 ± 2.84 GBq/µmol; n = 6). Static PET scans were performed one hour after injection (scan duration = 10 min). SUVmean in the tumors and normal organs were compared by the multiple comparison test. Immunohistochemical staining and western blot analysis were performed to confirm expression of PSMA in the tumors, salivary glands and kidneys. Results The low MA group (SUVmean = 1.12 ± 0.30) showed significantly lower uptake of 18F-PSMA-1007 in the tumors as compared to the high MA group (1.97 ± 0.77) and medium MA group (1.81 ± 0.57). On the other hand, in the salivary glands, both the low MA group (SUVmean = 0.24 ± 0.04) and medium MA group (0.57 ± 0.08) showed significantly lower uptakes as compared to the high MA group (1.27 ± 0.28). The tumor-to-salivary gland SUVmean ratio was 1.73 ± 0.55 in high MA, 3.16 ± 0.86 in medium MA, and 4.78 ± 1.29 in low MA. The immunohistochemical staining and western blot analysis revealed significant overexpression of PSMA in the tumors, and low expression in the salivary glands and kidneys. Conclusions Decrease of the MA levels in the injected 18F-PSMA-1007 solutions resulted in decreased uptakes in the tumors as well as the normal salivary glands. With reduction of the MA levels, the salivary glands showed a greater degree decrease of uptake than the tumors. Thus, there is a possibility of minimizing the adverse effects in the salivary glands by setting an appropriate MA levels in PSMA-targeting therapy.

17.
J Invest Surg ; : 1-10, 2019 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-30885012

RESUMO

BACKGROUNDS: Enteral immunonutrition (EIN) has received increasing attention, however, evidence on its immunomodulatory and anti-inflammatory function in gastric cancer patients undergoing gastrectomy is poorly investigated. Objectives of this study were to evaluate the effect of EIN on immune function, inflammation response and nutrition status when compared to standard enteral nutrition (SEN). METHODS: Totally 124 gastric cancer patients after gastrectomy were randomized to receive early 5-days postoperative EIN (formula enriched with arginine, glutamine, omega-3 fatty acids and nucleotide), or SEN. The primary end-points were CD4+ T-cells, CD3+ T-cells as well as counts of CD4+/CD8+, IgG, IgM, and IgA levels. Second-points included white blood cell (WBC), C-reactive protein (CRP), procalcitonin (PCT), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) levels and nutritional index such as serum albumin, prealbumin, and transferrin concentration. RESULTS: There existed significant difference in primary end-points between EIN group and SEN group. The proportion of CD4+ T-cells, CD3+ T-cells and the counts of CD4+/CD8+, IgG, IgM, and IgA were higher in EIN group eventually. Meanwhile, the level of WBC, CRP and TNF-α were significantly lower in EIN group finally. But there were no other significant differences in nutritional markers between two groups. CONCLUSION: Early postoperative EIN significantly improves immune function and inflammatory response in gastric cancer patients undergoing gastrectomy.

18.
J Nucl Med ; 60(9): 1301-1307, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30796173

RESUMO

211At is an α-emitter that has similar chemical properties to iodine and is used in targeted α-therapy. In the present study, we added ascorbic acid (AA) to 211At solution to increase the radiochemical purity of astatide and evaluated its efficacy against differentiated thyroid cancer, which is characterized by the expression of sodium/iodide symporter (NIS). Methods: Crude 211At solution (AA(-)) and 211At solution treated with AA (AA(+)) were prepared. Uptake by the thyroid was compared between the 2 solutions in normal male Wistar rats (n = 6). Cellular uptake in K1-NIS cells was analyzed under the AA(+) and AA(-) conditions. AA(+) was injected at 3 doses into K1-NIS xenograft mice: 1 MBq (n = 6), 0.4 MBq (n = 6), and 0.1 MBq (n = 6), and vehicle was injected into control mice (n = 6). The treatment effects were compared among the 4 groups. Results: Uptake by the thyroid was significantly enhanced in rats injected with the AA(+) as compared with those injected with AA(-). Cellular uptake analysis showed significantly increased uptake of 211At by the K1-NIS cells under the AA(+) condition as compared with the AA(-) condition. In the mouse xenograft model, the K1-NIS tumors showed significant accumulation of 211At at 3 and 24 h after administration (22.5 ± 10.4 and 12.9 ± 6.8 percentage injected dose, respectively). Tumor growth was immediately inhibited in a dose-dependent manner after administration of 211At. In the survival analysis, the 211At groups (0.1, 0.4, and 1 MBq) showed significantly better survival than the control group. Conclusion: Uptake of 211At was enhanced in differentiated thyroid cancer cells as well as the normal thyroid using 211At solution treated with AA. The method also showed dose-dependent efficacy against the K1-NIS xenografts, suggesting its potential applicability to targeted α-therapy.

19.
Sci Rep ; 9(1): 2849, 2019 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-30809005

RESUMO

Naphthalene has been widely used to study the role of soil fauna, but its potential non-target effects on soil enzyme activity remain unknown in subalpine forests. We added naphthalene for two years and determined the effect of such additions on the abundance of soil fauna and soil enzyme activities (ß-glucosidase, cellobiohydrolase, invertase, peroxidase, polyphenol oxidase, N-acetyl-ß-D-glucosaminidase, leucine arylamidase, urease, nitrate reductase and nitrite reductase) in a subalpine forest. Naphthalene could efficiently suppress the individual density and population of soil fauna in situ. The individual density and number of groups were decreased by 72.6-84.8% and 15.0-28.0%, respectively. Naphthalene significantly affected the activities of ß-glucosidase, cellobiohydrolase, polyphenol oxidase, N-acetyl-ß-D-glucosaminidase, leucine arylamidase and nitrite reductase and the activity increased in the first litter peak of naphthalene addition, and decreased at the later. The activities of ß-glucosidase, cellobiohydrolase, polyphenol oxidase, peroxidase, N-acetyl-ß-D-glucosaminidase, leucine arylamidase and nitrite reductase showed a negative correlation with the soil microbial PLFAs. Conversely, the activities of invertase, urease and nitrate reductase were positively correlated with the soil microbial PLFAs. Our results suggest that naphthalene is an effective method to reduce soil fauna in subalpine forest. The enzyme activity was influenced by soil fauna and microbial PLFAs.

20.
Appl Opt ; 58(3): 604-608, 2019 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-30694244

RESUMO

A novel Doppler velocity measurement method based on laser self-mixing interference with two parallel optical external cavities is investigated. In this method, the target object rotation velocity can be measured by the sum of two Doppler frequency shifts, which can be extracted by fast Fourier transform without setting and measuring incident angles. This study highlights the validity of the proposed method through a theoretical model analysis. Experiments realized velocity measurement with a relative error of less than 0.6%.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA