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1.
Integr Comp Biol ; 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32413121

RESUMO

The complexity of an animal's interaction with its physical and/or social environment is thought to be associated with behavioral flexibility and cognitive phenotype, though we know little about this relationship in amphibians. We examined differences in cognitive phenotype in two species of frog with divergent natural histories. The green-and-black poison frog (Dendrobates auratus) is diurnal, displays enduring social interactions, and uses spatially distributed resources during parental care. Túngara frogs (Physalaemus pustulosus) are nocturnal, express only fleeting social interactions, and use ephemeral puddles to breed in a lek-type mating system. Comparing performance in identical discrimination tasks, we find that D. auratus made fewer errors when learning and displayed greater behavioral flexibility in reversal learning tasks than túngara frogs. Further, túngara frogs preferred to learn beacons that can be used in direct guidance whereas D. auratus preferred position cues that could be used to spatially orient relative to the goal. Behavioral flexibility and spatial cognition are associated with hippocampal function in mammals. Accordingly, we examined differential gene expression in the medial pallium, the amphibian homolog of the hippocampus. Our preliminary data indicate that genes related to learning and memory, synaptic plasticity, and neurogenesis were upregulated in D. auratus, while genes related to apoptosis were upregulated in túngara frogs, suggesting that these cellular processes could contribute to the differences in behavioral flexibility and spatial learning we observed between poison frogs and túngara frogs.

2.
Nano Lett ; 2020 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-32250636

RESUMO

Microfabricated mechanical resonators enable precision measurement techniques from atomic force microscopy to emerging quantum applications. The resonance frequency-based physical sensing combines high precision with long-term stability. However, widely used Si3N4 resonators suffer from frequency sensitivity to temperature due to the differential thermal expansion vs the Si substrates. Here we experimentally demonstrate temperature- and residual stress-insensitive 16.51 MHz tuning fork nanobeam resonators with nonlinear clamps defining the stress and frequency by design, achieving a low fractional frequency sensitivity of (2.5 ± 0.8) × 10-6 K-1, a 72× reduction. On-chip optical readout of resonator thermomechanical fluctuations allows precision frequency measurement without any external excitation at the thermodynamically limited frequency Allan deviation of ≈7 Hz/Hz1/2 and (relative) bias stability of ≈10 Hz (≈ 0.6 × 10-6) above 1 s averaging, remarkably, on par with state-of-the-art driven devices of similar mass. Both the resonator stabilization and the passive frequency readout can benefit a wide variety of micromechanical sensors.

3.
Cell Signal ; 71: 109601, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32184196

RESUMO

As a classical signaling pathway, transforming growth factor ß (TGF-ß) has been studied in various animals for more than decade years. However, the members of TGF-ß were markedly expanded in teleost specific third and fourth rounds of whole genome duplication (WGD). Here, four smad4s named Posmad4a, Posmad4b, Posmad4c and Posmad4d were identified in Japanese flounder. Our study showed that four flounder smad4s had distinct properties in terms of their protein structure, expression pattern, protein interaction and subcellular localization. PoSMAD4a/b were mainly located in the cytoplasm, and could co-localize in the nucleus with PoSMAD3a after TGF-ß activator stimulation. PoSMAD4c was mainly located in nucleus, whereas PoSMAD4d distributed in the whole cell. Both PoSMAD4c and PoSMAD4d could co-localize in the nucleus with PoSMAD3b after TGF-ß activator stimulation. Furthermore, Posmad4c responded most strongly to TGF-ß signal stimulation. Dual-luciferase reporter assay also showed that Posmad4c could specifically up-regulate the TGF-ß signal luciferase reporter gene, Posmad4b could enhance Wnt signal luciferase reporter gene, while both Posmad4b and Posmad4d could markedly up-regulate Notch signal reporter gene. All results indicated that Posmad4a/b/c/d had significantly functional differences among TGF-ß, Notch and Wnt signaling pathways. Our study provided important understanding to the biology of smad4s and its pathway crosstalk in teleost.

5.
Curr Microbiol ; 77(3): 491-499, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31832840

RESUMO

Bacillus sp. TYF-LIM-B05, which is isolated from spoilage vinegar, is resistant to high temperature, high concentrated alcohol, acid, and salt, and can produce ethanol from mono-, di-, polysaccharide, and complex biomass as the sole carbon source. Thus, this strain is a potential candidate for consolidated bioprocessing (CBP) of fermenting lignocellulose to ethanol in a single step. To provide insight into the key enzymes involved in lignocellulose degradation and ethanol production, a draft genome of TYF-LIM-B05 was developed in this study. The results indicated that 348 genes are related to carbohydrate transport and metabolism according to the clusters of orthologous groups of proteins and annotated 187 CAZy domains from a total of 61 different families. The presence of genes encoding laccases, quinone oxidoreductases/reductases, and aryl-alcohol dehydrogenases further implies that TYF-LIM-B05 has the potential to degrade lignin. Remarkably, this strain has the ability to catalyze the oxidative decarboxylation of pyruvate to acetyl-CoA by pyruvate dehydrogenase complexes. The genomic information provided in this study will help researchers to better understand the mechanisms of the lignocellulose degradation and ethanol production pathway in thermophiles.

6.
J Exp Zool B Mol Dev Evol ; 334(1): 25-36, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31743605

RESUMO

Opsins play important roles in the image-forming visual pathways and numerous biological systems such as the biological clock and circadian rhythm. However, the nonvisual opsins involved in nonimage forming process are not clear to date. The aim of this study was to characterize nonvisual opsins in Paralichthys olivaceus. A total of 24 nonvisual opsin genes were identified. Expressions of these genes in eye, brain, heart, testis, and fin were investigated by quantitative real-time polymerase chain reaction (qRT-PCR). Testis contained a surprisingly large number of nonvisual opsins including Opn4m2, Tmt2a, Tmt3b, Opn3, RRH, Opn7a, and Opn7b. Syntenic and phylogenetic analyses confirmed that the RGRa and RGRb originated from the teleost-specific genome duplication (TSGD). qRT-PCR results demonstrated high RGRa and RGRb expression in the eye, while the expression levels in the brain, heart, testis, and fin were relatively weak. In situ hybridization results presented here revealed the presence of both RGRa and RGRb mRNA-positive signals in the ganglion cell layer but absence in the intracellular compartment of retinal pigment epithelium (RPE) and Müller glial cells. Therefore, we hypothesized that RGRa and RGRb had undergone subfunctionalization in P. olivaceus after TSGD. In conclusion, this study provides novel insights into the evolutionary fates of the RGR genes, still, further studies need to be done to explore the mechanism about the lack of RGR genes' expression in RPE.

7.
Stem Cell Res Ther ; 10(1): 386, 2019 12 16.
Artigo em Inglês | MEDLINE | ID: mdl-31843004

RESUMO

Acute respiratory distress syndrome (ARDS) is a devastating hypoxemic respiratory failure, characterized by disruption of the alveolar-capillary membrane barrier. Current management for ARDS remains supportive, including lung-protective ventilation and a conservative fluid strategy. Mesenchymal stem cells (MSCs) have emerged as a potentially attractive candidate for the management of ARDS through facilitating lung tissue regeneration and repair by releasing paracrine soluble factors. Over the last decade, a variety of strategies have emerged to optimize MSC-based therapy. Among these, the strategy using genetically modified MSCs has received increased attention recently due to its distinct advantage, in conferring incremental migratory capacity and, enhancing the anti-inflammatory, immunomodulatory, angiogenic, and antifibrotic effects of these cells in numerous preclinical ARDS models, which may in turn provide additional benefits in the management of ARDS. Here, we provide an overview of recent studies testing the efficacy of genetically modified MSCs using preclinical models of ARDS.

8.
Diabet Med ; 36(12): 1671-1678, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31392737

RESUMO

AIM: To evaluate the value and feasibility of capillary glucose assessment, combined with other non-laboratory measures, in screening for diabetes and prediabetes in the community. METHODS: In this cross-sectional study, we assessed fasting capillary glucose, fasting plasma glucose, and both capillary glucose and plasma glucose values after 2-h oral glucose tolerance tests in a total of 3736 samples. We determined the optimal threshold of capillary glucose using receiver-operating characteristic curve analysis. The effect of screening methods using capillary glucose combined with other variables, such as age, BMI and waist circumference, was assessed according to area under the receiver-operating characteristic curve. RESULTS: There was a strong positive correlation between capillary glucose and venous plasma glucose. The area under the curve for the model using fasting capillary glucose to screen for impaired fasting glucose was 0.722, while that for the model using capillary glucose after a 2-h oral glucose tolerance test to screen for impaired glucose tolerance was 0.916. The area under the curve for the model using fasting capillary glucose to screen for diabetes was 0.835, while that for the model using 2-h oral glucose tolerance test capillary glucose was 0.912. The area under the curve for the model using fasting capillary glucose + 2-h oral glucose tolerance test capillary glucose to screen for diabetes was 0.945. The discriminatory capability of models using capillary glucose was somewhat improved by adding non-laboratory variables. CONCLUSIONS: Capillary glucose could be an alternative for screening for diabetes and prediabetes, especially in low-resource areas.

9.
Fish Shellfish Immunol ; 93: 269-277, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31306762

RESUMO

As an isoform of Rho family GTPases, RhoB plays a pivotal role in cytoskeletal organization, cell proliferation, apoptosis and immune response. However, the regulatory mechanisms of RhoB expression in aquatic animals are still unknown. In the present study, we first construct Vibrio anguillarum infection model in S. maximus, including susceptible and resistant individuals. Then the temporal expression of RhoB was detected after V. anguillarum challenge using qRT-PCR and found that RhoB transcripts were significantly induced in the liver, gill and blood despite of differential expression levels and responsive time points. In addition, the mRNA levels of RhoB in resistant individuals were significantly higher than in susceptible ones. The length of 2083 bp sequences of RhoB promoter was cloned and characterized. Moreover, DNA methylation of the RhoB promoter was measured by bisulfite sequencing (BSP) and hypo-methylated was detected in the CpG islands. Three SNPs (-1590, -1575 and -1449) and two haplotypes in the promoter region of RhoB were identified to be associated with V. anguillarum resistance in turbot by association analysis in group 17-R and 17-S. Deletion analysis indicated that these SNPs could negatively mediate the activity of RhoB promoter. Site-directed mutagenesis and qRT-PCR of individuals with different genotypes demonstrated that -1575 T/A polymorphism affected promoter activity. Further study showed that this mutation altered the binding site of the transcription factor CREB. Co-transfection of SmCREB and RhoB promoter was performed in HEK293T cells which confirmed the -1575 allelic differences on transcriptional activity, with the susceptibility allele showing reduced activity. Taken together, our findings implicate that losing of binding of CREB to SmRhoB promoter due to -1575T/A polymorphisms enhances SmRhoB expression in resistant turbot, which provide insights into the effect of SmRhoB expression in response to V. anguillarum infection.


Assuntos
Doenças dos Peixes/imunologia , Linguados/genética , Polimorfismo de Nucleotídeo Único/imunologia , Vibrio/fisiologia , Proteína rhoB de Ligação ao GTP/imunologia , Animais , Proteína de Ligação a CREB/genética , Proteína de Ligação a CREB/metabolismo , Suscetibilidade a Doenças/imunologia , Suscetibilidade a Doenças/veterinária , Doenças dos Peixes/genética , Proteínas de Peixes/genética , Proteínas de Peixes/imunologia , Linguados/imunologia , Regulação da Expressão Gênica/imunologia , Haplótipos/imunologia , Mutação , Regiões Promotoras Genéticas/genética , Regiões Promotoras Genéticas/imunologia , Vibrioses/imunologia , Vibrioses/veterinária , Proteína rhoB de Ligação ao GTP/genética
10.
Sci Total Environ ; 690: 654-666, 2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31301506

RESUMO

Potassium monopersulfate (PMS) without a catalyst as cathode electron acceptor was first established to improve the electricity generation performance of a microbial fuel cell (MFC) in this study. The work investigated the performance with pure PMS (PPMS) and compound PMS (CPMS). The concentration and initial pH of PMS had an effect on the electricity generation, which increased with higher PMS concentration and lower catholyte pH. In the PPMS-MFC system, the maximum voltage (0.972 V), power density (16.37 W/m3), optimal exchange current density (2.000 A/m3) and minimum polarization impedance (Rp: 97.33 Ω) were reached at 10 mM PMS and pH 3.0. However, the maximum power density (8.60 W/m3) was exhibited at 70 mM PMS and pH 3.0 in the CPMS system. Additionally, high COD removals of 99.41% and 98.71% in anode chambers were obtained in the two systems, respectively. Sulfate radicals (SO4-) and hydroxyl radicals (OH) played significant roles in the PPMS-MFC, while HClO was also a contributor in addition to SO4- and OH in the CPMS-MFC. Furthermore, SO4- and OH was generated in situ in the cathode to promote the reduction reaction. The inorganic anion had different effects on electricity generation. Finally, while energy was recovered, rhodamine B (RhB) was added to the cathode chamber and then removed successfully in PPMS-MFC system. This work confirmed that only PMS could be activated by bio-electrochemical method, which is an energy-saving, environmentally friendly and effective activation approach, and thus, it could be used as an efficient acceptor in a MFC.


Assuntos
Fontes de Energia Bioelétrica , Compostos de Potássio/química , Sulfatos/química , Técnicas Eletroquímicas , Eletrodos , Elétrons
11.
Curr Zool ; 65(3): 317-321, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31263490

RESUMO

In túngara frogs, female mate choice requires remembering the location and/or calls of preferred males who advertise from fixed positions within a breeding pond. A previous study found that, when solving a place discrimination task in the laboratory, female túngara frogs were able to learn a visual cue to solve the task, whereas males were not. In that task, male performance appeared to be inhibited, in part, by their attempt to use egocentric cues. We tested whether the sex difference in place learning previously reported would generalize to other training parameters with different cues available by eliminating the potential to use egocentric cues and increasing the number of trials per day. As before, frogs were given a choice between a red or yellow door, one of which led to shelters and return to their home cage. In the current testing conditions, we detected a preference for the red door; thus, we only considered frogs rewarded to the yellow door. Training was associated with an increase in correct choices and an increased preference for the yellow door. However, there was no evidence for a sex difference in learning. In summary, under the current training conditions, we found that the apparent female advantage in place learning was no longer evident. Future studies that investigate sex differences in cue preference and/or ability to switch among cues will further illuminate the conditions under which sex differences in learning are manifest in túngara frogs.

12.
Enzyme Microb Technol ; 128: 22-25, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31186106

RESUMO

A catalase-inorganic hybrid nanoflowers (Cat-HNFs)-based colorimetric platform was prepared for visual detection of hydrogen peroxide (H2O2). Catalase via coordination was self-assembled and embedded in the copper phosphate crystals by sonication. Under the designed colorimetric system, Cat-HNFs showed linear absorbance responses toward H2O2 in the concentration range of 1 µM to 1 mM, and the detection limit was 1 µM. Compared to free catalase, Cat-HNFs exhibited enhanced activity and stability for H2O2 detection. Moreover, after 5 cycles via centrifugal recovery, Cat-HNFs still remained 68% of their initial activity. The detection result in human serum suggested Cat-HNFs can be a promisng candidate for the detection of H2O2 in biomedical field.


Assuntos
Técnicas Biossensoriais/métodos , Calorimetria/métodos , Catalase/metabolismo , Cobre/metabolismo , Peróxido de Hidrogênio/análise , Nanoestruturas/química , Fosfatos/metabolismo , Catalase/química , Cobre/química , Fosfatos/química , Sonicação
13.
Fish Shellfish Immunol ; 92: 450-459, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31207302

RESUMO

PIK3CA has been extensively investigated from its molecular mechanism perspective and association with its mutations in different types of cancers. However, little has been reported regarding the pathological significance of PIK3CA expression in teleost. Here, in our present study, three PIK3CA genes termed SmPIK3CAa, SmPIK3CAb and SmPIK3CA-like were firstly identified in the genome of turbot S. maximus. Although these three genes located in different chromosomes, all of them share the same five domains. Phylogenetic and synteny analysis indicated that SmPIK3CAa, SmPIK3CAb and SmPIK3CA-like were three paralogs that may originate from duplication of the same ancestral PIK3CA gene. Subcellular localization analysis confirmed the cytoplasm distribution of these three paralogs. All three SmPIK3CA were ubiquitously expressed in examined tissues in turbot, with the higher expression levels in immune-related tissues such as blood, spleen, kidney, gills and intestines. Upon Vibrio anguillarum challenge, SmPIK3CAa and SmPIK3CA-like transcripts were significantly induced in spleen, intestine and blood despite of differential expression levels and responsive time points. Additionally, individuals in resistant group showed significantly higher expression level of both two genes than in the susceptible group. Moreover, four SNPs (102, 2530, 3027 and 3060) and one haplotype (Hap2) located in exon region of SmPIK3CA-like were identified and confirmed to be associated with V. anguillarum resistance in turbot by association analysis in different populations. Taken together, these results suggested that functional differentiation occurred in three SmPIK3CA paralogs with Vibrio anguillarum resistance and SmPIK3CAa and SmPIK3CA-like probable play potential roles in innate immune response to pathogenic invasions in turbot.


Assuntos
Doenças dos Peixes/imunologia , Linguados/genética , Linguados/imunologia , Regulação da Expressão Gênica/imunologia , Imunidade Inata/genética , Fosfatidilinositol 3-Quinase/genética , Fosfatidilinositol 3-Quinase/imunologia , Sequência de Aminoácidos , Animais , Proteínas de Peixes/química , Proteínas de Peixes/genética , Proteínas de Peixes/imunologia , Perfilação da Expressão Gênica/veterinária , Fosfatidilinositol 3-Quinase/química , Filogenia , Alinhamento de Sequência/veterinária , Vibrio/fisiologia , Vibrioses/imunologia , Vibrioses/veterinária
14.
J Exp Biol ; 222(Pt 11)2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31182504

RESUMO

A fundamental question in cognitive science is whether an animal can use a cognitive map. A cognitive map is a mental representation of the external world, and knowledge of one's place in this world, that can be used to determine efficient routes to any destination. Many birds and mammals are known to employ a cognitive map, but whether other vertebrates can create a cognitive map is less clear. Amphibians are capable of using beacons, gradients and landmarks when navigating, and many are proficient at homing. Yet only one prior study directly tested for a cognitive map in amphibians, with negative results. Poison frogs exhibit unusually complex social and spatial behaviors and are capable of long-distance homing after displacement, suggesting that they may be using complex spatial navigation strategies in nature. Here, we trained the poison frog Dendrobates auratus in a modified Morris water maze that was designed to suppress thigmotaxis to the maze wall, promoting exploration of the arena. In our moat maze, the poison frogs were able to use a configuration of visual cues to find the hidden platform. Moreover, we demonstrate that they chose direct paths to the goal from multiple random initial positions, a hallmark of a cognitive map. The performance of the frogs in the maze was qualitatively similar to that of rodents, suggesting that the potential to evolve a cognitive map is an evolutionarily conserved trait of vertebrates.

15.
Hum Reprod ; 34(7): 1325-1333, 2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31216361

RESUMO

STUDY QUESTION: What is the relationship between pre-pregnancy maternal glucose levels and fecundability in Chinese couples? SUMMARY ANSWER: Elevated pre-pregnancy maternal glucose levels were associated with fecundability, as reflected by prolonged time to pregnancy (TTP) among the couples with no prior gravidity. STUDY DESIGN, SIZE, DURATION: Based on the National Free Pre-conception Check-up Projects supported by the Chinese government, 2 226 048 eligible couples attempting first pregnancy and participating in the project from 2015 to 2016 were included. They were followed-up for 1 year or until they reported pregnancy. PARTICIPANTS/MATERIALS, SETTINGS, METHODS: The Kaplan-Meier method was used to estimate the cumulative pregnancy rate in each menstrual cycle, and the discrete-time analogue of the Cox models was used to estimate the fecundability odds ratios (FORs) and 95% CIs by different pre-pregnancy maternal glucose levels (impaired fasting glucose (IFG) or diabetes as compared to normal). MAIN RESULTS AND THE ROLE OF CHANCE: The cumulative pregnancy rate for 12 cycles of the normal fasting plasma glucose (FPG) level group was 42.29%, significantly higher than that of the IFG (35.52%) and diabetes groups (31.52%). After adjusting for confounding factors, the FORs were 0.82 (95% CI: 0.81-0.83) and 0.74 (95% CI: 0.72-0.76) for the IFG and diabetes groups, respectively, as compared to the normal group. The association between pre-pregnancy maternal FPG levels and the FORs was non-linear, and the optimal FPG level for greatest fecundability (shortest TTP) was 3.90-4.89 mmol/L. LIMITATIONS, REASONS FOR CAUTION: The findings from this register-based cohort study require cautious interpretation given that information bias would be inevitable for single FPG measurements and for TTP calculations that were based on telephone follow-up information. Additionally, because couples who achieved pregnancy during their first menstrual cycle in the study were excluded, the pregnancy rates reported were low and possibly biased. WIDER IMPLICATIONS OF THE FINDINGS: The current report suggests that elevated pre-pregnancy maternal glucose levels were associated with prolonged TTP. Early evaluation and preventive treatment for female partners with IFG or diabetes in a pre-pregnancy examination are necessary. STUDY FUNDING/COMPETING INTEREST(S): Funding was provided by the National Key Research and Development Program of China (grants No. 2016YFC1000300 and 2016YFC1000307), the National Natural Science Foundation of China (grant No. 81872634), the CAMS Innovation Fund for Medical Sciences (grant No. 2018-I2M-1-004), the National Human Genetic Resources Sharing Service Platform (grant No. 2005DKA21300) and the National Population and Reproductive Health Science Data Center (grant No. 2005DKA32408), People's Republic of China. The authors have no conflicts of interest to declare. TRIAL REGISTRATION NUMBER: N/A.

16.
Dalton Trans ; 48(20): 7015-7024, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-31049534

RESUMO

A new type of Pd@mSiO2 composite nanospheres with controlled pore structure, consisting of internal Pd cores and controlled mesoporous silica shells, has been prepared by a facile one-pot method. The thickness and pore size of the shell could be easily tuned by changing the amounts of TEOS and the hydrophobic block length, respectively, during synthesis. In this perspective, the effects of CTAB concentration, pH, and TEOS concentration on the monodisperse sphere morphology of Pd@mSiO2 nanoparticles (NPs) were investigated. In addition, a nucleation mechanism was proposed. Hydrogenation of nitrobenzene to aniline was used as a model reaction to discuss the effect of pore size on the transport rate of the reactants and the product selectivity in metal-catalyzed hydrogenation. The catalysts exhibited a high conversion rate and significantly enhanced stability, leading to a higher recyclability without loss of catalytic activity compared to conventional supported catalysts and commercial catalysts.

17.
Gene ; 708: 21-29, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31082502

RESUMO

GATA5 is a member of the GATA transcription factor family, which serves essential roles in varieties of cellular functions and biological processes. In this study, we have accomplished the molecular cloning, bioinformatic analysis and preliminary function study of C. semilaevis GATA5. The full-length cDNA nucleotide sequence is 1955 bp, with a coding sequence of 1167 bp, which encodes a polypeptide of 388 amino acids. Homology, phylogenetic, gene structure and synteny analysis showed that C. semilaevis GATA5 was highly conserved among vertebrates. Tissue distribution pattern exhibited that C. semilaevis GATA5 was significantly expressed in heart, intestine, liver, kidney and gonad, with a sexual dimorphic feature observed in testis and ovary. Embryonic development expression profiles showed that C. semilaevis GATA5 transcripts increased at the blastula stage, and peaked at the heat-beating period. Strong signals were detected at spermatids of male testis and stage III oocytes of female ovary by ISH. The expression of C. semilaevis GATA5 was regulated by 17α-MT and E2 after hormone stimulation to the ovary. Together, all the results pointed out that GATA5 might play a vital role during gonadal maturation and the reproductive cycle of C. semilaevis. This study lays the foundation for further researches on the sex control breeding in tongue sole.


Assuntos
Proteínas de Peixes/fisiologia , Linguados/fisiologia , Fator de Transcrição GATA5/fisiologia , Regulação da Expressão Gênica no Desenvolvimento , Reprodução/fisiologia , Animais , Cruzamento/métodos , Embrião não Mamífero , Feminino , Pesqueiros , Perfilação da Expressão Gênica , Masculino , Ovário/crescimento & desenvolvimento , Ovário/metabolismo , Filogenia , Sintenia , Testículo/crescimento & desenvolvimento , Testículo/metabolismo
18.
Fish Shellfish Immunol ; 89: 477-485, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30940579

RESUMO

To study the effect of Edwardsiella tarda infection on miRNAs expression profile in Japanese flounder, fish were injected intraperitoneally with E. tarda. The miRNAs involved in regulating immune responses were analyzed by high-throughput sequencing. A total of 164 mature miRNAs were identified, of which 17 miRNAs were differentially expressed (DE miRNAs) after E. tarda infection, indicating that they were immune-related miRNAs. To further examine the relationship between the miRNAs and their predicted target mRNAs, a total of 22 predicted target mRNAs, mainly related to endocytic signaling pathway, NF-κB signaling pathway, and p53 signaling pathway, were detected with miRNA mimics in HEK-293T cells by dual-luciferase reporter experiments. Finally, we confirmed that insulin receptor substrate-2 (IRS2a and IRS2b) were regulated by miR-7a. And the target sites of the 3' untranslated region (UTR) of IRS2a and IRS2b were verified by dual-luciferase reporter experiments. Furthermore, we found that the E. tarda and LPS significantly increased host miR-7a expression. In vivo and in vitro studies revealed that IRS2-mediated PI3K/AKT/GSK3ß signaling pathway was suppressed. Taken together, these results implied that miR-7a might be a key regulator of PI3K/AKT/GSK3ß signaling pathway via suppressing the IRS2a and IRS2b genes.


Assuntos
Edwardsiella tarda/fisiologia , Proteínas de Peixes/genética , Linguados/imunologia , Proteínas Substratos do Receptor de Insulina/genética , MicroRNAs/metabolismo , Transdução de Sinais/genética , Animais , Proteínas de Peixes/metabolismo , Linguados/genética , Linguados/metabolismo , Proteínas Substratos do Receptor de Insulina/metabolismo
19.
J Immunol Res ; 2019: 3737890, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30915370

RESUMO

Gram-negative bacterial sepsis accounts for up to 50% worldwide sepsis that causes hospital mortality. Acute kidney injury (AKI), a common complication of Gram-negative bacterial sepsis, is caused by Toll-like receptor 4 (TLR4) activation. Lipopolysaccharide (LPS) is an endotoxin in Gram-negative bacteria and is recognized specifically by TLR4, which initiates innate immune response. Also, TLR4 signaling pathway activation is essential in response to LPS infection. CD38 is one of the well-known regulators of innate immunity, whose dysregulation contributes to sepsis. Many studies have proven that an attenuated Gram-positive bacterium induces sepsis in a CD38-blocking model. However, the pathogenesis of Gram-negative bacteria-induced sepsis in a CD38-/- mouse model remains unclear. The aim of this study is to investigate whether kidney injury is still attenuated in a LPS-induced CD38-/- sepsis model and identify the potential mechanism. We assess the severity of kidney injury related to proinflammatory cytokine expressions (IFN-γ, TNF-α, IL-1ß, and IL-6) in WT and CD38-/- mice. Our results showed more aggravated kidney damage in CD38-/- mice than in WT mice, accompanied with an increase of proinflammatory cytokine expression. In addition, compared with CD38-/-TLR4mut mice, we found an increase of TLR4 expression and mRNA expression of these cytokines in the kidney of CD38-/- mice, although only increased IFN-γ level was detected in the serum. Taken together, these results demonstrated that an increased TLR4 expression in CD38-/- mice could contribute to the aggravation of AKI through boosting of the production of IFN-γ.


Assuntos
ADP-Ribosil Ciclase 1/metabolismo , Lesão Renal Aguda/imunologia , Bactérias Gram-Negativas/fisiologia , Sepse/imunologia , Receptor 4 Toll-Like/metabolismo , ADP-Ribosil Ciclase 1/genética , Animais , Citocinas/metabolismo , Regulação da Expressão Gênica , Imunidade Inata , Mediadores da Inflamação/metabolismo , Interferon gama/metabolismo , Lipopolissacarídeos/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Receptor 4 Toll-Like/genética
20.
Epidemiol Infect ; 147: e83, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30869019

RESUMO

The aim of this meta-analysis was to provide a comprehensive overview of human immunodeficiency virus (HIV)-1 subtypes and to investigate temporal and geographical trends of the HIV-1 epidemic among men who have sex with men (MSM) in China. Chinese and English articles published between January 2007 and December 2017 were systematically searched. Pooled HIV-1 prevalence was calculated, and its stability was analysed using sensitivity analysis. Subgroups were based on study time period, sampling area and prevalence. Publication bias was measured using Funnel plot and Egger's test. A total of 68 independent studies that included HIV-1 molecular investigations were eligible for meta-analysis. Circulating recombinant form (CRF) 01_AE (57.36%, 95% confidence interval (CI) 53.76-60.92) was confirmed as the most prevalent HIV-1 subtype among MSM in China. Subgroup analysis for time period found that CRF01_AE steadily increased prior to 2012 but decreased during 2012-2016. Further whereas CRF07_BC increased over time, B/B' decreased over time. CRF55_01B has increased in recent years, with higher pooled estimated rate in Guangdong (12.22%, 95% CI 10.34-13.17) and Fujian (8.65%, 95% CI 4.98-13.17) provinces. The distribution of HIV-1 subtypes among MSM in China has changed across different regions and periods. HIV-1 strains in MSM are becoming more complex. Long-term molecular monitoring in this population remains necessary for HIV-1 epidemic control and prevention.

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