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2.
Tissue Eng Regen Med ; 15(3): 341-350, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30603559

RESUMO

miR-214 plays a major role in the self-renewal of skin tissue. However, whether miR-214 regulates the proliferation and differentiation of human hair follicle stem cells (HFSCs) is unknown. Primary HFSCs were isolated from human scalp skin tissue, cultured, and identified using flow cytometry. An miR-214 mimic and inhibitor were constructed for transfection into HFSCs. The MTS and colony formation assays examined cell proliferation. Immunofluorescence detected the localization and expression levels of TCF4, ß-catenin, and differentiation markers. Luciferase reporter and TOP/FOP Flash assays investigated whether miR-214 targeted EZH2 and regulated the Wnt/ß-catenin signaling pathway. Western blot determined the expression levels of enhancer of zeste homolog 2 (EZH2), Wnt/ß-catenin signaling-related proteins, and HFSC differentiation markers in cells subjected to miR-214 transfection. miR-214 expression was remarkably decreased during the proliferation and differentiation of HFSCs into transit-amplifying (TA) cells. Downregulation of miR-214 promotes the proliferation and differentiation of HFSCs. Overexpression of miR-214 led to decreased expression of EZH2, ß-catenin, and TCF-4, whereas downregulation of miR-214 resulted in increased expression of EZH2, ß-catenin, and TCF-4 as well as TA differentiation markers. Immunofluorescence assay revealed that inhibiting miR-214 triggered the entry of ß-catenin and TCF-4 into the nucleus. The luciferase reporter and TOP/FOP Flash assays demonstrated that miR-214 directly targets EZH2 and affects Wnt/ß-catenin signaling. The miR-214/EZH2/ß-catenin axis could be considered a candidate target in tissue engineering and regenerative medicine for HFSCs.

3.
Artigo em Inglês | MEDLINE | ID: mdl-27595294

RESUMO

In order to address the issue of excessive intake of aluminium (Al) from Al-containing food additives in the Chinese diet, this study conducted a dietary exposure assessment of Al in the general population based on the national surveillance data of Al content in foods and national food consumption data. It was found that the mean dietary exposure of the whole Chinese population to Al from Al-containing food additives was 1.795 mg kg‒1 bw week‒1, not exceeding the PTWI, while high dietary exposures (e.g., 97.5th percentile) to Al were 7.660 and 2.103-2.903 mg kg‒1 bw week‒1 for children, respectively, both exceeding the PTWI. It was found that the dietary exposure to Al for 32.5% of the total Chinese population and 42.6% of children aged 4-6 years exceeded the PTWI. Wheat flour and wheat-based products are the main source of dietary A l exposure (85% of the total intake); and puffed foods are the major source of Al intake for children. These findings suggested that consumption of Al-containing food additives could be a health concern for consumers with high food consumption (97.5th percentile) and children under the age of 14 years.


Assuntos
Alumínio/administração & dosagem , Alumínio/análise , Dieta , Exposição Ambiental/análise , Aditivos Alimentares/química , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Alumínio/efeitos adversos , Criança , Pré-Escolar , China , Exposição Ambiental/efeitos adversos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medição de Risco , Triticum , Adulto Jovem
4.
Biomed Environ Sci ; 29(5): 365-73, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27353711

RESUMO

OBJECTIVE: Benzyl butyl phthalate (BBP) is a plasticizer used in food contact materials. Dietary exposure to BBP might lead to reproduction and developmental damages to human. The present paper was aimed to assess the health risk of BBP dietary exposure in Chinese population. METHODS: The BBP contents were detected in 7409 food samples from 25 food categories by gas chromatography-mass spectrometry operated in selected ion monitoring (SIM) mode. The dietary exposures of BBP in different age and sex groups were estimated by combining the content data with food consumption data derived from 2002 China National Nutrient and Health Survey, and evaluated according to the tolerable daily intake (TDI) of BBP established by European Food safety Agency. RESULTS: It was found that BBP was undetectable in most samples and the highest level was 1.69 mg/kg detected in a vegetable oil sample. The average dietary exposure of BBP in people aged ⋝2 years was 1.03 µg/kg bw per day and the highest average exposure was found in 2-6 years old children (1.98 µg/kg bw per day). The BBP exposure in 7-12 months old children excessed 10% of tolerable daily intake (TDI) in worst scenario. . CONCLUSION: The health risk of BBP dietary exposure in Chinese population is low and, considering BBP alone, there is no safety concern.


Assuntos
Exposição Ambiental , Poluentes Ambientais/análise , Contaminação de Alimentos/análise , Embalagem de Alimentos , Ácidos Ftálicos/análise , Plastificantes/análise , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , China , Dieta , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
5.
Adv Mater ; 28(28): 5878-85, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27159546

RESUMO

Flexible metal oxide/graphene oxide hybrid multi-gate neuromorphic transistors are fabricated on flexible conducting graphene substrates. Dendritic integrations in both spatial and temporal modes are emulated, and spatiotemporal correlated logics are obtained. A proof-of-principle visual system model for emulating Lobula Giant Motion Detector neuron is also investigated. The results are of great significance for flexible sensors and neuromorphic cognitive systems.

6.
Adv Mater ; 28(18): 3557-63, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-26972820

RESUMO

Proton-conducting graphene oxide electrolyte films with very high electric-double-layer capacitance are used as the gate dielectrics for oxide-based neuron transistor fabrication. Paired-pulse facilitation, dendritic integration, and orientation tuning are successfully emulated. Additionally, neuronal gain controls (arithmetic) are also experimentally demonstrated. The results provide a new-concept approach for building brain-inspired cognitive systems.


Assuntos
Neurônios , Encéfalo , Cognição , Grafite , Óxidos , Prótons , Transistores Eletrônicos
7.
Biomed Environ Sci ; 28(7): 477-85, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26248731

RESUMO

OBJECTIVE: To understand the dietary intake levels of trans fatty acids (TFA) in a Chinese population and establish a basis for health risk assessment of trans fatty acids. METHODS: The TFA contents data of 2613 food items and food consumption data of 10,533 people aged 3 years and above in two large cities in China were matched and a simple assessment method was used to estimate the distribution of dietary TFA intake. RESULTS: The mean content of TFA was highest in margarine (1.68 ± 0.83 g/100g), followed by chocolate and candy (0.89 ± 2.68 g/100g), edible vegetable oils (0.86 ± 0.82 g/100g), milk (0.83 ± 1.56 g/100g), and bakery foods (0.41 ± 0.91 g/100g). TFA intake accounted for 0.34%, 0.30%, 0.32%, and 0.29% of the total energy intake in the 3-6, 7-12, 13-17, and ⋝18 year age groups, respectively. Of the populations studied, 0.42% demonstrated TFA intakes (as percentage of energy intake) greater than 1%. The main sources of dietary TFA intake were edible vegetable oils, milk, mutton, and beef, and baked foods, which accounted for 49.8%, 16.56%, 12.21%, and 8.87%, respectively. CONCLUSION: The current intake of TFA among people in two cities did not appear to be of major health concern regarding the threshold of TFA intake as the percentage of total energy recommended by the World Health Organization. Because most TFA were derived from industrially processed foods, the government should reinforce nutrition labeling and regulate food producers to further reduce TFA in food and to provide scientific instruction for consumers to make sound choices.


Assuntos
Gorduras na Dieta/análise , Ingestão de Energia , Análise de Alimentos , Alimentos , Ácidos Graxos trans/análise , Adolescente , Análise de Variância , Criança , Pré-Escolar , China , Inquéritos sobre Dietas , Gorduras na Dieta/administração & dosagem , Gorduras na Dieta/metabolismo , Feminino , Alimentos/normas , Humanos , Masculino , Inquéritos e Questionários , Ácidos Graxos trans/administração & dosagem , Ácidos Graxos trans/metabolismo
8.
Biomed Environ Sci ; 27(12): 950-9, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25484011

RESUMO

OBJECTIVE: To evaluate the endocrine disrupting effects of cadmium (Cd) using OECD enhanced TG407 test guideline. METHODS: Sprague-Dawley (SD) rats were randomly divided into six groups and accordingly administered with 0, 1, 2.5, 5, 10, 20 mg/kg•BW/day of Cd by gavage for 28 days. Body weight, food consumption, hematology, biochemistry, sex hormone levels, urinary ß2-microglobulin, organ weights and histopathology and estrous cycle were detected. RESULTS: Cd could significantly decrease animals' body weight (P<0.05). Serum luteinizing hormone (LH) at 10-20 mg/kg•BW groups and testosterone (T) at 2.5 and 10 mg/kg•BW groups decreased significantly (P<0.05). However, no statistically significant change was found in urinary ß2-microglobulin among Cd-treatment groups (P>0.05). Endpoints related to female reproduction including uterus weight and histopathological change at 10-20 mg/kg•BW groups showed significant increase (P<0.05). While among male rats in 2.5, 10, 20 mg/kg•BW groups, weight of prostate, thyroids, and seminal vesicle glands significantly decreased (P<0.05). Moreover, no histopathological change was observed in kidney. CONCLUSION: Results suggested that Cd can cause endocrine disrupting effects in SD rats. Comparing with possible renal toxicity of Cd, its toxicity on endocrine system was more sensitive.


Assuntos
Cádmio/toxicidade , Disruptores Endócrinos/toxicidade , Hormônios/sangue , Rim/efeitos dos fármacos , Animais , Peso Corporal/efeitos dos fármacos , Ingestão de Alimentos/efeitos dos fármacos , Feminino , Masculino , Organização para a Cooperação e Desenvolvimento Econômico , Distribuição Aleatória , Ratos Sprague-Dawley , Útero/efeitos dos fármacos , Microglobulina beta-2/urina
9.
Biomed Environ Sci ; 27(11): 912-6, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25374026

RESUMO

The method has been developed to accurately identify the magnitude of health risks and provide scientific evidence for implementation of risk management in food safety. It combines two parameters including consequence and likelihood of adverse effects based on risk matrix. Score definitions and classification for the consequence and the likelihood of adverse effects are proposed. The risk score identifies the intersection of consequence and likelihood in risk matrix represents its health risk level with different colors: 'low', 'medium', 'high'. Its use in an actual case is shown.


Assuntos
Qualidade de Produtos para o Consumidor , Análise de Alimentos/métodos , Alimentos/classificação , Ácidos Graxos trans/efeitos adversos , Ácidos Graxos trans/análise , Análise de Alimentos/estatística & dados numéricos , Humanos , Funções Verossimilhança , Medição de Risco
10.
Int J Hyg Environ Health ; 217(6): 695-701, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24680371

RESUMO

Di(2-ethylhexyl) phthalate (DEHP) is a common plasticizer used in food contact materials that has been reported as an endocrine disruptor. In the present study, DEHP concentrations were analyzed in foods in order to determine dietary exposure among the Chinese population, including the general population (aged 2-100) and four age group, that is children aged 2-6, adolescent aged 7-12, young people aged 13-17 and adults aged 18 years old and above. 1704 food samples were collected during 2011-2012 and categorized into 12 food groups which covered major foods in China. Food consumption data were taken from China National Nutrient and Health Survey which was performed in 2002 and includes data from 68,959 subjects. Mean concentrations of DEHP were combined with individual food consumption data to estimate dietary exposure. It was found that DEHP levels in foods ranged from not detected to 3.41 mg/kg, with highest mean values in meat (0.23 mg/kg) and vegetable oils (0.21 mg/kg). Mean dietary intakes of DEHP in the general population, children, and adults were 2.34, 4.51, 2.03 µg/kg bw per day, respectively. The 97.5% intakes in these populations were 5.22, 8.43, 3.64 µg/kg bw per day, respectively. The main food sources of DEHP dietary intake were cereals (39.44%), drinking water (16.94%) and meat (15.81%) in children, and cereals (44.57%), meat (15.70%) and drinking water (12.28%) for adults. These findings suggested that dietary exposure to DEHP among Chinese population was lower than tolerable daily intake of DEHP and there are no health concerns based on generally accepted exposure limits.


Assuntos
Dieta , Dietilexilftalato/análise , Exposição Ambiental/análise , Poluentes Ambientais/análise , Contaminação de Alimentos/análise , Plastificantes/análise , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , China , Humanos , Pessoa de Meia-Idade , Adulto Jovem
11.
Zhonghua Yu Fang Yi Xue Za Zhi ; 47(6): 565-8, 2013 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-24113110

RESUMO

OBJECTIVE: To develop and verify a model diet for consumers with high exposure to food chemicals. METHODS: Based on the assumption that a person might consume average amounts of several different foods but only two or three at high levels, a model diet were put forward to calculate the high exposures to certain food chemical.Important parameters of this model were selected by comparing the outputs of this model with those of a reference model-semi-probabilistic model that is based on individual data. The concentration data of lead, cadmium and mercury (45 832, 43 862 and 25 243 samples respectively) from the national risk surveillance for chemical contaminations and harmful factors (2011), and the consumption data from the national survey on nutrition and health status in Chinese population (2002) were used in this model optimization process. The final model was verified using concentration data of eight chemical contaminations from national surveillance database of 2010. RESULTS: When 90, 95 and 97.5 percentile exposure being calculated under the conditions that 7, 12, 20 and 30 food categories was used respectively, the model containing two high consumption foods (2+x model) got results lower than the reference values (the relative deviation < 0) , the model containing three high consumption foods (3+x model) got results higher than the reference values (the relative deviation > 0 ),but the relative deviation of the two models were both within 20%. The verification results showed that the dietary exposure results of 8 food contaminations got from 3+x model were all higher than and(or) close to those got from semi-probabilistic model, and the relative deviations were between -5% and 25%. CONCLUSION: The 3+x model diet can fulfill the demands of "conservative" and "accurate" on exposure assessment model, and can give reliable estimations of high exposure to food chemicals occurred in various food categories.


Assuntos
Exposição Ambiental , Contaminação de Alimentos , Humanos , Modelos Estatísticos , Modelos Teóricos
12.
Zhonghua Yu Fang Yi Xue Za Zhi ; 47(6): 569-72, 2013 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-24113111

RESUMO

OBJECTIVE: To establish the threshold of toxicological concern(TTC) approach and to apply it in the risk assessment of metabolites, degradation and reaction products of pirimicarb. METHODS: TTC decision tree approach based on Cramer classification was established and Lazar software was used to predict the genotoxicity of the seven transformation products of pirimicarb, namely, R34836, R34885, R35140, R31805, R34865, R16210 and R16192. Dietary exposure in general population as well as in six age population groups was estimated by using data from the Chinese National Nutrition and Health Survey and pirimicarb residue data from national chemical surveillance data in 2011. TTC decision tree approach was used for risk assessment and the exposure was compared with the corresponding TTC values. RESULTS: Of the seven transformations of pirimicarb active substance, namely, R34836, R34885, R35140, R31805, R34865, R16210 and R16192, the maximum dietary exposure of mean and large portion(P 97.5) were all belong to 2-6 age group. The mean exposures of the seven transformation products for 2-6 age group,were 0.0290, 0.0207, 0.0015, 0.0320, 0.0005, 0.6918 and 0.1274 µg/kg,respectively, and the corresponding P 97.5 exposures were 0.0817,0.0581,0.0042,0.0900,0.0014, 1.9459 and 0.3585 µg/kg. Besides, the mean and P 97.5 exposure of R16210 for 2-6 age group was the largest,which were 0.6918 and 1.9459 µg/kg, accounting for 46.12% and 129.73% of the TTC threshold,respectively. CONCLUSION: TTC decision tree approach is a useful tool for prior screening and primary risk assessment of the transformation products of pesticide active substance.


Assuntos
Carbamatos/toxicidade , Contaminação de Alimentos , Resíduos de Praguicidas/toxicidade , Praguicidas/toxicidade , Pirimidinas/toxicidade , Humanos , Concentração Máxima Permitida , Medição de Risco
13.
J Zhejiang Univ Sci B ; 13(8): 638-44, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22843184

RESUMO

OBJECTIVE: To investigate the relationship between renal function and clinical outcomes among patients with acute ST-segment elevation myocardial infarction (ASTEMI), who were treated with emergency percutaneous coronary intervention (PCI). METHODS: 420 patients hospitalized in Peking University First Hospital, diagnosed with ASTEMI treated with emergency (PCI) from January 2001 to June 2011 were enrolled in this study. Estimated glomerular filtration rate (eGFR) was used as a measure of renal function. We compared the clinical parameters and outcomes between ASTEMI patients combined renal insufficiency and the patients with normal renal function. RESULTS: There was a significant increase in the concentrations of fibrinogen and D-Dimer (P<0.05) and a much higher morbidity of diabetes mellitus in the group of patients with chronic kidney disease (CKD; eGFR<60 ml/(min·1.73 m(2))) (P<0.01). CKD (eGFR<60 ml/(min·1.73 m(2))) was an independent predictor of in-hospital mortality for patients hospitalized with ASTEMI receiving PCI therapy rapidly (P=0.032, odds ratio (OR) 4.159, 95% confidence interval (CI) 1.127-15.346). CONCLUSIONS: Renal insufficiency is an independent predictor of in-hospital mortality for patients hospitalized with ASTEMI treated with primary PCI.


Assuntos
Infarto do Miocárdio/diagnóstico , Insuficiência Renal/diagnóstico , Idoso , Dimerização , Eletrocardiografia/métodos , Feminino , Produtos de Degradação da Fibrina e do Fibrinogênio/química , Fibrinogênio/química , Taxa de Filtração Glomerular , Hospitalização , Humanos , Rim/fisiopatologia , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Infarto do Miocárdio/complicações , Infarto do Miocárdio/mortalidade , Intervenção Coronária Percutânea/métodos , Insuficiência Renal/complicações , Insuficiência Renal/mortalidade , Estudos Retrospectivos , Resultado do Tratamento
15.
Zhongguo Zhen Jiu ; 32(4): 293-6, 2012 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-22734372

RESUMO

OBJECTIVE: To compare the difference between muscle-tension-balance acupuncture and conventional acupuncture in the impacts on motor function and living ability of patients with drop foot and strephenopodia after stroke. METHODS: Seventy cases were randomly divided into a muscle-tension-balance acupuncture group (group A) and a conventional acupuncture group (group B), 35 cases in each one. In group A, firstly the acupuncture of weakening technique was applied to Sanyinjiao (SP 6) and Taixi (KI 3) on the musculus extensor side; secondly, the strengthening technique was adopted at Jiexi (ST 41), Shenmai (BL 62) and Yanglingquan (GB 34) on musculus flexor side. In group B, the conventional needling technique was applied to Zusanli (ST 36), Sanyinjiao (SP 6), Xuanzhong (GB 39), etc. The treatment was given once every day in either group, 10 treatments made one session and 3 sessions of treatment were required. The score of Fugl-Meyer motor function of the lower limb on the affected side and Barthel index score were assessed before and after treatment in two groups. RESULTS: After treated for 3 sessions, Fugle-Meyer motor function score and Barthel index score of the patients all increased in two groups (all P < 0.01). The results in group A were better than those in group B (both P < 0.05). The improvements were apparent in the 2nd session of treatment in group A (both P < 0.01), which were superior to those in group B (both P < 0.05). CONCLUSION: Either acupuncture therapy can improve the motor function and living ability of patients with drop foot and strephenopodia after stroke. The muscle-tension-balance acupuncture achieves the efficacy quickly and its efficacy is much better.


Assuntos
Atividades Cotidianas , Terapia por Acupuntura , Transtornos Neurológicos da Marcha/terapia , Atividade Motora , Acidente Vascular Cerebral/complicações , Pontos de Acupuntura , Adulto , Idoso , Feminino , Transtornos Neurológicos da Marcha/etiologia , Transtornos Neurológicos da Marcha/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade
16.
J Zhejiang Univ Sci B ; 13(3): 231-8, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22374616

RESUMO

BACKGROUND AND OBJECTIVE: In clinical practice, the standard of secondary prevention for coronary heart disease (CHD) is quite disappointing in China. The physicians' shortage of knowledge of secondary prevention guidelines is thought to be a key factor contributing to the inadequate and delayed translation of guidelines into clinical practice. The purpose of this study is to investigate the influence of physicians' characteristics, including their education and work experience, on their knowledge of secondary prevention in China. METHODS: A representative questionnaire survey was made of physicians from cardiology departments in 35 tertiary hospitals in China. The survey contained 19 questions on knowledge of guideline recommendations for the secondary prevention of CHD. We collected basic information about the physicians, including their educational degree, clinical practice duration/work experience and geographic region. RESULTS: In total, 864 physicians participated in the survey. Eight hundred and thirty-seven completed questionnaires were analyzed. For 6 of the 19 questions, physicians with a postgraduate degree were more likely to answer correctly than those without such a degree. For 11 of the 19 questions, physicians with more than three years' clinical experience were more likely to answer correctly than those who had less than three years' experience. For 5 of the 19 questions, physicians from eastern areas were more likely to answer correctly than those from mid/western areas. The mean total score of correct answers to the questionnaire was 11.69 points. Educational degree and clinical practice duration affected total scores significantly while practice location did not (ß=0.500, P=0.004; ß=0.979, P=0.000; and ß=0.228, P=0.162, respectively). Even if a relatively low score of 12 is taken as a threshold level of acceptable knowledge (defined as a pass), the pass rate of all physicians was only 53.9%. Educational degree and clinical practice duration affected pass rate significantly while practice location did not (95% CI: 1.222-2.248, P=0.001; 95% CI: 1.773-3.140, P=0.000; and 95% CI: 0.993-1.758, P=0.056, respectively). CONCLUSIONS: Physicians with a clinical practice duration of more than three years knew more about secondary prevention guidelines than those with less experience. Physicians with a postgraduate degree knew more about secondary prevention guidelines than those without a postgraduate degree. However, overall knowledge of secondary prevention guidelines for CHD was poor among this group of physicians from tertiary hospitals.


Assuntos
Doença das Coronárias/prevenção & controle , Prevenção Secundária/métodos , China , Competência Clínica , Estudos Transversais , Educação Médica , Humanos , Médicos , Guias de Prática Clínica como Assunto , Prevenção Secundária/normas , Inquéritos e Questionários
17.
Toxicol Mech Methods ; 22(2): 105-10, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22149966

RESUMO

RBL-2H3 mediator release assay, developed for specific IgE screening studies, was not as sensitive as passive cutaneous anaphylaxis (PCA) assay in the polyclonal antibody detection. In the present investigation, the detection sensitivity of RBL-2H3 assay was elevated by modifying the experiment protocols from choosing the proper releasing medium and optimizing the sensitization manner. The polyclonal antibody was generated from Brown Norway (BN) rats exposed to Ovalbumin (OVA). In contrast to Tyrode buffer A, RBL-2H3 cells cultured in DMEM had a lower spontaneous secretion and a higher response to antigen stimulation, both of which could help to increase the detection sensitivity. The rat sera used in the sensitization process should be diluted appropriately to avoid the proliferation-promoting effect on RBL-2H3 cells. The results of the kinetics of sensitization showed that prolonging the sensitization time and then reculturing the cells in IgE free medium for a further 24 h after the removal of rat sera could reach a marked increase in the degree of sensitization. The highest anti-OVA antibody titer detected by the modified RBL-2H3 assay was 4096, while PCA assay was 1024. These data provide evidence that the modified RBL-2H3 mediator release assay has a promising prospect in the determination of the biologic activity of polyclonal antibody.


Assuntos
Imunoglobulina E/análise , Leucemia Basofílica Aguda/metabolismo , Animais , Linhagem Celular Tumoral , Proliferação de Células , Ovalbumina/imunologia , Anafilaxia Cutânea Passiva , Ratos , Ratos Endogâmicos BN
18.
Biomed Environ Sci ; 24(6): 617-23, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22365397

RESUMO

OBJECTIVE: To evaluate dietary iodine intake and its potential risks among the Chinese population. METHODS: Individual dietary iodine intake was calculated using food consumption data multiplying by iodine concentration in foods, table salt and drinking water, followed by summing, and then compared with the corresponding age-specific reference values, including Upper Intake Level (UL) and Recommended Nutrient Intake (RNI). RESULTS: In areas with water iodine concentration (WI) lower than 150 µg/L, 80.8% of residents had iodine intake between the RNI and UL, 5.8% higher than UL, and the remaining (13.4%) lower than RNI if iodized salt was consumed. However, in the uniodized salt consumption scenario, only 1.0% of residents between RNI and UL, 1.4% higher than UL, and a large part of residents (97.6%) lower than RNI. In areas with WI higher than 150 µg/L, all residents had iodine intake between RNI and UL if iodized salt was consumed, except 10.5% and 24.9% of residents higher than UL in areas with WI at 150-300 µg/L and higher than 300 µg/L respectively. However, in the uniodized salt consumption scenario, only 1.5% and 1.7% of residents had higher iodine intake than UL respectively. CONCLUSION: The findings suggested that in general, the dietary iodine intake by the Chinese population was appropriate and safe at the present stage. People in areas with WI lower than 150 µg/L were more likely to have iodine deficiency. While people in areas with WI higher than 150 µg/L were more likely to have excessive iodine intake if iodized salt was consumed.


Assuntos
Água Potável , Iodo , Estado Nutricional , Cloreto de Sódio na Dieta , Adolescente , Criança , Pré-Escolar , China/epidemiologia , Dieta , Água Potável/química , Água Potável/normas , Feminino , Bócio/epidemiologia , Bócio/prevenção & controle , Humanos , Iodo/administração & dosagem , Iodo/análise , Iodo/deficiência , Masculino , Cloreto de Sódio na Dieta/administração & dosagem , Cloreto de Sódio na Dieta/análise
19.
Biomed Environ Sci ; 23(5): 357-62, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21112483

RESUMO

OBJECTIVES: This paper aims to investigate the uterotrophic activities of lactational exposure to combination of soy isoflavones (SIF) and bisphenol A (BPA) and to examine estrogen receptor α (ERα) and estrogen receptor ß (ERß) expressions in hypothalamus-pituitary-ovary axis and uterus. METHODS: Maternal rats that were breeding about 8 litters were randomly divided into four groups with seven dams in each group. Dams in different treatment groups received corn oil (control), 150 mg/kg BW of SIF, 150 mg/kg BW of BPA or combination of 150 mg/kg BW of SIF and 150 mg/kg BW of BPA, respectively, from postnatal day 5 to 11 (PND5-11) by gavage. On PND12 and PND70, 10 female litters were killed and hypothalamus, pituitary, ovary and uterus were collected. ERα and ERß expressions in these organs were detected with Western blotting assay. And vaginal opening time and estrus cycle were examined in animals fed for PND70. RESULTS: On PND12, the relative uterine weight of rats treated with ISF or BPA or their combination was significantly higher than that of untreated rats (P<0.05). But the relative uterine weight of rats in the co-exposure group was slightly lower than that in the group only exposed to SIF or BPA. On PND 70, however, the relative uterine weight in each treatment group was not statistically different from that in the control group (P>0.05). Vaginal opening time and estrus cycle in groups treated with SIF or BPA or their combination were similar to those in the control group (P>0.05). Exposure to SIF or BPA or their combination could up-regulate or down-regulate ERα and ERß expressions in hypothalamus, pituitary, ovary and uterus on PND12 and PND70. These regulation patterns for ERα and ERß were different in different organs at different time points. CONCLUSION: Lactational exposure to ISF or BPA or their combination could induce uterotrophic responses in neonate rats, which disappeared in later life. But these data fail to suggest a possibility for synergic actions between SIF and BPA. It was also demonstrated that the uterotrophic effects of SIF and BPA exposure might, at least, involve modification of ERα or ERß expressions in the hypothalamus-pituitary-ovary axis.


Assuntos
Receptor alfa de Estrogênio/biossíntese , Receptor beta de Estrogênio/biossíntese , Estrogênios não Esteroides , Sistema Hipotálamo-Hipofisário/efeitos dos fármacos , Lactação , Ovário/efeitos dos fármacos , Soja/química , Animais , Animais Recém-Nascidos , Compostos Benzidrílicos , Western Blotting , Regulação para Baixo , Sinergismo Farmacológico , Estrogênios não Esteroides/farmacocinética , Estrogênios não Esteroides/toxicidade , Feminino , Sistema Hipotálamo-Hipofisário/metabolismo , Isoflavonas/isolamento & purificação , Isoflavonas/farmacocinética , Isoflavonas/toxicidade , Lactação/metabolismo , Exposição Materna , Tamanho do Órgão/efeitos dos fármacos , Ovário/metabolismo , Fenóis/farmacocinética , Fenóis/toxicidade , Fitoestrógenos/isolamento & purificação , Fitoestrógenos/farmacocinética , Fitoestrógenos/toxicidade , Ratos , Ratos Sprague-Dawley , Maturidade Sexual/efeitos dos fármacos , Regulação para Cima , Útero/efeitos dos fármacos , Útero/metabolismo
20.
Biochem Biophys Res Commun ; 403(3-4): 398-404, 2010 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-21093411

RESUMO

Baicalin is a flavonoid compound purified from plant Scutellaria baicalensis Georgi. We aimed to evaluate the neuroprotective effects of baicalin against cerebral ischemic reperfusion injury. Male Wistar rats were subjected to middle cerebral artery occlusion (MCAO) for 2 h followed by reperfusion for 24 h. Baicalin at doses of 50, 100 and 200 mg/kg was intravenously injected after ischemia onset. Twenty-four hours after reperfusion, the neurological deficit was scored and infarct volume was measured. Hematoxylin and eosin (HE) staining was performed to analyze the histopathological changes of cortex and hippocampus neurons. We examined the levels of NF-κB p65 in ischemic cortexes by Western blot analysis and RT-PCR assay. The results showed that the neurological deficit scores were significantly decreased from 2.0 ± 0.7 to 1.2 ± 0.4 and the volume of infarction was reduced by 25% after baicalin injection. Histopathological examination showed that the increase of neurons with pycnotic shape and condensed nuclear in cortex and hippocampus were not observed in baicalin treated animals. Further examination showed that NF-κB p65 in cortex was increased after ischemia reperfusion injury, indicating the molecular mechanism of ischemia reperfusion injury. The level of NF-κB p65 was decreased by 73% after baicalin treatment. These results suggest that baicalin might be useful as a potential neuroprotective agent in stroke therapy. The neuroprotective effects of baicalin may relate to inhibition of NF-κB p65.


Assuntos
Isquemia Encefálica/tratamento farmacológico , Flavonoides/uso terapêutico , Infarto da Artéria Cerebral Média/complicações , Fármacos Neuroprotetores/uso terapêutico , Traumatismo por Reperfusão/tratamento farmacológico , Fator de Transcrição RelA/antagonistas & inibidores , Animais , Isquemia Encefálica/etiologia , Isquemia Encefálica/patologia , Modelos Animais de Doenças , Masculino , Ratos , Ratos Wistar , Traumatismo por Reperfusão/etiologia , Traumatismo por Reperfusão/patologia , Fator de Transcrição RelA/biossíntese
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