Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 290
Filtrar
1.
Artigo em Inglês | MEDLINE | ID: mdl-32371663

RESUMO

: To evaluate analytic and clinical performance of plasma thrombin-antithrombin complex (TAT) and D-dimer assay in assessing the severity and outcome of acute ischemic stroke. The prospective study was conducted and extended from January 2018 to December 2018. A total of 236 patients admitted within 24 h after neurologic symptoms onset were recruited. The median TAT and D-dimer levels were significantly higher in the acute ischemic stroke patients than in the controls. The average TAT levels in patients with mild, moderately severe and severe stroke were 1.75 [interquartile ranges (IQR), 1.1-2.6], 3.3 (IQR, 1.8-4.5) and 13.5 (IQR, 7.2-15.3) ng/ml. The D-dimer levels of respective patient groups were 0.39 (IQR, 0.22-0.73), 0.58 (IQR, 0.39-1.25) and 3.59 (IQR, 1.73-4.74) mg/l. With the optimal cut-off TAT level (1.75 ng/ml) determined from receiver operating characteristic analysis, the Area under the curve (AUC), the sensitivity and specificity of TAT for stroke diagnosis were 0.763, 58.1 and 87.8%. The cut-off D-dimer level was 0.38 mg/l and the AUC, the sensitivity and specificity were 0.772, 60.2 and 88.9%. The Area under the receiver operating characteristic curves (AUROCs) and sensitivity in the moderate to severe stroke increased to 0.903 and 86.9% for TAT, and 0.880 and 80.3% for D-dimer, respectively. Age and high TAT level were significant independent risk factors for stroke severity. Age, high initial National Institutes of Health Stroke Scale score and high TAT level were significant independent poor prognostic factors on multivariate analysis. TAT and D-dimer were superior in separating the moderate-to-severe stroke than mild stroke. A high TAT plasma level is an independent predictor for stroke severity and poor prognosis during 1-month follow-up.

2.
Cell Cycle ; : 1-10, 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32401588

RESUMO

BACKGROUND: Cerebral stroke refers to an acute onset of neurological deficit syndrome. In this research, we attempted to probe into the underlying mechanisms by which chrysophanol (CP) performed its regulatory roles in cerebral stroke. Methods OGD inducement was conducted in PC12 cells to construct a cerebral stroke model. Subsequently, CCK-8 assay, western blot, flow cytometry were utilized to determine cell viability, proliferation, and apoptosis, respectively. qRT-PCR was employed for detecting miR-216a expression level. Afterward, cell transfection was performed to alter miR-216a expression. Further, experiments were conducted to determine the expression of crucial factors participated in PI3 K/AKT and JAK2/STAT3 pathways for exploring the underlying mechanisms. Results OGD inducement suppressed cell viability, while promoted cell apoptosis. Besides, it enhanced the expression of proliferation-associated p53, p21, and apoptosis-associated Bax, and Cleaved-caspase-3, while suppressed the expression of Bcl-2. Furthermore, CHR exposure ameliorated the effects that OGD-evoked, and elevated the expression of miR-216a, as well as the expression of crucial factors participated in PI3 K/AKT and JAK2/STAT3 pathways. However, miR-216a silencing markedly reversed the effects triggered by CHR exposure. Conclusion CHR exposure relieved OGD-evoked PC12 cell damage by elevating miR-216a expression and thereby activating of PI3 K/AKT and JAK2/STAT3 pathways.

3.
Am J Ophthalmol ; 2020 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-32387430

RESUMO

PURPOSE: To compare the prediction accuracy of new intraocular lens (IOL) calculation formulas (Barrett Universal II (BUII), Emmetropia Verifying Optical (EVO), Kane and Ladas Super formula) and traditional formulas (Haigis, Hoffer Q, Holladay 1 and SRK/T) with Wang-Koch (WK) axial length (AL) adjustment in vitrectomized eyes. DESIGN: Retrospective consecutive case-series study. METHODS: 111 eyes of 111 patients underwent uneventful phacoemulsification and enVista MX60 implantation following vitrectomy were enrolled and divided into four groups according to whether the vitreous cavity was filled with silicone oil. The performance of each formula was evaluated with or without lens constant optimization. RESULTS: Before lens constants optimization, the mean prediction errors (MEs) of all formulas were statistically different from zero (0.14-0.46D) in vitrectomized eyes, except for the Kane formula. The BUII, EVO, Kane, and Haigis had relatively lower mean absolute error (MAE) and median absolute error (MedAE) with optimized constants. No significant systemic bias was found in new formulas for vitrectomized eyes with AL over 26 mm (P>0.05). The Hoffer Q and Holladay 1 displayed significantly hyperopic shift (0.39 and 0.51D) for long eyes, which was corrected by the WK adjustment. There were no significant differences in the prediction accuracy of all formulas among 4 subgroups (P>0.05). CONCLUSIONS: The BUII, EVO, Kane, and Haigis displayed comparable performance in vitrectomized eyes with optimized constants. In vitrectomized highly myopic eyes, the new formulas and traditional formulas with WK adjustment exhibited satisfactory prediction accuracy. Silicone oil tamponade did not affect the prediction accuracy of formulas using IOLMaster 700.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32352251

RESUMO

PURPOSE: To analyze the characteristics and factors associated with intraocular lens (IOL) tilt and decentration after uneventful phacoemulsification with IOL implantation. SETTING: Zhongshan Ophthalmic Centre, Sun Yat-sen University, Guangzhou, China. DESIGN: Cross-sectional study. METHODS: All patients underwent a general ophthalmologic examination and anterior segment photography. IOL tilt and decentration were measured with a second-generation anterior segment optical coherence tomography Casia2. Univariate and multivariate regression analyses were performed to assess the association between IOL tilt and decentration with ocular biometric and systemic parameters, and the visual acuity. RESULTS: IOLs showed an average tilt of 4.8 degrees towards the inferotemporal direction and the average decentration was 0.21 mm. Both eyes presented a mirror symmetry relationship. Twenty-two eyes (11.22%) had a tilt greater than 7 degrees, and 21 eyes (10.72%) had a decentration more than 0.4 mm. Multivariate regression analysis showed previous pars plana vitrectomy (PPV) and short axial length (AL) were associated with greater IOL tilt (P = 0.014 and P < 0.001). In addition, long AL, thicker lens and less capsulorhexis-IOL overlap were positively correlated with decentration (P < 0.001, P=0.029 and P=0.026). Corrected distance visual acuity did not directly correlate to IOL tilt and decentration (P=0.417 and P= 0.550). CONCLUSIONS: PPV history and short AL were associated with greater IOL tilt, while longer AL, thicker lens, and overlarge capsulorhexis contribute to greater decentration. Implantation of toric and multifocal IOLs in these patients should be cautious.

5.
Sci Rep ; 10(1): 6313, 2020 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-32286394

RESUMO

Salicylic acid (SA) is a key natural component that mediates local and systemic resistance to pathogens in many dicotyledonous species. However, its function is controversial in disease resistance in rice plants. Here, we show that the SA signaling is involved in both pathogen-associated-molecular-patterns triggered immunity (PTI) and effector triggered immunity (ETI) to Xanthomonas oryzae pv. Oryzae (Xoo) mediated by the recessive gene xa5, in which OsNPR3.3 plays an important role through interacting with TGAL11. Rice plants containing homozygous xa5 gene respond positively to exogenous SA, and their endogenous SA levels are also especially induced upon infection by the Xoo strain, PXO86. Depletion of endogenous SA can significantly attenuate plant resistance to PXO86, even to 86∆HrpXG (mutant PXO86 with a damaged type III secretion system). These results indicated that SA plays an important role in disease resistance in rice plants, which can be clouded by high levels of endogenous SA and the use of particular rice varieties.

6.
DNA Cell Biol ; 2020 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-32243216

RESUMO

Heterogeneity in patients with colorectal cancer (CRC) leads to different strategies in clinical decision making. Identifying distinctive subgroups in patients contributes to develop more individualized treatments. This study constructed a novel prediction model for the prognosis of CRC patients based on the value of risk score combining the expression status of immune-related genes and coefficients. In this study, we built an interactive network of prognosis-related immune genes and transcription factors and adopted several methods to verify the accuracy of model. Moreover, we assessed the correlation between risk score and immune infiltration. The results suggested that the model was well fit and the risk score could be an independent predictive factor for CRC patients. This model has high application value in the clinic.

7.
J Agric Food Chem ; 68(19): 5426-5436, 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32314918

RESUMO

In this study, we investigated the effect of high-molecular-weight glutenin subunits (HMW-GSs) on gluten quality and glutenin synthesis based on the cytological, physicochemical, and transcriptional levels using Xinong1718 and its three near-isogenic lines (NILs). Cytological observations showed that the endosperm of Glu-1Bh with Bx14+By15 accumulated more abundant and larger protein bodies at 10 and 16 days after anthesis than the other NILs. Glu-1Bh exhibited higher nitrogen metabolism enzyme gene expression and activity levels. The transcriptional levels of genes encoding HMW-GSs, protein folding, and transcription factors differed significantly among the NILs, and they were highest in Glu-1Bh. Our results demonstrate that variations in the expression patterns of nitrogen metabolism and glutenin synthesis-related genes may account for the differences in the accumulation of glutenin, glutenin macropolymers, and protein bodies, thereby affecting the structural and thermal stability of gluten. These findings provide novel insights into how different HMW-GSs might improve the quality of wheat.

8.
BMC Cancer ; 20(1): 287, 2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-32252671

RESUMO

BACKGROUND: The effects of dietary factors on prognosis of esophageal cancer remain unclear. This systematic review and meta-analysis aimed to assess the association between dietary intake and the risk of mortality among patients with esophageal cancer. METHODS: Six electronic databases (PubMed, Web of Science, OVID, ProQuest, CNKI and Wanfang) were searched for studies published up to Oct. 2019 that examined the association between dietary intake and all-cause mortality, esophageal cancer-specific mortality and esophageal cancer recurrence. The pooled hazard ratio (HR) with 95% confidence interval (CI) were derived by comparing the highest with the lowest categories of each dietary item and by using random effect models. RESULTS: A total of 15 cohort studies were included in this study and all reported pre-diagnosis dietary exposure; two focused on dietary folate, 12 on alcohol consumption and three on other dietary components (sugary beverages, phytochemicals and preserved vegetables). When comparing the highest with the lowest categories, dietary folate intake was associated with a reduced risk of esophageal cancer-specific mortality in patients with esophageal squamous cell carcinoma (HR: 0.41, 95% CI: 0.25-0.69), with low heterogeneity (I2 = 0%, P = 0.788). When comparing the highest with the lowest categories of alcohol consumption, alcohol consumption was associated with an increased risk of all-cause mortality in patients with esophageal squamous cell carcinoma (HR: 1.29, 95% CI: 1.07-1.55; heterogeneity: I2 = 53%, P = 0.030), but this increased risk was not significant in patients with esophageal adenocarcinoma (HR = 1.05, 95% CI: 0.84-1.32). CONCLUSIONS: This review with pre-diagnostic dietary exposure showed that dietary folate intake was associated with a reduced risk of mortality of esophageal squamous cell carcinoma, whereas alcohol consumption was associated with an increased risk. More studies are needed to investigate effect of dietary factors, especially post-diagnosis dietary consumption, on esophageal cancer prognosis.

9.
Br J Ophthalmol ; 2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32188679

RESUMO

PURPOSE: To investigate the prevalence of myopic macular degeneration (MMD) in the global population. METHODS: All published literature of population-based studies on MMD prevalence worldwide were searched and only those with clear definitions to diagnose and classify MMD lesions by standardised grading methods were selected. Meta-analysis methods were used to calculate the pooled prevalence of MMD and its 95% CI in a random-effects model. The prevalence of MMD lesions would also be reported, together with the subgroup analysis of age, region and gender. Correlation between MMD prevalence and spherical equivalent levels and axial length were also evaluated. RESULTS: 12 studies with 58 558 subjects were included in this meta-analysis. The pooled prevalence of MMD in the world population was 2.1% (95% CI: 1.3% to 3.3%). In the subgroup analysis, people with the following characteristics were at higher risk of developing MMD: female, urban life, living in Asia, older age, longer axial length and severer myopia. CONCLUSIONS: MMD is a serious public health concern worldwide, particularly in subjects who are women, subjects living in urban areas, subjects living in Asia, and subjects with longer axial lengths and severer myopia. Further studies from other continents/ethnicities are needed for comprehensive estimates of the prevalence of MMD globally.

10.
Nat Commun ; 11(1): 1366, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32170064

RESUMO

In Arabidopsis, a zygote undergoes asymmetrical cell division that establishes the first two distinct cell types of early proembryos, apical and basal cells. However, the genome-wide transcriptional activities that guide divergence of apical and basal cell development remain unknown. Here, we present a comprehensive transcriptome analysis of apical and basal cell lineages, uncovering distinct molecular pathways during cell lineage specification. Selective deletion of inherited transcripts and specific de novo transcription contribute to the establishment of cell lineage-specific pathways for cell fate specification. Embryo-related pathways have been specifically activated in apical cell lineage since 1-cell embryo stage, but quick transcriptome remodeling toward suspensor-specific pathways are found in basal cell lineage. Furthermore, long noncoding RNAs and alternative splicing isoforms may be involved in cell lineage specification. This work also provides a valuable lineage-specific transcriptome resource to elucidate the molecular pathways for divergence of apical and basal cell lineages at genome-wide scale.

11.
Foods ; 9(3)2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32197430

RESUMO

Wheat gluten properties can be improved by the application of nitrogen. This study investigates the effects of nitrogen application in the booting stage on glutenin polymerization during grain-filling and structural-thermal properties of gluten based on the high-molecular-weight glutenin subunits (HMW-GSs) using near-isogenic lines (Glu-1Da and Glu-1Dd). The nitrogen rate experiment included rates of 0, 60, 90, and 120 kg N ha-1 applied with three replicates. Nitrogen significantly improved the grain quality traits (wet gluten contents, Zeleny sedimentation values, and maximum resistance) and dough strength (dough development time, dough stability time, and protein weakening), especially in wheat with the Glu-1Da allele. Nitrogen increased the protein composition contents, proportions of glutenins and HMW-GSs, and disulfide bond concentration in the flours of Glu-1Da and Glu-1Dd, and accelerated the polymerization of glutenins (appearing as glutenin macropolymer) during grain-filling, where nitrogen enhanced the accumulation and polymerization of glutenins more for line containing Glu-1Da than Glu-1Dd. The ß-sheets, α-helix/ß-sheet ratio, microstructures, and thermal stability were also improved to a greater degree by nitrogen for gluten with Glu-1Da compared to Glu-1Dd. Nitrogen treatment was highly effective at improving the gluten structural‒thermal properties of wheat in the booting stage, especially with inferior glutenin subunits.

12.
Virology ; 544: 1-11, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32174509

RESUMO

The hepatitis E virus (HEV) ORF2 truncated recombinant proteins can self-assemble into virus-like particles (VLPs) and were used as models to investigate the HEV capsid assembly. However, the structural function of the ORF2 C-terminal domain (C52aa from aa 608 to aa 660) remains unclear. Herein, by analyzing a set of ORF2 truncated proteins expressed in Escherichia coli, we found that the highly conserved C-terminal cysteines play a crucial role in the oligomerization of the truncated ORF2 proteins and in their assembly into VLPs, through the formation of dimer-dimer disulfide bonds; and the treatment of native HEV particles with dithiothreitol (DTT) induced the disassembly of the viral capsid, suggesting that the disulfide bonding is required for stabilizing the native HEV capsid. The present study sheds light on the structural role of the C-terminal region of the HEV capsid protein and contributes to the full understating of the viral capsid assembly process.

13.
J Matern Fetal Neonatal Med ; : 1-7, 2020 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-32212945

RESUMO

Objective: To compare the clinical and ultrasound characteristics of cesarean scar pregnancy (CSP) and other lower uterine segment pregnancies (LUSPs) to enable differential diagnosis of the two pathologies in the first trimester.Methods: A retrospective analysis of early pregnancies with a gestational sac located in the lower uterine segment with cesarean section history in our hospital from December 2011 to February 2018 was performed. According to the diagnoses, the patients were divided into the CSPs group and the other LUSPs group. The clinical and ultrasound features were compared.Results: A total of 38 patients were enrolled with 16 (42.1%) CSPs and 22 (57.9%) other LUSPs. Compared to the other LUSPs group, the CSPs group had thinner lower uterine myometrium (0.16 ± 0.11 cm versus 0.32 ± 0.14 cm, p = .000), larger maximum diameter of the gestational sac (5.14 ± 2.08 cm versus 3.77 ± 1.24 cm, p = .028); the distribution of the villous chorion sites (p = .001) and the main blood supply sites (p = .000) of the gestational sac were different between the two groups. Surgical bleeding volume during the termination of gestation was higher in the CSPs group than in the other LUSPs group (p = .028).Conclusion: Patients with CSPs and other LUSPs have different imaging characteristics on ultrasound. The prognosis of these patients also differs. Ultrasound feature is very useful in the differential diagnosis of these two clinical-mimicking pathologies.

14.
Food Res Int ; 130: 108950, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32156392

RESUMO

To further understand the gluten-starch interactions in dough, this study investigated the effects of exogenous starch on the structural-thermal properties of gluten via reconstituting Xinong 836 starch with gluten from near-isogenic lines HMW-D1a (Dy12) and HMW-D1p (Dx2 + Dy12) according to the following proportions (gluten/starch): 9/91 (G09), 12/88 (G12), 15/85 (G15), and two controls, where G00 and G01 represent the original and self-reconstituted flours, respectively. Adding exogenous starch significantly improved the dough strength for the reconstituted flours containing gluten from HMW-D1a and HMW-D1p, especially those with HMW-D1a. When the ratios of gluten to starch were 15/85 and 12/88 in the HMW-D1a and HMW-D1p reconstituted flours, respectively, the concentrations of free sulfhydryl groups were minimized in the flour, and thus more glutenin polymers were formed. Changes in the secondary structure, such as the proportion of ß-sheets and the α-helices differed among the gluten types after adding starch. Compared with G00, the microstructures of dough obtained from G15 with HMW-D1a and G12 with HMW-D1p were more compact and denser, respectively; the thermal stability was also improved significantly for G15 with HMW-D1a and G12 with HMW-D1p. The effects of adding starch on the gluten characteristics were greater in HMW-D1a than HMW-D1p, attributing to differences in gluten-starch interactions. These findings indicate that adding exogenous starch was more readily to modify the structural properties of HMW-D1a gluten with inferior subunits, which helps to improve the quality of dough prepared with inferior gluten.

15.
Cyberpsychol Behav Soc Netw ; 23(5): 303-311, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32191529

RESUMO

Prolonged mobile phone use (MPU) is prevalent in adolescents. This study examined the associations between prolonged MPU and academic performance in Chinese adolescents. Participants were 11,831 adolescents who participated in the Shandong Adolescent Behavior and Health Cohort in 2015. We used a self-administered questionnaire to collect data on demographics, weekday and weekend MPU, sleep duration, insomnia, depression, and academic performance. We obtained a subsample's year-end achievement test scores for Chinese, Mathematics, and English, 3 months after baseline survey (n = 856). Results showed that with prolonged MPU from ≥1 hour/day on weekdays and ≥2 hours/day on weekends, the prevalence of self-reported poor academic performance significantly increased, and the achievement test scores significantly decreased. After controlling for adolescent and family covariates, students who used mobile phone ≥2 hours/day on weekdays (OR = 2.12, 95% CI = 1.82-2.47) and ≥5 hours/day on weekends (OR = 1.50, 95% CI = 1.31-1.72) were significantly more likely to report poor overall academic performance and scored significantly lower on Mathematics and English compared with those who used mobile phone <1 hour/day on weekdays and <2 hours/day on weekends, respectively. The mediating effects of reduced sleep duration, insomnia, and depression on the association between prolonged MPU and academic performance was small. In conclusion, prolonged MPU is associated with poor academic performance as measured by self-reports and subject tests in Chinese adolescents. Our findings suggest that adolescents should be advised to limit the time of MPU to minimize its harmful effects on sleep, mental health, and academic performance.

16.
Int J Mol Sci ; 21(4)2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-32085665

RESUMO

Absence of high-molecular-weight glutenin subunit (HMW-GS) Dx2 weakens the gluten quality, but it is unclear how the absence of Dx2 has these effects. Thus, we investigated the gluten quality in terms of cytological, physicochemical, and transcriptional characteristics using two near-isogenic lines with Dx2 absent or present at Glu-D1 locus. Cytological observations showed that absence of Dx2 delayed and decreased the accumulation of protein bodies (PBs), where fewer and smaller PBs formed in the endosperm. The activity and gene expression levels of nitrogen assimilation and proteolysis enzymes were lower in HMW-D1a without Dx2 than HMW-D1p with Dx2, and thus less amino acid was transported for protein synthesis in the grains. The expression pattern of genes encoding Glu-1Dx2+1Dy12 was similar to those of three transcription factors, where these genes were significantly down-regulated in HMW-D1a than HMW-D1p. Three genes involving with glutenin polymerization were also down-regulated in HMW-D1a. These results may explain the changes in the glutenin and glutenin macropolymer (GMP) levels during grain development. Therefore, we suggest that the lower nitrogen metabolism capacity and expression levels of glutenin synthesis-related genes in HMW-D1a accounted for the lower accumulation of glutenin, GMP, and PBs, thereby weakening the structural‒thermal properties of gluten.

17.
Bioorg Chem ; 97: 103648, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32065882

RESUMO

Natural protoberberine alkaloids were first identified and characterized as potent, selective and cellular active lysine specific demethylase 1 (LSD1) inhibitors. Due to our study, isoquinoline-based tetracyclic scaffold was identified as the key structural element for their anti-LSD1 activity, subtle changes of substituents attached to the core structure led to dramatic changes of the activity. Among these protoberberine alkaloids, epiberberine potently inhibited LSD1 (IC50 = 0.14 ± 0.01 µM) and was highly selective to LSD1 over MAO-A/B. Furthermore, epiberberine could induce the expression of CD86, CD11b and CD14 in THP-1 and HL-60 cells, confirming its cellular activity of inducing acute myeloid leukemia (AML) cells differentiation. Moreover, epiberberine prolonged the survival of THP-1 cells bearing mice and inhibited the growth of AML cells in vivo without obvious global toxicity. These findings give the potential application of epiberberine in AML treatment, and the isoquinoline-based tetracyclic scaffold could be used for further development of LSD1 inhibitors.

18.
Neurosci Lett ; 723: 134861, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32105765

RESUMO

Ototoxic drugs may induce auditory sensory hair cell loss and permanent deafness; however, there is still no effective treatments or prevention strategies for this side effect. A recent study found that microRNA182 (miR-182) protected cochlear hair cells from ototoxic drug-induced apoptosis in vitro. However, it remains unclear whether miR-182 can protect drug-induced deafness in vivo. In this study, we overexpressed cochlear miR-182 in Sprague-Dawley rats by trans-round window niche delivery of miR-182 mimics. The rats subsequently received intraperitoneal injections of kanamycin and furosemide to induce acute cochlear outer hair cell death and permanent deafness. Auditory brainstem response tests showed that miR-182 attenuated permanent threshold shifts. Consistent with this result, miR-182 reduced the loss of outer hair cells and missing stereocilia. miR-182 treatment also increased the level of phosphoinositide-3 kinase regulatory subunit p85α in the outer hair cells after co-administration of kanamycin and furosemide. Our findings suggest that miR-182 has powerful protective potential against ototoxic drug-induced acute auditory sensory hair cell loss and permanent deafness.

19.
Lasers Med Sci ; 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32026165

RESUMO

Cadmium (Cd) is one of the toxic heavy metals which is confirmed to be related to male sterile. Here, confocal Raman spectroscopy was employed to detect biomolecular composition and changes in testis under acute and chronic Cd treatment. Specific Raman shifts associated with mitochondria, nucleic acids, proteins, lipids, and cholesterol were identified which were distinguishing among groups undergoing different Cd treatment times. Supporting evidences were provided by conventional experimental detections. The relevant biochemical parameters, pathological changes, and protein expression related to testosterone synthesis were all changed and consistent with Raman spectrum information. In conclusion, confocal Raman spectroscopy presents a reliable data and provides a novel method which is expected to be a promising strategy in reproduction toxicity research.

20.
Nanoscale ; 12(14): 7586-7594, 2020 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-32057066

RESUMO

Hybrid capacitive deionization (HCDI) has emerged as a promising desalination technique due to its ultra-high salt removal capacity in high brine water. However, the mechanism behind HCDI is seldom discussed anywhere. Herein, we perform a comprehensive investigation to have some insight into the HCDI behavior of NaxCoO2 by varying x as 0.2, 0.5, 0.7, 1.0 and 1.6. Regardless of x, NaxCoO2 are classified as a representative P63/mmc space group with a P2 layered structure. With the increase of the sodium content, the (002) crystal plane of NaxCoO2 shifts significantly toward a high angle as the distance between CoO2 layers decreases. This results from the variation of the Na-O bonding length as well as the bonding energy according to the first-principles simulation. Moreover, it is observed that the Na-O bond broke once the input energy is higher than the Na-O bonding energy, leading to the electrochemical pre-activation of NaxCoO2. As a result, Na0.7CoO2 exhibits the best HCDI performance, i.e. a salt removal capacity of 63.0 mg g-1 and a charge efficiency of 97% in NaCl solutions with an initial conductivity of 2000 µS cm-1. Besides, the intercalation of sodium ions into NaxCoO2 has been confirmed by differentiating the respective contributions of pseudo-capacitance together with crystal phase transformation. Our results show that the desalination behavior of NaxCoO2 can be mediated by controlling the sodium content and electrochemical pre-activation.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA