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1.
Zhongguo Gu Shang ; 32(6): 531-534, 2019 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-31277536

RESUMO

OBJECTIVE: To study the predictive value of procalcitonin as a serum biomarker in diagnosis of late periprosthetic joint infection(PJI) for providing theoretical reference basis for diagnosis of PJI. METHODS: A total of 77 cases were retrospective included from January 2015 to December 2017 for revision of total hip arthroplasty and total knee arthroplasty, according to the diagnostic criteria of Musculoskeletal Infection Society(MISI). All cases were divided into infection group and non-infection group. Infection group included 21 cases, 7 cases for male and 14 cases for female, with an average age of (60.70±8.75) years old (ranged 43 to 75 years old). Non-infection group 56 included cases, 24 cases for male and 32 cases for female, with an average age (64.40±12.14) years old (ranged 43 to 85 years old). Concentration of preoperative serum procalcitonin was examined and the chi-square test was used to compare positive rate between the two groups. RESULTS: Two cases in the infection group had positive serum procalcitonin, 0.06 ng/L and 0.10 ng/L respectively, with the positive rate of 9.52%; 4 cases in non-infected group had positive serum procalcitonin, 0.05 ng/L, 0.06 ng/L, 0.06 ng/L, 0.16 ng/L respectively, with the positive rate of 7.14%. No statistically significant difference was observed between the two groups (P=0.662). CONCLUSIONS: Most of PJI were low toxicity infection with little systemic inflammatory response, so the concentration of serum procalcitonin was normal or slightly higher level, had little clinical significance for diagnosis of PJI. But the cases of this retrospective study are not enough, more cases are needed for further study.


Assuntos
Artroplastia de Quadril , Artroplastia do Joelho , Infecções Relacionadas à Prótese , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pró-Calcitonina , Estudos Retrospectivos
2.
BMC Plant Biol ; 19(1): 205, 2019 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-31109303

RESUMO

BACKGROUND: Fall dormancy and freezing tolerance characterized as two important phenotypic traits, have great effects on productivity and persistence of alfalfa (Medicago sativa L.). Despite the fact that one of the most limiting traits for alfalfa freezing tolerance in winter is fall dormancy, the interplay between fall dormancy and cold acclimation processes of alfalfa remains largely unknown. We compared the plant regrowth, winter survival, raffinose and amino acids accumulation, and genome-wide differentially expressed genes of fall-dormant cultivar with non-dormant cultivar under cold acclimation. RESULTS: Averaged over both years, the non-dormant alfalfa exhibited largely rapid regrowth compared with fall dormant alfalfa after last cutting in autumn, but the winter survival rate of fall dormant alfalfa was about 34-fold higher than that of non-dormant alfalfa. The accumulation of raffinose and amino acids were significantly increased in fall dormant alfalfa, whereas were decreased in non-dormant alfalfa under cold acclimation. Expressions of candidate genes encoding raffinose biosynthesis genes were highly up-regulated in fall dormant alfalfa, but down-regulated in non-dormant alfalfa under cold acclimation. In fall dormant alfalfa, there was a significantly down-regulated expression of candidate genes encoding the glutamine synthase, which is indirectly involved in the proline metabolism. A total of eight significantly differentially expressed transcription factors (TFs) related to CBF and ABRE-BFs were identified. The most up-regulated TFs in fall dormant alfalfa cultivar were ABF4 and DREB1C. CONCLUSIONS: Fall dormant alfalfa drastically increased raffinose and amino acids accumulation under cold acclimation. Raffinose-associated and amino acid-associated genes involved in metabolic pathways were more highly expressed in fall dormant alfalfa than non-dormant alfalfa under cold acclimation. This global survey of transcriptome profiles provides new insights into the interplay between fall dormancy and cold acclimation in alfalfa.


Assuntos
Regulação da Expressão Gênica de Plantas/fisiologia , Medicago sativa/fisiologia , Dormência de Plantas/genética , Proteínas de Plantas/genética , Transcriptoma/fisiologia , Perfilação da Expressão Gênica , Longevidade , Medicago sativa/genética , Estações do Ano
3.
Huan Jing Ke Xue ; 40(3): 1261-1269, 2019 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-31087973

RESUMO

Denitrification is the most important nitrogen removal process in lake waters, and is of great significance for mitigating nitrogen pollution and controlling eutrophication in lakes. The outbreak and decline of cyanobacterial blooms may promote denitrification directly in the water column by changing the nitrogen circulation pathway and microenvironment of the water body, and accelerate the removal of nitrogen. In order to verify this hypothesis, the cyanobacteria with different biomass and the NO3--N, PO43--P nutrient for 10 days were taken from Taihu Lake water to simulate the effects of cyanobacteria growth and degradation on denitrification. The dynamic changes of algal biomass and various forms of nitrogen concentration were simultaneously determined by 15N isotope addition culture combined with membrane inlet mass spectrometer (MIMS) for real-time quantitative determination of denitrification rate. The results showed that cyanobacteria absorbed nitrogen into particle nitrogen during the growth period. During the decay period, algae cells released a large amount of NH4+-N by degrading mineralization, which was then converted into NO3--N to provide a substrate for denitrification. That is the key to promoting denitrification in water; the denitrification rate (as N2) reaches (1614.52±301.57)µmol·(m2·h)-1, which is three times higher than the denitrification rate[ (534.45±242.18)µmol·(m2·h)-1]of the lowest concentration cyanobacterial group at the same time. At the end of the experiment, the highest rate of TN removal was highest in the group with the highest initial biomass of cyanobacteria (40.02%), which was 2.26 times of the TN removal rate (17.72%) in the control, indicating that cyanobacterial accumulation can significantly promote the intensity of denitrification and accelerate the removal of nitrogen in water. The rate of denitrification in the decline of cyanobacteria is significantly affected by the concentration of NH4+-N, indicating that the coupling of nitrification-denitrification of microorganisms attached to algae is the main route of nitrogen removal. The results study indicate that the cyanobacteria bloom rapidly during the growth period. Nitrogen is converted into particle nitrogen. The degradation of cyanobacteria is accelerated by coupled nitrification-denitrification, which may be one of the reasons for the decrease of nitrogen concentration in Taihu Lake.

4.
Mol Cell ; 70(2): 340-357.e8, 2018 04 19.
Artigo em Inglês | MEDLINE | ID: mdl-29628309

RESUMO

Whereas the actions of enhancers in gene transcriptional regulation are well established, roles of JmjC-domain-containing proteins in mediating enhancer activation remain poorly understood. Here, we report that recruitment of the JmjC-domain-containing protein 6 (JMJD6) to estrogen receptor alpha (ERα)-bound active enhancers is required for RNA polymerase II recruitment and enhancer RNA production on enhancers, resulting in transcriptional pause release of cognate estrogen target genes. JMJD6 is found to interact with MED12 in the mediator complex to regulate its recruitment. Unexpectedly, JMJD6 is necessary for MED12 to interact with CARM1, which methylates MED12 at multiple arginine sites and regulates its chromatin binding. Consistent with its role in transcriptional activation, JMJD6 is required for estrogen/ERα-induced breast cancer cell growth and tumorigenesis. Our data have uncovered a critical regulator of estrogen/ERα-induced enhancer coding gene activation and breast cancer cell potency, providing a potential therapeutic target of ER-positive breast cancers.


Assuntos
Neoplasias da Mama/enzimologia , Proliferação de Células , Receptor alfa de Estrogênio/metabolismo , Histona Desmetilases com o Domínio Jumonji/metabolismo , Complexo Mediador/metabolismo , Proteína-Arginina N-Metiltransferases/metabolismo , Ativação Transcricional , Animais , Sítios de Ligação , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Proliferação de Células/efeitos dos fármacos , Estradiol/farmacologia , Receptor alfa de Estrogênio/agonistas , Receptor alfa de Estrogênio/genética , Feminino , Regulação Enzimológica da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Células HEK293 , Humanos , Histona Desmetilases com o Domínio Jumonji/genética , Células MCF-7 , Complexo Mediador/genética , Camundongos Endogâmicos BALB C , Camundongos Nus , Ligação Proteica , Transporte Proteico , Proteína-Arginina N-Metiltransferases/genética , Transdução de Sinais , Ativação Transcricional/efeitos dos fármacos
5.
Appl Opt ; 57(5): 983-991, 2018 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-29469878

RESUMO

A triple reflection grazing incidence x-ray telescope is proposed and evaluated. This form of an optical system can detect x-ray energy that is close to the optical axis, which solves the problems encountered by traditional Wolter-type systems. In this paper, we also propose a new design method to ensure that the entire telescope structure is compact and integrated. Finally, a proof-of-concept design with an acceptable image quality is proposed.

6.
Opt Express ; 25(22): 27691-27705, 2017 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-29092240

RESUMO

We propose a new panossramic optical system that provides an additional field of view (FOV) channel without expanding the physical size of a conventional panoramic annular lens (PAL). The two channels are contained within one PAL, their optical paths do not interfere with each other, and the two images are realized on a single image plane. A prototype panoramic lens was developed that provides a 360° × (38-80°) front FOV channel and a 360° × (102-140°) back FOV channel.

7.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 48(3): 460-463, 2017 May.
Artigo em Chinês | MEDLINE | ID: mdl-28616926

RESUMO

OBJECTIVES: To apply chromosomal microarray analysis (CMA) in the diagnosis of karyotyping with uncertain genomic rearrangement. METHODS: We retrospectively reviewed 48 samples (34 samples of amniotic fluid, 14 samples of peripheral blood) of karyotype analyses with uncertain genomic rearrangement in patients admitted to our department from September 2014 to April 2016. The CMA results were compared with those of karyotyping. RESULTS: The 48 samples consisted of 13 samples with marker chromosomes, 19 samples with derivative chromosomes, and 16 samples with balanced translocation. Sixteen cases (33.33%) were detected with abnormalities by CMA. In the 32 samples with marker chromosomes or derivative chromosomes, 16 cases were detected with deletions or duplications (>5 Mb) by CMA, including 1 case 21-trisomy, 2 cases XYY syndrome and 3 cases microdeletion/ microduplication syndromes (22q11 duplication syndrome, Wolf-Hirschhorn syndrome and 15q26 overgrowth syndrome). In the 16 balanced translocation cases, all revealed negative results in CMA. CONCLUSIONS: CMA can confirm the karyotyping with uncertain genomic rearrangement and clarify its clinical significance.


Assuntos
Transtornos Cromossômicos/diagnóstico , Rearranjo Gênico , Cariotipagem , Análise em Microsséries , Diagnóstico Pré-Natal , Feminino , Genoma Humano , Humanos , Gravidez , Estudos Retrospectivos
8.
Huan Jing Ke Xue ; 38(5): 1947-1956, 2017 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965100

RESUMO

A novel composite adsorbent (Ti-Zr-D201) for simultaneous removal of phosphate and fluoride from water was prepared by loading nanosized titanium and zirconium oxides on the anion exchange resin named D201. Combining with the characterization of the adsorbent, adsorption isotherm experiments, effect of solution pH experiments, competitive tests, kinetic experiments and fixed bed column adsorption experiments were performed to explore the adsorption performance and mechanism. The maximum adsorption capacity of Ti-Zr-D201 for phosphorus and fluorine was 34.9mg·g-1 and 35.1mg·g-1 respectively, when the pH value was 5.8 and the temperature was 308K. Adsorption behavior was spontaneous, and higher temperature was favorable for phosphorus and fluoride adsorption. The effect of pH on the adsorption of fluoride was more significant compared with the adsorption of phosphorus. SO42-, NO3- and Cl- were selected as the competitive ions for competition experiments, and the results indicated that Ti-Zr-D201 exhibited favorable sorption selectivity for phosphorus and fluoride compared with the host material D201. The fitting results of the internal diffusion model showed that there were two different adsorption stages before the adsorption equilibrium of Ti-Zr-D201. Column adsorption experiments showed that Ti-Zr-D201 had a stable structure, excellent dynamic adsorption performance, and could be recycled, which showed the potential of practical application.

9.
Arch Gerontol Geriatr ; 62: 143-51, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26466781

RESUMO

OBJECTIVE: The issue of non-response in dementia epidemiological studies, which may result in the underestimation of the prevalence of dementia, has attracted little attention. We aimed to explore the causes and related factors of non-response in a dementia survey among Chinese veterans. METHODS: A two-phase, cross-sectional study investigated the prevalence of dementia and mild cognitive impairment in Chinese veterans aged ≥ 60 years. We collected the socio-demographic data and prior medical history, evaluated the health status of veterans and their caregivers, assessed the cognitive status of veterans, and evaluated the care burden of caregivers by Caregiver Burden Inventory (CBI). RESULTS: Of 9676 eligible participants, 525 (5.4%) veterans in phase 1 and 1706 (35.0%) veterans among 4875 veterans in phase 2 did not respond. Illness, hospitalization and death accounted for 63.0% and 75.5% non-response in phases 1 and 2, respectively. Non-participation in social activities, self-perceived poor health status, worsened health changes, self-reported need for life care, and history of hearing loss or glaucoma independently predicted non-response in phase 1 or 2. The heavy care burden, suggested by the higher CBI scores and self-reported health deterioration of the primary caregivers, predicted non-response in phase 1 or 2. CONCLUSIONS: The negative factors from both the participants and their caregivers independently predicted the non-response in the dementia study in an older population. Preventative strategies from the perspectives of the participants and caregivers should be developed to improve the response rates in both phases in a cross-sectional study.


Assuntos
Cuidadores/psicologia , Efeitos Psicossociais da Doença , Demência/psicologia , Nível de Saúde , Veteranos/psicologia , Adaptação Psicológica , Idoso , Cuidadores/estatística & dados numéricos , Cognição , Disfunção Cognitiva/epidemiologia , Estudos Transversais , Demência/epidemiologia , Feminino , Hospitalização/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Atividade Motora , Prevalência , Autorrelato , Fatores Socioeconômicos , Inquéritos e Questionários , Veteranos/estatística & dados numéricos
10.
Atherosclerosis ; 235(1): 102-9, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24942709

RESUMO

OBJECTIVES: Low vitamin D status has been shown to be associated with hypertension. We planned to research the effect of vitamin D and nifedipine in the treatment of patients with essential hypertension. METHODS: Patients with grades I-II essential hypertension were enrolled in this single-center, double-blind, placebo-controlled trial in Beijing. All patients received a conventional antihypertensive drug (nifedipine, 30 mg/d). One hundred and twenty-six patients were randomly assigned to receive vitamin D (n=63, 2000 IU/d) or a placebo (n=63) as an add-on to nifedipine, by the method of permutated block randomization. Ambulatory blood pressure monitoring was performed at baseline (month 0), at month 3 and at month 6. RESULTS: In vitamin D supplementation group, there was a significant increase in mean 25-hydroxyvitamin D levels from baseline (19.4 ± 11.6 ng/ml) to 6 months (34.1 ± 12.2 ng/ml; p<0.001). At 6 months, the primary end points, a difference in the fall of 24-h mean blood pressure, between the groups was -6.2 mmHg (95% CI -11.2; -1.1) for systolic blood pressure (p<0.001) and -4.2 mmHg (95% CI -8.8; -0.3) for diastolic blood pressure (p<0.001) under intention to treat analysis. In patients with vitamin D <30 ng/ml at baseline (n=113), 24-h mean blood pressure decreased by 7.1/5.7 mmHg (p<0.001). Safety and tolerability were similar among the two groups. CONCLUSIONS: Vitamin D supplementation can reduce blood pressure in patients with hypertension, it can be an adjuvant therapy for patients with grades I-II essential hypertension. CLINICAL TRIAL REGISTRATION: This study was registered in the Chinese Clinical Trial Registry, it is available in Website: http://www.chictr.org/cn/; REGISTRATION NUMBER: ChiCTR-ONC-13003840.


Assuntos
Hipertensão/tratamento farmacológico , Nifedipino/uso terapêutico , Vitamina D/uso terapêutico , Idoso , Pressão Sanguínea , Proteína C-Reativa/metabolismo , China , Suplementos Nutricionais , Método Duplo-Cego , Hipertensão Essencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Nifedipino/administração & dosagem , Reprodutibilidade dos Testes , Resultado do Tratamento
11.
Ann Noninvasive Electrocardiol ; 19(2): 166-73, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24206389

RESUMO

BACKGROUND: Low vitamin D status has been associated with increased risk of cardiovascular disease. Atrial fibrillation (AF) is the most common cardiac arrhythmia. We evaluated the association between low vitamin D and AF. METHODS: We analyzed data from 162 Chinese patients with nonvalvular persistent AF and no other cardiovascular disease whose serum 25-hydroxyvitamin D [25(OH)D] levels were measured in our hospital (AF group). Healthy subjects without AF who underwent health screening at our hospital served as controls (non-AF group, n = 160). 25(OH)D was measured by chemiluminescence assay. RESULTS: The serum 25(OH)D level was significantly lower in the AF group than in the non-AF group (18.5 ± 10.3 vs 21.4 ± 10.7 ng/mL, P < 0.05). The high-sensitivity C-reactive protein (hsCRP) level was significantly higher in the AF group than in the non-AF group (0.35 ± 0.19 vs 0.2 ± 0.17 mg/dL, P < 0.01). The average left atrial diameter was significantly larger in the AF group than in the non-AF group (P < 0.01). The serum 25(OH)D level showed a negative correlation with left atrial diameter, hsCRP level, and pulmonary artery systolic pressure. Logistic regression analysis identified that 25(OH)D was related to AF. Patients whose vitamin D levels were in the lowest 25(OH)D category (<20 ng/mL) were more often in the AF group, with their incidence about twofold higher than those in the highest 25(OH)D category (>30 ng/mL). CONCLUSIONS: Low vitamin D levels are associated with AF. It may be involved in its development.


Assuntos
Fibrilação Atrial/sangue , Vitamina D/análogos & derivados , Idoso , Fibrilação Atrial/fisiopatologia , Pressão Sanguínea/fisiologia , Proteína C-Reativa , China , Feminino , Átrios do Coração/diagnóstico por imagem , Átrios do Coração/fisiopatologia , Humanos , Medições Luminescentes/métodos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Ultrassonografia , Vitamina D/sangue
13.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 43(1): 15-8, 2012 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-22455123

RESUMO

OBJECTIVE: To establish in vitro culture procedure for human amniotic fluid-derived CD117 positive stem cells, and to identify the characteristics of CD117 positive stem cells. METHODS: 86 amniotic fluid samples (10 mL of each) were obtained by second-trimester amniocentesis. Isolation of amniotic fluid-derived stem cells expressing CD117 antigen was performed via magnetic cell sorting using the CD117 MicroBead Kit. The karyotype of CD117 positive stem cells was analysed through repeated freezing. Adipogenic differentiation of these CD117 positive stem cells was displayed by Oil Red O staining. Osteogeneic differentiation of these CD117 positive stem cells was confirmed by Alizarin Red staining. RESULTS: The CD117 positive stem cells were successfully isolated and cultured from 61 samples, with all showing normal karyotype. Product analysis of specific staining confirmed that under specific culture mediums, these cells could be successfully induced to differentiate into adipocytes and osteocytes. CONCLUSION: Based on this study, we estimate that isolating CD117 positive stem cells from second-trimester amniotic fluid obtained by amniocentesis has a success rate of 70.93%. These cells maintain morphological and genetic stability in vitro. Human amniotic fluid-derived CD117 positive stem cells have the ability to differentiate in vitro into adipocytes and osteocytes under specific culture mediums and may be applied in cell transplantation and regenerative medicine.


Assuntos
Líquido Amniótico/citologia , Técnicas de Cultura de Células/métodos , Diferenciação Celular/fisiologia , Células-Tronco/citologia , Adipócitos/citologia , Adolescente , Adulto , Separação Celular , Células Cultivadas , Feminino , Humanos , Osteoblastos/citologia , Gravidez , Segundo Trimestre da Gravidez , Proteínas Proto-Oncogênicas c-kit/metabolismo , Adulto Jovem
14.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 41(2): 320-3, 2010 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-20506663

RESUMO

OBJECTIVE: To study the epidemiological and clinical features of congenital split hand/split foot malformation (SHFM) in Chinese population. METHODS: Data used in this study were provided by the Chinese Birth Defects Monitoring Network. The SHFM cases were categorized into two groups: isolated (SHFM only and SHFM with other limb defects) and syndromic (SHFM with non-limb defects). Prevalence rates were calculated by residential area (urban versus rural) and by gender. Further analyses were conducted to identify the characteristics of SHFM with related to gestational age, birth weight, perinatal outcome and affected limbs. RESULTS: A total of 736 newborn babies were identified with SHFM among 4,489,692 births, with a prevalence of 1.64/10,000. The prevalence of isolated SHFM and syndromic SHFM were 0.64 and 1.00 per 10,000 births, respectively. The prevalence of SHFM in male and female babies were 1.79 and 1.25 per 10,000 births, respectively. The prevalence of SHFM in urban and rural areas were 1.51 and 1.86 per 10,000 births, respectively. Preterm birth and low birth weight accounted for 30.20% and 43.93% of the SHFM cases, respectively. The perinatal mortality for the SHFM, isolated SHFM, and syndromic SHFM were 50.27%, 24.74%, and 66.59%, respectively. SHFM occurred more often in upper limbs. CONCLUSION: The prevalence of SHFM in Chinese population is greater than foreign populations. The high perinatal mortality rate of SHFM is associated with the severity of accompanied malformations.


Assuntos
Deformidades Congênitas do Pé/epidemiologia , Deformidades Congênitas da Mão/epidemiologia , Anormalidades Múltiplas/epidemiologia , Anormalidades Múltiplas/genética , Adulto , China/epidemiologia , Feminino , Deformidades Congênitas do Pé/genética , Deformidades Congênitas da Mão/genética , Humanos , Recém-Nascido , Masculino , Gravidez , Prevalência
15.
Artigo em Chinês | MEDLINE | ID: mdl-19567043

RESUMO

OBJECTIVE: To explore the experience and value of overlay tympanoplasty. METHODS: Sixty-three ears with overlay tympanoplasty were reviewed and followed up for the external auditory canal, tympanic membrane and hearing. RESULTS: The diseases of the patients included middle ear cholesteatoma in 25 ears and chronic suppurative otitis media in 38 ears. The surgical techniques involved three kinds: overlay tympanoplasty, overlay tympanoplasty with canal wall up mastoidectomy and overlay tympanoplasty with canal wall down mastoidectomy. In middle ear cholesteatoma and suppurative otitis media patients, the case received the three techniques are 4, 17, 4 ears and 19, 18, 1 ears respectively. All patients gained stage I incision cure. Followed up for 0.5 to 3.5 years respectively, the external auditory canal was wide and tympanic membrane gained a good shape. The hearing in all case kept intact or increased while hearing decrease did not occur. Complications were free in patients with punctual visit. CONCLUSIONS: Overlay tympanoplasty has positive significance in treating the chronic otitis media with the merits of standard procedure, sufficient operative field and thorough erosion elimination.


Assuntos
Colesteatoma da Orelha Média/cirurgia , Otite Média Supurativa/cirurgia , Timpanoplastia/métodos , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento , Adulto Jovem
16.
Artigo em Chinês | MEDLINE | ID: mdl-17633818

RESUMO

OBJECTIVE: To study the effect of 6 formulations of adjuvants on immunoprotection in mice induced by the recombinant Ts87 protein (rTs87) of Trichinella spiralis. METHODS: ICR mice were vaccinated subcutaneously 3 times in 2-week intervals with rTs87 and different adjuvants CFA or IFA, Montanide IMS1312, Montanide ISA720, Quil-A and AI(OH)3, respectively. The antibody response was detected by ELISA and the purified rTs87 was analyzed by SDS-PAGE and Western blot. The protection induced in mice was then evaluated by muscle larvae reduction after Trichinella spiralis challenge. RESULTS: The results clearly showed that all the 6 adjuvants were able to enhance specific anti-rTs87 IgG response. SDS-PAGE and Western blot revealed that the molecular weight of expressed protein was around Mr 40 000 and could be recognized by sera from mice infected with Trichinella spiralis and mice immunized with rTs87. The reduction rate of muscle larvae was 49.4%, 49.2%, 63.5%, 65.1% and 70.8%, respectively. CONCLUSION: Protective immunity of rTs87 can be enhanced by adjuvant CFA or IFA, IMS1312, ISA720, Quil-A and Al(OH)3, and better effect is observed with the latter 3 adjuvants.


Assuntos
Adjuvantes Imunológicos/farmacologia , Imunoglobulina G/análise , Proteínas Recombinantes/imunologia , Trichinella spiralis/imunologia , Hidróxido de Alumínio/farmacologia , Animais , Ensaio de Imunoadsorção Enzimática , Feminino , Imunização , Larva/efeitos dos fármacos , Larva/imunologia , Camundongos , Camundongos Endogâmicos ICR , Saponinas de Quilaia , Saponinas/farmacologia , Triquinelose/imunologia , Triquinelose/parasitologia , Triquinelose/prevenção & controle
17.
Zhonghua Yi Xue Za Zhi ; 87(46): 3292-4, 2007 Dec 11.
Artigo em Chinês | MEDLINE | ID: mdl-18396628

RESUMO

OBJECTIVE: To study the genotypic resistance profiles of HIV-1 children failing highly active antiretroviral therapy (HAART) so as to provide helpful information for the treatment regime of Chinese children infected with HIV-1. METHODS: Peripheral venous blood samples were collected from 20 HIV-1 infected children of Henan province, aged 9 (3 - 14). Nested RT-PCR was used to amplify part of the RT (40 -250 aa) gene. The PCR products of RT gene underwent nucleotide sequencing, the resulting nucleotide sequences were analyzed by the HIVdb data offered by the Stanford University web site to find the drug resistance mutations. RESULTS: (1) Phylogenetic analysis revealed that 20 of the RT sequences were classified as subtype B. (2) According to the genotypic analysis, 20 , 15, and 13 children showed high level resistance to the nevirapine. (NVP), delavirdine (DLV), and efavirenz (EFV) respectively; 7 and 5 children showed high and intermediate level resistance to azidothymidine (AZT) respectively. Five children showed potential low-level and intermediate level resistance to lamivudine (3TC), and 11 showed high level resistance to 3TC; 11 showed intermediate and high level resistance to stavudine (d4T) and didanoside (ddI) respectively; and 19 and 12 children showed resistance to abacavir (ABC) and tenofovir (TDF) which had never been taken by these children. CONCLUSION: The emergence of HIV resistant strains during antiretroviral therapy is one of the main reasons for treatment failure in HIV-infected children.


Assuntos
Terapia Antirretroviral de Alta Atividade , HIV-1/efeitos dos fármacos , HIV-1/genética , Síndrome de Imunodeficiência Adquirida/tratamento farmacológico , Síndrome de Imunodeficiência Adquirida/virologia , Adolescente , Fármacos Anti-HIV/farmacologia , Fármacos Anti-HIV/uso terapêutico , Benzoxazinas/farmacologia , Benzoxazinas/uso terapêutico , Criança , Pré-Escolar , China , Delavirdina/farmacologia , Delavirdina/uso terapêutico , Farmacorresistência Viral/genética , Genótipo , Humanos , Mutação , Nevirapina/farmacologia , Nevirapina/uso terapêutico , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Carga Viral , Zidovudina/farmacologia , Zidovudina/uso terapêutico
18.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 28(5): 658-61, 2006 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-17121226

RESUMO

OBJECTIVE: To explore the impacts of traditional Chinese medicine (TCM) on CD4 + T cell counts and human immunodeficiency virus (HIV) viral loads during the course of structured treatment interruption (STI) in highly active antiretroviral therapy (HAART). METHODS: Nineteen HIV/ADIS patients were treated for 14 months as follows: initiated with zidovudine/lamivudine + efavirdine for 6 months, then discontinued the therapy and treated with TCM instead for 2 months. HAART was then reinitiated for another 3 months, and then discontinued and replaced with TCM for another 3 months. The changes of CD4 + T cell counts and HIV viral loads were measured. RESULTS: During the first STI of HAART, 43.8% of patients had no viral rebounds one month later, and 62.6% had stable or increased immune functions; 18.8% had no viral rebounds two months later, and 43.8% had stable or increased immune functions. Changes of viral loads were not significantly different between these two months (P = 0.097), while CD4 + T cell counts significantly decreased two months later compared with one month later (P = 0.043). During the second STI of HAART, 33.3% of patients had no viral rebounds one month later, and 64.3% had stable or increased immune functions; 13.3% had no viral rebounds 3 months later and 46.6% had stable or increased immune functions. Changes of viral loads had significant difference (P = 0. 017), while CD4 + T cell counts at month 12 elevated significantly compared with the baseline (P = 0.014). CONCLUSIONS: TCM can suppress the viral rebounds during STI-HAART, maintain immune functions. However, this effect may decrease along with the prolongation of STI-HAART.


Assuntos
Fármacos Anti-HIV/uso terapêutico , Medicamentos de Ervas Chinesas/uso terapêutico , Infecções por HIV/tratamento farmacológico , Fitoterapia , Adulto , Fármacos Anti-HIV/administração & dosagem , Terapia Antirretroviral de Alta Atividade , Benzoxazinas/uso terapêutico , Contagem de Linfócito CD4 , Quimioterapia Combinada , Feminino , Seguimentos , Infecções por HIV/imunologia , Infecções por HIV/virologia , Humanos , Lamivudina/uso terapêutico , Masculino , Pessoa de Meia-Idade , Fatores de Tempo , Resultado do Tratamento , Carga Viral , Zidovudina/uso terapêutico
20.
Zhonghua Yi Xue Za Zhi ; 85(43): 3079-83, 2005 Nov 16.
Artigo em Chinês | MEDLINE | ID: mdl-16324412

RESUMO

OBJECTIVE: To investigate the effects of small interfering RNA (siRNA) targeting the polymerase (P) gene sequence of hepatitis B virus (HBV) on the replication and antigen secretion of HBV. METHODS: From the 29 base sequences of the HBV in the HepG2.2.15 cells that accord with the demands of siRNA designing five sequences targeting the P gene of HBV were selected and cloned into the siRNA expressing vector pGE-1. Then the plasmid pGE-HBVP was transfected into the cultured HepG2.2.15 cells. Chemiluminescent immunoassay was used to determine the levels of HBsAg and HBeAg in the supernatant of culture medium 24, 48, 72, and 96 hours after the transfection and the expression of HBsAg in the 2.2.15 cells 24 hours after the transfection so as to observe the inhibitory effects. Untransfected cells and cells transfected with blank pGE-1 vector were used as controls. RESULTS: Five vectors expressing the siRNAs targeting the HBV P region, pGE-HBVP1-pGE-HBV5 were successfully constructed. The efficiency of transfection of the vectors into the 2.2.15 cells were 30% to 40%. 24, 48, 72, and 96 hours after the transfection of pGE-HBVP2, the strongest inhibitor among the five, the inhibitory rates of HBsAg secretion in the supernatant were 28.88%, 32.28%, 29.10%, and 18.42% respectively; and the inhibitory rates of HBeAg secretion were 38.33%, 27.50%, 33.41%, and 12.60% respectively. In view of the transfection efficiency of 30%-40%, the actual inhibitory rate of HBV antigen secretion might reach 80% and over. 24 hours after the transfection the expression rate of HBsAg in the 2.2.15 cells transfected with pGE-HBVP2 was 50%, significantly lower than that in the cells transfected with the blank vector pGE-1 (82%). CONCLUSION: siRNAs targeting the HBV P gene effectively prevent the HBV gene expression and replication and may play an important role in the clinical anti-viral treatment.


Assuntos
Produtos do Gene pol/metabolismo , Antígenos de Superfície da Hepatite B/metabolismo , Antígenos E da Hepatite B/metabolismo , Interferência de RNA , Plasmídeos de Bacteriocinas/genética , Linhagem Celular Tumoral , Produtos do Gene pol/genética , Vírus da Hepatite B/enzimologia , Vírus da Hepatite B/genética , Vírus da Hepatite B/imunologia , Humanos , Imunoensaio , Transfecção
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