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1.
Food Microbiol ; 86: 103347, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31703852

RESUMO

Gutian Hong Qu glutinous rice wine (GHQW) is a representative alcoholic beverage in China. During its traditional brewing process, multiple strains are involved. However, the roles of these microorganisms, particularly their contributions to aroma formation, are poorly understood. Therefore, in this study, the dynamics of volatile components and microbial succession during the traditional fermentation process of GHQW were monitored, by using HS-SPME-GC/MS and high throughput 16 S rRNA and ITS sequencing techniques. Following a 32-day semi-open solid-state fermentation, massive changes in volatile profile and microbial communities were observed. Based on these, the potential correlations between volatile components and microorganisms during the fermentation were explored by using Pearson correlation analysis. It was revealed that 3 bacterial taxa, including Lactococcus lactis, Burkholderia gladioli and Cronobacter helveticus, and 5 fungal taxa, including Wickerhamomyces anomalus, Saccharomyces cerevisiae, Aspergillus vitricola, Aspergillus penicillioides and Monascus purpureus, showed strong connections with various volatile components. Thus these bacteria and fungi might play crucial roles in volatile components biosynthesis, and thus were preliminary considered as core functional microorganisms for GHQW brewing. The detailed information on the dynamic changes of volatile components and microbial communities throughout GHQW brewing can further expand our understanding of the formation mechanisms of GHQW aroma.

2.
Zhen Ci Yan Jiu ; 44(7): 501-5, 2019 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-31368281

RESUMO

OBJECTIVE: To observe the effect of electroacupuncture (EA) on expression of 5-HT1A receptor (5-HT1AR) and c-fos proteins in the hypothalamus and colon tissues in functional diarrhea (FD) rats so as to explore its underlying mechanisms in improving intestinal function via brain-gut axis. METHODS: A total of 40 SD rats were randomized into blank control, model, EA Tianshu (ST25)-Dachangshu (BL25, ST25-BL25) and EA Quchi (LI11)- Shangjuxu (ST37, LI11-ST37) groups. The FD model was established by gavage of Folium Sennae (10 mg/kg) and constraint immobilization once daily for 29 days. EA (10 Hz/50 Hz, 1.5 mA) was applied to bilateral ST25 and BL25 in EA ST25-BL25 group, and bilateral LI11 and ST37 in EA LI11-ST37 group for 20 min, once daily for successive 10 days. The expression of 5-HT1AR and c-fos protein in the hypothalamus and colon tissues was determined by using Western blot. The state of stool was recorded for calculating the loose stool rate and diarrhea index. RESULTS: After modeling, the loose stool rate, diarrhea index and expression levels of 5-HT1AR and c-fos proteins in the colon and hypothalamus tissues were obviously increased in the model group in contrast with the blank control group(P<0.01,P<0.05). Following EA intervention, the loose stool rate and diarrhea index, the expression levels of 5-HT1AR and c-fos proteins in the hypothalamus and colon in the EA ST25-BL25 group, and the expression of 5-HT1AR in the colon in the EA LI11-ST37 group were significantly down-regulated relevant to the model group (P<0.01,P<0.05). No significant changes were found in loose stool rate and diarrhea index,and the expression levels of hypothalamic 5-HT1AR and hypothalamic and colonic c-fos proteins in the EA LI11-ST37 group (P>0.05). The expression levels of 5-HT1AR protein in the hypothalamus and c-fos protein in the hypothalamus and colon in the EA LI11-ST37 group were significantly up-regulated relevant to the EA ST25-BL25 group (P<0.05). CONCLUSION: EA of ST25-BL25 can down-regulate expression of 5-HT1AR and c-fos proteins in the hypothalamus and colon tissue in FD rats, which may contribute to its function in improving symptoms of FD possibly via brain-gut axis.


Assuntos
Eletroacupuntura , Pontos de Acupuntura , Animais , Encéfalo , Colo , Diarreia , Hipotálamo , Proteínas Proto-Oncogênicas c-fos , Ratos , Ratos Sprague-Dawley , Serotonina
4.
Phys Med Biol ; 64(14): 145010, 2019 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-31170699

RESUMO

This study aims to utilize a deep convolutional neural network (DCNN) for synthesized CT image generation based on cone-beam CT (CBCT) and to apply the images to dose calculations for nasopharyngeal carcinoma (NPC). An encoder-decoder 2D U-Net neural network was produced. A total of 70 CBCT/CT paired images of NPC cancer patients were used for training (50), validation (10) and testing (10) datasets. The testing datasets were treated with the same prescription dose (70 Gy to PTVnx70, 68 Gy to PTVnd68, 62 Gy to the PTV62 and 54 Gy to the PTV54). The mean error (ME) and mean absolute error (MAE) for the true CT images were calculated for image quality evaluation of the synthesized CT. The dose-volume histogram (DVH) dose metric difference and 3D gamma pass rate for the true CT images were calculated for dose analysis, and the results were compared with those for the CBCT images (original CBCT images without any correction) and a patient-specific calibration (PSC) method. Compared with CBCT, the range of the MAE for synthesized CT images improved from (60, 120) to (6, 27) Hounsfield units (HU), and the ME improved from (-74, 51) to (-26, 4) HU. Compared with the true CT method, the average DVH dose metric differences for the CBCT, PSC and synthesized CT methods were 0.8% ± 1.9%, 0.4% ± 0.7% and 0.2% ± 0.6%, respectively. The 1%/1 mm gamma pass rates within the body for the CBCT, PSC and synthesized CT methods were 90.8% ± 6.2%, 94.1% ± 4.4% and 95.5% ± 1.6%, respectively, and the rates within the PTVnx70 were 80.3% ± 16.6%, 87.9% ± 19.7%, 98.6% ± 2.9%, respectively. The DCNN model can generate high-quality synthesized CT images from CBCT images and be used for accurate dose calculations for NPC patients. This finding has great significance for the clinical application of adaptive radiotherapy for NPC.

5.
J Am Chem Soc ; 141(23): 9197-9201, 2019 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-31145608

RESUMO

Identifying the intrinsic electrocatalytic activity of nanomaterials is challenging, as their characterization usually requires additives and binders whose contributions are difficult to dissect. Herein, we use nano impact electrochemistry as an additive-free method to overcome this problem. Due to the efficient mass transport at individual catalyst nanoparticles, high current densities can be realized. High-resolution bright-field transmission electron microscopy and selected area diffraction studies of the catalyst particles before and after the experiments provide valuable insights in the transformation of the nanomaterials during harsh oxygen evolution reaction (OER) conditions. We demonstrate this for 4 nm sized CoFe2O4 spinel nanoparticles. It is revealed that these particles retain their size and crystal structure even after OER at current densities as high as several kA·m-2. The steady-state current scales with the particle size distribution and is limited by the diffusion of produced oxygen away from the particle. This versatilely applicable method provides new insights into intrinsic nanocatalyst activities, which is key to the efficient development of improved and precious metal-free catalysts for renewable energy technologies.

6.
Int J Mol Sci ; 20(10)2019 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-31100788

RESUMO

Drought is a major limiting factor for plant growth and crop productivity. Many Calcineurin B-like interacting protein kinases (CIPKs) play crucial roles in plant adaptation to environmental stresses. It is particularly essential to find the phosphorylation targets of CIPKs and to study the underlying molecular mechanisms. In this study, we demonstrate that CIPK11 acts as a novel component to modulate drought stress in plants. The overexpression of CIPK11 (CIPK11OE) in Arabidopsis resulted in the decreased tolerance of plant to drought stress. When compared to wild type plants, CIPK11OE plants exhibited higher leaf water loss and higher content of reactive oxygen species (ROS) after drought treatment. Additionally, a yeast two hybrid screening assay by using CIPK11 as a bait captures Di19-3, a Cys2/His2-type zinc-finger transcription factor that is involved in drought stress, as a new interactor of CIPK11. Biochemical analysis revealed that CIPK11 interacted with Di19-3 in vivo and it was capable of phosphorylating Di19-3 in vitro. Genetic studies revealed that the function of CIPK11 in regulating drought stress was dependent on Di19-3. The transcripts of stress responsive genes, such as RAB18, RD29A, RD29B, and DREB2A were down-regulated in the CIPK11OE plants. Whereas overexpression of CIPK11 in di19-3 mutant background, expression levels of those marker genes were not significantly altered. Taken together, our results demonstrate that CIPK11 partly mediates the drought stress response by regulating the transcription factor Di19-3.


Assuntos
Adaptação Fisiológica/fisiologia , Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Secas , Proteínas Serina-Treonina Quinases/metabolismo , Estresse Fisiológico/fisiologia , Adaptação Fisiológica/genética , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Proteínas de Transporte/metabolismo , Proteínas e Peptídeos de Choque Frio/genética , Regulação da Expressão Gênica de Plantas , Folhas de Planta/metabolismo , Plantas Geneticamente Modificadas/genética , Proteínas Serina-Treonina Quinases/genética , Espécies Reativas de Oxigênio , Estresse Fisiológico/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Transcriptoma , Técnicas do Sistema de Duplo-Híbrido , Proteínas rab de Ligação ao GTP/genética
7.
Biomed Res Int ; 2019: 4690280, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31119171

RESUMO

Background/Aims: Alzheimer's disease (AD) is the most common neurodegenerative disease, and all researchers working in this field agree that oxidative stress is intimately associated with Alzheimer disease. In this study, we hypothesized that glutamine (Gln) offers protection against oxidative stress injury in SAMP8 mice as well as the underlying mechanism. Methods: The SAMP8 mice received glutamine intragastrically for 8 consecutive weeks to evaluate the protective effect of glutamine on oxidative stress in AD mice involving Wnt3a/ß-catenin signaling pathway. In addition, rat pheochromocytoma tumor cell line PC12 was pretreated with 32 µM glutamine for 2 h followed by 24 h incubation with 40 µM Aß25-35 to obtain in vitro data. Results: In vivo the administration of glutamine was found to ameliorate behavioral deficits and neuron damage, increase superoxide dismutase (SOD) and glutathione peroxidase (GSH-XP) activity, reduce the malondialdehyde (MDA) content, and activate the Wnt3a/ß-catenin signaling pathway in SAMP8 mice. In vitro glutamine treatment decreased the toxicity of Aß25-35 on PC12 cells and prevented apoptosis. Additionally, glutamine treatment increased SOD and GSH-XP activity and decreased MDA content and increased Wnt3a and ß-catenin protein levels. Interestingly, the DKK-1 (Wnt3a/ß-catenin pathway inhibitor) decreased the antioxidant capacity of glutamine in Aß25-35-treated PC12 cells. Conclusion: This study suggests that glutamine could protect against oxidative stress-induced injury in AD mice via the Wnt3a/ß-catenin signaling pathway.


Assuntos
Doença de Alzheimer/tratamento farmacológico , Glutamina/farmacologia , Proteína Wnt3A/genética , beta Catenina/genética , Doença de Alzheimer/genética , Doença de Alzheimer/patologia , Animais , Antioxidantes/farmacologia , Apoptose/efeitos dos fármacos , Modelos Animais de Doenças , Glutationa Peroxidase/genética , Humanos , Malondialdeído/metabolismo , Camundongos , Estresse Oxidativo/efeitos dos fármacos , Células PC12 , Ratos , Transdução de Sinais/efeitos dos fármacos , Superóxido Dismutase/genética
8.
Adv Mater ; 31(28): e1901241, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31125135

RESUMO

Defect engineering holds great promise for precise configuration of electrode materials for dramatically enhanced performance in the field of energy storage, but the high energy/large time cost and lack of control involved in this process represent a serious limit to its use. In response, a low-energy-cost and ultrafast universal converse voltage process is developed to effectively activate the capacitive performance of transition metal compounds integrated on carbon fiber paper, including Co-, Ni-, Mn-, Fe-, and Cr-based hybrids. As a representative example, this process triggers a phase conversion from cobalt hydroxide to electric-field-activated CoOOH (EA-CoOOH), leading to the formation of molecular structure with abundant defects, lattice disorders, and connecting holes, responsible for an enhanced performance within 10 min at room temperature. Moreover, the retained Co2+ in EA-CoOOH results in increased activity, confirmed by density functional theory calculations. Consequently, these EA-CoOOH hybrids deliver a capacitance value of 832 F g-1 at a current density of 1 A g-1 and exhibit a retention rate up to 78% (649 F g-1 ) at a super-large current density of 200 A g-1 . This technology paves a way for ultrafast configuration/modulation of defects on advanced materials toward application in the fields of energy and catalysis.

9.
Nat Commun ; 10(1): 1839, 2019 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-31015398

RESUMO

Hematopoietic stem and progenitor cells (HSPCs) are capable of producing all mature blood lineages, as well as maintaining the self-renewal ability throughout life. The hairy-like organelle, cilium, is present in most types of vertebrate cells, and plays important roles in various biological processes. However, it is unclear whether and how cilia regulate HSPC development in vertebrates. Here, we show that cilia-specific genes, involved in primary cilia formation and function, are required for HSPC development, especially in hemogenic endothelium (HE) specification in zebrafish embryos. Blocking primary cilia formation or function by genetic or chemical manipulations impairs HSPC development. Mechanistically, we uncover that primary cilia in endothelial cells transduce Notch signal to the earliest HE for proper HSPC specification during embryogenesis. Altogether, our findings reveal a pivotal role of endothelial primary cilia in HSPC development, and may shed lights into in vitro directed differentiation of HSPCs.


Assuntos
Cílios/metabolismo , Células-Tronco Hematopoéticas/fisiologia , Receptores Notch/metabolismo , Transdução de Sinais/fisiologia , Proteínas de Peixe-Zebra/metabolismo , Animais , Animais Geneticamente Modificados , Cílios/genética , Embrião não Mamífero , Desenvolvimento Embrionário/fisiologia , Hemangioblastos/citologia , Hemangioblastos/metabolismo , Hematopoese/fisiologia , Modelos Animais , Peixe-Zebra/fisiologia
10.
Small ; 15(18): e1901015, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30957431

RESUMO

Coordination tuning of catalysts is a highly effective strategy for activating and improving the intrinsic activity. Herein, a Co-engineered FeOOH catalyst integrated on carbon fiber paper (Co-FeOOH/CFP) is reported, which realized a great improvement of the oxygen evolution activity by tuning the coordination geometry of the Fe species with an electrochemically driven method. Experiments and theoretical calculation demonstrate that the FeO bonds of FeOOH are partially broken, which is rooted in the Co incorporation, thus resulting in unsaturated FeO6 ligand structures and a relatively narrow bandgap. Consequently, the reorganized Fe sites on the surface show an enhanced capability for adsorbing OH- species and the Co-FeOOH exhibits an improved conductivity. As expected, the Co-FeOOH/CFP hybrids exhibit an extremely low overpotential of ≈250 mV at 10 mA cm-2 and a small Tafel slope, which far outperforms that of electrochemically sluggish FeOOH. The present work emphasizes the importance of local Fe coordination in catalysis and provides an in-depth insight into the mechanism of the enhanced catalytic activity.

11.
Sci Rep ; 9(1): 3032, 2019 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-30816172

RESUMO

C-reactive protein (CRP) is widely used as a biomarker of inflammation. It plays important roles in innate immunity response as a member of pattern recognition receptors, by binding oxidation-specific epitopes including some intermediates of lipid oxidative chain reaction. The inferred antioxidative ability of CRP was ever demonstrated by only few in vitro evidences, and needs to be clarified especially in vivo. Herein, we expressed human CRP in three representative non-animal organisms (Escherichia coli, Saccharomyces cerevisiae, and tobacco) inherently lacking the milieu for CRP signalling, and found CRP did possess an intrinsic antioxidative ability. Heterologous CRP could confer increased oxidative resistance in its recombinant E. coli and yeast cells and transgenic tobaccos. We also revealed a positive correlation between the antioxidative effect of CRP and its solubility. Only soluble CRP could exhibit distinct antioxidative activity, while the CRP aggregates might be instead toxic (probably pro-oxidative) to cells. Moreover, fusion with hyper-acidic minipeptides could remarkably improve CRP solubility, and meanwhile guarantee or enhance CRP antioxidative ability. These results not only provide a new insight for understanding the etiology of CRP-involved inflammations and diseases, and also endorse a potential of CRP biotechnological applications in developing new pharmaceutical therapies and improving plant oxidative resistance.

12.
Medicine (Baltimore) ; 98(1): e13769, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30608387

RESUMO

This study aims to evaluate the effectiveness and safety of the application of a 3-dimensional (3D)-printed composite guide plate for atlantoaxial pedicle screw.This was a retrospective study. A total of 43 atlantoaxial dislocation patients admitted in our hospital between January 2014 and October 2016 were retrospectively analyzed. According to the different methods of operation, patients were divided into 2 groups: 3D-printed plate group (n = 19) and traditional fixation group (n = 24). Placement time, operation duration, fluoroscopy number, intraoperative bleeding volume, and the neck and shoulder pain visual analog scale and Japanese Orthopaedic Association cervical nerve function scores were compared between pre- and postoperation.Differences in general data between these 2 groups were not statistically significant (P > .05). For patients in the 3D-printed plate group, a total of 68 assisting screws were placed at the pedicle, the accuracy rate of screw placement was 94.1%, placement time was 2.2 ±â€Š0.4 minutes, fluoroscopy number was 4.6 ±â€Š1.1 times, operation duration was 197 ±â€Š41 minutes, and intraoperative bleeding volume was 395 ±â€Š64 mL. In the traditional fixation group, a total of 76 screws were placed at the pedicle of patients, the accuracy rate of screw placement was 76.3%, placement time was 3.4 ±â€Š0.7 minutes, fluoroscopy number was 9.4 ±â€Š2.7 times, operation duration was 245 ±â€Š67 minutes, and intraoperative bleeding volume was 552 ±â€Š79 mL. Differences in accuracy rate, placement time, fluoroscopy number, operation duration, and intraoperative bleeding volume between these 2 groups were statistically significant (P < .05).The effectiveness and safety of 3D-printed composite guide plate for atlantoaxial pedicle screw were better than traditional method.


Assuntos
Articulação Atlantoaxial/cirurgia , Placas Ósseas , Luxações Articulares/cirurgia , Parafusos Pediculares , Impressão Tridimensional , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
13.
Int J Mol Sci ; 20(2)2019 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-30658512

RESUMO

Abscisic acid (ABA) plays a fundamental role in plant growth and development, as well as in the responses to abiotic stresses. Previous studies have revealed that many components in ABA and drought stress signaling pathways are ubiquitinated by E3 ligases. In this study, AtPPRT1, a putative C3HC4 zinc-finger ubiquitin E3 ligase, was explored for its role in abiotic stress response in Arabidopsis thaliana. The expression of AtPPRT1 was induced by ABA. In addition, the ß-glucuronidase (GUS) gene driven by the AtPPRT1 promoter was more active in the root hair zone and root tips of primary and major lateral roots of young seedlings in the presence of ABA. The assays for seed germination, stomatal aperture, root length, and water deficit demonstrated that the AtPPRT1-overexpressing Arabidopsis was insensitive to ABA and sensitive to drought stress compared with wild-type (WT) plants. The analysis by quantitative real-time PCR (qRT-PCR) revealed that the expression of three stress-inducible genes (AtRAB18, AtERD10, and AtKIN1) were upregulated in the atpprt1 mutant and downregulated in AtPPRT1-overexpressing plants, while two ABA hydrolysis genes (AtCYP707A1 and AtCYP707A3) were downregulated in the atpprt1 mutant and upregulated in AtPPRT1-overexpressing plants in the presence of ABA. AtPPRT1 was localized in the mitochondria. Our findings indicate that AtPPRT1 plays a negative role in ABA and drought stress responses.


Assuntos
Arabidopsis/fisiologia , Secas , Regulação da Expressão Gênica de Plantas , Estresse Fisiológico , Transcriptoma , Ubiquitina-Proteína Ligases/genética , Ubiquitina-Proteína Ligases/metabolismo , Ácido Abscísico/metabolismo , Sequência de Aminoácidos , Proteínas de Arabidopsis/genética , Perfilação da Expressão Gênica , Hidrólise , Mitocôndrias/genética , Mitocôndrias/metabolismo , Mutação , Raízes de Plantas/genética , Raízes de Plantas/metabolismo , Domínios e Motivos de Interação entre Proteínas , Plântula , Ubiquitina-Proteína Ligases/química
14.
Environ Health Prev Med ; 24(1): 4, 2019 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-30611190

RESUMO

BACKGROUND: Parkinson's disease is a neurodegenerative disorder, and recent studies suggested that oxidative stress contributes to the degeneration of dopamine cell in Parkinson's disease. Glutamine also has a positive role in reducing oxidative stress damage. In this study, we hypothesized that glutamine offers protection against oxidative stress injury in 1-methyl-4-phenylpyridinium (MPP+)-induced Parkinson's disease cell model. METHODS: MPP+ was used to induce PD models in PC12 cells and classified into control, M0 (MPP+), G0 (glutamine), and M0+G0 groups. CCK-8 and AO/EB staining assays were used to examine cell proliferation and apoptosis, respectively. Western blotting was applied to examine the protein expression of PI3K, P-Akt, Akt, P-mTOR, and mTOR. RESULTS: We showed that glutamine suppressed cytotoxicity induced by MPP+ in PC12 cells. MPP+ decreased the superoxide dismutase and glutathione peroxidase activity and increased the malondialdehyde content, which were restored by glutamine. Moreover, MPP+ increased the expression of PI3K, P-Akt, Akt, P-mTOR, and mTOR, which were inhibited by glutamine. And the antioxidant capacity of glutamine on PC12 cells could be improved by LY294002 and inhibited by IGF-1. CONCLUSION: These results suggest that glutamine strengthens the antioxidant capacity in PC12 cells induced by MPP+ through inhibiting the activation of the PI3K/Akt signaling pathway. The effects of glutamine should be investigated and the protective mechanism of glutamine in PD must be explored in future studies.


Assuntos
Glutamina/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Fosfatidilinositol 3-Quinases/metabolismo , 1-Metil-4-fenilpiridínio/administração & dosagem , Análise de Variância , Animais , Técnicas de Cultura de Células , Modelos Animais de Doenças , Doença de Parkinson , Substâncias Protetoras/farmacologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos
15.
J Clin Epidemiol ; 105: 125-135, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30278212

RESUMO

OBJECTIVES: To examine, through a cross-sectional survey, how well safety information was reported among drug systematic reviews predating PRISMA harms checklist and explore factors associated with better reporting. STUDY DESIGN AND SETTING: We searched PubMed to identify all systematic reviews published in the Cochrane Database of Systematic Review or the core clinical journals in 2015, one year before the PRISMA harms checklist was published. We randomly selected, in a 1:1 ratio, Cochrane and non-Cochrane systematic reviews assessing drug effects (including both efficacy and safety). We used the PRISMA harms checklist published in 2016 to assess the quality of reporting of drug safety information. Multivariable linear regression analyses were used to explore the association of six prespecified variables with more complete reporting of PRISMA harms items. RESULTS: We included 120 systematic reviews, including 60 Cochrane and 60 non-Cochrane reviews. Scores on the PRISMA harms checklist (23 items) were low (median 4, [first, third quartile: 2, 6]), with no difference between Cochrane and non-Cochrane reviews (4.5 [2, 7] vs. 4 [2.5, 5]; P = 0.29). Among all eligible reviews, only one item (i.e., state conclusions in coherence with the review findings) was reported adequately (proportion of adherence 81.6%); proportion of reporting for other items ranged from 1.7% to 68.3%. The four essential reporting items from PRISMA harms checklist were also poorly complied (proportion of adherence ranged from 1.7% to 9.2%). Multivariable linear regression analyses found no significant associations between any study characteristic and reporting on the PRISMA harms, likely because of limited variability in scores across studies. CONCLUSIONS: The reporting of safety information was poor both for Cochrane and non-Cochrane drug systematic reviews predating PRISMA harms checklist. The findings suggested a strong need to use the PRISMA harms checklist for reporting safety among drug systematic reviews.

16.
J Mech Behav Biomed Mater ; 90: 604-614, 2018 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-30500698

RESUMO

Polyhedral oligomeric silsesquioxane reinforced poly (L-lactic acid) nanocomposites (PLLA/POSS) were prepared to overcome the insufficient mechanical properties of PLLA. In order to improve the compatibility between the nanofillers and matrix, PLLA chains were grafted onto the POSS nanoparticles via microwave-assisted ring opening polymerization (ROP). Herein, a series of interface-modified polyhedral oligomeric silsesquioxane (POSS-(PLLA)32) nanoparticles with various PLLA tail lengths were synthesized and the influence of the structure and additional amount of POSS nanoparticles on the properties of PLLA based nanocomposites were studied. POSS nanoparticles exhibit effective nucleation activity and lead to a significant improvement in the mechanical strength, thermal stability and biocompatibility of the resulting nanocomposites. The addition of 6 wt% POSS-(PLLA)32 600 shows the optimal mechanical properties owing to has the longest PLLA tail length on POSS core, which possesses the optimal interfacial compatibility between POSS nanoparticles and PLLA. The Young's modulus improved by 57% and the tensile strength increased by 26.5% compared with neat PLLA. Moreover, the introduction of POSS nanoparticles lead to a porous fiber structure when processed by electrospinning and the nanofibrous scaffold effectively promoted cells adhesion and spreading. These results demonstrate the potential applications of the PLLA/POSS nanocomposites in tissue engineering and regenerative medicine.

17.
Nat Commun ; 9(1): 5277, 2018 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-30538248

RESUMO

Defective ciliogenesis causes human developmental diseases termed ciliopathies. Microtubule (MT) asters originating from centrosomes in mitosis ensure the fidelity of cell division by positioning the spindle apparatus. However, the function of microtubule asters in interphase remains largely unknown. Here, we reveal an essential role of MT asters in transition zone (TZ) assembly during ciliogenesis. We demonstrate that the centrosome protein FSD1, whose biological function is largely unknown, anchors MT asters to interphase centrosomes by binding to microtubules. FSD1 knockdown causes defective ciliogenesis and affects embryonic development in vertebrates. We further show that disruption of MT aster anchorage by depleting FSD1 or other known anchoring proteins delocalizes the TZ assembly factor Cep290 from centriolar satellites, and causes TZ assembly defects. Thus, our study establishes FSD1 as a MT aster anchorage protein and reveals an important function of MT asters anchored by FSD1 in TZ assembly during ciliogenesis.


Assuntos
Axonema/metabolismo , Cílios/metabolismo , Microtúbulos/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Animais , Axonema/genética , Centrossomo/metabolismo , Cílios/genética , Humanos , Mitose , Proteínas do Tecido Nervoso/genética , Fuso Acromático/genética , Fuso Acromático/metabolismo , Peixe-Zebra/embriologia , Peixe-Zebra/genética , Peixe-Zebra/metabolismo
18.
Complement Ther Clin Pract ; 33: 197-203, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30396621

RESUMO

PURPOSE: To evaluate the methodological and reporting quality of systematic reviews (SRs) on acupuncture treatment for women with polycystic ovarian syndrome (PCOS). METHODS: A comprehensive search on multiple databases was performed. Methodological and reporting quality of reviews were assessed by revised assessment of multiple systematic reviews (AMSTAR 2) and preferred reporting items for SRs and meta-analyses (PRISMA), respectively. RESULTS: Ten SRs were included. Among the SRs using AMSTAR 2, two achieved a good overall rating (percentage of items with "yes" > 50%) and severe limitation existed in eleven items (percentage of items with "yes" < 50%). Among the SRs using PRISMA, six reviews achieved a good overall rating (percentage of items with "yes" > 50%), while twelve items were poorly reported (percentage of items with "yes" < 50%). CONCLUSION: There were many deficiencies in the methodological and reporting quality of SRs assessing acupuncture in women with PCOS.

19.
J Exp Bot ; 2018 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-30380101

RESUMO

Abscisic acid (ABA) plays a vital role in responses to abiotic stresses to mediate the environmental challenges. In this study, we analyzed ABA receptors of subfamily III as the potential targets of Cytosolic ABA Receptor Kinase 1 (CARK1). We previously found that CARK1 phosphorylated the subfamily III member RCAR11 at a distinct threonine residue (T78). Our study now shows the physical interaction of CARK1 with the receptors RCAR12/13/14 in vitro and in vivo. A catalytically inactive form of CARK1-N204A did not interact with the receptors. Phosphorylation of these ABA receptors in vitro occurred at a serine/threonine amino acid residue corresponding to T78 in RCAR11, which located in the loop of ß3 within a conserved site. Further analysis revealed that the phosphorylation of RCAR11T78 could increase the sensitivity of the pyr1pyl1pyl2pyl4 quadruple mutant (1124) toward ABA, including the inhibition of root elongation and increasing drought tolerance. The analysis of CARK1:1124 complementation and the expressions of ABA-related genes indicated that CARK1 could rescue the insensitivity of 1124 to ABA. Our results indicate that CARK1 tend to phosphorylate subfamily III ABA receptors and the phosphosites of RCAR11T78, RCAR12T105, RCAR13T101 and RCAR14S81 are the major sites involved in the activation of the ABA-response pathway.

20.
J Agric Food Chem ; 66(32): 8469-8477, 2018 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-30020786

RESUMO

Monomeric green tea catechin (GTC) and oligomeric, oxidized black tea phenolic (BTP) have shown promising health benefits, although GTC has been more extensively studied than BTP. We review the current knowledge on bioavailability, colonic transformation, and gut microbiota modulatory effects of GTC and BTP. As a result of their similar poor bioavailability in the small intestine and potentially similar metabolites upon colonic fermentation, it seems as if GTC and BTP have similar health effects, although it cannot be excluded that they have different gut microbiota modulatory effects and that BTP gives a poorer yield of bioactive phenolic metabolites upon colonic fermentation than GTC.


Assuntos
Bactérias/metabolismo , Camellia sinensis/metabolismo , Colo/microbiologia , Microbioma Gastrointestinal , Fenóis/metabolismo , Extratos Vegetais/metabolismo , Animais , Biotransformação , Camellia sinensis/química , Colo/metabolismo , Humanos , Fenóis/análise , Extratos Vegetais/análise
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