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1.
J Asian Nat Prod Res ; : 1-7, 2020 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-32228193

RESUMO

Two new pyrrolizidine alkaloids, sclerwalins A and B (1 and 2), and one known 9-O-E-hydroxysenecioylretronecine (3) were first isolated from the seeds of Scleropyrum wallichianum. Their chemical structures were elucidated by extensive 1 D NMR and 2 D NMR (HSQC, HMBC, COSY, and ROESY), MS and IR spectra. Cytotoxicities of all isolates were evaluated against five human tumor cell lines (HL-60, A-549, SMMC-7721, MCF-7 and SW480).

2.
EBioMedicine ; 53: 102689, 2020 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-32114396

RESUMO

BACKGROUND: How the oncoprotein epidermal growth factor receptor (EGFR) evades proteolytic degradation and accumulates in non-small cell lung cancer (NSCLC) remains unclear, and ubiquitin pathway genes (UPGs) that are critical to NSCLC needs to be systematically identified. METHODS: A total of 696 UPGs (including E1, E2, E3, and deubiquitinases) were silenced by small interfering RNA (siRNA) library in NSCLC cells, the candidates were verified, and their significance was evaluated in patients with NSCLC. The effects of a candidate gene on EGFR were investigated in vitro and in vivo. FINDINGS: We report 31 candidates that are required for cell proliferation, with the E2 ubiquitin conjugase CDC34 as the most significant one. CDC34 is elevated in tumor tissues in 76 of 114 (66.7%) NSCLCs and inversely associated with prognosis, is higher in smoker patients than nonsmoker patients, and is induced by tobacco carcinogens in normal human lung epithelial cells. Forced expression of CDC34 promotes, whereas knockdown of CDC34 inhibits, NSCLC cell proliferation in vitro and in vivo. CDC34 competes with c-Cbl to bind Y1045 to inhibit polyubiquitination and degradation of EGFR. In EGFR-L858R and EGFR-T790M/Del (exon 19)-driven lung tumor growth in mouse models, knockdown of CDC34 significantly inhibits tumor formation. INTERPRETATION: These results demonstrate that an E2 enzyme is capable of competing with E3 ligase to stabilize substrates, and CDC34 represents an attractive therapeutic target for NSCLCs. FUNDING: National Key Research and Development Program of China, National Natural Science Foundation of China, and the CAMS Innovation Fund for Medical Sciences.

3.
Microbiol Res ; 235: 126445, 2020 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-32113127

RESUMO

For the effective biocontrol of Syringa powdery mildew (Mircosphaera syringejaponicae) and to promote seedling growth, we identified 44 of the 181 Trichoderma isolates (T1-T181) isolated from the rhizosphere soil. Analysis identified 10 Trichoderma species, and T. pseudoharzianum T1 (TpseT1), T. afroharzianum T52 (TafrT52), and T. asperelloides T57 (TaspT57) were selected to make Trichoderma biofertilizer because of their fast growth and high spore production. Exposing Syringa oblata to Trichoderma biofertilizer showed that TafrT52 and TaspT57 could induce abscisic acid (ABA) production, and promote the shedding of diseased leaves and the generation of new leaves. Furthermore, TafrT52 increased the catalase (CAT) activity and reduced the H2O2 content. And the disease incidence was reduced by 37.84 % by Tasp (highest) in 2017 year and by 13.84 % by TpseT1(lowest) in 2018 year. In addition, all Trichoderma strains we selected could promote the lateral root growth of S. oblata seedlings; however, because of the downregulated gene expression at the late stage of chlorophyll synthesis, the chlorophyll content decreased in the new leaves. Antagonism among different Trichoderma species led to low biocontrol and growth promotion effects, thus the Trichoderma mixture cannot be use as biofertilizer. TafrT52, with better biocontrol and growth promotion effects, could be used for biocontrol of M. syringejaponicae.

4.
Cancer Lett ; 479: 100-111, 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32200035

RESUMO

Metastasis is still a major cause of cancer-related mortality. Lysosome-associated membrane protein 3 (LAMP3) has been implicated in the invasiveness and metastasis of multiple cancer types; however, the underlying mechanisms are unclear. In this study, we found that LAMP3 was overexpressed in esophageal squamous cell carcinoma (ESCC) tissues and that this increased expression positively correlated with lymph node metastasis. Depletion of LAMP3 dramatically suppressed the motility of ESCC cells in vitro and experimental pulmonary and lymph node metastasis in vivo. Importantly, knockdown of LAMP3 increased the level of phosphorylated VASP(Ser239), which attenuated the invasive and metastatic capability of ESCC cells. We identified that cAMP-dependent protein kinase A (PKA) was responsible for the phosphorylation of VASP at Ser239. Consistently, silencing of PKA regulatory subunits diminished Ser239 phosphorylation on VASP and restored the motility capacity of LAMP3-depleted ESCC cells. In conclusion, we uncovered a previously unknown role of LAMP3 in promoting cellular motility and metastasis in ESCC.

5.
Theranostics ; 10(8): 3594-3611, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32206110

RESUMO

Background: Accumulating evidences indicate that nanomedicines greatly decrease the side effects and enhance the efficacy of colorectal cancer (CRC) treatment. In particular, the use of rectal delivery of nanomedicines, with advantages such as fast therapeutic effects and a diminishing hepatic first-pass effect, is currently emerging. Method: We established a CRC targeted delivery system, in which α-lactalbumin peptosomes (PSs) co-loaded with a microRNA (miR)-31 inhibitor (miR-31i) and curcumin (Cur) were encapsuslated in thiolated TEMPO oxidized Konjac glucomannan (sOKGM) microspheres, referred as sOKGM-PS-miR-31i/Cur. The CRC targeting capability, drug release profiles, mucoadhesive-to-penetrating properties and therapeutic efficacy of sOKGM-PS-miR-31i/Cur delivery system were evaluated in colorectal cancer cells and azoxymethane-dextran sodium (AOM-DSS) induced tumor models. Results: sOKGM-PS-miR-31i/Cur delivery system were stable in the harsh gastrointestinal environment after rectal or oral administration; and were also mucoadhesive due to disulfide bond interactions with the colonic mucus layer, resulting in an enhanced drug retention and local bioavailability in the colon. Concomitantly, the released PS-miR-31i/Cur PSs from the microsphere was mucus-penetrating, efficiently passing through the colonic mucus layer, and allowed Cur and miR-31i specifically target to colon tumor cells with the guide of CD133 targeting peptides. Consequently, rectal delivery of sOKGM-PS-miR-31i/Cur microspheres suppressed tumor growth in an azoxymethane-dextran sodium sulfate (AOM-DSS)-induced tumor model. Conclusion: sOKGM-PS-miR-31i/Cur microspheres are effective rectal delivery system with combined advantages of mucoadhesive and mucus-penetrating properties, representing a potent and viable therapeutic approach for CRC.

6.
J Pharm Biomed Anal ; 182: 113127, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32058184

RESUMO

In order to identify real and fake Panax notoginseng samples, the high performance liquid chromatography (HPLC) was used to analyze P. notoginseng samples of non-volatile characteristic components in P. notoginseng powder samples with 10 %, 30 %, 50 % ratio, combined with principal component analysis - mahalanobis distance (PCA-MD). The results showed that: (1) The PCA analysis showed that mahalanobis with different stem and leaf powder were divided into four categories, and the gravity center of the pattern (classification) showed that the long axis of the trend chart gradually increased with the increase of the proportion of the stem and leaf powder, and the trend chart was gradually away from the control group; (2) The mahalanobis distance indicated that the range of mahalanobis distance fluctuation (upper limit) of several P. notoginseng powder samples is 395.03∼48252.70. The larger the percentage of the stem and leaf powder in P. notoginseng samples, it is farther away from the normal P. notoginseng samples. It indicated that the product of P. notoginseng samples can be evaluated with the mahalanobis distance. The results of this study can be applied to identification and homogenization evaluation of P. notoginseng between real and fake samples.

7.
J Sep Sci ; 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32034866

RESUMO

In order to determine six alkaloids (mass fraction) of nicotine, nornicotine, myosmine, anatabine, anabasine, and nicotyrine in tobacco and tobacco products quickly, accurately, and simultaneously, a novel method based on direct analysis of real-time model in situ ionization technique combined tandem mass spectrometry with a modified sample pretreatment was established, in which experimental parameters such as the type and amount of extraction solvent and injection rate were optimized, respectively. The samples of five commercial cigarettes and five kinds of tobacco leaves were analyzed by the established method, and the determined values were compared with those obtained using a gas chromatography with mass spectrometry method: (1) Under optimized conditions (30 mL ultrapure water as extraction solvent and with extraction rate of 0.6 mm/s), analysis could be completed within 10 min. (2) The linear range of the method was 0.002-2000 µg/g with R 2 = 0.9957 , the recovery ranged from 86.8 to 105.6%, and the limit of detection and the limit of quantification were 0.004-0.835 µg/g and 0.013-2.787 µg/g, respectively. (3) The relative standard deviation between direct analysis of real-time method and the gas chromatography with mass spectrometry method was 0.34-8.83%. The established method is rapid, reliable, and suitable for the ultrafast determination of six alkaloids in tobacco and tobacco products.

8.
Int J Cancer ; 2020 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-32064600

RESUMO

Although early detection and systemic therapies have improved the diagnosis and clinical cure rate of breast cancer, breast cancer remains the most frequently occurring malignant cancer in women due to a lack of sufficiently effective treatments. Thus, to develop potential targeted therapies and thus benefit more patients, it is helpful to understand how cancer cells work. ZIC family members have been shown to play important roles in neural development and carcinogenesis. In our study, we found that ZIC2 is downregulated in breast cancer tissues at both the mRNA and protein levels. Low expression of ZIC2 was correlated with poor outcome in breast cancer patients and serves as an independent prognostic marker. Furthermore, overexpression of ZIC2 repressed, whereas knockdown of ZIC2 promoted, cell proliferation and colony formation ability in vitro and tumor growth in vivo. Using ChIP-seq and RNA-seq analysis, we screened and identified STAT3 as a potential target for ZIC2. ZIC2 bound to the STAT3 promoter and repressed the promoter activities of STAT3. ZIC2 knockdown induced the expression of STAT3, increasing the level of phosphorylated STAT3. These results suggest that ZIC2 regulates the transcription of STAT3 by directly binding to the STAT3 promoter. Additionally, interfering STAT3 with siRNAs or inhibitors abrogated the oncogenic effects induced by decreased ZIC2. Taken together, our results indicate that ZIC2 serves as a useful prognostic marker in breast cancer and acts as a tumor suppressor by regulating STAT3, implying that STAT3 inhibitors might provide an alternative treatment option for breast cancer patients with ZIC2 downregulation.

9.
Cancer Epidemiol ; 65: 101682, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32036245

RESUMO

It has been reported that the number of children a mother has may impact on her concept of health and behavior, and there is a need to understand the role of children in the acceptability of human papillomavirus (HPV) testing and vaccination among Chinese mothers. A cross-sectional survey was conducted between January and June 2015 in Shenzhen, China, in which representative females were recruited from healthcare institutions through the Cervical Cancer Prevention Network. A total of 9058 females were included. Women with one child had a greater awareness of HPV (49.9 % versus 34.0 %, p < 0.001) and its vaccine (26.0 % versus 15.0 %, p <0.001), and were more likely to receive HPV testing (38.1 % versus 25.8 %, p <0.001) and vaccination (65.7 % versus 60.6 %, p <0.001) than those with two or more children. Mothers having one child who was a daughter were more likely to receive HPV testing (OR 1.53, 95 %CI 1.25-1.89) and HPV vaccination (OR 1.63, 95 %CI 1.38-1.93) than those having two or more children but without a daughter (p for interaction 0.014 and <0.001, respectively). Our findings provide a novel insight into cervical cancer prevention: a smaller number of children helps to improve a mother's awareness of HPV and its vaccine, to promote their practice for HPV testing, and to promote the acceptability of HPV vaccination. Having one or more daughters synergistically interacts with having fewer children in facilitating a mother's positive involvement in action against HPV infection.

10.
Cancer Res ; 80(3): 406-417, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32015157

RESUMO

Switch/Sucrose Non-Fermentable (SWI/SNF) chromatin-remodeling complexes have a mutation rate of approximately 20% in human cancer, and ARID1A is the most frequently mutated component. However, some components of SWI/SNF complexes, including ARID1A, exhibit a very low mutation rate in squamous cell carcinoma (SCC), and their role in SCC remains unknown. Here, we demonstrate that the low expression of ARID1A in SCC is the result of promoter hypermethylation. Low levels of ARID1A were associated with a poor prognosis. ARID1A maintained transcriptional homeostasis through both direct and indirect chromatin-remodeling mechanisms. Depletion of ARID1A activated an oncogenic transcriptome that drove SCC progression. The anti-inflammatory natural product parthenolide was synthetically lethal to ARID1A-depleted SCC cells due to its inhibition of both HDAC1 and oncogenic signaling. These findings support the clinical application of parthenolide to treat patients with SCC with low ARID1A expression. SIGNIFICANCE: This study reveals novel inactivation mechanisms and tumor-suppressive roles of ARID1A in SCC and proposes parthenolide as an effective treatment for patients with SCC with low ARID1A expression.

11.
Artigo em Inglês | MEDLINE | ID: mdl-32023074

RESUMO

Hippocampal network oscillations at gamma frequency band (γ oscillation, 20-80Hz) are synchronized synaptic activities generated by the interactions between the excitatory and inhibitory interneurons, and are associated with higher brain function such as learning and memory. Despite extensive studies about the modulation of intracellular kinases on synaptic transmission and plasticity, little is known about the effects of these kinases on γ oscillations. In this study, we examined the effects of several critical intracellular kinases such as cyclic-AMP-dependent protein kinase (PKA), protein protein kinase B (PKB)/Akt, protein kinase C (PKC), extracellular-regulated protein kinases (ERK) and AMP-activated protein kinase (AMPK), known to regulate synaptic transmission, on hippocam pal γ oscillations in vitro. We found that AMPK inhibitor but not PKA, PKC or ERK inhibitor, strongly enhanced the power of γ oscillation (γ power) and that Akt inhibitor weakly increased γ power. Western blot analysis confirmed that AMPK inhibitor reduced the expression of p-AMPK but not total AMPK. By using slice whole-cell voltage clamp technique, we found that AMPK inhibitor increased the frequency but not amplitude of spontaneous inhibitory postsynaptic currents (sIPSC) and had no effect on either frequency or amplitude of spontaneous excitatory postsynaptic currents (sEPSC). Therefore, AMPK activation negatively modulates hippocampal γ oscillation via modulation of the inhibitory neurons.

12.
Artigo em Inglês | MEDLINE | ID: mdl-32005617

RESUMO

BACKGROUND: Candida auris can form long-lasting colonies in the hospital environment and on human skin. There is limited evidence regarding the efficacy of different methods and products for disinfecting hospitals and colonized patients to prevent the spread of C. auris. METHODS: The minimum inhibitory concentration of three disinfectant products ("84" disinfectant, IodineTincture disinfectant, and quaternary ammonium) and 75% ethanol against C. auris and other Candida species were measured. A pig skin model was used to evaluate the efficacy of three hand hygiene products in killing pathogens. The killing effect of ultraviolet-C (253.7 nm) and the LK/CXD bed unit ozone disinfection machine on C. auris was also evaluated. RESULTS: Thirty seconds of pig skin washing with bacteriostatic hand sanitizer followed by drying and 15 s of ethanol-based gel can completely eradicate the colonization of C. auris (3.00 log10 CFU). The antifungal activity of ultraviolet-C to C. auris inoculated on bed sheets was significantly reduced (P < 0.01) at a distance of 1 m. Candida glabrata and C. auris showed greater resistance to ozone than other Candida species. The ozone could completely eradicate C. auris (3.60 log10 CFU) on bed sheets at dosage of 300 mg/m3 for 40 min of exposure. CONCLUSIONS: We recommend extending the disinfection times of ultraviolet-C and ozone and emphasizing the effectiveness of washing skin with soap, drying skin, and then applying an ethanol-based gel to remove C. auris from skin.

13.
Chemosphere ; 243: 125428, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31995880

RESUMO

Toxic elements exposure disturbs the homeostasis of essential elements in organisms, but the mechanism remains elusive. In this study, we demonstrated that Drosophila melanogaster exposed to Lead (Pb, a pervasive environmental threat to human health) exhibited various health defects, including retarded development, decreased survival rate, impaired mobility and reduced egg production. These phenotypes could be significantly modulated by either intervention of dietary iron levels or altering expression of genes involved in iron metabolism. Further study revealed that Pb exposure leads to systemic iron deficiency. Strikingly, reactive oxygen species (ROS) clearance significantly increased iron uptake by restoring the expression of iron metabolism genes in the midgut and subsequently attenuated Pb toxicity. This study highlights the role of ROS in Pb induced iron dyshomeostasis and provides unique insights into understanding the mechanism of Pb toxicity and suggests ideal ways to attenuate Pb toxicity by iron supplementation therapy or ROS clearance.


Assuntos
Anemia Ferropriva/etiologia , Drosophila melanogaster/metabolismo , Chumbo/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Anemia Ferropriva/induzido quimicamente , Animais , Dieta , Drosophila melanogaster/efeitos dos fármacos , Homeostase , Humanos , Ferro/metabolismo , Chumbo/metabolismo , Espécies Reativas de Oxigênio/metabolismo
14.
Cell Rep ; 30(1): 98-111.e5, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31914402

RESUMO

Squamous cell carcinoma (SCC) is an aggressive epithelial malignancy, yet the molecular mechanisms underlying SCC development are elusive. ARID1A is frequently mutated in various cancer types, but both mutation rates and expression levels of ARID1A are ubiquitously low in SCCs. Here, we reveal that excessive protein degradation mediated by the ubiquitin-proteasome system (UPS) contributes to the loss of ARID1A expression in SCC. We identify that the E3 ligase TRIM32 and the deubiquitinase USP11 play key roles in controlling ARID1A stability. TRIM32 depletion inhibits SCC cell proliferation, metastasis, and chemoresistance by stabilizing ARID1A, while USP11 depletion promotes SCC development by promoting ARID1A degradation. We show that syndecan-2 (SDC2) is the downstream target of both ARID1A and USP11 and that SDC2 depletion abolishes the oncogenic function of ARID1A loss. In summary, our data reveal UPS-mediated protein degradation as a mechanism underlying ARID1A loss and propose an important role for the TRIM32/USP11-ARID1A-SDC2 axis in SCC.

15.
J Phys Chem Lett ; 11(1): 243-248, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31724400

RESUMO

The fundamental understanding of electrochemistry urges accurate knowledge of all interfacial properties at the molecular level, but the retrieval of such information is a real challenge. Optical spectroscopies facilitated by surface plasmon enhancement can shed light on this field, yet past studies relied on either highly inhomogeneous "hot spots" or planar plasmon modes with limited enhancement. Here we report an in situ sum-frequency vibrational spectroscopy scheme using plasmonic nanogratings, which enable strong, coherent surface plasmon excitation even on planar electrodes. With two classical reactions, the gold oxidation and pyridine adsorption in water, we demonstrate the realization of coherent vibrational spectroscopy in the strong absorption region, revealing the polar orientation and ordering of interfacial species that are crucial toward the mechanistic understanding of electrochemical phenomena.

16.
Glob Chang Biol ; 26(2): 682-696, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31596019

RESUMO

Arctic and boreal ecosystems play an important role in the global carbon (C) budget, and whether they act as a future net C sink or source depends on climate and environmental change. Here, we used complementary in situ measurements, model simulations, and satellite observations to investigate the net carbon dioxide (CO2 ) seasonal cycle and its climatic and environmental controls across Alaska and northwestern Canada during the anomalously warm winter to spring conditions of 2015 and 2016 (relative to 2010-2014). In the warm spring, we found that photosynthesis was enhanced more than respiration, leading to greater CO2 uptake. However, photosynthetic enhancement from spring warming was partially offset by greater ecosystem respiration during the preceding anomalously warm winter, resulting in nearly neutral effects on the annual net CO2 balance. Eddy covariance CO2 flux measurements showed that air temperature has a primary influence on net CO2 exchange in winter and spring, while soil moisture has a primary control on net CO2 exchange in the fall. The net CO2 exchange was generally more moisture limited in the boreal region than in the Arctic tundra. Our analysis indicates complex seasonal interactions of underlying C cycle processes in response to changing climate and hydrology that may not manifest in changes in net annual CO2 exchange. Therefore, a better understanding of the seasonal response of C cycle processes may provide important insights for predicting future carbon-climate feedbacks and their consequences on atmospheric CO2 dynamics in the northern high latitudes.

17.
Cancer Res Treat ; 52(1): 207-217, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31291712

RESUMO

PURPOSE: A cross-sectional survey was conducted to evaluate the differences on awareness and attitude towards human papillomavirus (HPV) and its vaccine between local and migrant residents who participated in cervical cancer screening in Shenzhen, China. Materials and Methods: A total of 9,855 females sampled from healthcare institutions in 20 street blocks through the Cervical Cancer Prevention Network were surveyed in this study by a self-administered questionnaire. Multivariate logistic regression was conducted to explore the role of the hukou and resident status in the willingness to receive HPV vaccination. RESULTS: Local residents had a relatively higher awareness of HPV (62.0% vs. 35.6% vs. 29.9%, p < 0.001) and its vaccine (35.3% vs. 15.4% vs. 14.8%, p < 0.001), as well as a higher willingness to receive HPV vaccination (68.5% vs. 62.5% vs. 56.2%, p < 0.001) than non-permanent residents and floating population. Except for age, education level, marital status, monthly income, having daughter(s), and heard of HPV and its vaccine, the hukou and resident status significantly associated with the willingness to receive HPV vaccination (local residents vs. floating population: odds ratio, 1.216; 95% confidence interval, 1.057 to 1.398). None significant difference on the associated factors was found between local residents and internal migrants (p for interactions > 0.05). CONCLUSION: Inequalities in awareness and attitude towards HPV and its vaccine existed between local and migrant residents in Shenzhen. The hukou and resident status did impact on the willingness to receive HPV vaccination, therefore, it is critical to implement effective health education campaigns on HPV and its vaccine among internal migrants.

18.
Clin Gastroenterol Hepatol ; 18(3): 756-757, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31394287
19.
Nucleic Acids Res ; 48(D1): D40-D44, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31428785

RESUMO

Epigenetic alterations, including 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and nucleosome positioning (NP), in cell-free DNA (cfDNA) have been widely observed in human diseases, and many available cfDNA-based epigenome-wide profiles exhibit high sensitivity and specificity in disease detection and classification. However, due to the lack of efficient collection, standardized quality control, and analysis procedures, efficiently integrating and reusing these data remain considerable challenges. Here, we introduce CFEA (http://www.bio-data.cn/CFEA), a cell-free epigenome database dedicated to three types of widely adopted epigenetic modifications (5mC, 5hmC and NP) involved in 27 human diseases. We developed bioinformatic pipelines for quality control and standard data processing and an easy-to-use web interface to facilitate the query, visualization and download of these cell-free epigenome data. We also manually curated related biological and clinical information for each profile, allowing users to better browse and compare cfDNA epigenomes at a specific stage (such as early- or metastasis-stage) of cancer development. CFEA provides a comprehensive and timely resource to the scientific community and supports the development of liquid biopsy-based biomarkers for various human diseases.

20.
Microbiol Res ; 231: 126371, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31734584

RESUMO

Eleven soil samples were collected from different plantations at the Forestry Model Base, Northeast Forestry University, China (45°43'10″N, 126°37'15″E), and 122 Trichoderma strains (T1-T122) were isolated. Nine Trichoderma species were identified based on morphological and molecular classification methods. The diversity of woody fungi was analyzed based on the type and quantity of Trichoderma spp. in the soil samples isolated from each plantation. Subdominant T. pseudoharzianum T17 (TpsT17) was screened and its biocontrol potential against Fusarium oxysporum CFCC86068 (Fox68) and growth promotion of Populus davidiana × P. alba var. pyramidalis (PdPap) seedlings were investigated. Compared with PdPap + Fox68 treatment, PdPap + TpsT17 + Fox68 treatment had an obvious antagonistic effect on Fox68 based on the status of roots and stomata of the poplar seedlings. In addition, pretreatment with TpsT17 increased catalase activity 14-fold and decreased hydrogen peroxide and malondialdehyde concentrations 2.57- and 7-fold, respectively, in the PdPap + TpsT17 + Fox68 treatment compared with the PdPap + Fox68 treatment. The transcription levels of PR1, JAZ6751, MYC2, MP, and JAR1 in PdPap + TpsT17+Fox68-treated plants were upregulated 5.75-, 5.63-, 14.88-, 8.24-, and 10.45-fold, respectively, at 3 d, while LAX2 exhibited little change in comparison with the level in PdPap + Fox-treated plants. TpsT17 was detected in the roots and stems of PdPap + TpsT17- and PdPap + TpsT17+Fox68-treated PdPap 28 d after inoculation, which demonstrated the endogenous capacity of TpsT17.

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