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1.
Spinal Cord ; 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31586154

RESUMO

STUDY DESIGN: A multicentre retrospective study. OBJECTIVE: A multicentre retrospective study was performed to observe the changes in serum cystatin C (CysC) levels in patients with acute spinal cord injury (SCI). SETTING: Four hospitals in China. METHODS: Over a 5-year study period, the CysC, creatinine (Cr), and blood urea nitrogen (BUN) levels of people who had incurred SCI in the preceding 7 days were collected and compared with those of people with limb fracture (LF) who were matched for injury time and gender. People with SCI also were grouped by injury duration, ASIA Impairment Scale (AIS) grade and the presence or absence of steroid therapy and compared each day. RESULTS: Three hundred and twenty-three samples from people with SCI were retrospectively collected; their mean serum CysC levels were significantly higher than those of people with LF (p < 0.001); No significant difference was observed in Cr or BUN levels between the two groups (p > 0.14). CysC levels increased on the second day, peaked on day 3, and returned to normal on day 5. The more severely injured individuals had higher CysC levels. Steroid therapy or not had no influence for CysC levels. CONCLUSION: CysC levels are increased in patients with acute SCI, possibly as a direct result of injury. Serum CysC is a potential biomarker of SCI.

2.
Redox Biol ; 28: 101306, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31539804

RESUMO

Interleukin (IL)-1ß and tumor necrosis factor (TNF)-α, in particular, control the degeneration of articular cartilage, making them prime targets for osteoarthritis (OA) therapeutic strategies. Advanced oxidation protein products (AOPPs) are prevalent in numerous diseases. Our previous work demonstrates that intra-articular injections of AOPPs accelerate regression of cartilage in OA models. Whether AOPPs exist in the course of OA and their effects on TNF-α and IL-1ß expression in chondrocytes are still unclear. This study confirmed that AOPPs levels in human synovial fluid were positively associated with severity of OA. We also found AOPPs deposition in articular cartilage in anterior cruciate ligament transection (ACLT) induced rodent OA models. AOPPs increased expression of TNF-α and IL-1ß in chondrocytes in vitro, which was inhibited by pre-treatment with SB202190 (p38-MAPK inhibitor) or apocynin (NADPH oxidase inhibitor) or NOX4 knockdown by siRNAs. Subsequently, we further verified in vivo that exogenous injection of AOPPs in OA mice up-regulated expression of TNF-α and IL-1ß in cartilage, which was blocked by treatment with apocynin. In parallel, apocynin attenuated articular cartilage degeneration resulting in substantially lower OARSI scores. Specifically, apocynin reduced NOX4, p-P38, TNF-α and IL-1ß and increased collagen II and glycosaminoglycan (GAG). This study demonstrated that AOPPs increased expression of TNF-α and IL-1ß in chondrocytes via the NADPH oxidase4-dependent and p38-MAPK mediated pathway, and accelerated cartilage degeneration in OA progression. These findings suggest an endogenous pathogenic role of AOPPs in OA progression. Targeting AOPPs-triggered cellular mechanisms might be a promising therapeutic option for patients with OA.

3.
J Cell Physiol ; 2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31283006

RESUMO

Epigenetic alterations, especially histone modification, play vital roles in the pathogenesis of colon cancer. Upregulation of the enhancer of zeste homolog 2 (EZH2) has been reported to contribute to the initiation and progression of colon cancer. This study analyzed the association between EZH2 and phosphorylation of H2B at tyrosine 37 (H2BY37ph ) in colon cancer tissues and cells, along with the influences of the EZH2-H2BY37ph axis on colon cancer cell autophagy. Immunohistochemistry was utilized to assess EZH2 and H2BY37ph expressions in clinical samples of colon cancer. Cell transfection was carried out to alter EZH2 and H2BY37ph expressions in colon cancer cells. Co-immunoprecipitation analysis and glutathione-S-transferase (GST) pull down assay were conducted to analyze the association between EZH2 and H2BY37ph . Western blotting was utilized to measure proteins expressions related to cell autophagy. We found that there was a positive association between EZH2 and H2BY37ph in colon cancer tissues and cells. EZH2 directly interacted with H2B and promoted H2BY37ph in colon cancer cells using ATP as a phosphate donor. Moreover, EZH2 levated colon cancer cell autophagy in starvation condition. H2BY37ph was required for EZH2-elevated colon cancer cell autophagy under starvation condition. The EZH2-H2BY37ph axis elevated colon cancer cell autophagy possibly via activating transcriptional regulation of ATG genes. In conclusion, EZH2-elevated colon cancer initiation and progression at least in part via inducing colon cancer cell autophagy. EZH2 could phosphorylate H2BY37 and then induce transcription activation of ATG genes in colon cancer cells under starvation condition.

4.
Biomed Res Int ; 2019: 3808432, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31317026

RESUMO

The aberrant expression of Eya2 has been observed in a wide range of cancer types. However, the clinical significance and biological effects of EYA2 in human prostate cancer remain unknown. In this study, we showed that increased levels of Eya2 protein correlated with advanced TNM stage, T stage, and a higher Gleason score. Data from the Cancer Genome Atlas (TCGA) prostate cohort consistently revealed that Eya2 mRNA was positively correlated with a higher Gleason score, higher T stage, and positive nodal metastasis in prostate cancer. Furthermore, data from the Oncomine database showed increased levels of EYA2 mRNA expression in prostate cancer tissues compared with normal tissues. Eya2 protein expression was also higher in prostate cancer cell lines compared with a normal RWPE-1 cell line. We selected LNCaP and PC-3 cell lines for plasmid overexpression and shRNA knockdown. CCK-8, colony formation, and Matrigel invasion assays demonstrated that the overexpression of Eya2 promoted proliferation, colony number, and invasion while Eya2 shRNA inhibited proliferation rate, colony formation, and invasion ability. CCK-8 and Annexin V assays showed that Eya2 reduced sensitivity to docetaxel and docetaxel-induced apoptosis while Eya2 shRNA showed the opposite effects. The overexpression of Eya2 also downregulated the cleavage of caspase3 and PARP while Eya2 depletion upregulated caspase3 and PARP cleavage. Notably, JC-1 staining demonstrated that Eya2 upregulated mitochondrial membrane potential. We further revealed that the overexpression of Eya2 upregulated Bcl-2, matrix metalloproteinase 7 (MMP7), and AKT phosphorylation. Accordingly, data from the TCGA prostate cohort indicated that EYA2 mRNA was positively correlated with the expression of Bcl-2 and MMP7. The inhibition of AKT attenuated EYA2-induced Bcl-2 upregulation. In conclusion, our data demonstrated that Eya2 was upregulated in prostate cancers. EYA2 promotes cell proliferation and invasion as well as cancer progression by regulating docetaxel sensitivity and mitochondrial membrane potential, possibly via the AKT/Bcl-2 axis.

5.
Talanta ; 204: 379-385, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31357309

RESUMO

Fabricating simple, accurate and user-friendly diagnostic device for "point of care testing" (POCT) applications is one of the most challenging objectives in the analytical field. Hemin detection is important for drugs monitoring, diagnosis, and forensic latent bloodstain imaging. Herein is developed, luminol chemiluminescence biosensor for hemin detection using artesunate as coreactant. A possible mechanism to account for the chemiluminescence reaction is discussed. Hemin was detected using both photomultiplier tube (PMT) and smartphone as detector. The detection limit for hemin using smartphone as detector is 20 nM, enabling the visual detection of hemin in blood sample with a dilution factor of blood up to 120,000. While PMT detector is used, the system is able to detect hemin down to 0.22 nM. In addition to high sensitivity, this sensing system exhibit high selectivity. It can successfully distinguish bloodstain from other stains while applying the system for point of care testing using smart phone as detector. Moreover, the system can detect artesunate with a linear range from 0.1 nM to 1.0 µM with a limit of detection of 0.078 nM.

6.
Food Chem ; 295: 423-431, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31174778

RESUMO

Effects of natural phenolics on the shelf life of dried scallop adductor muscle predicted by accelerated shelf life testing (ALST) combined with Arrhenius model were investigated. This allows the food industries to reliably and rapidly determine the shelf life of dried shellfish species treated with antioxidants. The shelf life of dried scallop adductor muscle treated with antioxidants of bamboo leaves (AOB) and tea polyphenols (TP) was more than 1.70-fold that of dried control scallop adductor muscle. Thus, the highly nutritional value of dried scallop adductor muscle, based on its lipid constituents, is maintained during storage. OXITEST method further confirmed the improvement of lipid stability of antioxidant treated dried scallop adductor muscle by protecting polyunsaturated fatty acids, especially eicosapentaenoic and docosahexaenoic acids, against autoxidation. Moreover, the natural phenolics employed effectively limited lipid oxidation by breaking the autoxidative chain reaction and/or inhibiting free radical formation in dried scallop adductor muscle during storage.


Assuntos
Armazenamento de Alimentos , Lipídeos/química , Pectinidae/química , Polifenóis/química , Frutos do Mar , Animais , Antioxidantes/química , Camellia sinensis/química , Ácidos Graxos Insaturados/análise , Ácidos Graxos Insaturados/química , Liofilização , Músculo Esquelético/química , Valor Nutritivo , Oxirredução , Folhas de Planta/química , Carbamilação de Proteínas , Sasa/química
7.
ACS Nano ; 13(7): 8004-8011, 2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31241301

RESUMO

Phase transformation in emerging two-dimensional (2D) materials is crucial for understanding and controlling the interplay between structure and electronic properties. In this work, we investigate 2D In2Se3 synthesized via chemical vapor deposition, a recently discovered 2D ferroelectric material. We observed that In2Se3 layers with thickness ranging from a single layer to ∼20 layers stabilized at the ß phase with a superstructure at room temperature. At around 180 K, the ß phase converted to a more stable ß' phase that was distinct from previously reported phases in 2D In2Se3. The kinetics of the reversible thermally driven ß-to-ß' phase transformation was investigated by temperature-dependent transmission electron microscopy and Raman spectroscopy, corroborated with the expected minimum-energy pathways obtained from our first-principles calculations. Furthermore, density functional theory calculations reveal in-plane ferroelectricity in the ß' phase. Scanning tunneling spectroscopy measurements show that the indirect bandgap of monolayer ß' In2Se3 is 2.50 eV, which is larger than that of the multilayer form with a measured value of 2.05 eV. Our results on the reversible thermally driven phase transformation in 2D In2Se3 with thickness down to the monolayer limit and the associated electronic properties will provide insights to tune the functionalities of 2D In2Se3 and other emerging 2D ferroelectric materials and shed light on their numerous potential applications (e.g., nonvolatile memory devices).

8.
Food Chem ; 297: 124951, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31253340

RESUMO

The shelf lives of hot-air-dried (HD) and freeze-dried (FD) Penaeus vannamei were predicted by accelerated storage testing combined with Arrhenius Equation. Meanwhile, the changes in lipid profiles and colour of the dried shrimps during storage were investigated. The predicted shelf life of FD shrimp was more than 1.47-fold than that of HD shrimp. Compared to HD shrimp, FD shrimp had lower levels of oxidation parameters such as peroxide value (POV) and thiobarbituric acid-reactive substances value (TBARS) during storage. In addition, FD shrimp had less reduction in lipid components such as triacylglycerol (TAG), phosphatidylcholine (PC), phosphatidylethanolamine (PE) and all fatty acids including saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) after storage. Moreover, FD shrimp showed a less significant decrease in redness value and increase in yellowness value. Therefore, FD shrimps have better quality stability during storage and then possess longer shelf life than HD shrimp.


Assuntos
Armazenamento de Alimentos/métodos , Lipídeos/química , Penaeidae/química , Frutos do Mar/análise , Animais , Cor , Ácidos Graxos/análise , Ácidos Graxos/química , Manipulação de Alimentos/métodos , Radicais Livres/análise , Radicais Livres/química , Liofilização , Lipídeos/análise , Valor Nutritivo , Oxirredução , Fosfatidilcolinas/análise , Fosfatidilcolinas/química , Triglicerídeos/análise , Triglicerídeos/química , Xantofilas/análise
9.
ACS Appl Mater Interfaces ; 11(23): 20979-20986, 2019 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-31119937

RESUMO

Alloying is an effective way to modulate material's properties. In particular, graded alloying within a single domain of two-dimensional transition-metal chalcogenide (2D-TMC) is of great technological importance, for example, for achieving band gap modulations. Here, we report a facile method to grow gradient alloying of Mo1- xW xS2 monolayers with large domain sizes and high crystal qualities via the chemical vapor deposition technique. The as-grown Mo1- xW xS2 monolayers have a gradient composition of W from x = ∼0 to ∼1 in a single domain with a lateral dimension up to 300 µm, and the span in band gap can be readily tuned. Owing to the grading in band offsets, the compositionally graded Mo1- xW xS2 alloy monolayer demonstrates an excellent rectifying effect with the ratio of forward to reverse current up to ∼104. Moreover, phototransistors based on the compositionally graded Mo1- xW xS2 monolayers exhibit a high responsivity up to 298.4 A/W in the visible light regime, and particularly a decent responsivity of 28.7 A/W in the near-infrared regime. The control of band gap offset gradient and span in alloyed 2D-TMC semiconductors provides an additional degree of freedom in designing fascinating applications in achieving multifunctional optoelectronic devices on individual substrates.

10.
Lipids ; 54(5): 347-356, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-31087412

RESUMO

Total lipids were extracted from razor clams Sinonovacula constricta and Solen gouldi, and the molecular species of glycerophospholipid (Gpl) including choline glycerophospholipid (ChoGpl), ethanolamine glycerophospholipid (EtnGpl), serine glycerophospholipid (SerGpl), inositol glycerophospholipid (InsGpl), lysoChoGpl, lysoEtnGpl, and lysoSerGpl were characterized using a direct-infusion tandem mass spectrometric method for the first time. Meanwhile, the lipid class composition and phospholipid (PL) class composition as well as the fatty acid (FA) composition of total lipids, triacylglycerol (TAG), and PL were also investigated. About 238 and 235 molecular species were characterized, respectively, in Sinonovacula constricta and Solen gouldi. The majority of the dominant Gpl molecular species contained n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA), especially docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). Also, razor clam lipids contained a high-proportioned PL (52.19-65.41% of total lipids) and PUFA (47.94-54.81 mol%). Furthermore, PL contained a higher proportion of PUFA (63.05-67.13 mol%), especially DHA (20.04-22.47 mol%) and EPA (16.27-21.46 mol%) than TAG (the corresponding values being 33.73-34.45, 11.95-12.27, and 8.13-0.8.99 mol%, respectively). Meanwhile, phosphatidylcholine (44.38-46.21 mol%) and phosphatidylethanolamine (38.84-39.95 mol%) were dominant among PL. In consideration of the high proportion of PUFA-enriched Gpl, razor clam plays a great role in promoting human health.

11.
Nanotechnology ; 30(34): 345203, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31108474

RESUMO

In two-dimensional layered materials, layer number and stacking order have strong effects on the optical and electronic properties. Tungsten disulfide (WS2) crystal, as one important member among transition metal dichalcogenides, has been usually prepared in a layered 2H prototype structure with space group P63/mmc ([Formula: see text]) in spite of many other expected ones such as 3R. Here, we report simultaneous growth of 2H and 3R stacked multilayer (ML) WS2 crystals in large scale by chemical vapor deposition and effects of layer number and stacking order on optical and electronic properties. As revealed in Raman and photoluminescence (PL) measurements, with an increase in layer number, 2H and 3R stacked ML WS2 crystals show similar variation of PL and Raman peaks in position and intensity. Compared to 2H stacked ML WS2, however, 3R stacked one always exhibits the larger red (blue) shift of Raman [Formula: see text] (A1g) peak and the appearance of PL A, B and I peaks at lower energies. Thereby, PL and Raman features depend on not only layer number but also stacking order. In addition, circularly polarized luminescence from two prototype WS2 crystals under circularly polarized excitation has also been investigated, showing obvious spin or valley polarization of these CVD-grown multilayer WS2 crystals.

12.
J Neurosurg Spine ; : 1-5, 2019 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-30797204

RESUMO

OBJECTIVEThe aim of this study was to evaluate the effect of lumbar sacralization on the level of vertebral slip and disc degeneration in patients with L4 spondylolysis.METHODSThe authors analyzed data from 102 cases in which patients underwent surgical treatment for L4 spondylolysis and spondylolisthesis at their institution between March 2007 and September 2016. Lumbar sacralization was characterized by the presence of pseudarthrosis and/or bony fusion between the L5 transverse process and sacrum, and the type of lumbosacral transitional vertebra (LSTV) was evaluated with the Castellvi classification. The amount of vertebral slippage was measured using the Taillard technique and Meyerding grade. Degeneration of the L4-5 segment was quantified using the Pfirrmann and Modic classifications. Patients were divided into 2 groups based on the presence or absence of sacralization, and the amount of vertebral slip and degeneration of the L4-5 segment was compared between groups.RESULTSLumbar sacralization was present in 37 (36%) of 102 patients with L4 spondylolysis. The LSTV was type IIa in 10 cases, type IIb in 7, type IIIa in 2, and type IIIb in 18. The levels of vertebral slip and disc degeneration in the group of patients with sacralization were significantly greater than in the group without sacralization. No significant difference was found between the 2 groups with respect to Modic changes.CONCLUSIONSThe increased stability between a sacralized L5 and the sacrum may predispose the L4-5 segment to greater instability and disc degeneration in patients with L4 spondylolysis.

13.
Gene ; 697: 144-151, 2019 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-30802542

RESUMO

ß-1,3-Glucan recognition protein (ßGRP) is an important pattern recognition protein, which could trigger immune response to eliminate pathogens by identifying and combining the pathogenic bacteria. In the present study, a ß-1,3-glucan recognition protein gene (ApßGRP) was cloned from a desert beetle Anatolica polita based on the EST sequence of ApßGRP in the suppression subtractive cDNA library. Quantitative real-time PCR (qRT-PCR) results showed that ApßGRP transcript in A. polita was significantly upregulated under the challenge of Escherichia coli and Staphylococcus aureus. Western blot analysis indicated that recombinant ApßGRP expressed in E. coli BL21, has the ability of binding to E. coli and S. aureus. Moreover, agglutination assay suggested that recombinant ApßGRP could agglutinate E. coli, S. aureus and Saccharomyces cerevisiae. The predicted 3D structure showed that ApßGRP possesses a typical ß-glucan recognition domain with seven ß-strands structures and conserved amino acid sequence. These data indicate that ApßGRP may be involved in immune defense in A. polita and could recognize and bind the bacteria against the invasion of external pathogens.


Assuntos
Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Besouros/genética , Lectinas/genética , Lectinas/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Escherichia coli/patogenicidade , Glucanos , Proteínas de Insetos/genética , Ligação Proteica , Staphylococcus aureus/patogenicidade , Regulação para Cima , beta-Glucanas/imunologia , beta-Glucanas/metabolismo
14.
Food Chem ; 281: 251-260, 2019 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-30658755

RESUMO

Lipid hydrolysis and oxidation occurred in Argopecten irradians adductor muscle during hot air drying. Using an in vivo imaging system, we found that antioxidants of bamboo leaves (AOB) could diffuse into the adductor muscle upon marinating. Both tea polyphenols (TP) and AOB efficiently retarded lipid oxidation but had a slight effect on lipid hydrolysis during drying process. The in situ antioxidant mechanisms of AOB as well as TP were revealed, including quenching of free radicals detected by electron spin resonance, chelating metal ions determined by confocal laser scanning microscopy and inhibiting lipoxygenase. Less than 8% of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in AOB and TP marinated adductor muscle were decreased compared to more than 28% decrease in control adductor muscle during the drying process. Overall, these natural antioxidants, TP and AOB, efficiently maintained high nutritive value of adductor muscle, especially, their lipid quality.


Assuntos
Antioxidantes/análise , Dessecação , Manipulação de Alimentos , Pectinidae , Polifenóis/análise , Alimentos Marinhos/análise , Chá/química , Animais , Ácidos Docosa-Hexaenoicos/análise , Ácido Eicosapentaenoico/análise , Ácidos Graxos Insaturados/análise , Metabolismo dos Lipídeos , Valor Nutritivo , Fosforilcolina/análise , Extratos Vegetais/análise , Folhas de Planta/química , Sasa/química , Substâncias Reativas com Ácido Tiobarbitúrico/análise
15.
Gene ; 690: 21-29, 2019 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-30593914

RESUMO

Peptidoglycan recognition proteins (PGRPs) widely distributed in invertebrates and vertebrates are pattern-recognition molecules in innate immunity. In the present study, a novel short PGRP gene, designated as ApPGRP was identified from Anatolica polita. The deduced amino acid sequence of ApPGRP is composed of 196 residues and contains a conserved PGRP domain at the C-terminus. The phylogenetic tree showed that ApPGRP shared high homology of amino acid sequence with TcPGRP2 from Tribolium castaneum. The recombinant protein of ApPGRP exhibited binding activity toward Escherichia coli and DAP-type PGN from E. coli. Quantitative real time PCR (RT-qPCR) analysis indicated that the relative expression level of ApPGRP was up-regulated significantly after E. coli and DAP-type PGN challenge in the fifth instar larvae of A. polita. Moreover, the expressions of three antimicrobial peptides (AMPs) (ApAttacin 1, ApAttacin 2 and ApColeoptericin) were significantly increased after E. coli and DAP-type PGN challenge. RNA interference (RNAi) experiments showed that the expressions of three AMPs in the larvae of A. polita were significantly decreased after injection of ApPGRP dsRNA. Furthermore, the expressions of three AMPs in the larvae injected were still significantly decreased after E. coli challenge compared to the control samples without dsRNA injection. The predicted 3D structure showed that the ApPGRP could form the protein core of five ß-sheet and three ɑ-helices, which would be involved in specific PGN recognition. These results suggested that ApPGRP may play an important role in the immune response to E. coli infection and function as a receptor for antimicrobial peptide gene induction in Anatolica polita.


Assuntos
Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Escherichia coli/patogenicidade , Tenebrio/microbiologia , Regulação para Cima , Animais , Sequência de Bases , Proteínas de Transporte/química , Clonagem Molecular , Escherichia coli/imunologia , Evolução Molecular , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Modelos Moleculares , Filogenia , Estrutura Secundária de Proteína , Tenebrio/genética , Tenebrio/metabolismo
16.
Food Chem ; 272: 109-116, 2019 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-30309519

RESUMO

Change in quality of mussels (Mytilus edulis) meat when stored at 4 °C was evaluated by determining volatile basic nitrogen (TVB-N), peroxide value (POV), thiobarbituric acid reactive substance (TBARS), total oxidation (TOTOX), lipid class, fatty acid (FA), phosphatidylcholine (PC) and phosphatidylcholine (PE) content, and glycerophospholipid (GP) molecular species. After 4 days of storage, the percentages of triacylglycerol and polar lipid as well as the contents of PC, PE and major GP molecular species significantly decreased (P < 0.05), while the percentages of free FA and monoacylglycerol significantly increased (P < 0.05), indicating the hydrolysis of lipids. The increase in activities of phospholipase and lipase during storage suggests that they may contribute to the hydrolysis of lipids. The cold storage also resulted in significantly increased POV, TBARS and TOTOX as well as lipoxygenase activity (P < 0.05) but slightly decreased percentage of polyunsaturated FA (PUFA), indicating the occurrence of lipid oxidation.


Assuntos
Temperatura Baixa , Armazenamento de Alimentos , Lipídeos/química , Mytilus edulis/química , Animais , Ácidos Graxos/química , Glicerofosfolipídeos/química , Hidrólise , Lipase/química , Oxirredução , Fosfatidilcolinas/química , Alimentos Marinhos/análise , Substâncias Reativas com Ácido Tiobarbitúrico/química
17.
Small ; : e1803513, 2018 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-30427576

RESUMO

Identifying the relationship between catalytic performance and material structure is crucial to establish the design principle for highly active catalysts. Deficiency in BO bond covalency induced by lattice distortion severely restricts the oxygen reduction reaction (ORR) performance for ABO3 -type perovskite oxides. Herein, a rearrangement of hybridization mode for BO bond is used to tune the overlap of the electron cloud between B 3d and O 2p through A-stie doping with larger radius ions. The BO bond covalency is strengthened with a BOB bond angle recovered from intrinsic structural distortion. As a result, the adsorption and the reduction process for O2 on the oxide surface can be promoted via shifting the O-2p band center toward the Fermi Level. Simultaneously, the spin electrons in the Mn 3d orbit become more parallel. It will lead to a high electrical conductivity by the enhanced double exchange process and thereof mitigate the ORR efficiency loss. Further density functional theory calculation reveals that a flat [BO2 ] plane will make contribution to the charge transfer process from lattice oxygen to adsorbed oxygen (mediated with B ions). Through such exploration of the effect of crystal structure on the electronic state of perovskite oxides, a novel insight into design of highly active ORR catalysts is offered.

18.
J Food Sci ; 2018 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-30380147

RESUMO

Clam Ruditapes philippinarum was processed by hot-air drying and the changes of its lipids were evaluated by analyzing lipid classes, phospholipid classes, fatty acids, as well as oxidation parameters including peroxide value (POV), thiobarbituric acid-reactive substances (TBARS) value, total oxidation value (TOTOX), and oxidation test (OXITEST). The hot-air drying process reduced the contents of triacylglycerol, phosphatidylcholine, and phosphatidylethanolamine, indicating the hydrolysis of lipids. Meanwhile, the hot-air drying process significantly decreased the proportion of n-6 polyunsaturated fatty acid (PUFA), and consequently increased the PUFA ratio of n-3/n-6. Interestingly, the POV, TBARS and TOTOX decreased after the hot-air drying process. However, significant decline of the induction period for the dried clam tissue at elevated temperatures indicated their higher oxidation level, poor oxidative stability and reduction of shelf-life. Therefore, OXITEST method turned out to be an effective tool for estimating the level of lipid oxidation for hot-air dried clam.

19.
Cell Death Dis ; 9(10): 1042, 2018 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-30310053

RESUMO

Bladder cancer (BC) is one of the most common tumors in the urinary system. Noncoding RNAs are considered to take part in cellular phenotypes and are emerging as diagnostic and prognostic biomarkers of BC. The aim of this study is to investigate the clinical significance of neuroblastoma- associated transcript 1 (NBAT1) gene and its effects on malignant cellular phenotypes in BC. NBAT1 gene was low-expressed in BC tissues and cell lines and its low-expression was related with high pathological grade and metastasis of BC. Upregulation of NBAT1 gene depressed cell viability and invasiveness of KK47 and T24 cells and arrested KK47 and T24 cells at G1 stage. In addition, NBAT1 could target silence the expression of miR-21-5p in RNA-induced silencing complex-dependent manner. KK47 and T24 cells with miR-21-5p knockdown showed reduced cell viability, G1-stage arrest, and depressed invasiveness. MiR-21-5p mediates the regulatory effects of NBAT1 on malignant cellular phenotypes of BC cells. Moreover, SOCS6 gene was a target gene of miR-21-5p, and miR-21-5p modulated malignant cellular phenotypes of KK47 and T24 cells through targeted silencing of SOCS6. In conclusion, low-expression of NBAT1 is associated with the progress and metastasis of BC, and NBAT1 inhibits malignant cellular phenotypes through miR-21-5p/SOCS6 axis in BC. Our findings help to elucidate the tumorigenesis of BC, and future study will provide a novel therapeutic target for BC.

20.
Front Plant Sci ; 9: 1303, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30283465

RESUMO

The MYB transcription factors (TFs) is a plant TF families, which involves in hormone signal transduction, and abiotic stress tolerance, etc. However, there are few studies on the MYB TFs family and its regulatory mechanism in Tamarix hispida. In this study, 14 MYB genes (named ThMYB1 - ThMYB14) were cloned and characterized from T. hispida. The transcription profiles of ThMYBs in T. hispida under different abiotic stress conditions were monitored using qRT-PCR. Most of studied ThMYBs were significantly downregulated and/or upregulated by salt and osmotic stress, ABA, GA3 and JA treatments in at least one organ. Especially, ThMYB13 was induced in the leaves and roots of T. hispida when exposed to NaCl treatment at all study periods, indicating that it may involve in salt stress. To further study ThMYB13 function, ThMYB13 overexpression and knock-down plants and control plants transformed with an empty pROKII were obtained using a transient transformation system. Overexpression of ThMYB13 in T. hispida displayed the lowest O2-, H2O2 and MDA accumulation, minimal cell death, the most stable K+/Na+ ratio and the lowest electrolyte leakage rate among the three kinds of transient expression in T. hispida. Conversely, the RNAi-silencing, transiently transformed plants displayed the opposite physiological changes. Therefore, ThMYB13 might play a role in salt stress tolerance in transgenic T. hispida plants.

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