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1.
Sci Rep ; 10(1): 3994, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32132599

RESUMO

Severe neurological complications following infective endocarditis remain a major problem with high mortality rate. The long-term neurological consequences following infective endocarditis remain uncertain. Otherwise, neurosurgeries could be performed after these complications; however, few clinical series have reported the results. Therefore, we utilized a large, nationwide database to unveil the long-term mortality and neurosurgical outcome following infective endocarditis. We included patients with a first-time discharge diagnosis of infective endocarditis between January 2001 and December 2013 during hospitalization. Patients were further divided into subgroups consisting of neurological complications under neurosurgical treatment and complications under non-neurosurgical treatment. Long-term result of symptomatic neurological complications after infective endocarditis and all-cause mortality after different kinds of neurosurgeries were analyzed. There were 16,495 patients with infective endocarditis included in this study. Symptomatic neurological complications occurred in 1,035 (6.27%) patients, of which 279 (26.96%) accepted neurosurgical procedures. Annual incidence of neurological complications gradually increased from 3.6% to 7.4% (P < 0.001). The mortality rate among these patients was higher than that among patients without complications (48.5% vs. 46.1%, P = 0.012, increased from 20% initially to nearly 50% over the 5-year follow-up). However, neurosurgery had no effect on the long-term mortality rate (50.9% vs. 47.6%, P = 0.451). Incidence of neurological complications post-infective endocarditis is increasing, and patients with these complications have higher mortality rates than patients without. Neurosurgery in these populations was not associated with higher long-term mortality. Therefore, it should not be ruled out as an option for those with neurological complications.

2.
Cancer Commun (Lond) ; 40(1): 3-15, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32125093

RESUMO

BACKGROUND: Indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan (Trp) catabolism have been demonstrated to play an important role in tumor immunosuppression. This study examined the expression and catalytic activity of IDO1 in penile squamous cell carcinoma (PSCC) and explored their clinical significance. METHODS: IDO1 expression level, serum concentrations of Trp and kynurenine (Kyn) were examined in 114 PSCC patients by immunohistonchemistry and solid-phase extraction-liquid chromatography-tandem mass spectrometry. The survival was analyzed using Kaplan-Meier method and the log-rank test. Hazard ratio of death was analyzed via univariate and multivariate Cox regression. Immune cell types were defined by principal component analysis. The correlativity was assessed by Pearson's correlation analysis. RESULTS: The expression level of IDO1 in PSCC cells was positively correlated with serum Kyn concentration and Kyn/Trp radio (KTR; both P < 0.001) but negatively correlated with serum Trp concentration (P = 0.001). Additionally, IDO1 up-regulation in cancer cells and the increase of serum KTR were significantly associated with advanced N stage (both P < 0.001) and high pathologic grade (P = 0.008 and 0.032, respectively). High expression level of IDO1 in cancer cells and serum KTR were associated with short disease-specific survival (both P < 0.001). However, besides N stage (hazard radio [HR], 6.926; 95% confidence interval [CI], 2.458-19.068; P < 0.001) and pathologic grade (HR, 2.194; 95% CI, 1.021-4.529; P = 0.038), only serum KTR (HR, 2.780; 95% CI, 1.066-7.215; P = 0.036) was an independent predictor for PSCC prognosis. IDO1 expression was positively correlated with the expression of interferon-γ (IFNγ, P < 0.001) and immunosuppressive markers (programmed cell death protein 1, cytotoxic T-lymphocyte-associated protein 4 and programmed death-ligand 1 and 2; all P < 0.05), and the infiltration of immune cells (including cytotoxic T lymphocytes, regulatory T lymphocytes, tumor-associated macrophages, and myeloid-derived suppressor cells; all P < 0.001) in PSCC tissues. Furthermore, the expression of IDO1 was induced by IFNγ in a dose-dependent manner in PSCC cells. CONCLUSIONS: IFNγ-induced IDO1 plays a crucial role in immunoediting and immunosuppression in PSCC. Additionally, serum KTR, an indicator of IDO1 catabolic activity, can be utilized as an independent prognostic factor for PSCC.

3.
Front Neurol ; 11: 168, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32218766

RESUMO

Background: From 2017, the Stroke Health Manager Training Project was carried out by the Chinese Government to strengthen health management and follow-up intervention after ischemic stroke. The aim of this study was to investigate whether after the intervention of the stroke health manager, the control of blood pressure, low-density lipoprotein cholesterol (LDL-C), glucose level, and the use of secondary prevention medications improved 3 months after discharge from our center following ischemic stroke. Methods: The study used a history-controlled approach. Patients who received stroke health manager intervention from May 1, 2018, to March 31, 2019, were considered as the intervention group; those from May 1, 2017, to April 30, 2018, were enrolled as the control group. Stroke health manager intervention included health education, discharge advice, online WeChat public group follow-up, and clinical consultation. Results: In total, 642 patients with ischemic stroke were enrolled in this study (277 in the control group, 365 in the intervention group). At 3 months, the blood pressure, LDL-C and glucose control in the intervention group were better than in the control group (all P < 0.05). At the same time, the overall persistence for secondary prevention medications at 3 months after discharge increased from 201/277 (72.56%) to 303/365 (83.01%, P = 0.001). The persistence for patients taking antiplatelet, hypoglycemic and statins were significantly higher in the intervention group (P < 0.05). Conclusions: Stroke health manager intervention improved the control of blood pressure, LDL-C, glucose levels and the persistence for secondary prevention medications 3 months after discharge.

4.
Bioresour Technol ; 306: 123097, 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-32192958

RESUMO

Solids loading is a key factor in aqueous or gaseous ammonia production from urea. Methane production from urea-pretreated corn stover, as well as the physicochemical characteristics and delignification kinetics of the corn stover, were investigated with four solids loading values (10%-70%) and five ratios of urea to corn stover (1:100-7:10) at 35 °C for 6 weeks. A 1:20 ratio of urea to corn stover was optimal for achieving high lignin removal with ≤50% solids loading, and 7:10 was optimal with 70% solids loading. Under the two optimal conditions, 85.56% and 82.35% of cellulose and 85.76% and 85.49% of hemicellulose were retained. The maximum lignin removal rates of 69.67% and 68.27% and methane production of 294.70 and 292.56 L/kg volatile solids (VS) were achieved, respectively. The delignification kinetics of the urea-pretreated corn stover conformed to three first-order reactions. Most of the lignin was degraded within the first 3 weeks.

5.
J Healthc Eng ; 2020: 3264801, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32184978

RESUMO

Recently, deep reinforcement learning, associated with medical big data generated and collected from medical Internet of Things, is prospective for computer-aided diagnosis and therapy. In this paper, we focus on the application value of the second-generation sequencing technology in the diagnosis and treatment of pulmonary infectious diseases with the aid of the deep reinforcement learning. Specifically, the rapid, comprehensive, and accurate identification of pathogens is a prerequisite for clinicians to choose timely and targeted treatment. Thus, in this work, we present representative deep reinforcement learning methods that are potential to identify pathogens for lung infection treatment. After that, current status of pathogenic diagnosis of pulmonary infectious diseases and their main characteristics are summarized. Furthermore, we analyze the common types of second-generation sequencing technology, which can be used to diagnose lung infection as well. Finally, we point out the challenges and possible future research directions in integrating deep reinforcement learning with second-generation sequencing technology to diagnose and treat lung infection, which is prospective to accelerate the evolution of smart healthcare with medical Internet of Things and big data.

6.
J Immunol ; 204(7): 1736-1745, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32111731

RESUMO

IL-37 is a newly identified immune-suppressive factor; however, the function, cellular sources, and mechanism of IL-37 in humoral immunity and Myasthenia gravis (MG) are still unclear. In this study, we found IL-37 were substantially downregulated in the serum and PBMCs of MG patients compared with healthy controls. The lower IL-37 was associated with severer disease (quantitative MG score) and higher follicular Th (Tfh)/Tfh17 and B cell numbers. Flow cytometry analysis revealed that IL-37 was mainly produced by CD4+ T cells without overlapping with Th1, Th17, and Tfh subsets in MG patients. Regulatory IL-37+ T cell rarely expressed Foxp3 and CD25 but produced numerous IL-4. Tfh and B cell expressed high levels of SIGIRR, the receptor of IL-37, in MG patients. Mechanically, IL-37 directly bond to SIGIRR, repressed the proliferation, cytokine production of Tfh and B cells, and the secretion of autoantibody via inhibition of STAT3 signaling in Tfh and B cells.

7.
Andrology ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32012485

RESUMO

BACKGROUND: Diabetes mellitus (DM) induces impairment of male reproductive system and is considered as a key factor that could partially provide an explanation for male infertility. Thus, understanding the mechanism underlying DM-induced infertility will aid in the identification of novel therapeutic stratagems. OBJECTIVES: To delineate the role of ROS/JAK2/NFκB pathway in DM-induced low reproductive function and impaired spermatogenesis. Additionally, to investigate the protective effect of monomeric Berberine (BB) that inhibits ROS/JAK2/NFκB pathway, in the pathogenesis of DM-induced infertility. METHODS: 12-week-old male Sprague-Dawley rats were divided into four groups: control group, DM group, control plus BB group, and DM plus BB group. Streptozotocin was used to induce DM. After treating the rats with BB for 4 weeks, fertility tests were conducted to investigate the reproductive function, and testis weight along with sperm motility was assessed through microscope. Oxidative stress was evaluated by DHE staining. TUNEL staining was utilized to detect the state of apoptosis. Cell experiments were carried out to define the role of BB in vitro. Immunohistochemistry, immunofluorescence, and Western blotting were employed to measure the protein expression. RESULTS: Our results indicate that the reproductive function of DM rats was low, accompanied by decreased testis weight and sperm motility in addition to the impairment of the seminiferous tubules. However, there was a significant improvement in the reproductive function parameters in the BB-treated DM rats. Subsequently, our data revealed that DM rats produce an increased level of ROS in the testis, which activates JAK2 further activating the NFκB pathway, leading to increased apoptosis and impaired cells in the testicles. However, BB could attenuate the ROS production and abrogate activation of JAK2/NFκB pathway, thus inhibiting the apoptosis in the testicular cells of DM rats. CONCLUSION: ROS/JAK2/NFκB pathway is involved in the DM-induced low reproductive function and impaired spermatogenesis. BB can play a protective role in preserving the reproductive function and spermatogenesis in DM by inhibiting ROS/JAK2/NFκB pathway.

8.
Int J Infect Dis ; 93: 182-191, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32061862

RESUMO

BACKGROUND: The clinical utilisation of deep sequencing in HIV treatment has been hindered due to its unknown correlation with standard Sanger genotyping and the undetermined value of minority drug resistance mutation (DRM) detection. OBJECTIVES: To compare deep sequencing performance to standard Sanger genotyping with clinical samples, in an effort to delineate the correlation between the results from the two methods and to find the optimal deep sequencing threshold for clinical utilisation. METHODS: We conducted a retrospective study using stored plasma collected from August 2014 to March 2018 for HIV genotyping with the commercial Sanger genotyping kit. Samples with available Sanger genotyping reports were further deep sequenced. Drug resistance was interpreted according to the Stanford HIV drug resistance database algorithm. RESULTS: At 15-25% minority detection thresholds, 9-15% cases had underestimated DRMs by Sanger sequencing. The concordance between the Sanger and deep sequencing reports was 68-82% in protease-reverse transcriptase region and 88-97% in integrase region at 5-25% thresholds. The undetected drug resistant minority variants by Sanger sequencing contributed to the lower negative predictive value of Sanger genotyping in cases harbouring DRMs. CONCLUSIONS: Use of deep sequencing improved detection of antiretroviral resistance mutations especially in cases with virological failure or previous treatment interruption. Deep sequencing with 10-15% detection thresholds may be considered a suitable substitute for Sanger sequencing on antiretroviral DRM detection.

9.
J Mater Chem B ; 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31984984

RESUMO

Motion recognition and information interaction sensors with flexibility and stretchability are key functional modules as interactive media between the mechanical motions and electric signals in an intelligent robotic and rehabilitation training system. Nanogenerators have many useful applications in the field of intelligent interaction, with the advantages of a self-powered sensing ability, easy fabrication, considerable sensitivity and reliability. However, the singularity of the sensing mode limits its applications. Hence, in this research, a flexible and stretchable dual mode nanogenerator (FSDM-NG) for human motion sensing and information interaction, based on the integration of piezoelectric and triboelectric principles was developed. In piezoelectric mode, the FSDM-NG can effectively monitor the bending angle of joints (finger, wrist and elbow) from 30° to 90°. In triboelectric mode, text and logic information transfer are encoded using Morse code and logic gates, respectively. In addition, the device has good adhesion and biosafety, and is robust which makes it work normally even in under water environments. Combining these two sensing mechanisms, multiple modes of sensing from touch and stretch based on the FSDM-NG can be achieved for information interaction in real time. The proposed sensor has the potential to be adapted for more complex sensing, which may provide new applications for intelligent interaction of robots and in the rehabilitation training field.

10.
BMC Cancer ; 20(1): 61, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31992244

RESUMO

BACKGROUND: To explore the influencing factors of perioperative renal function change and their relationship with prognosis on renal cell carcinoma (RCC) patients with tumor thrombus after nephrectomy and thrombectomy. METHODS: The clinical and pathological data of 135 patients with RCC and tumor thrombus, who underwent nephrectomy and thrombectomy at Peking University Third Hospital from May 2015 to July 2018, was retrospectively analyzed. Absolute change in estimated glomerular filtration rate (eGFR) (ACE) and percent change in eGFR (PCE) were calculated by preoperative and postoperative renal function. Linear regression analysis was used to explore the influencing factors of ACE and PCE, and logistic regression analysis was used to explore the influencing factors of worse postoperative renal function [eGFR≤60 mL/(min × 1.73 m^2)]. Cancer-specific survival (CSS) was estimated by Kaplan-Meier method and multivariate Cox regression, which were used to explore the effect of ACE and PCE on prognosis. RESULTS: Of all the 135 patients, 101 patients (74.8%) were male and 34 patients (25.2%) were female. The mean preoperative eGFR was 73.9 ± 21.8 mL/(min × 1.73 m^2) and postoperative eGFR was 69.5 ± 25.2 mL/(min × 1.73 m^2). In multivariate linear regression analysis, preoperative eGFR (P < 0.001) and pathological type (P = 0.038) were significant predictive factors of ACE. In aspect of PCE, preoperative eGFR (P < 0.001) and pathological type (P = 0.002) were significant predictors. In multivariate logistic regression analysis, preoperative eGFR (P = 0.016) was the only risk factor of predicting worse postoperative renal function. During follow-up, 22 patients (16.3%) were dead due to RCC. According to ROC analysis, the cut off value of ACE and PCE was 13.9 and 0.16, respectively. ACE> 13.9 and PCE > 0.16 indicated worse CSS (P = 0.006 and P = 0.047, respectively). However, in multivariate Cox regression analysis of several related factors, perinephric tissues invasion (P = 0.001), sarcomatoid differentiation (P = 0.001) and ACE> 13.9 (P = 0.002) were significant prognostic factors for CSS. PCE > 0.16 seemed to be not (P = 0.055). CONCLUSION: We explored several clinicopathological risk factors of predicting renal function change and their relationship with prognosis of RCC patients with tumor thrombus after nephrectomy and thrombectomy. The renal function change, which was associated with preoperative eGFR and pathological type, was prognostic risk factor for CSS and ACE> 13.9 indicated the worse prognosis.

11.
Water Res ; 168: 115154, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31630020

RESUMO

Pyrrhotite is often considered as a gangue mineral, and discarded in mine wastes and tailings. Glyphosate and fertilizer, often excessively used in agriculture, flow into water bodies with agriculture runoff, and cause pollution of water bodies. In this study, the pyrrhotite was used as a substrate in a pilot constructed wetland (CW) to remove the glyphosate and nutrients from simulated agriculture runoff. In nearly one year, the pilot pyrrhotite constructed wetland (Pyrr-CW) removed 90.3 ±â€¯6.1% of glyphosate, 88.2 ±â€¯5.1 of total phosphorus (TP) and 60.40 ±â€¯5.60% of total nitrogen (TN) on average, much higher than the control CW. The abundances of sulfur-oxidizing bacteria, such as Sulfurifustis, Sulfuriferula and Thiobacillus, were much higher in the Pyrr-CW than those in the control CW. In the Pyrr-CW goethite was produced by pyrrhotite aerobic oxidation (PAO) and pyrrhotite autotrophic denitrification (PAD) continuously and spontaneously. Higher glyphosate and TP removals were resulted from adsorption on the goethite produced, and higher TN removal was attributed to the PAD. High glyphosate and nutrients removal could keep a long term until the pyrrhotite in the Pyrr-CW was used up. The phosphorus (P) sequestered in the Pyrr-CW existed mainly in organic P, (Fe + Al)P and (Ca + Mg)P, and their order was (Fe + Al)P > organic P > (Ca + Mg)P. No heavy metal ions released from the Pyrr-CW. With higher and lasting removal rate, and lower cost, the Pyrr-CW is a promising technology for simultaneous glyphosate and nutrients removal from agricultural runoff and wastewater.


Assuntos
Nutrientes , Áreas Alagadas , Agricultura , Glicina/análogos & derivados , Nitrogênio , Fósforo , Eliminação de Resíduos Líquidos , Águas Residuárias
12.
Brain Res Bull ; 154: 9-20, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31626954

RESUMO

Hypertension is a common complication of metabolic abnormalities associated with cardiovascular system and characterized by sexual dimorphism in mammals. Fibroblast growth factor-21 (FGF-21) plays a critical role in metabolic-disorder related hypertension through the afferent loop of baroreflex. However, the gender difference in FGF-21-mediated blood pressure (BP) regulation via sexual dimorphic expression of FGFRs in the nodose (NG) and nucleus tractus solitarius (NTS) were not elucidated in physiological and genomic form of hypertension. The gene and protein expression of FGFRs were tested by qRT-PCR, immunoblotting and immunostaining; the serum level of FGF21 was tested using ELISA; The BP was monitored while FGF21 was nodose microinjected. The results showed that more potent BP reduction was confirmed in female vs. male rats by nodose microinjection of rhFGF-21 along with higher expression of FGFR2 and FGFR4 in the nodose compared with age-match male and ovariectomized (OVX) rats, rather than other receptor subtypes, which is consistent well with immunohistochemical analysis. Additionally, serum FGF-21 was significantly higher in female-WKY, and this level of FGF-21 was dramatically declined in spontaneous hypertensive rats (SHR) with significant down-regulation of FGFR1/R4 for male-SHR and FGFR2/FGFR4 for female-SHR, respectively. Apparently, high BP of SHR of either sex could be reduced by rhFGF-21 nodose microinjection. These data extends our current understanding that sexual-specific distribution/expression of FGF-21/FGFRs is likely to contribute at least partially to sexual dimorphism of baroreflex afferent function on BP regulation in rats. FGF-21-mdiated BP reduction sheds new light on clinical management of primary/genomic form of hypertension.

13.
Huan Jing Ke Xue ; 41(1): 224-231, 2020 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854923

RESUMO

In this paper, nutrient elements (N and P), heavy metals (Pb, Cu, Zn, Cd, Cr, Co, Ni, and Sn), and grain size in surface sediments of Baiyangdian Lake, northern China, are studied. We also analyze the spatial variation in elemental characteristics and undertaken a pollution risk assessment. By combining data with information on the river sediment characteristics, we use multivariate statistical methods to reveal the sources and variation of elements in sediments. The results showed that the average contents of heavy metals in surface sediments from the lake and associated rivers were higher than background values. Within the area of the lake, nutrient elements are relatively high in the northwest region but low in the southeast region, and heavy metals are relatively high in the middle of the lake but low in the southern and northern areas. The sequence of comprehensive pollution index (I) in sediments was Cd > Pb > Cr > Cu=Zn > Ni > Sn > Co, with Cd being assessed as severe pollution and other elements as moderate pollution, although severe pollution of Cr was found in the Zaolinzhuang area. The order of potential ecological risk coefficients (Eri) was determined as Cd > Pb > Cu > Cr > Ni > Zn, whereby Cd was associated with a strong potential ecological risk (except in the Caiputai area) and other elements were associated with a slight potential ecological risk. Differences in the lake sediment texture were found to be slight. Non-point source pollution after rivers flow into the lake was determined as the main reason for the spatial variation of elements in the surface sediments of Baiyangdian Lake, although point source pollution in the villages surrounding the lake should not be ignored, especially with respect to N, P, Pb, and Cr.


Assuntos
Monitoramento Ambiental , Sedimentos Geológicos/química , Lagos/química , Metais Pesados/análise , Poluentes Químicos da Água/análise , China , Medição de Risco , Rios/química
14.
Drug Des Devel Ther ; 13: 3823-3834, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31806933

RESUMO

Purpose: A previous study has identified that XRCC4-like factor (XLF) is a potential target to overcome resistance to 5-fluorouracil (5-Fu) and oxaliplatin (OXA) in colorectal cancer (CRC). The purpose of this study is to develop potent XLF inhibitors to chemoresistance in CRC. Methods: Virtual screening was adopted to identify novel XLF-binding compounds by initially testing 6800 molecules in Chemical Entities of Biological Interest library. Hit compounds were further validated by Western blot assay. Cell sensitivity to 5-Fu and OXA was measured using sulforhodamine B assay. The effect of XLF inhibitor on DNA repair efficiency was evaluated by comet assay, fluorescent-based nonhomologous end joining (NHEJ) and homologous recombination (HR) reporter assays. DNA-binding activity of NHEJ key factors was examined by chromatin fractionation assay. Results: We identified G3, a novel and potent XLF inhibitor (IC50 0.47±0.02 µM). G3 induced XLF protein degradation in CRC cells. Significantly, G3 improved cell sensitivity to 5-Fu and OXA in chemoresistant CRC cell lines. Mechanistically, G3 depleted XLF expression, severely compromised NHEJ efficiency by up to 65% and inhibited NHEJ key factor assembly on DNA. G3 also inhibited HR efficiency in a time-dependent manner. Conclusion: These results suggest that G3 overcomes 5-Fu and OXA resistance in CRC cells by inhibiting XLF expression. Thus, XLF is a promising target and its inhibitor G3 is a potential candidate for treatment of chemoresistant CRC patients.

15.
Nat Sci Sleep ; 11: 357-366, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819689

RESUMO

Purpose: Chronic intermittent hypoxia (CIH) contributes to the increased risk of cardiovascular diseases in obstructive sleep apnea (OSA). We previously reported the anti-apoptotic effects of estradiol (E2) on IH-exposed human umbilical vein endothelial cells (HUVECs). Herein, we employed a proteomic analysis to elucidate the mechanisms of the protective effects of E2 under IH exposure. Methods: HUVECs were divided into three groups: control, IH, and IH+E2 group. Isobaric tags for relative and absolute quantification (iTRAQ) were performed to compare protein profiles among the groups. Some of the identified proteins were validated by Western blotting. Results: A total of 185 proteins were differentially expressed in the IH+E2 group compared to the IH group. Bioinformatics analysis indicated that the effects of E2 may be linked to the regulation of cellular stress response. Among the differentially expressed proteins, we identified that serine-protein kinase ataxia telangiectasia mutated (ATM) and its downstream target, cellular inhibitor of apoptosis protein 1 (c-IAP1), were up-regulated by E2. We also observed that E2 decreased the level of cleaved caspase-3 and inhibited cell apoptosis in IH-exposed HUVECs. The inhibition of ATM abolished the anti-apoptotic effect of E2. Conclusion: The ATM-c-IAP1 pathway is involved in the cardioprotective effects of E2 in HUVECs exposed to IH.

16.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(6): 1786-1793, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-31839039

RESUMO

OBJECTIVE: To investigate the effect of reactive oxygen species (ROS) on GDC-0152-induced apoptosis and autophagy of acute promyelocytic leukemia cell line NB4. METHODS: Different concentrations of GDC-0152 combined with Z-VAD-FMK was applied to NB4 cells. Cell proliferation was detected by CCK8 method. Apoptosis rate, autophagy and ROS level were detected by flow cytometry. The autophagy was observed by Cyto-ID staining fluorescence microscopy, and flow cytometry were used to detect the fluorescence expression. The expression of autophagy-related protein LC3B was detected by Western blot. RESULTS: GDC-0152 increased proliferation inhibition rate and apoptosis rate in NB4 cells (P<0.05); GDC-0152 induced increase of ROS level of NB4 cells; GDC-0152 increased autophagy of NB4 cells that was found by Cyto-ID staining fluorescence microscopy and flow cytometry (P<0.05). Western blot showed that GDC-0152 increased LC3B expression in NB4 cells and promoted the conversion of LC3BI to LC3BII; as compared with GDC-0152 (100 ng/ml), GDC-0152 (100 ng/ml) combined with ROS inhibitor YCG063 (10 µmol/L) decreased apoptosis and autophagy (P<0.05). CONCLUSION: GDC-0152 inhibits cell proliferation by inducing apoptosis and autophagy of NB4 cells. ROS can promote GDC-0152-induced apoptosis and autophagy of NB4 cells.


Assuntos
Apoptose , Autofagia , Linhagem Celular Tumoral , Cicloexanos , Humanos , Leucemia Promielocítica Aguda , Pirróis , Espécies Reativas de Oxigênio
17.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(6): 2035-2038, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-31839080

RESUMO

Abstract  The patients with multiple myeloma are often accompanied by cardiovascular injuries, that not only related with age, but also with the disease itself and treatment. Timely detection and proper supervision of cardiovascular injuries in patients will reduce the mortality of patients with multiple myeloma. In this review, the pathophysiology, diagnosis and treatment of cardiovascular damages in patients with multiple myeloma are summarized briefly, so as to provide some references for clinical treatment and research.


Assuntos
Doenças Cardiovasculares , Mieloma Múltiplo , Humanos
18.
Huan Jing Ke Xue ; 40(11): 4953-4961, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854561

RESUMO

In situ sediments were collected at different sites of the Danjiangkou Reservoir using a columnar sediment sampler, and the release rate of N and P at the sediment-water interface was determined through static incubation experiments and the diffusion model of interstitial water molecules. The results showed that there was a significant difference in the release rate for N and P from sediments collected at five sampling sites. The release rates of NH4+-N and PO43--P under static incubation conditions were 13.07-24.88 mg·(m2·d)-1 and 3.06-6.02 mg·(m2·d)-1, whereas those estimated by Fick's Fist Law were 2.67-7.25 mg·(m2·d)-1 and 0.04-0.18 mg·(m2·d)-1, respectively. Overall, the release rates of N and P in the tributaries were 1.48 and 1.57 times higher than that in the reservoir, respectively, and they tended to decrease from the north to the south. The R/F values of NH4+-N and PO43--P were 3.43-4.98 and 29.67-72.88, respectively. The highest release rates of N and P were observed in the Guojiashan tributary for both methods. However, it was found that the release rates of N and P estimated by Fick's Fist Law were significantly lower than those obtained by the simulation method, indicating that the static incubation experiment with intact sediments allowed the release rates of N and P to be closer to the actual situation compared to the interstitial water molecule diffusion model.

19.
JCI Insight ; 4(24)2019 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-31852841

RESUMO

Clinical and preclinical studies show tissue-specific differences in tumorigenesis. Tissue specificity is controlled by differential gene expression. We prioritized genes that encode secreted proteins according to their preferential expression in normal lungs to identify candidates associated with lung cancer. Indeed, most of the lung-enriched genes identified in our analysis have known or suspected roles in lung cancer. We focused on the gene encoding neuron-derived neurotrophic factor (NDNF), which had not yet been associated with lung cancer. We determined that NDNF was preferentially expressed in the normal adult lung and that its expression was decreased in human lung adenocarcinoma and a mouse model of this cancer. Higher expression of NDNF was associated with better clinical outcome of patients with lung adenocarcinoma. Purified NDNF inhibited proliferation of lung cancer cells, whereas silencing NDNF promoted tumor cell growth in culture and in xenograft models. We determined that NDNF is downregulated through DNA hypermethylation near CpG island shores in human lung adenocarcinoma. Furthermore, the lung cancer-related DNA hypermethylation sites corresponded to the methylation sites that occurred in tissues with low NDNF expression. Thus, by analyzing the tissue-specific secretome, we identified a tumor-suppressive factor, NDNF, which is associated with patient outcomes in lung adenocarcinoma.

20.
Sheng Wu Gong Cheng Xue Bao ; 35(12): 2367-2385, 2019 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-31880142

RESUMO

Nanogenerator (triboelectric nanogenerator and piezoelectric nanogenerator) has experienced a rapid development since it was proposed. This technique can covert various mechanical energies into electric energy, including human motion energy, wind energy, acoustic energy and ocean energy. The converted electricity can be used for health monitoring and physiological function regulation, such as pulse detection, bioelectrical stimulation and cardiac pacing. This review summarizes the structure, working mechanism, output performance of nanogenerator and its latest progress in circulatory system, nervous system, biological tissue, sleep and rescue system. Additionally, a further analysis was also made on the application challenge of nanogenerator in clinical treatment. In the future, nanogenerator is expected to be an auxiliary power source, or even to replace battery to power medical electronic device and realize the self-powered health monitoring and physiological function regulation of human body.


Assuntos
Fontes de Energia Elétrica , Eletricidade , Humanos
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