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1.
Arch Pharm Res ; 2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32172437

RESUMO

Pulmonary arterial hypertension is a fatal disease, especially when it causes right heart failure (RHF). However, it is difficult to treat. It has been reported that trapidil (Tra) can improve the redox balance and cardiac conditions. In this study, we investigated the effect of Tra on RHF induced by monocrotaline (MCT) in rats. Male Wistar rats were treated with MCT or Tra. Treatment lasted 28 days, then rats were euthanized after echocardiography and catheterization. Subsequently, lungs and right ventricular myocardia were evaluated by hematoxylin and eosin, Masson, and TUNEL staining. Protein expression was detected by western blotting. We found remarkably expanded right ventricle end-diastolic volume, decreased partial pressure of oxygen (PaO2), increased partial pressure of carbon dioxide (PaCO2), right ventricular systolic pressure, mean pulmonary arterial pressure, lung/body weight, and liver/body weight in the RHF rat group, as well as increases in the apoptosis rate and the expression of endoplasmic reticulum stress (ERS)-related proteins. However, these changes were significantly inhibited by Tra. Our data suggested that inhibition of ERS is essential for improving RHF, and that therapeutic intervention of Tra in RHF rats works by reducing ERS.

2.
Clin Epigenetics ; 12(1): 17, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31964422

RESUMO

BACKGROUND: The 5-hydroxymethylcytosine (5hmC) DNA modification is an epigenetic marker involved in a range of biological processes. Its function has been studied extensively in tumors, neurodegenerative diseases, and atherosclerosis. Studies have reported that 5hmC modification is closely related to the phenotype transformation of vascular smooth muscle cells and endothelial dysfunction. However, its role in coronary artery disease (CAD) has not been fully studied. RESULTS: To investigate whether 5hmC modification correlates with CAD pathogenesis and whether 5hmC can be used as a biomarker, we used a low-input whole-genome sequencing technology based on selective chemical capture (hmC-Seal) to firstly generate the 5hmC profiles in the circulating cell-free DNA(cfDNA) of CAD patients, including stable coronary artery disease (sCAD) patients and acute myocardial infarction (AMI) patients. We detected a significant difference of 5hmC enrichment in gene bodies from CAD patients compared with normal coronary artery (NCA) individuals. Our results showed that CAD patients can be well separated from NCA individuals by 5hmC markers. The prediction performance of the model established by differentially regulated 5hmc modified genes were superior to common clinical indicators for the diagnosis of CAD (AUC = 0.93) and sCAD (AUC = 0.93). Specially, we found that 5hmC markers in cfDNA showed prediction potential for AMI (AUC = 0.95), which was superior to that of cardiac troponin I, muscle/brain creatine kinase, and myoglobin. CONCLUSIONS: Our results suggest that 5hmC markers derived from cfDNA can serve as effective epigenetic biomarkers for minimally noninvasive diagnosis and prediction of CAD.

3.
Environ Pollut ; 258: 113725, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31838383

RESUMO

The principal aim of this study was to understand the enrichment patterns of elements in water from typical coal mine and irrigation areas. For this study, samples of surface water, shallow water, and deep water were collected from Handan, Jining, and Heze cities and their surrounding areas in the central North China Plain. The results showed that the hydrochemical characteristics were dominated by Ca-Mg-Cl and Ca-HCO3. Elements in the studied surface water, including strontium, iron and boron, were anomalously enriched at levels more than 654, 294 and 134 times their global river water averages, respectively. The concentrations of elements in the studied area were influenced by both natural processes and anthropogenic sources, but the dominant origins of the anomalous enriched elements were bedrock weathering and soil leaching. The deep well water quality in the Handan coal mining area was good, while the poor-quality water samples in the study area were mainly distributed in the alluvial plain, which is characterized by Neogene-Quaternary sediments and aquifers. The measured hazard quotient and hazard index values indicate that the arsenic and nickel in the studied samples could pose a noncarcinogenic risk to the health of local residents, especially children. The leading source of the high arsenic levels is influenced by natural process. Monitoring plans for arsenic, iron, manganese, nitrate and other potentially harmful elements in surface water and groundwater and effective health education on pollution by these elements are essential.

4.
Cell Mol Immunol ; 17(2): 178-189, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30874628

RESUMO

Liver-resident NK cells are distinct from conventional NK cells and play an important role in the maintenance of liver homeostasis. How liver-resident NK cells participate in autoimmune cholangitis remains unclear. Here, we extensively investigated the impact of NK cells in the pathogenesis of autoimmune cholangitis utilizing the well-established dnTGFßRII cholangitis model, NK cell-deficient (Nfil3-/-) mice, adoptive transfer and in vivo antibody-mediated NK cell depletion. Our data demonstrated that disease progression was associated with a significantly reduced frequency of hepatic NK cells. Depletion of NK cells resulted in exacerbated autoimmune cholangitis in dnTGFßRII mice. We further confirmed that the DX5-CD11chi liver-resident NK cell subset colocalized with CD4+ T cells and inhibited CD4+ T cell proliferation. Gene expression microarray analysis demonstrated that liver-resident NK cells had a distinct gene expression pattern consisting of the increased expression of genes involved in negative regulatory functions in the context of the inflammatory microenvironment.

5.
Food Sci Nutr ; 7(10): 3302-3316, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31660144

RESUMO

The production of lactosucrose is hampered by the costly use of ß-fructofuranosidase, which shows poor stability and a low efficiency in transfructosylation activity. Immobilization could improve enzyme stability and realize the cyclic utilization at a reduced cost. In order to eliminate the by-product inhibition and improve the transfructosylation efficiency, ß-fructofuranosidase and glucose oxidase were co-immobilized by sol-gel encapsulation and the subsequent production efficiency of lactosucrose was investigated. The as-prepared immobilized bi-enzymes retained 85.39% of their initial activity at an enzyme concentration of 1.47 mg/g·sol during immobilization and showed great operational stability (maintaining 78.5% of their initial activity) after 15 consecutive reuses. The yield of lactosucrose synthesized by immobilized bi-enzymes reached 160.8 g/L under the optimized conditions, which was relatively higher than previous reported results. Moreover, the yield of lactosucrose synthesized by immobilized bi-enzymes was significantly improved as compared to that synthesized by immobilized ß-fructofuranosidase. HPLC and NMR spectrum results confirmed the presence of lactosucrose during immobilized bi-enzymes catalysis. Furthermore, a relatively high purity of lactosucrose was obtained (87.4% determined by HPLC) after separation with Diaion UBK535 calcium ester resin, and the optimal conditions for separation of lactosucrose were investigated. These results indicated that the co-immobilization of ß-fructofuranosidase-glucose oxidase was helpful to improve the production of lactosucrose with low costs, which can be used in continuous lactosucrose production in food industry in advantages of high stability and reusability. And the as-prepared lactosucrose with high purity can be applied to many kinds of food as functional additives.

6.
Front Pharmacol ; 10: 1040, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31607907

RESUMO

Background: Metabolic syndrome (MS) is one of the major causes of coronary artery diseases (CAD). Gut microbiome diversity and its natural fermentation products are not only correlated with MS and CAD, but their correlations also appear to be stronger than the associations with traditional risk factors. Therefore, the aim of this study was to provide a new potential pathway for the natural fermentation product butyrate to improve MS and to examine whether it is associated with serum metabolic profiles and gut flora composition. Methods: C57BL/6J mice fed a high-fat diet (HFD) were treated with 400 mg/kg of sodium butyrate for 16 weeks. Blood and fecal samples were collected, and the metabolite concentrations and 16s rRNA were measured with liquid chromatography-MS and Illumina platform, respectively. The plasma differential metabolites and gut microbiome composition were analyzed with XCMS online and QIIME 2, respectively. Results: Gut microbiome-derived butyrate reduced glucose intolerance and insulin resistance, resisting HFD-induced increase in the relative abundance of f_Lachnospiraceae, f_Rikenellaceae, and f_Paraprevotellaceae. Meanwhile, sodium butyrate increased the levels of α-linolenate, all-trans-retinal, resolvin E1, and leukotriene in the plasma, and the differential pathways showed enrichment in mainly resolvin E biosynthesis, histidine degradation, lipoxin biosynthesis, and leukotriene biosynthesis. Moreover, sodium butyrate increased the levels of phosphorylated-adenosine 5'-monophosphate-activated protein kinase (p-AMPK) and facilitated glucose transporter member 4 (GLUT4) in the adipose tissue. Conclusion: Butyrate can induce AMPK activation and GLUT4 expression in the adipose tissue, improving cardiovascular disease (CVD)-related metabolic disorder, resisting HFD-induced gut microbiome dysbiosis, and promoting resolvin E1 and lipoxin biosynthesis. Oral supplement of the natural fermentation product butyrate can be a potential strategy for preventing CVD.

7.
J Craniofac Surg ; 30(7): 2253-2256, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31513037

RESUMO

This paper describes the application of a modified digital surgical guide, an occlusal template and a pre-formed reconstruction titanium plate in the open reduction and internal fixation of a bilateral mandibular fracture. Bilateral mandibular fracture is a commonly encountered simple type of fracture. However, even for an experience surgeon, achieving precise treatment remains a challenge due to the movable temporomandibular joint, unstable fractured segments, the difficulty in forming a reconstruction plate, and the lack of an effective stabilizing and locating device. In this case, the surgeon used a specially-designed modified guide together with a reconstruction plate and an occlusal template to treat a bilateral mandible fracture, effectively improving the accuracy and the medical outcome of the operation, saving operation time and reducing postoperative complications.


Assuntos
Fraturas Mandibulares/cirurgia , Reconstrução Mandibular , Adulto , Placas Ósseas , Fixação Interna de Fraturas , Humanos , Masculino , Fraturas Mandibulares/diagnóstico , Reconstrução Mandibular/instrumentação , Duração da Cirurgia , Complicações Pós-Operatórias , Cirurgia Assistida por Computador , Articulação Temporomandibular/cirurgia , Titânio
8.
Int J Mol Med ; 44(3): 857-870, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31257525

RESUMO

Although miRNAs have been implicated in the osteogenic differentiation of stem cells, their role in bone repair and reconstruction in tissue­engineered bone grafts remains unclear. We previously reported that microRNA (miR)­26a­5p inhibited the osteogenic differentiation of adipose­derived mesenchymal stem cells (ADSCs), and that antimiR­26a­5p exerted the opposite effect. In the present study, the role of miR­26a­5p­ and antimiR­26a­5p­modified ADSCs combined with biphasic calcium phosphate (BCP) scaffolds was evaluated in a rat femur defect model. The aim of the present study was to improve the understanding of the role of miR­26a­5p in bone regeneration in vivo, as well as to provide a new method to optimize the osteogenic ability of BCPs. ADSCs were infected with Lv­miR­26a­5p, Lv­miR­NC, Lv­antimiR­26a­5p or Lv­antimiR­NC respectively, and then combined with BCP scaffolds to repair rat femoral defects. Using X­rays, micro­computed tomography and histology at 2, 4, and 8 weeks postoperatively, the quantity and rate of bone regeneration were analyzed, revealing that they were the highest in animals treated with antimiR­26a­5p and the lowest in the miR­26a­5p treatment group. The expression levels of osteocalcin, collagen I, Runt­related transcription factor 2, Wnt family member 5A and calmodulin­dependent protein kinase II proteins were positively correlated with the bone formation rate. Taken together, the present results demonstrated that miR­26a­5p inhibited bone formation while antimiR­26a­5p accelerated bone formation via the Wnt/Ca2+ signaling pathway. Therefore, antimiR­26a­5p­modified ADSCs combined with BCP scaffolds may be used to construct an effective tissue­engineering bone graft for bone repair and reconstruction.


Assuntos
Regeneração Óssea , Fêmur/metabolismo , Hidroxiapatitas/metabolismo , MicroRNAs/genética , Animais , Cálcio/metabolismo , Sinalização do Cálcio , Proliferação de Células , Feminino , Fêmur/diagnóstico por imagem , Fêmur/patologia , Hidroxiapatitas/farmacologia , Processamento de Imagem Assistida por Computador , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/metabolismo , Osteogênese , Ratos , Via de Sinalização Wnt , Microtomografia por Raio-X
9.
Int J Mol Sci ; 20(10)2019 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-31108845

RESUMO

Anthracnose is a major leaf disease in tea plant induced by Colletotrichum, which has led to substantial losses in yield and quality of tea. The molecular mechanism with regards to responses or resistance to anthracnose in tea remains unclear. A de novo transcriptome assembly dataset was generated from healthy and anthracnose-infected leaves on tea cultivars "Longjing-43" (LJ43) and "Zhenong-139" (ZN139), with 381.52 million pair-end reads, encompassing 47.78 billion bases. The unigenes were annotated versus Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), National Center for Biotechnology Information (NCBI) non-redundant protein sequences (Nr), evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) and Swiss-prot. The number of differential expression genes (DEGs) detected between healthy and infected leaves was 1621 in LJ43 and 3089 in ZN139. The GO and KEGG enrichment analysis revealed that the DEGs were highly enriched in catalytic activity, oxidation-reduction, cell-wall reinforcement, plant hormone signal transduction and plant-pathogen interaction. Further studies by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and high-performance liquid chromatography (HPLC) showed that expression of genes involved in endogenous salicylic acid biosynthesis and also accumulation of foliar salicylic acid are involved in the response of tea plant to anthracnose infection. This study firstly provided novel insight in salicylic acid acting as a key compound in the responses of tea plant to anthracnose disease. The transcriptome dataset in this study will facilitate to profile gene expression and metabolic networks associated with tea plant immunity against anthracnose.


Assuntos
Camellia sinensis/genética , Colletotrichum/patogenicidade , Perfilação da Expressão Gênica/métodos , Redes Reguladoras de Genes , Camellia sinensis/metabolismo , Camellia sinensis/microbiologia , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Sequenciamento de Nucleotídeos em Larga Escala , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Folhas de Planta/genética , Proteínas de Plantas/genética , Ácido Salicílico/metabolismo
10.
Sci Rep ; 9(1): 6586, 2019 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-31036856

RESUMO

Data from the Qinling Orogenic Belt in China indicate that a strong magmatic-volcanic event on the Snowball Earth during the Cryogenian age (approximately 720-635 million years ago) was followed by a dynamic period of accelerated evolution of early life through the Ediacaran period. The studied volcanics of the Cryogenian Yaolinghe group are mainly represented by andesite, dacite and rhyolite, with minor amounts of basalt, trachy andesite and trachyte towards the top, which formed in the environment of an active island arc related to a continental margin. Compared with average felsic volcanics, the studied Cryogenian marine volcanic strata are enriched (1.5-30.6 times) in Co, Cr, Bi, Ni, Se, Ga, As, Cu, Ba, V, and Zn. Elemental concentrations (P, Cd, Co, Ni, and Se) of the studied volcanics are more than 5-26.4 times those in the contemporaneous Liantuo tillite. We propose that Cryogenian magmatic and volcanic activity increased the flux of some trace nutritional elements into the oceans which possibly provided essential nutrients for the development of early life.

11.
J Transl Med ; 17(1): 64, 2019 02 28.
Artigo em Inglês | MEDLINE | ID: mdl-30819183

RESUMO

BACKGROUND: Osteopenia have been well documented in adolescent idiopathic scoliosis (AIS). Adiponectin has been shown to be inversely proportional to body mass index and to affect bone metabolism. However, the circulating levels of adiponectin and the relationship between adiponectin and low bone mass in AIS remain unclear. METHODS: A total of 563 AIS and 281 age-matched controls were recruited for this study. Anthropometry and bone mass were measured in all participants. Plasma adiponectin levels were determined by enzyme-linked immunosorbent assay (ELISA) in the AIS and control groups. An improved multiplex ligation detection reaction was performed to study on single nucleotide polymorphism. Facet joints were collected and used to measure the microstructure, the expression of RANKL, OPG, osteoblast-related genes, inflammatory factors, adiponectin and its receptors by qPCR, western blotting and immunohistochemistry. Furthermore, primary cells were extracted from facet joints to observe the reaction after adiponectin stimulation. RESULTS: Compared with the controls, lower body mass index and a marked increase in circulating adiponectin were observed in AIS osteopenia (17.09 ± 1.09 kg/m2 and 21.63 ± 10.30 mg/L). A significant difference in the presence of rs7639352was detected in the AIS osteopenia, AIS normal bone mass and control groups. The T allele showed a significant higher proportion in AIS osteopenia than AIS normal bone mass and control groups (41.75% vs 31.3% vs 25.7%, p < 0.05). micro-CT demonstrated that the AIS convex side had a significant lower bone volume than concave side. RNA and protein analyses showed that in cancellous bone, higher RANKL/OPG and adipoR1 levels and lower runx2 levels were observed, and in cartilage, higher adipoR1 and IL6 levels were observed in AIS. Furthermore, convex side had higher RANKL/OPG, IL6 and adipoR1 than concave side. Compared with normal primary cells, convex side primary cells showed the most acute action, and concave side primary cells showed the second-most acute action when exposed under same adiponectin concentration gradient. CONCLUSION: Our results indicated that high circulating adiponectin levels may result from gene variations in AIS osteopenia. Adiponectin has a negative effect on bone metabolism, and this negative effect might be mediated by the ADR1-RANKL/OPG and ADR1-IL6 pathways.

12.
Cancer Res ; 79(13): 3431-3444, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30902796

RESUMO

The Aurora A inhibitor alisertib shows encouraging activities in clinical trials against advanced breast cancer. However, it remains unclear whether and how the inflammatory microenvironment is involved in its efficacy. Here, we demonstrated that inhibition of Aurora A directly reshaped the immune microenvironment through removal of tumor-promoting myeloid cells and enrichment of anticancer T lymphocytes, which established a tumor-suppressive microenvironment and significantly contributed to the regression of murine mammary tumors. Mechanistically, alisertib treatment triggered apoptosis in myeloid-derived suppressor cells (MDSC) and macrophages, resulting in their elimination from tumors. Furthermore, alisertib treatment disrupted the immunosuppressive functions of MDSC by inhibiting Stat3-mediated ROS production. These alterations led to significant increases of active CD8+ and CD4+ T lymphocytes, which efficiently inhibited the proliferation of tumor cells. Intriguingly, alisertib combined with PD-L1 blockade showed synergistic efficacy in the treatment of mammary tumors. These results detail the effects of Aurora A inhibition on the immune microenvironment and provide a novel chemo-immunotherapy strategy for advanced breast cancers. SIGNIFICANCE: These findings show that inhibition of Aurora A facilitates an anticancer immune microenvironment, which can suppress tumor progression and enhance anti-PD-L1 therapy in breast cancer.See related commentary by Rivoltini et al., p. 3169.


Assuntos
Antígeno B7-H1 , Neoplasias da Mama , Animais , Aurora Quinase A , Humanos , Camundongos , Células Mieloides , Receptor de Morte Celular Programada 1 , Microambiente Tumoral
13.
Molecules ; 24(5)2019 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-30857144

RESUMO

There is epidemiological evidence showing that drinking green tea can lower the risk of esophageal cancer (EC). The effect is mainly attributed to tea polyphenols and their most abundant component, (-)-epigallocatechin-3-gallate (EGCG). The possible mechanisms of tumorigenesis inhibition of EGCG include its suppressive effects on cancer cell proliferation, angiogenesis, DNA methylation, metastasis and oxidant stress. EGCG modulates multiple signal transduction and metabolic signaling pathways involving in EC. A synergistic effect was also observed when EGCG was used in combination with other treatment methods.


Assuntos
Catequina/análogos & derivados , Neoplasias Esofágicas/tratamento farmacológico , Antineoplásicos/química , Antineoplásicos/farmacologia , Catequina/química , Catequina/farmacologia , Proliferação de Células/efeitos dos fármacos , Metilação de DNA/efeitos dos fármacos , Humanos , Polifenóis/química , Transdução de Sinais/efeitos dos fármacos , Chá
14.
Thorac Cancer ; 10(4): 665-675, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30756482

RESUMO

BACKGROUND: Annexin 1 (ANXA1) expression is associated with the malignant tumor phenotype, making it an attractive therapeutic target. However, little is known about the regulation of ANXA1 in non-small cell lung cancer (NSCLC). METHODS: We investigated the biological roles of ANXA1 in tumor growth, migration, and invasion, and explored the possibility of ANXA1 as a potential therapeutic target for the treatment of NSCLC. RESULTS: Our findings revealed that ANXA1 enhanced nuclear factor (NF)-κB activation in NSCLC cells by interaction with inhibitor of NF-κB kinase complex subunit, IKKγ. We also found that NF-κB could negatively regulate microRNA (miR)-26a, and miR-26a was regulated through the ANXA1-NF-κB regulatory pathway. NF-κB activation negatively regulated by miR-26a was confirmed in NSCLC. CONCLUSION: Together, these results provide evidence of the mechanisms of the ANXA1-NF-κB-miR-26a regulatory pathway in the invasion and migration in NSCLC.

15.
Nat Cell Biol ; 21(2): 214-225, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30692626

RESUMO

The serine/threonine kinase Akt plays a central role in cell proliferation, survival and metabolism, and its hyperactivation is linked to cancer progression. Here we report that Akt undergoes K64 methylation by SETDB1, which is crucial for cell membrane recruitment, phosphorylation and activation of Akt following growth factor stimulation. Furthermore, we reveal an adaptor function of histone demethylase JMJD2A, which is important for recognizing Akt K64 methylation and recruits E3 ligase TRAF6 and Skp2-SCF to the Akt complex, independently of its demethylase activity, thereby initiating K63-linked ubiquitination, cell membrane recruitment and activation of Akt. Notably, the cancer-associated Akt mutant E17K displays enhanced K64 methylation, leading to its hyper-phosphorylation and activation. SETDB1-mediated Akt K64 methylation is upregulated and correlated with Akt hyperactivation in non-small-cell lung carcinoma (NSCLC), promotes tumour development and predicts poor outcome. Collectively, these findings reveal complicated layers of Akt activation regulation coordinated by SETDB1-mediated Akt K64 methylation to drive tumorigenesis.


Assuntos
Carcinogênese/metabolismo , Metiltransferases de Proteína/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ubiquitinação , Células A549 , Animais , Carcinogênese/genética , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Feminino , Células HEK293 , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , Lisina/genética , Lisina/metabolismo , Metilação , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Knockout , Camundongos Nus , Células NIH 3T3 , Metiltransferases de Proteína/genética , Proteínas Proto-Oncogênicas c-akt/genética , Transplante Heterólogo
16.
J Sci Food Agric ; 99(4): 1740-1747, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30226268

RESUMO

BACKGROUND: In order to supply adequate dietary protein for chronic kidney disease (CKD) patients while simultaneously controlling phosphorus intake, a novel method was developed for the preparation of low-phosphorus egg yolk protein (LPYP) using alkaline protease auxiliary dephosphorization. In addition, the physicochemical properties of LPYP were studied. RESULTS: In comparison with raw egg yolk protein (RYP) and defatted egg yolk protein (DFYP), LPYP was found to exhibit differences in amino acid (AA) composition, protein secondary structure, surface hydrophobicity, solubility and emulsion stability. It was observed that dephosphorization improved the AA composition, soluble protein content and dissolution stability of egg yolk protein. In addition, phosphate groups were found to impose a critical influence on the emulsion stability and particle size distribution. The final phosphorus to protein mass ratio (P/Pro) of LPYP was 5.64, which met the requirements of a protein diet for CKD patients. The FAO/WHO mode closeness and stability coefficient were 0.958 and 98.62% respectively. CONCLUSION: LPYP can be effectively obtained by alkaline protease hydrolysis and subsequent alkali dephosphorization. The prepared LPYP can be considered to be a type of safe and suitable protein resource for CKD patients. © 2018 Society of Chemical Industry.


Assuntos
Proteínas do Ovo/química , Gema de Ovo/química , Manipulação de Alimentos/métodos , Fósforo/análise , Aminoácidos/análise , Animais , Proteínas de Bactérias/química , Biocatálise , Galinhas , Ovos/análise , Endopeptidases/química , Solubilidade
17.
J Chromatogr A ; 1587: 146-154, 2019 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-30580960

RESUMO

In metabolomics studies, metabolic pathway recognition (MPR) is performed by software tools to screen out the significant pathways disturbed by diseases or reinstated by drugs. To achieve MPR, the significantly changed metabolites determined in different biospecimens (e.g. plasma and urine) are analyzed either independently (metabolites from each biospecimen as a dataset) or integratively (metabolites from all biospecimens as a dataset). However, whether the choice of these two processing approaches affects the results of MPR remains unknown. In this study, this issue was addressed by selecting evaluation of the effects of the herbal medicine Rehmanniae Radix (RR) on anemia and adrenal fatigue by UPLC-QTOF-MS/MS-based metabolomics as an example. The significant pathways disturbed by the modeling of anemia and adrenal fatigue and those reinstated by treatments with raw and processed RR were recognized using MetPA software tool (MetaboAnalyst 3.0), and compared by independent and integrative processing of the significantly changed metabolites determined in plasma and urine. The results showed that the two processing approaches could yield different impact values of pathways and thereby recognize different significant pathways. The differences appear to happen more easily when metabolites from different biospecimens shared the same metabolic pathway. Such pathway could be recognized as a significant pathway by integrative processing but could be excluded by independent processing due to the converged and dispersed importance contributions of the involved metabolites to MPR in the two processing approaches. This issue should concern researchers because MPR is crucial not only to understanding metabolomics data but also to guiding subsequent mechanistic research.


Assuntos
Bases de Dados como Assunto , Redes e Vias Metabólicas , Metabolômica/métodos , Plasma/metabolismo , Urina/química , Anemia/sangue , Anemia/metabolismo , Anemia/urina , Animais , Bioensaio , Humanos , Masculino , Metaboloma , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem
18.
Chin J Traumatol ; 21(5): 281-286, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30342986

RESUMO

Patients suffering from zygomatic complex fractures always present facial deformity and dysfunctions, and thereafter develop psychological and physiological problems. It is really hard to get an ideal prognosis for the zygomatic complex fractures because of the complicated anatomical structures. Computer-assisted surgery techniques, as the new emerging auxiliary methods, can optimize the surgical protocol, predict operation outcomes, and improve the accuracy and quality of the operation. Meanwhile the postoperative complications can be reduced effectively. This review aims to provide a comprehensive overview of the application of computer-assisted surgery techniques in the management of zygomatic complex fractures.


Assuntos
Fixação Interna de Fraturas/métodos , Imagem Tridimensional , Cirurgia Assistida por Computador/métodos , Fraturas Zigomáticas/diagnóstico por imagem , Fraturas Zigomáticas/cirurgia , Adulto , China , Feminino , Consolidação da Fratura/fisiologia , Humanos , Escala de Gravidade do Ferimento , Masculino , Pessoa de Meia-Idade , Prognóstico , Procedimentos Cirúrgicos Robóticos/métodos
19.
Mol Ther Nucleic Acids ; 12: 741-750, 2018 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-30121551

RESUMO

The role of p53 in renal fibrosis is still controversial, and its underlying mechanisms remain not clear. Here, we showed that the pharmacological inhibition and genetic deletion of p53 in proximal tubular cells can attenuate renal dysfunction, tubular epithelial disruption, and interstitial fibrosis in db/db and STZ-induced diabetic nephrology (DN) mice. In human renal proximal tubule (human kidney 2 [HK-2]) cells, inhibition of p53 by PIF reduced the high glucose (HG)-induced extracellular matrix (ECM) accumulation and reversed the inhibitory effect of HG on mRNA expression levels of lncRNA zinc finger E-box binding homeobox1-antisense RNA 1 (ZEB1-AS1) and ZEB1. Interestingly, our results demonstrated that both lncRNA ZEB1-AS1 and ZEB1 exhibited an anti-fibrotic role, while ZEB1 is positively regulated by lncRNA ZEB1-AS1 during HG treatment. Mechanistically, lnc ZEB1-AS1 bound directly to H3K4 methyltransferase myeloid and lymphoid or mixed-lineage leukemia 1 (MLL1) and promoted H3K4me3 histone modification on ZEB1 promoter, which was reduced by HG treatment. ChIP analysis indicated the binding of p53 to the promoter region of lnc ZEB1-AS1. Furthermore, the findings were verified by the kidney biopsy samples from patients with DN. Taken all together, our results suggest that p53 may be a therapeutic target for renal fibrosis in DN.

20.
Clin Breast Cancer ; 18(5): e985-e995, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-29983379

RESUMO

BACKGROUND: Contrast-enhanced spectral mammography (CESM) is a new image examination technology that has developed over the past few years. As CESM technology keeps improving, a current meta-analysis review is needed to systematically evaluate the potential diagnostic value of CESM. METHODS: A total of 18 studies were included in the review. Sensitivity, specificity, and other important parameters of CESM accuracy for breast cancer diagnosis were pooled and analyzed using random-effects models. Summary receiver operating characteristic curves were calculated for overall accuracy estimation. RESULTS: The summary estimates for CESM in the diagnosis of breast cancer were as follows: the pooled sensitivity and specificity were 0.89 (95% confidence interval [CI], 0.88-0.91) and 0.84 (95% CI, 0.82-0.85), respectively. Positive likelihood ratio was 3.73 (95% CI, 2.68-5.20), negative likelihood ratio was 0.10 (95% CI, 0.06-0.15), and diagnostic odds ratio was 71.36 (95% CI, 36.28-140.39). The area under the curve was 0.96 (standard error = 0.011). CONCLUSION: CESM has a high diagnostic accuracy for evaluating breast cancer and can be considered as a useful test for initial assessment of breast lesions.


Assuntos
Neoplasias da Mama/diagnóstico por imagem , Mamografia , Intensificação de Imagem Radiográfica , Mama/diagnóstico por imagem , Mama/patologia , Neoplasias da Mama/patologia , Detecção Precoce de Câncer , Feminino , Humanos , Mamografia/estatística & dados numéricos , Razão de Chances , Sensibilidade e Especificidade
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