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1.
Neurogastroenterol Motil ; : e14020, 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-33112027

RESUMO

BACKGROUND: Cisplatin is an antineoplastic drug known to produce intense vomiting, gastric dysmotility, and peripheral neuropathy. Monosodium glutamate (MSG) is a flavor enhancer with prokinetic properties potentially useful for cancer patients under chemotherapy. Our aim was to test whether MSG may improve gastrointestinal motor dysfunction and other adverse effects induced by repeated cisplatin in rats. METHODS: Male Wistar rats were exposed or not to MSG (4 g L-1 ) in drinking water from week 0 to 1 week after treatment. On the first day of weeks 1-5, rats were treated with saline or cisplatin (2 mg kg-1  week-1 , ip). Gastrointestinal motility was measured by radiological methods after first and fifth administrations, as well as 1 week after treatment finalization. One week after treatment, the threshold for mechanical somatic sensitivity was recorded. Finally, samples of stomach, terminal ileum and kidneys were evaluated in sections using conventional histology. The myenteric plexus was immunohistochemically evaluated on distal colon whole-mount preparations. KEY RESULTS: Monosodium glutamate prevented the development of cisplatin-induced neuropathy and partially improved intestinal transit after the fifth cisplatin administration with little impact on gastric dysmotility. MSG did not improve the histological damage of gut wall, but prevented the changes induced by cisplatin in the colonic myenteric plexus. CONCLUSION AND INFERENCES: Our results suggest that MSG can improve some dysfunctions caused by anticancer chemotherapy in the gut and other systems, associated, at least partially, with neuroprotectant effects. The potentially useful adjuvant role of this food additive to reduce chemotherapy-induced sequelae warrants further evaluation.

2.
Nutrients ; 11(6)2019 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-31234581

RESUMO

The bioaccessibility, metabolism, and excretion of lipids composing spent coffee grounds (SCGs) were investigated. An analysis of mycotoxins and an acute toxicity study in rats were performed for safety evaluation. Total fat, fatty acids, and diterpenes (cafestol and kahweol) were determined in SCGs and their digests obtained in vitro. A pilot repeated intake study was carried out in Wistar rats using a dose of 1 g SCGs/kg b.w. for 28 days. Fat metabolism was evaluated by analysis of total fat, cholesterol, and histology in liver. The dietary fiber effect of SCGs was measured radiographically. The absence of mycotoxins and toxicity was reported in SCGs. A total of 77% of unsaturated fatty acids and low amounts of kahweol (7.09 µg/g) and cafestol (414.39 µg/g) were bioaccessible after in vitro digestion. A significantly lower (p < 0.1) accumulation of lipids in the liver and a higher excretion of these in feces was found in rats treated with SCGs for 28 days. No lipid droplets or liver damage were observed by histology. SCGs acutely accelerated intestinal motility in rats. SCGs might be considered a sustainable, safe, and healthy food ingredient with potential for preventing hepatic steatosis due to their effect as dietary fiber with a high fat-holding capacity.


Assuntos
Coffea/metabolismo , Diterpenos/metabolismo , Ácidos Graxos/metabolismo , Sementes/metabolismo , Animais , Disponibilidade Biológica , Biotransformação , Coffea/toxicidade , Diterpenos/administração & dosagem , Ácidos Graxos/administração & dosagem , Fezes/química , Feminino , Motilidade Gastrointestinal/efeitos dos fármacos , Eliminação Intestinal , Fígado/metabolismo , Masculino , Projetos Piloto , Ratos Wistar , Sementes/toxicidade , Fatores de Tempo
3.
Eur. j. anat ; 22(6): 471-481, nov. 2018. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-182114

RESUMO

In this paper we describe a SEM block-face technique in which block faces of large dimensions can be examined in a high-resolution SEM under high vacuum. The results of different tissue contrast methods have been studied and, in addition to osmium, potassium permanganate has been used as a staining medium for the first time in BFSEM. The study also examined the effects of uranyl acetate and phosphotungstic acid. The following organs of adult albino rats were examined: colonic mucosa, spinal ganglion, anterior pituitary gland and exocrine pancreas.Six preparation protocols, referred to here as treatments, were applied and evaluated according to three criteria: 1st the visual quality of the digital images, 2nd the measurements of the signal-noise ratio (SNR) of the digital images with and without beam deceleration (BD), and 3rd the X-ray microanalysis of samples, treated according to the 6 proposed protocols, demonstrating the presence and relative quantity of the elements used to stain the cellular structures, enabling visualisation with the electron microscope. In conclusion, it can be said that treatments with osmium produced better results than those containing potassium permanganate. Treatments with the addition of thiocarbohydrazide (TCH) considerably increased the osmium deposits (ligand effect) and proved highly effective. Finally, it should be noted that the method proposed, called here 2D BFSEM, can be very useful not only in histology but also in histo-pathology, for example in the study of biopsies and - last but not least - in embryology: all these are situations in which it is important to avoid a loss of material due to preparation exigencies


No disponible


Assuntos
Animais , Masculino , Feminino , Ratos , Colo/anatomia & histologia , Espectrometria por Raios X/instrumentação , Microscopia Eletrônica/métodos , Espectrometria por Raios X/métodos , Meios de Contraste , Osmio/uso terapêutico
4.
Eur. j. anat ; 22(1): 1-15, ene. 2018. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-170476

RESUMO

This study proposes the use of isolated colonic mucosa as a "scaffold" for cell cultures and potentially for tissue reconstruction. The main goal of this study was to obtain complete decellularization of the specimens while preserving the superficial basement membrane (BM) as a place for cell attachment and growth. This decellularization technique uses a chelating agent in combination with mechanical vibration, followed by detergents and DNase. The grade of decellularization achieved is assessed by counting the number of cell nuclei stained with propidium iodide (PI). BM marker proteins such as collagen IV, laminin and perlecan were detected by immunohistochemistry (ICC). Transmission (TEM) and scanning electron microscopy (SEM) were used to examine the BM ultrastructure and surface topography. The results show that the protocol used is suitable for rat colonic mucosa. During the process, material of the lamina lucida (LL) was partly removed from the BM, whereas the lamina densa (LD) seems to have remained unchanged. The BM had become thinner than the control specimens. The nanotopography of the BM surface is characterized by globules of 34-60 nm in diameter. Human fetal fibroblasts were successfully cultured on this substrate confirming that cells can adhere to and grow on this substrate, at least for the particular cell line used. It can be said that the colonic mucosa is an interesting substrate for in vitro stud-ies with cells and presumably also for tissue reconstruction


No disponible


Assuntos
Animais , Ratos , Mucosa Intestinal/citologia , Mucosa Intestinal/diagnóstico por imagem , Imuno-Histoquímica/métodos , Membrana Basal/citologia , Membrana Mucosa/diagnóstico por imagem , Membrana Mucosa , Colo/citologia , Colo/diagnóstico por imagem , Microscopia Eletrônica/métodos , Projetos de Pesquisa
5.
Eur. j. anat ; 20(2): 121-130, abr. 2016. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-152868

RESUMO

Platelets are blood cellular components involved in hemostatic processes and thrombus formation. Activation and inhibition of platelets result in an increase in morphological changes and a significant reduction in adhesion. There are several approaches towards the determination of the functional status of platelets, based on criteria such as cell adhesion, molecular changes at the cell surface, etc. In recent years, microfluidic devices have been introduced to mimic conditions proper to the vascular system, and so emulate thrombus formation in vivo. This study presents a microchip, the Thrombi Chip® , which is partially fitted with fluidic properties. This microchip has various types of micro-channels into which the platelets are inserted and, after drug treatment, the investigation is completed with the examination of the chip under an invert light microscope. For microscopy, cells were labeled with FCDA (human platelets) and Rho6G (mouse platelets). Counts and morphometric measurements of the adhered cells were carried out using digital images. To validate the results obtained with the microchip, the fractions of mice platelets were investigated with flow cytometry as well. Scanning electron microscopy was used to examine the morphological changes related to activation and inhibition in human platelets. The results show that, with this microchip, activation and inhibition of platelets can be detected. Flow cytometry studies largely confirm the microchip results. Certain variability in the results observed in human platelets is considered normal, as donors were randomized. In this respect the mouse platelets were much more uniform. Measurements with the microchip require that the sample be divided into three groups: control, activated and inhibited, resulting in a set of data, which, after respective evaluation, provides activity profiles, giving information on the status and response capacity of a sample. Such profiles could have diagnostic relevance and therefore be useful in a clinical context, for example in the monitoring of the effects of short- and long-term treatment of patients, as well as to test new drugs


No disponible


Assuntos
Humanos , Plaquetas/citologia , Técnicas Analíticas Microfluídicas/métodos , Ativação Plaquetária/fisiologia , Plaquetas/fisiologia , Hemostasia/fisiologia , Inibidores da Agregação de Plaquetas/análise , Trombose/fisiopatologia
6.
Arch. med ; 13(2): 167-180, 30/dez. 2013.
Artigo em Espanhol | LILACS | ID: lil-707520

RESUMO

Objetivo: Evaluar los progresos del crecimiento y del desarrollo durante el primer trimestre de vida según lo estipulado en la Resolución 0412/00 del Ministerio de la Protección social de la República de Colombia, y la adherencia a la 1° y 2° consulta posnatal por parte de las madres. Materiales y métodos: Estudio de corte transversal analítico, en una población de 1 229 recién nacidos, cuyo parto fue atendido en las clínicas de ASSBSALUD E.S.E (Manizales-Colombia) sede San Cayetano entre los años 2011 y 2012. Se analizaron las historias clínicas para extraer de allí los variables necesarias par a el estudio. Resultados: Al primer control posnatal asistieron el 83,2% de los recién nacidos, con una edad promedio de 3,4 días, al 2° el 44,6%, con una edad promedio de 3,4 meses, el 76,7% de las madres dieron lactancia materna exclusiva a sus bebés, el 6,1% no tenían plan de inmunización completo, 10% con patologías congénitas, Osteo-muscular en el 28,3%, 44,3% de patología adquirida, en el 53,5% respiratoria, sífilis congénita en el o,2%, no se encontraron casos de ictericia.Básicamente asistió a controles la población urbana, la que había asistido a más controles prenatales, y cuando el bebé tenía alguna patología, congénita o adquirida. Conclusiones: La alta inasistencia, sobre todo en l a 2° consulta posnacimiento,dificulta el seguimiento del progreso del crecimiento y desarrollo del bebé durante el primer trimestre de vida en esta población.


Assuntos
Cuidado da Criança , Crescimento e Desenvolvimento , Recém-Nascido , Pediatria
7.
Photomed Laser Surg ; 29(2): 75-81, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20969437

RESUMO

OBJECTIVE: Development of a robotic ArF excimer laser device with a three-dimensional (3D) pattern scanning sensor for the controlled de-epithelization of live mouse and xenografted epidermis. SIGNIFICANCE: The animal model could be adapted to humans for automated, minimally invasive de-epithelization of cutaneous areas and therefore is of interest for cutaneous gene therapy research. MATERIALS AND METHODS: Ablation thresholds of mouse, porcine, and human skin were measured by acoustic detection methods. These ablation thresholds were used as initial parameters for dosimetry measurements. De-epithelization of live mouse and xenografted epidermis was performed by laser ablation (ArF excimer laser, λ = 193 nm, t(p) = 20 nsec). The rectangular shape of the laser spot and a robotic arm displacement incorporating a three-dimensional patter scanning sensor allowed a polygonal tile floor irradiation of a 2-cm-diameter area. Ablated epidermis was subjected to histology. RESULTS: SCID and nude mouse skin did not entirely reflect the de-epithelization of human skin because abundant pockets of dermal keratinocytes persist in the outer root sheath of hair and cysts providing competitive foci of re-epithelization. Automated de-epithelization of human and porcine skin xenografts resulted in precise removal of keratinocytes with subcellular precision, providing a smooth live surface where epidermal transplants might engraft with little endogenous competition from residual outer root sheath from rare hairs. CONCLUSIONS: The displacement of the ArF excimer laser devices allows reproducible, smooth, and damage-free ablation of epidermal areas in the animal model.


Assuntos
Epiderme/cirurgia , Lasers de Excimer , Animais , Humanos , Camundongos , Camundongos Nus , Procedimentos Cirúrgicos Minimamente Invasivos , Modelos Animais , Robótica , Transplante de Pele , Suínos , Transplante Heterólogo
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