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1.
Micron ; 141: 102982, 2020 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-33227627

RESUMO

The study aim was to assess the application of atomic force microscopy (AFM) to evaluate erythrocyte morphology in early stages of type 2 diabetes mellitus, and the association with biochemical, anthropometric, diet, and physical activity indicators. This was a pilot cross-sectional study with four groups: healthy individuals, people with prediabetes (PDG), metabolic syndrome (MSG), and diabetes mellitus group (DMG). Blood samples were obtained to assess the erythrocyte morphology and biochemical parameters. Anthropometrical measurements were taken. Besides, a diet and a physical activity questionnaire were applied. The evaluation of the erythrocyte morphology through the AFM showed quantitative and qualitative alterations in the cell's form and size. Compared to the healthy group, the PDG had a reduction in height (-0.80 µm, p < 0.05), and an increase in axial ratio (-0.09 µm, p < 0.05); the MSG had lower concave depth (-0.19 µm, p < 0.05); and the DMG had a decreased height (-0.46 µm, p < 0.05) and concave depth (-0.29 µm, p < 0.05), and higher axial ratio (+0.08 µm) and thickness (+0.32 µm, p < 0.05). The PDG vs. DMG had a statistically significant difference in concave depth (+0.23 µm, p < 0.05) and thickness (-0.26 µm, p < 0.05). The MSG was different than the DMG in variables like axial ratio (-0.05 µm) and thickness (-0.25 µm). Besides, higher values of age, HbA1c, triglycerides, body mass index, waist-to-hip ratio, and physical inactivity were associated with altered erythrocyte morphology. AFM is a promising instrument to assess early but subtle changes in erythrocyte morphology (height, axial ratio, concave depth, thickness) before significant pathological conditions, such as type 2 diabetes mellitus. HbA1c might have a major effect in altered morphology, vs. metabolic parameters like high triglycerides, body mass index, waist, and physical inactivity.

2.
Int. j. odontostomatol. (Print) ; 14(2): 236-241, June 2020. tab
Artigo em Inglês | LILACS | ID: biblio-1090680

RESUMO

There is no information about the possible impact in denture retention after the use of common denture adhesives (DAs) when poor denture foundations (PDF) are present. Moreover, there is a lack of information about which current formulation provides greater retention and for how long. Twelve models from edentulous patients with different ridge shape and border height were used and complete dentures were manufactured. Four different formulation brands of DAs were tested after 10 minutes and three, six, nine, and 12 hours of DA application using a universal testing machine. The Fittydent® and Fixodent® adhesives had the highest retention at 12 hours. The PDF group increased on average its retention by 400 %. However, the group presented lower retention compared to the good denture foundation group. In conclusion, DAs significantly increased denture retention. The PDF group were the most benefited with the application of DAs. The Fixodent® paste had the highest retention.


No existe información acerca del posible impacto en la retención de dentaduras después del uso de adhesivos dentales comunes (DAs) cuando existen rebordes alveolares deficientes (PDF). Más aun, existe una falta de información acerca de cuál formula actual provee mayor retención y por cuanto tiempo. Doce modelos de pacientes edentulos con diferentes formas y alturas en sus rebordes alveolares fueron usados, y dentaduras completas les fueron realizadas. Cuatro diferentes fórmulas y marcas de DAs fueron evaluadas después de 10 minutos, tres, seis, nueve y 12 horas de que se aplicó el DA usando una maquina universal de pruebas. Los adhesivos Fittydent® y Fixodent® presentaron la retención más alta a las 12 horas. El grupo con PDF incrementó su retención hasta en un 400 %. Sin embargo, el grupo presentó menor retención cuando se comparó con el grupo que posee adecuados procesos alveolares. Los DAs incrementaron significativamente la retención de las dentaduras. El grupo PDF fue el más beneficiado con la aplicación de DAs. La pasta Fixodent® provee la más alta retención.


Assuntos
Humanos , Retenção de Dentadura/métodos , Cimentos Dentários/química , Processo Alveolar , Técnicas In Vitro , Adesivos
3.
J Glob Antimicrob Resist ; 21: 285-290, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32315776

RESUMO

OBJECTIVE: This investigation aimed to detect coincidences in the antimicrobial resistance genes (ARG) profiles between members of a group living in a household and to compare them between other groups in order to establish if an exchange of ARG occurs and if dental plaque microbiota can be considered as a source and reservoir of ARG that can be shared between humans and pets. METHODS: One hundred sixty dental plaque samples were obtained from four groups: Shelter dogs group (n=20), adult pet owners and dogs group (AD group, n=40), adult pet owners, children and dogs group (ACD group, n=60), and adult non-pet owners and children group (AC group, n=40). DNA was obtained, and specific primers with polymerase chain reaction for ARG detection were used. RESULTS: The AD group exhibited the most coincidences in their ARG profiles, 14 (70%) of the 20 profiles coincided in 100% followed by the ACD group with 9 (45%) coincidences. While the AC group was the less coincident group, only 7 (35%) of the 20 profiles coincided. tetM was the most prevalent with 53.1%, followed by tetQ with 52.5% and cfxA with 51.2%, while the less prevalent were tetW with 31.8%, blaTEM-1 with 27.5%, and ermC with 18.7%. CONCLUSION: Dental plaque microbiota can be considered as a source and reservoir of ARG that can be shared between humans and dogs living in a household. The dogs seem to play an important role in the transference of ARG, and the children appear to be the most affected by carrying the most significant number of ARG.

4.
J Appl Biomater Funct Mater ; 17(3): 2280800019851771, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31373255

RESUMO

BACKGROUND: The main microorganism associated with the failure of endodontic treatments is Enterococcus faecalis. Although several endodontic therapeutics have demonstrated antimicrobial activity against E. faecalis, the antimicrobial effectiveness of chitosan (CsNPs) and silver nanoparticles (AgNPs) included into conventional endodontic sealers for endodontic therapies is still unclear. AIM: The objective of this study was to evaluate the antibacterial activity increment (AAI) of endodontic sealers containing CsNPs and AgNPs as well as some chemical components against E. faecalis by direct contact assays. METHODS: CsNPs and AgNPs were synthesized by reduction and ionic gelation methods, respectively. Nanoparticles were characterized by dynamic light scattering and energy dispersive X-ray analysis. The bactericidal activity was tested on monolayers on agar plates and collagen membrane surface assays against E. faecalis. RESULTS: The size of CsNPs was 70.6±14.8 nm and zeta potential was 52.0±5.4 mV; the size of AgNPs was 54.2±8.5 nm, and zeta potential was -48.4±6.9 mV. All materials, single or combined, showed an AAI, especially when CsNPs, chlorhexidine (Chx), and the combination of CsNPs-Chx were added. However, the combination of CsNPs-Chx showed the highest (55%) AAI, followed by Chx (35.5%) and CsNPs (11.1%), respectively. There was a significant statistical difference in all comparisons (p < 0.05). Tubliseal (40%) and AH Plus (32%) sealants showed a higher AAI on E. faecalis in the monolayer test and collagen membrane assay analyzed by scanning electron microscopy. CONCLUSIONS: Tubliseal and AH plus sealers combined with nanoparticles, especially CsNPs-Chx, could be used for conventional endodontic treatments in the control of E. faecalis bacteria.


Assuntos
Antibacterianos , Quitosana , Enterococcus faecalis/crescimento & desenvolvimento , Nanopartículas Metálicas/química , Materiais Restauradores do Canal Radicular , Prata , Antibacterianos/química , Antibacterianos/farmacologia , Quitosana/química , Quitosana/farmacologia , Enterococcus faecalis/ultraestrutura , Humanos , Nanopartículas Metálicas/ultraestrutura , Microscopia Eletrônica de Varredura , Materiais Restauradores do Canal Radicular/química , Materiais Restauradores do Canal Radicular/farmacologia , Prata/química , Prata/farmacologia
5.
Spec Care Dentist ; 39(6): 603-609, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31464005

RESUMO

The purpose of this statement is to debate the recommendations of the American Heart Association (AHA) for the prevention of infective endocarditis through an antibiotic prophylaxis protocol and its relation with bacterial resistance to antibiotics. Since dental infections involve biofilms that include several bacterial species (Gram-negative and Gram-positive), it is essential, from the dental point of view, to consider the frequency, magnitude, and duration of bacteremia associated with active dental infections before applying antibiotic prophylaxis. The actual guidelines for antibiotic prophylaxis should be revised according to recent evidence of bacterial resistance. Amoxicillin/clavulanic acid and moxifloxacin should be considered due to their effectiveness against bacteria associated with oral, GU, and GI infections and the low rates of antibiotic resistance associated with these antibiotics, instead of the actual protocol, which includes amoxicillin (2 g) or clindamycin (600 mg) administered an hour before the dental procedures. The breaking point to test the antibiotic bacterial resistance (ABR) had a wide range in the different studies that were analyzed, which could explain the widely varied ABR percentages reported for the various antibiotics used for antibiotic prophylaxis.


Assuntos
Bacteriemia , Endocardite Bacteriana , Endocardite , Antibacterianos , Antibioticoprofilaxia , Humanos , Estados Unidos
6.
Microscopy (Oxf) ; 68(3): 261-270, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-30860262

RESUMO

AIM: To evaluate the erythrocyte morphology in people with prediabetes, T2DM and healthy subjects in a Mexican population and its association with biochemical parameters. METHODS: Cross-sectional study consisted of three groups: healthy (HG), people with prediabetes (PG) and with T2DM (DMG). A blood sample was obtained from all participants to assess the erythrocyte morphology, and levels of HbA1c, glucose and lipid profile. Anthropometrical parameters were also evaluated. RESULTS: It was observed that compared with healthy individuals, people with prediabetes presented a significant decrease in the diameter (-0.08 µm, P = 0.014) and height (-0.07 µm, P = 0.004), as well as people with T2DM (-0.33 µm, P < 0.001 in diameter; and -0.36 µm, P < 0.001 in height). Besides, it was found a significant difference in diameter (-0.25 µm, P < 0.001) and height (-0.29 µm, P < 0.001) between the PG and DMG. No significant differences in the axial ratio between groups. Also, HbA1c, glucose, triglycerides, cholesterol, LDL cholesterol, systolic blood pressure, weight, BMI, waist and hip circumference were significantly associated with diameter and height. CONCLUSIONS: Erythrocyte morphological alterations can serve as an indicator of early diagnosis of T2DM and a factor implicated in the course of the clinical condition, so the correction of these alterations could serve as a treatment for prediabetes and T2DM. It is essential to promote constantly checkups of biochemical and anthropometrical parameters, as well as erythrocyte morphological alterations to prevent the onset of prediabetes and T2DM and possible clinical complications.


Assuntos
Glicemia/análise , Diabetes Mellitus Tipo 2/diagnóstico , Eritrócitos/ultraestrutura , Hemoglobina A Glicada/análise , Lipídeos/sangue , Estado Pré-Diabético/diagnóstico , Adulto , Estudos Transversais , Diabetes Mellitus Tipo 2/patologia , Diagnóstico Precoce , Feminino , Humanos , Masculino , México , Microscopia Eletrônica de Varredura , Pessoa de Meia-Idade , Estado Pré-Diabético/patologia , Inquéritos e Questionários , Adulto Jovem
7.
Medicine (Baltimore) ; 98(11): e14875, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30882692

RESUMO

Dental caries-a highly prevalent public health problem in preschoolers and school children-is the main cause of premature dental loss during childhood, and this may be related to loss of space in the posterior sector. The objective of this study was to evaluate whether interproximal caries and premature tooth loss (D and/or E) are risk factors for loss of space in the posterior sector.A comparative cross-sectional study (split-mouth type) was performed in schoolchildren (6-8 years old). Seventeen gypsum models were evaluated. These children presented with unilateral loss of a dental organ or interproximal caries (teeth D and/or E) and without such affectations on the other side. Measurements were made with a digital Vernier caliper. The dependent variable was the difference (loss of space, mm) between the control and case sides. The independent variables were type of affectation (interproximal caries or tooth loss), sex, age, arcade, and number of interproximal surfaces affected.The mean age was 6.82 ±â€Š0.44 years and 64.7% were boys. The average space loss was 1.09 ±â€Š0.18 mm (control vs case; P < .0001). A greater loss of space was observed among those who lost a dental organ than those with interproximal caries (P = .0119). A correlation was observed between the variable loss of space and the number of interproximal surfaces affected (r = 0.5712, P = .0166).Interproximal caries and tooth loss were risk factors for loss of space in the posterior segment in this sample of Mexican schoolchildren.


Assuntos
Cárie Dentária/complicações , Criança , Estudos Transversais , Cárie Dentária/diagnóstico , Cárie Dentária/epidemiologia , Feminino , Humanos , Masculino , México , Prevalência , Fatores de Risco , Perda de Dente/classificação , Perda de Dente/complicações , Dente Decíduo/fisiopatologia
8.
Med. oral patol. oral cir. bucal (Internet) ; 24(2): e204-e210, mar. 2019. tab
Artigo em Inglês | IBECS | ID: ibc-180644

RESUMO

Background: Neuromuscular impairment makes individuals with cerebral palsy (CP) more prone to drooling. Among the treatment options, there are procedures that interfere with saliva production. It is imperative to evaluate the effect of the different modalities since the reduction in salivary flow rate/production may exacerbate the risk of dental caries. Material and Methods: The aim of this study was to compare the effects of different treatments for drooling on caries risk and salivary parameters in children and adolescents with CP. Study design: A total of 142 children and adolescents with CP, aged 6 to 18 years, were assigned to groups based on the different treatments they had received for drooling: G1-anticholinergic drugs (n = 18), G2-botulinum toxin injection (n = 16), G3-salivary glands surgery (n = 16), G4-no treatment (n = 42), and G5-non-drooling subjects (n = 50). All participants were evaluated on the Simplified Oral Hygiene Index, and for the prevalence of dental caries (decayed, missing, and filled teeth index and white spot lesions). Unstimulated whole saliva was collected, and salivary flow rate and osmolality were measured. Chi-square, ANOVA and Poisson regression were calculated. Prevalence ratios and their respective 95 % confidence intervals were obtained. The significance level was fixed at 5%. Results: No differences were found in the decayed, missing, and filled teeth index (p = 0.128) and Simplified Oral Hygiene Index (p = 0.674) among the different groups. G3 presented significantly higher percentages of WSL (p < 0.001), lower values of salivary flow rate (p < 0.001), and higher values of osmolality (p < 0.001). The white spot lesion prevalence ratio was higher only for G3 (Prevalence ratio = 14.36; IC 95% = 4.64-44.40; p < 0.001). Conclusions: Children and adolescents with CP who had received surgical treatment for drooling exhibited higher number of white spot lesions because of the reduced salivary flow rate and higher salivary osmolality


No disponible


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Paralisia Cerebral/complicações , Cárie Dentária/epidemiologia , Sialorreia/tratamento farmacológico , Antagonistas Colinérgicos/efeitos adversos , Saliva/química , Concentração Osmolar , Suscetibilidade à Cárie Dentária , Estudos Transversais , Toxinas Botulínicas/uso terapêutico , Antagonistas Colinérgicos/uso terapêutico
9.
Int Orthod ; 17(1): 96-102, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30772354

RESUMO

OBJECTIVE: To assess the retention efficiency of three types of temporary zinc oxide cement trademarks on forced eruption using intracranal wire device. METHODS: An in vitro evaluation included intracanal wire device displacement and detachment at 50g load force for 120 days and then the retention resistance at maximum load force. RESULTS: All groups of temporary zinc oxide cements were efficient to support 50g load forces after 120 days. None statistical differences were found between groups. Zinc oxide cements supported a maximum retention load force, which exceeded in more than 84 times the lowest value obtained in controls (420g). CONCLUSION: Zinc oxide cements are efficient to retain intracanal wire devices on forced eruption processes in vitro and allows removal of both when necessary (wire device and cement, respectively).


Assuntos
Cimentos Dentários/química , Extrusão Ortodôntica/métodos , Cimentos de Resina/química , Óxido de Zinco/química , Resinas Acrílicas , Dente Pré-Molar , Colagem Dentária , Materiais Dentários , Cimentos de Ionômeros de Vidro , Humanos , Mandíbula , Teste de Materiais , Fios Ortodônticos , Preparo de Canal Radicular , Resistência à Tração
10.
Korean J Pediatr ; 61(9): 279-284, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30274505

RESUMO

PURPOSE: This study aimed to determine the frequency of abnormalities in the newborn oral cavity and to evaluate the association with prenatal and perinatal factors. METHODS: This cross-sectional study evaluated 2,216 newborns. Oral findings were assessed in the first 24 hours of life using visual examination. Sex, weight, length, gestational age, and medical disorders at birth were recorded. Maternal demographic and medical information was also obtained. RESULTS: The most common oral findings were Bohn's nodules, Epstein's pearls, and dental lamina cysts. Other intraoral findings included odontogenic cysts, ankyloglossia, and natal teeth, among others. In logistic regression analyses, folic acid consumption during pregnancy was significantly associated with Bohn's nodules (odds ratio [OR], 1.79; 95% confidence interval [CI], 1.23-2.55; P=0.002), Epstein's pearls (OR, 1.63; 95% CI, 1.14-2.33; P=0.007), and dental lamina cysts (OR, 1.45; 95% CI, 1.02-2.05; P=0.038). Moreover, preterm births were negatively associated with prevalence of Bohn's nodules (OR, 0.63; 95% CI, 0.50-0.80; P≤0.0001). Comparison between newborns with and without oral inclusion cysts showed that maternal folic acid and iron intake were significantly different (P<0.05). CONCLUSION: Maternal folic acid and iron intake were associated with the prevalence of oral inclusion cysts.

11.
Med. oral patol. oral cir. bucal (Internet) ; 23(2): e211-e215, mar. 2018. graf, tab
Artigo em Inglês | IBECS | ID: ibc-171402

RESUMO

Background: Cerebral palsy (CP) is a permanent neurological disorder accompanied by secondary musculoskeletal masticatory disorder, with repercussion on chewing and deglutition functions. In these conditions, the liquids ingestion is compromised resulting in salivary osmolality alteration. The objective of this study was to compare salivary osmolality, caries experience and caries risk between normoreactive individuals and patients with CP. Material and Methods: The participants were 4-20 years old: 52 patients with CP treated at a reference rehabilitation centre (study group, SG), and 52 normoreactive individuals (control group, CG). Saliva was collected for five minutes using cotton rolls. Following centrifugation, salivary osmolality was determined by freezing point depression osmometry. Evaluations included caries experience (DMFT index), and caries risk based on a caries-risk assessment tool (CAT). Descriptive and inferential statistics (Chi square and Student t tests) were used to compare the groups. Receiver operating characteristic (ROC) analyses were performed and the area under the ROC curve (Az) was calculated. The level of significance was set at 5%. Results: The groups were homogeneous for sex (p=0.843) and age (p=0.128). In the SG, spastic type CP was the most prevalent (80.8%), and patients showed significantly higher salivary osmolality values compared with the CG (p74 for the SG and >54 for the CG in the presence of dental caries. A significant correlation was verified between salivary osmolality and the DMFT index for the SG (p≤0.05). Conclusions: Although patients with CP showed higher salivary osmolality values, higher caries experience and caries risk were not observed compared with normoreactive individuals (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Adolescente , Adulto Jovem , Paralisia Cerebral/complicações , Má Oclusão/complicações , Concentração Osmolar , Cárie Dentária/complicações , Cárie Dentária/prevenção & controle , Curva ROC
12.
Micron ; 105: 11-17, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29145008

RESUMO

Prevalence of type 2 diabetes mellitus (T2DM) has been increasing worldwide. Cardiovascular diseases are one of the main causes of death among people with T2DM. Morphological changes in erythrocytes have been associated with higher risk of cardiovascular diseases. Atomic force microscopy (AFM) is a new technique that allows non-invasive imaging of cells and the evaluation of changes in mechanical properties. AIM: To evaluate by AFM the erythrocytes morphological changes of people with T2DM METHODS: Search was conducted from in PubMed, ScienceDirect, Scielo, and Lilacs. Erythrocyte, type 2 Diabetes Mellitus and, Microscopy, Atomic Force were the keywords used for the search. Papers included were cross-sectional studies performed in humans. RESULTS: Five of seven articles fulfilled the inclusion criteria. Compared with healthy cells, the erythrocytes from individuals affected by T2DM had morphological changes such as a decreased concave depth, diameter, height and a deformation index, while axial ratio, stiffness, adhesive force, aggregation, and rigidity index were increased. The results regarding the erythrocyte roughness were inconclusive. CONCLUSIONS: The AFM is an excellent instrument to study the altered erythrocytes of subjects affected by T2DM. Morphology changes in erythrocytes could lead to cardiovascular events, which are major complications in people living with this disease.


Assuntos
Doenças Cardiovasculares/patologia , Diabetes Mellitus Tipo 2/patologia , Eritrócitos/citologia , Microscopia de Força Atômica/métodos , Doenças Cardiovasculares/sangue , Diabetes Mellitus Tipo 2/sangue , Humanos , Prognóstico , Risco
13.
Acta Odontol Scand ; 76(4): 229-235, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29160117

RESUMO

OBJECTIVE: To determine and identify antibiotic-resistant bacteria (ARB) of oral streptococci from active dental infections in adults and its association with age and gender. MATERIAL AND METHODS: This cross-sectional study included 59 subjects from 18 to 62 years old. Ninety-eighth samples obtained from the subjects were cultivated in agar plates containing antibiotics amoxicillin/clavulanic acid (A-CA), clindamycin, and moxifloxacin (concentrations of 16, 32 or 64 µg/ml). PCR assay was performed to identify bacterial species. RESULTS: The bacterial species that showed more antibiotic-resistance (AR) was S. mutans (45.9%), followed by S. gordonii (21.6%), S. oralis (17.6%), S. sanguinis (9.5%), S. salivarius (5.4%) and S. sobrinus (0%). Moreover, clindamycin (59.4%) showed the highest frequency of AR. Moxifloxacin and A-CA showed an susceptibility >99.1%, while clindamycin showed the lowest efficacy (93.3%); there was a significant statistically difference (p < .01). The age group between 26 and 50 years old (32.2%) and females (28.8%) showed more multiresistance. Clindamycin showed a statistical difference (p < .05) when comparing groups by gender. CONCLUSIONS: Clindamycin was the antibiotic with the highest frequency of ARB and lower bactericidal effect. Moxifloxacin and A-CA showed the highest efficacy and the lowest ARB frequency. Streptococcus mutans was the bacterial specie that showed an increased frequency of AR.


Assuntos
Antibacterianos/administração & dosagem , Farmacorresistência Bacteriana Múltipla , Doenças Dentárias/tratamento farmacológico , Doenças Dentárias/microbiologia , Adulto , Clindamicina/administração & dosagem , Estudos Transversais , Placa Dentária/microbiologia , Feminino , Fluoroquinolonas/administração & dosagem , Humanos , Masculino , Pessoa de Meia-Idade , Moxifloxacina , Streptococcus/isolamento & purificação , Streptococcus mutans/isolamento & purificação
14.
Arch Oral Biol ; 82: 127-133, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28641178

RESUMO

OBJECTIVE: While different virulence factors have been reported of Aggregatibacter actinomycetemcomitans (Aa), there is little information about the stimulatory effect of its DNA. The main purpose of this study was to assess the inflammatory response of human gingival fibroblasts (HGFs) stimulated with A. actinomycetemcomitans DNA. DESIGN: Cytokine levels of IL-6, IL-1α and TNF-α were measured on the supernatant of HGFs activated with 10, 25, 50 and 100µg/ml DNA of Aa during 24h. Primary cultures of HGFs were infected with Aa and its DNA at different times and concentrations to compare its cytotoxic effect. Cell damage and adhesion of Aa to HGFs were evaluated under light microscopy and Scanning electron microscopy respectively. RESULTS: There was a statistical difference (p<0.05) in cytokine expression in HGFs activated by bacterial DNA with a dose dependent on IL-6 expression and a significantly elevated expression of IL-1α and TNF-α compared to Human DNA negative control. Substantial morphological alterations were observed after infection of A. actinomycetemcomitans in HGFs but not with bDNA exposure. Aggregatibacter actinomycetemcomitans showed a high rate of adhesion and cell damage to HGFs after 30min. CONCLUSIONS: Genomic DNA of A. actinomycetemcomitans could be a factor in the pathogenesis of periodontitis that might play a major role in the inflammatory response.


Assuntos
Aggregatibacter actinomycetemcomitans/química , DNA Bacteriano/química , Fibroblastos/metabolismo , Interleucina-1alfa/metabolismo , Interleucina-6/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Aderência Bacteriana , Gengiva/citologia , Humanos , Técnicas In Vitro , Microscopia Eletrônica de Varredura
15.
Acta Odontol Scand ; 75(4): 243-248, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28358286

RESUMO

OBJECTIVE: Historically, it has been shown that rheumatoid arthritis (RA) and periodontitis (PE) share pathophysiological similarities and possibly a genetic background. In order to elucidate the genetic background between both diseases, we evaluated the distributions of five SNPs genotypes and all the possible haplotypes composed in subjects with isolated RA, PE, combined diseases and healthy controls. MATERIALS AND METHODS: The study population consisted of 280 Mexican subjects. Genomic DNA was isolated from buccal epithelial cells collected by cheek scrapings and analyzed for the determination of the following SNPs: IL-1α + 4845 (rs17561), IL-1α -889 (rs1800587), IL-1ß + 3954 (rs1143634), IL-1ß -511(rs16944) and TNF-α -308 (rs1800629). RESULTS: After adjustment for age, sex and smoking status, multiple logistic regression analysis revealed a no significant association in the genotype frequencies of TNF-α -308 and IL-1α + 4845 SNPs. Otherwise a significant association was observed in IL-1ß + 3954 and IL-1ß -511 (p < 0.05) while IL-1α -889 was of borderline statistical significance (p = 0.054). Also, we found three negative associated haplotypes with PE: IL-1α + 4845 G/IL-1ß -511 A, IL-1ß + 3954 C/IL-1ß -511 A and interestingly IL-1α -889 C/IL-1ß -511 A also with a positive association with RA. CONCLUSIONS: Some genotypes and haplotypes are associated with the diseases. But it seems that the genetic background of the association between RA and PE needs to be explored deeper.


Assuntos
Artrite Reumatoide/genética , Citocinas/genética , Grupos Étnicos/genética , Frequência do Gene , Genótipo , Periodontite/genética , Adulto , Alelos , Artrite Reumatoide/complicações , Feminino , Haplótipos , Humanos , Interleucina-1alfa/genética , Interleucina-1beta/genética , Masculino , México , Pessoa de Meia-Idade , Periodontite/complicações , Polimorfismo Genético , Polimorfismo de Nucleotídeo Único/genética , Fator de Necrose Tumoral alfa/genética
16.
Nanomaterials (Basel) ; 6(7)2016 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-28335264

RESUMO

(1) Background: Streptococcus mutans (S. mutans) is the principal pathogen involved in the formation of dental caries. Other systemic diseases have also been associated with specific S. mutans serotypes (c, e, f, and k). Silver nanoparticles (SNP) have been demonstrated to have good antibacterial effects against S. mutans; therefore, limited studies have evaluated the antimicrobial activity of biofunctionalized SNP on S. mutans serotypes. The purpose of this work was to prepare and characterize coated SNP using two different organic components and to evaluate the antimicrobial activity of SNP in clinical isolates of S. mutans strains and serotypes; (2) Methods: SNP with bovine serum albumin (BSA) or chitosan (CS) coatings were prepared and the physical, chemical and microbiological properties of SNP were evaluated; (3) Results: Both types of coated SNP showed antimicrobial activity against S. mutans bacteria and serotypes. Better inhibition was associated with smaller particles and BSA coatings; however, no significant differences were found between the different serotypes, indicating a similar sensitivity to the coated SNP; (4) Conclusion: This study concludes that BSA and CS coated SNP had good antimicrobial activity against S. mutans strains and the four serotypes, and this study suggest the widespread use of SNP as an antimicrobial agent for the inhibition of S. mutans bacteria.

17.
Arch Gynecol Obstet ; 294(1): 47-54, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-26576767

RESUMO

PURPOSE: To identify the association between periodontitis and periodontal pathogens with preterm birth despite the strict control of some important confounders, such as infectious processes and criteria for diagnosis of periodontitis during pregnancy. MATERIALS AND METHODS: In this cross-sectional study were included 70 healthy puerperal women between 20 and 35 years without a history of genitourinary infections during pregnancy. Based on the gestational age they were divided into two groups: 45 with term birth (>37 weeks) and 25 with preterm birth (<37 weeks). Previous informed consent, a gynecologic and dental history that included gynecologic and obstetric background, periodontal status applying different authors' criteria of periodontitis diagnosis, presence of periodontopathogens, dental caries and oral hygiene were recorded. RESULTS: There was no association between periodontitis, periodontopathogens and preterm birth. There were no statistical differences applying different authors' criteria diagnosis of periodontitis. Gingivitis status was similar, but probing depth was greater in preterm birth subjects, perhaps they are young women, and this finding could be an early sign of periodontitis. In like manner, the main periodontal bacterial species are not associated with preterm birth, general hygiene and care habits are poorer than term birth subjects. CONCLUSION: We could suggest that preterm birth is a multifactorial condition and the role of periodontitis and the periodontopathogens itself is not sufficient to trigger the preterm birth. There are factors such as infectious processes and diagnostic criteria for periodontitis that could be responsible for controversial results.


Assuntos
Cárie Dentária/complicações , Gengivite/complicações , Periodontite/complicações , Nascimento Prematuro/epidemiologia , Adulto , Estudos Transversais , Feminino , Idade Gestacional , Humanos , Recém-Nascido , Gravidez , Complicações na Gravidez/epidemiologia , Nascimento a Termo , Adulto Jovem
18.
Rev Invest Clin ; 66(4): 339-44, 2014.
Artigo em Espanhol | MEDLINE | ID: mdl-25695299

RESUMO

OBJECTIVE: To evaluate through functional teeth index, the impact in schoolchildren in communities with and without dental community support. MATERIAL AND METHODS: A cross-sectional study was carried out in communities with dental care provided by dental clinics of Sinaloa State University. The study sample was 2,083 schoolchildren. We used the criteria proposed by WHO for the index of decayed, missing-teeth (DMFT) for permanent dentition, index for prima- ry teeth (dmft) and the rate of functional teeth (RFT). For bivariate analysis, the nonparametric tests Pearson f and Mann-Whitney U were used. RESULTS: The comparisons of DMFT (0.20) and RFT (13.95) of children with and DMFT (0.43) and RFT (14.82) of children without community dental support showed statistical significant difference (p < 0.05). There was a statistically significant difference in DMFT and RFT between samples with and without community dental support (p < 0.05). The age showed a statistically significant difference between the DMFT and dmft (p < 0.05). Primary dentition required more dental care than permanent dentition. There was access for dental treatment, but it is not used for the population. CONCLUSIONS: The RFT provided precise information than DMFT, mainly due to register the changes of actions focused to recover the teeth function in the oral cavity.


Assuntos
Assistência Odontológica para Crianças/estatística & dados numéricos , Cárie Dentária/epidemiologia , Serviços de Saúde Bucal/provisão & distribução , Criança , Estudos Transversais , Índice CPO , Dentição Permanente , Feminino , Acesso aos Serviços de Saúde , Humanos , Masculino , México/epidemiologia , Estatísticas não Paramétricas , Dente Decíduo
19.
Rev Invest Clin ; 65(1): 24-9, 2013.
Artigo em Espanhol | MEDLINE | ID: mdl-23745441

RESUMO

OBJECTIVE: To analyze the caries risk through the Bratthall's Cariogram (BC) and the frequency of dental caries in a Mexican northwest children population for 12 months period. MATERIAL AND METHODS: A cohort of 583 scholar children between 6 and 10 years old from Sinaloa state was involved for 12 months period (2007-2008). The Bratthall's Cariogram was used to predict caries risk and the. WHO's criteria were used to obtain the caries index. The caries risk association with clinical variables was analyzed by logistic regression analysis and Sperman's Rho rank correlation test was used to evaluate the correlation between DMFT index and BC. RESULTS: The caries risk increased with respect to age (p < 0.05), the CB identified correctly children for high risk (85%) and low risk (65%) caries for a 12 months period. The baseline values of BC showed a positive correlation with DMFT index (0.86 and p = 0.0001); the diagnostic test evaluation showed the following values: positive predictive value of 87%, negative predictive value of 63%, sensitivity of 93% and specificity of 63%. CONCLUSIONS: The caries risk increased with the age in the studied population. The Bratthall's Cariogram is a useful screening test to evaluate the risk for dental caries at individual and population levels.


Assuntos
Cárie Dentária/epidemiologia , Fatores Etários , Criança , Estudos de Coortes , Índice CPO , Feminino , Humanos , Incidência , Modelos Logísticos , Masculino , Programas de Rastreamento , México/epidemiologia , Valor Preditivo dos Testes , Risco , Medição de Risco , Sensibilidade e Especificidade
20.
Dent Mater J ; 31(6): 1021-7, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23207210

RESUMO

The aim of this study was to evaluate the bond strength of three adhesive systems: Excite™, Adper Prompt L-Pop™ and AdheSE One™ to varying degrees of fluorotic enamel using micro-tensile bond strength (µTBS) tests. Human enamel was classified according to the Thylstrup and Fejerskov Index. The interface resin-enamel was observed using stereoscopic and electron microscopy. The Excite™, achieved the highest µTBS when bonded to healthy enamel and decreased as the degree of fluorosis increased (p<0.05). The Prompt L-Pop™ improved the bonding on moderate and severe fluorosis. The µTBS of the AdheSE One™, was significantly lower in all degrees of fluorotic enamel (p<0.05) indicating a very poor bonding ability to enamel. These results will provide clinicians with preliminary data to assist them in the selection of the most effective adhesive systems for treatment of fluorosis enamel, resulting in more successful restorative care.


Assuntos
Condicionamento Ácido do Dente/métodos , Resinas Compostas , Colagem Dentária , Esmalte Dentário , Fluorose Dentária/patologia , Cimentos de Resina , Resinas Acrílicas , Adulto , Esmalte Dentário/patologia , Análise do Estresse Dentário , Humanos , Teste de Materiais , Metacrilatos , Microscopia Eletrônica de Varredura , Resistência à Tração , Adulto Jovem
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