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1.
Zhongguo Dang Dai Er Ke Za Zhi ; 22(1): 53-57, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31948525

RESUMO

OBJECTIVE: To study the changes in the serum levels of Chemerin and Omentin-1 in children with Kawasaki disease (KD) in the acute stage after intravenous immunoglobulin (IVIG) treatment and related clinical significance. METHODS: A total of 60 children who were diagnosed with KD from January 2015 to April 2019 were enrolled as subjects. Forty healthy children and 40 children with acute infectious diseases were enrolled as the healthy control group and the infection control group respectively. According to the sensitivity to IVIG treatment, the children with KD were divided into an IVIG sensitive group with 51 children and a non-IVIG sensitive group with 9 children. According to the presence or absence of coronary artery lesion, the children with KD were divided into a CAL group with 13 children and a non-CAL group with 47 children. ELISA was used to measure the serum levels of Omentin-1 and Chemerin before and after the treatment. RESULTS: The children with KD had significantly higher serum levels of Chemerin and Omentin-1 than the healthy control and infection control groups before treatment (P<0.05). After 48 hours of treatment, the IVIG sensitive group had a significant reduction in the serum level of Chemerin (P<0.05), while there was no significant change in the serum level of Omentin-1 after treatment (P>0.05). Before treatment, the non-IVIG sensitive group had a significantly higher serum level of Chemerin than the IVIG sensitive group (P<0.05), and the CAL group had a significantly higher serum level of Chemerin than the non-CAL group, while there was no significant difference in the serum level of Omentin-1 between the IVIG sensitive and non-IVIG sensitive groups, as well as between the CAL and non-CAL groups (P>0.05). CONCLUSIONS: High serum levels of Chemerin and Omentin-1 may play an important role in the development and progression of KD. Chemerin may be involved in the development of CAL in children with KD. The serum level of Chemerin may be used as a new index for predicting the sensitivity to IVIG treatment.


Assuntos
Síndrome de Linfonodos Mucocutâneos , Adipocinas , Quimiocinas , Criança , Doença da Artéria Coronariana , Humanos , Imunoglobulinas Intravenosas
2.
Mol Med Rep ; 21(1): 429-437, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31746387

RESUMO

The present study examined whether lipoxin A4 (LXA4) increases the expression of HO­1, and inhibits the production of interleukin 6 (IL­6) and monocyte chemotactic protein 1 (MCP­1) in LXA4­induced protection during hyperoxia­induced injury in murine lung epithelial cells (MLE­12) and what signal pathway may participate in the actions of LXA4 inhibiting IL­6 and MCP­1. MLE­12 cells were exposed to air or hyperoxia with or without pretreatment with LXA4, Zinc protoporphyrin IX (ZnPP­IX), IL­6, anti­IL­6, MCP­1, anti­MCP­1, inhibitors of p38 mitogen­activated protein kinase (p38 MAPK), protein kinase B (Akt) and extracellular signal­regulated kinase 1/2 (ERK1/2) signaling pathways. The cell survival rates, cell viability, apoptosis rates, expression of superoxide dismutase (SOD), heme oxygenase­1 (HO­1), IL­6 and MCP­1, and the activations of p38 MAPK, ERK1/2 and Akt were measured. LXA4 significantly increased the cell survival rates, cell viability, SOD levels and HO­1 expression, reduced the apoptosis rates, and inhibited the MCP­1 and IL­6 levels induced by hyperoxia in cells. ZnPP­IX, an inhibitor of HO­1, blocked LXA4­induced protection on cell viability in cells exposed to hyperoxia. Anti­IL­6 and anti­MCP­1 improved the cell viability of cells exposed to hyperoxia. Inhibition of p38 MAPK and ERK1/2 blocked the expression of MCP­1 and IL­6 induced by hyperoxia. LXA4 inhibited the activation of p38 MAPK and ERK1/2 induced by hyperoxia, and increased the activation of the Akt signaling pathway, which was inhibited by hyperoxia. Therefore, LXA4 attenuated hyperoxia­induced injury in MLE­12 cells via the upregulation of HO­1 expression. The protection of LXA4 in hyperoxia­induced cell injury may be associated with the downregulation IL­6 and MCP­1 levels via the inhibition of the p38 MAPK and ERK1/2 signaling pathways.

3.
Zhongguo Dang Dai Er Ke Za Zhi ; 21(7): 701-707, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31315772

RESUMO

OBJECTIVE: To study the structural features of intestinal flora in preterm rats with cognitive impairment and the association of the change in intestinal flora with cognitive impairment in preterm rats. METHODS: Sprague-Dawley rats at 16-17 days of gestation were intraperitoneally injected with lipopolysaccharide for two consecutive days to establish a model of cognitive impairment, and the rats treated with intraperitoneally injected phosphate-buffered saline were established as the control group. Cesarean section was performed on day 21 of gestation, and preterm rats were randomly assigned to healthy maternal rats for feeding. The place navigation test in the Morris water maze was used to evaluate cognition on day 30 after birth. According to the result, the preterm rats were divided into cognitive impairment group with 21 rats and normal control group with 10 rats. Hematoxylin and eosin staining was used to observe pathological changes of the hippocampus, and fecal samples were collected for 16S rRNA sequencing and analysis. A principal component analysis (PCA) was performed for intestinal flora. RESULTS: Compared with the normal control group, the cognitive impairment group showed degeneration and necrosis of a large number of neurons in the hippocampus. Compared with the normal control group, the cognitive impairment group had significant reductions in the abundance and diversity of intestinal flora (P<0.05), with a significant increase in the abundance of Proteobacteria at the phylum level (P<0.05), as well as significant reductions in the abundance of Prevotella and Lactobacillus and significant increases in the abundance of Staphylococcaceae and Oligella at the order, family, and genus levels (P<0.05). PCA showed a significant difference in the composition of intestinal flora between the two groups. CONCLUSIONS: There is a significant change in the structure of intestinal flora in preterm rats with cognitive impairment, which provides a basis for the treatment and intervention of microecological changes due to cognitive impairment after preterm birth.


Assuntos
Disfunção Cognitiva , Microbioma Gastrointestinal , Animais , Cesárea , Feminino , Gravidez , RNA Ribossômico 16S , Ratos , Ratos Sprague-Dawley
4.
Int J Mol Med ; 43(1): 371-381, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30387808

RESUMO

Post­translational modification via small ubiquitin­like modifier (SUMO) is involved in the regulation of various important cellular processes. SUMO modification can be regulated at the level of conjugation, and can also be reversed by the SUMO­specific proteases (SENPs). However, current studies of the regulation and function of SENP in lung development remain limited. In this study, the expression levels of SENP1 and SUMO1 were assessed during lung development in rats. SUMO1 modification occurred during lung development and changes in SENP1 expression were consistent with the changes in the presence of free SUMO1. In order to investigate the function of SENP1, alveolar type (AT) 2 cells were transfected with SENP1­targeting small interfering RNA, and the proliferation, apoptosis and differentiation function of AT2 cells was subsequently evaluated. Marked upregulation of conjugated SUMO1 was observed following SENP1 inhibition. Furthermore, depletion of SENP1 resulted in increased apoptosis, decreased proliferation and impaired differentiation status of AT2 cells. Thus, the results support that SENP1 is an essential regulator of the balance between SUMOylation and deSUMOylation during lung development, specifically affecting the proliferation and differentiation status of AT2 cells.


Assuntos
Células Epiteliais Alveolares/citologia , Células Epiteliais Alveolares/metabolismo , Diferenciação Celular , Cisteína Endopeptidases/fisiologia , Organogênese , Animais , Apoptose/efeitos dos fármacos , Apoptose/genética , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Cisteína Endopeptidases/genética , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Humanos , Organogênese/efeitos dos fármacos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos Sprague-Dawley , Proteína SUMO-1/genética , Proteína SUMO-1/metabolismo , Tretinoína/farmacologia , Regulação para Cima/efeitos dos fármacos , Regulação para Cima/genética
5.
Dalton Trans ; 47(38): 13466-13471, 2018 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-30183795

RESUMO

Three new transition metal oxychlorides MBi(SeO3)2(H2O)Cl (M = Co, Ni, Cu) have been firstly synthesized through a hydrothermal reaction method at 200 °C. They were structurally determined to be isostructural with the Pbca space group of the orthorhombic system. They feature a 3D framework topology with two-dimensional tunnels intersected and filled with Cl anions parallel to the crystallographic bc-plane. Two neighboring MO5Cl octahedra are connected by a sharing edge into a M2O8Cl2 dimer which serves as the structural knots to knit the 2D [Bi(SeO3)2]∞ layers in the ab-plane together into the total 3D crystal architecture. The optical band gaps of CoBi(SeO3)2(H2O)Cl (1), NiBi(SeO3)2(H2O)Cl (2) and CuBi(SeO3)2(H2O)Cl (3) were evaluated to be 3.7 eV, 3.5 eV and 3.2 eV, respectively, through extrapolating the UV-vis-NIR optical absorption spectra. Besides, the spin-allowed d-d transition absorption spectra of the transition ion centers are observed in three compounds exhibiting different colors. Compounds 1, 2 and 3 can stay thermally stable below 370 °C, 400 °C and 300 °C, respectively, as found from the results of thermal analysis based on the simultaneous thermogravimetry and differential scanning calorimetry techniques. 1, 2 and 3 exhibit antiferromagnetic properties below Néel temperatures 6 K, 18 K and 52 K, respectively. Above the Néel temperatures, Curie-Weiss behavior dominates in the M-T process for the three compounds. The cell parameters are listed: a = 14.056 Å, b = 7.582 Å, c = 14.996 Å, and Z = 8 for 1, a = 14.083 Å, b = 7.575 Å, c = 14.860 Å, and Z = 8 for 2, and a = 14.576 Å, b = 7.371 Å, c = 14.656 Å, and Z = 8 for 3.

6.
AIDS Care ; 30(10): 1228-1230, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-29902931

RESUMO

A large proportion of people who are HIV positive do not know their serostatus because facility-based provider-initiated HIV testing and counseling, and voluntary counseling and testing, have not been efficiently implemented in China. Therefore, a new HIV testing strategy must be developed to improve testing services so that more HIV infections can be detected earlier. In this study, we established an anonymous internet-aided urine-based HIV testing service for men who have sex with men (MSM) from 1 April 2016 to 20 January 2017. In total, 3092 urine sample collection packs were distributed by grassroots organizations to MSM; 1977 (69.3%) packs were mailed back to the laboratory; and 1911 (96.7%) eligible samples were tested for HIV antibody. The rate of HIV antibody positivity was 7.1% (135/1901), excluding 10 previously-identified HIV infections. Of those tested, 65.4% (1243/1901) participants obtained their results from our website, 94 (69.6%) of 135 newly-identified urine HIV antibody-positive participants were contacted by CDC staff, and 61.7% (58/94) reported undergoing blood HIV antibody confirmation testing after learning of their urine HIV antibody test results. Of those who were tested for venous HIV antibody, 84.5% (49/58) reported being confirmed HIV antibody positive. Thirty-six of the newly diagnosed participants were successfully referred to a hospital to receive antiretroviral therapy. The rate of confirmed HIV antibody positivity was estimated to be 72.8-89.2 times of that of routine HIV antibody testing. In conclusion, this approach offers an alternative efficient HIV testing strategy to identify HIV positive persons in vulnerable populations.


Assuntos
Testes Anônimos , Anticorpos Anti-HIV/urina , Infecções por HIV/diagnóstico , Homossexualidade Masculina/psicologia , Internet , Adulto , China , Aconselhamento , Estudos de Viabilidade , Humanos , Masculino , Coleta de Urina
7.
Zhongguo Dang Dai Er Ke Za Zhi ; 20(5): 403-409, 2018 May.
Artigo em Chinês | MEDLINE | ID: mdl-29764579

RESUMO

OBJECTIVE: To study the expression of SUMO-modified CCAAT enhancer binding protein α (C/EBPα) in preterm rat model of bronchopulmonary dysplasisa (BPD) induced by hyperoxia exposure and its role. METHODS: Eighteen preterm rats were randomly divided into an air group and a hyperoxia group (n=9 each). The model of BPD was prepared in preterm rats exposed to hyperoxia. The rats from the two groups were sacrificed on postnatal days 4, 7 and 14 respectively (3 rats at each time) and lung tissues were harvested. Periodic acid-Schiff (PAS) staining was used to observe the differentiation of rat lung tissues. Ki67 expression was detected by immunohistochemistry. Western blot was used to measure the protein expression of small ubiquitin-related modifier-1(SUMO1) and C/EBPα. A co-immunoprecipitation assay was performed to measure the protein expression of SUMO-modified C/EBPα. RESULTS: Compared with the air group, the hyperoxia group showed a decreased glycogen content in the lung tissue on postnatal day 4, and an increased content on postnatal days 7 and 14. Over the time of hyperoxia exposure, the hyperoxia group showed an increased expression of Ki67 in the lung tissue compared with the air group at all time points. Compared with the air group, the protein expression of C/EBPα increased on postnatal day 4 and decreased on postnatal days 7 and 14 in the hyperoxia group (P<0.05). The hyperoxia group had significantly upregulated expression of SUMO1 and SUMO-modified C/EBPα compared with the air group at all time points (P<0.05). In the hyperoxia group, the protein expression of SUMO-modified C/EBPα was positively correlated with the glycogen content (r=0.529, P<0.05) and the expression of Ki67 (r=0.671, P<0.05). CONCLUSIONS: Hyperoxia may induce over-proliferation and differentiation disorders of alveolar epithelial cells in preterm rat model of BPD, possibly through an increased expression of SUMO-modified C/EBP&alpha.


Assuntos
Displasia Broncopulmonar/etiologia , Proteína alfa Estimuladora de Ligação a CCAAT/metabolismo , Hiperóxia/patologia , Sumoilação , Animais , Animais Recém-Nascidos , Displasia Broncopulmonar/metabolismo , Displasia Broncopulmonar/patologia , Proliferação de Células , Modelos Animais de Doenças , Hiperóxia/complicações , Antígeno Ki-67/análise , Alvéolos Pulmonares/patologia , Ratos , Ratos Sprague-Dawley
8.
J Sci Food Agric ; 98(12): 4395-4402, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29603275

RESUMO

Aroma significantly contributes to flavor, which directly affects the commercial quality of strawberries. The strawberry aroma is complex as many kinds of volatile compounds are found in strawberries. In this review, we describe the current knowledge of the constituents and of the biosynthesis of strawberry volatile compounds, and the effect of postharvest treatments on aroma profiles. The characteristic strawberry volatile compounds consist of furanones, such as 2,5-dimethyl-4-hydroxy-3(2H)-furanone and 4-methoxy-2,5-dimethyl-3(2H)-furanone; esters, including ethyl butanoate, ethyl hexanoate, methyl butanoate, and methyl hexanoate; sulfur compounds such as methanethiol, and terpenoids including linalool and nerolidol. As for postharvest treatment, the present review discusses the overview of aroma volatiles in response to temperature, atmosphere, and exogenous hormones, as well as other treatments including ozone, edible coating, and ultraviolet radiation. The future prospects for strawberry volatile biosynthesis and metabolism are also presented. © 2018 Society of Chemical Industry.


Assuntos
Fragaria/química , Compostos Orgânicos Voláteis/química , Aromatizantes/química , Frutas/química , Odorantes/análise
9.
Adv Mater ; 30(21): e1706317, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29611231

RESUMO

Incorporation of N,S-codoped nanotube-like carbon (N,S-NTC) can endow electrode materials with superior electrochemical properties owing to the unique nanoarchitecture and improved kinetics. Herein, α-MnS nanoparticles (NPs) are in situ encapsulated into N,S-NTC, preparing an advanced anode material (α-MnS@N,S-NTC) for lithium-ion/sodium-ion batteries (LIBs/SIBs). It is for the first time revealed that electrochemical α → ß phase transition of MnS NPs during the 1st cycle effectively promotes Li-storage properties, which is deduced by the studies of ex situ X-ray diffraction/high-resolution transmission electron microscopy and electrode kinetics. As a result, the optimized α-MnS@N,S-NTC electrode delivers a high Li-storage capacity (1415 mA h g-1 at 50 mA g-1 ), excellent rate capability (430 mA h g-1 at 10 A g-1 ), and long-term cycling stability (no obvious capacity decay over 5000 cycles at 1 A g-1 ) with retained morphology. In addition, the N,S-NTC-based encapsulation plays the key roles on enhancing the electrochemical properties due to its high conductivity and unique 1D nanoarchitecture with excellent protective effects to active MnS NPs. Furthermore, α-MnS@N,S-NTC also delivers high Na-storage capacity (536 mA h g-1 at 50 mA g-1 ) without the occurrence of such α → ß phase transition and excellent full-cell performances as coupling with commercial LiFePO4 and LiNi0.6 Co0.2 Mn0.2 O2 cathodes in LIBs as well as Na3 V2 (PO4 )2 O2 F cathode in SIBs.

10.
Int J Mol Med ; 40(4): 1037-1046, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28902364

RESUMO

CCAAT enhancer binding protein alpha (C/EBPα) is a transcription factor regulating the core aspects of cell growth and differentiation. The present study investigated the level and functional role of C/EBPα during the development of the rat lung. C/EBPα protein exhibits a dynamic expression pattern. The correlation between the expression of C/EBPα protein and the content of glycogen during lung maturation was analyzed to understand the function of C/EBPα in lung differentiation. The high expression of C/EBPα coincides with the reduction of glycogen in the fetal lung. In addition, the authors identified that changes in the level of C/EBPα are associated with the secretion of pulmonary surfactant. C/EBPα is modified by small ubiquitin-related modifier (SUMO) post-translationally. The results of double immunofluorescence staining and immunoprecipitation demonstrated that SUMO-modified C/EBPα was present in the lung. The sumoylated C/EBPα gradually decreased during lung differentiation and was negatively correlated with pulmonary surfactant secretion, thereby suggesting that the SUMO modification may participate in C/EBPα-mediated lung growth and differentiation. These results indicated that C/EBPα played a role in lung development and provided the insight into the mechanism underlying SUMO-modification.


Assuntos
Proteína alfa Estimuladora de Ligação a CCAAT/metabolismo , Pulmão/embriologia , Pulmão/metabolismo , Proteínas Associadas a Surfactantes Pulmonares/metabolismo , Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina/metabolismo , Animais , Diferenciação Celular , Feminino , Glicogênio/metabolismo , Pulmão/citologia , Morfogênese , Fosfatidilcolinas/metabolismo , Fosfolipídeos/metabolismo , Ratos Sprague-Dawley
11.
Inflammation ; 40(6): 2094-2108, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28819748

RESUMO

Transforming growth factor-ß (TGF-ß) superfamily members are key regulators for lung development and progress of bronchopulmonary dysplasia (BPD). The mechanisms by which lipoxin A4 (LXA4) attenuates development of BPD have not been clarified. Neonatal murine BPD models were inducted by hyperoxia treatment. Neonatal mice were exposed to room air or 85% O2 hyperoxia with or without treatment with 5S,6R-methyl-LXA4 or anti-TGF-ß antibodies. Mouse lung epithelial cells (MLE-12 cells) and mouse embryonic fibroblasts (NIH/3T3 cells) were cultured in room air or 85% O2 followed by treatment of LXA4, anti-TGF-ß antibodies, and let-7c mimic/anti-microRNA transfections. Treatment with 5S,6R-methyl-LXA4 and anti-TGF-ß antibodies both attenuated the mice alveolar simplification induced by hyperoxia. Hyperoxia treatment significantly altered pulmonary basal mRNA and protein expressions of several important extracellular matrix (ECM) and ECM remodeling proteins including fibronectin, α-smooth muscle actin (α-SMA), tissue inhibitor of metalloproteinase-1 (TIMP-1), elastin, tenascin C, collagen I, and matrix metalloproteinase-1 (MMP-1). 5S,6R-methyl-LXA4 and anti-TGF-ß antibodies suppressed the mRNA and protein expressions of TGF-ß1 and TGF-ßR1 but not TGF-ßR2 in the lungs exposed to hyperoxia. Treatment with LXA4 and anti-TGF-ß antibodies alleviated hyperoxia-induced injury of the NIH/3T3 cells identified by morphologic observation and flow cytometry, and expressions of ECM, ECM remodeling proteins, and TGF-ß1 signaling pathway, but reversed by transfection with let-7c anti-miRNA. LXA4 upregulated the let-7c expression in MLE-12 cells, transfection with let-7c anti-miRNA, inhibited the LXA4-induced let-7c expression in MLE-12 cells exposed to hyperoxia and reduced the relative luciferase activity of let-7c binding with let-7c binding sites of the TGF-ßR1 3' UTR. Treatment with 5S,6R-methyl-LXA4 and anti-TGF-ß antibodies significantly improved histology, ECM, and ECM remodeling proteins in the lungs isolated from the murine BPD model induced by hyperoxia. The LXA4-imparted protective effects on hyperoxia-induced lung injury are mediated by upregulation of let-7c and inhibition of TGF-ß1 and subsequent downregulation of TGF-ß1 signaling pathway.


Assuntos
Displasia Broncopulmonar/prevenção & controle , Lipoxinas/farmacologia , MicroRNAs/metabolismo , Transdução de Sinais/efeitos dos fármacos , Fator de Crescimento Transformador beta1/metabolismo , Animais , Animais Recém-Nascidos , Anti-Inflamatórios não Esteroides/farmacologia , Displasia Broncopulmonar/tratamento farmacológico , Regulação para Baixo/efeitos dos fármacos , Camundongos , Células NIH 3T3 , Regulação para Cima
12.
Mol Med Rep ; 16(2): 1493-1501, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28586043

RESUMO

Hyperoxia is one of the primary causes of bronchopulmonary dysplasia, which may occur in premature infants following supplemental oxygen therapy. Glucose regulated protein 78 (GRP78), which is a molecular chaperone located in the lumen of the endoplasmic reticulum (ER), has been reported to regulate hyperoxia­associated ER stress. The role of GRP78 in lung epithelial cells during hyperoxia remains to be elucidated. In the present study, the A549 cultured human lung epithelial cell line was exposed to hyperoxic conditions, and then transfected with short interfering (si)RNA targeted to GRP78. siRNA or pEGFP­N1 plasmid were used to knockdown or overexpress specific genes, reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to detect RNA and protein levels of gene expression, and flow cytometry was used to detect apoptosis. The expression levels of ER stress­associated genes were determined, and a significant increase in C/EBP homologous protein (CHOP) expression and apoptosis of A549 cells was observed, following GRP78 knockdown. The overexpression of CHOP downregulated B­cell lymphoma (Bcl)­2 expression levels, upregulated BCL2 associated X (Bax), and increased apoptosis of A549 cells under conditions of hyperoxia. CHOP knockdown demonstrated the opposite effect on Bcl­2 and Bax expression levels. These results suggested that GRP78 silencing promoted lung epithelial cell apoptosis during hyperoxia, via regulation of the CHOP pathway.


Assuntos
Células Epiteliais Alveolares/patologia , Apoptose , Inativação Gênica , Proteínas de Choque Térmico/metabolismo , Hiperóxia/metabolismo , Hiperóxia/patologia , Transdução de Sinais , Fator de Transcrição CHOP/metabolismo , Células A549 , Fator 6 Ativador da Transcrição/metabolismo , Células Epiteliais Alveolares/metabolismo , Endorribonucleases/metabolismo , Humanos , Proteínas Serina-Treonina Quinases/metabolismo , RNA Interferente Pequeno/metabolismo , Proteína X Associada a bcl-2/metabolismo , eIF-2 Quinase/metabolismo
13.
ACS Appl Mater Interfaces ; 9(14): 12518-12527, 2017 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-28345854

RESUMO

In this work, a flexible and self-supporting P-doped carbon cloth (FPCC), which is composed of interwoven mesh of hollow microtubules with porous carbon walls, is prepared via a vacuum-sealed doping technology by employing the commercially available cotton cloth as sustainable and scalable raw material. When directly used as binder-free anode for sodium-ion batteries, the as-prepared FPCC delivers superior Na-storage properties in terms of specific capacity up to 242.4 mA h g-1, high initial Coulombic efficiency of ∼72%, excellent rate capabilities (e.g., 123.1 mA h g-1 at a high current of 1 A g-1), and long-term cycle life (e.g., ∼88% capacity retention after even 600 cycles). All these electrochemical data are better than the undoped carbon cloth control, demonstrating the significance of P-doping to enhance the Na-storage properties of cotton-derived carbon anode. Furthermore, the technologies of electrochemical impedance spectroscopy and galvanostatic intermittent titration technique are implemented to disclose the decrease of charge transfer resistance and improvement of Na-migration kinetics, respectively.

14.
World J Pediatr ; 13(3): 228-235, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27995540

RESUMO

BACKGROUND: High-mobility group box-1 (HMGB1) protein acts as an important pro-infl ammatory mediator, which is capable of activating inflammation and tissue repair. HMGB1 can bind to its receptor such as advanced glycation end products (RAGE). RAGE, in turn, can promote the production of pro-inflammatory cytokines. Soluble RAGE (sRAGE) is a truncated form of the receptor comprising the extracellular domain of RAGE and can inhibit RAGE-activation. The objective of this study was to investigate whether HMGB1 and RAGE are involved in the development of brain injury in preterm infants. METHODS: In total, 108 infants ≤34 weeks gestation at birth were divided into 3 groups according to cranial altrasound scan: mild brain damage (n=33), severe brain damage (n=8) and no brain damage (n=67). All the placentas were submitted for pathologic evaluation. Histological chorioamnionitis (HCA) was defined as neutrophil infi ltration of amniotic membranes, umbilical cord or chorionic plate. Expressions of HMGB1 and RAGE proteins were assessed by immunohistochemical analysis. The concentration of HMGB1 and sRAGE in umbilical cord blood were measured by enzyme-linked immunosorbent assay. RESULTS: The frequency of HCA was 30.12%. HCA was associated with elevated concentrations of HMGB1 and decreased sRAGE in umbilical cord blood. The severe brain injury group demonstrated higher cord blood HMGB1 concentrations (P<0.001) and lower sRAGE concentrations (P<0.001) than both other groups. Brain injury in the premature infants was linked to intense staining for HMGB1/RAGE, particularly in infl ammatory cells. CONCLUSIONS: Changes of cord blood HMGB1 and sRAGE of premature infants had direct relationship with the degree of infl ammation and severity of brain damage. Monitoring sRAGE and HMGB1 levels may be helpful to predict intrauterine infection and brain injury in premature infants.


Assuntos
Lesões Encefálicas/congênito , Lesões Encefálicas/metabolismo , Produtos Finais de Glicação Avançada/metabolismo , Proteína HMGB1/metabolismo , Recém-Nascido Prematuro , Biomarcadores/metabolismo , Lesões Encefálicas/diagnóstico por imagem , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imuno-Histoquímica , Recém-Nascido , Masculino , Ultrassonografia
15.
AIDS Care ; 29(5): 644-653, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-27832699

RESUMO

Global literature revealed that seropositive men who have sex with men (MSM) posed an even higher risk compared to their seronegative counterparts. Identifying risk factors that contribute to HIV-risk behaviors will help to curb the rapid HIV transmission among this group. Our hypothesis was that MSM with substance use were more likely to conduct HIV-risk behaviors, even after accounting for repeated measures. In the current study, we employed a cohort study design by following a group of 367 HIV-positive MSM up to four visits for one year to collect information regarding their sexual behaviors and history of substance use in the past three months. We used Generalized Estimating Equations (GEE) models to account both within- and between-subject variation when assessing associations between substance use and HIV-risk behaviors. A total of 367 MSM were included at the baseline with a mean age of 29.6 years. After accounting for potential confounders and time-varying effects, our models indicated that drug and alcohol use increase HIV risks at the population level by increasing risks of drinking alcohol before sex, having unprotected sex with men and seropositive partners, having more lifetime female sex partners and having a higher number of male sexual partners in the past three months. The current study is one of the first studies with repeated measures to evaluate the association between substance use and sexual risk behaviors among MSM in China. Findings in the current study have several implications for future research. We call for more rigorous study design for future research to better capture changes of risky behaviors among this at-risk population.


Assuntos
Soropositividade para HIV/psicologia , Assunção de Riscos , Transtornos Relacionados ao Uso de Substâncias/psicologia , Sexo sem Proteção , Adulto , Consumo de Bebidas Alcoólicas/psicologia , China , Estudos de Coortes , Feminino , Soropositividade para HIV/transmissão , Homossexualidade Masculina , Humanos , Masculino , Projetos de Pesquisa , Fatores de Risco , Parceiros Sexuais , Inquéritos e Questionários , Adulto Jovem
16.
Zhongguo Dang Dai Er Ke Za Zhi ; 18(11): 1069-1074, 2016 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-27817767

RESUMO

OBJECTIVE: To compare the therapeutic effects of high-frequency oscillatory ventilation+pulmonary surfactant (HFOV+PS), conventional mechanical ventilation+pulmonary surfactant (CMV+PS), and conventional mechanical ventilation (CMV) alone for acute lung injury/acute respiratory distress syndrome (ALI/ARDS) in neonates. METHODS: A total of 136 neonates with ALI/ARDS were enrolled, among whom 73 had ALI and 63 had ARDS. They were divided into HFOV+PS group (n=45), CMV+PS group (n=53), and CMV group (n=38). The neonates in the first two groups were given PS at a dose of 70-100 mg/kg. The partial pressure of oxygen (PaO2), partial pressure of carbon dioxide (PaCO2), PaO2/fraction of inspired oxygen (FiO2), oxygenation index (OI), and respiratory index (RI) were measured at 0, 12, 24, 48, and 72 hours of mechanical ventilation. RESULTS: At 12, 24, and 48 hours of mechanical ventilation, the HFOV+PS group had higher PaO2 and lower PaCO2 than the CMV+PS and CMV groups (P<0.05). At 12, 24, 48, and 72 hours of mechanical ventilation, the HFOV+PS group had higher PaO2/FiO2 and lower OI and RI than the CMV+PS and CMV groups (P<0.05). The HFOV+PS group had shorter durations of mechanical ventilation and oxygen use than the CMV+PS and CMV groups (P<0.05). There were no significant differences in the incidence rates of air leakage and intracranial hemorrhage and cure rate between the three groups. CONCLUSIONS: In neonates with ALI/ARDS, HFOV combined with PS can improve pulmonary function more effectively and shorten the durations of mechanical ventilation and oxygen use compared with CMV+PS and CMV alone. It does not increase the incidence of complications.


Assuntos
Lesão Pulmonar Aguda/terapia , Ventilação de Alta Frequência , Surfactantes Pulmonares/uso terapêutico , Síndrome do Desconforto Respiratório do Recém-Nascido/terapia , Lesão Pulmonar Aguda/fisiopatologia , Terapia Combinada , Feminino , Humanos , Recém-Nascido , Masculino , Síndrome do Desconforto Respiratório do Recém-Nascido/fisiopatologia , Mecânica Respiratória
17.
Zhongguo Dang Dai Er Ke Za Zhi ; 18(9): 867-873, 2016 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-27655546

RESUMO

OBJECTIVE: To study the association between endoplasmic reticulum stress (ERS) pathway mediated by inositol-requiring kinase 1 (IRE1) and the apoptosis of type II alveolar epithelial cells (AECIIs) exposed to hyperoxia. METHODS: The primarily cultured AECIIs from preterm rats were devided into an air group and a hyperoxia group. The model of hyperoxia-induced cell injury was established. The cells were harvested at 24, 48, and 72 hours after hyperoxia exposure. An inverted phase-contrast microscope was used to observe morphological changes of the cells. Annexin V/PI double staining flow cytometry was performed to measure cell apoptosis. RT-PCR and Western blot were used to measure the mRNA and protein expression of glucose-regulated protein 78 (GRP78), IRE1, X-box binding protein-1 (XBP-1), and C/EBP homologous protein (CHOP). An immunofluorescence assay was performed to measure the expression of CHOP. RESULTS: Over the time of hyperoxia exposure, the hyperoxia group showed irregular spreading and vacuolization of AECIIs. Compared with the air group, the hyperoxia group showed a significantly increased apoptosis rate of AECIIs and significantly increased mRNA and protein expression of GRP78, IRE1, XBP1, and CHOP compared at all time points (P<0.05). The hyperoxia group had significantly greater fluorescence intensity of CHOP than the air group at all time points. In the hyperoxia group, the protein expression of CHOP was positively correlated with the apoptosis rate of AECIIs and the protein expression of IRE1 and XBP1 (r=0.97, 0.85, and 0.88 respectively; P<0.05). CONCLUSIONS: Hyperoxia induces apoptosis of AECIIs possibly through activating the IRE1-XBP1-CHOP pathway.


Assuntos
Apoptose , Estresse do Retículo Endoplasmático/fisiologia , Endorribonucleases/fisiologia , Hiperóxia/patologia , Complexos Multienzimáticos/fisiologia , Proteínas Serina-Treonina Quinases/fisiologia , Alvéolos Pulmonares/patologia , Animais , Células Cultivadas , Células Epiteliais/fisiologia , Feminino , Hiperóxia/metabolismo , Ratos , Ratos Sprague-Dawley , Fator de Transcrição CHOP/fisiologia , Proteína 1 de Ligação a X-Box/fisiologia
18.
J Int Med Res ; 44(5): 1131-1137, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27565746

RESUMO

Polyuria and polydipsia are the characteristics of congenital nephrogenic diabetes insipidus (CNDI). Approximately 90% of all patients with CNDI have X-linked hereditary disease, which is due to a mutation of the arginine vasopressin receptor 2 ( AVPR2) gene. This case report describes a 54-year-old male with polyuria and polydipsia and several male members of his pedigree who had the same symptoms. The proband was diagnosed with diabetes insipidus using a water-deprivation and arginine vasopressin stimulation test. Genomic DNA from the patient and his family members was extracted and the AVPR2 gene was sequenced. A novel missense mutation of a cytosine to guanine transition at position 972 (c.972C > G) was found, which resulted in the substitution of isoleucine for methionine at amino acid position 324 (p.I324M) in the seventh transmembrane domain of the protein. The proband's mother and daughter were heterozygous for this mutation. The novel mutation of the AVPR2 gene further broadens the phenotypic spectrum of the AVPR2 gene.


Assuntos
Substituição de Aminoácidos/genética , Diabetes Insípido Nefrogênico/genética , Receptores de Vasopressinas/genética , Grupo com Ancestrais do Continente Asiático , Sequência de Bases , Diabetes Insípido Nefrogênico/diagnóstico , Humanos , Masculino , Pessoa de Meia-Idade , Mutação de Sentido Incorreto/genética , Linhagem , Polidipsia/diagnóstico , Poliúria/diagnóstico
19.
ACS Appl Mater Interfaces ; 8(32): 20650-9, 2016 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-27454458

RESUMO

As a promising alternative for lithium ion batteries, room-temperature sodium ion batteries (SIBs) have become one significant research frontier of energy storage devices although there are still many difficulties to be overcome. For the moment, the studies still concentrate on the preparation of new electrode materials for SIBs to meet the applicability. Herein, one new P2-Na2/3Ni1/3Mn5/9Al1/9O2 (NMA) cathode material is successfully prepared via a simple and facile liquid-state method. The prepared NMA is layered transition metal oxide, which can keep stable crystal structure during sodiation/desodiation as demonstrated by the ex situ X-ray diffraction, and its electrochemical properties can be further enhanced by connecting the cake-like NMA microparticles with reduced graphene oxide (RGO) using a ball milling method. Electrochemical tests show that the formed RGO-connected NMA (NMA/RGO) can deliver a higher reversible capacity of up to 138 mAh g(-1) at 0.1 C and also exhibit a superior high-rate capabilities and cycling stability in comparison to pure NMA. The much improved properties should be attributed to the reduced particle size and improvement of electrical conductivity and apparent Na(+) diffusion due to RGO incorporation, which is comprehensively verified by the electrochemical technologies of galvanostatic intermittent titration technique, electrochemical impedance spectroscopy and cyclic voltammetry at various scan rate as well as ex-situ X-ray diffraction studies.

20.
Pediatr Neurol ; 61: 94-98.e1, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27353694

RESUMO

BACKGROUND: To determine the association of histologic chorioamnionitis (HCA) and fetal inflammatory response syndrome (FIRS) with brain injuries in infants born to mothers with preterm premature rupture of membranes. METHODS: A total of 103 singleton infants born to mothers with preterm premature rupture of membranes were enrolled. The placental inflammation was confirmed by HCA, and FIRS was defined in fetuses with preterm labor and an elevation of the fetal plasma interleukin-6 concentration. Examination of brain images was conducted to confirm the existence of brain injuries. Based on placental HCA and umbilical cord blood interleukin-6 level, all patients were divided into three groups: HCA(-)FIRS(+), HCA(+)FIRS(-), and HCA(+)FIRS(+). RESULTS: Among all infants with preterm premature rupture of membranes, 53.40% were exposed to HCA, 20.38% experienced FIRS, and the overall incidence of brain injuries was 38.83%. The incidence of brain injury in HCA(-)FIRS(+), HCA(+)FIRS(-), and HCA(+)FIRS(+) groups were 20.83%, 41.18%, and 76.19%, respectively. HCA at the advanced grades and stages was associated with increased risk of brain injury. Umbilical cord blood levels of interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor alpha (TNF-α), and granulocyte-colony stimulating factor (G-CSF) in premature infants with brain injuries were significantly higher than in those without brain injuries. Infants diagnosed with both HCA and FIRS showed significantly higher levels of IL-8, TNF-α, and G-CSF than those with HCA alone. CONCLUSIONS: Preterm infants exposed to severe chorioamnionitis had an increased risk of brain injury. IL-6, IL-8, TNF-α, and G-CSF in cord blood were associated with brain injuries in preterm infants and may be used as extradiagnostic criteria.


Assuntos
Lesões Encefálicas/sangue , Lesões Encefálicas/epidemiologia , Corioamnionite/sangue , Corioamnionite/epidemiologia , Recém-Nascido Prematuro/sangue , Adulto , Biomarcadores/sangue , Lesões Encefálicas/imunologia , Corioamnionite/imunologia , Corioamnionite/patologia , Feminino , Sangue Fetal/imunologia , Ruptura Prematura de Membranas Fetais , Humanos , Recém-Nascido de Baixo Peso/sangue , Recém-Nascido de Baixo Peso/imunologia , Recém-Nascido , Recém-Nascido Prematuro/imunologia , Masculino , Placenta/imunologia , Placenta/patologia , Gravidez , Estudos Prospectivos , Risco , Fatores de Risco
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