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1.
Biomed Pharmacother ; 125: 109845, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32058211

RESUMO

Osteoarthritis (OA), a progressive joint disorder, is principally characterized by the degeneration and destruction of the articular cartilage. Ellagic acid (EA), a natural polyphenol found in berries and nuts has shown potent anti-inflammatory effects, however, its effects and underlying mechanisms on OA have seldom been systematically illuminated. In this study, we reported the anti-inflammatory effects of Ellagic acid (EA) in the progression of OA in both in vitro and in vivo experiments. in vitro study, IL-1ß-induced expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), Nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2), and interleukin-6 (IL-6) were inhibited by Ellagic acid (EA). Moreover, Ellagic acid (EA) down-regulated the IL-1ß-stimulated matrix metalloproteinase-13 (MMP-13) and thrombospondin motifs 5 (ADAMTS-5) while up-regulated the collagen of type II and aggrecan. Mechanistically, we revealed that Ellagic acid (EA) suppressed nuclear factor kappa B (NF-κB) signaling in IL-1ß -induced chondrocytes. And Ellagic acid (EA)-induced protectiveness in OA development was also shown by the DMM model. Taken together, our data indicate that Ellagic acid (EA) may serve as a potential drug for OA treatment.

2.
Adv Mater ; : e1903759, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32078198

RESUMO

The efficacy of therapeutics for brain tumors is seriously hampered by multiple barriers to drug delivery, including severe destabilizing effects in the blood circulation, the blood-brain barrier/blood-brain tumor barrier (BBB/BBTB), and limited tumor uptake. Here, a sequential targeting in crosslinking (STICK) nanodelivery strategy is presented to circumvent these important physiological barriers to improve drug delivery to brain tumors. STICK nanoparticles (STICK-NPs) can sequentially target BBB/BBTB and brain tumor cells with surface maltobionic acid (MA) and 4-carboxyphenylboronic acid (CBA), respectively, and simultaneously enhance nanoparticle stability with pH-responsive crosslinkages formed by MA and CBA in situ. STICK-NPs exhibit prolonged circulation time (17-fold higher area under curve) than the free agent, allowing increased opportunities to transpass the BBB/BBTB via glucose-transporter-mediated transcytosis by MA. The tumor acidic environment then triggers the transformation of the STICK-NPs into smaller nanoparticles and reveals a secondary CBA targeting moiety for deep tumor penetration and enhanced uptake in tumor cells. STICK-NPs significantly inhibit tumor growth and prolong the survival time with limited toxicity in mice with aggressive and chemoresistant diffuse intrinsic pontine glioma. This formulation tackles multiple physiological barriers on-demand with a simple and smart STICK design. Therefore, these features allow STICK-NPs to unleash the potential of brain tumor therapeutics to improve their treatment efficacy.

3.
Nucleic Acids Res ; 48(D1): D1085-D1092, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31624841

RESUMO

To date, large amounts of genomic and phenotypic data have been accumulated in the fields of crop genetics and genomic research, and the data are increasing very quickly. However, the bottleneck to using big data in breeding is integrating the data and developing tools for revealing the relationship between genotypes and phenotypes. Here, we report a rice sub-database of an integrated omics knowledgebase (MBKbase-rice, www.mbkbase.org/rice), which integrates rice germplasm information, multiple reference genomes with a united set of gene loci, population sequencing data, phenotypic data, known alleles and gene expression data. In addition to basic data search functions, MBKbase provides advanced web tools for genotype searches at the population level and for visually displaying the relationship between genotypes and phenotypes. Furthermore, the database also provides online tools for comparing two samples by their genotypes and finding target germplasms by genotype or phenotype information, as well as for analyzing the user submitted SNP or sequence data to find important alleles in the germplasm. A soybean sub-database is planned for release in 3 months and wheat and maize will be added in 1-2 years. The data and tools integrated in MBKbase will facilitate research in crop functional genomics and molecular breeding.

4.
J Cell Mol Med ; 24(2): 1893-1905, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31837208

RESUMO

The balance between bone formation and bone resorption is closely related to bone homeostasis. Osteoclasts, originating from the monocyte/macrophage lineage, are the only cell type possessing bone resorption ability. Osteoclast overactivity is thought to be the major reason underlying osteoclast-related osteolytic problems, such as Paget's disease, aseptic loosening of prostheses and inflammatory osteolysis; therefore, disruption of osteoclastogenesis is considered a crucial treatment option for these issues. WKYMVm, a synthetic peptide, which is a potent FPR2 agonist, exerts an immunoregulatory effect. This peptide inhibits the production of inflammatory cytokines, such as (IL)-1ß and TNF-α, thus regulating inflammation. However, there are only few reports on the role of WKYMVm and FPR2 in osteoclast cytology. In the current study, we found that WKYMVm negatively regulates RANKL- and lipopolysaccharide (LPS)-induced osteoclast differentiation and maturation in vitro and alleviates LPS-induced osteolysis in animal models. WKYMVm down-regulated the expression of osteoclast marker genes and resorption activity. Furthermore, WKYMVm inhibited osteoclastogenesis directly through reducing the phosphorylation of STAT3 and NF-kB and indirectly through the CD9/gp130/STAT3 pathway. In conclusion, our findings demonstrated the potential medicinal value of WKYMVm for the treatment of inflammatory osteolysis.

5.
ACS Appl Mater Interfaces ; 12(3): 3474-3493, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31874023

RESUMO

Vasculogenesis (de novo formation of vessels) induced by endothelial progenitor cells (EPCs) is requisite for vascularized bone regeneration. However, there exist few available options for promoting vasculogenesis within artificial bone grafts except for exogenous EPC transplantation, which suffers from the source of EPC, safety, cost, and time concerns in clinical applications. This study aimed at endogenous EPC recruitment for vascularized bone regeneration by using a bioinspired EPC-induced graft. The EPC-induced graft was created by immobilizing two bioactive peptides, WKYMVm and YIGSR, on the surface of poly(ε-caprolactone) (PCL)/poliglecaprone (PGC) nanofibrous scaffolds via a polyglycolic acid (PGA)-binding peptide sequence. Remarkable immobilization efficacy of WKYMVm and YIGSR peptides and their sustained release (over 14 days) from scaffolds were observed. In vivo and in vitro studies showed robust recruitment of EPCs, which subsequently contributed to early vasculogenesis and ultimate bone regeneration. The dual-peptide-functionalized nanofibrous scaffolds proposed in this study provide a promising therapeutic strategy for vasculogenesis in bone defect repair.

6.
Neural Netw ; 123: 217-233, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31884182

RESUMO

Compressed sensing (CS) significantly accelerates magnetic resonance imaging (MRI) by allowing the exact reconstruction of image from highly undersampling k-space data. In this process, the high sparsity obtained by the learned dictionary and exploitation of correlation among patches are essential to the reconstructed image quality. In this paper, by a use of these two aspects, we propose a novel CS-MRI model based on analysis dictionary learning and manifold structure regularization (ADMS). Furthermore, a proper tight frame constraint is used to obtain an effective overcomplete analysis dictionary with a high sparsifying capacity. The constructed manifold structure regularization nonuniformly enforces the correlation of each group formed by similar patches, which is more consistent with the diverse nonlocal similarity in realistic images. The proposed model is efficiently solved by the alternating direction method of multipliers (ADMM), in which the fast algorithm for each sub-problem is separately developed. The experimental results demonstrate that main components in the proposed method contribute to the final reconstruction performance and the effectiveness of the proposed model.

7.
Sci Rep ; 9(1): 20181, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31882731

RESUMO

Pan evaporation (Epan) was regarded as a critical indicator of climate change, especially in the Qinghai-Tibet Plateau (QTP). By using the measured daily Epan data of 274 stations in the QTP from 1970 to 2017, the study detected abrupt changes in annual Epan series in different spatial scales, through integrating the Mann-Kendall abrupt change test, moving t-test and piecewise linear fitting model. Results showed that abrupt changes existed generally in the QTP where mean and trend abrupt changes were detected in 76.6% and 97.8% of 274 stations during the last half-century. Major abrupt change time of mean values and trends was respectively in around 1996, 1989 and 2007. In comparison, early abrupt changes were observed in the south (south of 30°N) and north (north of 35°N) but late ones in the midland (30-35°N). Corresponding to the low frequent behaviors, pan evaporation paradox only existed in the QTP as a whole in 1970-1990 and was not apparent at site scale, with less than 9.5% of 274 stations detected in different periods. The results confirmed prevailing abrupt change of pan evaporation and its distinct spatial pattern in the QTP.

8.
Environ Pollut ; 255(Pt 2): 113255, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31563784

RESUMO

As one of the most cost-effective and sustainable methods for contaminants' removal, sequestration and/or detoxification, phytoremediation has already captured comprehensive attention worldwide. Nevertheless, the accurate effects of various spatial pattern in enhancing phytoremediation efficiency is not yet clear, especially for the polluted mining areas. This study designed nine planting patterns (monocropping, double intercropping and triple intercropping) of three indigenous plant species (Setaria viridis (L.), Echinochloa crus-galli (L.) and Phragmites australis (Cav.) Trin. ex Steud.) to further explore the effects of plants spatial pattern on phytoremediation efficiency. Considering the uncertainties of the residual contaminants' concentration (RCC) caused by soil anisotropy, permeability and land types, the interval transformation was introduced into the plant uptake model to simulate the remediation efficiency. Then multi-criteria decision analysis (MCDA) were applied to optimal the planting patterns, with the help of criteria of (a) the amount of heavy metal absorption; (b) the concentration of residual contaminant in soil; (c) root tolerance of heavy metals; (d) the total investment cost. Results showed that (1) the highest concentrations of Zn, Cd, and Pb of the polluted area were 7320.02, 14.30, 1650.51 mg kg-1 (2) During the 180 days simulation, the highest RMSE of residue trace metals in soil are 3.02(Zn), 2.67(Pb), 2.89(Cd), respectively. (3) The result of IMCDA shows that the planting patterns of Setaria viridis, Echinochloa crus-galli and Phragmites australis in alternative a9 (269 mg kg-1 year-1) had the highest absorption rate of heavy metals compared with a7 (235 mg kg-1 year-1) and a2 (240 mg kg-1 year-1). After 20 years of remediation, the simulated RCC in a9 is far below the national standard, and the root toxicity is 0.12 (EC ≤ EC20). In general, the optimal alternative derived from interval residual contaminant concentration can effectively express the dynamic of contaminant distribution and then can be effectively employed to evaluate the sustainable remediation methods.


Assuntos
Biodegradação Ambiental , Metais Pesados/metabolismo , Plantas/metabolismo , Poluentes do Solo/metabolismo , Técnicas de Apoio para a Decisão , Metais Pesados/análise , Mineração , Plantas/efeitos dos fármacos , Poaceae , Solo/química , Poluentes do Solo/análise , Oligoelementos/análise
9.
Environ Sci Pollut Res Int ; 26(24): 24630-24644, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31240649

RESUMO

Trace metal element contamination in mining areas is always a huge environmental challenge for the global mining industry. In this study, an abandoned sphalerite mine near the Yanshan Mountains was selected as subject to evaluate the soil and water contamination caused by small-scale mining. The results show that (1) Pearson correlation matrix and principal component analysis (PCA) results reveal that Zn, Cu, Cd, and Pb were greatly affected by the operation of mines, especially mineral tailings. The contents of trace metal elements decrease with the increase of the distance from the mining area. Zinc, Pb, and Cd were discovered in almost all soil samples, and Zn accounted for about 80% of pollution of the topsoil. (2) The trace element pollution levels in the topsoil of the three villages were ranked as follows: Cd > Cu > Pb~Zn. The potential ecological risk of farmland around the mine ranges from lower to higher, with Cd being the most harmful. (3) Human health risk assessment results show that trace elements in the mining area pose obvious non-carcinogenic health risks to children while the risks to adults are not equally obvious. The carcinogenic risk of Cd and Cr is within a safe range and does not pose an obvious cancer risk to the population.


Assuntos
Poluentes do Solo/análise , Sulfetos/química , Oligoelementos/análise , Compostos de Zinco/química , Zinco/análise , Adulto , Criança , China , Ecologia , Poluição Ambiental/análise , Humanos , Mineração , Medição de Risco , Solo , Poluentes do Solo/química , Oligoelementos/química , Recursos Hídricos , Zinco/química
11.
BMC Nephrol ; 20(1): 135, 2019 04 18.
Artigo em Inglês | MEDLINE | ID: mdl-30999892

RESUMO

AIMS: Sphingosine-1-phosphate receptor 2 (S1PR2) is a G-protein-coupled receptor that regulates sphingosine-1-phosphate-triggered cellular response. However, the role of S1PR2 in diabetes-induced glomerular endothelial cell dysfunction remains unclear. This study aims to investigate the effect of S1PR2 blockade on the morphology and function of mitochondria in human renal glomerular endothelial cells (HRGECs). METHODS: HRGECs were pretreated with a S1PR2 antagonist (JTE-013) or a Rho-associated coiled coil-containing protein kinase 1 (ROCK1) inhibitor (Y27632) for 30 min and then cultured with normal glucose (5.5 mM) or high glucose (30 mM) for 72 h. The protein expression levels of RhoA, ROCK1, and Dynmin-related protein-1(Drp1) were evaluated by immunoblotting; mitochondrial morphology was observed by electron microscopy; intracellular levels of ATP, ROS, and Ca2+ were measured by ATPlite, DCF-DA, and Rhod-2 AM assays, respectively. Additionally, the permeability, apoptosis, and migration of cells were determined to evaluate the effects of S1PR2 and ROCK1 inhibition on high glucose-induced endothelial dysfunction. RESULTS: High glucose induced mitochondrial fission and dysfunction, indicated by increased mitochondrial fragmentation, ROS generation, and calcium overload but decreased ATP production. High glucose also induced endothelial cell dysfunction, indicated by increased permeability and apoptosis but decreased migration. However, inhibition of either S1PR2 or ROCK1 almost completely blocked these high glucose-mediated cellular responses. Furthermore, inhibiting S1PR2 resulted in the deceased expression of RhoA, ROCK1, and Drp1 while inhibiting ROCK1 led to the downregulated expression of Drp1. CONCLUSIONS: S1PR2 antagonist modulates the morphology and function of mitochondria in HRGECs via the positive regulation of the RhoA/ROCK1/Drp1 signaling pathway, suggesting that the S1PR2/ROCK1 pathway may play a crucial role in high glucose milieu.

12.
Chemosphere ; 225: 395-405, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30884301

RESUMO

The optimal treatment designs of the heavy metal pollution sites and the calculation of the recovery capacity are important in recent studies. In this paper, we aimed to model the accumulation of heavy metals under different artificially Cd added concentrations, and analyzed the various tobacco solute adsorption and fluid flow properties. The finite difference method was used to simulate the heavy metals flux and root absorption in the soil, and the model simulation was compared with the measured values to quantify the uncertainty of the metal transport and modeling parameters. Treatments with different Cd levels were compared, e.g., control tillage (CT), low Cd tillage (LT, 2.0 mg/kg), high Cd tillage (HT, 20.0 mg/kg), ultra-high Cd tillage (UHT, 80.0 mg/kg). The predicted soil water content (SWC) was consistent with observed data. Predicted cumulative root water uptake (mm) ranked as follows: CT (196)>LT (178)>HT (134)>UHT (117). Potential transpiration rates (T r p) under HT and UHT were lower than that of other treatment, because of their lower leaf Area Index (LAI). The predicted root Cd uptake showed a strong correlation within the actual Cd uptake. The predicted root absorption of Cdmax was UHT (180.17)> HT (106.52)> LT (53.20) >CT (0.610). However, deviation of models was added by the Cd effluent trend and the performance of root exudates. This finding would be useful for further investigation into bio-remediation in the agricultural area, not only for Cd ion but for a range of other heavy metal contaminants.


Assuntos
Transporte Biológico/fisiologia , Cádmio/metabolismo , Raízes de Plantas/metabolismo , Poluentes do Solo/análise , Tabaco/metabolismo , Adsorção/fisiologia , Agricultura , Atmosfera , Metais Pesados/análise , Folhas de Planta/química , Solo
13.
BMC Cancer ; 19(1): 181, 2019 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-30819119

RESUMO

BACKGROUND: Colorectal cancer (CRC) is one of the most common cancers worldwide, and genetic variations exert distinct roles in its pathogenesis. Single nucleotide polymorphisms (SNPs) in interleukin 1 alpha (IL1A) were reported to be correlated to the susceptibility of diverse cancers. The aim of this study was to assess the association of IL1A SNPs with the risk of colorectal cancer in a Chinese Han population. METHODS: To evaluate the correlation between IL1A polymorphisms and CRC risk, Agena MassARRAY platform was used for genotype determination among 248 CRC patients and 463 controls. The relationships between IL1A variants and CRC susceptibility were examined by logistic regression analysis. Stratified analysis was conducted for the association detection in males and females. Haplotype construction and analysis were applied to evaluate the potential relationship between the genetic block and the risk of CRC. SNP functional exploration was performed with available bioinformatics datasets. RESULTS: After adjusting for age and gender, the "AA" genotype of rs2856838 exhibited a risk association with colorectal cancer in the recessive model (adjusted OR = 1.98, 95% CI: 1.05-3.72, p = 0.036). With stratified analysis, the recessive models of rs3783550 (OR = 2.17, 95% CI: 1.03-4.60, p = 0.043), rs2856838 (OR = 2.58, 95% CI: 1.13-5.87, p = 0.024), rs1609682 (OR = 2.20, 95% CI: 1.04-4.65, p = 0.040), and rs3783521 (OR = 2.13, 95% CI: 1.01-4.49, p = 0.048) revealed significant relationships between these variants and an increased CRC risk only in females. Bioinformatics analysis also revealed the putative functions of the selected SNPs. CONCLUSIONS: This study demonstrated that rs2856838 could influence the susceptibility to CRC in Chinese Han population from northwest China. IL1A variants rs3783550, rs2856838, rs1609682, and rs3783521 were associated with CRC risk only in females.


Assuntos
Neoplasias Colorretais/epidemiologia , Neoplasias Colorretais/genética , Predisposição Genética para Doença , Interleucina-1alfa/genética , Polimorfismo de Nucleotídeo Único , Adulto , Idoso , Alelos , Estudos de Casos e Controles , China/epidemiologia , Mapeamento Cromossômico , Feminino , Frequência do Gene , Genótipo , Haplótipos , Humanos , Padrões de Herança , Desequilíbrio de Ligação , Masculino , Pessoa de Meia-Idade , Razão de Chances
14.
Mater Sci Eng C Mater Biol Appl ; 98: 1241-1251, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30813005

RESUMO

Studies have demonstrated that scaffolds, a component of bone tissue engineering, play an indispensable role in bone repair. However, these scaffolds involving ex-vivo cultivated cells seeded have disadvantages in clinical practice, such as limited autologous cells, time-consuming cell expansion procedures, low survival rate and immune-rejection issues. To overcome these disadvantages, recent focus has been placed on the design of functionalized cell-free scaffolds, instead of cell-seeded scaffolds, that can reduplicate the natural self-healing events of bone fractures, such as inflammation, cell recruitment, vascularization, and osteogenic differentiation. New approaches and applications in tissue engineering and regenerative medicine continue to drive the development of functionalized cell-free scaffolds for bone repair. In this review, the self-healing processes were highlighted, and approaches for the functionalization were summarized. Also, ongoing efforts and breakthroughs in the field of functionalization for bone defect repair were discussed. Finally, a brief summery and new perspectives for functionalization strategies were presented to provide guidelines for further efforts in the design of bioinspired cell-free scaffolds.


Assuntos
Regeneração Óssea/fisiologia , Osso e Ossos/citologia , Fraturas Ósseas/terapia , Osteogênese/fisiologia , Animais , Técnicas de Cultura de Células/métodos , Diferenciação Celular/fisiologia , Humanos , Medicina Regenerativa/métodos , Engenharia Tecidual/métodos , Tecidos Suporte
15.
Exp Ther Med ; 17(4): 2425-2432, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30906429

RESUMO

It is well known that the lipotoxic mechanism of palmitic acid (PA), a main constituent of triglyceride, is dependent on reactive oxygen species (ROS). Recently, it has also been reported that PA is an autophagy inducer. However, the causal association and underlying mechanism of induced autophagy and ROS in PA toxicity remain unclear. The present study demonstrates for the first time that PA-induced autophagy enhances ROS generation via activating the calcium ion/protein kinase Cα/nicotinamide adenine dinucleotide phosphate oxidase 4 (Ca2+/PKCα/NOX4) pathway in human umbilical vein endothelial cells (HUVECs). It was revealed that PA treatment resulted in a significant increase in ROS generation and autophagic activity, leading to endothelial dysfunction as indicated by downregulated nitric oxide synthesis, decreased capillary-like structure formation and damaged cell repair capability. Furthermore, PA effectively activated the Ca2+/PKCα/NOX4 pathway, which is indicative of upregulated cytosolic Ca2+ levels, activated PKCα and increased NOX4 protein expression. 3-Methyladenine was then used to inhibit autophagy, which significantly reduced PA-induced ROS generation and blocked the Ca2+/PKCα/NOX4 pathway. The endothelial dysfunction caused by PA was ameliorated by downregulating ROS generation using a NOX4 inhibitor. In conclusion, PA-induced autophagy contributes to endothelial dysfunction by increasing oxidative stress via the Ca2+/PKCα/NOX4 pathway in HUVECs.

16.
J Cell Mol Med ; 23(2): 798-810, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30444033

RESUMO

The dipeptidyl peptidase 4 inhibitor vildagliptin (VLD), a widely used anti-diabetic drug, exerts favourable effects on vascular endothelium in diabetes. We determined for the first time the improving effects of VLD on mitochondrial dysfunction in diabetic mice and human umbilical vein endothelial cells (HUVECs) cultured under hyperglycaemic conditions, and further explored the mechanism behind the anti-diabetic activity. Mitochondrial ROS (mtROS) production was detected by fluorescent microscope and flow cytometry. Mitochondrial DNA damage and ATP synthesis were analysed by real time PCR and ATPlite assay, respectively. Mitochondrial network stained with MitoTracker Red to identify mitochondrial fragmentation was visualized under confocal microscopy. The expression levels of dynamin-related proteins (Drp1 and Fis1) were determined by immunoblotting. We found that VLD significantly reduced mtROS production and mitochondrial DNA damage, but enhanced ATP synthesis in endothelium under diabetic conditions. Moreover, VLD reduced the expression of Drp1 and Fis1, blocked Drp1 translocation into mitochondria, and blunted mitochondrial fragmentation induced by hyperglycaemia. As a result, mitochondrial dysfunction was alleviated and mitochondrial morphology was restored by VLD. Additionally, VLD promoted the phosphorylation of AMPK and its target acetyl-CoA carboxylase in the setting of high glucose, and AMPK activation led to a decreased expression and activation of Drp1. In conclusion, VLD improves endothelial mitochondrial dysfunction in diabetes, possibly through inhibiting Drp1-mediated mitochondrial fission in an AMPK-dependent manner.

17.
Environ Sci Pollut Res Int ; 26(2): 1336-1354, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30426366

RESUMO

From the perspective of river basin refined management and pollution control of water bodies, a transboundary river basin and its regional pollutant sources are identified and the typical status of discharging processes of different pollutant sources are screened. Then organic connection which can comprehensively reflect and dynamically characterize the discharge of transboundary water pollutants is constructed. In addition, the integrated prediction (IP) model of the transboundary river basin and its regional water pollutants discharge is established. Finally, the dynamic simulation of typical status characteristics of the transboundary river basin and its regional pollutant sources discharge as well as the tempo-spatial changing pattern of pollutant discharge intensity is conducted in this paper. This paper selected the Songhua River basin as an example where planting, industry, household (urban living and rural living), and livestock and poultry are the main pollutant sources. The dynamic simulation of water pollution discharge in Songhua River basin during the 13th Five-year Plan and its tempo-spatial changing trend analysis are conducted by employing the established IP model of transboundary river basin water pollution discharge. The results show that during the 13th Five-year Plan, through comprehensive management and control of pollutant sources in Songhua River basin, the discharge amounts of different pollutant sources (planting, industry, household, livestock, and poultry) present an overall decreasing trend and the main pollutants discharge intensity decreases significantly year by year. It is demonstrated that pollution discharge in Songhua River basin is controlled effectively.


Assuntos
Monitoramento Ambiental , Poluentes Químicos da Água/análise , China , Rios
18.
World J Gastroenterol ; 24(39): 4499-4509, 2018 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-30356927

RESUMO

AIM: To analyse the postoperative survival of patients with portal hypertension and determine the factors that influence survival and construct nomograms. METHODS: We retrospectively followed 1045 patients who underwent splenectomy plus pericardial devascularisation (SPD) between January 2002 and December 2017. Two SPD types are used in our department: splenectomy plus simplified pericardial devascularisation (SSPD) and splenectomy plus traditional pericardial devascularisation (STPD). The Kaplan-Meier method and Cox regression analysis were used to evaluate the prognostic effects of multiple parameters on overall survival (OS), disease-specific survival (DSS) and bleeding-free survival (BFS). Significant prognostic factors were combined to build nomograms to predict the survival rate of individual patients. RESULTS: Five hundred and fifty-seven (53.30%) patients were successfully followed with 192 in the SSPD group and 365 in the STPD group; 93 (16.70%) patients died, of whom 42 (7.54%) died due to bleeding. Postoperative bleeding was observed in 84 (15.10%) patients. The 5- and 10-year OS, DSS and BFS rates in the group of patients who underwent SSPD were not significantly different from those in patients who underwent STPD. Independent prognostic factors for OS were age, operative time, alanine transaminase level and albumin-bilirubin score. Independent prognostic factors for BFS were male sex, age, intraoperative blood loss and time to first flatus. Independent prognostic factors for DSS were the Comprehensive Complication Index and age. These characteristics were used to establish nomograms, which showed good accuracy in predicting 1-, 3- and 5-year OS and BFS. CONCLUSION: SSPD achieves or surpasses the long-term survival effect of traditional pericardial devascularisation and is worthy of clinical promotion and application. Nomograms are effective at predicting prognosis.


Assuntos
Hipertensão Portal/mortalidade , Hipertensão Portal/cirurgia , Nomogramas , Hemorragia Pós-Operatória/mortalidade , Esplenectomia/efeitos adversos , Adulto , Fatores Etários , Perda Sanguínea Cirúrgica/estatística & dados numéricos , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Pericárdio/cirurgia , Hemorragia Pós-Operatória/etiologia , Período Pós-Operatório , Estudos Retrospectivos , Fatores Sexuais , Esplenectomia/métodos , Análise de Sobrevida , Taxa de Sobrevida , Resultado do Tratamento
19.
Onco Targets Ther ; 11: 5407-5417, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30214246

RESUMO

Background: The present meta-analysis was aimed to evaluate the effects of postoperative adjuvant chemotherapy/transarterial chemoembolization (TACE) on the survival/disease-free survival (DFS) rate in hepatocellular carcinoma (HCC) patients with portal vein tumor thrombosis (PVTT). Methods: The relevant trials were collected using a database search of MEDLINE, Embase, Cochrane Library, Web of Science, ScienceDirect, the China Journal Full-text Database, and the National Institute of Health Clinical Trials Database. The 1-, 3-, and 5-year survival/DFS rates were considered to be the primary end points. A sensitivity analysis was conducted by reanalyzing the data using different statistical approaches. Results: Eight studies met the inclusion criteria. When compared with surgery alone, the pooled OR showed that the postoperative adjuvant therapy significantly increased the 1-, 3-, and 5-year survival rates for HCC patients with PVTT (the pooled OR and 95% CI of the 1-, 3-, and 5-year survival rates, respectively, were as follows: 2.72, 1.98-3.74; 1.62, 1.13-2.33; 1.99, 1.20-3.29). In addition, when compared with surgery alone, subgroup analysis showed that the postoperative chemotherapy improved the 1-, 3-, and 5-year survival rates of HCC patients with PVTT. Conclusion: Compared with surgery alone, postoperative adjuvant chemotherapy can improve the 1-, 3- and 5-year survival rates of HCC patients with PVTT. However, postoperative TACE can only increase the 1-year survival rate. However, due to the limitations of this meta-analysis, additional relevant trials are required to confirm these findings.

20.
World J Clin Cases ; 6(6): 99-109, 2018 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-29988874

RESUMO

AIM: To compare the clinical outcomes of patients with portal hypertension (PH) who underwent treatment with splenectomy plus simplified pericardial devascularisation (SSPD) or splenectomy plus traditional pericardial devascularisation (STPD). METHODS: We conducted a single-centre retrospective study of 1045 PH patients treated with either SSPD (S Group, 357 patients) or STPD (T Group, 688 patients) between January 2002 and February 2017. In all, 37 clinical indicators were compared to evaluate the efficacy of SSPD. RESULTS: Perioperative indicators in the S Group were significantly better than those in the T Group (P < 0.05). In both groups, the postoperative long-term portal vein diameter and Model for End-Stage Liver Disease score were significantly lower than those in the preoperative and postoperative short-term groups (P < 0.05). The incidence of complications in the S Group was significantly lower than that in the T Group (P < 0.05). Compared to the T Group, postoperative short-term WBC (white blood cell) and platelet counts were significantly lower and the short-term Hb (haemoglobin) level was significantly higher in the S Group (P < 0.05). In the S Group, postoperative long-term total bilirubin, direct bilirubin, alanine transaminase, and aspartate transaminase and postoperative serum creatinine and cystatin C levels were significantly lower than those in the T Group (P < 0.05), and postoperative albumin was significantly higher than that in the T Group (P < 0.05). CONCLUSION: Compared to STPD, SSPD is a simple and easy procedure resulting in less tissue damage. Patients recovered smoothly and steadily with fewer complications. Short-term liver and kidney function damage was less severe, and long-term liver function recovery was better. Therefore, SSPD is worthy of clinical promotion and application for the treatment of PH.

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