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1.
Artigo em Inglês | MEDLINE | ID: mdl-36640438

RESUMO

Electrolyte engineering is crucial for the commercialization of lithium metal batteries. Here, lithium metal is stabilized in highly reactive sulfolane-based electrolyte under low concentration (0.25 M) for the first time. Inorganic-polymer hybrid SEI with high ionic conductivity, low bonding with lithium and high flexibility enables dense chunky lithium deposition and high plating/stripping efficiency. LCE also enables excellent cycling stability of NCM523/Li cells at 1 C (90.7% retention after 500 cycles) and 0.3 C (83.3% retention after 1000 cycles). With a low N/P ratio (~2), the capacity retention for NCM523/Li cells can achieve 94.3% after 100 cycles at 0.3 C. The exploring of the LCE is of paramount significance because it provides more possibilities of the lithium salts selection, especially reviving some lithium salts that are excluded before due to their low solubility. More importantly, LCE has the significant advantage of commercialization due to the cost effectiveness.

2.
Adv Mater ; : e2210658, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36641734

RESUMO

The CO2 utilization and conversion are of great importance in alleviating the rising CO2 concentration in the atmosphere. Here, we report a single-atom catalyst (SAC) for electrochemical CO2 utilization in both the aqueous and the aprotic electrolyte. Specifically, the atomically dispersed Mn-N4 sites are embedded in bowl-like mesoporous carbon particles with the functionalization of epoxy groups in the second coordination spheres. Theoretical calculations suggest that the epoxy groups near the Mn-N4 site adjust the electronic structure of the catalyst with reduced reaction energy barriers for the electrocatalytic reduction of CO2 to CO. The resultant MCs-(N,O) catalyst exhibits extraordinary electrocatalytic performance with a high CO faradaic efficiency of 94.5%, a high CO current density of 13.7 mA cm-2 , and a low overpotential of 0.44 V in the aqueous environment. Meanwhile, as a cathode catalyst for aprotic Li-CO2 batteries, the MCs-(N,O) with well-regulated active sites and unique mesoporous bowl-like morphology optimizes the nucleation behavior of discharge products. MCs-(N,O)-based batteries deliver a low overpotential and excellent cyclic stability of 1000 h. Findings in this work provide a new avenue to design and fabricate SACs for various electrochemical CO2 utilization systems. This article is protected by copyright. All rights reserved.

3.
Trials ; 24(1): 13, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36609307

RESUMO

BACKGROUND: Although opioids are commonly prescribed in clinical anaesthesia, the significant side effects attributed to their overuse are raising increasing concerns. One way to reduce perioperative opioid consumption is to apply opioid-reduced anaesthesia (ORA) and even opioid-free anaesthesia (OFA), which involves regional techniques, neuraxial anaesthesia, nonopioid analgesics or combined use. The aim of this study was to investigate whether the application of OFA by using esketamine in intraoperative analgesia could minimize the side effects of postoperative nausea and vomiting (PONV), as well as other short-term side effects related to anaesthesia. METHODS/DESIGN: The study was designed as a prospective, randomized, controlled, multicentre trial. A total of 278 patients were enrolled; participants were nonsmoking female patients aged 18-50 years and scheduled for laparoscopic appendectomy or cholecystectomy, ASA at I-III, with no serious physical or mental diseases. Both groups received usual perioperative care except for the analgesic medication of either esketamine or sufentanil. The primary outcome was the incidence of PONV 3 days after surgery. Secondary outcomes included recovery status, pain, sedation level and overall recovery, delirium and cognition, anxiety and depression and total consumption of analgesic agents. DISCUSSION: This trial may show that the synergy of esketamine and propofol anaesthesia reduces PONV as well as other short-term adverse events, thereby providing a better safety and satisfaction profile of ERAS for laparoscopic appendectomy and cholecystectomy. TRIAL REGISTRATION: Chinese Clinical Trial Registry ChiCTR2100047169. Registered on June 9, 2021.


Assuntos
Anestesia , Laparoscopia , Humanos , Feminino , Náusea e Vômito Pós-Operatórios/prevenção & controle , Analgésicos Opioides/efeitos adversos , Estudos Prospectivos , Analgésicos/uso terapêutico , Laparoscopia/efeitos adversos , Dor Pós-Operatória/diagnóstico , Dor Pós-Operatória/tratamento farmacológico , Dor Pós-Operatória/etiologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Estudos Multicêntricos como Assunto
4.
Cell Discov ; 9(1): 3, 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36609558

RESUMO

SARS-CoV-2 Omicron subvariants have demonstrated extensive evasion from monoclonal antibodies (mAbs) developed for clinical use, which raises an urgent need to develop new broad-spectrum mAbs. Here, we report the isolation and analysis of two anti-RBD neutralizing antibodies BA7208 and BA7125 from mice engineered to produce human antibodies. While BA7125 showed broadly neutralizing activity against all variants except the Omicron sublineages, BA7208 was potently neutralizing against all tested SARS-CoV-2 variants (including Omicron BA.1-BA.5) except Mu. By combining BA7208 and BA7125 through the knobs-into-holes technology, we generated a biparatopic antibody BA7208/7125 that was able to neutralize all tested circulating SARS-CoV-2 variants. Cryo-electron microscopy structure of these broad-spectrum antibodies in complex with trimeric Delta and Omicron spike indicated that the contact residues are highly conserved and had minimal interactions with mutational residues in RBD of current variants. In addition, we showed that administration of BA7208/7125 via the intraperitoneal, intranasal, or aerosol inhalation route showed potent therapeutic efficacy against Omicron BA.1 and BA.2 in hACE2-transgenic and wild-type mice and, separately, effective prophylaxis. BA7208/7125 thus has the potential to be an effective candidate as an intervention against COVID-19.

5.
Int J Nanomedicine ; 18: 263-276, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36660338

RESUMO

Background: Nanocarrier platforms have been indicated to have great potential in clinical practice to treat non-small cell lung cancer (NSCLC). Our previous Phase III clinical study revealed that polymeric micellar paclitaxel (Pm-Pac) is safe and efficacious in advanced NSCLC patients. However, the histopathological-toxicological profile of Pm-Pac in mammals remains unclear. Methods: We examined the Pm-Pac-induced antitumour effect in both A549/H226 cells and A549/H226-derived xenograft tumour models.. And then, we evaluated the short-term and long-term toxicity induced by Pm-Pac in healthy Sprague‒Dawley (SD) rats. The changes in body weight, survival, peripheral neuropathy, haematology, and histopathology were studied in SD rats administered Pm-Pac at different dosages. Results: In the A549-derived xenograft tumour model, better therapeutic efficacy was observed in the Pm-Pac group than in the solvent-based paclitaxel (Sb-Pac) group when an equal dosage of paclitaxel was administered. Toxicity assessments in healthy SD rats indicated that Pm-Pac caused toxicity at an approximately 2- to 3-fold greater dose than Sb-Pac when examining animal body weight, survival, peripheral neuropathy, haematology, and histopathology. Interestingly, based on histopathological examinations, we found that Pm-Pac could significantly decrease the incidences of paclitaxel-induced brain and liver injury but could potentially increase the prevalence of paclitaxel-induced male genital system toxicity. Conclusion: This study introduces the toxicological profile of the engineered nanoparticle Pm-Pac and provides a novel perspective on the Pm-Pac-induced histopathological-toxicological profile in a rat model.


Assuntos
Antineoplásicos Fitogênicos , Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Nanopartículas , Doenças do Sistema Nervoso Periférico , Ratos , Humanos , Masculino , Animais , Paclitaxel/farmacologia , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Micelas , Neoplasias Pulmonares/tratamento farmacológico , Ratos Sprague-Dawley , Polímeros , Modelos Animais de Doenças , Nanopartículas/toxicidade , Doenças do Sistema Nervoso Periférico/tratamento farmacológico , Peso Corporal , Mamíferos
6.
Biotechnol J ; : e2200470, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36683552

RESUMO

Bone morphogenetic protein-2 (BMP-2) and transforming growth factor-ß1 (TGF-ß1) reportedly induce the osteogenic and tenogenic differentiation of anterior cruciate ligament (ACL)-derived stem cells (LDSCs), respectively. However, few studies have investigated the effect of BMP-2/TGF-ß1 on the differentiation of LDSC. We developed a BMP-2/TGF-ß1 gene insertion into an LDSC cell sheet that promotes tendon-bone healing in a mouse ACL reconstruction (ACLR) model.. CD34+ LDSCs were isolated from human ACL stump tissues, virally transduced to express BMP-2 or TGF-ß1, and then embedded within cell sheets. All mice underwent ACLR using an autograft wrapped with a cell sheet and were randomly divided into 3 groups: BMP-2-, TGF-ß1-, and BMP-2/TGF-ß1-transduced. At 4 and 8 weeks, tendon-bone healing was evaluated by micro-CT, biomechanical test, and histological analysis. BMP-2 and TGF-ß1 promoted the osteogenic and tenogenic differentiation of LDSC in vitro. BMP-2/TGF-ß1-transduced LDSC sheet application contributed to early improvement in mean failure load and graft stiffness, accelerated maturation of the tendon-bone junction, and inhibited bone tunnel widening. Furthermore, reduced M1 macrophage infiltration and a higher M2 macrophage percentage were observed in the BMP-2/TGF-ß1-transduced LDSC group. This work demonstrated that BMP-2 and TGF-ß1 promoted CD34+ LDSCs osteogenic and tenogenic differentiation in vitro and in vivo, which accelerated the tendon-bone healing after ACLR using autografts wrapped with cell sheets in a mouse model. This article is protected by copyright. All rights reserved.

7.
Sensors (Basel) ; 23(2)2023 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-36679777

RESUMO

A fully transportable laser heterodyne radiometer (LHR), involving a flexible polycrystalline mid-infrared (PIR) fiber-coupling system and operating around 8 µm, was characterized and optimized with the help of a calibrated high temperature blackbody source to simulate solar radiation. Compared to a mid-IR free-space sunlight coupling system, usually used in a current LHR, such a fiber-coupling system configuration makes the mid-infrared (MIR) LHR fully transportable. The noise sources, heterodyne signal, and SNR of the MIR LHR were analyzed, and the optimum operating local oscillator (LO) photocurrent was experimentally obtained. The spectroscopic performance of the MIR LHR was finally evaluated. This work demonstrated that the developed fully transportable MIR LHR could be used for ground-based atmospheric sounding measurements of multiple trace gases in the atmospheric column. In addition, it also has high potential for applications on spacecraft or on an airborne platform.


Assuntos
Lasers , Radiometria , Análise Espectral/métodos , Gases , Luz
8.
BMC Ophthalmol ; 23(1): 20, 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36635644

RESUMO

PURPOSE: To report a novel surgical approach for the scleral fixation of the Rayner 620 H intraocular lens (IOL) with Gore-Tex suture and its outcomes at 6 months postoperatively. METHODS: 19 consecutive patients who underwent novel surgical approach for the scleral fixation of Rayner 620 H IOL with Gore-Tex suture at Peking Union Medical College Hospital between June 2020 and June 2021 were included. Data on best-corrected visual acuity (BCVA), spherical equivalent, total astigmatism/axis, short-term and long-term complications, and corresponding management with a follow-up of 6 months were collected. RESULTS: Nineteen patients (11 men and 8 women) with a mean age of 62.7 ± 10.6 years were included. The median BCVA improved significantly from 0.90 ± 0.90 (Snellen 20/160) preoperatively to 0.20 ± 0.30 (Snellen 20/32) at postoperative 6 months follow-up (P < 0.001). The stratification of the accuracy of refractive outcomes was 53% of patients within ± 0.5 D and 84% of patients within ± 1.0 D of the refractive target. Corneal edema (n = 3, 16%) and increased intraocular pressure (IOP) (n = 4, 11%) were short-term complications. Long-term complications included increased IOP (n = 1, 5%), and macular edema (n = 1, 5%). CONCLUSION: The novel surgical approach for scleral fixation of the Rayner 620 H IOL with Gore-Tex suture is a reasonable option for patients who need secondary IOL placement without adequate capsular support.


Assuntos
Lentes Intraoculares , Politetrafluoretileno , Masculino , Humanos , Feminino , Pessoa de Meia-Idade , Idoso , Estudos Retrospectivos , Implante de Lente Intraocular , Suturas , Esclera/cirurgia , Técnicas de Sutura , Complicações Pós-Operatórias/cirurgia
9.
J Phys Chem Lett ; 14(2): 481-488, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36625782

RESUMO

The development of abundant, cheap, and highly active catalysts for the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is important for hydrogen production. Nanolaminate ternary transition metal carbides (MAX phases) and their derived two-dimensional transition metal carbides (MXenes) have attracted considerable interest for electrocatalyst applications. Herein, four new MAX@MXene core-shell structures (Ta2CoC@Ta2CTx, Ta2NiC@Ta2CTx, Nb2CoC@Nb2CTx, and Nb2NiC@Nb2CTx), in which the core region is Co/Ni-MAX phases while the edge region is MXenes, have been prepared. Under alkaline electrolyte conditions, the Ta2CoC@Ta2CTx core-shell structure showed an overpotential of 239 mV and excellent stability during the HER with MXenes as the active sites. For the OER, the Ta2CoC@Ta2CTx core-shell structure showed an overpotential of 373 mV and a small Tafel plot (56 mV dec-1), which maintained a bulk crystalline structure and generated Co-based oxyhydroxides that formed by surface reconstruction as active sites. Considering rich chemical compositions and structures of MAX phases, this work provides a new strategy for designing multifunctional electrocatalysts and also paves the way for further development of MAX phase-based materials for clean energy applications.

10.
Adv Mater ; : e2209948, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36652951

RESUMO

Single-atom catalysts (SACs) show great potential for rechargeable Zn-air batteries (ZABs); however, scalable production of SACs from sustainable resources is difficult owing to poor control of the local coordination environment. Herein, lignosulfonate, a by-product of the papermaking industry, is utilized as a multifunctional bioligand for the mass production of SACs with highly active M-N4 -S sites (M represents Fe, Cu, and Co) via strong metal-nitrogen/sulfur coordination. This effectively adjusts the charge distribution and promotes the catalytic performance, leading to highly durable and excellent performance in oxygen reduction and evolution reactions for ZABs. This study paves the way for the industrial production of cost-effective SACs in a sustainable manner. This article is protected by copyright. All rights reserved.

11.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 54(1): 1-6, 2023 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-36647635

RESUMO

As one of the most diverse microbial communities within the human body, the oral microbiome is an important component that contributes to the maintenance of human health. The microbial composition of different sites in the oral cavity varies significantly and a dynamic equilibrium is maintained through communications with the environment and oral and distal organs of the host. It has been reported that there is significant correlation between dysbiotic oral microbiome and the occurrence or progression of a variety of systemic diseases. In this review, we summarized recent advances in research on the relationship between oral microbiome and systemic health, focusing on the interaction and pathological mechanisms between oral microbiome and systemic health and hoping to provide new avenues for the early prevention and clinical diagnosis and treatment of systemic diseases.


Assuntos
Microbiota , Humanos , Boca , Disbiose
12.
Adv Mater ; : e2210966, 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36649735

RESUMO

Increasing the upper cut-off voltage of LiCoO2 (LCO) is one of the most efficient strategies to gain high-energy density for current lithium-ion batteries. However, surface instability is expected to be exaggerated with increasing voltage arising from the high reactivity between the delithiated LCO and electrolytes, leading to serious safety concerns. Herein, we aim to construct a physical and chemical stable phosphate-rich cathode-electrolyte interface (CEI) on the LCO particles to mitigate this issue. This phosphate-rich CEI is generated during the electrochemical activation by using fluoroethylene carbonate and 1,1,2,2-tetrafluoroethyl-2,2,3,3-tetrafluoropropyletherare as the solvents. Both solvents also demonstrate high thermal stability, reducing the intrinsic flammability of the commercial organic electrolyte, thereby eliminating the safety concern in the LCO-based systems upon high-voltage operation. This stable CEI layer on the particle surface can also enhance the surface structure by blocking direct contact between LCO and electrolyte, improving the cycling stability. Therefore, by using the proposed electrolyte, the LCO cathode exhibits a high-capacity retention of 76.1% after 200 cycles at a high cut-off voltage of 4.6 V. This work provides a novel insight into the rational design of high-voltage and safe battery systems by adopting the flame-retardant electrolyte. This article is protected by copyright. All rights reserved.

13.
J Clin Pathol ; 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36650045

RESUMO

AIMS: In recent years, patients with programmed cell death-Ligand 1 (PD-L1)-positive oesophageal squamous cell carcinoma (OSCC) have been able to benefit from immunotherapy. However, method for improving the treatment efficacy of PD-L1-positive patients is a problem that needs further consideration. Studies on the relationship between human epidermal growth factor receptor 2 (HER2) and PD-L1 expression have recently been reported in certain cancers, but the relationship between PD-L1 and HER2 expression in OSCC is still unclear. METHODS: A total of 263 patients with OSCC were included in the study. PD-L1 protein expression and HER2 protein expression were analysed by immunohistochemistry (IHC), and fluorescence in situ hybridisation (FISH) was performed to assess HER2 gene amplification. The significance of differences between HER2 status, PD-L1 status and clinicopathological parameters was assessed. The relationship between PD-L1 status and HER2 status was examined. RESULTS: Of the 263 OSCC cases, the PD-L1-positive expression rates were 39.2% and 77.2% in OSCC for Tumour Proportion Score (TPS) and Combined Positive Score (CPS), respectively, and PD-L1 expression was associated with the degree of tumour differentiation. The HER2 expression was positive in 24% (63/263) of cases based on IHC and FISH. HER2 expression was not significantly associated with clinicopathological characteristics. PD-L1 TPS expression and CPS expression were significantly positively correlated with HER2 expression in OSCC. CONCLUSIONS: PD-L1 expression was significantly positively correlated with HER2 expression in OSCC. The results provide valuable insight for the future application of HER2-targeted therapy combined with immunotherapy in OSCC.

14.
PLoS One ; 18(1): e0280067, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36630442

RESUMO

COVID-19 has brought a great challenge to the medical system. A key scientific question is how to make a balance between home quarantine and staying in the hospital. To this end, we propose a game-based susceptible-exposed-asymptomatic -symptomatic- hospitalized-recovery-dead model to reveal such a situation. In this new framework, time-varying cure rate and mortality are employed and a parameter m is introduced to regulate the probability that individuals are willing to go to the hospital. Through extensive simulations, we find that (1) for low transmission rates (ß < 0.2), the high value of m (the willingness to stay in the hospital) indicates the full use of medical resources, and thus the pandemic can be easily contained; (2) for high transmission rates (ß > 0.2), large values of m lead to breakdown of the healthcare system, which will further increase the cumulative number of confirmed cases and death cases. Finally, we conduct the empirical analysis using the data from Japan and other typical countries to illustrate the proposed model and to test how our model explains reality.


Assuntos
COVID-19 , Humanos , COVID-19/epidemiologia , SARS-CoV-2 , Pandemias , Atenção à Saúde , Hospitais
15.
Natl Sci Rev ; 10(1): nwac171, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36684521

RESUMO

Two-dimensional covalent organic frameworks are promising for photocatalysis by virtue of their structural and functional diversity, but generally suffer from low activities relative to their inorganic competitors. To fulfill their full potential requires a rational tailoring of their structures at different scales as well as their surface properties. Herein, we demonstrate benzobisthiazole-based covalent organic frameworks as a superior photocatalyst for hydrogen production. The product features high crystallinity with ordered 2.5-nm-wide cylindrical mesopores and great water wettability. These structural advantages afford our polymeric photocatalyst with fast charge carrier dynamics as evidenced by a range of spectroscopic characterizations and excellent catalytic performances when suspended in solution or supported on melamine foams. Under visible-light irradiation, it enables efficient and stable hydrogen evolution with a production rate of 487 µmol h-1 (or a mass-specific rate of 48.7 mmol g-1 h-1)-far superior to the previous state of the art. We also demonstrate that hydrogen production can be stoichiometrically coupled with the oxidation conversion of biomass as exemplified by the conversion of furfuryl alcohol to 2-furaldehyde.

16.
Food Sci Nutr ; 11(1): 334-343, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36655102

RESUMO

Over the past few decades, more alcohol-problem concerns focused on reducing the risk of hangover caused by the alcoholic beverages over-consumption. Chinese distilled spirits (Baijiu) is one of the most favorite alcoholic beverages. The intention of this study is to explore the associations of main flavor components in Baijiu and hangover symptoms using mice acute alcohol withdrawal model. The behaviors of each mouse were assessed by open-field tests using separate groups of mice with the treatment of sauce-aroma Baijiu, light-aroma Baijiu, strong-aroma Baijiu, pure alcohol, and distilled water, respectively. The behavioral data including total move distance and immobile time were used as indicators for the evaluation of the liquor intoxicating effects. Alcohol and acetaldehyde concentrations in mice plasma and the neurotransmitter contents of GABA and Glu in mice cerebellum were detected afterward. The results showed that the mice with the treatment of Baijiu samples displayed unusual exciting behaviors including increased alcohol metabolization with alleviating drunken and hangover symptoms, compared with that of pure alcohol control groups after 2-4 h. Moreover, the sauce-aroma Baijiu treatment group showed lessening intoxicated symptoms than those of light-aroma Baijiu and strong-aroma Baijiu. In addition, there were significant differences between Baijiu and pure alcohol treatment groups at the inhibitory neurotransmitter GABAergic levels and its receptor GABA-AR1 activating levels in the mice neuron cells. Furthermore, the Principal Component Analysis (PCA) analysis inferred that the flavor compounds acetic acid, ethyl acetate, ethyl lactate, and 1-propanol in the sauce-aroma Baijiu were played the major roles in the drunk behaviors that caused by the hangover. While, the acetic acid in the sauce-aroma Baijiu was speculated as a major flavor component to accelerate the alcohol metabolism and retard hangover symptoms.

17.
Biomed Pharmacother ; 159: 114267, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36669363

RESUMO

BACKGROUND: Maternal diabetes compromises the quality and developmental potential of oocytes. Therefore, it is important to study how to ameliorate the adverse effects of diabetes on oocyte quality. Epigallocatechin gallate (EGCG) has a variety of physiological activities, including anti-inflammatory, antioxidant, and anti-diabetes. In the present study, we evaluated the effect of EGCG on the maturation of diabetic oocytes in vitro. OBJECTIVE: Investigating the role of EGCG in restoring the adverse effects of diabetes on oocyte quality. METHODS: Diabetes mouse model was established by a single injection of streptozotocin (STZ). Oocytes were collected and matured in vitro with/without EGCG in M16 medium. RESULTS: Compared with control, diabetic oocytes have a higher frequency of spindle defects and chromosome misalignment, but EGCG effectively reduces the incidence of oocytes with abnormal spindle assembly and chromosome mismatches. Moreover, the abnormal mitochondrial membrane potential (MMP) of diabetic oocytes is significantly alleviated by EGCG, and the reduced expression of genes regulating mitochondrial fusion (Mfn1 and Mfn2) and fission (Drp1) in diabetic oocytes is significantly increased while EGCG is added. EGCG also decreases the higher level of reactive oxygen species (ROS) in diabetic oocytes that may be regulated by the increased expression of superoxide dismutase 1 (Sod1) and superoxide dismutase 2 (Sod2). EGCG can also reduce the DNA damage of diabetic oocytes. CONCLUSIONS: Our results suggest that EGCG, at least partially, improve the quality of diabetic oocytes.

18.
Biol Psychiatry ; 93(3): 260-267, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36567086

RESUMO

BACKGROUND: Emotion regulation (ER) processes help support well-being, but ineffective ER is implicated in several psychiatric disorders. Engaging ER flexibly by going online and offline as needs and capacities shift may be more effective than engaging ER rigidly across time. Here, we sought to observe the neural temporal dynamics of an ER process, reappraisal, during regulation of responses to negative memories in healthy control subjects (n = 33) and subjects with major depressive disorder (n = 36). METHODS: To track the temporal dynamics of reappraisal neural systems, we used a functional magnetic resonance imaging neural decoding approach. In task 1, subjects explicitly engaged reappraisal on instruction in response to aversive images, and we used this task to develop the decoder for detecting reappraisal. In task 2, subjects experienced negative autobiographical memories from a distant (third person, ER condition) or immersed (first person, control condition) perspective. RESULTS: The neural decoder, trained to detect reappraisal in task 1, predicted greater reappraisal occurring during the task 2 distance versus immerse trials and was engaged more intensely during memories that were rated as being more negative. Across time, decoder output manifested a temporal dynamic of early engagement followed by disengagement. These results were replicated in an independent subject dataset (n = 59). Relative to healthy control subjects, subjects with major depressive disorder had a comparable initial increase in decoder engagement at the beginning of the trial but an attenuated decrease at the end. CONCLUSIONS: Subjects with major depressive disorder evidenced a more rigid neural dynamic of reappraisal compared with healthy control subjects. Rigid ER may indicate diminished ability to flexibly and effectively regulate emotion.


Assuntos
Transtorno Depressivo Maior , Regulação Emocional , Humanos , Depressão , Emoções/fisiologia , Afeto/fisiologia , Imageamento por Ressonância Magnética/métodos
19.
Lung Cancer ; 175: 79-87, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36481678

RESUMO

INTRODUCTION: The Pathology Committee of the International Association for the Study of Lung Cancer (IASLC) proposed a new histological grading system based on the combination of predominant and high-grade patterns in 2020. MATERIALS AND METHODS: Pathological sections from 631 patients with stage I-III invasive lung adenocarcinoma were reviewed. We then determined the histological grade according to the new grading system and confirmed the pathological features that included the filigree micropapillary and discohesive growth pattern. Applying of the novel IASLC grading system in prognosis stratification was verified and the clinical significance of the pathological characteristics was explored. RESULTS: Cox multivariable analysis revealed that in the stage I-III invasive lung adenocarcinoma, the IASLC grading system was significantly associated with disease-free survival (DFS) [hazard ratio (HR) = 1.419; 95 % confidence interval (CI): 1.040-1.937; P = 0.027] and overall survival (OS) (HR = 1.899; 95 % CI: 1.168-3.087; P = 0.010). In patients with IASLC Grades 1 and 2, the simultaneous presence of filigree micropapillary and discohesive growth pattern was significantly correlated with DFS (HR = 1.899; 95 % CI:1.168-3.087; P = 0.010). However, the filigree micropapillary and discohesive growth pattern did not affect the OS (HR = 2.786; P = 0.317). The competitive risk model revealed that in the stage I cohort, the simultaneous presence of filigree micropapillary and discohesive growth pattern was a risk factor for recurrence and metastasis [sub- distribution HR (SHR) = 1.987; 95 %CI: 1.122-3.518; P = 0.019]. CONCLUSION: Our study verified that the new prognostic stratification system was an effective stratification tool. Filigree micropapillary and discohesive growth pattern may also be risk factors for DFS, postoperative recurrence and metastasis.


Assuntos
Adenocarcinoma de Pulmão , Adenocarcinoma , Neoplasias Pulmonares , Humanos , Neoplasias Pulmonares/patologia , Prognóstico , Adenocarcinoma/patologia , Estadiamento de Neoplasias , Estudos Retrospectivos , Adenocarcinoma de Pulmão/patologia
20.
Talanta ; 253: 123994, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36228556

RESUMO

For the miniature mass spectrometer (MS) with a continuous atmospheric pressure interface (CAPI), the gas in the multi-stage chambers directly affects the performance of the instrument. In this study, a sealed ionization chamber is designed to couple with a conventional mini CAPI-MS. In this configuration, the gas environment in the first ionization chamber can be flexibly changed to regulate the gas conditions throughout the entire instrument. By studying the effect of gas pressure on the performance of the instrument, we found that the instrument shows some unique advantages when the first ionization chamber is under sub-atmospheric pressure (SAP) conditions, such as reducing the load of the vacuum pump by 40%, achieving pump-free injection for gas and liquid samples, and improving the resolution by a factor of 2 without loss of detection sensitivity. Therefore, we propose a new integrated interface called continuous sub-atmospheric pressure interface (CSAPI) for building a miniature ion trap mass spectrometer. The CSAPI specially integrates sample introduction, gas/ions interface, and ionizations, including electrospray ionization (ESI) and secondary electrospray ionization (SESI), making this system more convenient for non-professional handlers to rapidly identify or monitor target analytes in gaseous- and solution-phase samples. We also use this system to study gas composition to further improve performance, being able to achieve a 5-fold sensitivity and 2-fold resolution improvement. At last, some custom applications of the current CSAPI-MS platform are explored and demonstrated, including real-time monitoring of chemical reactions in solution and long-distance sampling and analysis of dried Chinese herbs. In conclusion, this study provides a new approach to constructing a complete, versatile and practical miniature MS instrument.


Assuntos
Pressão Atmosférica
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