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1.
Nanoscale ; 12(20): 11288-11296, 2020 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-32420577

RESUMO

Ternary I-III-VI2 semiconductors usually have narrow band gaps and large absorption coefficients arising from the unique characteristics of their outer-d valence electrons, which are intimately connected with the photothermal conversion properties. AgFeS2 is known as one such material that has the potential to absorb near-infrared light. In this work, we utilized density functional theory (DFT) calculations to evaluate the electronic structure and optical absorption properties of AgFeS2. Strong absorptions were predicted over a wide Vis-NIR region due to the localized 3d electron of Fe atoms, which agree quite well with the UV-Vis-NIR spectra measured by experiment. The as-prepared AgFeS2 nanoparticles were then modified with mPEG-DSPE, an efficient photothermal agent for artery stenosis therapy. Its photothermal conversion effect has been systematically studied, indicating the potential for causing the hyperthermia of macrophages, an essential part of the artery inflammation response. More importantly, both in vitro cell experiments and in vivo mouse-model studies show that the induction of hyperthermia in artery stenosis by using AgFeS2 nanoparticles is safe and effective when injected at a very low concentration. This study provides a novel photothermal platform derived from the inheritability of bandgap structure and also promotes the process of artery inflammation and stenosis therapy.

2.
Carbohydr Polym ; 239: 116246, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32414454

RESUMO

A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC). They were compared systematically with a clinically-used ePTFE graft. S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone. The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties. G-BNC possessed a looser network with the smoothest luminal surface and greater hemocompatibility. Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.

3.
Dalton Trans ; 49(17): 5493-5502, 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32266911

RESUMO

Heat therapy is a promising therapeutic modality for cancer treatment due to the minimum adverse effects of selective local hyperthermia; however, the low heating efficiency of heat therapy under safe conditions is an issue for its bioapplication. Here, we report the synthesis of water-dispersible sulfur doped iron oxides (SDIOs) with different phase structures and the exploration of the relationships between the different SDIOs and their induction heating capacities as a guideline to obtain a photo-magnetic hyperthermia agent. The agent exhibits good biocompatibility, excellent photothermal conversion efficiency (55.8%) and great T2 weighted magnetic resonance imaging (63.7 mM-1 s-1). Significantly, the SDIOs effectively eliminate tumours in a biologically safe AC magnetic field range (H·f = 4.3 < 5.0 × 106 kA m-1 s-1) and with 808 nm laser irradiation at a safe density of 0.33 W cm-2; also, they can be mostly metabolized from the body after one month. The work presented here adopts anion-doped iron oxides to dramatically improve photo-magnetic hyperthermia effects and may enable further exploration in thermotherapeutic research.

4.
J Vasc Surg ; 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32304727

RESUMO

BACKGROUND: The efficacy and safety of combined proximal covered stent-grafting with distal bare stenting are controversial because of the lack of evidence. This systematic review and meta-analysis compares the outcomes of combined proximal covered stent-grafting with those of distal bare stenting (BS group) and proximal covered stent-grafting without distal bare stenting (non-BS group). METHODS: The MEDLINE, EMBASE, and Cochrane Central Register for Controlled Trials databases and key references were searched up to 26 January 2019. Predefined outcomes of interest were mortality, morbidity, and postoperative assessment of aortic remodeling.. We pooled Risk ratios (RRs) of the outcomes of interest using fixed-effects model or random-effects model. RESULTS: Overall, 8 observational studies involving 914 patients were included. There were no significant differences in overall aortic-related mortality (RR, 0.54; CI, 0.24-1.24; P=0.15), the complete thoracic false lumen (FL) thrombosis rate (RR, 1.23; CI, 0.83-1.81; P=0.30) or the complete abdominal FL thrombosis rate (RR, 1.96; CI, 0.68-5.69; P=0.21) between the BS group and the non-BS group. The BS group had a lower rate of partial thoracic FL thrombosis (RR, 0.40; CI, 0.25-0.65; P=0.0002), a lower stent-graft-induced new entry (SINE) rate (RR, 0.08; CI, 0.02-0.41; P=0.003) and a lower reintervention rate (RR, 0.42; CI, 0.26-0.69; P=0.0005). CONCLUSIONS: Combined proximal covered stent-grafting with distal adjunctive bare stenting had the potential to reduce the partial thoracic FL thrombosis rate and the rates of SINE and reintervention but was not associated with lower aortic-related mortality or the complete FL thrombosis rate. Further research with a stricter methodology is needed.

5.
Langmuir ; 36(12): 3096-3103, 2020 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-32178521

RESUMO

The exploration of original computed tomography (CT) imaging contrast agents with enhanced sensitivity and specificity is currently one of the major challenging tasks for precision medicine. Herein, we develop an innovative nanoprobe of dendrimer-stabilized gold nanoparticles (Au DSNs) linked with folic acid (FA) as a targeting ligand and diatrizoic acid (DTA) for specific enhanced tumor CT imaging. In current work, poly(amidoamine) (PAMAM) dendrimers of generation 5 (G5) with amine termini were adopted to entrap Au NPs through a stepwise complexation/reduction method to achieve a higher Au loading than the conventional one-step complexation/reduction method. The prepared [(Au0)120-G5.NH2] NPs were sequentially functionalized with diatrizoic acid (DTA), a typical CT contrast agent based on iodine(I), FA through a poly(ethylene glycol) (PEG) spacer, and carboxylated PEG monomethyl ether (mPEG-COOH), ended with complete acetylation of the leftover dendrimer amine termini. The generated Au DSNs-DTA-FA (Au core diameter = 5.9 nm) were thoroughly characterized. Our data reveal that the Au DSNs-DTA-FA containing Au and I dual radiodense elements are stable, display enhanced CT imaging performance, much higher than the single-radiodense elemental material solely based on Au or I, and possess a quite good cytocompatibility. With the demonstrated FA-rendered specific targeting, the developed Au DSNs-DTA-FA can be employed as a highly efficient nanoprobe for targeted enhanced CT imaging of cancer cells and a subcutaneous tumor model. Overall, the created Au DSNs-DTA-FA may be a powerful nanoprobe for specific enhanced CT imaging of various kinds of FA receptor-expressing tumors or biosystems.

6.
Nanoscale ; 12(7): 4676-4685, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32048702

RESUMO

Platelets play an important role in the early stage of arterial remodeling after injury. Integrin GPIIb/IIIα (αIIbß3) regulates platelet activation in the inside-out and outside-in signaling pathways. The use of tirofiban, an integrin αIIbß3 inhibitor, in clinical therapy is limited by its short in vivo circulation time. Herein, a controlled drug-release system was formulated using CuS@mSiO2-PEG core-shell nanoparticles as near-infrared-triggered nanocarriers to release tirofiban on demand. The nanocarriers possessed good colloidal stability and very high loading efficiency for the integrin αIIbß3 inhibitor (14.5 wt% for tirofiban). Local application of αIIbß3 antagonist-tirofiban on an injured arterial wall inhibited platelet activation, which was accelerated by laser irradiation. Ex vivo platelet-promoted monocyte transmigration trans-well assays revealed decreased monocyte transmigration after platelet activation was inhibited by tirofiban. Two weeks after the wire-induced injury, the intimal area and cellular content were analyzed. The neointimal area was decreased in ApoE-/- mice with CuS@mSiO2-PEG/tirofiban and laser irradiation-promoted tirofiban release, which had limited the neointima formation. The lesions showed a decreased content of macrophages and smooth muscle cells compared with ApoE-/- mice without tirofiban inhibition. Therefore, the action of platelet-integrin αIIbß3 in neointima formation after vascular injury was successfully inhibited in vivo through the controlled release of tirofiban using a near-infrared-triggered nanocarrier, leading to the decrease of early-stage neointima formation. This study also emphasizes the role of platelets in vascular remodeling and provides a new target, namely integrin αIIbß3, for the inhibition of neointimal hyperplasia during vascular inflammation.

7.
J Vasc Surg ; 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31676177

RESUMO

OBJECTIVE: This study investigated the outcomes of emergency in situ laser fenestration (ISLF)-assisted thoracic endovascular aortic repair (TEVAR) for patients with acute Stanford type A aortic dissection unfit for open surgery. METHODS: Twenty patients with acute Stanford type A aortic dissection who were found to be unfit for open surgery, underwent emergency ISLF-assisted TEVAR in our center between March 2016 and December 2018. Anatomic criteria for endovascular repair: coronary artery and aortic valve was not involved, proximal landing zone diameter of 45 mm or less, and proximal landing zone length of 20 mm or greater. Their clinical outcomes were reviewed retrospectively. RESULTS: Twenty patients achieved a procedural success of 100.0%. The 30-day mortality was 10%; two patients died, one of severe pneumonia and the other from cerebral hemorrhage after the operation. Rate of stroke at 30 days was 5%. The average follow-up time was 16 months (range, 3-26 months). One death owing to heart failure occurred at 23 months postoperatively. Kaplan-Meier curve analysis revealed that the 24-month survival rate was 77.1%. Two patients had type Ia endoleaks and one had a type II endoleak. There was no stent graft migration or fenestration-related endoleak and all patients had a thrombosed false lumen in the covered section of the stent grafts. No reintervention, myocardial infarction, transient ischemic attacks, cerebral infarction, or other complications occurred during the follow-up period. CONCLUSIONS: Emergency ISLF-assisted TEVAR is a safe and effective alternative method for treating acute Stanford type A aortic dissection unfit for open surgery.

8.
ACS Appl Mater Interfaces ; 11(44): 41009-41018, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31599564

RESUMO

Inflammatory macrophage (Mφ)-mediated atherosclerosis is a leading cause of mortality and morbidity worldwide. Photothermal therapy (PTT) has been demonstrated as an efficient strategy in killing target cells, and its application in the treatment of inflammation in atherosclerosis is developing. However, the choice of nanomaterials, mechanisms, and side effects are seldom considered. In this study, semiconductor nanomaterials, that is, MoO2 nanoclusters, were synthesized and used for the first time in PTT for inflammatory Mφ-mediated atherosclerosis. Based on cell differential phagocytosis, the optimum amount of MoO2 and treatment time were selected to exert the maximum ablation effect on Mφ and minimal damage on endothelial cells without requiring additional target or selective groups. Moreover, MoO2-based PTT shows an excellent therapeutic effect on atherosclerosis by eliminating Mφ in animal models, with no significant side effects observed. This study explores a new method of nanotechnology and pharmaceutical development by using and optimizing cost-effective metal oxide nanostructures in the treatment of atherosclerosis and motivates further research on minimizing the side effects of related materials.

9.
Nanoscale ; 11(42): 20324-20332, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31641707

RESUMO

Janus heterostructural materials as photothermal agents with enhanced optical conversion capability are promising for artery inflammation treatment by the hyperthermia of macrophages, a primordial part in the artery inflammation response that can deteriorate into atherosclerosis and even break the vessels. Herein, a synthesis route of Janus Ag/Ag2S beads with hydrophilic ligands has been developed with a precise control over concentration, time and surface functionalization. These Ag/Ag2S heterodimers show desirable sizes of around 90 nm in diameter, in which Ag nanocrystals have a diameter of around 25 nm, and they exhibit a photothermal conversion efficiency of up to 50.0% as well as relatively low biotoxicity and good biocompatibility. Importantly, the as-prepared Janus Ag/Ag2S beads with a high biological safety can be effectively swallowed by macrophages and have a remarkable benefit of eliminating these cells from the original state of artery inflammation through the excellent photothermal effect of this material, without causing any further damage to the arteries and major organs in vivo. This study further promotes the development of treatment for vascular inflammation by the photothermal melting of macrophage cells in intima environments.

10.
J Vasc Surg Cases Innov Tech ; 5(3): 379-383, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31453422

RESUMO

Patients with persistent sciatic artery are at high risk for development of limb ischemia, aneurysm formation, and embolism. In this report, we identify a nonclassified left leg persistent sciatic artery in a patient with chronic limb ischemia. Vascular reconstruction was carried out by common iliac-deep femoral artery bypass to restore adequate arterial flow. Our approach to placement of the distal anastomosis on the deep femoral artery instead of on the popliteal artery, which is used in routine practice, may potentially increase treatment efficacy and decrease surgical complications. At 2-year follow-up, the patient remained asymptomatic and in good health.

11.
Nanoscale ; 11(26): 12723, 2019 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-31225567

RESUMO

Correction for 'CuCo2S4 nanocrystals as a nanoplatform for photothermal therapy of arterial inflammation' by Xing Zhang et al., Nanoscale, 2019, 11, 9733-9742.

12.
Nanoscale ; 11(19): 9733-9742, 2019 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-31066405

RESUMO

Ultrasmall CuCo2S4 nanocrystals (NCs) have been demonstrated as an effective agent in the photothermal therapy (PTT) of tumors, but have not been investigated for treatment of arterial inflammation, which is critical in the initiation and development of atherosclerosis (AS), a leading cause of vascular diseases worldwide. In this study, CuCo2S4 NCs were synthesized and used as an efficient PTT nanoplatform for arterial inflammation. In vitro experiments illustrated an effective ablation of inflammatory macrophages by CuCo2S4 incubation combined with the irradiation with an 808 nm near-infrared (NIR) laser. In vivo experiments in an apolipoprotein E knockout (Apo E-/-) mouse model showed that the local injection with CuCo2S4 followed by irradiation with an 808 nm NIR laser notably ablated infiltrating inflammatory macrophages and effectively reduced arterial inflammation and arterial stenosis. This work provides a new strategy for treatment of AS by exploring bimetal sulfides as effective PTT agents.


Assuntos
Estenose das Carótidas/terapia , Cobalto/química , Cobre/química , Raios Infravermelhos , Nanopartículas/química , Animais , Apolipoproteínas E/deficiência , Apolipoproteínas E/genética , Artérias Carótidas/efeitos dos fármacos , Artérias Carótidas/patologia , Estenose das Carótidas/patologia , Sobrevivência Celular/efeitos dos fármacos , Modelos Animais de Doenças , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Nanopartículas/uso terapêutico , Nanopartículas/toxicidade , Fototerapia , Células RAW 264.7
13.
EuroIntervention ; 14(18): e1854-e1860, 2019 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-30719978

RESUMO

AIMS: The aim of the study was to evaluate the feasibility, safety, and effectiveness of in situ diode laser fenestration of thoracic endovascular aortic repair (TEVAR) stent grafts to treat Stanford type A aortic dissection. METHODS AND RESULTS: Fifty-eight patients with acute or subacute Stanford type A aortic dissection treated with in situ diode laser fenestration during TEVAR under cerebral circulation protection with an extracorporeal bypass were reviewed retrospectively. Routine postoperative outcomes were recorded and assessed. Computed tomography angiography (CTA) was performed during the follow-up after 3, 6 and 12 months. Procedural success was achieved in 53 patients (91.4%). The average procedure time was 162±36 minutes. One patient died of pericardial tamponade during intervention, and one died of severe pneumonia after the intervention. Except for two minor strokes, no more fenestration-related complications occurred at 30 days and 12 months after the intervention. CTA imaging demonstrated 100% primary patency for the left subclavian artery and carotid arteries with favourable aortic remodelling after TEVAR during the follow-up. Two patients had a type Ia endoleak and one other a type II endoleak. CONCLUSIONS: In situ diode laser fenestration during TEVAR for type A aortic dissection was found to be feasible, safe, and effective, and may be beneficial as a less invasive approach.


Assuntos
Aneurisma Dissecante , Aneurisma da Aorta Torácica , Implante de Prótese Vascular , Procedimentos Endovasculares , Aortografia , Prótese Vascular , Humanos , Lasers Semicondutores , Estudos Retrospectivos , Stents , Resultado do Tratamento
14.
Ann Vasc Surg ; 58: 151-159, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30769064

RESUMO

BACKGROUND: To evaluate the short-term outcomes of percutaneous mechanical thrombectomy (PMT) in patients with acute lower limb ischemia (ALI) and to analyze the effect of ALI of different etiologies on the limb salvage. MATERIALS AND METHODS: From January 2015 to December 2017, a retrospective analysis was performed on 112 patients (mean age: 66.5 years; 117 limbs in total; 66 limbs in 61 males) with ALI treated with PMT at 2 vascular institutions. Of the 117 limbs, 44 (41 patients) had acute arterial embolism, 36 (34 patients) had acute arterial thrombosis, and 37 (37 patients) had acute stent (31 limbs in 31 patients) or graft (6 limbs in 6 patients) thrombosis. The primary end point was limb salvage rate, and subgroups were analyzed by etiological factors. The secondary end points included patency rates, major bleeding complications, 30-day mortality, and reintervention rates. RESULTS: The 30-day mortality rate was 3.6%. The incidence of major bleeding complications was 2.7%. During the follow-up, the limb salvage rates at 1 year and 2 years were 83.8% and 74.7%, respectively. Subgroup analysis showed that the limb salvage rate in patients with acute arterial embolism was 92.9% at 2 years after PMT, which was higher than that in patients with acute arterial thrombosis (73.3%, P = 0.04, hazard ratio [HR]: 3.6, 95% confidence interval [CI]: 1.1-11.7) and acute stent/graft thrombosis (62.5%, P = 0.01, HR: 4.7, 95% CI: 1.5-13.6). CONCLUSIONS: PMT in patients with ALI is effective in preventing amputations over the 3-year study period with a reasonable safety profile, especially in patients with acute arterial embolism.


Assuntos
Isquemia/cirurgia , Salvamento de Membro , Extremidade Inferior/irrigação sanguínea , Doença Arterial Periférica/cirurgia , Trombectomia/métodos , Doença Aguda , Adulto , Idoso , Idoso de 80 Anos ou mais , Amputação , China , Feminino , Humanos , Incidência , Isquemia/diagnóstico por imagem , Isquemia/mortalidade , Isquemia/fisiopatologia , Masculino , Pessoa de Meia-Idade , Doença Arterial Periférica/diagnóstico por imagem , Doença Arterial Periférica/mortalidade , Doença Arterial Periférica/fisiopatologia , Complicações Pós-Operatórias/mortalidade , Fluxo Sanguíneo Regional , Estudos Retrospectivos , Fatores de Risco , Trombectomia/efeitos adversos , Fatores de Tempo , Resultado do Tratamento , Grau de Desobstrução Vascular
15.
Dalton Trans ; 48(10): 3360-3368, 2019 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-30785146

RESUMO

Although various photothermal therapy (PTT) nanoagents have been developed in recent years, the rational design and easy synthesis of a PTT nanoplatform with improved near-infrared (NIR) absorption have remained challenging. Herein, via a facile one-pot solvothermal strategy, hydrophilic nanosheet-assembled flower-like Fe7S8/Bi2S3 superstructures were fabricated successfully. Such nanoflowers exhibit improved NIR absorption, which is 1.54 times higher than that of pure Bi2S3 nanosheets at a wavelength of 808 nm. Attractively, these nanoflowers could serve as a drug delivery carrier with controlled release under pH/NIR stimuli and display a fascinating chemo-photothermal synergetic therapeutic effect both in vitro and vivo. The resulting nanoflowers may open up a way for the design of other nanoagents with an improved NIR absorption and chemo-photothermal cancer therapy effect.


Assuntos
Antibióticos Antineoplásicos/farmacologia , Bismuto/química , Doxorrubicina/farmacologia , Compostos de Ferro/química , Neoplasias/tratamento farmacológico , Fotoquimioterapia , Sulfetos/química , Enxofre/química , Antibióticos Antineoplásicos/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Doxorrubicina/química , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos , Ensaios de Seleção de Medicamentos Antitumorais , Células HeLa , Humanos , Raios Infravermelhos , Compostos de Ferro/síntese química , Estrutura Molecular , Neoplasias/patologia , Sulfetos/síntese química
16.
Phlebology ; 34(4): 257-265, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30049252

RESUMO

PURPOSE: The aim of the present study was to report the clinical outcomes of endovascular treatment for extensive lower limb deep vein thrombosis with AngioJet rheolytic thrombectomy (ART) plus catheter-directed thrombolysis (CDT) using a contralateral femoral approach. METHODS: A retrospective analysis of consecutive ART+CDT treatments in 38 deep vein thrombosis patients (LET I-III, from September 2014 to March 2016) was performed. RESULTS: The technical success rate was 100%. Complete lysis was achieved in 82% of LET III segments (calf veins), 87% of LET II segments (popliteal-femoral veins), and 90% of LET III segments (iliac veins). The best results were obtained in patients treated within seven days of symptom onset. During follow-up, well-preserved, competent femoral valves were observed in 86% of the patients, and recanalization of LET III, LET II, and LET I segments was achieved in 100%, 94%, and 91% of the patients, respectively. The post-thrombotic syndrome rate was 17% during a mean 20-month follow-up.


Assuntos
Cateterismo Periférico , Procedimentos Endovasculares , Veia Femoral/cirurgia , Extremidade Inferior/cirurgia , Trombólise Mecânica , Trombose Venosa/cirurgia , Doença Aguda , Adulto , Idoso , Cateterismo Periférico/instrumentação , Cateterismo Periférico/métodos , Procedimentos Endovasculares/instrumentação , Procedimentos Endovasculares/métodos , Feminino , Humanos , Extremidade Inferior/fisiopatologia , Masculino , Trombólise Mecânica/instrumentação , Trombólise Mecânica/métodos , Pessoa de Meia-Idade , Estudos Retrospectivos , Trombose Venosa/fisiopatologia
17.
Stem Cell Res Ther ; 9(1): 285, 2018 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-30359296

RESUMO

BACKGROUND: Diabetic peripheral neurovascular diseases (DPNVs) are complex, lacking effective treatment. Autologous/allogeneic transplantation of adipose-derived stem cells (ADSCs) is a promising strategy for DPNVs. Nonetheless, the transplanted ADSCs demonstrate unsatisfying viability, migration, adhesion, and differentiation in vivo, which reduce the treatment efficiency. Netrin-1 secreted as an axon guidance molecule and served as an angiogenic factor, demonstrating its ability in enhancing cell proliferation, migration, adhesion, and neovascularization. METHODS: ADSCs acquired from adipose tissue were modified by Netrin-1 gene (NTN-1) using the adenovirus method (N-ADSCs) and proliferation, migration, adhesion, and apoptosis examined under high-glucose condition. The sciatic denervated mice (db/db) with type 2 diabetes mellitus (T2DM) were transplanted with N-ADSCs and treatment efficiency assessed based on the laser Doppler perfusion index, immunofluorescence, and histopathological assay. Also, the molecular mechanisms underlying Netrin-1-mediated proliferation, migration, adhesion, differentiation, proangiogenic capacity, and apoptosis of ADSCs were explored. RESULTS: N-ADSCs improved the proliferation, migration, and adhesion and inhibited the apoptosis of ADSCs in vitro in the condition of high glucose. The N-ADSCs group demonstrated an elevated laser Doppler perfusion index in the ADSCs and control groups. N-ADSCs analyzed by immunofluorescence and histopathological staining demonstrated the distribution of the cells in the injected limb muscles, indicating chronic ischemia; capillaries and endothelium were formed by differentiation of N-ADSCs. The N-ADSCs group showed a significantly high density of the microvessels than the ADSCs group. The upregulation of AKT/PI3K/eNOS/P-38/NF-κB signaling pathways and secretion of multiple growth factors might explain the positive effects of Netrin-1 on ADSCs. CONCLUSION: The overexpression of Netrin-1 in ADSCs improves proliferation, migration, and treatment effect in type 2 diabetic mice with sciatic denervation, which directs the clinical treatment of patients with DPNVs.


Assuntos
Diabetes Mellitus Experimental/terapia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/metabolismo , Netrina-1/genética , Nervo Isquiático/metabolismo , Adenoviridae/genética , Adenoviridae/metabolismo , Animais , Denervação Autônoma/métodos , Diferenciação Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Diabetes Mellitus Experimental/genética , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/patologia , Diabetes Mellitus Tipo 2/genética , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/patologia , Diabetes Mellitus Tipo 2/terapia , Regulação da Expressão Gênica , Vetores Genéticos/química , Vetores Genéticos/metabolismo , Glucose/farmacologia , Masculino , Células-Tronco Mesenquimais/citologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , NF-kappa B/genética , NF-kappa B/metabolismo , Neovascularização Fisiológica/genética , Netrina-1/metabolismo , Óxido Nítrico Sintase Tipo III/genética , Óxido Nítrico Sintase Tipo III/metabolismo , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Cultura Primária de Células , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Nervo Isquiático/irrigação sanguínea , Nervo Isquiático/lesões , Nervo Isquiático/patologia , Transdução de Sinais , Transfecção
18.
Nanoscale ; 10(37): 17902-17911, 2018 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-30226246

RESUMO

To retain the agents in tumors for cancer diagnosis and therapy, and then to remove them from the body, are key for the clinical applications of ideal inorganic theranostic agents. To meet these needs, we have developed a transformed theranostic platform, employing PVP coated Fe3S4 tetragonal nanosheets (TNSs), which could effectively accumulate in the tumor under magnetic targeting, whilst gradually transforming to small particles (∼5 nm) over three weeks. These were then effectively excreted from the body in normal physiological conditions after exerting their therapeutic effect. The aqueous dispersion of PVP coated Fe3S4 TNSs had an intense near-infrared absorption, excellent photothermal conversion efficiency (64.3%) and great T2 weighted magnetic resonance imaging properties (71.3 mM-1 S-1). In addition, Fe3S4 TNSs could realize a synergistic photothermal therapy (PTT)/chemodynamic therapy (CDT), because the localized heat produced by PTT from the defect-rich structure could enhance the Fenton process by utilizing the overproduced H2O2 in the tumor microenvironment, and in return, the produced ˙OH could inhibit tumor growth and recurrence after PPT. We thus developed a high-efficiency inorganic theranostic platform which was effectively cleared from the body. This will open up a new avenue for the design of inorganic agents for clinical applications in the future.


Assuntos
Ferro/química , Imagem por Ressonância Magnética , Nanoestruturas , Neoplasias Experimentais/tratamento farmacológico , Fotoquimioterapia , Sulfetos/química , Animais , Células HeLa , Humanos , Peróxido de Hidrogênio , Camundongos Endogâmicos BALB C , Camundongos Nus , Nanomedicina Teranóstica , Microambiente Tumoral
19.
Nanoscale ; 10(31): 15116, 2018 08 09.
Artigo em Inglês | MEDLINE | ID: mdl-30046775

RESUMO

Correction for 'Porous cobalt sulfide hollow nanospheres with tunable optical property for magnetic resonance imaging-guided photothermal therapy' by Guoqiang Guan et al., Nanoscale, 2018, DOI: 10.1039/c8nr01926f.

20.
Nanoscale ; 10(29): 14190-14200, 2018 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-30009304

RESUMO

Transition-metal chalcogenides with an imaging element and tunable optical property are strongly desired as ideal high-efficiency photothermal theranostic agents to diagnose and eliminate tumors. Herein, we report on a one-pot solvothermal strategy to synthesize various porous cobalt sulfide hollow nanospheres (PCSH NSs) and elucidate the relation between PCSH NSs and their optical absorption as a guide to obtain optimal photothermal therapy (PTT) agents. After PEG modification, PEG-PCSH NSs show superexcellent photothermal conversion efficiency (∼70.1%) which is higher than that of binary transition-metal chalcogenides materials reported to date. A low dose (100 µL, 25 ppm) could completely ablate tumors under an 808 nm laser power of 0.7 W cm-2, reducing in vivo long-term residual agent content and thus lowering the possibility of side effects. Additionally, they also exhibit excellent biocompatibility, good photostability and utility for magnetic resonance imaging. Our results indicate that PCSH NSs can be considered as an outstanding PTT agent and give guidance towards the design of other photothermal theranostic agents.


Assuntos
Cobalto/química , Imagem por Ressonância Magnética , Nanosferas , Neoplasias Experimentais/terapia , Fototerapia , Nanomedicina Teranóstica , Animais , Células HeLa , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus
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