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1.
Sci Total Environ ; 804: 149680, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34509838

RESUMO

NO3 radicals can clean the atmospheric primary contaminants during the night. However, it can also effect the formation of secondary organic aerosol (SOA) and nitrate, which may worsen air quality. We report field observations of NO3 radicals with a home-made long path differential optical absorption spectroscopy (LP-DOAS) at a rural site in the polluted North China Plain in the summer of 2014. The detection limit (1σ) of NO3 with 3.4 km optical path was 3.4 ppt. The observed mean NO3 mixing ratios were 21 ppt with the maximum value of 104 ppt. The average calculated production rates and steady state lifetime of NO3 were 952 ppt/h and 103 s, respectively. The increase of both PM2.5 (>60 µg/m3) and RH (>60%) would result in an increase of the loss of NO3. The proportion of indirect losses rise with the increase of RH (>50%). The fitting kNO3 ranged from 0.0018 to 0.012 s-1 while γN2O5 was 0.0012 to 0.072. The ratios of direct loss ranged from 20.95% to 90.36% with an average of 56.81% during the campaign.


Assuntos
Poluentes Atmosféricos , Material Particulado , Poluentes Atmosféricos/análise , China , Monitoramento Ambiental , Material Particulado/análise , Análise Espectral
2.
Bioresour Technol ; : 126401, 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34822985

RESUMO

This study explored the regulation of photosystem and central carbon metabolism in cell growth and fucoxanthin accumulation of Isochrysis zhangjiangensis via transcriptome analysis, targeted metabolite measurements, and flux balance analysis. High light promoted biomass accumulation but dramatically decreased fucoxanthin productivity. It suppressed the active photosystem and reduced chlorophyll content, but improved metabolic flux of Calvin-Benson-Bassham and tricarboxylic acid cycle for massive biomass accumulation. The CO2 fixation was largely dependent on mitochondrial energy illustrated by the integrated metabolic tools. At a molecular level, glyceraldehyde-3-phosphate, acetyl-CoA, and pyruvate contents increased at exponential phase under high light, which tended to participate into fatty acid biosynthesis by the up-regulated ACCase. However, high light inhibited most genes involved in fucoxanthin biosynthesis and induced diadinoxanthin cycle to diatoxanthin form. Therefore, constant light at 100 µmol m-2 s-1 balancing biomass concentration and fucoxanthin content provided the highest fucoxanthin productivity at 3.06 mg L-1 d-1.

3.
Front Pharmacol ; 12: 756975, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34776974

RESUMO

Liuweiwuling Tablet (LWWL) is a licensed Chinese patent medicine (approval number: Z20060238) included in the national health insurance for anti-inflammation of chronic HBV infection, whereas its anti-HBV effect remains clarification. The study aimed to clarify its antiviral effect and related mechanisms. HepG2.2.15 cells (wild-type HBV-replicating cells) and HepG2. A64 cells (entecavir-resistant HBV-replicating cells) were used for in vitro test. Hydrodynamic injection-mediated HBV-replicating mouse model was used for in vivo test. Active compounds and related mechanisms for antiviral effect of LWWL were analyzed using network pharmacology and transcriptomics. The inhibition rates of LWWL (0.8 mg/ml) on HBV DNA, HBsAg, and pgRNA were 57.06, 38.55, and 62.49% in HepG2.2.15 cells, and 51.57, 17.57, and 53.88% in HepG2. A64 cells, respectively. LWWL (2 g kg-1 d-1 for 4 weeks)-treated mice had 1.16 log10 IU/mL decrease of serum HBV DNA, and more than 50% decrease of serum HBsAg/HBeAg and hepatic HBsAg/HBcAg. Compared to tenofovir control, LWWL was less effective in suppressing HBV DNA but more effective in suppressing HBV antigens. Thirteen differentially-expressed genes were found in relation to HBV-host interaction and some of them were enriched in interferon (IFN)-ß pathway in LWWL-treated HepG2.2.15 cells. CD3+CD4+ T-cell frequency and serum IFN-γ were significantly increased in LWWL-treated mice compared to LWWL-untreated mice. Among 26 compounds with potential anti-HBV effects that were predicted by network pharmacology, four compounds (quercetin, luteolin, wogonin, and kaempferol) were experimentally confirmed to have antiviral potency. In conclusion, LWWL had potent inhibitory effect on both wild-type and entecavir-resistant HBV, which might be associated with increasing IFN-ß and IFN-γ production.

4.
Sci Rep ; 11(1): 22282, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34782675

RESUMO

Urbanization had a huge impact on the regional ecosystem net primary productivity (NPP). Although the urban heat island (UHI) caused by urbanization has been found to have a certain promoting effect on urban vegetation NPP, the factors on the impact still are not identified. In this study, the impact of urbanization on NPP was divided into direct impact (NPPdir) and indirect impact (NPPind), taking Kunming city as a case study area. Then, the spatial heterogeneity impact of land surface temperature (LST) on NPPind was analyzed based on the geographically weighted regression (GWR) model. The results indicated that NPP, LST, NPPdir and NPPind in 2001, 2009 and 2018 had significant spatial autocorrelation in Kunming based on spatial analytical model. LST had a positive impact on NPPind in the central area of Kunming. The positively correlation areas of LST on NPPind increased by 4.56%, and the NPPind caused by the UHI effect increased by an average of 4.423 gC m-2 from 2009 to 2018. GWR model can reveal significant spatial heterogeneity in the impacts of LST on NPPind. Overall, our findings indicated that LST has a certain role in promoting urban NPP.

5.
Zool Res ; 42(6): 834-844, 2021 11 18.
Artigo em Inglês | MEDLINE | ID: mdl-34766482

RESUMO

Understanding the zoonotic origin and evolution history of SARS-CoV-2 will provide critical insights for alerting and preventing future outbreaks. A significant gap remains for the possible role of pangolins as a reservoir of SARS-CoV-2 related coronaviruses (SC2r-CoVs). Here, we screened SC2r-CoVs in 172 samples from 163 pangolin individuals of four species, and detected positive signals in muscles of four Manis javanica and, for the first time, one M. pentadactyla. Phylogeographic analysis of pangolin mitochondrial DNA traced their origins from Southeast Asia. Using in-solution hybridization capture sequencing, we assembled a partial pangolin SC2r-CoV (pangolin-CoV) genome sequence of 22 895 bp (MP20) from the M. pentadactyla sample. Phylogenetic analyses revealed MP20 was very closely related to pangolin-CoVs that were identified in M. javanica seized by Guangxi Customs. A genetic contribution of bat coronavirus to pangolin-CoVs via recombination was indicated. Our analysis revealed that the genetic diversity of pangolin-CoVs is substantially higher than previously anticipated. Given the potential infectivity of pangolin-CoVs, the high genetic diversity of pangolin-CoVs alerts the ecological risk of zoonotic evolution and transmission of pathogenic SC2r-CoVs.


Assuntos
COVID-19/veterinária , Evolução Molecular , Pangolins/virologia , SARS-CoV-2/genética , Animais , Genoma Viral , Filogenia , RNA Viral/genética
6.
Orthop Surg ; 13(7): 2102-2110, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34599644

RESUMO

OBJECTIVE: To review the outcomes of surgical management in the pediatric patients with extremity chondroblastoma. Especially the risk factors of recurrence and growth disorder. And discuss a potential method to decrease the rate of growth disorder by preventing the premature physeal closure. METHODS: Fifteen girls and twenty-seven boys aged from two to 14 years (mean, 11 years) with histologically proven chondroblastoma, who presented from January 2011 to June 2018 at our Hospital, were retrospectively reviewed. Clinical data, radiographic images, histological findings, treatment, functional outcomes, and local recurrence rate were analyzed. Surgical treatment included complete curettage of the tumor and the walls of the lesion followed by bone grafting, No adjuvant methods were used. Recurrence was defined as a return of symptoms and an expansion radiolucency at the operated site. It was confirmed by the histopathological analysis. When recurrence was diagnosed, the medical data were analyzed to detect the effect of different factors on local recurrence. Functional outcome was measured according to Sailhan's functional criteria, designed to provide a standardized method of assessing pediatric chondroblastoma patient postoperatively. RESULTS: The proximal part of the femur was the most frequently involved site. All the patients had at least 24 months of follow-up; mean duration was 30 months (range, 24-60 months). The local recurrence rate was 9.5%. Three resolved after repeat surgeries without further recurrence, one had a second recurrence and received another more aggressive curettage. Local recurrence of chondroblastoma was associated with age (P < 0.05), while not associated with sex, tumor location, the radiological character of the lesion or the grafting method (P > 0.05). No pulmonary metastasis was noted at latest follow-up. Five patients suffered from premature closure of physis due to physis injury. Thirty-one patients (73.8%) had a good outcome, and all returned to normal unrestricted activities. Six patients (14.3%) had a fair outcome due to occasional pain, asymmetric range of motion, or radiographic joint changes without arthritis. And five patients (11.9%) had a poor outcome because of chronic pain, loss of joint motion impairing normal life activities, or a limb-length discrepancy and limp. CONCLUSIONS: Aggressive curettage and bone grafting resulted in local control and good outcomes in most pediatric patients. Being less than 12 years of age was the risk factor for recurrence. For those growing patients, premature physeal closure was observed after the curettage, interpositional technique with PMMA would be a good choice for prevention.


Assuntos
Neoplasias Ósseas/cirurgia , Transplante Ósseo/métodos , Condroblastoma/cirurgia , Curetagem/métodos , Extremidades/cirurgia , Recidiva Local de Neoplasia/etiologia , Adolescente , Criança , Pré-Escolar , Extremidades/patologia , Feminino , Humanos , Masculino , Estudos Retrospectivos , Fatores de Risco
7.
ACS Omega ; 6(40): 26699-26706, 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34661023

RESUMO

An efficient synthesis of a variety of 2,5-disubstituted 1,3,4-oxadiazole derivatives via a cyclization reaction by photoredox catalysis between aldehydes and hypervalent iodine(III) reagents is described. The reaction proceeds under mild conditions and affords various target compounds in excellent yields. The commercially available aldehydes without preactivation and a simple visible-light-promoted procedure without any catalysts make this strategy an alternative to the conventional methods.

8.
Theranostics ; 11(19): 9705-9720, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34646394

RESUMO

Metastasis is the major cause of high mortality in lung cancer. Exploring the underlying mechanisms of metastasis thus holds promise for identifying new therapeutic strategies that may enhance survival. Methods: We applied quantitative mass spectrometry to compare protein expression profiles between primary and metastatic lung cancer cells whilst investigating metastasis-related molecular features. Results: We discovered that BCAT1, the key enzyme in branched-chain amino acid metabolism, is overexpressed at the protein level in metastatic lung cancer cells, as well as in metastatic tissues from lung cancer patients. Analysis of transcriptomic data available in the TCGA database revealed that increased BCAT1 transcription is associated with poor overall survival of lung cancer patients. In accord with a critical role in metastasis, shRNA-mediated knockdown of BCAT1 expression reduced migration of metastatic cells in vitro and the metastasis of these cells to distal organs in nude mice. Mechanistically, high levels of BCAT1 depleted α-ketoglutarate (α-KG) and promoted expression of SOX2, a transcription factor regulating cancer cell stemness and metastasis. Conclusion: Our findings suggest that BCAT1 plays an important role in promoting lung cancer cell metastasis, and may define a novel pathway to target as an anti-metastatic therapy.

9.
Huan Jing Ke Xue ; 42(10): 4951-4958, 2021 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-34581139

RESUMO

Nitrogen metabolism pathways mediated by microorganisms play an important role in maintaining the structure and functional stability of soil ecosystems. Clarifying the relationships between microbial communities and nitrogen metabolism pathways can expand our understanding of nitrogen metabolism pathways at a microscopic level. However, the horizontal gene transfer of microorganisms means that taxonomy-based methods cannot be easily applied. A growing number of studies have shown that functional traits affect community construction and ecosystem functions. Using methods based on functional traits to study soil microbial communities can, therefore, better characterize nitrogen metabolism pathways. Here, five typical forest soils in China, namely black soil(Harbin, Heilongjiang), dark-brown earth(Changbaishan, Jilin), yellow-brown earth(Wuhan, Hubei), red earth(Fuzhou, Fujian), and humid-thermo ferralitic soil(Ledong, Hainan), were selected to study the traits of nitrogen metabolism pathways using metagenomic technology combined with the trait-based methods. The studied nitrogen metabolism pathways were ammonia assimilation, nitrate dissimilatory reduction, nitrate assimilatory reduction, denitrification, nitrification, nitrogen fixation, and anaerobic ammonia oxidation. The results showed that bacteria dominated the metagenomic library, accounting for 98.02% of all the sequences. Across all domains, the most common pathway was ammonia assimilation. For example, an average of 2830 ammonia assimilation pathway genes were detected for every million annotated bacterial sequences. In comparison, nitrogen fixation and anaerobic ammonia oxidation were the least detected pathways, accounting for 28.3 and 10.7 per million sequences, respectively. Different microorganisms can participate in a same nitrogen metabolism pathway, and the community structure of different soils was variable. The five typical forest soils in China show the same microbial nitrogen metabolism pathway traits; however, the community structure of the microorganisms mediating these processes was found to vary.


Assuntos
Microbiota , Solo , Archaea , China , Florestas , Microbiota/genética , Nitrificação , Nitrogênio , Oxirredução , Microbiologia do Solo
10.
Water Res ; 205: 117667, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34547698

RESUMO

Ferrate(VI) (Fe(VI)) can oxidize individual pollutants, but the pollutant oxidation does not necessarily result in toxicity reduction. Besides, Fe(VI) resultant Fe(III) particles has previously been used to remove heavy metals, but its influence on organic matter and toxicity of wastewater is unknown. This study investigated influence of Fe(VI) on the cytotoxicity and DNA double-strand break (DSB) effects of secondary effluents from wastewater treatment plants to Chinese hamster ovary cells. Adding 5.0 mg/L Fe(VI) as Fe reduced the cytotoxicity and genotoxicity of secondary effluents by 44%-71% and 40%-59%, respectively. The toxicity reduction could be explained by the alleviation of oxidative stress in cells when they were exposed to the Fe(VI)-treated organic matter. Oxidation and coagulation accounted for 60 and 40% of the reductions in cytotoxicity and genotoxicity, demonstrating that both oxidation and coagulation processes can play important roles in reducing toxicity. Molecular weight (MW)-distribution analysis showed that the oxidation process was favored for removing ultraviolet absorbance and fluorescence intensity of organic matter, while the coagulation process removed more dissolved organic carbon (DOC), especially the DOC of fractions with MW < 500 Da. Compared with ferric chloride, the Fe(VI) resultant Fe(III) showed better coagulation performance on organic matter, cytotoxicity and genotoxicity removal, because of the different particle sizes and crystalline structures. This study highlights the benefit of using Fe(VI) in advanced treatment as Fe(VI) reduced the overall toxicity of secondary effluents.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Animais , Células CHO , Cricetinae , Cricetulus , DNA , Quebras de DNA de Cadeia Dupla , Compostos Férricos , Ferro , Oxirredução , Águas Residuárias , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade
11.
Angew Chem Int Ed Engl ; 60(42): 22885-22891, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34351663

RESUMO

Exploring earth-abundant electrocatalysts with excellent activity, robust stability, and multiple functions is crucial for electrolytic hydrogen generation. Porous phosphorized CoNi2 S4 yolk-shell spheres (P-CoNi2 S4 YSSs) were rationally designed and synthesized by a combined hydrothermal sulfidation and gas-phase phosphorization strategy. Benefiting from the strengthened Ni3+ /Ni2+ couple, enhanced electronic conductivity, and hollow structure, the P-CoNi2 S4 YSSs exhibit excellent activity and durability towards hydrogen/oxygen evolution and urea oxidation reactions in alkaline solution, affording low potentials of -0.135 V, 1.512 V, and 1.306 V (versus reversible hydrogen electrode) at 10 mA cm-2 , respectively. Remarkably, when used as the anode and cathode simultaneously, the P-CoNi2 S4 catalyst merely requires a cell voltage of 1.544 V in water splitting and 1.402 V in urea electrolysis to attain 10 mA cm-2 with excellent durability for 100 h, outperforming most of the reported nickel-based sulfides and even noble-metal-based electrocatalysts. This work promotes the application of sulfides in electrochemical hydrogen production and provides a feasible approach for urea-rich wastewater treatment.

12.
Nanotechnology ; 32(46)2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34352738

RESUMO

We numerically demonstrate an ultraviolet graphene ultranarrow absorption in a hybrid graphene-metal structure. The full-width at half maximum of the absorption band being 9 nm in ultraviolet range is achieved based on the coupling of lattice plasmon resonances of the metallic nanostructure to the optical dissipation of graphene. The position, absorbance and linewidth of the hybridized narrow resonant mode tuned by controlling geometrical parameters and materials are systematically investigated. The proposed structure possesses high refractive index sensitivity of 288 nm/RIU and figure of merit of 72, and can also be used to detect small molecules layer of sub-nanometer thickness and refractive index with small changes, providing promising applications in ultra-compact efficient biosensors.

13.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(4): 1136-1140, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34362493

RESUMO

OBJECTIVE: To evaluate the efficacy and safety of CHOP regimen based on doxorubicin hydrochloride liposome in the initial treatment of elderly patients with diffuse large B-cell lymphoma (DLBCL). METHODS: Thirty-one patients with DLBCL treated from January 1, 2012 to December 31, 2019 were analyzed retrospectively, their median age was 83 (71-95) years old, and all of them were in Ⅲ-Ⅳ stage, including 17 cases who had international prognostic index (IPI) ≥ 3. The patients were treated with R-CHOP and CHOP regimens based on doxorubicin hydrochloride liposome. The efficacy and safety were evaluated during and after treatment. RESULTS: A total of 219 chemotherapy cycles and 7 median cycles were performed in 31 patients. The overall response (OR) rate and complete remission (CR) rate was 80.7% (25/31) and 61.3% (19/31), respectively, as well as 2 cases (6.5%) stable, 4 cases (12.9%) progressive. The main toxicities were as follows: the incidence of grade Ⅲ -Ⅳ neutropenia was 29% (9/31); two patients (6.5%) developed degree Ⅰ-Ⅱ cardiac events, which were characterized by new degree Ⅰ atrioventricular block; there were no cardiac events requiring emergency treatment and discontinuation of chemotherapy. The 1-year, 2-year and 3-year overall survival rate was 83.9%, 77.4% and 61.3%, respectively. The 1-year, 2-year and 3-year progression-free survival rate was 77.4%, 64.5% and 61.3%, respectively. CONCLUSION: The chemotherapy regimen based on doxorubicin hydrochloride liposome has better efficacy and higher cardiac safety for elderly patients with DLBCL.


Assuntos
Lipossomos , Linfoma Difuso de Grandes Células B , Idoso , Idoso de 80 Anos ou mais , Protocolos de Quimioterapia Combinada Antineoplásica , Ciclofosfamida/uso terapêutico , Doxorrubicina/uso terapêutico , Humanos , Lipossomos/uso terapêutico , Linfoma Difuso de Grandes Células B/tratamento farmacológico , Prednisolona , Prednisona/uso terapêutico , Estudos Retrospectivos , Rituximab/uso terapêutico , Vincristina/uso terapêutico
14.
Sci Total Environ ; 794: 148765, 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34225149

RESUMO

Nanoparticles released into the environment are attracting increasing concern because of their potential toxic effects. Conventional methods for assessing the toxicity of nanoparticles are usually confined to cultivable cells, but not applicable to viable but non-culturable (VBNC) cells. However, it remains unknown whether silver nanoparticles (AgNPs), a typical antimicrobial agent, could induce bacteria into a VBNC state in natural environments. In this work, the viability of E. coli, an indicator bacterium widely used for assessing the antibacterial activity of AgNPs, was examined through coupling plate counting, fluorescence staining and adenosine triphosphate (ATP) production. AgNPs were found to have a considerable antibacterial ability, which resulted in less than 0.0004% of culturable cells on plates. However, more than 80% of the cells still maintained their cell membrane integrity under the stress of 80 mg/L AgNPs. Meanwhile, the residue of ATP production (0.6%) was 1500 times higher than that of the culturable cells (< 0.0004%). These results clearly demonstrate that when exposed to AgNPs, most of cells fell into a VBNC state, instead of dying. Environmental factors, e.g., Cl- and illumination, which could change the dissolution, hydrophilicity and zeta potential of AgNPs, eventually influenced the culturability of E. coli. Inhibition of dissolved Ag+ and reactive oxygen species was found to facilitate the mitigation of the strain into a VBNC state. Our findings suggest the necessity of re-evaluating the environmental effects and antibacterial activities of AgNPs.


Assuntos
Escherichia coli , Nanopartículas Metálicas , Antibacterianos/toxicidade , Sobrevivência Celular , Nanopartículas Metálicas/toxicidade , Prata/toxicidade
15.
Pharmgenomics Pers Med ; 14: 823-837, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34285551

RESUMO

Background: Considered as one of the major reasons of sudden cardiac death, hypertrophic cardiomyopathy (HCM) is a common inherited cardiovascular disease. However, effective treatment for HCM is still lacking. Identification of hub gene may be a powerful tool for discovering potential therapeutic targets and candidate biomarkers. Methods: We analysed three gene expression datasets for HCM from the Gene Expression Omnibus. Two of them were merged by "sva" package. The merged dataset was used for analysis while the other dataset was used for validation. Following this, a weighted gene coexpression network analysis (WGCNA) was performed, and the key module most related to HCM was identified. Based on the intramodular connectivity, we identified the potential hub genes. Then, a receiver operating characteristic curve analysis was performed to verify the diagnostic values of hub genes. Finally, we validated changes of hub genes, for genetic transcription and protein expression levels, in datasets of HCM patients and myocardium of transverse aortic constriction (TAC) mice. Results: In the merged dataset, a total of 455 differentially expressed genes (DEGs) were identified from normal and hypertrophic myocardium. In WGCNA, the blue module was identified as the key module and the genes in this module showed a high positive correlation with HCM. Functional enrichment analysis of DEGs and key module revealed that the extracellular matrix, fibrosis, and neurohormone pathways played important roles in HCM. FRZB, COL14A1, CRISPLD1, LUM, and sFRP4 were identified as hub genes in the key module. These genes showed a good predictive value for HCM and were significantly up-regulated in HCM patients and TAC mice. We also found protein expression of LUM and sFRP4 increased in myocardium of TAC mice. Conclusion: This study revealed that five hub genes are involved in the occurrence and development of HCM, and they are potentially to be used as therapeutic targets and biomarkers for HCM.

16.
Artigo em Inglês | MEDLINE | ID: mdl-34266630

RESUMO

In order to assess the health risk of low-dose radiation to radiation professionals, monitoring is performed through chromosomal aberration analysis and micronuclei (MN) analysis. MN formation has drawbacks for monitoring in the low-dose range. Nucleoplasmic bridge (NPB) analysis, with a lower background level, has good dose-response relationships at both high and relatively low dose ranges. Dicentric and ring chromosomes were analyzed in 199 medical radiation professionals, and NPB/MN yields were analyzed in 205 radiation professionals. The effects of sex, age of donor, types of work, and length of service on these cytogenetic endpoints were also analyzed. The yields of the three cytogenetic endpoints were significantly higher in radiation professionals versus controls. Frequencies of dicentric plus ring chromosomes were affected by length of service. NPB frequencies were influenced by type of work and length of service. MN yields were affected not only by types of work and length of service but also by donor sex and age. In conclusion, dicentric plus ring chromosomes, NPB, and MN can be induced by low-dose radiation in radiation professionals. NPB is a potential biomarker to assess the health risk of occupational low-dose radiation exposure.


Assuntos
Raios gama/efeitos adversos , Linfócitos/efeitos da radiação , Exposição Ocupacional/efeitos adversos , Lesões por Radiação/genética , Adulto , Idoso , Núcleo Celular/efeitos da radiação , Aberrações Cromossômicas/efeitos da radiação , Análise Citogenética/métodos , Citogenética/métodos , Dano ao DNA/efeitos da radiação , Feminino , Humanos , Masculino , Micronúcleos com Defeito Cromossômico/efeitos da radiação , Testes para Micronúcleos/métodos , Pessoa de Meia-Idade , Radiação Ionizante , Adulto Jovem
17.
Angew Chem Int Ed Engl ; 60(41): 22189-22194, 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34313363

RESUMO

Prussian blue analogs (PBAs) are considered as reliable and promising cathode materials for aqueous Zn-ion batteries (AZIBs), but they suffer from low capacity and poor cycling stability due to insufficient active sites and structural damage caused by the ion insertion/extraction processes. Herein, a template-engaged ion exchange approach has been developed for the synthesis of Co-substituted Mn-rich PBA hollow spheres (CoMn-PBA HSs) as cathode materials for AZIBs. Benefiting from the multiple advantageous features including hollow structure, abundant active sites, fast Zn2+ ion diffusion, and partial Co substitution, the CoMn-PBA HSs electrode shows efficient zinc ion storage properties in terms of high capacity, decent rate capability and prolonged cycle life.

18.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(3): 975-982, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34105503

RESUMO

OBJECTIVE: To analyze and predict the effect of coronavirus infection on hematopoietic system and potential intervention drugs, and explore their significance for coronavirus disease 2019 (COVID-19). METHODS: The gene expression omnibus (GEO) database was used to screen the whole genome expression data related with coronavirus infection. The R language package was used for differential expression analysis and KEGG/GO enrichment analysis. The core genes were screened by PPI network analysis using STRING online analysis website. Then the self-developed apparent precision therapy prediction platform (EpiMed) was used to analyze diseases, drugs and related target genes. RESULTS: A database in accordance with the criteria was found, which was derived from SARS coronavirus. A total of 3606 differential genes were screened, including 2148 expression up-regulated genes and 1458 expression down-regulated genes. GO enrichment mainly related with viral infection, hematopoietic regulation, cell chemotaxis, platelet granule content secretion, immune activation, acute inflammation, etc. KEGG enrichment mainly related with hematopoietic function, coagulation cascade reaction, acute inflammation, immune reaction, etc. Ten core genes such as PTPRC, ICAM1, TIMP1, CXCR5, IL-1B, MYC, CR2, FSTL1, SOX1 and COL3A1 were screened by protein interaction network analysis. Ten drugs with potential intervention effects, including glucocorticoid, TNF-α inhibitor, salvia miltiorrhiza, sirolimus, licorice, red peony, famciclovir, cyclosporine A, houttuynia cordata, fluvastatin, etc. were screened by EpiMed plotform. CONCLUSION: SARS coronavirus infection can affect the hematopoietic system by changing the expression of a series of genes. The potential intervention drugs screened on these grounds are of useful reference significance for the basic and clinical research of COVID-19.


Assuntos
COVID-19 , Proteínas Relacionadas à Folistatina , Sistema Hematopoético , Preparações Farmacêuticas , Biologia Computacional , Humanos , SARS-CoV-2
19.
Glia ; 69(10): 2404-2418, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34110044

RESUMO

Methamphetamine (METH) is a common abused drug. METH-triggered glutamate (Glu) levels in dorsal CA1 (dCA1) could partially explain the etiology of METH-caused abnormal memory, but the synaptic mechanism remains unclear. Here, we found that METH withdrawal disrupted spatial memory in mice, accompanied by the increases in Glu levels and postsynaptic neuronal activities at dCA1 synapses. METH withdrawal weakened the capacity of Glu clearance in astrocytes, as indicated by increasing the A1-like astrocytes and phosphorylated signal transducer and activator of transcription 3 (p-STAT3), decreasing the Glu transporter 1(GLT-1, also known as EAAT2 or SLC1A2), Glu-aspartate-transporter (GLAST also known as EAAT1 or SLC1A3) and astrocytic glutamine synthase (GS), but failed to affect the presynaptic Glu release from dCA3 within dCA1. Moreover, we identified that in vitro A1-like astrocytes exhibited an increased STAT3 activation and the impaired capacity of Glu clearance. Most importantly, selective knockdown of astrocytic STAT3 in vivo in dCA1 restored the astrocytic capacity of Glu clearance, normalized Glu levels at dCA1 synapses, and finally rescued METH withdrawal-disrupted spatial memory in mice. Thus, astrocytic Glu clearance system, especially STAT3, serves as a novel target for future therapies against METH neurotoxicity.

20.
Angew Chem Int Ed Engl ; 60(35): 19068-19073, 2021 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-34137497

RESUMO

Highly efficient electrocatalysts are essential for the production of green hydrogen from water electrolysis. Herein, a metal-organic framework-assisted pyrolysis-replacement-reorganization approach is developed to obtain ultrafine Pt-Co alloy nanoparticles (sub-10 nm) attached on the inner and outer shells of porous nitrogen-doped carbon nanotubes (NCNT) with closed ends. During the thermal reorganization, the migration of Pt-Co nano-alloys to both surfaces ensures the maximized exposure of active sites while maintaining the robust attachment to the porous carbon matrix. Density functional theory calculations suggest a nearly thermodynamically-neutral free energy of adsorption for hydrogen intermediates and diversified active sites induced by alloying, thus resulting in a great promotion in intrinsic activity towards the hydrogen evolution reaction (HER). Benefiting from the delicate structural design and compositional modulation, the optimized Pt3 Co@NCNT electrocatalyst manifests outstanding HER activity and superior stability in both acidic and alkaline media.

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