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1.
FASEB J ; 35(9): e21667, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34405442

RESUMO

Long noncoding RNAs (lncRNAs) are central regulators of the inflammatory response and play an important role in inflammatory diseases. PINT has been reported to be involved in embryonic development and tumorigenesis. However, the potential functions of PINT in the innate immune system are largely unknown. Here, we revealed the transcriptional regulation of inflammatory genes by PINT, whose expression is primarily dependent on the NF-κB signaling pathway in human and mouse macrophage and intestinal epithelial cell lines. Functionally, PINT selectively regulates the expression of TNF-α in basal and LPS-stimulated cells. Mechanistically, PINT acts as a modular scaffold of p65 and EZH2 to coordinate their localization and specify their binding to the target genes. Further, a high expression level of PINT was detected in intestinal mucosal tissues from patients with ulcerative colitis (UC). Together, these findings demonstrate that PINT acts as an activator of inflammatory responses, highlighting the importance of this lncRNA as a potential therapeutic target in infectious diseases and inflammatory diseases.


Assuntos
Proteína Potenciadora do Homólogo 2 de Zeste/metabolismo , Regulação da Expressão Gênica , RNA Longo não Codificante/genética , Fator de Transcrição RelA/metabolismo , Transcrição Genética , Fator de Necrose Tumoral alfa/genética , Animais , Linhagem Celular , Colite Ulcerativa/genética , Colite Ulcerativa/patologia , Citocinas/genética , Humanos , Doenças Inflamatórias Intestinais/genética , Doenças Inflamatórias Intestinais/patologia , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Macrófagos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Regiões Promotoras Genéticas/genética , Ligação Proteica , Transcrição Genética/genética
2.
BMC Geriatr ; 21(1): 452, 2021 08 04.
Artigo em Inglês | MEDLINE | ID: mdl-34348649

RESUMO

BACKGROUND: Aging is a natural life process and with an aging population, age-related diseases (e.g. type 2 diabetes mellitus (T2DM), atherosclerosis-based cardiovascular diseases) are the primary mortality cause in older adults. Telomerase is often used as an aging biomarker. Detection and characterization of novel biomarkers can help in a more specific and sensitive identification of a person's aging status. Also, this could help in age-related diseases early prevent, ultimately prolonging the population's life span. Sirtuin 6 (Sirt6) - a member of the Sirtuins NAD+-dependent histone deacetylases family - is mainly intracellularly expressed, and is reported to be involved in the regulation of aging and aging-related diseases. Whether serum Sirt6 is correlated with aging and could be used as an aging biomarker is unknown. In the present study, we aimed to investigate the age-related Sirt6 changes in the serum of human adults. METHODS: Participants were divided into three groups according to age: 20-30 years (Young); 45-55 years (Middle-aged); and ≥ 70 years (Old). The Sirt6 and telomerase serum concentrations were determined by ELISA. The Sirt6 and human telomerase reverse transcriptase (hTERT) expression in vessels from amputated human lower limbs were analyzed using real-time quantitative PCR (RT-qPCR) and immunohistochemical staining. The relationships between variables were evaluated by Pearson correlation analysis. RESULTS: The Sirt6 and telomerase serum levels reduced with an increase in age. A similar tendency was observed for Sirt6 and hTERT in the vessel. Serum levels of Sirt6 were higher in females compared with males. Pearson's regression analysis revealed that the Sirt6 serum level positively correlated with telomerase (r = 0.5743) and both were significantly negatively correlated with age (r = - 0.5830 and r = - 0.5993, respectively). CONCLUSIONS: We reported a negative correlation between serum Sirt6 concentration and aging in human beings. Therefore, the Sirt6 serum level is a potential sex-specific aging marker.


Assuntos
Envelhecimento , Diabetes Mellitus Tipo 2 , Sirtuínas , Adulto , Idoso , Biomarcadores , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Sirtuínas/sangue , Adulto Jovem
3.
Infect Dis Ther ; 9(4): 981-1002, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33113119

RESUMO

INTRODUCTION: Diabetes is one of the most common comorbidities of COVID-19. We aimed to conduct a multidimensional analysis of risk factors associated with the severity and mortality of patients with COVID-19 and diabetes. METHODS: In this retrospective study involving 1443 patients with COVID-19, we analyzed the clinical and laboratory characteristics and risk factors associated with disease severity in patients with COVID-19 with and without diabetes. Binary logistic regression analyses were performed to identify the risk factors associated with mortality in patients with COVID-19 and diabetes. The 84-day survival duration for critical patients with COVID-19 and diabetes who had different levels of leukocytes and neutrophils, or treated with immunoglobulin or not, was conducted using Kaplan-Meier survival curves. RESULTS: Of the 1443 patients with COVID-19, 256 (17.7%) had diabetes, had a median age of 66.0 [IQR 58.0-73.8] years, and were more likely to develop severe (41.8% vs. 35.6%) and critical disease (34.0% vs. 14.9%), followed by higher mortality (21.1% vs. 7.0%), than those without diabetes. Higher levels of leukocytes (> 5.37 × 109/L), older age, and comorbid cerebrovascular disease and chronic renal disease independently contributed to in-hospital death of patients with COVID-19 and diabetes. Leukocytes > 5.37 × 109/L and the application of immunoglobulin were associated with shorter survival duration and lower mortality, respectively, in critical patients with COVID-19 and diabetes. CONCLUSIONS: More attention should be paid to patients with COVID-19 and diabetes, especially when they have high leukocyte counts (> 5.37 × 109/L). Timely and adequate intravenous immunoglobulin (IVIG) use may reduce the mortality of critical patients with COVID-19 and diabetes.

4.
Oxid Med Cell Longev ; 2020: 9595036, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32685103

RESUMO

Subendothelial retention of apolipoprotein B100-containing lipoprotein, such as low-density lipoprotein (LDL), is the initial step of atherogenesis. Activation of autophagy exhibits beneficial effects for the treatment of atherosclerosis. In our previous study, we demonstrated that hyperglycemia suppressed autophagic degradation of caveolin-1, which in turn resulted in acceleration of caveolae-mediated LDL transcytosis across endothelial cells and lipid retention. Therefore, targeting the crossed pathway in autophagy activation and LDL transcytosis interruption may be a promising antiatherosclerotic strategy. In metabolic diseases, including atherosclerosis, salidroside, a phenylpropanoid glycoside compound (3,5-dimethoxyphenyl) methyl-ß-glucopyranoside), is the most important compound responsible for the therapeutic activities of Rhodiola. However, whether salidroside suppresses LDL transcytosis to alleviate atherosclerosis has not yet been elucidated. In the present study, we demonstrated that salidroside significantly decreased LDL transcytosis across endothelial cells. Salidroside-induced effects were dramatically blocked by AMPK (adenosine monophosphate-activated protein kinase) inhibitor (compound c, AMPKα siRNA) and by overexpression of exogenous tyrosine-phosphorylated caveolin-1 using transfected cells with phosphomimicking caveolin-1 on tyrosine 14 mutant plasmids (Y14D). Furthermore, we observed that salidroside promoted autophagosome formation via activating AMPK. Meanwhile, the interaction between caveolin-1 and LC3B-II, as well as the interaction between active Src (indicated by the phosphorylation of Src on tyrosine 416) and LC3B-II, was significantly increased, upon stimulation with salidroside. In addition, both bafilomycin A1 (a lysosome inhibitor) and an AMPK inhibitor (compound c) markedly prevented salidroside-induced autophagic degradation of p-Src and caveolin-1. Moreover, the phosphorylation of caveolin-1 on tyrosine 14 was disrupted due to the downregulation of p-Src and caveolin-1, thereby directly decreasing LDL transcytosis by attenuating the number of caveolae on the cell membrane and by preventing caveolae-mediated LDL endocytosis released from the cell membrane. In ApoE-/- mice, salidroside significantly delayed the formation of atherosclerotic lesions. Meanwhile, a significant increase in LC3B, accompanied by attenuated accumulation of the autophagy substrate SQSTM1, was observed in aortic endothelium of ApoE-/- mice. Taken together, our findings demonstrated that salidroside protected against atherosclerosis by inhibiting LDL transcytosis through enhancing the autophagic degradation of active Src and caveolin-1.


Assuntos
Caveolina 1/metabolismo , Células Endoteliais/metabolismo , Glucosídeos/uso terapêutico , Lipoproteínas LDL/metabolismo , Fenóis/uso terapêutico , Quinases da Família src/metabolismo , Animais , Autofagia , Humanos , Camundongos , Transcitose , Transfecção
5.
Sci Total Environ ; 737: 139762, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32521363

RESUMO

Coal-dominated winter heating practices in China are largely accepted to be a leading cause of winter haze in the region though the amount of coal for heating is actually much lower than for power generation or industrial process. However, little is known about how the total rural coal weight in a region could be attributed to real time (e.g., daily) patterns, limiting the understanding of dynamic impacts of coal emissions and the adoption of timely measures against predicted haze. Considering that winter heating essentially protects against cold temperatures, coal burning strength may be related to the temperatures that people experience. A field study was organized to test the validity of this hypothesis. A system was designed to continuously monitor every instance of coal addition, and coal consumption on any given day for a whole village (WDAY) was calculated by summating all the additions. Meanwhile, a new term, composite temperature (TCOM), which incorporates a few weather-related elements, was introduced to represent cold temperatures that individuals experience. It was found that WDAY and TCOM presented opposite variations, and a negative linear correlation was observed (WDAY = -0.75TCOM + 11.86, R2 = 0.75), revealing the feasibility of estimating coal consumption on a certain day (WDAY) based on weather data (TCOM) for a given village. An extensive form of the algorithm for any area of interest (e.g., a district, city, or province) can be expressed as WDAY = (-0.75TCOM + 11.86)‧NH/834, where NH denotes the number of households in a region. This algorithm reflects the essence of winter heating (to resist cold temperatures), and therefore its logic is highly likely to be useful for any countries of the world regardless of what forms of energy used (coal or other energy forms) provided the energy involved is unexceptionally used for winter heating, though there may be some uncertainties in estimated coal consumption due to multiple factors.

6.
J Int Med Res ; 48(6): 300060520932053, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32552092

RESUMO

OBJECTIVE: Our objective was to compare the effectiveness of nonbiological artificial liver (NBAL) support, particularly short-term (28-day) survival rates, in patients who underwent treatment using double plasma molecular adsorption system (DPMAS), plasma exchange (PE), or combined PE+DPMAS, in addition to comprehensive physical treatment for different stages of acute-on-chronic liver failure (ACLF). METHODS: We retrospectively reviewed clinical data of 135 patients with ACLF who received NBAL treatment between November 2015 and February 2019. The patients were categorized into PE, DPMAS, and PE+DPMAS groups. Short-term effectiveness of treatment was assessed and compared based on selected clinical findings, laboratory parameters, and liver function markers. RESULTS: Coagulation function improved significantly in all groups after treatment. In the PE and PE+DPMAS groups, prothrombin time decreased to different degrees, whereas plasma thromboplastin antecedent increased significantly after treatment. White blood cell counts increased and platelet counts decreased in all groups after treatment. The model for end-stage liver disease score, Child-Pugh grade, systematic inflammatory syndrome score, and sepsis-related organ failure score decreased in all three groups after treatment. CONCLUSIONS: PE, DPMAS, and PE+DPMAS improved disease indicators in all patients with ACLF. The combined treatment improved the short-term effectiveness of treatment, especially in patients with mild ACLF.


Assuntos
Insuficiência Hepática Crônica Agudizada/terapia , Fígado Artificial/efeitos adversos , Troca Plasmática/métodos , Insuficiência Hepática Crônica Agudizada/sangue , Insuficiência Hepática Crônica Agudizada/mortalidade , Adsorção/fisiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Bilirrubina/sangue , Coagulação Sanguínea/fisiologia , Feminino , Humanos , Fígado , Masculino , Pessoa de Meia-Idade , Plasma/metabolismo , Tempo de Protrombina/métodos , Estudos Retrospectivos , Taxa de Sobrevida
7.
Huan Jing Ke Xue ; 40(10): 4404-4411, 2019 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854807

RESUMO

Intermediate-volatility organic compounds (IVOCs) are important precursors of secondary organic aerosols that have received much recent attention; however, there is a lack of emission data for IVOCs from combustion sources, especially for residential solid fuel combustion. In this study, two bituminous coals with different geological maturity (XZ and LW) and two biomass types (rice stalk and pinewood) were tested at seven temperature points (300-900℃ with an interval of 100℃) using a quartz tube furnace together with a dilution sampling system. The results showed that the averaged emission factor for IVOCs from biomass combustion[(483±182) mg·kg-1] was 2.5 times higher than that of bituminous coal combustion (190±108) mg·kg-1. The remaining unresolved complex mixture (UCM) dominated the IVOC content for both biomass and coal combustions (81%±11% and 68%±6%, respectively). The fraction of normal and branched alkanes for biomass combustions was lower than for coal combustion, while the fraction of aromatics was higher. The effect of temperature on the emission of IVOCs and their composition was significantly different between the two types of fuel. The emission factor for IVOCs from coal (using LW as an example) at 500℃[(340±113) mg·kg-1] was 5.4 times higher than at 900℃[(63±15) mg·kg-1], while two comparable peaks are observed at 400-500℃ and 800-900℃. As the combustion temperature increased, the proportion of aromatic hydrocarbons in pinewood IVOCs increased from 1% to 29%, while the remaining UCM decreased from 92% to 61%. However, there was little variation for coal combustion. The effects of fuel type and temperature on the emission of IVOCs were further explained with respect to the modified combustion efficiency (MCE).

8.
J Immunol ; 203(6): 1548-1559, 2019 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-31383742

RESUMO

Long noncoding RNAs are important regulators of gene expression in innate immune responses. Antisense IL-7 (IL-7-AS) is a newly discovered long noncoding RNA in human and mouse that has been reported to regulate the expression of IL-6. However, the potential function of IL-7-AS in innate immune system is not fully understood. In this study, we found that the expression of IL-7-AS is primarily dependent on the NF-κB and MAPK signaling pathways in macrophages and intestinal epithelial cells. Functionally, IL-7-AS promotes the expression of several inflammatory genes, including CCL2, CCL5, CCL7, and IL-6, in cells in response to LPS. Specifically, IL-7-AS physically interacts with p300 to regulate histone acetylation levels around the promoter regions of these gene loci. Moreover, IL-7-AS and p300 complex modulate the assembly of SWI/SNF complex to the promoters. IL-7-AS regulates chemotaxis activity of monocytes to intestine epithelial cells with involvement of CCL2. Therefore, our data indicate a new promoting role for NF-κB/MAPK-responsive IL-7-AS in the transcriptional regulation of inflammatory genes in the innate immune system although modulation of histone acetylation around the promoters of related genes.


Assuntos
Montagem e Desmontagem da Cromatina/genética , Histonas/genética , Inflamação/genética , Interleucina-7/genética , RNA Longo não Codificante/genética , Transcrição Genética/genética , Acetilação , Animais , Linhagem Celular , Linhagem Celular Tumoral , Feminino , Células HEK293 , Humanos , Imunidade Inata/genética , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Regiões Promotoras Genéticas/genética , Células RAW 264.7 , Transdução de Sinais/genética , Células U937
9.
Zootaxa ; 4496(1): 382-394, 2018 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-30313708

RESUMO

The Changbai Mountain in northeast China harbours a species-rich enchytraeid fauna, and ca. 50 species of 11 genera have been reported so far from its well-preserved ecosystems. Herein, we add two new species of Mesenchytraeus into this enchytraeid list. Both members belong to the lineage of Mesenchytraeus with enlarged ventral chaetae. Mesenchytraeus spermatoglomeratus sp. nov. is characterized by the presence of enlarged chaetae in V-VI and XI (2 per bundle), five pairs of preclitellar nephridia (VI/VII-X/XI), sperm sacs containing curly ball-shaped sperm bundles, spermathecae without diverticula, atrium with 4-6 developed prostate glands and long vasa deferentia backward to XV-XXII. Mesenchytraeus liberothecus sp. nov. is diagnosed by the presence of enlarged chaetae in V-VI (2 per bundle), five pairs of preclitellar nephridia (VI/VII-X/XI), asymmetrical paired sperm sacs containing flame-shaped sperm bundles, spermathecae with two small diverticula but free from oesophagus, atrium with 4-5 large prostate glands and short vasa deterentia confined to XI and XII. The major differences between the new species and their congeners are discussed. We also add them into the key of this enlarged chaetae lineage.


Assuntos
Ecossistema , Oligoquetos , Animais , China , Masculino
10.
Zootaxa ; 4496(1): 395-410, 2018 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-30313709

RESUMO

The biodiversity in the Tibetan region is still poorly known, particularly for its soil organisms. Here, we describe eleven enchytraeid species based on several taxonomical surveys in 2015-2016, from some soil localities in south region of the Tibet, China. All are firstly recorded in this region; seven species (Fridericia ratzeli sensu stricto, F. peregrinabunda, F. cusanica, F. bretscheri, Buchholzia sp., Enchytraeus buchholzi and Henlea sp.) are new records for China.


Assuntos
Oligoquetos , Animais , Biodiversidade , China , Solo , Tibet
11.
J Immunol ; 199(10): 3571-3582, 2017 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-28993514

RESUMO

Long noncoding RNAs, a newly identified class of noncoding RNAs, are important regulators of gene expression in innate immunity. We report in this study that the transcription of FIRRE, a conserved long noncoding RNA between humans and mice, is controlled by NF-κB signaling in macrophages and intestinal epithelial cells. Functionally, FIRRE appears to positively regulate the expression of several inflammatory genes in macrophages or intestinal epithelial cells in response to LPS stimulation via posttranscriptional mechanisms. Specifically, FIRRE physically interacts with heterogeneous nuclear ribonucleoproteins U, regulating the stability of mRNAs of selected inflammatory genes through targeting the AU-rich elements of their mRNAs in cells following LPS stimulation. Therefore, our data indicate a new regulatory role for NF-κB-responsive FIRRE in the posttranscriptional regulation of inflammatory genes in the innate immune system.


Assuntos
Sequência Conservada/genética , Inflamação/genética , Mucosa Intestinal/imunologia , Macrófagos/imunologia , NF-kappa B/metabolismo , RNA Longo não Codificante/genética , Elementos de Resposta/genética , Animais , Regulação da Expressão Gênica , Ribonucleoproteínas Nucleares Heterogêneas/metabolismo , Humanos , Imunidade Inata , Camundongos , RNA Longo não Codificante/metabolismo , RNA Interferente Pequeno/genética , Células U937
12.
Sci Rep ; 5: 17590, 2015 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-26631623

RESUMO

Aberrant cellular responses to pro-inflammatory cytokines, such as IFN-γ, are pathogenic features in many chronic inflammatory diseases. A variety of feedback regulatory pathways have evolved to prevent an inappropriate cellular reaction to these pro-inflammatory cytokines. CX3CL1 is a unique chemokine and plays an important role in chronic liver diseases. We report here that IFN-γ stimulation induces a transient CX3CL1 production in liver epithelial cells (i.e., hepatocytes and biliary epithelial cells). This transient CX3CL1 production is accompanied with a destabilization of CX3CL1 mRNA associated with the induction of the KH-type splicing regulatory protein (KSRP). IFN-γ treatment of liver epithelial cells decreases expression level of miR-27b, a miRNA that targets the 3' untranslated region of KSRP mRNA resulting in translational suppression. Induction of KSRP following IFN-γ stimulation depends on the downregulation of miR-27b. Functional manipulation of KSRP or miR-27b caused reciprocal alterations in CX3CL1 mRNA stability in liver epithelial cells. Moreover, transfection of miR-27b precursor influences CX3CL1-associated chemotaxis effects of biliary epithelial cells to Jurkat T cells. These findings suggest that miR-27b-mediated post-transcriptional suppression controls the expression of KSRP in liver epithelial cells, and upregulation of KSRP destabilizes CX3CL1 mRNA, providing fine-tuning of cellular inflammatory reactions in response to IFN-γ stimulation.


Assuntos
Quimiocina CX3CL1/genética , Interferon gama/metabolismo , Fígado/citologia , MicroRNAs/genética , Proteínas de Ligação a RNA/metabolismo , Transativadores/metabolismo , Animais , Quimiocina CX3CL1/metabolismo , Quimiotaxia/genética , Células Epiteliais/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Interferon gama/farmacologia , Fígado/efeitos dos fármacos , Masculino , Camundongos Endogâmicos C57BL , MicroRNAs/metabolismo , Estabilidade de RNA/efeitos dos fármacos , Proteínas de Ligação a RNA/genética , Elementos de Resposta/genética , Transativadores/genética , Regulação para Cima/efeitos dos fármacos
13.
Biochemistry (Mosc) ; 77(8): 941-5, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22860917

RESUMO

Potassium channel tetramerization domain containing 1 (KCTD1) contains a BTB domain, which can facilitate protein-protein interactions that may be involved in the regulation of signaling pathways. Here we describe an expression and purification system that can provide a significant amount of recombinant KCTD1 from Escherichia coli. The cDNA encoding human KCTD1 was amplified and cloned into the expression vector pET-30a(+). The recombinant protein was expressed in E. coli BL21(DE3) cells and subsequently purified using affinity chromatography. To confirm that KCTD1 was correctly expressed and folded, the molecular weight and conformation were analyzed using mass spectroscopy, Western blot, and circular dichroism. Optimizing KCTD1 expression and investigating its secondary structure will provide valuable information for future structural and functional studies of KCTD1 and KCTD family proteins.


Assuntos
Proteínas Repressoras , Dicroísmo Circular , Proteínas Correpressoras , Eletroforese em Gel de Poliacrilamida , Escherichia coli/química , Escherichia coli/citologia , Escherichia coli/metabolismo , Humanos , Estrutura Secundária de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Proteínas Repressoras/química , Proteínas Repressoras/isolamento & purificação , Proteínas Repressoras/metabolismo , Espectrofotometria Ultravioleta
14.
Acta Crystallogr Sect E Struct Rep Online ; 68(Pt 4): m430, 2012 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-22589806

RESUMO

In the title complex, [Cu(4)Cl(4)(C(58)H(52)N(2)P(4))](n), four Cu(II) atoms are held together via two doubly bridging and two triply bridging chlorides, forming a stair-like Cu(4)Cl(4) core having crystallographically imposed inversion symmetry, while the benzene-1,4-diamine ligand (with a crystallographic inversion center at the centroid) acts in a tetra-dentate coordination mode, bridging two adjacent Cu(4)Cl(4) cores, resulting in a chain along the a-axis direction. One Cu atom has a distorted tetra-hedral geometry, coordinated by one P atom, one µ(2)-Cl and two µ(3)-Cl atoms, while the second Cu atom adopts a trigonal geometry, coordinated by one P atom, one µ(2)-Cl and one µ(3)-Cl atoms.

15.
Biochem Biophys Res Commun ; 417(1): 182-6, 2012 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-22138399

RESUMO

To identify molecular interaction partners of the cellular prion protein (PrP(C)), we applied a yeast two-hybrid screen on a bovine brain cDNA expression library and identified the potassium channel tetramerization domain containing 1 (KCTD1) as a PrP(C) interacting protein. Deletion mapping showed that PrP(C) specifically binds KCTD1 through the unstructured PrP(51-136) region. We further confirmed the interaction between PrP(C) and KCDT1 protein by co-immunoprecipitation in vivo and by a biosensor assay in vitro. Interestingly, the binding of an insertion mutant PrP(8OR) to KCTD1 is higher than that of wild-type PrP(C), suggesting an important role for an unstructured region harboring octapeptide repeats in the KCTD1-PrP(C) interaction. Our results identify a novel PrP(C)-interacting protein and suggest a new approach to investigating the unidentified physiological cellular function of PrP(C).


Assuntos
Proteínas PrPC/metabolismo , Proteínas Repressoras/metabolismo , Animais , Bovinos , Imunoprecipitação , Oligopeptídeos/metabolismo , Proteínas PrPC/genética , Multimerização Proteica , Sequências Repetitivas de Aminoácidos , Proteínas Repressoras/genética , Técnicas do Sistema de Duplo-Híbrido
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