Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.150
Filtrar
1.
Artigo em Inglês | MEDLINE | ID: mdl-33802188

RESUMO

Environmentally sustainable development is a multidimensional concept that emphasizes the integration of economy, society and environment within a region and the realization of dynamic balance. How to objectively environmentally sustainable development has been a major concern for scholars and policy makers. To address this problem effectively, we first obtain the indicators of environmentally sustainable development based on the pressure-state-response (PSR) framework. Then, we introduce variable weight factors in the traditional analytic hierarchy process (AHP), so that the weights assigned by experts to sustainable development indicators can change with time or space. In this way, we propose a new and improved weight distribution method called variable weigh analytic hierarchy process. Finally, we employ indicators of environmentally sustainable development based on PSR and variable weigh analytic hierarchy process to evaluate the sustainable development of cities in a case country. Our study found that: (1) indicators of environmentally sustainable development should consist of three parts: pressure indicators of environmentally sustainable development, state indicators of environmentally sustainable development, and response indicators of sustainable development; (2) with the variable weigh analytic hierarchy process, our ranking hierarchy process can handle dynamic changes among indicators better than the traditional AHP method and better reflect the true states of indicators.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33822728

RESUMO

The Cox proportional hazards model is one of the most widely used methods for analyzing survival data. Data from multiple data providers are required to improve the generalizability and confidence of the results of Cox analysis; however, such data sharing may result in leakage of sensitive information, leading to financial fraud, social discrimination or unauthorized data abuse. Some privacy-preserving Cox regression protocols have been proposed in past years, but they lack either security or functionality. In this paper, we propose a privacy-preserving Cox regression protocol for multiple data providers and researchers. The proposed protocol allows researchers to train models on horizontally or vertically partitioned datasets while providing privacy protection for both the sensitive data and the trained models. Our protocol utilizes threshold homomorphic encryption to guarantee security. Experimental results demonstrate that with the proposed protocol, Cox regression model training over 9 variables in a dataset of 113,035 samples takes approximately 44 min, and the trained model is almost the same as that obtained with the original nonsecure Cox regression protocol; therefore, our protocol is a potential candidate for practical real-world applications in multicenter medical research.

3.
Med Phys ; 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33825207

RESUMO

PURPOSE: The dual-energy computed tomography (DECT) technique is an emerging imaging tool that can better characterize material features and has the potential to be a noninvasive means of predicting lymph node metastasis. The purpose of this study was to establish a DECT-specified quantitative approach based on a neural network to characterize the sentinel lymph node (SLN). METHODS: With IRB approval, we retrospectively collected a total of 229 patients (100/229 metastasis) with biopsy proven breast cancer in this study. The chest and axillary spectral CT examinations were performed prior to the axillary lymph node (ALN) surgery. A decoupling convolution network with 11 ROIs from sequential keV (40 to 140 keV with 10 keV increment) was proposed to explicitly extract the spectral and spatial features in a DECT to predict the lymph node status. Focal Loss was introduced as the loss function. The metric of the slope of the spectral Hounsfield unit curve measured at the venous phase was used as the baseline approach in comparison to our approach. In additional, a logistic model with radiomic features was also compared to our approach. The area under ROC curves (AUC) was used as the figure of merit to evaluate the classification performance. RESULTS: By introducing spectral convolution and focal loss, AUC on test set could be improved by 0.15 and 0.01 separately. Compared to the slope of the spectral curve with the average AUC of 0.611 and radiomic model with AUC of 0.825, the proposed approach demonstrates a considerably better performance, with test set AUC value of 0.837, by using decoupling spectral and spatial convolution together with focal loss function. CONCLUSIONS: We presented a new decoupling neural network based quantification method for DECT analysis, which might have potential as a noninvasive tool to predict metastasis lymph node status for breast cancer in clinical practice.

4.
Andrologia ; : e14065, 2021 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-33822394

RESUMO

The retrospective cohort study was conducted to evaluate the effectiveness of preimplantation genetic testing for aneuploidy (PGT-A) for severe male factor (SMF) infertility on pregnancy outcomes in comparison with intracytoplasmic sperm injection (ICSI). A total of 206 couples with SMF were included in the study, among which, 102 couples underwent ICSI with next-generation sequencing (NGS)-based PGT-A (the PGT-A group), while 104 underwent ICSI only (the control group). Results showed while no differences were noted in clinical pregnancy rate (CPR) (66.7% versus. 69.9%, p = .64) and ongoing pregnancy rate (OPR) (62.2% versus. 54.7%, p = .29) per transfer between groups, early miscarriage rate (EMR) per transfer was significantly lower (6.7% versus. 21.6%, p = .02) in the PGT-A group. Cumulative OPR per patient remained similar between groups (54.9% versus. 55.8%, p = .90). Results of multivariable logistic regression also demonstrated the use of PGT-A was significantly associated with lower EMR (adjusted OR 0.17, 95%CI 0.05-0.55) in SMF, while it was not related to cumulative OPR. In conclusion, our results showed that NGS-based PGT-A can improve pregnancy outcomes for couples with SMF by significantly decreasing EMR without compromising cumulative OPR, indicating that NGS-based PGT-A could be offered as an appropriate approach for couples with SMF.

5.
Demography ; 58(1): 191-217, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33834242

RESUMO

Deepening democratization in Brazil has coincided with sustained flows of domestic migration, which raises an important question of whether migration deepens or depresses democratic development in migrant-sending regions. Whereas earlier perspectives have viewed migration as a political "brain drain," we contend that out-migration can generate resources that promote democratic processes back home. We investigate the role of migration in two aspects of democratization: electoral participation and competition. The analyses are based on spatial panel data models of mayoral election results across all municipalities between 1996 and 2012. The results show that migration increases electoral participation and competition in migrant-sending localities in Brazil. This study also identifies the sociopolitical context that conditions the impact of migration: the effect is most often present in the context of rural-urban migration and is more pronounced in sending localities with less democratic political structures. Moreover, using spatial network models, we find evidence for the transmission of political remittances from migration destination municipalities to origin municipalities. The present study extends the research on the migration-development nexus to the political arena, thus demonstrating the value of integrating demographic processes into explanations of political change.

6.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 39(2): 143-152, 2021 Apr 01.
Artigo em Chinês | MEDLINE | ID: mdl-33834668

RESUMO

OBJECTIVES: This study aimed to explore the changes in the expression of the characteristic transcription factor retinoid related orphan receptor γt (RORγt) and the cytokine interleukin-17 (IL-17) of T helper cell 17 (Th17) in the pressure side of the periodontal tissue of rats under different orthodontic forces. Their effects on the expression of osteoprotegerin (OPG) and the quantity of osteoclast (OC) were also explored. The role of Th17 cell in alveolar bone remodeling under different forces was preliminarily investigated. METHODS: A total of 108 rats were chosen and randomly divided into three groups. Mesial forces of 0, 50, and 100 g were loaded on the maxillary first molar in the three groups. The rats were executed at 0, 1, 3, 5, 7, and 14 days. The expression of RORγt mRNA was quantified by real-time quantitative polymerase chain reaction. The expression of IL-17 protein was quantified by enzyme linked immunosorbent assay. The expression levels of RORγt and OPG proteins were quantified, and the quantity of OC was counted via immunohistochemistry. RESULTS: The expression levels of RORγt and IL-17 and the quantity of OC increased first and then decreased in the 50 and 100 g groups, and the peak values of the two groups were on days 5 and 7, respectively. The expression levels in the 50 g group basically recovered to normal level on day 14, while that in the 100 g group remained at a high level. The expression levels in the 50 g group were higher than those in the 0 g group and lower than those in the 100 g group. The expression of OPG in the 50 g group decreased first, then increased, and finally decreased. It basically recovered to normal level on day 14. The expression of OPG in the 100 g group decreased first and then increased. It remained at a high level on day 14. The expression in the 50 g group was significantly higher than that in the 0 g group on day 7, while the expression in the 100 g group was significantly higher than that in the 0 g group on day 14. CONCLUSIONS: RORγt, IL-17, and OPG were expressed regularly over time under different orthodontic forces, indicating that Th17 participated in the process of bone resorption on the pressure side of periodontal tissue by secreting IL-17.

7.
IEEE Trans Med Imaging ; PP2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33826513

RESUMO

Synthetic digital mammography (SDM), a 2D image generated from digital breast tomosynthesis (DBT), is used as a potential substitute for full-field digital mammography (FFDM) in clinic to reduce the radiation dose for breast cancer screening. Previous studies exploited projection geometry and fused projection data and DBT volume, with different post-processing techniques applied on re-projection data which may generate different image appearance compared to FFDM. To alleviate this issue, one possible solution to generate an SDM image is using a learning-based method to model the transformation from the DBT volume to the FFDM image using current DBT/FFDM combo images. In this study, we proposed to use a deep convolutional neural network (DCNN) to learn the transformation to generate SDM using current DBT/FFDM combo images. Gradient guided conditional generative adversarial networks (GGGAN) objective function was designed to preserve subtle MCs and the perceptual loss was exploited to improve the performance of the proposed DCNN on perceptual quality. We used various image quality criteria for evaluation, including preserving masses and MCs which are important in mammogram. Experiment results demonstrated progressive performance improvement of network using different objective functions in terms of those image quality criteria. The methodology we exploited in the SDM generation task to analyze and progressively improve image quality by designing objective functions may be helpful to other image generation tasks.

8.
Transl Oncol ; 14(6): 101083, 2021 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-33784583

RESUMO

BACKGROUND: A novel therapy based on programmed death 1 (PD-1) inhibitors has been proved to be effective in advanced esophageal cancer. This article is a meta-analysis that aims to compare the efficacy and safety of anti-PD-1 therapy with chemotherapy in esophageal cancer. PATIENTS AND METHODS: Data were collected from eligible studies searched from PubMed, Web of Science, Cochrane Library, and Embase. Pooled hazard ratio (HR) for overall survival (OS), progression-free survival (PFS), and objective response rate (ORR) was estimated to assess the efficacy of PD-1 inhibitors versus chemotherapy. The subgroup analysis was also performed to evaluate the OS benefits. The OR for the occurrence of treatment-related adverse effects was calculated to assess the safety of anti-PD-1 therapy. RESULTS: A total of 4 studies were analyzed. Compared with patients with chemotherapy, patients with anti-PD-1 therapy had a significant improvement in OS (HR = 0.79, 95% CI: 0.71-0.88, and P<0.001), but no significant relationship was observed in PFS (HR = 0.96, 95% CI: 0.76-1.20, and P = 0.69) and ORR (OR = 1.92, 95% CI: 0.98-3.72, and P = 0.06). A similar result was observed in esophageal squamous cell carcinoma. The significant predictor for treatment benefit alone was histology (P = 0.009). The incidence of grade 3 - 5 treatment-related adverse effects in anti-PD-1 therapy was distinctly lower than that in chemotherapy, but there is no statistical difference in all treatment-related adverse effects. CONCLUSION: Anti-PD-1 therapy significantly prolonged the OS, simultaneously lowered grade 3 - 5 treatment-related adverse effects versus chemotherapy.

9.
Biomed Res Int ; 2021: 5450397, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33763470

RESUMO

Objective: We aimed to establish a method to determine whether microRNA-193b (miR-193b) levels in ABCA1-labeled serum exosomes might serve as a marker for the diagnosis of Alzheimer's disease. Methods: We used immunocapture methods to determine the levels of ABCA1-labeled exosomal miR-193b in cultures of white blood cells (WBCs), red blood cells (RBCs), mouse hippocampal neuron HT-22 cells, and primary mouse neuronal cells. ABCA1-labeled exosomal miR-193b levels were also evaluated in the cerebrospinal fluid (CSF) and serum of APP/PS1 double-transgenic mice, as well as control subjects (n = 60) and study participants with subjective cognitive decline (SCD, n = 89), stage and mild cognitive impairment (MCI, n = 92), and dementia of the Alzheimer type (DAT, n = 92). Results: ABCA1 levels of exosomes harvested from the medium of HT-22 cells and neurons were significantly higher than those of RBCs and WBCs (P < 0.05). Exosomal ABCA1 from the CSF of APP/PS1 mice were transmitted to the serum of wild-type mice after injection, and high miR-193b levels were observed in both the serum and CSF after injection. The ABCA1-labeled exosomal miR-193b levels were higher in the CSF of MCI and DAT patients compared with the CSF of the control group (P < 0.05). The ABCA1-labeled exosomal miR-193b were also slightly higher (P > 0.05) in the serum of SCD patients and significantly higher in the serum of MCI and DAT patients compared with the serum of the control group (P < 0.05). Conclusion: This study provides a method to capture specific exosomes. Detection of serum exosomes labeled with ABCA1 may facilitate the early diagnosis of AD.

11.
J Chromatogr Sci ; 2021 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-33778859

RESUMO

The stationary phase based on sol-gel ground silica monolith particles has been produced by one-pot polymerization method incorporation of styrene and ethylene dimethacrylate. First, the ground silica monolith particles were prepared by a sol-gel process followed by sedimentation. The particles were then subjected to modify with styrene ligand via one-pot polymerization, whereas ethylene dimethacrylate was used as the cross-linker. The glass lined stainless steel columns (1 mm internal diameter, 150 mm length) were packed with the above phase for estimation of the chromatographic performance in high-performance liquid chromatography. An average number of theoretical plates of as high as 39,300 plates/column was obtained under the optimized elution condition. The column-to-column reproducibility was proved satisfactory in separation efficiency and retention factor. The experimental results indicate that sol-gel ground silica particles prepared by an aid of one-pot modification can provide a better way for preparation of highly efficient stationary phase.

12.
IEEE Trans Cybern ; PP2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33750731

RESUMO

In convolutional neural networks (CNNs), generating noise for the intermediate feature is a hot research topic in improving generalization. The existing methods usually regularize the CNNs by producing multiplicative noise (regularization weights), called multiplicative regularization (Multi-Reg). However, Multi-Reg methods usually focus on improving generalization but fail to jointly consider optimization, leading to unstable learning with slow convergence. Moreover, Multi-Reg methods are not flexible enough since the regularization weights are generated from a definite manual-design distribution. Besides, most popular methods are not universal enough, because these methods are only designed for the residual networks. In this article, we, for the first time, experimentally and theoretically explore the nature of generating noise in the intermediate features for popular CNNs. We demonstrate that injecting noise in the feature space can be transformed to generating noise in the input space, and these methods regularize the networks in a Mini-batch in Mini-batch (MiM) sampling manner. Based on these observations, this article further discovers that generating multiplicative noise can easily degenerate the optimization due to its high dependence on the intermediate feature. Based on these studies, we propose a novel additional regularization (Addi-Reg) method, which can adaptively produce additional noise with low dependence on intermediate feature in CNNs by employing a series of mechanisms. Particularly, these well-designed mechanisms can stabilize the learning process in training, and our Addi-Reg method can pertinently learn the noise distributions for every layer in CNNs. Extensive experiments demonstrate that the proposed Addi-Reg method is more flexible and universal, and meanwhile achieves better generalization performance with faster convergence against the state-of-the-art Multi-Reg methods.

13.
ACS Appl Mater Interfaces ; 13(12): 14327-14333, 2021 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-33739087

RESUMO

Herein, we fabricated an Ag/Ag2Se composite film on a flexible nylon membrane with a high power factor and excellent flexibility. First, Ag nanoparticles and multiscale Ag2Se nanostructure composite powders were prepared by wet chemical synthesis using Se nanowires, silver nitrate, and l-ascorbic acid as raw materials, followed by vacuum-assisted filtration of the composite powders on a porous nylon membrane and then hot pressing. The optimized composite film shows a very high power factor of 1860.6 µW m-1 K-2 (with a corresponding electrical conductivity of 3958 S cm-1) at room temperature. The composite film retains 93.3% of the original electrical conductivity after 1000 bending cycles around a rod with a diameter of 8 mm. At a temperature difference of 27 K, an 8-leg thermoelectric prototype device assembled with the optimized composite film generates a maximum power of 7.14 µW with a corresponding power density of 8.74 W m-2. This work provides a new strategy to synthesize flexible thermoelectric films with both a high power factor and high electrical conductivity.

14.
J Sep Sci ; 2021 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-33683009

RESUMO

A novel chiral open tubular capillary column was fabricated with sulfobutylether-ß-cyclodextrin and glycidyl methacrylate for enantioseparation in capillary electrochromatography. First, the pretreated silica-fused capillary was treated with 3-trimethoxysilyl propyl methacrylate to attach double bond ligand onto the surface. A copolymer layer was formed on the surface of capillary using glycidyl methacrylate and ethylene dimethacrylate by in situ one-pot polymerization. Sulfobutylether-ß-cyclodextrin was encapsulated inside the copolymerized layer. The morphology of the developed column was characterized by field emission scanning electron microscopy. The effect of organic percentage and pH value of the mobile phase on electroosmotic flow and resolution was also investigated. The performance of the fabricated column was validated by separation of amlodipine besilate, 2,3-diphenylpropionic acid, tropic acid, and pantoprazole enantiomers with good resolutions of 3.67, 4.82, 3.34, and 2.61, respectively. The repeatabilities of column-to-column and day-to-day through relative standard deviation were found better than 4%, exhibiting satisfactory repeatability of the developed column. The results reveal that open tubular capillary columns modified with ß-cyclodextrin show a great prospect for enantioseparation of chiral drugs in capillary electrochromatography.

15.
Sci Adv ; 7(11)2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33712456

RESUMO

Traumatic brain injury (TBI) leads to high mortality rate. We aimed to identify the key cytokines favoring TBI repair and found that patients with TBI with a better outcome robustly increased concentrations of macrophage colony-stimulating factor, interleukin-6, and transforming growth factor-ß (termed M6T) in cerebrospinal fluid or plasma. Using TBI mice, we identified that M2-like macrophage, microglia, and endothelial cell were major sources to produce M6T. Together with the in vivo tracking of mCherry+ macrophages in zebrafish models, we confirmed that M6T treatment accelerated blood-borne macrophage infiltration and polarization toward a subset of tissue repair macrophages that expressed similar genes as microglia for neuroprotection, angiogenesis and cell migration. M6T therapy in TBI mice and zebrafish improved neurological function while blocking M6T-exacerbated brain injury. Considering low concentrations of M6T in some patients with poor prognostic, M6T treatment might repair TBI via generating a previously unidentified subset of tissue repair macrophages.

16.
PLoS One ; 16(3): e0247578, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33711032

RESUMO

To select the optimum fertilizer application under specific irrigation levels and to provide a reliable fertigation system for tomato plants, an experiment was conducted by using a microporous membrane for water-fertilizer integration under non-pressure gravity. A compound fertilizer (N:P2O5:K2O, 18:7:20) was adopted for topdressing at four levels, 1290 kg/ha, 1140 kg/ha, 990 kg/ha, and 840 kg/ha, and the locally recommended level of 1875 kg/ha was used as the control to explore the effects of different fertilizer application rates on growth, nutrient distribution, quality, yield, and partial factor of productivity (PFP) in tomato. The new regime of microporous membrane water-fertilizer integration under non-pressure gravity irrigation reduced the fertilizer application rate while promoting plant growth in the early and intermediate stages. Except for the 990 kg/ha fertilizer treatment, yields per plant and per plot for each fertilizer application rate were higher than or equal to those of the control. The new regime could effectively improve PFP and reduce soil nutrient enrichment. Fertilizer at 840 kg/ha showed the optimum results by increasing PFP by 75.72% as compared to control. In conclusion, the fertilizer rate at 840 kg/ha has not only maintained the productivity of soil but also tomato growth and quality of fruit which makes the non-pressure gravity irrigation a potential and cost-effective way for fertilizer application.

17.
J Orthop Surg Res ; 16(1): 197, 2021 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-33731159

RESUMO

BACKGROUND: The surgical approaches remain controversial for the treatment of middle and distal-third humeral shaft (MDTHS) fractures. This study compared clinical effects of the anterolateral approach with two incisions (AATI) and the posterior median approach (PMA) in the treatment of MDTHS fractures. METHODS: A retrospective analysis was carried out. One hundred sixty-six patients with MDTHS fractures were selected from January 2015 to January 2017 in Xi'an Hong Hui Hospital. According to surgical approaches, patients were divided into AATI (86 cases) and PMA group (80 cases). All patients were treated with open reduction and plate fixation. Operation indexes were compared, including incision length, operation time, and bleeding. Bryan-Morrey score was used to evaluate elbow joint function. Complication incidence was compared, such as incision infection, iatrogenic radial nerve injury, and nonunion. RESULTS: The AATI group showed smaller incision length, less bleeding, lower iatrogenic radial nerve injury rate, and better elbow function than that of PMA group (P<0.05). CONCLUSIONS: The middle and distal-third humeral shaft fractures can be successfully cured by both approaches. Compared with the posterior median approach, it has better clinical effects of the anterolateral approach with two incisions, which is worthy of clinical application and promotion.

18.
Clin Lab ; 67(3)2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33739049

RESUMO

BACKGROUND: Severe pneumonia (SP) is a clinically critical acute disease which has a higher mortality rate among infectious diseases. In this report, a rare case of severe pneumonia with severely high lactic acid (up to 24 mmol/L) and relatively normal pH was analyzed. METHODS: The case was discussed from different angles including acid-base balance disorder, the use of extractor-poreal membrane oxygenation (ECMO), dialysis treatment, circulatory disturbance, and inspection methodology. RESULTS: Hypoxia and dissolution of muscles caused by circulatory disorders may be the cause of the abnormal increase of lactate in this case; while the relatively normal pH may be caused by the dialysis treatment. CONCLUSIONS: Such a high blood gas lactic acid value is extremely rare, and this increase is not due to the limitations of the test method. High lactic acid may not result in the significant decrease of pH when the patient receives continuous systemic treatment.

19.
BMC Anesthesiol ; 21(1): 69, 2021 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-33685395

RESUMO

BACKGROUND: The peak inspiratory pressure (PIP) is crucial in mechanical ventilation with supraglottic airway device (SAD). Pressure-controlled ventilation volume-guaranteed (PCV-VG), delivering a preset tidal volume with the lowest required airway pressure, is being increasingly used during general anesthesia. In this study, we compared respiratory mechanics and circulatory parameters between volume-controlled ventilation (VCV) and PCV-VG in elderly patients undergoing laparoscopic surgery using the laryngeal mask airway supreme (LMA). METHODS: Eighty participants scheduled for laparoscopic surgery were enrolled in this prospective, randomized clinical trial. The participants were randomly assigned to receive VCV or PCV-VG. PIP, dynamic compliance (Cdyn) and mean inspiratory pressure (Pmean) were recorded at 5 min after induction of anesthesia (T1), 5 min after pneumoperitoneum(T2), 30 and 60 min after pneumoperitoneum (T3 and T4). Data including other respiratory variables, hemodynamic variables, and arterial blood gases were also collected. The difference in PIP between VCV and PCV-VG was assessed as the primary outcome. RESULTS: PIP was significantly lower at T2, T3, and T4 in both groups compared with T1 (all P <  0.0001), and it was significantly lower in the PCV-VG group than the VCV group at T2, T3, and T4 (all P <  0.001). Cydn was decreased at T2, T3, and T4 in two groups compared with T1 (all P <  0.0001), but it was higher in PCV-VG group than in VCV group at T2, T3, and T4 (all P <  0.0001). There were on statistically significant differences were found between the groups for other respiratory and hemodynamic variables. CONCLUSION: In elderly patients who underwent laparoscopic surgery using an LMA, PCV-VG was superior to VCV in its ability to provide ventilation with lower peak inspiratory pressure and greater dynamic compliance.

20.
Oxid Med Cell Longev ; 2021: 6691226, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33680286

RESUMO

Diabetic kidney disease (DKD) is the major cause of end-stage renal disease (ESRD). In the past few decades, there has been a large amount of evidence to highlight the pivotal role of oxidative stress in the development and progression of DKD. However, the detailed molecular mechanisms are not fully elucidated. A new sight has been established that the mitochondrial acetyltransferase GCN5L1 participates in cellular redox homeostasis maintenance in DKD. Firstly, we found that the expression of GCN5L1 is significantly elevated both in human and mouse kidney tissues with DKD and in hyperglycemic renal tubular epithelial cells (TECs), while deletion of GCN5L1 could effectively ameliorate oxidative stress-induced renal injury in DKD. Furthermore, deletion of GCN5L1 could reduce MnSOD acetylation on lysine 68 and activate its activity, thereby scavenging excessive ROS and relieving oxidative stress-induced renal inflammation and fibrosis. In general, GCN5L1-mediated acetylation of MnSOD exacerbated oxidative stress-induced renal injury, suggesting that GCN5L1 might be a potential intervention target in DKD.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...