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1.
Braz Dent J ; 31(1): 57-62, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32159707

RESUMO

The objective of this study was to investigate the effects of low-level laser therapy (LLLT) and cigarette smoke on alveolar socket osteoclastogenesis signaling after tooth extraction, in rats. Sixty male Wistar rats were randomly assigned to four groups with 15 animals each: Control Group (with right maxillary molar extraction - ME), Experimental I (with ME and LLLT), Experimental II (with ME and cigarette smoke) and Experimental III group (with ME, LLLT and cigarette smoke). Euthanasia was performed at 3, 7 and 14 days postoperative. qRT-PCR was used to evaluate expression of Tnfrsf11a (RANK), Tnfsf11 (Rankl) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Bonferroni test (α=0.05). There was an upregulation of RANK, RANKL and OPG genes over all the time of healing in Exp I group compared to control group. Exp II group showed a decreased expression of all genes over time, whereas Exp III genes expression were higher than Exp II values but lower than Control and Exp I values over time. The results of this study concluded that the LLLT had a positive effect, whereas cigarette smoke had a negative effect on RANK, RANKL and OPG gene expression in bone remodeling process.


Assuntos
Fumar Cigarros , Terapia com Luz de Baixa Intensidade , Animais , Masculino , Ratos , Ratos Wistar , Extração Dentária , Cicatrização
2.
Braz. dent. j ; 31(1): 57-62, Jan.-Feb. 2020. graf
Artigo em Inglês | LILACS-Express | ID: biblio-1089270

RESUMO

Abstract The objective of this study was to investigate the effects of low-level laser therapy (LLLT) and cigarette smoke on alveolar socket osteoclastogenesis signaling after tooth extraction, in rats. Sixty male Wistar rats were randomly assigned to four groups with 15 animals each: Control Group (with right maxillary molar extraction - ME), Experimental I (with ME and LLLT), Experimental II (with ME and cigarette smoke) and Experimental III group (with ME, LLLT and cigarette smoke). Euthanasia was performed at 3, 7 and 14 days postoperative. qRT-PCR was used to evaluate expression of Tnfrsf11a (RANK), Tnfsf11 (Rankl) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Bonferroni test (α=0.05). There was an upregulation of RANK, RANKL and OPG genes over all the time of healing in Exp I group compared to control group. Exp II group showed a decreased expression of all genes over time, whereas Exp III genes expression were higher than Exp II values but lower than Control and Exp I values over time. The results of this study concluded that the LLLT had a positive effect, whereas cigarette smoke had a negative effect on RANK, RANKL and OPG gene expression in bone remodeling process.


Resumo O objetivo deste estudo foi investigar os efeitos da terapia a laser de baixo nível (LLLT) e a fumaça de cigarro na sinalização da osteoclastogênese do alvéolo após extração dentária, em ratos. Sessenta ratos Wistar machos foram distribuídos aleatoriamente em quatro grupos com 15 animais cada: Grupo de controle (com extração do molar superior direito (EM), Experimental I (EM e LLLT), Experimental II (EM e fumaça de cigarro) e Grupo Experimental III (EM, LLLT e fumaça de cigarro). A eutanásia foi realizada aos 3, 7 e 14 dias após a extração. O qRT-PCR foi utilizado para avaliar a expressão de Tnfrsf11a (RANK), Tnfsf11 (RANKL) e Tnfrsf11b (OPG). Os dados foram submetidos à análise estatística usando two-way ANOVA seguido do teste de Bonferroni (α=0,05). Houve um aumento na expressão dos genes RANK, RANKL e OPG ao longo do tempo de cicatrização no grupo Exp I em comparação com o grupo controle. O grupo Exp II mostrou uma expressão diminuída de todos os genes ao longo do tempo, enquanto a expressão dos genes do grupo Exp III foi superior aos valores observados em Exp II, mas inferiores aos valores do grupo Controle e Exp I ao longo do tempo. Os resultados deste estudo concluíram que o LLLT tem um efeito positivo, enquanto a fumaça de cigarro possui efeito prejudicial na expressão gênica de RANK, RANKL e OPG no processo de remodelação óssea.

3.
Rio de Janeiro; s.n; 2018. 88 p. tab, ilus.
Tese em Português | BBO - Odontologia | ID: biblio-1016009

RESUMO

O objetivo deste estudo foi avaliar os efeitos da expansão rápida da maxila (ERM) na polpa dentária de dentes de ancoragem de ratos jovens, através das análises histomorfológica, histomorfométrica e de expressão gênica. Oitenta (n=80) ratos Wistar machos foram distribuídos aleatoriamente em 2 grupos: Grupo Controle (GC, n=40), em que os animais não tiveram ERM e foram sacrificados nos períodos de 3, 7, 14 e 21 dias após o início do experimento; e Grupo Experimental (GE, n=40), cujos animais foram submetidos à ERM e sacrificados nos mesmos períodos do Grupo Controle. As polpas dentárias dos incisivos superiores de 20 animais (n=20) de cada grupo, GC e GE, foram extraídas para a análise da expressão gênica do RNAm, pela técnica de Reação em Cadeia da Polimerase em Tempo Real (RT-PCR), para os genes Fator de Crescimento Endotelial Vascular (Vegf), Sialofosfoproteína da Dentina (Dspp) e Ciclooxigenase-2 (Cox-2), e de 20 animais (n=20) para as análises histomorfológica e histomorfométrica do tecido pulpar. Todos os grupos que sofreram ERM apresentaram sinais da ocorrência de inflamação e aumento da densidade de vasos sanguíneos, com alterações transitórias na camada de odontoblastos. Houve aumento significativo da expressão gênica de Vegf em todos os períodos experimentais, sendo para Cox-2 apenas nos períodos de 3 e 7 dias, e para Dspp no 7º e 14º dias. Concluise que a ERM induziu a remodelação do tecido pulpar, com modulação transitória das expressões gênicas analisadas e da vascularização. (AU)


The objective of this study was to evaluate the effects of rapid maxillary expansion (RME) on the dental pulp of anchoring teeth of young rats through histolomorphological, histomorphometric and gene expression analyzes. Eighty (n= 80) male Wistar rats were randomly assigned to 2 groups: Control Group (CG, n=40), in which the animals had no ERM and were sacrificed at 3, 7, 14 and 21 days after initiation of the experiment; and Experimental Group (GE, n=40), whose animals were submitted to RME and sacrificed in the same periods of the GC. The dental pulps of the upper incisors of twenty animals (n=20) from each group, GC and GE, were extracted for analysis of the mRNA gene expression by the Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique for the Vascular Endothelial Growth Factor (Vegf), Dentin Sialophosphoprotein (Dspp) and Cyclooxygenase-2 (Cox-2) genes, and twenty animals (n=20) for the histolomorphological and histomorphometric analyzes of the pulp tissue. All groups that suffered RME showed signs of inflammation occurrence and increased blood vessel density, with transient changes in the odontoblast layer. There was a significant increase in Vegf gene expression in all experimental periods, being for Cox-2 only in periods of 3 and 7 days, and for Dspp in the 7th and 14th days. It was concluded that ERM induced remodeling of pulp tissue, with transient modulation of the analyzed gene expression and vascularization. (AU)


Assuntos
Animais , Ratos , Expressão Gênica , Técnica de Expansão Palatina/efeitos adversos , Polpa Dentária/anatomia & histologia , Ratos Wistar , Procedimentos de Ancoragem Ortodôntica/efeitos adversos
4.
Biomed Res Int ; 2014: 563463, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25028660

RESUMO

Although disorders of the stomatognathic system are common, the mechanisms involved are unknown. Our objective was to study the changes in the masseter muscles after unilateral exodontia. Molar extraction was performed on Wistar rats (left side), and the animals were sacrificed after either 14 or 26 days. The masseter muscle was processed for histological analysis, conventional and in situ zymography, and immunohistochemistry. The morphological analysis showed unique and specific characteristics for the experimental group. By conventional zymography no significant values of 72 kDa MMP-2 (P < 0.05) were found in both of the sides of masseter muscle after 14 and 26 days of unilateral extraction. The in situ zymography showed gelatinolytic activity on all deep masseter muscles, with significant increase on the contralateral side after 14 and 26 days (P < 0.05). The immunohistochemistry demonstrated greater expression of MMP-2 than MMP-9 and MMP-14 in all masseter muscles and there were few differences in the staining of 4 TIMPs. This knowledge about morphology and molecular masticatory muscle remodeling following environmental interventions can be used to develop clinically successful treatments.


Assuntos
Má Oclusão/metabolismo , Músculo Masseter/metabolismo , Metaloproteinases da Matriz/metabolismo , Proteínas Musculares/metabolismo , Inibidores Teciduais de Metaloproteinases/metabolismo , Animais , Modelos Animais de Doenças , Masculino , Má Oclusão/patologia , Músculo Masseter/patologia , Coelhos , Ratos Wistar
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