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1.
Mater Sci Eng C Mater Biol Appl ; 128: 112310, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34474861

RESUMO

Intervertebral disc (IVD) degeneration is a clinically disease that seriously endangers people's health. Tissue engineering provides a promising method to repair and regenerate the damaged IVD physiological function. Successfully tissue-engineered IVD scaffold should mimic the native IVD histological and macro structures. Here, 3D printing and electrospinning were combined to construct an artificial IVD composite scaffold. Poly lactide (PLA) was used to print the IVD frame structure, the oriented porous poly(l-lactide)/octa-armed polyhedral oligomeric silsesquioxanes (PLLA/POSS-(PLLA)8) fiber bundles simulated the annulus fibrosus (AF), and the gellan gum/poly (ethylene glycol) diacrylate (GG/PEGDA) double network hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) simulated the nucleus pulposus (NP) structure. Morphological and mechanical tests showed that the structure and mechanical properties of the IVD scaffold were similar to that of the natural IVD. The compression modulus of the scaffold is about 10 MPa, which is comparable to natural IVD and provides good mechanical support for tissue repair and regeneration. At the same time, the porosity and mechanical properties of the scaffold can be regulated through the 3D model design. In the AF structure, the fiber bundles are oriented concentrically with each subsequent layer oriented 60° to the spinal column, and can withstand the tension generated during the deformation of the NP. In the NP structure, BMSCs were evenly distributed in the hydrogel and could maintain high cell viability. Animal experiment results demonstrated that the biomimetic artificial IVD scaffold could maintain the disc space and produce the new extracellular matrix. This engineered biomimetic IVD scaffold is a promising biomaterial for individualized IVD repair and regeneration.


Assuntos
Degeneração do Disco Intervertebral , Disco Intervertebral , Animais , Biomimética , Humanos , Degeneração do Disco Intervertebral/terapia , Impressão Tridimensional , Engenharia Tecidual , Tecidos Suporte
2.
Int J Biol Macromol ; 185: 77-86, 2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34139244

RESUMO

Nanostructure provides a closer structural support approximation to native bone architecture for cells and further regulates cell's behavior, resulting in the formation of functional tissues. In this work, three engineering collagen templates with oriented fiber architectures were fabricated via electrospinning (Es), plastic compression and tensile (PCT), and dynamic shear stress (SS) methods. Under the observation of POM, SEM, AFM and TEM, the PCT-template and SS-template are packed with well-oriented nanofibers with the native collagen architecture of 67 nm D-periodicity, and the mechanical properties conferred to the templates are better than that of the Es-template. When mentioning the cell's behavior, MC3T3-E1 adhered to grow along the alignment of collagen fiber orientation when cultured on the PCT-template and SS-template. The SS-template with nano- and micro-ordered architecture guided cells to stretch their plasma along with the orientation of collagen fiber, produce more aligned Type I collagen fibers and promote significantly higher osteogenic differentiation of MC3T3-E1 than the PCT-template and Es-template. Overall, it is strongly argued the feasibility of hierarchical collagen fiber architectures for bone tissue regeneration.


Assuntos
Colágeno/química , Osteoblastos/citologia , Engenharia Tecidual/métodos , Células 3T3 , Animais , Diferenciação Celular , Proliferação de Células , Camundongos , Nanofibras/química , Osteogênese , Ratos , Estresse Mecânico , Tecidos Suporte
3.
ACS Appl Mater Interfaces ; 13(9): 11356-11368, 2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33634690

RESUMO

To solve the damage to the environment and human body caused by organic solvent adhesives in the utilization process, chitin nanocrystal (ChNC) suspension is explored as a strong anisotropic adhesive, which is an eco-friendly and water-based adhesive with high adhesive strength. ChNCs extracted from crab shells are rod-like nanoparticles with high aspect ratios, which are mainly employed as reinforcing polymer nanocomposites and biomedicine nanomaterials. ChNC suspension sandwiched between substrates forms a long-range ordered superstructure by a self-assembly process. ChNC nanoglue exhibits high anisotropy adhesion strength, i.e., an in-plane shear strength (5.26 MPa) and an out-of-plane shear strength (0.46 MPa) for glass substrates. Moreover, the ChNC nanoglue is suitable to many substrates, such as glass, plastic, wood, metal, paper, etc. The ChNC nanoglue shows high biocompatibility toward the fibroblast cell and rat skin, proving their excellent biosafety. As an eco-friendly and high-performance adhesive, ChNC nanoglue shows promising applications in daily life and industrial fields.

4.
Nanoscale ; 12(35): 18225-18239, 2020 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-32856644

RESUMO

Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair. Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix. Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks. Then, the CP porous composite scaffolds were further fabricated via the direct ink writing method. The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 µm and a porosity of 80 ± 5%. Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs. Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0-20 wt%. However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds. Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties. These robust CP composite scaffolds offer a new route for bone tissue engineering application.


Assuntos
Engenharia Tecidual , Tecidos Suporte , Animais , Osso e Ossos , Quitina , Tinta , Poliésteres , Porosidade , Vibrissas , Redação
5.
Int J Biol Macromol ; 157: 24-35, 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32335108

RESUMO

The liquid crystalline and rheological properties of chitin whiskers (CHWs) are significant for their application in fabrication of highly ordered composite materials and optical components. The aim of this work was to elucidate the influence of chemical structure and chargeability (zeta potential, electropositivity, electronegativity or zwitterionic character) on the liquid crystalline and rheological properties of CHWs. Firstly, CHWs with different chemical structure, including positively charged whiskers (CHWs and CHWs-D/60 min) and negatively charged whiskers (mCHWs), were designed via acid hydrolysis, deacetylation, and maleation, respectively. Subsequently, the chargeability of the above whiskers was further regulated by protonation or deprotonation. The whisker aqueous suspensions with high zeta potential behaved as nematic liquid crystals or chiral nematic liquid crystals, whereas those with low zeta potential had no liquid crystal characteristics. The viscosity, G', and G" values of the CHWs and CHWs-D/60 min aqueous suspensions treated with protonation were lower than those of the corresponding whiskers treated with deprotonation. However, the mCHWs exhibited different changes in their rheological properties under protonation or deprotonation due to the electronegativity and zwitterionic characteristics. In addition, the effects of ionic strength and pH on the liquid crystalline and rheological properties of CHWs, CHWs-D/60 min, and mCHWs aqueous suspensions varied since the chemical structure and chargeability of whiskers differ.


Assuntos
Quitina/química , Cristais Líquidos/química , Reologia , Biomimética , Concentração de Íons de Hidrogênio , Água/química
6.
Mater Sci Eng C Mater Biol Appl ; 107: 110333, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31761202

RESUMO

The mechanical environment of extracellular matrix (ECM) plays an important role in adjusting the behaviors of cells. Natural ECM are highly viscoelastic materials with stress-relaxion behavior. Hydrogel is considered as a promising and attractive material for cell carrier, but they are typically elastic serving as synthetic ECM. Double-network (DN) hydrogel has an interpenetrating network of special structure combining the advantages of both rigid and ductile components, due to which the mechanical properties of the system can be very different from that of the single-network ones, and some special biological properties can be obtained. In this study, GG/PEGDA DN hydrogel was prepared by combining gellan gum (GG) with polyethylene glycol diacrylate (PEGDA), and then the influence of the two individual networks on the viscoelasticity of the system were investigated. Furthermore, the effects of viscoelasticity of GG/PEGDA DN hydrogel on the biological behavior of bone mesenchymal stem cells (BMSCs) were explored in vitro and in vivo. The results indicate that the spreading of BMSCs was closely related to the relaxation behavior of the hydrogels. GG/PEGDA DN hydrogel shows excellent mechanical and relaxation properties which provide a favorable physical environment for cell proliferation and spreading, and induce chondrogenic differentiation. Our study demonstrates that this DN hydrogel has bright prospects in the fields of cell carrier and cartilage tissue engineering.


Assuntos
Condrogênese/fisiologia , Hidrogéis/química , Células-Tronco Mesenquimais/citologia , Implantes Absorvíveis , Animais , Fenômenos Biomecânicos , Osso e Ossos/citologia , Cartilagem/fisiologia , Diferenciação Celular , Células Cultivadas , Colágeno Tipo II/metabolismo , Masculino , Camundongos , Polissacarídeos Bacterianos/química , Regeneração , Reologia , Fator de Crescimento Transformador beta3/administração & dosagem , Fator de Crescimento Transformador beta3/metabolismo , Substâncias Viscoelásticas/química
7.
Carbohydr Polym ; 229: 115517, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31826521

RESUMO

In this study, a poly(d,l-lactide) (PDLLA) membrane was prepared by the solution casting method, then the surface of the membrane was modified by polydopamine (PDOPA) as a substrate, followed by adsorption of different chitosan derivative sulfonated chitosan (SCS) or/and phosphorylated chitosan (PCS) to obtain different functionalized membranes, and two kinds of chitosan derivatives characterized by FTIR, elemental analysis and zeta potential. And different membranes were evaluated through surface potential, hydrophilicity, surface morphology and chemical compositions. In vitro, the cell culture results showed that the membrane functionalized by chitosan derivative could promote the proliferation of MC3T3-E1s and enhance the osteogenic differentiation by up-regulating the expression level of osteogenic genes compared to the PDLLA and P1/PDOPA membranes. Especially, when the outermost layer was SCS, the effect of promoting cell proliferation was better than that of PCS. However, for osteogenic differentiation, PCS had better quantitative experimental results than SCS. Therefore, SCS has superiority in promoting proliferation than PCS, but PCS is opposite in promoting osteogenic differentiation for MC3T3-E1s. The results suggested that PCS and SCS have the potential value to be used as a functional modified materials applied in bone tissue engineering.

9.
Biomed Mater ; 14(5): 055005, 2019 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-31271155

RESUMO

A method to mediate biomineralization of electrospinning poly(L-lactide) (PLLA) fibrous membrane assisted by polydopamine (PDA) coating was developed to obtain enhanced osteoconductive activity. The biomineralization mechanism, surface composition, morphology and hydrophilicity of the original and modified PLLA fibrous membranes were characterized. Results revealed that the PDA coating effectively accelerated the formation of hydroxyapatite (HA) on PLLA fibrous membrane and resulted a great increase in hydrophilicity. Moreover, the tensile property of PLLA fibrous membrane was enhanced by the PDA coating while almost kept unchanged by further immobilized with HA. Cells culture results indicated that the successive introduction of PDA and HA contributed to an obvious improvement in the adhesion and proliferation, as well as up-regulated alkaline phosphatase (ALP) activity and promoted osteogenic-related genes and proteins expression of MC3T3-E1 cells. Overall, the as-prepared PLLA-PDA-HA fibrous membrane can be expected as a favorable scaffold for bone tissue repair.


Assuntos
Biomineralização , Regeneração Óssea , Substitutos Ósseos/química , Durapatita/química , Indóis/química , Poliésteres/química , Polímeros/química , Tecidos Suporte , Células 3T3 , Fosfatase Alcalina/metabolismo , Animais , Adesão Celular , Proliferação de Células , Perfilação da Expressão Gênica , Teste de Materiais , Camundongos , Osteogênese , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de Superfície , Resistência à Tração , Termogravimetria , Difração de Raios X
10.
Mater Sci Eng C Mater Biol Appl ; 103: 109851, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31349474

RESUMO

Favorable cytocompatibility and osteogenesis potential are critical for the development of a bone repair material. In this study, two types of surface-modified whiskers, grafted magnesia and chitin (g-MgO and g-CHN) whiskers, were synthesized and introduced into a poly(l-lactide) (PLLA) matrix singly or together to prepare PLLA/g-MgO/g-CHN composite films and bone nails via injection molding. On the account of the synergetic contribution of g-MgO and g-CHN whiskers, the enhanced cell adhesion, spreading, proliferation of mouse embryo osteoblast precursor (MC3T3-E1) cells, as well as the alteration of cell-cycle and inhibition of cell apoptosis, were observed on PLLA/g-MgO/g-CHN film as compared to pure PLLA, PLLA/g-MgO and PLLA/g-CHN films. More importantly, the highest level of the secretion of ALP and the formation of calcium deposition, accompanied with expression of osteogenesis genes (ALP, Runx-2, COL I, OCN) in vitro were obtained for the PLLA/g-MgO/g-CHN film among all of the material groups. Additionally, the PLLA and PLLA composite bone nails were implanted in rabbits' femurs and new bone formation was detected on PLLA/g-MgO/g-CHN group after 16 weeks of implantation by 3D reconstruction of micro-CT and histological analyses. Besides, the bending strength of defected bone repaired by PLLA/g-MgO/g-CHN bone nail was high to 48 MPa, which was far stronger than other bone nail groups. Overall, this study demonstrated the addition of g-MgO and g-CHN whiskers together in PLLA matrix played a synergistic promoting role in cell affinity and osteogenic differentiation, and the developed PLLA/g-MgO/g-CHN composites hold great potential in fields of bone repair.


Assuntos
Pinos Ortopédicos , Quitina/química , Fêmur , Óxido de Magnésio/química , Membranas Artificiais , Osteogênese , Poliésteres/química , Animais , Linhagem Celular , Fêmur/diagnóstico por imagem , Fêmur/lesões , Fêmur/metabolismo , Camundongos , Osteoblastos/metabolismo , Osteoblastos/patologia , Coelhos , Microtomografia por Raio-X
11.
Colloids Surf B Biointerfaces ; 179: 299-308, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30981065

RESUMO

Designing reasonably priced bioactive organic-inorganic hybrid materials featuring osteogenic activity has received a significant interest in the bone tissue engineering field. In this study, eggshell membrane (ESM), which consists mostly of proteins exhibiting fibrous network structure, was utilised innovatively as a template to prepare eggshell membrane/hydroxyapatite (ESM-HA) composites using a versatile biomimetic mineralisation technique. In addition, the surface morphology and composition, hydrophilicity, crystallinity, and thermal stability of both sides of ESM and ESM-HA composites were systematically studied. Results indicated that both sides of ESM exhibited excellent biomimetic mineralisation ability, and the hydrophilicity and thermal stability of ESM were effectively improved by the introduction of HA. Moreover, in vitro experiments on MC3T3-E1 cells revealed that the inner side of the ESM was more beneficial to cell proliferation and adhesion than the outer side. Remarkably, the proliferation, adhesion and spreading, as well as the alkaline phosphatase (ALP) activity and expression of bone-related genes and proteins (runt-related transcription factor 2, ALP, collagen type I, and osteocalcin) on both sides of ESM-HA composites were significantly higher compared to those of the original ESM. These findings suggested that ESM-HA composites obtained using biomimetic mineralisation could be potential new materials for future bone tissue repair.


Assuntos
Materiais Biomiméticos/farmacologia , Calcificação Fisiológica/efeitos dos fármacos , Casca de Ovo/efeitos dos fármacos , Nanofibras/química , Osteogênese/efeitos dos fármacos , Fosfatase Alcalina/metabolismo , Animais , Calcificação Fisiológica/genética , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Casca de Ovo/ultraestrutura , Regulação da Expressão Gênica/efeitos dos fármacos , Interações Hidrofóbicas e Hidrofílicas , Membranas/efeitos dos fármacos , Membranas/ultraestrutura , Camundongos , Osteogênese/genética , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Água
12.
Carbohydr Polym ; 212: 277-288, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30832858

RESUMO

In order to better utilize and combine the advantages of poly(d,l-lactide) (PDLLA) matrix and natural polysaccharide chitin whiskers (CHWs), needle-like CHWs with chiral nematic liquid crystal characteristics and remarkable moduli were chosen to surface modify PDLLA film. By a vertical coating method, a stable and well-ordered CHWs coating was formed on the surface of the PDLLA film through a polydopamine (PDA) interlayer. The high stability of the well-ordered CHWs coating on the surface of the PDLLA film was confirmed by PBS soaking experiment in terms of the changes of weight and surface morphology of the film. The surface microstructure and composition of the resulting PDLLA-PDA-CHWs film were characterized by field emission scanning electron microscopy (FESEM), atomic force microscope (AFM) and X-ray photoelectron spectroscopy (XPS), and the thickness of the PDLLA, PDA and CHWs layers was determined using FESEM, too. Comparing with traditional PDLLA/CHWs film, prepared by solution blending with optimal content of 5 wt% CHWs, the hydrophilicity and mechanical properties of the PDLLA-PDA-CHWs film were significantly improved owing to the immobilization of a highly ordered CHWs coating. Furthermore, in vitro cell culture showed that the well-ordered CHWs coating had a more significant effect on promoting the adhesion, spreading, proliferation and osteogenic differentiation of MC3T3-E1 cells. All these results showed that the resulting PDLLA-PDA-CHWs film with well-ordered CHWs coating designed through simple and effective approach has promising application in bone repair material field.


Assuntos
Quitina/química , Quitina/farmacologia , Osteogênese/efeitos dos fármacos , Poliésteres/química , Poliésteres/farmacologia , Animais , Fenômenos Biomecânicos/efeitos dos fármacos , Fenômenos Biomecânicos/fisiologia , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/fisiologia , Linhagem Celular , Estabilidade de Medicamentos , Camundongos , Osteogênese/fisiologia
13.
Carbohydr Polym ; 209: 92-100, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30732829

RESUMO

To explore the concentration range of chitin whiskers (CHWs) aqueous suspensions with the liquid crystalline state, and the correlation between liquid crystalline characteristic and rheology of CHWs suspensions, the CHWs aqueous suspensions with different CHWs concentration, pH value, ionic strength and temperature were prepared. The liquid crystalline characteristic and rheological properties of the obtained suspensions were investigated by polarizing microscope and rotational rheometer. The results showed that the liquid crystalline state of the suspensions appeared at the CHWs concentration of 3.5 wt% and disappeared at 22.5 wt%. Increasing the CHWs concentration, pH value and ionic strength, the values of elastic modulus (G') and viscous modulus (G") increased, and the liquid crystal texture basically became more and more regular and obvious. Moreover, the value of G"/G' was always less than 1, suggesting the elastic behavior of CHWs aqueous suspension. Besides, the effect caused by heating on the elastic modulus was irreversible.

14.
J Mech Behav Biomed Mater ; 90: 604-614, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30500698

RESUMO

Polyhedral oligomeric silsesquioxane reinforced poly (L-lactic acid) nanocomposites (PLLA/POSS) were prepared to overcome the insufficient mechanical properties of PLLA. In order to improve the compatibility between the nanofillers and matrix, PLLA chains were grafted onto the POSS nanoparticles via microwave-assisted ring opening polymerization (ROP). Herein, a series of interface-modified polyhedral oligomeric silsesquioxane (POSS-(PLLA)32) nanoparticles with various PLLA tail lengths were synthesized and the influence of the structure and additional amount of POSS nanoparticles on the properties of PLLA based nanocomposites were studied. POSS nanoparticles exhibit effective nucleation activity and lead to a significant improvement in the mechanical strength, thermal stability and biocompatibility of the resulting nanocomposites. The addition of 6 wt% POSS-(PLLA)32 600 shows the optimal mechanical properties owing to has the longest PLLA tail length on POSS core, which possesses the optimal interfacial compatibility between POSS nanoparticles and PLLA. The Young's modulus improved by 57% and the tensile strength increased by 26.5% compared with neat PLLA. Moreover, the introduction of POSS nanoparticles lead to a porous fiber structure when processed by electrospinning and the nanofibrous scaffold effectively promoted cells adhesion and spreading. These results demonstrate the potential applications of the PLLA/POSS nanocomposites in tissue engineering and regenerative medicine.


Assuntos
Materiais Biocompatíveis/química , Fenômenos Mecânicos , Nanocompostos/química , Compostos de Organossilício/química , Poliésteres/química , Células 3T3 , Animais , Materiais Biocompatíveis/farmacologia , Adesão Celular/efeitos dos fármacos , Teste de Materiais , Camundongos , Poliésteres/farmacologia , Propriedades de Superfície , Temperatura
15.
ACS Biomater Sci Eng ; 5(10): 5316-5326, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33455236

RESUMO

In order to study the relationship between the degree of deacetylation of chitin whiskers (CHWs) and their enzymatic degradation properties, in this paper, CHWs were first deacetylated to different degrees by alkali treatment, and the CHWs with the degrees of deacetylation of 17.84, 67.76, and 82.54% was obtained, respectively. Moreover, the partially deacetylated CHWs still maintained good crystallinity and nanoneedle-like morphology. Next, the in vitro degradation behavior of CHWs with different degrees of deacetylation was further studied under the single or synergistic action of lysozyme and lipase (37 °C, pH = 7.4). The results showed that the morphology change of CHWs was more obvious as the degree of deacetylation increased. The mass loss, the crystallinity index at the (110) crystal plane, and the concentration of reducing sugar of the CHWs also increased with the degree of deacetylation. Moreover, the synergistic effect of the two enzymes was more conducive to the degradation process of CHWs than single lysozyme or lipase. The difference in the rate of enzymatic degradation provides an idea for the regulation of the degradation rate of the CHWs.

16.
Mater Sci Eng C Mater Biol Appl ; 93: 319-331, 2018 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-30274064

RESUMO

In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too. Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively. Antibacterial experiment against E. coli and S. aureus indicated that antibacterial activity of the G-C-D-PU fibrous membrane was markedly superior to that of pure PU and C-D-PU fibrous membranes. In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L. Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.


Assuntos
Antibacterianos , Quitina/análogos & derivados , Escherichia coli/crescimento & desenvolvimento , Indóis , Membranas Artificiais , Polímeros , Poliuretanos , Staphylococcus aureus/crescimento & desenvolvimento , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Quitina/química , Quitina/farmacologia , Indóis/química , Indóis/farmacologia , Camundongos , Células NIH 3T3 , Polímeros/química , Polímeros/farmacologia , Poliuretanos/química , Poliuretanos/farmacologia
17.
Mater Sci Eng C Mater Biol Appl ; 81: 280-290, 2017 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-28887974

RESUMO

To synergistically improve the mechanical properties and osteogenic activity of electrospinning poly(l-lactide) (PLLA) membrane, chitosan (CS) nanofibers were firstly introduced to prepare sub-micro and nanofibers interpenetrated PLLA/CS membrane, which was further surface modified with a polydopamine (PDA) layer to obtain PLLA/CS-PDA. Surface morphology, porosity, surface area and hydrophilicity of the obtained fibrous membranes were studied in detail. As compared to pure PLLA, the significant increase in the mechanical properties of the PLLA/CS, and especially of the PLLA/CS-PDA, was confirmed by tensile testing both in dry and wet states. Cells culture results indicated that both the PLLA/CS and PLLA/CS-PDA membranes, especially the latter, were more beneficial to adhesion, spreading and proliferation, as well as up-regulating alkaline phosphate activity and calcium deposition of MC3T3-E1 cells than PLLA membrane. Results suggested there was a synergistic effect of the CS nanofibers and PDA layer on the mechanical properties and osteogenic activity of PLLA membrane.


Assuntos
Nanofibras , Quitosana , Indóis , Poliésteres , Polímeros
18.
Mater Sci Eng C Mater Biol Appl ; 79: 399-409, 2017 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-28629034

RESUMO

In this study, icariin (ICA), one of the main active ingredients of Herba Epimedii for osteogenesis, was applied to functionalize electrospinning poly(l-lactide) (PLLA) fibrous membrane via an intermediate layer of polydopamine (PDA) to obtain enhanced cytocompatibility and osteogenic activity. For this purpose, an array of PDA-coated PLLA fibrous membranes (PLLA-0.5PDA, PLLA-1PDA, PLLA-2PDA, PLLA-5PDA) and ICA-modified PLLA-2PDA fibrous membranes (PLLA-2PDA-10ICA, PLLA-2PDA-20ICA, PLLA-2PDA-40ICA) were successively prepared. Successful modification of PDA and ICA onto PLLA fibrous membranes was verified by field emission scanning electron microscope (FESEM), thermogravimetric analysis (TGA) and X-ray photoelectron spectroscopy (XPS). Besides, the hydrophilicity as well as tensile properties of PLLA fibrous membrane were improved after surface modified with PDA and ICA. In vitro cells culture experiments revealed that the adhesion, proliferation and osteogenic differentiation of MC3T3-E1 cells on the PLLA fibrous membrane were significantly improved by successively immobilized with PDA and ICA. Moreover, the concentration of ICA immobilized on the fibrous membranes has the complicated effects on the MC3T3-E1 cells behavior. The PLLA-2PDA-ICA fibrous membranes with low ICA concentration promoted the cell adhesion and proliferation, but on the contrary, those with high ICA concentration were more beneficial to the enhancement in ALP activity and calcium deposition.


Assuntos
Flavonoides/química , Adesivos , Indóis , Osteogênese , Poliésteres , Polímeros , Propriedades de Superfície
19.
Mater Sci Eng C Mater Biol Appl ; 70(Pt 1): 701-709, 2017 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-27770944

RESUMO

Osteogenesis and angiogenesis play the prominent role in the bone regeneration. In this study, deferoxamine (DFO), an induced agent for osteogenesis and angiogenesis, was modified onto the surface of poly(D,L-lactide) (PDLLA) membrane via a facile and convenient approach based on the self-polymerization of dopamine (DOPA). The surface composition, morphology, hydrophilicity and surface energy of the original and modified PDLLA membranes were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), scanning electronic microscopy (SEM), atomic force microscopy (AFM) and contact angle measurement. The surface roughness and hydrophilicity of the PDLLA membrane were obviously increased by introducing either the single polydopamine (PDOPA) or the dual layers of PDOPA and DFO. In vitro cells culture experiments indicated that both the PDLLA/PDOPA and PDLLA/PDOPA-DFO composite membranes were more beneficial to the attachment, proliferation and spreading of MC3T3-E1 cells and HUVECs compared to the original PDLLA membrane. The PDLLA/PDOPA-DFO membrane was supportive for the proliferation of both MC3T3-E1 cells and HUVECs, and especially for HUVECs. The results suggested that the as-prepared PDLLA/PDOPA-DFO composite can be expected to be used as a promising bone regenerative material with promoted angiogenesis.


Assuntos
Adesivos/farmacologia , Materiais Revestidos Biocompatíveis/farmacologia , Desferroxamina/farmacologia , Células Endoteliais da Veia Umbilical Humana/citologia , Indóis/farmacologia , Membranas Artificiais , Osteoblastos/citologia , Poliésteres/farmacologia , Polímeros/farmacologia , Animais , Proliferação de Células/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Humanos , Interações Hidrofóbicas e Hidrofílicas , Camundongos , Células-Tronco Embrionárias Murinas/citologia , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Espectroscopia Fotoeletrônica , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de Superfície
20.
Carbohydr Polym ; 156: 235-243, 2017 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-27842818

RESUMO

The aim of this study was to provide a convenient surface modification method for polyurethane (PU) membrane and evaluate its influence on hydrophilicity, antibacterial activity and cell functions, which are the most important factors for wound dressings. For this purpose, chitooligosaccharide (COS) was modified onto the surface of PU membrane based on the self-polymerization of dopamine (DOPA). Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU membranes were characterized. Surface roughness and hydrophilicity of the PU membrane were obviously increased by modified with polydopamine (PDOPA) and COS. Antibacterial experiment against Escherichia coli and Staphylococcus aureus indicated that antibacterial activity of PU membrane increased only slightly by modified with PDOPA, but increased significantly by further modified with COS. Cells culture results revealed that COS-functionalized PU membrane is more beneficial to the adhesion and proliferation of NIH-3T3 cells compared to the original and PDOPA-modified PU membranes.


Assuntos
Indóis/farmacologia , Polímeros/farmacologia , Poliuretanos/farmacologia , Adesivos , Animais , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Quitina/análogos & derivados , Quitina/química , Indóis/toxicidade , Camundongos , Células NIH 3T3 , Polímeros/toxicidade , Poliuretanos/toxicidade , Propriedades de Superfície
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