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1.
Environ Int ; 136: 105461, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31931349

RESUMO

BACKGROUND: Organophosphate esters (OPEs) are a class of alternative replacements for polybrominated diphenyl ethers. In vitro and in vivo studies suggested that OPEs may disrupt the homeostasis of sex steroid hormones. However, human evidence in children and adolescents is limited. OBJECTIVES: We conducted a cross-sectional analysis of the associations between OPE biomarkers and sex steroid hormones among children (6-11 years) and adolescents (12-19 years) in the U.S. National Health and Nutrition Examination Survey, 2013-2014. METHODS: Participants aged 6-19 years who had available data on urinary OPE metabolites, serum sex hormones [total testosterone (TT), estradiol (E2)] and sex hormone binding globulin (SHBG) were included (n = 544). Free androgen index (FAI) calculated as TT divided by SHBG and a ratio of TT to E2 (TT/E2) were generated. Five urinary OPE metabolites were examined. A constructed puberty status was defined as either high steroid hormone levels (TT ≥ 50 ng/dL in males and E2 ≥ 20 pg/ml in females) or onset of menarche. Multiple linear regression and weighted quantile sum (WQS) regression analyses stratified by sex-age and sex-puberty-status groups were conducted to examine the associations of OPE metabolites and its mixture with sex hormone levels. RESULTS: After adjusting for covariates, dibutyl phosphate (DBUP) and dibutyl phosphate (DPHP) were significantly inversely associated with TT (or FAI) and E2; DBUP was negatively associated with SHBG; and DPHP was positively associated with SHBG and TT/E2 in female adolescents. In male adolescents, we observed monotonic negative associations of bis(1,3-dichloro-2-propyl) phosphate (BDCPP), DBUP or DPHP with TT (or FAI) and E2, and positive associations of BDCPP and DPHP with SHBG. Among adolescents, the OPEs index was negatively associated with TT [WQS beta = -0.29 (95% confidence interval: -0.51, -0.07) in males and -0.15 (-0.28, -0.01) in females ], FAI [-0.46 (-0.71, -0.2) in males and -0.23 (-0.41, -0.05) in females] and E2 [-0.25 (-0.41, -0.1) in males and -0.33 (-0.59, -0.08) in females], with stronger associations with TT and FAI in males and a slightly stronger association with E2 in females. In addition, the OPEs index presented a comparable positive association with SHBG in both sexes of adolescents. In contrast, significant associations of individual OPE metabolites or OPEs index with sex hormones were sparse in children. Results by sex-puberty status in single pollutant and WQS regression analyses presented a similar pattern, where most of the significant associations were limited to the pubertal individuals. Of note, stronger inverse associations of the OPEs index with TT and FAI remained in pubertal boys. But the association between the OPEs index and E2 was non-significant in pubertal girls, and only in pubertal boys did the OPEs index show a significant and stronger inverse association with E2. CONCLUSIONS: Exposure to OPEs, either individually or as a mixture, was associated with decreased levels of certain sex steroid hormones (TT, FAI, and E2) and increased levels of SHBG in adolescents or pubertal individuals, with the associations presenting somewhat sex-dependent pattern. However, there is little evidence of the significant associations in children or prepubescent ones. Given the cross-sectional nature of the analysis, our findings need further confirmation.

2.
J Cell Mol Med ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31945804

RESUMO

The specific mechanism of pulmonary arterial hypertension (PAH) remains elusive. The present study aimed to explore the underlying mechanism of PAH through the identity of novel biomarkers for PAH using metabolomics approach. Serum samples from 40 patients with idiopathic PAH (IPAH), 20 patients with congenital heart disease-associated PAH (CHD-PAH) and 20 healthy controls were collected and analysed by ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-HRMS). Orthogonal partial least square-discriminate analysis (OPLS-DA) was applied to screen potential biomarkers. These results were validated in monocrotaline (MCT)-induced PAH rat model. The OPLS-DA model was successful in screening distinct metabolite signatures which distinguished IPAH and CHD-PAH patients from healthy controls, respectively (26 and 15 metabolites). Unbiased analysis from OPLS-DA identified 31 metabolites from PAH patients which were differentially regulated compared to the healthy controls. Our analysis showed dysregulation of the different metabolic pathways, including lipid metabolism, glucose metabolism, amino acid metabolism and phospholipid metabolism pathways in PAH patients compared to their healthy counterpart. Among these metabolites from dysregulated metabolic pathways, a panel of metabolites from lipid metabolism and fatty acid oxidation (lysophosphatidylcholine, phosphatidylcholine, perillic acid, palmitoleic acid, N-acetylcholine-d-sphingomyelin, oleic acid, palmitic acid and 2-Octenoylcarnitine metabolites) were found to have a close association with PAH. The results from the analysis of both real-time quantitative PCR and Western blot showed that expression of LDHA, CD36, FASN, PDK1 GLUT1 and CPT-1 in right heart/lung were significantly up-regulated in MCT group than the control group.

4.
J Clin Lab Anal ; : e23172, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31907990

RESUMO

BACKGROUND: In previous research, we found diabetes rather than obesity was an independent risk factor of breast cancer. However, why diabetes could lead to increased risk of breast cancer patients remains elusive. Long non-coding RNAE330013P06 has been shown to be upregulated in diabetes, and long non-coding RNAs generally promote progression of cancer. METHODS: About 200 specimens of breast patients were obtained in previous clinical trial; 34 samples diagnosed as type 2 diabetes in breast cancer patient were enrolled in this research. Blood samples from 36 patients diagnosed as breast cancer without diabetes; 35 diabetic patients and 35 healthy peoples were obtained as control. All blood samples were measured by quantitative real-time PCR (qRT-PCR). Invasion and migration were tested by Transwell assay. Cell proliferation assay was tested by CCK-8. Protein analysis was determined by Western blot. RESULTS: Compared with breast cancer patients without diabetes, diabetic patients without breast cancer and healthy peoples, LncRNAE330013P06 was upregulated in breast cancer patient with diabetes. Furthermore, of 34 breast patients, high LncRNAE330013P06 expression was significantly associated with family history, tumor-node-metastasis stage and lymph node metastasis. E33 promoted cancer cell growth in vitro via downregulation of P53. CONCLUSION: Upregulation of LncRNAE330013P06 driven by type 2 diabetes is one of the factors which promoted progression of breast cancer.

5.
Sci Rep ; 9(1): 18475, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31811159

RESUMO

A combined anterior and posterior (AP) surgical approach is a popular treatment modality of lumbosacral tuberculosis, but it is often traumatic and complicated. The present study aims to find whether the anterior only approach with the ARCH plate system is less invasive than the AP approach in treating lumbosacral tuberculosis. The ARCH plate system is an innovative anatomic lumbosacral anterior multi-directional locking plate system which was devised with due consideration to the anatomic features of the lumbosacral spine and irregular destruction of involved vertebral endplates. In this retrospective study, 32 patients with lumbosacral tuberculosis underwent surgeries via either the anterior only approach (ARCH group, 18 patients) using the ARCH system or the conventional combined anterior and posterior approach (AP group, 14 patients). American Spinal Injury Association (ASIA) scores, Visual Analogue Scale (VAS) scores, Oswestry Disability Index (ODI), bone union status, ESR, CRP, intervertebral foraminal height between L5 and S1, the vertical height between the anterior upper edge of L5 and S1 vertebral body, lumbosacral angle, and the physiological lordosis of between L1 and S1 from both groups were recorded and compared. All patients were followed up for at least two years. The average duration of operation, blood loss, and length of hospital admission of the ARCH group (154.6 min, 361.1 ml&18.3days) was significantly smaller and shorter(p < 0.001, p < 0.001 & p = 0.008) that those of the AP group(465.5 min, 814.3 ml & 24.6days). The ODI score(p = 0.08, 0.471, 0.06, 0.07, 0.107), the VAS score(p = 0.099, 0.249, 0.073, 0.103, 0.273), the intervertebral foraminal height between L5 and S1(p = 0.826, 0.073, 0.085), L5-S1 height(p = 0.057, 0.234, 0.094), lumbosacral angle(p = 0.052, 0.242, 0.825), and L5-S1 lordosis(p = 0.146, 0.129, 0.053) of both groups showed no significant difference in any of the time points. The anterior only approach using the ARCH system is as effective as the combined anterior and posterior approach and is less traumatic in treating lumbosacral tuberculosis.

6.
J BUON ; 24(5): 1943-1949, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31786859

RESUMO

PURPOSE: To compare the short-term efficacy and safety of laparoscopic precise hepatectomy and conventional open hepatectomy in the treatment of primary hepatocellular cancer. METHODS: 90 patients with primary hepatocellular cancer admitted to our hospital from September 2015 to September 2017 were collected and divided into the laparoscopic precise hepatectomy group (experimental group, n=45) and conventional open hepatectomy group (control group, n=45). The differences in operation time, intraoperative blood loss, postoperative time with drainage tubes, perioperative blood transfusion, postoperative hospital stay, postoperative liver function indicators, postoperative complications and 1-year tumor recurrence rate and survival rate after operation between the two groups were compared. RESULTS: The general clinical features of the two groups of patients were comparable, and there was no perioperative death. The operation time in the experimental group was longer than that in the control group (106.5±26.4 min vs. 95.2±21.3 min, p=0.028). The intraoperative blood loss in the experimental group was less than that in the control group (204.3±34.9 mL vs. 285.2±39.9 mL, p<0.001). The postoperative time with drainage tubes (6.2±1.7 days vs. 7.1±2.1 days, p=0.028) and postoperative hospital stay (8.1±2.5 days vs. 12.2±3.3 days, p<0.001) in the experimental group were significantly shorter than those in the control group. The levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the experimental group were significantly lower than those in the control group (p<0.05), while the levels of albumin (ALB) and total bilirubin (TBIL) in the former were obviously higher than those in the latter (p<0.05). The incidence rate of postoperative complications in the experimental group (15.6%) was evidently lower than that in the control group (35.6%; p=0.030). There was no significant difference in the 1-year recurrence rate between the two groups (p=0.086), and the 1-year survival rate in the experimental group was notably higher than in the control group (p=0.019). CONCLUSION: Laparoscopic precise hepatectomy for the treatment of primary hepatocellular cancer significantly reduces intraoperative blood loss, shortens hospital stay, causes less trauma to patients and liver function damage, improves the 1-year survival rate, and has a lower incidence rate of complications than the traditional laparotomy, so it is worthy of clinical promotion.

7.
Sci Rep ; 9(1): 18683, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31822729

RESUMO

Time perception is an important ability that is related closely to humans' and animals' daily activities. It can be distorted by various emotional states. In human studies, experimental pain has been shown to prolong the perception of time. However, related animal studies are lacking. In this study, we used a temporal bisection task to investigate how acute inflammatory pain (induced by hind-paw formalin injection) and chronic neuropathic pain [induced by spinal nerve ligation (SNL)] affected time perception in rats. Rats were trained to recognize "short" (1200-ms) and "long" (2400-ms) anchor-duration pure tones and were rewarded for corresponding lever presses. During testing, rats perceived a series of intermediate-duration and anchor-duration pure tones, and selected levers corresponding to the "short" and "long" tones. After formalin injection, rats gave more "long" lever-press responses than after saline injection. The point of subjective equality after formalin injection also increased, suggesting that formalin-induced acute pain extended time perception. In contrast, rats that had undergone SNL gave fewer "long" lever-press responses compared with the sham surgery group. This animal study suggests that formalin-induced pain and neuropathic pain may have different effects on time perception.

8.
Sci Rep ; 9(1): 18726, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31822749

RESUMO

Depressed individuals are biased to perceive, interpret, and judge ambiguous cues in a negative/pessimistic manner. Depressed mood can induce and exacerbate these biases, but the underlying mechanisms are not fully understood. We theorize that depressed mood can bias ambiguity processing by altering one's subjective emotional feelings (e.g. pleasantness/unpleasantness) of the cues. This is because when there is limited objective information, individuals often rely on subjective feelings as a source of information for cognitive processing. To test this theory, three groups (induced depression vs. spontaneous depression vs. neutral) were tested in the Judgement Bias Task (JBT), a behavioral assay of ambiguity processing bias. Subjective pleasantness/unpleasantness of cues was measured by facial electromyography (EMG) from the zygomaticus major (ZM, "smiling") and from the corrugator supercilii (CS, "frowning") muscles. As predicted, induced sad mood (vs. neutral mood) yielded a negative bias with a magnitude comparable to that in a spontaneous depressed mood. The facial EMG data indicates that the negative judgement bias induced by depressed mood was associated with a decrease in ZM reactivity (i.e., diminished perceived pleasantness of cues). Our results suggest that depressed mood may bias ambiguity processing by affecting the reward system.

9.
Huan Jing Ke Xue ; 40(12): 5640-5648, 2019 Dec 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854637

RESUMO

A combined Hydrus-1D and groundwater dilution model was employed to determine the risk control value and risk control volume of soil in correspondence of an electroplating workshop located in the Pearl River Delta. In particular, we considered the risk control value and risk control volume of soil in relation to characteristic chromium (Ⅵ) and nickel pollutants, which can affect the quality of groundwater. Based on the absorption of pollutants by the soil in the vadose zone, we obtained soil risk control values of 41.6 mg·kg-1 and 619.1 mg·kg-1 for chromium (Ⅵ) and nickel, respectively. These values were 10 and 45 times greater than those obtained from theoretical calculations (based on the soil/water partition equation combined with the groundwater dilution model), while the soil risk control volumes of chromium (Ⅵ) and nickel were 1804 m3 and 44590 m3, respectively. The soil risk control values calculated through the soil/water partition equation combined with the groundwater dilution model tend to be excessively conservative for the contaminated sites of the Pearl River Delta (characterized by a shallow buried depth of the underground water level and a close hydraulic connection); hence, it is necessary to carry out further hydrogeological surveys and fully consider the migration process of pollutants in the vadose zone. We conclude that the combined Hydrus-1D and groundwater dilution model for groundwater protection can be used to determine the soil risk control value, potentially saving the costs involved in later remediation and risk control.

10.
BMC Bioinformatics ; 20(Suppl 25): 692, 2019 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-31874603

RESUMO

BACKGROUND: The Copy Number Alterations (CNAs) are discovered to be tightly associated with cancers, so accurately detecting them is one of the most important tasks in the cancer genomics. A series of CNAs detection methods have been proposed and new ones are still being developed. Due to the complexity of CNAs in cancers, no CNAs detection method has been accepted as the gold standard caller. Several evaluation works have made attempts to reveal typical CNAs detection methods' performance. Limited by the scale of evaluation data, these different comparison works don't reach a consensus and the researchers are still confused on how to choose one proper CNAs caller for their analysis. Therefore, it needs a more comprehensive evaluation of typical CNAs detection methods' performance. RESULTS: In this work, we use a large-scale real dataset from CAGEKID consortium to evaluate total 12 typical CNAs detection methods. These methods are most widely used in cancer researches and always used as benchmark for the newly proposed CNAs detection methods. This large-scale dataset comprises of SNP array data on 94 samples and the whole genome sequencing data on 10 samples. Evaluations are comprehensively implemented in current scenarios of CNAs detection, which include that detect CNAs on SNP array data, on sequencing data with tumor and normal matched samples and on sequencing data with single tumor sample. Three SNP based methods are firstly ranked. Subsequently, the best SNP based method's results are used as benchmark to compare six matched samples based methods and three single tumor sample based methods in terms of the preprocessing, recall rate, Jaccard index and segmentation characteristics. CONCLUSIONS: Our survey thoroughly reveals 12 typical methods' superiority and inferiority. We explain why methods show specific characteristics from a methodological standpoint. Finally, we present the guiding principle for choosing one proper CNAs detection method under specific conditions. Some unsolved problems and expectations are also addressed for upcoming CNAs detection methods.

11.
J Clin Neurosci ; 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31843435

RESUMO

An innovative concept was proposed to treat the lumbar degenerative diseases with full-endoscopic translaminar lumbar interbody fusion (Endo-TLIF) and the efficacy was evaluated. A total of 44 patients from January 2017 to October 2018 were enrolled, including 20 patients undergoing full-endoscopic TLIF and 24 patients undergoing standard open TLIF. The Visual Analogue Scale (VAS) score, Oswestry Dability Index (ODI) and intervertebral fusion were observed in both groups at the last follow-up. The height and width of intervertebral foramen and height of intervertebral space were evaluated via imaging. Mean follow-up time for all patients 13.2 ±â€¯3.2 months. The average operation time was 184.3 ±â€¯70.6 min in experimental group and 191.1 ±â€¯32.4 min in control group. The amount of bleeding was 38.5 ±â€¯19.5 mL and 214.6 ±â€¯61.6 mL. The VAS score was 4.9 ±â€¯1.0 points in experimental group and 4.8 ±â€¯1.0 points in control group before operation, and 0.8 ±â€¯0.6 points and 0.8 ±â€¯0.7 points at the last follow-up. The ODI was 83.5 ±â€¯7.8% and 87.8 ±â€¯5.9% before operation, and 9.9 ±â€¯3.6% and 9.3 ±â€¯3.7% at the last follow-up. The height and width of intervertebral foramen and height of intervertebral space in both groups were significantly improved (p < 0.05). The fusion rate at the last follow-up were 95% (19 cases) in experimental group and 92% (22 cases) in control group. Full-endoscopic TLIF is feasible in the treatment of single-segment lumbar degenerative diseases, which is characterized by small trauma, quick recovery and low cost. However, the large-sample long-term follow-up is needed to further evaluate its clinical efficacy and safety.

12.
Prenat Diagn ; 39(13): 1273-1282, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31671222

RESUMO

OBJECTIVE: To analyze the fetal fraction, fetal sex, and chromosomal aneuploidy in multiple pregnancies using noninvasive prenatal testing (NIPT). METHOD: A total of 362 pregnant women including 203 singleton pregnancies, 69 twins, and 90 higher-order multiple pregnancies were recruited. Fetal fractions estimated by size ratio-based and Y chromosome-based approaches in singleton pregnancies with male fetus were used as source data to establish the model. The model was then applied to multiple pregnancies for fetal fraction estimation. By comparing the fetal fractions estimated by size ratio to those estimated by Y chromosome or autosomal chromosomes, fetal sex and chromosomal aneuploidy can be analyzed. RESULTS: The size ratio-based approach has been well established in estimating fetal fractions for twin and higher-order multiple pregnancies. Fetal fraction had a positive correlation with gestational age in twin and triplet pregnancies. Fetal sex was determined with accuracies of 98.6% (95% CI, 92.19%-99.96%) in twins and 97.6% (95% CI, 91.76%-99.71%) in triplet pregnancies. Four trisomy 21, one trisomy 18, and one trisomy 13 cases were detected by NIPT. Two trisomy 21 singleton pregnancies and one trisomy 21 twin pregnancy were confirmed by karyotyping. CONCLUSION: Fetal sex and chromosomal aneuploidy in multiple pregnancies can be determined using NIPT.

14.
Pancreatology ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31771905

RESUMO

BACKGROUND: Pancreatic neuroendocrine tumors (pNET) are rare and heterogeneous. New biomarkers are needed for better predicting the prognosis and for providing individualized treatment. Versican (VCAN) plays an important role in tumorigenesis. Therefore, we plan to investigate the role of VCAN in pNET prognosis. METHOD: The clinical and pathological data of pNET patients who underwent surgery between 2005 and 2010 were collected and evaluated. Radiologic tumor assessments with contrast computed tomography or magnetic resonance imaging were performed at baseline and follow up. The radiologic response was classified according to the RECIST 1.1 criteria. VCAN expression was assessed by immunohistochemical staining (IHC). RESULT: Among 155 pNET patients, 112 (72.3%) pNET patients were VCAN positive, and 43 (27.7%) were negative. Positive expression of VCAN in pNET was significantly associated with a longer disease-free survival (DFS) compared with VCAN negative pNET (p = 0.038, HR 0.462, 95% CI 0.218-0.978). Subgroup analysis showed that VCAN positive expression was associated with a longer DFS in the G1 subgroup (p = 0.031, HR 0.124, 95% CI 0.013-1.193), the tumor size>2 cm subgroup (p = 0.047, HR 0.458, 95% CI 0.207-1.012) and the NF-pNET subgroup (p = 0.003, HR 0.274, 95% CI 0.112-0.673). Multivariable analysis showed that VCAN negative expression, G2 and tumor size>2 cm were independent factors of poor prognosis of pNET (p = 0.041, p < 0.001, p = 0.008, respectively). CONCLUSION: Our data indicate that VCAN positive expression may serve as an independent factor of predicting DFS in pNET; its expression in pNET tissues was correlated with a longer DFS.

15.
Medicine (Baltimore) ; 98(45): e17935, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31702680

RESUMO

BACKGROUND: Recently, many kinds of cages for cervical fusion have been developed to avoid the related complications caused by tricortical iliac crest graft. The existing literature has reported the excellent clinical efficacy and superior fusion rate. However, various types of cages have their own disadvantages. Which bone graft material is the best choice for cage with the fewest complications? At present, there is still no conclusion. METHODS: By reviewing patients with 1 to 2-level cervical degenerative disease in our hospital with a novel cage made of allograft or polyetheretherketone (PEEK), we evaluated the efficacy and reliability of the new cage in anterior cervical discectomy and fusion (ACDF). From 2015 to 2016, a prospective review of 58 and 49 consecutive cases with spondylotic radiculopathy or myelopathy undergoing ACDF using allograft (group A) and PEEK (group B) cage were performed. The follow-up ranged from 12 to 40 months. Intraoperative index, clinical outcome and complications were recorded. Radiographs evaluated segmental and overall cervical lordosis, the height of the intervertebral space, interbody height ratio (IHR), cage positioning, and fusion state. RESULTS: A total of 134 cages were implanted. Compared to preoperatively, the visual analog scale (VAS) and neck disability index (NDI) were reduced postoperatively without any change during the subsequent follow-up in both groups. There was no migration or extrusion of the cages at the latest follow-up. There were 2 and 4 patients suffering dysphagia respectively. In both groups, the intervertebral height, IHR, segmental and overall cervical lordosis were significantly greater than pre-operation (P < .05) and were maintained at the last follow-up, but were not statistically significant (P > .05). The allograft group achieved a fusion rate of 100% (58/58) according to CT scans at 3 months post-operation, while PEEK group was 91.8% (45/49), which reached 95.9% (47/49) at 6 months and 100% at 12 months. In addition, the fusion state was maintained in all patients at the last follow-up. CONCLUSION: Our data showed that the new allograft cage is superior to the PEEK cage in providing a high fusion rate and fewer complications after 1-level and 2-level ACDF procedures. It may represent an excellent alternative to other cages.


Assuntos
Aloenxertos/transplante , Discotomia/métodos , Fixadores Internos , Degeneração do Disco Intervertebral/cirurgia , Cetonas/uso terapêutico , Polietilenoglicóis/uso terapêutico , Fusão Vertebral/métodos , Adulto , Feminino , Humanos , Degeneração do Disco Intervertebral/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Ensaios Clínicos Controlados não Aleatórios como Assunto , Próteses e Implantes , Resultado do Tratamento
16.
Exp Brain Res ; 237(12): 3351-3362, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31720762

RESUMO

Traumatic brain injury (TBI) is a serious health problem in the world. However, little is known about the pathogenesis and molecular mechanisms of TBI. Here, we show that TBI activates neuregulin 1 (NRG1)-ErbB4 signaling, with an increased expression of NRG1 and ErbB4 in the traumatic region. Specifically knocking out ErbB4 in parvalbumin-positive (PV+) interneurons exacerbates motor function deficits in mice after TBI. Consistently, PV-ErbB4-/- mice showed larger necrotic area and more edema when compared with PV-ErbB4+/+ mice. Replenishment of NRG1 through intranasal application of the recombinant protein in PV-ErbB4+/+ mice enhanced neurological function. Moreover, using an in vitro neuronal culture system, we found that NRG1-ErbB4 signaling protects neurons from glutamate-induced death, and such protective effects could be diminished by GABA receptor antagonist. These results indicate that NRG-ErbB4 signaling protects cortical neurons from TBI-induced damage, and such effect is probably mediated by promoting GABA activity. Taken together, these findings unveil a previously unappreciated role for NRG1-ErB4 signaling in preventing neuronal cell death during functional recovery after TBI.

17.
Orthop Surg ; 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31568641

RESUMO

OBJECTIVE: To evaluate the efficacy of three surgical approaches for the treatment of cervicothoracic tuberculosis. METHODS: This is a multicenter retrospective study. We analyzed 74 patients with cervicothoracic tuberculosis who were treated in six institutions between January 2000 and January 2015. There were 37 male and 37 female patients, with an average age of 24 years (range, 5-62 years). The operative method was selected according to the indications. A total of 33 patients underwent one-stage anterior surgery (group A); 16 underwent a combined anterior and posterior surgery (group B) and 25 underwent one-stage posterior surgery (group C). Clinical outcomes, laboratory indexes, and radiological results were analyzed. RESULTS: All cases were followed up for approximately 36-96 months post-surgery (average, 39 months). At the last follow-up, patients in all three groups had achieved bone fusion, with pain relief and neurological recovery. No major vessel and nerve injuries were found during the operation. There were significant differences before and after treatment for visual analogue scale (VAS), neck disability index (NDI), and Japanese Orthopedic Association (JOA) score (P < 0.001). Three surgical strategies significantly improved kyphosis (P < 0.001). CONCLUSION: The choice of operation for cervicothoracic tuberculosis should be selected based on the pathological changes, scope, and general physical condition of the patient. The indication for a posterior approach is narrow and it should be used selectively. The combined anterior and posterior approach involved a longer operating time, larger blood loss, and greater trauma, and also required a higher level of surgical skill. Therefore, the indications for this approach should be strictly controlled. Anterior approach surgery for the treatment of cervicothoracic tuberculosis showed excellent efficacy and fewer complications.

18.
Neurol Res ; 41(12): 1051-1058, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31613201

RESUMO

Objective: To observe the clinical effect of anterior debridement, decompression, bone grafting, and instrumentation for cervical spinal tuberculosis in four hospitals. Materials and Methods: This research retrospectively analyzed 146 patients with cervical spinal tuberculosis who were treated by anterior debridement, decompression, bone grafting, and instrumentation in four institutions between January 2000 and January 2015. There were 68 males and 78 females with an average age of 31.32 ± 11.69 years. All patients received chemotherapy for 18 months after surgery, and fixed by brace for 3 months. Clinical outcome, laboratory indexes and radiological results were analyzed to evaluate the efficacy of anterior approach surgery in the treatment of cervical spinal tuberculosis. Results: All cases were followed up about 18 to 52 months later (average 24 months). At the last follow-up, all patients obtained bone fusion, pain relief and neurological recovery. There was no recurrence in any of the patients, and no complications related to internal fixation. There were statistically significant differences before and after treatment in terms of Visual analog scale (VAS), Neck disability index (NDI) and Japanese Orthopedic Association (JOA)(P < 0.05). During the last follow-up examination, in 83 patients with neurological deficit, 78 patients improved. The kyphosis was significantly improved postoperatively (P < 0.05). At the last follow-up, the Cobb angle had some degree of correction loss, but the difference was not statistically significant. Conclusion: Our study suggests that one-stage anterior debridement, decompression, bone grafting, and instrumentation are safe and effective methods in the surgical management of cervical spinal tuberculosis. Abbreviation: VAS: Visual Analog Scale; JOA: Japanese Orthopaedic Association; NDI: Neck Disability Index; ESR: Erythrocyte Sedimentation Rate; ASIA: American Spinal Injury Association; TB: Tuberculosis.

19.
Int J Equity Health ; 18(1): 152, 2019 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-31615528

RESUMO

BACKGROUND: Health equity is a multidimensional concept that has been internationally considered as an essential element for health system development. However, our understanding about the root causes of health equity is limited. In this study, we investigated the historical roots and seminal works of research on health equity. METHODS: Health equity-related publications were identified and downloaded from the Web of Science database (n = 67,739, up to 31 October 2018). Their cited references (n = 2,521,782) were analyzed through Reference Publication Year Spectroscopy (RPYS), which detected the historical roots and important works on health equity and quantified their impact in terms of referencing frequency. RESULTS: A total of 17 pronounced peaks and 31 seminal works were identified. The first publication on health equity appeared in 1966. But the first cited reference can be traced back to 1801. Most seminal works were conducted by researchers from the US (19, 61.3%), the UK (7, 22.6%) and the Netherlands (3, 9.7%). Research on health equity experienced three important historical stages: origins (1800-1965), formative (1966-1991) and development and expansion (1991-2018). The ideology of health equity was endorsed by the international society through the World Health Organization (1946) declaration based on the foundational works of Chadwick (1842), Engels (1945), Durkheim (1897) and Du Bois (1899). The concept of health equity originated from the disciplines of public health, sociology and political economics and has been a major research area of social epidemiology since the early nineteenth century. Studies on health equity evolved from evidence gathering to the identification of cost-effective policies and governmental interventions. CONCLUSION: The development of research on health equity is shaped by multiple disciplines, which has contributed to the emergence of a new stream of social epidemiology and political epidemiology. Past studies must be interpreted in light of their historical contexts. Further studies are needed to explore the causal pathways between the social determinants of health and health inequalities.

20.
Mol Brain ; 12(1): 85, 2019 10 24.
Artigo em Inglês | MEDLINE | ID: mdl-31651333

RESUMO

Myelin of the central nervous system exhibits strong plasticity, and skill learning exercise promotes oligodendrogenesis and adaptive myelination. Increasing evidence shows that brain structures and functions are affected by physical activity. However, the impact of voluntary physical activity on central myelination and its underlying mechanism remains unclear. The present study aimed to investigate the effect of voluntary wheel running (VWR) on central oligodendrogenesis and adaptive myelination in mice. Adult C57BL/6 J mice were placed in running wheels and allowed for voluntary running 2 weeks. Myelin levels in the central nervous system were detected using western blotting, qRT-PCR, immunohistochemical staining, and electron microscopy. Oligodendrocyte precursor cells (OPCs) and oligodendrocytes (OLs) were detected using immunohistochemical staining and 5-bromo-2-deoxyuridine (BrdU) assays. Motor abilities of the animals were examined using open-field, rotarod running, and beam-walking behavioral paradigms. Vital molecules of Wnt signaling were detected, and the involvement of such molecules was verified using in vitro culture of OPCs. Our results showed that VWR significantly enhanced the myelination in the motor cortex. VWR promoted the proliferation and differentiation of OPCs, and the maturation of OLs. The VWR-regulated myelination was associated with the improved motor skill and decreased mRNA level of Wnt3a/9a, whereas stimulation of Wnt signaling pathway with Wnt3a or Wnt9a suppressed OPCs proliferation and differentiation in vitro. The present study demonstrated that physical activity is highly efficient at promoting myelination in the motor cortex, by enhancing the proliferation of OPCs and accelerating the generation of myelin, providing a step forward in understanding the beneficial effects of physical activity on central myelination and its underlying mechanism.

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