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1.
Clin Rehabil ; 33(9): 1479-1491, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31081365

RESUMO

OBJECTIVE: The aim of this study was to validate a novel pictorial-based Longshi Scale for evaluating a patient's disability by healthcare professionals and non-professionals. DESIGN: Prospective study. SETTING: Rehabilitation departments from a grade A, class 3 public hospital, a grade B, class 2 public hospital, and a private hospital and seven community rehabilitation centers. SUBJECTS: A total of 618 patients and 251 patients with functional disabilities were recruited in a two-phase study, respectively. MAIN MEASURES: Outcome measure: pictorial scale of activities of daily living (ADLs, Longshi Scale). Reference measure: Barthel Index. The Spearman correlation coefficient was used to analyze the validity of Longshi Scale against Barthel Index. RESULTS: In phase 1 study, from March 2016 to August 2016, the results demonstrated that the Longshi Scale was both reliable and valid (intraclass correlation coefficient based on two-way random effect (ICC2,1) = 0.877-0.974 for intra-rater reliability; ICC2,1 = 0.928-0.979; κ = 0.679-1.000 for inter-rater reliability; intraclass correlation coefficient based on one-way random effect (ICC1,1) = 0.921-0.984 for test-retest reliability and Spearman correlation coefficient = 0.836-0.899). In the second phase, in March 2018, results further demonstrated that the Longshi Scale had good inter-rater and intra-rater reliability among healthcare professionals and non-professionals including therapists, interns, and personal care aids (ICC1,1 = 0.822-0.882 on Day 1; ICC1,1 = 0.842-0.899 on Day 7 for inter-rater reliability). In addition, the Longshi Scale decreased assessment time significantly, compared with the Barthel Index assessment (P < 0.01). CONCLUSION: The Longshi Scale could potentially provide an efficient way for healthcare professionals and non-professionals who may have minimal training to assess the ADLs of functionally disabled patients.

2.
Biomed Res Int ; 2019: 2561828, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30941359

RESUMO

The changes of brain metabolism in mice after injection of ginseng glycoproteins (GPr) were analyzed by gas chromatography mass spectrometry- (GC/MS-) based metabolomics platform. The relationship between sedative and hypnotic effects of ginseng glycoproteins and brain metabolism was discussed. Referring to pentobarbital sodium subthreshold test, we randomly divided 20 mice into two groups: control and ginseng glycoproteins group. The mice from the control group were treated with normal saline by i.p and GPr group were treated with 60 mg/kg of GPr by i.p. The results indicated that GPr could significantly improve the sleep quality of mice. Through multivariate statistical analysis, we found that there were 23 differential metabolites in whole brain tissues between the control group and the GPr group. The pathway analysis exhibited that GPr may be involved in the regulation of the pathway including purine metabolism, nicotinate and nicotinamide metabolism, glycine, serine and threonine metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, and steroid hormone biosynthesis. This work is helpful to understand the biochemical mechanism of GPr on promoting sleep and lay a foundation for further development of drugs for insomnia.


Assuntos
Glicoproteínas/farmacologia , Metabolômica/métodos , Panax/química , Sono/efeitos dos fármacos , Animais , Análise Discriminante , Cromatografia Gasosa-Espectrometria de Massas , Análise dos Mínimos Quadrados , Masculino , Metaboloma , Camundongos , Pentobarbital/farmacologia , Análise de Componente Principal , Extratos de Tecidos/química
3.
Molecules ; 23(6)2018 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-29857514

RESUMO

Panax ginseng is well known for its medicinal functions. As a class of important compound of ginseng, ginsenoside is widely studied around the world. In addition, ginseng glycopeptides also showed good biological activity, but researches in this field are rarely reported. In this study, ginseng glycopeptides (Gg) were first prepared from Panax ginseng by reflux extracted with 85% ethanol and the following purification with Sephadex G-15 column. Then, the inflammatory pain models induced by carrageenan and the rat pain models induced by Faure Marin were established for research on mechanism of analgesic activities. It is showed that Gg had an obvious inhibiting effect on inflammation and a significant reduction on the Malondialdehyde (MDA) of inflammatory foot tissue. And there were significant differences between moderate to high dose of Gg and model group in Interleukin 1ß (IL-1ß), Interleukin 2 (IL-2), Interleukin 4 (IL-4), Tumor necrosis factor α (TNF-α) and Histamine. The two models can be preliminarily determined that the analgesic effect of Gg may be peripheral, which mechanism may be related to the dynamic balance between proinflammatory cytokines (TNF-α, IL-1ß) and anti-inflammatory cytokines (IL-2, IL-4, and Interleukin 10 (IL-10)). A series of methods were used to study Gg in physical-chemical properties and linking mode of glycoside. The high-resolution mass spectrometry was used for identification of the structure of Gg. Moreover, the structure of 20 major Gg were investigated and identified. The structural analysis of Gg was benefit for the next study on structure-activity relationship.


Assuntos
Analgésicos/química , Analgésicos/farmacologia , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Glicopeptídeos/química , Glicopeptídeos/farmacologia , Panax/química , Animais , Carragenina/química , Carragenina/farmacologia , Citocinas/metabolismo , Modelos Animais de Doenças , Mediadores da Inflamação/metabolismo , Masculino , Metilação , Estrutura Molecular , Dor/etiologia , Manejo da Dor , Ratos , Relação Estrutura-Atividade , Espectrometria de Massas em Tandem
4.
J Pharm Pharmacol ; 70(8): 1048-1058, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29770446

RESUMO

OBJECTIVES: To investigate the antitumour property of tetrandrine by inducing autophagy and apoptosis in human gastric cancer cells, and to explore the potential molecular mechanisms. METHODS: The antitumour activity of tetrandrine was assessed through MTT assay. Apoptosis was measured by flow cytometry and microscopic examination of cellular morphology. The mitochondrial membrane potential was detected by staining with Rh-123. Induction of autophagy was monitored by transmission electron microscopy observation, using GFP-LC3 transfection. KEY FINDINGS: The results revealed that tetrandrine exhibits significant antitumour activity against gastric human cancer cell and the antigastric tumour activity was depended on inducing autophagy and apoptosis through upregulating the apoptosis-related protein (cleaved PARP, cleaved caspase-3 and cleaved caspase-9) and autophagy-related protein (Beclin-1, LC3-II and p62), and decreasing the phosphorylation of AKT/mTOR, PS6K and P-4EBP1. Adding the inhibitor of autophagy, 3-MA or Baf-A1, increased the viability of tetrandrine-exposed gastric cancer cells, which confirmed the role of autophagy played in the gastric cancer cell death induced by tetrandrine. CONCLUSIONS: These results demonstrated that the antitumour effects of tetrandrine by inducing autophagy and apoptosis involving Akt/mTOR pathway. Thus, tetrandrine may be a promising lead compound to be further developed in future for cancer therapy.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Benzilisoquinolinas/farmacologia , Proliferação de Células/efeitos dos fármacos , Neoplasias Gástricas/patologia , Antineoplásicos Fitogênicos/isolamento & purificação , Benzilisoquinolinas/isolamento & purificação , Técnicas de Cultura de Células , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Humanos , Estrutura Molecular , Proteínas Proto-Oncogênicas c-akt/metabolismo , Stephania tetrandra/química , Serina-Treonina Quinases TOR/metabolismo
5.
Int J Biol Macromol ; 113: 607-615, 2018 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-29408615

RESUMO

The root of Panax ginseng C. A. Mey (Araliaceae) has medicinal value in complex system of Traditional Chinese medicines for its use in improving cognitive function. A glycoproteins named PGL-1 was extracted from ginseng which subjected to through a macroporous resin, hollow-fiber ultrafiltration and dialyzed. The glycoproteins has a molecular weight in the range from 0.4 to 4.4kDa, with an average molecular mass of 1.6kDa. HPLC analysis revealed that the compositions of glycoproteins included fucose, mannose, rhamnose, glucose, galacturonic acid, N-acetylglucosamine and N-acetylgalactosamine. Glycan of PGL-1 has a backbone of →4)-Rha-(1→, →4)-Fuc -(1→, →6)-Gal-(1→, →4)-GalA-(1→, →4)-GlcNAc-(1→ and →4)-GalNAc-(1→,and (→3,6)-Man-(1→) was distributed in branches. The (1→)-Fuc, (1→)-Glc and (1→)-GlcNAc or (1→)-GalNAc were regarded as a terminal residue. The Morris water maze test revealed that the PGL-1 can effectively alleviate the memory impairment symptoms of rats induced by Aß25-35. All dose groups showed significant activity of protective effect on apoptosis SH-SY5Y induced by Aß25-35, and obviously inhibited the S phase arrest. Compared with Aß25-35 treatment alone, a significant reduction in NO concentration and NOS activity was detected in cells co-administered with glycoproteins. Thus, glycoproteins derived from ginseng might be a promising anti-AD reagent.


Assuntos
Glicoproteínas/farmacologia , Fármacos Neuroprotetores/farmacologia , Panax/química , Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/toxicidade , Animais , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Ciclo Celular/efeitos dos fármacos , Ciclo Celular/efeitos da radiação , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Feminino , Humanos , Masculino , Memória/efeitos dos fármacos , Memória/efeitos da radiação , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase/metabolismo , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/toxicidade , Agregados Proteicos , Ratos , Ratos Wistar
6.
J Ethnopharmacol ; 148(3): 946-50, 2013 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-23747537

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The root of Panax ginseng C.A. Mey has various beneficial pharmacological effects. The present study aimed to evaluate the analgesic activities of glycoproteins from the root of Panax ginseng C.A. Mey in mice. MATERIALS AND METHODS: Glycoproteins were isolated and purified from the root of Panax ginseng C.A. Mey. Physicochemical properties and molecular mass were determined by chemical assay and HPLC. Acetic acid-induced writhing and hot-plate tests were employed to study the analgesic effect of glycoproteins and compared with that of aspirin or morphine. The locomotor activity was tested in mice by using actophometer. RESULTS: Four glycoproteins were obtained. The glycoproteins which protein content was the highest (73.04%) displayed dose-dependent analgesic effect. In writhing test, the glycoproteins significantly inhibited writhes (P<0.001) at the dose of 20 mg/kg by intraperitoneal injection. In hot-plate test, only at the dose of 20 mg/kg prolong the hot-plate latency (P<0.05, at 30 min). In the locomotor activity test, the glycoproteins were significant decrease of motility counts at the dose of 20 and 40 mg/kg. CONCLUSION: These findings collectively indicate that the glycoproteins from the root of Panax ginseng C.A. Mey exhibited significant analgesic activities and the proteins were the active site, providing evidence for its pharmacal use.


Assuntos
Analgésicos/uso terapêutico , Glicoproteínas/uso terapêutico , Dor/tratamento farmacológico , Panax , Ácido Acético , Analgésicos/isolamento & purificação , Analgésicos/farmacologia , Animais , Feminino , Glicoproteínas/isolamento & purificação , Glicoproteínas/farmacologia , Temperatura Alta , Masculino , Camundongos , Morfina/farmacologia , Atividade Motora/efeitos dos fármacos , Dor/induzido quimicamente , Dor/fisiopatologia , Fitoterapia , Extratos Vegetais/química , Raízes de Plantas
7.
Molecules ; 17(4): 3609-17, 2012 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-22447024

RESUMO

The body of tremella were decocted with water, and hydrolyzed with 0.1 mol/L hydrochloric acid for different times, giving tremella polysaccharides with six molecular mass values. The structures of all the tremella polysaccharides had non-reducing terminals of ß-D-pyranglucuronide, the backbone was composed of (1 → 3)-linked ß-D-manno-pyranoside, and the side chain composed of (1 → 6)-linked ß-D-xylopyranoside was attached to the C(2) of the backbone mannopyranoside. Immunomodulatory effect studies indicated that tremella polysaccharides increased the counts of leukocytes in the peripheral blood which were significantly lowered by cyclophosphamide, and the lower the molecular mass of the tremella polysaccharide, the better this effect was.


Assuntos
Antineoplásicos Alquilantes/toxicidade , Basidiomycota/química , Ciclofosfamida/toxicidade , Leucopenia/induzido quimicamente , Polissacarídeos/química , Animais , Células da Medula Óssea/efeitos dos fármacos , Feminino , Contagem de Leucócitos , Leucócitos/efeitos dos fármacos , Leucopenia/tratamento farmacológico , Masculino , Metilação , Peso Molecular , Polissacarídeos/administração & dosagem , Polissacarídeos/farmacologia , Ratos
8.
Yao Xue Xue Bao ; 45(7): 813-20, 2010 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-20931776

RESUMO

Heat shock protein 90 is a new target of antitumor drug, the inhibitor of Hsp90 fight against tumor by destroy and degrade the structure of protein. In recent years, looking for Hsp90 inhibitor is not only via structure modifying of natural products, but also via high throughput screening and computer aided drug design to find and synthesize new kinds of Hsp90 inhibitor. Anyway, Hsp90 inhibitor has considered as an important biology target and to pay more and more attention. This review describes recent developments of small molecule Hsp90 inhibitors.


Assuntos
Antineoplásicos/química , Proteínas de Choque Térmico HSP90/antagonistas & inibidores , Adenina/análogos & derivados , Adenina/química , Adenina/farmacologia , Animais , Anisóis/química , Anisóis/farmacologia , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Benzoquinonas/química , Benzoquinonas/uso terapêutico , Catequina/análogos & derivados , Catequina/química , Catequina/farmacologia , Linhagem Celular Tumoral , Cristalização , Proteínas de Choque Térmico HSP90/química , Compostos Heterocíclicos com 2 Anéis/química , Compostos Heterocíclicos com 2 Anéis/farmacologia , Humanos , Lactamas Macrocíclicas/química , Lactamas Macrocíclicas/uso terapêutico , Macrolídeos/química , Macrolídeos/farmacologia , Estrutura Molecular , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Pirazóis/química , Pirazóis/farmacologia , Relação Estrutura-Atividade
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