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1.
J Ethnopharmacol ; 264: 112800, 2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-32224195

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The San Wu Huangqin Decoction (SWHD), which is made from the dried root of Sophora flavescens Aiton (Kushen in Chinese), the dried root of Scutellaria baicalensis Georgi (Huangqin in Chinese), and the dried root tuber of Rehmannia glutinosa (Gaertn.) DC. (Dihuang in Chinese), is a traditional Chinese formula used to treat prolonged fever and inflammatory diseases in clinics and proven to inhibit influenza virus effectively in our previous study. AIM OF THE STUDY: This work was performed to study the regulation of SWHD on inflammation and immune dysfunction induced by the influenza virus and the underlying mechanism in the treatment of SWHD. METHODS: In this study, the influenza virus A/PR/8/34 (H1N1)-infected mouse model was used to investigate the regulation of SWHD on inflammation and immune dysfunction induced by H1N1. The pathological changes, the capacity of proliferation of T and B lymphocytes, the cytotoxicity of natural killer (NK) cells, and the levels of IL-6, TNF-α, IL-1ß, IL-4, and IFN-γ in the serum, bronchoalveolar lavage fluid (BALF), and lung were analyzed. The effects of type 1 T helper cell (Th1) and type 2 T helper cell (Th2) immune responses were discussed indirectly. In addition, the expression levels of p-p65, p65, IKKα/ß, p-IκBα, and IκBα in relation to the NF-κB pathway were measured using Western blot analysis, or immunohistochemical assay. RESULTS: SWHD decreased the pathological changes in lung tissues, promoted the proliferation of T and B lymphocytes, enhanced NK cell activity, and accelerated the phagocytic function of macrophages in H1N1-infected mice. At the same time, SWHD decreased the levels of IL-6, TNF-α, IL-1ß, IFN-γ, and increased the level of IL-4 in the serum, BALF, and lung of model mice. Moreover, the p-p65, p65, and IκBα protein expression levels were inhibited, whereas the p-IκBα protein expression levels were improved in the lungs of H1N1-infected mice. CONCLUSIONS: SWHD can inhibit the replication of the H1N1 virus and reduced the excessive inflammation and immune dysfunction induced by the H1N1 virus in the body. This work provides rich experimental basis for further anti-inflammation research of SWHD and sets the foundation for the development of a viral inflammation drug of traditional Chinese medicine.

2.
Chin J Integr Med ; 24(9): 676-682, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29327126

RESUMO

OBJECTIVE: To investigate the antipyretic mechanism of Herba Ephedrae (Eph)-Ramulus Cinnamomi (RC) herb pair on yeast-induced pyrexia in rats. METHODS: Totally 30 qualified male SD rats were randomly assigned to the normal control (NC) group, the pyrexia model (model) group, the Eph, RC and Eph-RC treatment groups by a random digital table, 6 rats in each group. Each rat received a 20% aqueous suspension of yeast (10 mL/kg) except the NC group. The 3 treatment groups were administered 8.1, 5.4 and 13.5 g/kg Eph, RC and Eph-RC respectively at 5 and 12 h after yeast injection, the NC group and the model groups were administered equal volume of distilled water. Rectal temperatures were measured at 0, 6, 8, 10, 12, 15, 18, 24 and 30 h and urine was collected prior to yeast injection and at 6, 10, 18, 24, 30, and 36 h after yeast injection. Then urine metabolomic profiling by gas chromatography tandem mass spectrometry, coupled with multivariate statistical analysis and pattern recognition techniques were used to explore the antipyretic effects of Eph-RC. Partial least squares discriminate analysis was used to analyze the metabolomics dataset including classification and regression in metabolomics plot profiling. RESULTS: Compared with the NC group, rectal temperatures were significantly higher in the model group (P<0.01), while 3 treatment groups decreased significantly compared with the model group (P<0.05 or P<0.01). Rectal temperatures of Eph-RC-treated rats started to go down at 6 h, and markedly decreased at 8, 12, 15, 18 and 24 h (P<0.05 or P<0.01), while those of the Eph and RC groups had decreased firstly at 8 h and were markedly lower at 12 h (P<0.05 or P<0.01). Seventeen potential biomarkers related to pyrexia were confirmed and identified, including pyruvic acid, L-phenylalanine, L-tyrosine, phenylacetic acid, hippuric acid, succinic acid, citrate and so on. Eight potential alterations of metabolic pathways including phenylalanine metabolism, citrate cycle, tryptophan metabolism, biosynthesis of valine, leucine and isoleucine, were identified in relation to the antipyretic effects of Eph-RC using MetPA software. CONCLUSION: The antipyretic effect of Eph-RC herb pair on yeast-induced pyrexia in rats involved correction of perturbed amino acid, fatty acid, and carbohydrate metabolism according to the metabolic pathway analysis with MetPA.


Assuntos
Antipiréticos/farmacologia , Cinnamomum/química , Medicamentos de Ervas Chinesas/uso terapêutico , Ephedra sinica/química , Febre/tratamento farmacológico , Animais , Febre/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Masculino , Redes e Vias Metabólicas/efeitos dos fármacos , Ratos , Ratos Wistar
3.
Chin J Integr Med ; 2017 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-28755076

RESUMO

OBJECTIVE: To investigate the pharmacokinetic characteristics of three phenylpropanoids (cinnamic acid, cinnamic alcohol and coumarin) in Ramulus Cinnamomi (GZ) and Ramulus Cinnamomi-Ephedrae Herba (MH) herb-couple (GZMH). METHODS: Twelve male Sprague-Dawley rats were randomly and equally divided into the GZ and GZMH herb-couple groups. Blood samples were collected at 0, 0.08, 0.25, 0.5, 0.75, 1.5, 3, 4, 6, 8, 12, 24, 36 and 48 h after oral administration. The three phenylpropanoids in rat plasma were quantified using an ultra-performance liquid-chromatography with tandem mass spectrometry (UPLC-MS/MS) method for pharmacokinetic study. RESULTS: In GZMH group, the area under the curve (AUC), mean retention time (MRT) of cinnamic acid and coumarin were increased significantly (P<0.01, respectively), and biological half-life (t1/2z) was obviously shorter (P<0.05) compared with the GZ group. There were no significant differences in the mean retention time from 0 to ∞ (MRT0-∞), the peak concentration (Cmax), the time to peak (Tmax) and t1/2z, except for AUC and MRT0-t (the mean retention time from 0 to t) of cinnamic alcohol in the GZMH group by comparison to the GZ group (P<0.01, respectively). The AUC, MRT (both P<0.01) and t1/2z (P<0.05) of coumarin were increased significantly, while Cmax, and Tmax were decreased slightly by comparison to the GZ group (P>0.05). CONCLUSIONS: There were statistically significant differences in some pharmacokinetic parameters of the three compounds between GZ and GZMH groups, which meant that MH could affect the absorption and elimination of the three compounds.

4.
Viruses ; 9(4)2017 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-28417913

RESUMO

Shuang-Huang-Lian injectable powder (SHL)-a classical purified herbal preparation extracted from Scutellaria baicalensis, Lonicera japonica, and Forsythia suspense-has been used against human adenovirus III (HAdV3) for many years. The combination herb and its major bioactive compounds, including chlorogenic acid, baicalin, and forsythia glycosides A, are effective inhibitors of the virus. However, no comprehensive studies are available on the antiviral effects of SHL against HAdV3. Moreover, it remains unclear whether the mixture of chlorogenic acid, baicalin, and forsythia glycosides A (CBF) has enhanced antiviral activity compared with SHL. Therefore, a comparative study was performed to investigate the combination which is promising for further antiviral drug development. To evaluate their antivirus activity in parallel, the combination ratio and dose of CBF were controlled and consistent with SHL. First, the fingerprint and the ratio of CBF in SHL were determined by high performance liquid chromatography. Then, a plaque reduction assay, reverse transcription polymerase chain reaction (PCR), real-time polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay (ELISA) were used to explore its therapeutic effects on viral infection and replication, respectively. The results showed that SHL and CBF inhibited dose- and time-dependently HAdV3-induced plaque formation in A549 and HEp-2 cells. SHL was more effective than CBF when supplemented prior to and after viral inoculation. SHL prevented viral attachment, internalization, and replication at high concentration and decreased viral levels within and out of cells at non-toxic concentrations in both cell types. Moreover, the expression of tumor necrosis factor alpha (TNF)-α, interleukin (IL)-1ß, and IL-6 was lower and the expression of interferon (IFN)-γ was higher in both cell types treated with SHL than with CBF. In conclusion, SHL is much more effective and slightly less toxic than CBF.


Assuntos
Adenovírus Humanos/efeitos dos fármacos , Antivirais/farmacologia , Misturas Complexas/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Extratos Vegetais/farmacologia , Adenovírus Humanos/fisiologia , Antivirais/química , Antivirais/isolamento & purificação , Linhagem Celular , Cromatografia Líquida de Alta Pressão , Misturas Complexas/química , Misturas Complexas/isolamento & purificação , Citocinas/antagonistas & inibidores , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Ensaio de Imunoadsorção Enzimática , Forsythia/química , Humanos , Lonicera/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Scutellaria baicalensis/química , Carga Viral , Ensaio de Placa Viral , Ligação Viral/efeitos dos fármacos , Internalização do Vírus/efeitos dos fármacos , Replicação Viral/efeitos dos fármacos
5.
Chin J Integr Med ; 23(2): 138-145, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28265851

RESUMO

OBJECTIVE: To assess the antinociceptive and anti-inflammatory properties of the aqueous extract of Armadillidium vulgare (AV). METHODS: The antinociceptive effect of AV (400, 600 and 800 mg/kg) was investigated in mice using the acetic acid-induced writhing, formalin-induced nociceptive, and hot plate tests. Phlogogen-induced paw edema using carrageenan, dextran, or compound 48/80 as phlogogen was used as inflammatory models to evaluate AV's anti-inflammatory effect. Additionally, the bioactive substances glucosamine (GLcN) and taurine in AV were determined using high-performance liquid chromatography. RESULTS: Oral treatment of the mice with AV (600 and 800 mg/kg) significantly reduced the number of writhes in the acetic acid-induced writhing test (P<0.01) but not the hot plate test (P>0.05). All doses tested significantly inhibited paw-withdrawal during the second phase of the formalin-induced nociceptive model (P<0.01). AV demonstrated a strong anti-inflammatory effect in all those inflammatory models (P<0.05). CONCLUSIONS: AV has antinociceptive and anti-inflammatory effects, providing scientific evidence of the efficacy of its traditional use in pain treatment. Furthermore, GLcN and taurine contribute, at least in part, to the anti-inflammatory activity of AV.


Assuntos
Analgésicos/farmacologia , Anti-Inflamatórios/farmacologia , Isópodes/química , Extratos Vegetais/farmacologia , Animais , Edema/tratamento farmacológico , Feminino , Inflamação/tratamento farmacológico , Masculino , Camundongos , Dor/tratamento farmacológico , Medição da Dor , Fitoterapia , Extratos Vegetais/química , Água/química
6.
Molecules ; 22(2)2017 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-28208826

RESUMO

Cancer is one of the most common lethal diseases, and natural products have been extensively studied as anticancer agents considering their availability, low toxicity, and economic affordability. Plants belonging to the genus Aconitum have been widely used medically in many Asian countries since ancient times. These plants have been proven effective for treating several types of cancer, such as lung, stomach, and liver cancers. The main effective components of Aconitum plants are diterpenoid alkaloids-which are divided into C18-, C19-, C20-, and bis-diterpenoid alkaloids-are reportedly some of the most promising, naturally abundant compounds for treating cancer. This review focuses on the progress of diterpenoid alkaloids with different structures derived from Aconitum plants and some of their derivatives with potential anticancer activities. We hope that this work can serve as a reference for further developing Aconitum diterpenoid alkaloids as anticancer agents.


Assuntos
Aconitum/química , Alcaloides/química , Alcaloides/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Diterpenos/química , Animais , Proliferação de Células/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Humanos , Estrutura Molecular
7.
Chin J Integr Med ; 22(6): 445-50, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25399307

RESUMO

OBJECTIVE: Mahuang-Shigao herb-pair is a famous formula composed of Ephedra and Gypsum. The herb-pair is frequently used for treating cold symptoms and bronchial asthma in the clinical practice of Chinese medicine (CM). In the present study, we evaluated evidence for the benefit of combined use of Ephedra and Gypsum by analyzing the antipyretic and anti-asthmatic activities of Ephedra-Gypsum. METHODS: The antipyretic effects of Ephedra-Gypsum were evaluated in yeast-induced hyperthermia test. Thirty male Wistar rats were randomly divided into 5 groups, including control group, standard aspirin group, and 3 Ephedra- Gypsum groups of different doses (6, 12, 24 g/kg). Ephedra-Gypsum extract and asprin were administered orally 6 h after the injection of yeast solution and body temperature was measured every 1 h for 8 h. The antiasthmatic effects of Ephedra-Gypsum were evaluated using an ovalbumin (OVA)-induced asthmatic rat model. Thirty-six male SD rats were randomly divided into 6 groups. Rats were alternately sensitized and OVA+Al(OH) challenged by exposure to mists of ovalbumin. Ephedra-Gypsum extracts (6, 12, 24 g/kg) or dexamethasone were administered 45 min prior to the allergen challenge for 8 days. Latent period and the weight of wet to dry ratio of lung were determined. In addition, the eosinophils in blood and white blood cell (WBC) were counted by an YZ-Hemavet Analyzer. RESULTS: The Ephedra-Gypsum extracts at test dose (6, 12, 24 g/kg) significantly and dose-dependently attenuated yeast-induced fever in rats. The Ephedra-Gypsum extracts also prolonged the latent period, reduced OVA-induced increases in eosinophils and WBC, and decreased the wet and dry weight ratio of the lungs in the anti-asthmatic test. CONCLUSIONS: These findings indicate that the Ephedra-Gypsum extract has antipyretic and anti-asthmatic properties. Hence, the results support additional scientific evidence in prescriptions.


Assuntos
Antiasmáticos/uso terapêutico , Antipiréticos/uso terapêutico , Sulfato de Cálcio/uso terapêutico , Medicamentos de Ervas Chinesas/uso terapêutico , Ephedra/química , Extratos Vegetais/uso terapêutico , Alcaloides/análise , Animais , Asma/tratamento farmacológico , Febre/tratamento farmacológico , Pulmão/efeitos dos fármacos , Pulmão/patologia , Masculino , Tamanho do Órgão/efeitos dos fármacos , Ovalbumina , Ratos Sprague-Dawley , Ratos Wistar
8.
Zhongguo Zhong Yao Za Zhi ; 40(5): 963-70, 2015 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-26087564

RESUMO

In this study, UPLC-MS/MS was adopted to determine the contents of five ephedrine alkaloids (Norephedrine, Norpseudoephedrine, Ephedrine, Pseudoephedrine, Methylephedrine) in plasma and urine in rats after the combined administration of Ephedrae Herba-Gypsum Fibrosum and calculate relevant pharmacokinetic parameters, in order to discuss the effect of the combined administration of Ephedrae Herba-Gypsum Fibrosum on plasma pharmacokinetics and urinary excretion characteristics. According to the results, after being combined with Gypsum, the five ephedrine alkaloids showed similar pharmacokinetic changes, such as shortened t(max), accelerated absorption rate, but reduced AUC(0-t) and V(z)/F, which may be related to the increase in urine excretion. Besides, Gypsum was added to enhance C(max) of Pseudoephedrine and prolong MRT(0-t) of Methylephedrine, so as to enhance the anti-asthmatic effect of Ephedrae Herba and resist the toxic effect of Norephedrine and Ephedrine. This study proved the scientific compatibility of Ephedrae Herba-Gypsum Fibrosum and provided a reference for studies on the prescription compatibility regularity and relevant practices.


Assuntos
Alcaloides/farmacocinética , Sulfato de Cálcio/farmacocinética , Medicamentos de Ervas Chinesas/farmacocinética , Ephedra/química , Alcaloides/sangue , Alcaloides/urina , Animais , Masculino , Ratos , Ratos Sprague-Dawley , Urina/química
9.
Artigo em Inglês | MEDLINE | ID: mdl-25691910

RESUMO

Herb Ephedra (Ma Huang in Chinese) and Ramulus Cinnamomi (Gui Zhi in Chinese) are traditional Chinese herbs, often used together to treat asthma, nose and lung congestion, and fever with anhidrosis. Due to the adverse effects of ephedrine, clinical use of Ma Huang is restricted. However, Gui Zhi extract has been reported to decrease spontaneous activity in rats and exert anti-inflammatory and neuroprotective effects. The present study explored the possible inhibitory effect of Gui Zhi on Ma Huang-induced neurotoxicity in rats when the two herbs were used in combination. All Ma Huang and Ma Huang-Gui Zhi herb pair extracts were prepared using methods of traditional Chinese medicine and were normalized based on the ephedrine content. Two-month-old male Sprague-Dawley rats (n = 6 rats/group) were administered Ma Huang or the Ma Huang-Gui Zhi herb pair extracts for 7 days (ephedrine = 48 mg/kg), and locomotor activity was measured. After 7 days, oxidative damage in the prefrontal cortex was measured. Gui Zhi decreased hyperactivity and sensitization produced by repeated Ma Huang administration and attenuated oxidative stress induced by Ma Huang. The results of this study demonstrate the neuroprotective potential of Gui Zhi in Ma Huang-induced hyperactivity and oxidative damage in the prefrontal cortex of rats when used in combination.

10.
J Ethnopharmacol ; 155(1): 642-8, 2014 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-24929107

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Herba Ephedra (Mahuang in Chinese), is derived from dried Ephedra sinica Stapf stems and has been widely used to treat the common cold, coughs, asthma, and edema for thousands of years. The Mahuang-Guizhi herb-pair is a famous formula composed of Mahuang and Ramulus Cinnamomi (Guizhi in Chinese, the dried twig of Cinnamomum cassia Presl.), used to improve pharmacological effects and reduce toxicity. In order to investigate the influence of Mahuang-Guizhi herb-pair ratios on bioavailability, the plasma pharmacokinetics profiles of five ephedrine alkaloids were compared following oral administration of four different ratios to rats. MATERIALS AND METHODS: Sprague-Dawley rats were randomly assigned to four groups and orally administered Mahuang-Guizhi (ratios 3:0; 3:1; 3:2; 3:4, w/w). Assays for five ephedrine alkaloids (ephedrine, pseudoephedrine, methylephedrine, norephedrine, and norpseudoephedrine) were developed and validated using ultra-high-performance liquid chromatography tandem mass spectrometry coupled with liquid-liquid extraction. RESULTS: Key pharmacokinetic parameters of the five ephedrine alkaloids (maximal plasma concentration, mean residence time, and half-life) were significantly different (p<0.05) after oral administration of Mahuang-Guizhi herb-pair ratios, as compared to those of Mahuang. CONCLUSION: Ephedrine alkaloid pharmacokinetic differences in rat plasma could help explain previous findings of pharmacological and toxicity differences between Mahuang and Mahuang-Guizhi herb-pair preparations. These results could facilitate future studies to increase the efficacy and decrease the toxicity of Mahuang and Guizhi.


Assuntos
Alcaloides/farmacocinética , Cinnamomum aromaticum/química , Ephedra sinica/química , Administração Oral , Alcaloides/administração & dosagem , Alcaloides/isolamento & purificação , Animais , Disponibilidade Biológica , Cromatografia Líquida de Alta Pressão/métodos , Meia-Vida , Extração Líquido-Líquido , Ratos , Ratos Sprague-Dawley , Espectrometria de Massas em Tandem/métodos
11.
Zhong Yao Cai ; 37(12): 2234-9, 2014 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-26080511

RESUMO

OBJECTIVE: Five kinds of Ephedra alkaloids (NME, NMP, E, PE and ME) in Ephedrae Herba extracts and Ephedrae Herba-Atractylodis Macrocephalae Rhizoma herbal pair extracts of plasma pharmacokinetic and tissue distribution study in rats were carried out, to discuss the changes of Ephedrae Herba compatibility with Atractylodis Macrocephalae Rhizoma before and after METHODS: HPLC- MS method was used and the condition was as flollows: ZORBAX SB-C18 column (100 mm x 2.1 mm, 3.5 µm), column temperature of 35 °C, mobile phase of ACE-0.1% formic solution in gradient elution mode, flow rate at 0.4 mL/min; MRM positive ion detection mode. RESULTS: The distribution trends of Ephedra alkaloids were changing in plasma and tissues of rats after Ephedrae Herba compati- bility with Atractylodis Macrocephalae Rhizoma. CONCLUSION: Ephedrae Herba compatibility with Atractylodis Macrocephalae Rhizoma may increase drug efficacy and reduce the toxicity of Ephedra alkaloids at the same time.


Assuntos
Alcaloides/farmacocinética , Medicamentos de Ervas Chinesas/farmacocinética , Animais , Atractylodes/química , Cromatografia Líquida de Alta Pressão , Ephedra/química , Espectrometria de Massas , Ratos , Rizoma/química , Distribuição Tecidual
12.
Zhong Yao Cai ; 34(6): 896-901, 2011 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-22017005

RESUMO

OBJECTIVE: To evaluate the correlativity between volatile oils of Mahuang Fuzi Xixin decoction and its major constituted herbs in composition and proportion. METHODS: The chemical compositions of volatile oils obtained by wet distillation from Mahuang Fuzi Xixin decoction and its major constituted herbs (Herba Ephedrae, processed Radix Aconiti Lateralis, Herba Asari) were analyzed by GC-MS. RESULTS: 44 volatile components of Mahuang Fuzi Xixin decoction were identified, which mainly derived from its constituted herbs Mahuang and Xixin. 68, 8, 39 volatile components were respectively identified from volatile oils of Herba Epherae, processd Radix Aconiti Lateralis and Herba Asari. There were apparent changes in composition and proportion between volatile oils of Mahuang Fuzi Xixin decoction and its major constituted herbs. CONCLUSION: There are apparent changes not only in quantity but also in quality between decoction and its major constituted herbs. Meanwhile any changes in composition may lead to a change in efficacy.


Assuntos
Aconitum/química , Asarum/química , Medicamentos de Ervas Chinesas/química , Ephedra sinica/química , Óleos Voláteis/análise , Medicamentos de Ervas Chinesas/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Óleos Voláteis/química , Plantas Medicinais/química
13.
Zhong Yao Cai ; 34(4): 620-3, 2011 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-21809550

RESUMO

OBJECTIVE: To establish the method for determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride in maxingshigan decoction by capillary electrophoresis. METHODS: The separation was performed on a fused silica capillary of 60 cm x 55 microcrpm ID (52 cm of effective length). 60 mmol/L NaB4O7 + 10% (V/V) CH3OH (pH 9.0) was selected as the running buffer. The separation voltage was 12 kV. The samples was injected by gravity (10 s, 15 cm). The detection wavelength was 210 nm and berberine hydrochloride was the internal standard. RESULTS: The linear range of determination for ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride were 20.0-160.0 microg/mL (r = 0.9999), 7.5-60.0 microg/mL (r = 0.9991) and 2.0-10.0 microg/mL (r = 0.9993). The average recoveries were 98.0%, 97.0% and 97.8%, the precisions of the method were 2.31%, 2.21% and 2.00% (n=6), respectively. CONCLUSION: The method is convenient, rapid and accurate for the quality control of maxingshigan decoction.


Assuntos
Medicamentos de Ervas Chinesas/química , Eletroforese Capilar/métodos , Efedrina/análise , Plantas Medicinais/química , Pseudoefedrina/análise , Medicamentos de Ervas Chinesas/análise , Ephedra sinica/química , Efedrina/análogos & derivados , Controle de Qualidade , Reprodutibilidade dos Testes
14.
Zhong Yao Cai ; 33(2): 236-9, 2010 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-20575419

RESUMO

OBJECTIVE: To discuss the significance of processing and complex prescription with Herba Ephedrae, the effects of Herba Ephedrae, Honey-fried Herba Ephedrae and Maxingshigan decoction on autonomic activities in mice were compared. METHODS: 110 female Kunming mice were divided into 11 groups, namely normal saline group (NS), ephedrine group (E), high dose Herba Ephedrae group (MH-H), moderate dose Herba Ephedrae group (MH-M), low dose Herba Ephedrae group (MH-L) ,high dose Honey-fried Herba Ephedrae group (ZMH-H), moderate dose Honey-fried Herba Ephedrae group (ZMH-M),low dose Honey-fried Herba Ephedrae group (ZMH-L), high dose Maxingshigan decoction group (MX-H), moderate dose Maxingshigan decoction group (MX-M) and low dose Maxingshigan decoction group (MX-L). The numbers of autonomic activity in 15 minutes before intragastric administration (ig), and after ig 0.5, 1, 2, 3, 4 h were determined. RESULTS: There was interaction between time and groups. There were very significant differences between before ig and all time-points after ig (P < 0.01). 30 minutes after ig,there were significant differences between E and NS,E and ZMH-L,E and MX-L (P < 0.05). 1 h after ig, there were significant differences between MX-M and NS (P < 0.05). 3 h after ig, there were significant differences between MX-M and MH-L (P < 0.05). 30 minutes after ig, both ZMH-L and MX-L could reduce the number of autonomic activity in some extent compare with MH-L. 1 h or 2 h after ig, ZMH-L could reduce the number of autonomic activity in some extent compare with MH-L 4 h after ig, MX-L could reduce the number of autonomic activity in some extent compare with MH-L. CONCLUSION: Under the condition of this study, regarding autonomic activity as index, both ZMH-L and MX-L can reduce center stimulation in some extent.


Assuntos
Sistema Nervoso Central/efeitos dos fármacos , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Ephedra sinica/química , Atividade Motora/efeitos dos fármacos , Animais , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/química , Efedrina/administração & dosagem , Efedrina/farmacologia , Feminino , Camundongos , Camundongos Endogâmicos , Plantas Medicinais/química , Distribuição Aleatória , Tecnologia Farmacêutica/métodos , Fatores de Tempo
15.
Nan Fang Yi Ke Da Xue Xue Bao ; 30(1): 121-2, 2010 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-20118000

RESUMO

OBJECTIVE: To observe the effects of Herba ephedrae, honey-fried Herba ephedrae and Maxingshigan decoction on pentobabital sodium sleep experiment in mice. METHODS: Male Kunming mice were divided into 11 groups, namely normal saline (NS) group, ephedrine group, 3 Herba ephedrae dose (high, medium, and low) groups, 3 honey-fried Herba ephedrae dose group, and 3 Maxingshigan decoction dose groups. The corresponding drugs were administered intragastrically for 6 consecutive days, and 45 min after the final administration, the mice received intraperitoneal injection of pentobabital sodium, and the latent period and continuous sleeping time were recorded. RESULTS: Compared with high- and low-dose Herba ephedrae groups, Maxinshigan decoction containing equivalent Herba ephedrae significantly increased the sleeping time of the mice (P<0.05). In comparison with NS, the decoction at medium and low doses produced no significant changes in the sleeping time, which, however, was significantly shortened in the other 8 groups (P<0.05). Compared with Herba ephedrae, Maxingshigan decoction and honey-fried Herba ephedrae at equivalent doses showed comparable effects on the sleep latency (P>0.05). CONCLUSION: Under the condition of this experiment and with pentobabital sodium-induced sleeping time as the index, honey-fried Herba ephedrae shows no obvious effect in reducing the excitement of the central nervous system, while Maxingshigan decoction can significantly lower the excitement level, the effect of which is inversely correlated to the dose administered.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Ephedra sinica/química , Pentobarbital/farmacologia , Sono/efeitos dos fármacos , Animais , Sistema Nervoso Central/efeitos dos fármacos , Relação Dose-Resposta a Droga , Masculino , Camundongos , Distribuição Aleatória
16.
Zhong Yao Cai ; 33(11): 1791-4, 2010 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-21434444

RESUMO

OBJECTIVE: To investigate the multiple bioactive constituents in Gegen Qinlian decoction and evaluate its bioactivity and quality control. METHODS: A high performance liquid chromatography with diode array detection (HPLC-DAD) method for the simultaneous qualitative determination of multiple components was developed. The separation was performed on a Kromasil C18 column by gradient elution with acetonitrile and ammonium acetate (containing 0.3% triethylamine, and adjusted pH 4. 3 using 1% glacial acetic acid) as the mobile phase at a flow-rate of 0.7 mL/min, the column temperature was maintained at 30 degrees C, HPLC chromatographic comparison between Gegen Qinlian decoction, four medicinal material and 14 components were carried out to investigate components in the composite formula. RESULTS: 23 characteristic peaks were identified and could be dated back to four medicinal materials from Gegen Qinlian decoction, in which 12 peaks were identifed by standards. CONCLUSION: This readily available, low-cost and reliable HPLC-DAD method can be used for simultaneous analysis of multiple bioactive constituents of Gegen Qinlian decoction and will improve the quality control of Gegen Qinlian decoction.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Flavonas/análise , Plantas Medicinais/química , Alcaloides/análise , Combinação de Medicamentos , Medicamentos de Ervas Chinesas/isolamento & purificação , Raízes de Plantas/química , Controle de Qualidade , Reprodutibilidade dos Testes , Saponinas/análise
17.
Yao Xue Xue Bao ; 44(12): 1391-6, 2009 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-21351475

RESUMO

This paper is to report the analysis of the main chemical constituents of Shuanghuanglian injection powder and determination of their origin. The sample solution was analyzed by a Zorbax C18 column with a gradient mobile phase comprised of methanol and 0.25% acetic acid solution. Both UV and electrospray ionization mass spectrometry detector were used simultaneously, -Q1-scan detection mode was evaluated for the identification of the LC peaks. To analyze the mass spectrum of every LC peaks, 43 molecular mass from the ion chromatogram of Shuanghuanglian injection powder were identified and among them, structure of 20 compounds were elucidated, and the data were sorted to the three component herbs, separately.


Assuntos
Ácido Clorogênico/análise , Medicamentos de Ervas Chinesas/química , Flavonoides/análise , Glicosídeos/análise , Plantas Medicinais/química , Cromatografia Líquida de Alta Pressão/métodos , Combinação de Medicamentos , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/isolamento & purificação , Forsythia/química , Injeções , Lonicera/química , Pós , Scutellaria/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem/métodos
18.
Zhong Yao Cai ; 31(4): 522-4, 2008 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-18661823

RESUMO

OBJECTIVE: To study the chemical constituents of Hedyotis diffusa. METHODS: Column chromatographic techniques were used for isolation and purification of chemical constituents of this plant and their structures were identified by spectroscopic analysis. RESULTS: Five compounds were isolated and identified as scandoside methyl ester (I), deacetylasperulosidic acid methyl ester (II), 10-dehydrogeniposide (III), 2-hydroxy-3-methylanthraquinone (IV), stigmasterol (V), respectively. CONCLUSION: Compound II as iridoids was isolated from Hedyotis diffusa for the first time, compound III as iridoids was isolated from genus Hedyotis for the first time.


Assuntos
Antraquinonas/isolamento & purificação , Ácidos Cumáricos/isolamento & purificação , Hedyotis/química , Iridoides/isolamento & purificação , Plantas Medicinais/química , Antraquinonas/química , Ácidos Cumáricos/química , Iridoides/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Estigmasterol/química , Estigmasterol/isolamento & purificação
19.
Nan Fang Yi Ke Da Xue Xue Bao ; 28(1): 52-3, 2008 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-18227026

RESUMO

OBJECTIVE: To prepare sustained-release capsules of cinnamon oil with stearic acid, polyethylene glycol 6000 and gluceryl monostearate. METHODS: After the solid dispersion of cinnamon oil was prepared by melting method, the effects of the process variables and formulation variables on solid dispersion and dissolution were investigated. The formulation and preparation process of cinnamon oil solid dispersion were optimized by orthogonal design, and Fourier-transformed infrared (FTIR) spectroscopy was employed to detect the possible molecular interaction between cinnamon oil and the adjuvants. RESULTS: The in vitro release percentage of the optimized formula complied with the Higuchi equation, and the preparation allowed drug delivery for 12 h. Analysis with FTIR spectroscopy revealed interactions between cinnamon oil and the adjuvents and formation of the solid dispersion. CONCLUSION: The solid dispersion capsules prepared through this simple and well reproducible process allow obviously sustained release of cinnamon oil.


Assuntos
Cinnamomum zeylanicum/química , Preparações de Ação Retardada/química , Medicamentos de Ervas Chinesas/química , Óleos Voláteis/química , Cápsulas , Preparações de Ação Retardada/farmacocinética , Composição de Medicamentos , Medicamentos de Ervas Chinesas/farmacocinética , Óleos Voláteis/farmacocinética , Tecnologia Farmacêutica
20.
Nan Fang Yi Ke Da Xue Xue Bao ; 28(1): 127-8, 2008 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-18227043

RESUMO

OBJECTIVE: To analyze the chemical constituents of Hedyotis diffusa. METHODS: Column chromatographies were used to isolate and purify the chemical constituents of this plant, and their structures were identified by spectral analysis and physicochemical properties. RESULTS AND CONCLUSION: Seven compounds were isolated and identified as p-coumaric acid (I), methyl-p-coumarate (II), 2-formyl-5- hydroxymethylfuran (III), quercetin (IV), kaempferol (V), beta-sitosterol(VI) and daucosterol(VII), respectively, among which the compounds II and III were isolated from Hedyotis diffusa for the first time.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Hedyotis/química , Cinamatos/isolamento & purificação , Ácidos Cumáricos/isolamento & purificação , Furanos/isolamento & purificação , Quempferóis/isolamento & purificação , Propionatos , Quercetina/isolamento & purificação
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