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1.
Chemosphere ; 251: 126406, 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-32151805

RESUMO

Immobilized microalgae have great potential on the nutrient removal during wastewater treatment. However, their applications are challenged by how to cost-effectively maintain and preserve large number of viable and active microalgal cells. In this study, the cells of Chlorella sacchrarophila were immobilized in the agar containing with algal nutrient, encapsulated in a transparent package and preserved at room temperature. After the preservation for 200 days, microalgal cells with viability of 47-52% were maintained and could be quickly revived after the cultivation in fresh algal medium. Based on the agar-immobilized microalgae, the NH4+-N and PO43--P were efficiently removed from batch and continuous cultures, with the highest removal efficiencies ranging from 96% to 99% were observed. Even being recycled for eight times, the agar-immobilized microalgae were still able to remove 94% of NH4+-N and 66% of PO43--P. Moreover, more than 60% of the nutrient removal efficiency was determined even the agar-immobilized microalgae being preserved for 120 days at room temperature. This work provides a simple, cost-effective and practicable method for the long-term preservation of microalgae at room temperature, which makes the application of microalgal species on the nutrient removal during wastewater treatment more convenient and useful.

2.
Bioresour Technol ; 302: 122879, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32028148

RESUMO

A cost-effective and environmentally friendly method for biofuel production was developed, by utilizing duckweed as feedstock for biohydrogen production through dark fermentation and simultaneously using the fermentative waste to produce microalgal lipids. The results suggested that acid hydrolysis (1% H2SO4) was more suitable for the pretreatment of duckweed biomass. Maximum hydrogen production of 169.30 mL g-1 dry weight was determined under a temperature of 35 °C and an initial pH of 7.0. After the dark fermentation, the volatile fatty acids (VFAs) including acetate and butyrate, were detected in the waste, with concentration determined as 1.04 g L-1 and 1.52 g L-1, respectively. During the mixotrophic cultivation of Chlorella sacchrarophila FACHB-4 using waste as feedstock, the maximum microalgal biomass and the lipid productions were about 2.8 and 33 times higher with respect to the autotrophic growth. The simultaneous biohydrogen production and waste utilization method provided a green strategy for biofuel production.


Assuntos
Chlorella , Microalgas , Biocombustíveis , Biomassa , Fermentação , Hidrogênio , Lipídeos
3.
FEBS Open Bio ; 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31984680

RESUMO

Colorectal cancer (CRC) is the third main cause of cancer-relevant deaths worldwide, and its incidence has increased in recent decades. Previous studies have indicated that certain long non-coding RNAs (lncRNAs) have regulatory roles in tumor occurrence and progression. Often, lncRNAs are competitive endogenous RNAs (ceRNAs) which sponge miRNAs to up-regulate mRNAs. Here, we examined the role of a novel lncRNA BBOX1 antisense RNA 1 (BBOX1-AS1) in colorectal cancer (CRC). We observed that BBOX1-AS1 is overexpressed in CRC cell lines, and BBOX1-AS1 knockdown enhances cell proliferation, migration and invasion while reducing cell apoptosis. MiR-361-3p is present at a low level in CRC and is negatively modified by BBOX1-AS1. Moreover, miR-361-3p was validated to be targeted by BBOX1-AS1. SH2B1 was notably up-regulated in CRC cell lines, and identified as a downstream gene of miR-361-3p. In addition, we found that miR-361-3p amplification can suppress the expression of SH2B1. Finally, data from rescue assays suggested that overexpression of SH2B1 counteracted BBOX1-AS1 silencing-mediated inhibition of CRC progression. In conclusion, BBOX1-AS1 promotes CRC progression by sponging hsa-miR-361-3p and up-regulating SH2B1.

4.
Front Microbiol ; 10: 875, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31105671

RESUMO

Ammonia-oxidizing archaea (AOA) are widely distributed on the earth and play a significant role in the global nitrogen cycle. Although dozens of AOA strains were obtained in the last 13 years, it is still necessary to obtain more AOA strains for the entire exploration of their ecology, physiology, and underlying biochemistry in different environments. In this study, we designed a two-step strategy for the rapid enrichment of Nitrosocosmicus-like AOA from soils. Firstly, combination of kanamycin and ampicillin was chosen as the selective stress for bacteria and quartz sands were used as the attachment of AOA cells during the first step cultivation; only after 40-75 days cultivation, AOA enrichments with abundance >20% were obtained. Secondly, combination of ciprofloxacin and azithromycin was chosen as the selective stress for the following cultivation; it is able to penetrate the biofilms and kill the bacterial cells inside the aggregate, contributing to the AOA enrichments reached high abundances (90%) only after one-time cultivation. Basing on this strategy, three AOA strains were obtained from agricultural soils only after 90-150 days cultivation. Phylogenetic analysis suggested these AOA belong to the soil group I.1b Thaumarchaeota and are closely related to the genus Nitrosocosmicus. In general, AOA enrichment or isolation is very difficult and time-consuming (an average of 2-3 years). Here, we provide a new strategy for the rapid enrichment of high abundance of Nitrosocosmicus-like AOA from soil, which gives a new solution to the AOA enrichment and cultivation in a short period.

5.
Ecotoxicol Environ Saf ; 172: 451-459, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30735978

RESUMO

Antibiotic resistance genes (ARGs) that distributed in antibiotic resistant bacteria (ARBs) are widespread in aquaculture and have great threats to the aquatic organism as well as to human. However, our understanding about the risk of ARGs to the health of aquatic organism is still limited. In the present study, we got a deep insight into the diversity of ARGs in the intestinal bacteria of shrimp by culture-dependent and independent approaches. Results of the PCR-based detection and culture-dependent analysis indicated that the tetracycline, sulfadiazine, quinolone and erythromycin resistance genes were prevalent in the commercial shrimps that bought from aquatic markets or supermarket. The culture-independent plasmid metagenomic analysis identified 62 different ARGs, which were classified into 21 types, with abundances ranging from 13 to 1418 ppm. The analysis suggested that most of the ARGs come from the plasmids originating from Vibrio (accounted for 2.8-51%) and Aeromonas (accounted for 16-55%), and the Vibrio group was concluded to be the main bacterial pathogen that probably resulted in the shrimp disease. Accordingly, the plasmid metagenomic that focuses on the mobile genetic elements has great potential on the identification of ARGs in complex environments.


Assuntos
Antibacterianos/farmacologia , DNA Bacteriano/isolamento & purificação , Farmacorresistência Bacteriana Múltipla/genética , Penaeidae/microbiologia , Animais , Aquicultura , Contagem de Colônia Microbiana , DNA Bacteriano/genética , Trato Gastrointestinal/microbiologia , Genes Bacterianos , Metagenômica , Plasmídeos/efeitos dos fármacos , Vibrio/efeitos dos fármacos , Vibrio/genética , Vibrio/metabolismo
6.
Bioresour Technol ; 275: 425-429, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30594343

RESUMO

Reduction of chlorophyll size has great advantages on improving the photosynthesis efficiency as well as the photolysis algal H2 production. To promote the H2 production, Chlamydomonas reinhardtii was mutated by atmospheric and room temperature plasma (ARTP). After the selection, an algal mutant was observed to have 1.8-5.2 times (28.5-84.1 mL L-1) and 2.7-3.1 times (356.5-405.2 mL L-1) higher H2 production than wild-type during the algal subcultures grown in pure and co-cultures, respectively. In comparison with wild-type alga, the mutant grew as lighter green colonies on agar plate, with about 2 times larger cell diameter and 5.3-6.1 times lower chlorophyll content per unit cell volume. Results from the comparative transcriptomic analysis indicated that most of the genes relating to photosynthesis (photosystem I, II, cytochrome b6/f complex, photosynthetic electron transport, and F-type ATPase) and LHC proteins were have higher expressions in mutant cells, suggesting the improvement of photosynthesis efficiency.


Assuntos
Chlamydomonas reinhardtii/metabolismo , Clorofila/metabolismo , Hidrogênio/metabolismo , Fotossíntese , Temperatura Ambiente
7.
Bioresour Technol ; 266: 109-115, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29958148

RESUMO

In this study, a saline-alkaline tolerant microalgal strain was isolated and identified as Chlorella sp. LPF. This strain was able to grow at pH values up to 10 and at salinities up to 5%, and tolerated to 80 g L-1 of sodium bicarbonate. The utilization of bicarbonate as carbon source significantly promoted microalgal growth and lipid production. In the non-sterile cultivation supplying with 80 g L-1 of sodium bicarbonate, the microalgal growth had no difference with their growth in the sterile medium; however, the bacterial growth was suppressed and the cell number decreased to low levels after six days cultivation. This study gives an insight into the potential that using high concentration of sodium bicarbonate as external carbon source to cultivate microalga in non-sterile condition, and suggests a possibility of using bicarbonate as growth promoter and antibacterial agent for the microalgal outdoor cultivation.


Assuntos
Carbono/metabolismo , Microalgas , Bicarbonato de Sódio , Biomassa , Chlorella , Lipídeos
8.
Am J Transl Res ; 10(2): 639-647, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29511458

RESUMO

Inconsistent results of Sex-determining region Y-box2 (SOX2) expression have been reported in gastric cancer (GC) before. Our recent studies showed that SOX2 was significantly downregulated in GC cells compared with GES-1 at both mRNA and protein level. Transfected with pcDNA3.1-SOX2 resulted in enforced expression of SOX2 at mRNA and protein levels compared with NC group in undifferentiated cell lines including HGC27 and BGC823. MTT assay showed that exogenous expressed SOX2 suppressed cell proliferation. FC analysis revealed that SOX2-overexpressing cells exhibited cell-cycle arrest and apoptosis. Transwell assay showed the anti-metastatic effect of SOX2 in GC cells. The subsequent results suggested CCND1 and PARP were downregulated in SOX2 overexpressed GC cells, and were responsible for the SOX2-induced anticancer effects. Thus, SOX2 proved to be an expected biomarker in GC diagnosis.

9.
Bioresour Technol ; 251: 350-357, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29291532

RESUMO

In this study, bacterium Pseudomonas sp. strain D was proved to be the main partner assisting Chlamydomonas reinhardtii in improving photolysis-mediated H2 production and a good partner for promoting H2 production by the green algae Chlorella and Scenedesmus. In strain D partnered algal-bacterial co-culture, the relative O2 content in the headspace plus the dissolved oxygen in the culture medium were rapidly consumed by bacterial growth, resulting in a completely anaerobic environment that proved suitable for the activation of algal hydrogenase. Moreover, algal-bacterial cooperation was able to slow the reduction of chlorophyll, enhance starch accumulation, and maintain protein content, which are the potential factors whose control provides an opportunity for improving algal H2 production. This study systematically analyzed the main pathway responsible for H2 production by algal-bacterial cooperation and the potential mechanisms for improvement, and proposed an efficient and durable algal-bacterial cooperation system for improving photolysis-mediated H2 production by green algae.


Assuntos
Hidrogênio , Fotólise , Chlamydomonas reinhardtii , Chlorella , Hidrogenase
10.
3 Biotech ; 8(1): 73, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29354384

RESUMO

In this study, we surveyed the abundance and diversity of three sulfur oxidation genes (sqr, soxB, and dsrA) using quantitative assays and Miseq high-throughput sequencing. The quantitative assays revealed that soxB is more abundant than sqr and dsrA and is the main contributor to sulfur oxidation. In the diversity analysis, the SOB community mainly comprised the classes Nitrospira, Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. The genera Gallionella, Hydrogenophaga, Limnohabitans, Methylomonas, Nitrospira, Rhodoferax, and Sulfuritalea were abundant in the communities for sqr; Dechloromonas, Limnohabitans, Paracoccus, Sulfuritalea, Sulfitobacter, and Thiobacillus were abundant in communities for soxB; Sulfuritalea, Sulfurisoma, and Thiobacillus were abundant in communities for dsrA. This study presented a high diversity of SOB species and functional sulfur-oxidizing genes in Pearl River via high-throughput sequencing, suggesting that the aquatic ecosystem has great potential to scavenge the sulfur pollutants by itself.

11.
Biomed Res Int ; 2017: 6304248, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28798934

RESUMO

Cellvibrio sp. PR1 is a xylanolytic and agarolytic bacterium isolated from the Pearl River. Strain PR1 is closely related to Cellvibrio fibrivorans and C. ostraviensis (identity > 98%). The xylanase and agarase contents of strain PR1 reach up to 15.4 and 25.9 U/mL, respectively. The major cellular fatty acids consisted of C16:0 (36.7%), C18:0 (8.8%), C20:0 (6.8%), C15:0 iso 2-OH or/and C16:1ω7c (17.4%), and C18:1ω7c or/and C18:1ω6c (6.7%). A total of 251 CAZyme modules (63 CBMs, 20 CEs, 128 GHs, 38 GTs, and 2 PLs) were identified from 3,730 predicted proteins. Genomic analysis suggested that strain PR1 has a complete xylan-hydrolyzing (5 ß-xylanases, 16 ß-xylosidases, 17 α-arabinofuranosidases, 9 acetyl xylan esterases, 4 α-glucuronidases, and 2 ferulic acid esterases) and agar-hydrolyzing enzyme system (2 ß-agarases and 2 α-neoagarooligosaccharide hydrolases). In addition, the main metabolic pathways of xylose, arabinose, and galactose are established in the genome-wide analysis. This study shows that strain PR1 contains a large number of glycoside hydrolases.


Assuntos
Proteínas de Bactérias/genética , Cellvibrio/genética , Genoma Bacteriano , Glicosídeo Hidrolases/genética , Rios/microbiologia , Microbiologia da Água , Proteínas de Bactérias/biossíntese , Cellvibrio/enzimologia , Cellvibrio/isolamento & purificação , Glicosídeo Hidrolases/biossíntese
12.
Bioresour Technol ; 227: 404-407, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28057409

RESUMO

In this study, the toxicity of ammonium was removed in the microalga Nannochloropsis oculata by using acetate as a carbon source. Algal biomass and lipid production were significantly enhanced when N. oculata was grew on 0.5-50mM of ammonium and 16-64mM of acetate in mixotrophic conditions. When grown mixotrophically on 1mM of ammonium and 32mM of acetate, the biomass and lipid production reached 543mg/L and 279mg/L respectively, which were 1.5 and 9.4times higher than the levels generated when grown autotrophically on nitrate. This suggests that mixotrophic growth with acetate can be a useful method to enhance microalgal lipid production.


Assuntos
Compostos de Amônio/isolamento & purificação , Biomassa , Lipídeos/biossíntese , Microalgas/química , Microalgas/metabolismo , Compostos de Amônio/análise , Inativação Metabólica , Microalgas/crescimento & desenvolvimento
13.
Am J Transl Res ; 9(12): 5671-5677, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29312519

RESUMO

Signal transducer and activator of transcription-3 (STAT3) is closely associated with tumorigenesis and is activated in tumor cells of a variety of cancers. The raised expression of cyclin D1 (CCND1) by activated STAT3 has been verified in some cancers. However, the relationship between STAT3 and CCND1 has yet to be studied in gastric cancer (GC) cells. In the present study, we found that STAT3 was constitutively activated in several GC cells together with overexpressed CCND1. In addition, IL-6 treatment enhanced the expression level of p-STAT3 and CCND1, accelerating the cell cycle progress and transferring from G1 to S phase. The increased proliferation, migration and invasion were also demonstrated by the treatment of IL-6 in HGC-27 and BGC-823 cells. While AG490 treatment, a Janus Kinase (JAK) inhibitor, showed the opposite effect. Therefore, our research demonstrated the positive correlation between p-STAT3 and CCND1 in GC cells. The constitutive activation of STAT3 and CCND1 overexpression accounted for the proliferation, migration and invasion in GC cells.

14.
Bioresour Technol ; 200: 1050-4, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26508433

RESUMO

In this work, a Cellvibrio pealriver-microalga co-cultivation mode was used to promote the growths of four microalgae by using xylan as feedstock. After 12days of cultivation, the biomass concentrations of Chlorella sacchrarophila, Chlorella pyrenoidosa and Chlamydomonas reinhardtii in co-cultivation were equal to those in mixotrophic growth on glucose, and the Dunaliella was about 1.6-fold higher than that on glucose. The comparative transcriptomes analysis demonstrated that the xylose and xylan hydrolysates were catalyzed to some active substrates by C. pealriver via some functional enzymes; these active substrates are possibly responsible for the promotion of microalgal growth. This C. pealriver-microalga co-cultivation mode is a potential method to produce low-cost microalgal biodiesel by using hemicellulose as feedstock.


Assuntos
Biocombustíveis , Cellvibrio/crescimento & desenvolvimento , Técnicas de Cocultura/métodos , Microalgas/crescimento & desenvolvimento , Xilanos/metabolismo , Biomassa , Cellvibrio/genética , Cellvibrio/metabolismo , Chlamydomonas reinhardtii/crescimento & desenvolvimento , Chlorella/crescimento & desenvolvimento , Perfilação da Expressão Gênica , Glucose/metabolismo , Microalgas/genética , Microalgas/metabolismo
15.
J Biotechnol ; 214: 57-8, 2015 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-26253962

RESUMO

Cellvibrio pealriver PR1 (CGMCC 1.14955=NBRC 110968) was isolated from a freshwater sample from the Pearl River in China. It is able to degrade various carbohydrates such as starch, xylan, agar, cellulose or chitin. The genomic feature and polysaccharide hydrolases of this strain were described in this paper. The total genome size of C. pealriver PR1 is 4,427,922 bp with 3986 coding sequences (CDS), 53 tRNAs, 16 rRNAs and 1 sRNA. The annotated full genome sequence of this strain provides the genetic basis for revealing its role as a xylanolytic and agarolytic bacterium.


Assuntos
Cellvibrio/genética , Água Doce/microbiologia , Genoma Bacteriano/genética , Proteínas de Bactérias , Cellvibrio/enzimologia , DNA Bacteriano/análise , DNA Bacteriano/genética , Endo-1,4-beta-Xilanases , Glicosídeo Hidrolases , Microbiologia da Água
16.
Wei Sheng Wu Xue Bao ; 55(3): 351-7, 2015 Mar 04.
Artigo em Chinês | MEDLINE | ID: mdl-26065277

RESUMO

OBJECTIVE: Nitrite reductase encoded by nirK is a key enzyme to denitrification, and is found in ammonia-oxidizing archaea (AOA). Based on the diversity of nirK, it was good to study the functions of nitrite reductase to AOA on denitrification. METHODS: We constructed nirK gene clone libraries based on the nirK gene PCR products of water, sediment and soil, screened the positive clones by restriction fragment length polymorphism ( RFLP), and sequenced the representative fragments from positive clones. RESULTS: RFLP analysis of the clone libraries shows that there were 10 OTUs in fresh water and sediment, 8 in vegetable soil and its nearby water. Phylogenetic analysis indicated that the amino acid sequences of these nirK were most closely related to the Candidatus Nitrosopumilus koreensis AR1 and Nitrosopumilus maritimus SCM1 with similarities ranging from 53% to 68%. Diversity index of clone libraries shows there were many different types of nirK genes in all samples. Diversity and evenness index of nirK gene of water samples was higher than soil samples whreas vegetable field samples were the richest. CONCLUSION: Thaumarchaeote nirK gene had high diversity in soil and freshwater environments which were very different from ocean sample. The nirK gene encoding nitrite reductase might be important for thaumarchaeote denitrification.


Assuntos
Archaea/enzimologia , Proteínas Arqueais/genética , Bactérias/enzimologia , Água Doce/microbiologia , Variação Genética , Sedimentos Geológicos/microbiologia , Nitrito Redutases/genética , Archaea/classificação , Archaea/genética , Archaea/isolamento & purificação , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Dados de Sequência Molecular , Filogenia , Microbiologia do Solo
17.
Hum Mov Sci ; 40: 163-75, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25574765

RESUMO

In this study, we investigated children's fine motor development by analyzing drawing trajectories, kinematics and kinetics. Straight lines drawing task and circles drawing task were performed by using a force sensitive tablet. Forty right-handed and Chinese mother-tongue students aged 6-12, attending classes from grade 1 to 5, were engaged in the experiment. Three spatial parameters, namely cumulative trace length, vector length of straight line and vertical diameter of circle were determined. Drawing duration, mean drawing velocity, and number of peaks in stroke velocity profile (NPV) were derived as kinematic parameters. Besides mean normal force, two kinetic indices were proposed: normalized force angle regulation (NFR) and variation of fine motor control (VFC) for circles drawing task. The maturation and automation of fine motor ability were reflected by increased drawing velocity, reduced drawing duration, NPV and NFR, with decreased VFC in circles drawing task. Grade and task main effects as well as significant correlations between age and parameters suggest that factors such as schooling, age and task should be considered in the assessment of fine motor skills. Compared with kinematic parameters, findings of NFR and VFC revealed that kinetics is another important perspective in the analysis of fine motor movement.


Assuntos
Destreza Motora/fisiologia , Movimento/fisiologia , Fatores Etários , Fenômenos Biomecânicos , Criança , China , Análise Discriminante , Mãos/fisiologia , Escrita Manual , Humanos
18.
Exp Ther Med ; 9(1): 219-226, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25452806

RESUMO

Previous studies have reported an association between the two coding polymorphisms (91T>A and 169G>A) of the serine/threonine kinase 15 (STK15) gene and the risk of digestive system cancers; however, the results are inconsistent. In the present study, a meta-analysis was carried out to assess the association between the two STK15 polymorphisms and the risk of digestive system cancers. Relevant studies were identified using PubMed, Web of Science, China National Knowledge Infrastructure, WanFang and VIP databases up to February 18, 2014. The pooled odds ratio (OR) with a 95% confidence interval (CI) was calculated using the fixed or random effects model. A total of 15 case-control studies from 14 publications were included. Of these, 15 studies concerned the 91T>A polymorphism and included 7,619 cases and 7,196 controls and four studies concerned the 161G>A polymorphism and included 826 cases and 713 controls. A significantly increased risk of digestive system cancers was observed for the 91T>A polymorphism (recessive model: OR, 1.19; 95% CI, 1.07-1.31). In subgroup analysis by ethnicity, a significant association was detected in Asian populations (recessive model: OR, 1.21; 95% CI, 1.08-1.36) but not in Caucasian and mixed populations. Stratification by tumor type indicated that the 91T>A polymorphism was associated with an increased risk of esophageal and colorectal cancers under the recessive model (OR, 1.19; 95% CI, 1.03-1.38; and OR, 1.24; 95% CI, 1.04-1.46; respectively); however, no significant association was observed between the 169G>A polymorphism and the risk of digestive system cancers in any of the genetic models. Furthermore, in subgroup analysis by ethnicity, similar results were observed in the Asian and Caucasian populations. The present meta-analysis demonstrated that the STK15 gene 91T>A polymorphism, but not the 169G>A polymorphism, may be a risk factor for digestive system cancers, particularly for esophageal and colorectal cancers.

19.
Food Funct ; 5(9): 2338-47, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25088041

RESUMO

Fermented onion products are popular in many countries. We conducted fermentation with and without salt to identify the microorganisms responsible for onion fermentation and the unique taste of fermented onion. The results of PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) revealed that lactic acid bacteria (Lactobacillus zymae, L. malefermentans, L. plantarum), acetic acid bacteria (Acetobacter pasteurianus, A. orientalis), citric acid bacteria (Citrobacter sp., C. freundii), and yeasts (Candida humilis, Kazachstania exigua, Saccharomyces boulardii) were the dominant microorganisms involved in onion fermentation. Organic acid analysis indicated that lactic acid and acetic acid significantly increased after fermentation. There were no significant changes in the types of amino acids after fermentation, but the total concentration of amino acids significantly decreased after fermentation with salt. The increase in esters, alcohols, and aldehydes after fermentation was responsible for the unique flavor of fermented onion. Fermentation with salt inhibited the accumulation of organic acids and limited the conversion of proteins into amino acids but maintained the unique odor of onion by limiting the degradation of sulfur-containing compounds.


Assuntos
Bactérias/metabolismo , Biodiversidade , Aromatizantes/metabolismo , Cebolas/microbiologia , Leveduras/metabolismo , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Fermentação , Dados de Sequência Molecular , Filogenia , Leveduras/classificação , Leveduras/genética , Leveduras/isolamento & purificação
20.
Int J Clin Exp Med ; 7(11): 4174-82, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25550928

RESUMO

Several studies have reported the association between the Asp1104His polymorphism in xeroderma pigmentosum group G (XPG) gene and risk of gastrointestinal cancers. However, the results are inconsistent. This meta-analysis was performed to assess the association between XPG Asp1104His polymorphism and gastrointestinal cancers risk. Relevant studies were identified using PubMed, Web of Science, CNKI, WanFang and VIP databases up to July 22, 2014. The pooled odds ratio (OR) with a 95% confidence interval (CI) was calculated using the fixed- or random effects model. 13 case-control studies from twelve publications with 4275 patients and 5735 controls were included. Overall, a significant association was found between the XPG Asp1104His polymorphism and the risk of gastrointestinal cancers (dominant model: OR = 1.15, 95% CI: 1.05-1.26; His/His vs. Asp/Asp: OR = 1.15, 95% CI: 1.01-1.32). When the analysis was stratified by ethnicity, similar results were observed in Asians under homozygote model; in stratification analysis by cancer type, increased cancer risk was detected in colorectal and hepatocellular carcinoma, but not for other gastrointestinal cancers. Furthermore, in subgroup analysis by source of control, we failed to detect any association among population, hospital and family-based populations. This meta-analysis indicated that the XPG Asp1104His polymorphism may be a risk factor for gastrointestinal cancers, especially of colorectal cancer.

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