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1.
Eur J Radiol ; 126: 108927, 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-32146345

RESUMO

PURPOSE: Portal venous pressure (PVP) measurement is of clinical significance, especially in patients with portal hypertension. However, the invasive nature and associated complications limits its application. The aim of the study is to propose a noninvasive predictive model of PVP values based on CT-extracted radiomic features. METHODS: Radiomics PVP (rPVP) models based on liver, spleen and combined features were established on an experimental cohort of 169 subjects. Radiomics features were extracted from each ROI and reduced via the LASSO regression to achieve an optimal predictive formula. A validation cohort of 62 patients treated for gastroesophageal varices (GOV) was used to confirm the utility of rPVP in predicting variceal recurrence. The association between rPVP and response to treatment was observed. RESULTS: Three separate predictive formula for PVP were derived from radiomics features. rPVP was significantly correlated to patient response to endoscopic treatment for GOV. Among which, the model containing both liver and spleen features has the highest predictability of variceal recurrence, with an optimal cut-off value at 29.102 mmHg (AUC 0.866). A Kaplan Meier analysis further confirmed the difference between patients with varying rPVP values. CONCLUSION: PVP values can be accurately predicted by a non-invasive, CT derived radiomics model. rPVP serves as a non-invasive and precise reference for predicting treatment outcome for GOV secondary to portal hypertension.

2.
ACS Nano ; 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32057223

RESUMO

As one of the major air pollutants, NOX is rather challenging to remove. The main treatment method is catalytic reduction with plenty of reducing agents, which lacks any effective control in an open air environment such as urban spaces. It is necessary to seek a self-powered electrochemical process for environmental treatment. The triboelectric nanogenerator (TENG), a developing technology with various advantages, is widely used in energy and environmental monitoring and cleaning. In this work, a radial-engine-shaped TENG system with five stacked TENGs is designed to synchronously absorb NOX and degrade its main enrichment forms of nitrate and nitrite in aqueous solution. In addition, the system possesses inherent phase differences and outputs continuous direct current after rectification. Moreover, we demonstrated that, driven by artificial wind at a speed of 6 m/s, the NOX generated by a chemical method was effectively degraded by the radial-engine-shaped TENG system.

3.
Cancer Genet ; 241: 25-33, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31964588

RESUMO

Recently, a number of long noncoding RNAs (lncRNAs) have been reported to play significant roles in human tumorigenesis. However, only few gastric cancer related lncRNAs have been well characterized. Here, we identified one lncRNA HRCEG, whose expression was decreased in the gastric cancer tissues compared with adjacent normal tissues. Overexpression of HRCEG significantly promoted cell apoptosis and inhibited cell proliferation. Importantly, we demonstrated that HRCEG levels inversely correlated with EMT process and HRCEG was regulated by the histone deacetylase 1 (HDAC1) in gastric cancer. These findings suggest that HRCEG might be regulated by HDAC1 to inhibit gastric cancer progress and metastatic capability via EMT pathway.

4.
Arthritis Rheumatol ; 2020 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-31930717

RESUMO

OBJECTIVE: GWASs have identified many susceptibility loci for systemic lupus erythematosus (SLE). However, most of these loci are located in noncoding regions of the genome. Long noncoding RNAs (lncRNAs) are pervasively expressed and reported to be involved in various diseases. This study aimed to explore the genetic significance of lncRNAs in SLE. METHODS: We performed a genome-wide survey of SLE risk variants in lncRNA gene loci among Han Chinese (4,556 SLE and 9,451 controls). The functional relevance of a risk variant in a lncRNA gene was explored using biochemical and molecular cell biology analyses. In vitro loss and gain of function strategies were performed to clarify the functional and phenotypic relevance of the susceptibility lncRNA. Moreover, correlation of this lncRNA with apoptosis were evaluated in SLE patients. RESULTS: We identified a new susceptibility locus in a lncRNA gene which we named SLEAR (rs13259960, Pcombined =1.03×10-11 , OR=1.35). The A>G variation at rs13259960, located in an intronic enhancer, impairs STAT1 recruitment to the enhancer that loops to the SLEAR promoter, resulting in decreased SLEAR level (3 G/G, 22 A/G, 103 A/A at rs13259960; P=0.0241). Moreover, SLEAR interacts with ILF2, hnRNP F and TAF15 to form a complex for transcriptional activation of the downstream anti-apoptotic genes. SLEAR regulates apoptosis in vitro and its expression level is correlated with cell death in SLE patients (n=30, r=0.824, P=2.15E-8). CONCLUSION: These findings suggest a mechanism by which the risk variant at rs13259960 modulates SLEAR expression and predisposes to SLE and may give insights into the SLE etiology.

5.
Bioinformatics ; 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31794005

RESUMO

MOTIVATION: RNA 5-methylcytosine (m5C) is a type of post-transcriptional modification that may be involved in numerous biological processes and tumorigenesis. RNA m5C can be profiled at single-nucleotide resolution by high-throughput sequencing of RNA treated with bisulfite (RNA-BisSeq). However, the exploration of transcriptome-wide profile and potential function of m5C in splicing remains to be elucidated due to lack of isoform level m5C quantification tool. RESULTS: We developed a computational package to quantify Epitranscriptomal RNA m5C at the transcript isoform level (named Episo). Episo consists of three tools, mapper, quant and Bisulfitefq, for mapping, quantifying, and simulating RNA-BisSeq data, respectively. The high accuracy of Episo was validated using an improved m5C-specific methylated RNA immunoprecipitation (meRIP) protocol, as well as a set of in silico experiments. By applying Episo to public human and mouse RNA-BisSeq data, we found that the RNA m5C is not evenly distributed among the transcript isoforms, implying the m5C may subject to be regulated at isoform level. AVAILABILITY: Episo is released under the GNU GPLv3+ license. The resource code Episo is freely accessible from https://github.com/liujunfengtop/Episo (with Tophat/cufflink) and https://github.com/liujunfengtop/Episo/tree/master/Episo_Kallisto (with Kallisto). SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

6.
Opt Express ; 27(21): 30909-30918, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31684332

RESUMO

Aluminum (Al) plasmonic nanostructures have recently demonstrated remarkable optical nonlinear phenomena, such as enhanced second harmonic (SH) generation. However, the relatively weak field enhancement resulted from large optical losses associated with aluminum nanostructures in combination with the difficulties in controlling the emission polarization pose as a challenge for SH enhancement and tuning. In this paper, we show that the SH emission of aluminum nanostructures can be efficiently enhanced with the polarization properties simultaneously tunable by using metal-insulator-metal (MIM) nanostructures, constituting of Al cross nanoantenna arrays on top of Al mirrors with a SiO2 spacing layer. Specifically, femtosecond laser beam with a linear polarization parallel to one arm illuminates on the structure while the orthogonal arms were physically modified by the laser-induced photothermal reshaping technique to control the SH radiation by the plasmonic resonances. Under the resonance at the SH wavelength, we observed one order of magnitude larger emission enhancement compared to that at the off-resonant condition. Interestingly, the polarization states can be well manipulated simultaneously by controlling the resonances of the orthogonal arms. The enhanced SH conversion and tunable polarization states pave the way for the development of nonlinear optical sources and advanced functional metasurfaces.

7.
Nat Commun ; 10(1): 5147, 2019 11 26.
Artigo em Inglês | MEDLINE | ID: mdl-31772189

RESUMO

In the new era of internet of things, big data collection and analysis based on widely distributed intelligent sensing technology is particularly important. Here, we report a flexible and durable wood-based triboelectric nanogenerator for self-powered sensing in athletic big data analytics. Based on a simple and effective strategy, natural wood can be converted into a high-performance triboelectric material with excellent mechanical properties, such as 7.5-fold enhancement in strength, superior flexibility, wear resistance and processability. The electrical output performance is also enhanced by more than 70% compared with natural wood. A self-powered falling point distribution statistical system and an edge ball judgement system are further developed to provide training guidance and real-time competition assistance for both athletes and referees. This work can not only expand the application area of the self-powered system to smart sport monitoring and assisting, but also promote the development of big data analytics in intelligent sports industry.

8.
J Cancer Res Ther ; 15(4): 766-772, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31436230

RESUMO

Background/Aim: Percutaneous thermal ablation combined with transarterial chemoembolization (TACE) becomes a treatment option for unresectable hepatocellular carcinoma (HCC). This study aims to investigate the safety and feasibility of percutaneous thermal ablation combined with simultaneous TACE for patients with HCC ≤ 5 cm. Materials and Methods: From June 2010 to February 2017, a total of 280 patients with HCC ≤ 5 cm who underwent percutaneous thermal ablation combined with simultaneous TACE were included in our study. Their clinical data were collected and analyzed. Results: Major complications occurred in five cases (1.8%). The complete necrosis rate was 91.9%. The median overall survival (OS) was 66.5 months (95% confidence interval [CI] = 57.7-75.2). The OS rates in 1-, 3-, 5-, and 7-year were 96.7%, 76.0%, 59.7%, and 31.1%, respectively. Tumor size (hazard ratio = 1.826; 95% CI = 1.131-2.947; P = 0.014) was considered as independent prognostic factors of long-term survival. Conclusion: Percutaneous thermal ablation combined with simultaneous TACE is a safe and effective treatment for HCC ≤ 5 cm.


Assuntos
Carcinoma Hepatocelular/terapia , Ablação por Cateter/mortalidade , Quimioembolização Terapêutica/mortalidade , Hipertermia Induzida/mortalidade , Neoplasias Hepáticas/terapia , Recidiva Local de Neoplasia/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma Hepatocelular/patologia , Terapia Combinada , Feminino , Seguimentos , Humanos , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/patologia , Estudos Retrospectivos , Taxa de Sobrevida , Resultado do Tratamento
9.
EMBO J ; 38(17): e101110, 2019 09 02.
Artigo em Inglês | MEDLINE | ID: mdl-31334575

RESUMO

Hepatocellular carcinoma (HCC) is the most prevalent liver cancer, characterized by a high rate of recurrence and heterogeneity. Liver cancer stem cells (CSCs) may well contribute to both of these pathological properties, but the mechanism underlying their self-renewal maintenance is poorly understood. Here, we identified a long noncoding RNA (lncRNA) termed HAND2-AS1 that is highly expressed in liver CSCs. Human HAND2-AS1 and its mouse ortholog lncHand2 display a high level of conservation. HAND2-AS1 is required for the self-renewal maintenance of liver CSCs to initiate HCC development. Mechanistically, HAND2-AS1 recruits the INO80 chromatin-remodeling complex to the promoter of BMPR1A, thereby inducing its expression and leading to the activation of BMP signaling. Importantly, interfering with expression of HAND2-AS1 by antisense oligonucleotides (ASOs) and BMPR1A by siRNAs has synergistic anti-tumorigenic effects on humanized HCC models. Moreover, knockout of lncHand2 or Bmpr1a in mouse hepatocytes impairs BMP signaling and suppresses the initiation of liver cancer. Our findings reveal that HAND2-AS1 promotes the self-renewal of liver CSCs and drives liver oncogenesis, offering a potential new target for HCC therapy.


Assuntos
Carcinoma Hepatocelular/genética , Neoplasias Hepáticas/genética , Células-Tronco Neoplásicas/química , RNA Longo não Codificante/genética , Transdução de Sinais , ATPases Associadas a Diversas Atividades Celulares/genética , Animais , Receptores de Proteínas Morfogenéticas Ósseas Tipo I/genética , Proteínas Morfogenéticas Ósseas/metabolismo , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Autorrenovação Celular , Proteínas de Ligação a DNA/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Camundongos , Transplante de Neoplasias , Células-Tronco Neoplásicas/patologia , Regulação para Cima
10.
Genome Res ; 29(9): 1521-1532, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31315906

RESUMO

Long noncoding RNAs (lncRNAs) can regulate the activity of target genes by participating in the organization of chromatin architecture. We have devised a "chromatin-RNA in situ reverse transcription sequencing" (CRIST-seq) approach to profile the lncRNA interaction network in gene regulatory elements by combining the simplicity of RNA biotin labeling with the specificity of the CRISPR/Cas9 system. Using gene-specific gRNAs, we describe a pluripotency-specific lncRNA interacting network in the promoters of Sox2 and Pou5f1, two critical stem cell factors that are required for the maintenance of pluripotency. The promoter-interacting lncRNAs were specifically activated during reprogramming into pluripotency. Knockdown of these lncRNAs caused the stem cells to exit from pluripotency. In contrast, overexpression of the pluripotency-associated lncRNA activated the promoters of core stem cell factor genes and enhanced fibroblast reprogramming into pluripotency. These CRIST-seq data suggest that the Sox2 and Pou5f1 promoters are organized within a unique lncRNA interaction network that determines the fate of pluripotency during reprogramming. This CRIST approach may be broadly used to map lncRNA interaction networks at target loci across the genome.


Assuntos
Cromatina/genética , Fator 3 de Transcrição de Octâmero/genética , RNA Longo não Codificante/genética , Fatores de Transcrição SOXB1/genética , Análise de Sequência de RNA/métodos , Animais , Sistemas CRISPR-Cas , Linhagem Celular , Reprogramação Celular , Fibroblastos/citologia , Fibroblastos/metabolismo , Camundongos , Células-Tronco Pluripotentes/citologia , Células-Tronco Pluripotentes/metabolismo , Regiões Promotoras Genéticas , Sequências Reguladoras de Ácido Nucleico
11.
Nat Commun ; 10(1): 3391, 2019 07 29.
Artigo em Inglês | MEDLINE | ID: mdl-31358770

RESUMO

Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of the metabolic syndrome that elevates the risk of hepatocellular carcinoma (HCC). Although alteration of lipid metabolism has been increasingly recognized as a hallmark of cancer cells, the deregulated metabolic modulation of HCC cells in the NAFLD progression remains obscure. Here, we discovers an endoplasmic reticulum-residential protein, Nogo-B, as a highly expressed metabolic modulator in both murine and human NAFLD-associated HCCs, which accelerates high-fat, high-carbohydrate diet-induced metabolic dysfunction and tumorigenicity. Mechanistically, CD36-mediated oxLDL uptake triggers CEBPß expression to directly upregulate Nogo-B, which interacts with ATG5 to promote lipophagy leading to lysophosphatidic acid-enhanced YAP oncogenic activity. This CD36-Nogo-B-YAP pathway consequently reprograms oxLDL metabolism and induces carcinogenetic signaling for NAFLD-associated HCCs. Targeting the Nogo-B pathway may represent a therapeutic strategy for HCC arising from the metabolic syndrome.


Assuntos
Autofagia , Carcinoma Hepatocelular/metabolismo , Lipoproteínas LDL/metabolismo , Neoplasias Hepáticas/metabolismo , Proteínas Nogo/metabolismo , Hepatopatia Gordurosa não Alcoólica/metabolismo , Animais , Carcinoma Hepatocelular/complicações , Carcinoma Hepatocelular/genética , Linhagem Celular Tumoral , Dieta Hiperlipídica/efeitos adversos , Retículo Endoplasmático/metabolismo , Feminino , Perfilação da Expressão Gênica/métodos , Células Hep G2 , Humanos , Estimativa de Kaplan-Meier , Neoplasias Hepáticas/complicações , Neoplasias Hepáticas/genética , Síndrome Metabólica/etiologia , Síndrome Metabólica/genética , Síndrome Metabólica/metabolismo , Camundongos Endogâmicos C57BL , Camundongos Nus , Proteínas Nogo/genética , Hepatopatia Gordurosa não Alcoólica/complicações , Hepatopatia Gordurosa não Alcoólica/genética , Transdução de Sinais/genética , Transplante Heterólogo
12.
Clin Sci (Lond) ; 133(13): 1487-1503, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31278132

RESUMO

Accumulating evidences indicate that circular RNAs (circRNAs) play a vital role in diverse cancer biology. However, the contributions of circRNAs to hepatocellular carcinoma (HCC) and their underlying mechanism remain largely unknown. The present study aims at investigating the role of circRNA-104718 in HCC progression, which has been observed to be significantly up-regulated in HCC tissues. We found that, higher expression of circRNA-104718 also leds to a poor prognosis in HCC patients. Using luciferase binding assays and RNA immunoprecipitation studies, we identified circRNA-104718 is physically associated and co-expressed with microRNA (miR)-218-5p in HCC. Mechanistically, we demonstrated that circRNA-104718 functions as a competing endogenous RNAs (ceRNAs) and competes with thioredoxin domain-containing protein 5 (TXNDC5) mRNA and directly binds to miR-218-5p. Functionally, we found that ectopically expressed circRNA-104718 accelerated cell proliferation, migration, invasion, and inhibited apoptosis. In vivo studies on a nude mice model showed that circRNA-104718 overexpression could increase the tumor size and the rate of metastasis. Silencing of circRNA-104718 could decrease both the tumor size and metastasis significantly. Conversely, we also observed overexpression of miR-218-5p could in turn decrease the proliferation, migration, invasion, and increase apoptosis. Furthermore, circRNA-104718 could relieve the suppression of miR-218-5p target TXNDC5 and thereby cause an inhibition of miR's functions. In summary, our results indicate that circRNA-104718 acts as a ceRNA and promotes HCC progression through the targeting of miR-218-5p/TXNDC5 signaling pathway. Thus, we propose that circRNA-104718 would be a promising target for HCC diagnosis and therapy.

13.
Biomed Res Int ; 2019: 6265183, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31143774

RESUMO

Purpose: The aim of this study was to evaluate the safety and efficacy of transcatheter arterial embolization (TAE) in patients with renal hemorrhage after percutaneous nephrolithotomy (PCNL) and evaluate the risk factors that may result in severe bleeding requiring TAE. Methods: We retrospectively reviewed 121 patients with post-PCNL renal hemorrhage. Thirty-two patients receiving endovascular embolization were compared with 89 patients only receiving conservative treatment. The demographic and clinical data were recorded and compared between the two groups. The values of estimated glomerular filtration rate (eGFR) and serum creatinine (SCr) were recorded preoperatively, postoperatively, and at last follow-up and analyzed to evaluate the safety and efficiency of TAE. Results: The successful hemostasis rate of conservative therapy was 73.6% (89/121) and that of TAE was 100% (32/32). SCr and eGFR were not significantly different before PCNL and after the last follow-up of TAE (SCr: 0.95 vs. 0.95 mg/dl, P=0.857; eGFR: 86.77 vs. 86.18 ml/min/1.73m2, P=0.715). The univariate analysis demonstrated that advanced age, urinary tract infection, and diabetes mellitus were significantly associated with severe bleeding during PCNL. Multivariate analysis further identified that diabetes mellitus was an independent risk factor for severe bleeding needing TAE [odds ratio (OR): 3.778, 95% confidence interval (CI):1.276-11.190, and P=0.016]. Conclusion: TAE is a safe and effective procedure to treat renal hemorrhage that cannot be resisted by conservative treatment after PCNL. Diabetes mellitus was associated with high risks of severe bleeding needing TAE after PCNL.


Assuntos
Cateterismo , Embolização Terapêutica , Hemorragia/terapia , Nefrolitotomia Percutânea , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Artéria Renal/diagnóstico por imagem , Resultado do Tratamento
14.
Nat Commun ; 10(1): 2309, 2019 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-31127107

RESUMO

A high-speed micromotor is usually actuated by a power source with high voltage and frequency. Here we report a triboelectric micromotor by coupling a micromotor and a triboelectric nanogenerator, in which the micromotor can be actuated by ultralow-frequency mechanical stimuli. The performances of the triboelectric micromotor are exhibited at various structural parameters of the micromotor, as well as at different mechanical stimuli of the triboelectric nanogenerator. With a sliding range of 50 mm at 0.1 Hz, the micromotor can start to rotate and reach over 1000 r min-1 at 0.8 Hz. The maximum operation efficiency of the triboelectric micromotor can reach 41%. Additionally, the micromotor is demonstrated in two scanning systems for information recognition. This work has realized a high-speed micromotor actuated by ultralow frequency mechanical stimuli without an external power supply, which has extended the application of triboelectric nanogenerator in micro/nano electromechanical systems, intelligent robots and autonomous driving.

15.
ACS Nano ; 13(5): 5306-5325, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-31018094

RESUMO

Engineering a versatile oncotherapy nanoplatform integrating both diagnostic and therapeutic functions has always been an intractable challenge in targeted cancer treatment. Herein, to actualize the theme of precise medicine, a nanoplatform is developed by anchoring Mn-Cdots to doxorubicin (DOX)-loaded mesoporous silica-coated gold cube-in-cubes core/shell nanocomposites and further conjugating them to a Arg-Gly-Asp (RGD) peptide (denoted as RGD-CCmMC/DOX) to achieve an active-targeting effect. Under 635 nm irradiation, the nanoplatform acts as oxygen nanogenerator that produces O2 in situ and amplifies the content of singlet oxygen (1O2) in the hypoxic tumor microenvironment (TME), which has been demonstrated to attenuate tumor hypoxia and synchronously enhance photodynamic efficacy. Moreover, the gold cube-in-cube core in this work has been proven as a photothermal agent for hyperthermia, which exhibits a favorable photothermal effect with a 65.6% calculated photothermal conversion efficiency under 808 nm irradiation. In addition, the nanoplatform achieves heat- and pH-sensitive drug release with precise control to specific-tumor sites, executing combined chemo-phototherapy functions. Besides, it functions as a multimodal bioimaging agent of photothermal, fluorescence, and magnetic resonance imaging for the accurate diagnosis and guidance of therapy. As validated by in vivo and in vitro assays, the TME-responsive nanoplatform is highly biocompatible and effectively obliterates 4T1 tumor xenografts on nude mice after triple-synergetic treatment. This work presents a rational design of versatile nanoplatforms, which modulate the TME to enable high therapeutic performance and multiplexed imaging, which provides an innovative paradigm for targeted tumor therapy.

16.
Eur J Obstet Gynecol Reprod Biol ; 236: 177-182, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30943448

RESUMO

OBJECTIVE(S): CHL1 (close homologue of L1 or cell adhesion molecule L1 like), also referred as CALL, is a member of the L1 gene family of neural cell adhesion molecules and belongs to immunoglobulin superfamily. This study aims to investigate the potential correlation of the CHL1 gene and the long non-coding RNAs (lncRNAs), i.e., CHL1-AS1 and CHL1-AS2, and to validate the expression patterns of CHL1 and CHL1-AS2 in ovarian endometriosis (EM). STUDY DESIGN: Our previous microarray analyses (GSE86534) of 4 patients with ovarian EM indicated that CHL1 was the most upregulated mRNA in ectopic endometrium (EC) compared with eutopic endometrium (EU) tissues, and that its two antisense lncRNAs CHL1-AS1 and CHL1-AS2, exhibited the same expression pattern. We used a bioinformatics-based strategy to calculate the correlation among CHL1, CHL1-AS1 and CHL1-AS2. Gene set enrichment analysis (GSEA) was performed to analyze commonly enriched gene sets for CHL1-AS1 and CHL1-AS2. Using quantitative real-time polymerase chain reaction (qPCR), we examined the expression levels of CHL1 mRNA and lncRNA CHL1-AS2 in paired tissues of EC and EU from 30 EM patients and normal endometrium (NE) tissues from 27 controls using quantitative real-time polymerase chain reaction (qPCR). We also examined the expression of CHL1 protein in EC, EU and NE tissues using western blotting and immunohistochemistry (IHC). RESULTS: CHL1, CHL1-AS1 and CHL1-AS2 were significantly correlated with each other given that the Pearson correlation values were > 0.9 using bioinformatic calculation. GSEA revealed that CHL1-AS1 and CHL1-AS2 were negatively associated with the same gene set "WAMUNYOKOLI_OVARIAN_CANCER_LMP". qPCR confirmed that the CHL1 and CHL1-AS2 expression levels were significantly higher in EC tissues than in EU and NE tissues, while they were not significantly different in EU compared with NE tissues. The relative expression levels of CHL1 and CHL1-AS2 in EC compared with EU tissues were positively significantly correlated (Pearson correlation coefficient = 0.421 and P value = 0.02). Elevated expression of CHL1 protein in EC tissues was detected by western blotting. IHC revealed that CHL1 protein expression levels enhanced in ectopic endometrial glands and stroma. CONCLUSION(S): Our results indicate a significant correlation among CHL1, CHL1-AS1 and CHL1-AS2, which might be involved in the development of ovarian EM and serve as novel targets for future research.


Assuntos
Moléculas de Adesão Celular/metabolismo , Endometriose/metabolismo , Doenças Ovarianas/metabolismo , RNA Longo não Codificante/metabolismo , Regulação para Cima , Adulto , Moléculas de Adesão Celular/genética , Endometriose/genética , Endométrio/metabolismo , Feminino , Humanos , Pessoa de Meia-Idade , Doenças Ovarianas/genética , Ovário/metabolismo , RNA Longo não Codificante/genética
17.
J Hematol Oncol ; 12(1): 19, 2019 02 22.
Artigo em Inglês | MEDLINE | ID: mdl-30795783

RESUMO

BACKGROUND: Increasing evidence has demonstrated that mesenchymal stem cells (MSCs) play a role in the construction of tumor microenvironments. Co-culture between tumor cells and MSCs provides an easy and useful platform for mimicking tumor microenvironments and identifying the important members involved in tumor progress. The long non-coding RNAs (lncRNAs) have been shown to regulate different tumorigenic processes. In this study, we aimed to examine functional lncRNA deregulations associated with breast cancer malignancy instigated by MSC-MCF-7 co-culture. METHODS: The microarrays were used to profile the expression changes of lncRNAs in MCF-7 cells during epithelial-mesenchymal transition (EMT) induced by co-culture with MSCs. We found that an intergenic lncRNA KB-1732A1.1 (termed LincK, partly overlapped with GASL1) was significantly elevated. To investigate the biological function of LincK, the expression of EMT markers, cell migration, invasion, proliferation, and colony formation were evaluated in vitro and xenograft assay in nude mice were performed in vivo. Furthermore, we detected LincK expression in clinical samples using RNAscope® technology and verified aberrant expression of LincK in breast cancer data sets from The Cancer Genome Atlas (TCGA) by bioinformatic analysis. The underlying mechanisms of LincK were investigated using mRNA microarray analyses, Western blot, RNA pull down, and RNA immunoprecipitation. RESULTS: LincK induced an EMT progress in breast cancer cells (BCC) MCF-7, MDA-MB-453, and MDA-MB-231. The depletion of LincK decreased the growth, migration, and invasion in BCC, whereas the overexpression of LincK exerted the opposite effects. Moreover, knockdown of LincK repressed tumorigenesis, and ectopic expression of LincK promoted tumor growth in MCF-7 xenograft model. LincK ablation in MDA-MB-231 cells dramatically impaired lung metastasis when incubated intravenously into nude mice. Further, LincK was frequently elevated in breast cancer compared with normal breast tissue in clinical samples. Mechanistically, LincK may share common miRNA response elements with PBK and ZEB1 and regulate the effects of miR-200 s. CONCLUSION: LincK plays a significant role in regulating EMT and tumor growth and could be a potential therapeutic target in breast cancer.

18.
Opt Lett ; 44(4): 727-730, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30767972

RESUMO

Based on the Richards-Wolf vectorial diffraction theory and inverse Faraday effect, we first propose a scheme to generate three-dimensional magnetization needle (MN) arrays with arbitrary orientation for each individual needle and controllable spatial position and number by reversing the electric dipole array radiation. To achieve this, each unit of the electric dipole array has two electric dipoles with orthogonal oscillation directions and quadrature phase and is located mirror-symmetric with respect to the focal plane of the high numerical aperture lens. Uniformly distributed MNs with a subwavelength lateral size of 0.44λ and a longitudinal depth of 5.36λ with four different orientations are obtained by optimized arrangement for 2N (here, N=2) units of the electric dipole array. The corresponding purity of MNs is also discussed in detail. Furthermore, two combinations of MN arrays with orthogonal orientation are emphatically exploited in the hybrid bit-patterned media recording. The results illustrate the richness of the proposed methods to locally control the particular orientation properties of the MN and find many potential applications in multichannel/multilayer magneto-optical storage, information security, and spintronics.

19.
Hepatol Res ; 49(5): 540-549, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30597744

RESUMO

AIM: To assess the diagnostic accuracy of liver and spleen stiffness measured by 2-D shear-wave elastography (SWE) in evaluation of clinically significant and severe portal hypertension (CSPH and SPH, respectively). METHODS: Clinical data of 155 hepatitis B-related cirrhosis patients and their liver and spleen stiffness (L-SWE and S-SWE, respectively) were collected. The diagnostic performances of L-SWE, S-SWE, the liver stiffness-spleen diameter to platelet ratio score (LSPS) and portal hypertension risk score were evaluated. RESULTS: One hundred and four patients were eligible for analysis. Clinically significant and severe PH were detected in 84 and 74 patients, respectively. Liver and spleen stiffness were significantly correlated with hepatic venous pressure gradient in overall, CSPH, and SPH groups (rL = 0.607, 0.554, and 0.412; rS = 0.665, 0.566, and 0.467, respectively; all P < 0.05). The area under the receiver operating characteristic curves of L-SWE, S-SWE, LSPS, and PH risk score were 0.72 (95% confidence interval [CI], 0.49-0.95), 0.81 (95% CI, 0.55-0.97), 0.76 (95% CI, 0.51-0.96), and 0.73 (95% CI, 0.55-0.88) for CSPH, and 0.77 (95% CI, 0.51-0.93), 0.85 (95% CI, 0.59-0.96), 0.80 (95% CI, 0.58-0.98), and 0.80 (95% CI, 0.59-0.93) for SPH. The best cut-off of L-SWE for determining CSPH and SPH were 16.1 kPa (sensitivity, 78%; specificity, 72%) and 23.5 kPa (sensitivity, 81%; specificity, 79%). For S-SWE, the best cut-offs were 25.3 kPa (sensitivity, 85%; specificity, 79%) and 33.4 kPa (sensitivity, 74%; specificity, 70%). A cut-off of L-SWE <13.2 kPa or S-SWE <23.2 kPa was able to rule out CSPH, whereas a cut-off of L-SWE >24.9 kPa or S-SWE >34.2 kPa was able to rule in CSPH. CONCLUSIONS: Liver and spleen stiffness measured by 2-D SWE are reliable and promising non-invasive parameters to assess CSPH and SPH.

20.
IEEE Trans Cybern ; 49(11): 4004-4016, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30072354

RESUMO

This paper investigates a novel leader-following attitude control approach for spacecraft formation under the preassigned two-layer performance with consideration of unknown inertial parameters, external disturbance torque, and unmodeled uncertainty. First, two-layer prescribed performance is preselected for both the attitude angular and angular velocity tracking errors. Subsequently, a distributed two-layer performance controller is devised, which can guarantee that all the involved closed-loop signals are uniformly ultimately bounded. In order to tackle the defect of statically two-layer performance controller, learning-based control strategy is introduced to serve as an adaptive supplementary controller based on adaptive dynamic programming technique. This enhances the adaptiveness of the statically two-layer performance controller with respect to unexpected uncertainty dramatically, without any prior knowledge of the inertial information. Furthermore, by employing the robustly positively invariant theory, the input-to-state stability is rigorously proven under the designed learning-based distributed controller. Finally, two groups of simulation examples are organized to validate the feasibility and effectiveness of the proposed distributed control approach.

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