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1.
J Cell Physiol ; 236(11): 7711-7724, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34018612

RESUMO

Airway epithelial cells, the first barrier of the respiratory tract, play an indispensable role in innate immunity. Integrin ß4 (ITGB4) is a structural adhesion molecule that is involved in the pathological progression of acute inflammatory diseases and is downregulated in asthmatic patients. Research has shown that endothelial ITGB4 has proinflammatory properties in acute lung injury (ALI). However, the role of epithelial ITGB4 in a murine ALI model is still unknown. This study investigated the role of ITGB4 in lipopolysaccharide (LPS)-induced ALI. We found that ITGB4 in the airway epithelium had remarkably increased after the introduction of LPS in vivo and in vitro. Then, we constructed airway epithelial cell-specific ITGB4 knockout (ITGB4-/- ) mice to study its role in ALI. At a time point of 12 h after the tracheal injection of LPS, ITGB4-/- mice showed increased macrophages (mainly M1-type macrophages) and neutrophil infiltration into the lungs; inflammation-related proteins including interleukin (IL)-6, tumor necrosis factor, and IL-17A were significantly elevated compared to their levels in ITGB4+/+ mice. Furthermore, we investigated the role of ITGB4 in the anti-inflammatory response. Intriguingly, in the ITGB4-/- + LPS group, we found significantly reduced expression of anti-inflammatory factors, including IL-10 messenger RNA (mRNA) and ARG-1 mRNA. We also observed that monocyte chemotactic protein (MCP-1) increased significantly both in vivo and in vitro. Airway epithelium activates macrophages, most likely driven by MCP-1, which we confirmed in the coculture of epithelia and macrophages. These phenomena indicate that ITGB4 in airway epithelial cells plays an important role in the process of inflammation and activation of macrophages in ALI. Overall, these data demonstrated a novel link between airway epithelial ITGB4 and the inflammatory response in LPS-induced ALI.

2.
BMC Cancer ; 21(1): 22, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33402129

RESUMO

BACKGROUND: An accurate intraoperative prediction of lymph node metastatic risk can help surgeons in choosing precise surgical procedures. We aimed to develop and validate nomograms to intraoperatively predict patterns of regional lymph node (LN) metastasis in patients with esophageal cancer. METHODS: The prediction model was developed in a training cohort consisting of 487 patients diagnosed with esophageal cancer who underwent esophagectomy with complete LN dissection from January 2016 to December 2016. Univariate and multivariable logistic regression were used to identify independent risk factors that were incorporated into a prediction model and used to construct a nomogram. Contrast-enhanced computed tomography reported LN status and was an important comparative factor of clinical usefulness in a validation cohort. Nomogram performance was assessed in terms of calibration, discrimination, and clinical usefulness. An independent validation cohort comprised 206 consecutive patients from January 2017 to December 2017. RESULTS: Univariate analysis and multivariable logistic regression revealed three independent predictors of metastatic regional LNs, three independent predictors of continuous regional LNs, and two independent predictors of skipping regional LNs. Independent predictors were used to build three individualized prediction nomograms. The models showed good calibration and discrimination, with area under the curve (AUC) values of 0.737, 0.738, and 0.707. Application of the nomogram in the validation cohort yielded good calibration and discrimination, with AUC values of 0.728, 0.668, and 0.657. Decision curve analysis demonstrated that the three nomograms were clinically useful in the validation cohort. CONCLUSION: This study presents three nomograms that incorporate clinicopathologic factors, which can be used to facilitate the intraoperative prediction of metastatic regional LN patterns in patients with esophageal cancer.


Assuntos
Adenocarcinoma/secundário , Neoplasias Esofágicas/patologia , Carcinoma de Células Escamosas do Esôfago/secundário , Linfonodos/patologia , Nomogramas , Adenocarcinoma/diagnóstico por imagem , Adenocarcinoma/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Área Sob a Curva , Neoplasias Esofágicas/diagnóstico por imagem , Neoplasias Esofágicas/cirurgia , Carcinoma de Células Escamosas do Esôfago/diagnóstico por imagem , Carcinoma de Células Escamosas do Esôfago/cirurgia , Feminino , Seguimentos , Humanos , Linfonodos/diagnóstico por imagem , Linfonodos/cirurgia , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Tomografia Computadorizada por Raios X
3.
Materials (Basel) ; 13(7)2020 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-32260498

RESUMO

Polypropylene (PP) is notch sensitive and brittle under severe conditions of deformation, limiting wider range of its usage as a structural load-bearing polymer. Hence, in this work the magnesium borate whisker (MBw), with similar mechanical properties to carbon fiber but much less expensive than polycrystalline silicon carbide, was modified by boric acid ester (BAE) and then used to fabricate PP composites. The mechanical properties, morphology, and non-isothermal crystallization property of virgin PP, PP/MBw, and PP/BAE-MBw composites were studied through mechanical testing, scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively. The non-isothermal crystallization data was analyzed via Mo, Kissinger, and Dobreva methods. The results reveal that the incorporation of BAE-MBw into PP matrix results in higher tensile strength and impact strength than those of virgin PP and PP/MBw composite. The activation energies based on Kissinger were 190.20 kJ/mol for virgin PP, 206.59 kJ/mol for PP/MBw, and 218.98 kJ/mol for PP/BAE-MBw. The nucleation activities of whiskers determined by the Dobreva model were 0.86 for PP/MBw and 0.75 for PP/BAE-MBw. As a result, the whiskers, especially the modified whiskers, act as active substrates to facilitate heterogeneous nucleation, which leads to an increase in crystallization rate.

4.
ACS Appl Mater Interfaces ; 11(30): 27363-27370, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31287297

RESUMO

Generally, electrochemiluminescence (ECL) assays are performed in the presence of a coreactant. The addition of the coreactant in the detection solution would make the ECL system lack sufficient stability. In the case of dissolved oxygen as the coreactant, the unknown concentration of dissolved O2 would result in an inevitable error and a lack of reproducibility in detection. A coreactant-free ECL assay could overcome the above shortcomings and thus is an ideal choice. In this work, a coreactant-free dual amplified ECL strategy was constructed for ultrasensitive detection of microRNA (miRNA). Here, target-catalyzed hairpin assembly and enzyme-triggered DNA walker recycling amplification were integrated to achieve dual signal amplification. Carboxyl-functionalized poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1'-3}-thiadiazole)] (PFBT-COOH) dots were used as luminophores, which displayed prominent ECL performance without adding any coreactants and removing the dissolved O2. As a result, the detection of miRNA was achieved, and the linear range was from 10 aM to 5 pM, and the detection limit was low to 3.3 aM. Meanwhile, the practicability of our biosensor was investigated by analyzing the expression of miRNA in cell lysates. The PFBT-COOH dots provided a great platform for constructing coreactant-free ECL biosensors and expanded the application of conjugated polymer dots in clinical analysis.


Assuntos
Técnicas Biossensoriais , Técnicas Eletroquímicas , MicroRNAs/isolamento & purificação , Humanos , Limite de Detecção , Medições Luminescentes , MicroRNAs/química , Pontos Quânticos/química
5.
Thorac Cancer ; 10(8): 1692-1701, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31243884

RESUMO

The circRNA circAGFG1 is reported to be important in triple-negative breast cancer progression. However, the mechanism of circAGFG1 in non-small-cell lung cancer (NSCLC) remains unknown. In this study, expression of circAGFG1 was determined by real-time PCR in 20 pairs of NSCLC tissues and adjacent tissues. Next, functional experiments with circAGFG1 were performed in vitro to evaluate the role of circAGFG1 in tumor metastasis and growth. Meanwhile, a dual luciferase reporter assay, RNA pull-down and RNA immunoprecipitation experiments were used to explore the interaction between circAGFG1 and miR-203. Our results revealed that expression levels of circAGFG1 and miR-203 are upregulated in non-small-cell lung cancer tissues. CircAGFG1 enhances NSCLC cell proliferation, invasion, migration and epithelial-mesenchymal transition in vitro. Mechanistic analyses indicated that circAGFG1 acts as a sponge for miR-203 to repress the effect of miR-203 on its target, ZNF281. In conclusion, our study suggests that circAGFG1 promotes NSCLC growth and metastasis though a circAGFG1/miR-203/ZNF281 axis and may represent a novel therapeutic target.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/metabolismo , Neoplasias Pulmonares/metabolismo , MicroRNAs/metabolismo , Proteínas Repressoras/metabolismo , Células A549 , Sítios de Ligação , Carcinoma Pulmonar de Células não Pequenas/patologia , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Transição Epitelial-Mesenquimal , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patologia , MicroRNAs/genética , Metástase Neoplásica , Complexo de Proteínas Formadoras de Poros Nucleares/biossíntese , Complexo de Proteínas Formadoras de Poros Nucleares/genética , Proteínas de Ligação a RNA/biossíntese , Proteínas de Ligação a RNA/genética , Proteínas Repressoras/biossíntese , Proteínas Repressoras/genética , Transfecção , Regulação para Cima
6.
Chem Commun (Camb) ; 54(22): 2777-2780, 2018 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-29484320

RESUMO

An ultrasensitive electrochemiluminescence (ECL) detection for Cu2+ was explored using the carboxyl functionalized poly(9,9-dioctylfluorenyl-2,7-diyl) (PS-COOH-co-PFO) dots as the signal label without adding any coreactant.

7.
Medicine (Baltimore) ; 96(21): e7003, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28538413

RESUMO

RATIONALE: The optimal therapeutic regimen for primary malignant melanoma of the esophagus (PMME) need to be further elucidated. Besides, the efficacy of surgery for PMME with remote metastasis is uncertain for its rarity. PATIENT CONCERNS: Herein a previously healthy patient was admitted for dysphagia and fatigue, without significant weight loss. DIAGNOSES: The pathological and molecular tests revealed his diagnosis of BRAF-mutant, advanced PMME with localized pulmonary metastasis. INTERVENTIONS: Single-stage Ivor-Lewis esophagectomy and lobectomy were performed successfully, followed by 4 cycles of conventional chemotherapy, and concurrent high-dose interferon lasting for 1 year. OUTCOMES: The patient survived without logo-regional recurrence or remote metastasis during the follow up of two and a half years up to now. LESSONS: Timely resection of localized primary and metastatic lesions might deliver a chance to obtain better prognosis for selected PMME patients; however, high-quality trials with longer follow-up are needed.


Assuntos
Neoplasias Esofágicas/patologia , Neoplasias Esofágicas/cirurgia , Neoplasias Pulmonares/secundário , Neoplasias Pulmonares/cirurgia , Melanoma/patologia , Melanoma/cirurgia , Neoplasias Esofágicas/diagnóstico por imagem , Neoplasias Esofágicas/tratamento farmacológico , Esofagectomia , Humanos , Pulmão/diagnóstico por imagem , Pulmão/cirurgia , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/tratamento farmacológico , Masculino , Melanoma/diagnóstico por imagem , Melanoma/tratamento farmacológico , Pessoa de Meia-Idade
8.
J Thorac Dis ; 9(3): 849-859, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28449496

RESUMO

Esophageal cancer is the eighth most common cancer and the sixth leading cause of cancer-related death worldwide. Surgery is the primary form of treatment, but the survival is poor, especially for patients with locally advanced esophageal cancer. Radiotherapy has been a critical treatment option that may be combined with chemotherapy in patients with unresectable esophageal cancer. However, resistance to chemoradiotherapy might result in treatment failures and cancer relapse. This review will mainly focus on the possible cellular mechanisms and tumor-associated microenvironmental (TAM) factors that result in radioresistance in patients with esophageal cancer. In addition, current strategies to increase radiosensitivity, including targeted therapy and the use of radiosensitive biomarkers in clinical treatment, are discussed in this review.

9.
Zhonghua Wei Chang Wai Ke Za Zhi ; 16(12): 1180-2, 2013 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-24369401

RESUMO

OBJECTIVE: To retrospectively evaluate the efficacy of classic and modified Ivor-Lewis surgical procedure in the treatment of mid-low thoracic esophageal cancer. METHODS: Clinical data of 140 patients with middle-lower thoracic esophageal cancer undergoing modified Ivor-Lewis esophagectomy from March 2009 to April 2010 (modified group) and 112 patients with same disease undergoing classic Ivor-Lewis esophagectomy from April 2010 to April 2011 in our department were collected. Operative time, surgical complications, total number of harvested lymph node, distribution of lymph nodes, lymph node metastasis rate, as well as postoperative pathological stage were compared between two groups. RESULTS: There were no significant differences between two groups in general informations, operative time and surgical complications (P>0.05). The number of harvested superior mediastinum lymph nodes in classic Ivor-Lewis group was significantly more than that in modified group (8.0±2.1 vs. 3.1±0.6, P<0.05). Ratio of postoperative positive lymph node metastasis was significantly higher in classic Ivor-Lewis group as compared to modified group[41.1% (46/112) vs. 27.9% (39/140), P<0.05]. CONCLUSION: As compared to modified Ivor-Lewis procedure, classic Ivor-Lewis procedure is better in the resection of superior mediastinum lymph node and the evaluation of postoperative pathological stage, therefore it conforms better to the principle of cancer treatment of esophageal carcinoma.


Assuntos
Neoplasias Esofágicas/cirurgia , Esofagectomia/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
10.
Chemphyschem ; 14(9): 1969-76, 2013 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-23630164

RESUMO

The adsorption properties of water molecules on an MgSO4 (100) surface were investigated by using density functional theory (DFT) and supercell models. Optimized stable geometries of one and more than one water molecules adsorbed on an ideal MgSO4 (100) surface were obtained. The configurations with water molecules adsorbed on atoms of the second and third atomic layers of the MgSO4 (100) surface are quite stable. After adsorption, the separations between both the adjacent Mg atoms (R(Mg-Mg)) and the adjacent O atoms of the surface (R(O-O)) increase, which indicates that the MgSO4 (100) surface starts to deliquesce. In addition, water molecules are more likely to adsorb onto a defective surface rather than an ideal surface. Mulliken population analysis suggests that fewer charges transfer to the water molecule from the Mg atom of a defective substrate. Finally, Raman spectra were calculated for 0.5, 1, and 2 ML (ML=monolayer) water adsorbed on an MgSO4 (100) surface, which is helpful for further related experiments.

11.
J Colloid Interface Sci ; 393: 340-6, 2013 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-23207049

RESUMO

Density functional theory (DFT) calculations were applied to investigate the adsorption of water monomer, water clusters on NaNO(3)(001) surface. Single water molecule is more likely to locate on the bridge site with its H atom attracted by the O atom of nitrate ion and its O atom adjacent to Na(+). Mulliken population analysis shows that fewer electrons transfer from the Na atom of substrate to water molecule. A systematic study of water clusters adsorption at high coverages ranging from 0.5 monolayer (ML), 0.75 ML, 1 ML, 1.25 ML, and 1.5 ML on NaNO(3)(001) surface was also investigated, and the results indicate that for 1 ML water adsorption on NaNO(3)(001) surface, a water chain is formed among four water molecules through hydrogen bonds. Interestingly, the water molecules are linked through hydrogen bonds to form a 14-membered macrocyclic water ring for 1.5 ML adsorption on NaNO(3)(001) surface. Our estimated O-H symmetric stretching frequency (ν(O-H)) will have blueshift with decrease of water coverage, which is consistent with the tendency given by experiments.


Assuntos
Nitratos/química , Teoria Quântica , Adsorção , Propriedades de Superfície , Água/química
12.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 42(6): 842-5, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22332556

RESUMO

OBJECTIVE: To determine the expression and localization of transforming growth factor (TGF)-beta1 in condylar cartilage of adolescent rats following occlusion vertical rising procedures. METHODS: Forty male rats of 5 weeks of age were randomly divided into the experiment group (n=20) and the control group (n=20). The rats in the experiment group were fitted with upper posterior bite rising splints on their upper molar. Five rats from each group were killed at 7, 14, 21 and 28 days after the procedures, and their left condylar cartilages were taken for the determination of the expression of TGF-beta1 through SABC. RESULTS: The rats in the experiment group had significantly less expression of TGF-beta1 in condylar cartilage than the controls. The rats in the experiment group had the lowest TGF-beta17 day after the procedure. The level of TGF-beta1 increased over time. More TGF-beta1 positive cells were found in the rats of the experiment group than those in the control 28 days after the procedure. CONCLUSION: Occlusal vertical rising stimulates the expression of TGF-beta1 in condylar cartilage, which may be part of the adaptive remodeling of condylar cartilage in adolescent rats.


Assuntos
Cartilagem Articular/metabolismo , Oclusão Dentária , Côndilo Mandibular/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Animais , Cartilagem Articular/crescimento & desenvolvimento , Masculino , Côndilo Mandibular/crescimento & desenvolvimento , Distribuição Aleatória , Ratos , Ratos Wistar
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