Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 984
Filtrar
1.
J Recept Signal Transduct Res ; : 1-7, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32998602

RESUMO

BACKGROUND: Cell division control (CDC) 42 has been involved in the regulation of diverse cancers. Macrophage recruitment plays an important role in the pathogenesis and development of tumor. However, it remains unclear whether CDC42 contributes to macrophage recruitment and lung tumorigenesis in vivo. METHODS: Small interference RNA (siRNA) was used to knock down CDC42 in the Lewis lung carcinoma (LLC)1. The invasion capability of CDC42 knockdown LLC1 cells was evaluated. LLC1 cells with CDC42 targeted small hairpin RNA (shRNA) were inoculated into C57BL/6 mice to establish the tumor-bearing animal model Tumor size and metastasis related proteins were measured. In addition, the invasion of macrophages in the tumor site as well as macrophage chemokine were also determined in the model. RESULTS: The capacity of invasion and metastasis of LLC1 cells significantly decreased when CDC42 was knocked down. When inoculated with CDC42 knockdown LLC1 cells in vivo, the tumor size and metastasis related proteins levels both decreased. The invasion capacity of macrophages and the associated macrophage chemokine were also significantly down-regulated. CONCLUSION: Our data suggest that the inhibition of CDC42 expression in lung cancer cells can significantly prevent the pathogenesis and development of tumor in an allograft tumor model in vivo, which might provide a novel therapeutic target and potential strategy for lung cancer treatment in the future.

2.
Cell Death Dis ; 11(10): 902, 2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33097691

RESUMO

HCC (hepatocellular carcinoma) is a major health threat for the Chinese population and has poor prognosis because of strong resistance to chemotherapy in patients. For instance, a considerable challenge for the treatment of HCC is sorafenib resistance. The aberrant glucose metabolism in cancer cells aerobic glycolysis is associated with resistance to chemotherapeutic agents. Drug-resistance cells and tumors were exposed to sorafenib to establish sorafenib-resistance cell lines and tumors. Western blotting and real-time PCR or IHC staining were used to analyze the level of CLCF1 in the sorafenib resistance cell lines or tumors. The aerobic glycolysis was analyzed by ECAR assay. The mechanism mediating the high expression of CLCF1 in sorafenib-resistant cells and its relationships with miR-130-5p was determined by bioinformatic analysis, dual luciferase reporter assays, real-time PCR, and western blotting. The in vivo effect was evaluated by xenografted with nude mice. The relation of CLCF1 and miR-30a-5p was determined in patients' samples. In this study, we report the relationship between sorafenib resistance and increased glycolysis in HCC cells. We also show the vital role of CLCF1 in promoting glycolysis by activating PI3K/AKT signaling and its downstream genes, thus participating in glycolysis in sorafenib-resistant HCC cells. Furthermore, we also show that miR-30a-5p directly targets CLCF1 and that sorafenib-mediated suppression of miR-30a-5p results in the upregulation of CLCF1 in HCC cells resistant to sorafenib. We also found that when a cholesterol modified agomiR-30a-5p was delivered systemically to mice harboring sorafenib-resistant HCC tumors, tumor growth decreased significantly. There is an uncharacterized mechanism of biochemical resistance to hormone therapies orchestrated by the miR-30a-5p/CLCF1 axis to mediate sorafenib resistance and aerobic glycolysis in HCC. Therefore, this study indicates that targeting the miR-30a-5p/CLCF1 axis may hold promise for therapeutic intervention in HCC sorafenib resistance patients.

3.
Artigo em Inglês | MEDLINE | ID: mdl-33081057

RESUMO

Different lines of evidence indicate that knowledge of low-glycemic index (GI) foods and the practice of eating them play important roles in blood glucose management and preventing T2DM in women with prior gestational diabetes mellitus (GDM). According to the theory of planned behavior (TPB), intention is a critical factor in complying with health-related behaviors. However, an instrument for assessing the intention to eat low-GI foods is lacking in China. We aimed to (1) adapt and validate a Chinese version of the intentions to eat low-GI foods questionnaire (CIELQ) and (2) apply the CIELQ among rural Chinese women to explore the associations between CIELQ scores and glycemic status. A cross-sectional study was conducted on 417 nondiabetic, nonpregnant participants with a history of GDM in Hunan, China. After cultural adaptation and validation, the CIELQ was applied in a target population. Glycemic status, anthropometric variables, dietary intake, and physical activity were measured; a self-developed, standard questionnaire was applied to collect relevant information. The CIELQ showed good internal consistency; model fitness was acceptable based on the confirmatory factor analysis results. Awareness of the glycemic index was low among the study population. TPB factors were found to be associated with each other; education level and parents' diabetes history were associated with specific factors. The score for instrumental attitude showed a positive association with the risk for a high level of the 2-h 75-g oral glucose tolerance test (odds ratio, OR = 1.330), while the score for perceived behavior control (PBC) showed a negative association with the risk for a high level (OR = 0.793). The CIELQ was determined to be a valid instrument for assessing the intention to eat a low-GI diet among the study population. The awareness of the GI was poor among the study population. The score for instrumental attitude showed a positive association with the risk of a high level on the 2-h 75-g oral glucose tolerance test (OGTT), and the score for PBC showed a negative association with the risk for a high level on OGTT.

4.
Mycologia ; : 1-12, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33085937

RESUMO

A new genus, Pygmaeomyces, and two new species are described based on phylogenetic analyses and phenotypic and ecological characters. The species delimitation was based on concordance of gene genealogies. The Pygmaeomyces cultures were isolated from the roots of mountain laurel (Kalmia latifolia) and pitch pine (Pinus rigida) from the acidic and oligotrophic New Jersey pygmy pine plains; however, they likely have a broader distribution because their internal transcribed spacer (ITS) sequences have high similarity to a number of environmental sequences from multiple independent studies. Based on the phylogeny and phenotypic characters, a new family, Pygmaeomycetaceae, is proposed to accommodate this new lineage in Mucoromycotina. Pygmaeomycetaceae corresponds to Clade GS23, which was identified based on a sequence-only soil fungal survey and was believed to be a distinct new class. Compared with the culture-based methods, we observed that sequence-only analyses tend to overestimate the taxonomic level. Results from this work will facilitate ecological and evolutionary studies on root-associated fungi.

6.
Eur J Pharmacol ; : 173630, 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33045197

RESUMO

Glucocorticoids are commonly used in clinic, but the immunosuppression seriously hinders their usage. Herein, immunomodulatory effect of artesunate (AS) on hydrocortisone (HC)-induced immunosuppression was investigated. HC-induced immunosuppression mice (HC mice) were established by intramuscular administration with HC (20 mg/kg) once a day for 5 consecutive days. The results showed HC mice challenged with Escherichia coli on the sixth day presented a lower ability to clear bacteria, decreased TNF-α in blood, decreased spleen index and thymus index. Significantly, AS (20 mg/kg) treatment not only enhanced the ability of HC mice to clear bacteria, but also increased spleen index, the levels of pro-inflammatory cytokines from 78.7 ± 12.1 ng/ml (TNF-α) and 48.7 ± 8.6 pg/ml (IL-6) to 174.0 ± 90.5 ng/ml and 783.3 ± 90.5 pg/ml, number of white blood cells in blood, and sIgA in colon. Subsequently, HC-induced immunosuppression peritoneal macrophages model (HC cells) was established via addition of HC (0.5 µg/ml) for 0.5 h, and then LPS (100 ng/ml) was added to clarify the functional status of the cells. The results showed HC inhibited TNF-α and IL-6 mRNA expressions and their release, but AS (2.5 µg/ml) could increase TNF-α and IL-6 mRNA expressions and their release. AS inhibited GILZ mRNA up-regulated by HC and increases TLR4/NF-κB p65 expressions down-regulated by HC. Our findings revealed that AS's effect is closely related to the improvement of the TLR4/NF-κB signal transduction pathway via inhibiting the up-regulation of GILZ mRNA, demonstrating AS does possess immunomodulatory effects and is worth further investigation in the future.

7.
J Hazard Mater ; 404(Pt B): 124062, 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-33068992

RESUMO

In this work, a novel Ag/AgCl@g-C3N4@UIO-66(NH2) heterojunction was constructed for photocatalytic inactivation of Microcystis aeruginosa (M. aeruginosa) under visible light. The photocatalyst was synthesized by a facile method and characterized by XRD, SEM, TEM, BET, XPS, FT-IR, UV-vis DRS, PL and EIS. The nanocomposite can not only provide lots of active sites, but also improve capacities to utilize visible-light energy and effectively transfer charge carriers, thus enhancing removal efficiencies of cyanobacteria (99.9% chlorophyll a was degraded within 180 min). Various factors in photodegradation of chlorophyll a were studied. Besides, changes on cellular morphologies, membrane permeability, physiological activities of M. aeruginosa during photocatalysis were investigated. Moreover, the cycle test indicated that Ag/AgCl@g-C3N4@UIO-66(NH2) exhibits excellent reusability and photocatalytic stability. Finally, a possible mechanism of M. aeruginosa inactivation was proposed. In a word, Ag/AgCl@g-C3N4@UIO-66(NH2) can efficiently inactivate cyanobacteria under visible light, thus providing useful references for further removal of harmful algae in real water bodies.

8.
J Colloid Interface Sci ; 584: 154-163, 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-33069015

RESUMO

Designing atomically defective adsorbents with high specific surface area has emerged as a promising approach to improve sorption properties. Herein, hierarchical porous boron nitride nanosheets with boron vacancies (Bv-BNNSs) were in-situ synthesized via a one-step ZnCl2-assisted strategy. Being benefitted from the dual-functional template of zinc salt, highly-active boron vacancies and abundant hierarchical pores were simultaneously generated in the Bv-BNNSs framework. By employing the boron vacancies engineering strategy, the morphological and electronic structures were controllably tuned. Meanwhile, the specific surface area was improved to as high as 1104 m2/g. Owning to the abundance of accessible surface active-sites, the sorption capacity to antibiotic tetracycline (TC) on Bv-BNNSs was boosted by 38% compared to the pristine boron nitride nanosheets (BNNSs). Detailed fitting results showed that TC sorption on Bv-BNNSs obeyed the pseudo-second order kinetic equation and the Freundlich isotherm model. The pi - pi interaction with a multi-layered stacking form was proposed as the dominated sorption mechanism. Furthermore, DFT calculations verified that the interaction energy between Bv-BNNSs and TC was enhanced. The high activity, excellent selectivity, and remarkable durability of the Bv-BNNSs nanomaterial suggest the great potential in practical wastewater treatment.

9.
Biomed Mater ; 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33065568

RESUMO

The biomechanical characteristics of tendon grafts is essential for tendon reconstructive surgery due to its great role in providing a good mechanical environment for tendon healing and regeneration. In our previous studies, the decellularized tendon slices (DTSs) and decellularized bovine tendon sheets (DBTSs) scaffolds were successfully developed. However, the influence of the integrity of tendinous membrane (endotenon and epitenon) and fascicle on biomechanical characteristics of these two scaffolds were not investigated. In this study, we assessed the integrity of tendinous membrane and fascicle of the tendon derived scaffolds and its effect on the biomechanical characteristics. The results of histological staining indicated that the DBTSs had complete endotenon and epitenon, while DTSs had no epitenon at all, only part of endotenon was remained. Furthermore, the DBTSs, and DTSs with thickness of 900 µm had complete fascicles, while DTSs with thickness less than 600 µm had almost no complete fascicles. The fibrous configuration of epitenon was well-preserved in the surface of the DBTSs but the surface ultrastructure of the DTSs was aligned collagen fibers based on scanning electron microscopy examination. The results of transmission electron microscopy showed that there was no significant difference between the DBTSs and DTSs. Mechanically, the DBTSs and DTSs with thickness of 900 µm showed similar ultimate tensile strength (UTS) and stiffness to native tendon segments (NTSs). The strain at break and suture retention strength (SRS) of the DBTSs showed much higher than that of the DTSs (p < 0.05). Additionally, the DBTSs showed higher ultimate load than the DTSs when these scaffolds were sutured with NTSs (p < 0.05) through the modified Kessler technique based on a uniaxial tensile test. This study demonstrated that DTSs may be used as a patch for reinforcing tendon repair, while DBTSs may be used as a bridge for reconstructing tendon defects.

10.
Psychol Res ; 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-32995910

RESUMO

The mnemonic effect of insight refers to the situation in which experiencing an "aha" moment when solving problems could improve memory performance for both the question and its solution. The aha experience can be triggered either by external stimuli or by internal solution attempts, namely "induced" or "spontaneous" insight, respectively. Tests of the neural correlates of the insightful memory effect are typically conducted in induced insight paradigms. The neural mechanism of the mnemonic effect of spontaneous insight is unclear. In the present study, the mnemonic effect for spontaneous insight was examined by the event-related potentials (ERPs) technique and behavioral measures. Subjects were required to solve a set of Chinese verbal compound remote-associated tasks (CRA), and performed a recognition test 10 min later. The results showed that the spontaneous insight solution elicited a more negative deflection than did the non-insight solution before the button reaction (- 800 to - 400 ms) in the study phase. In the recognition test phase, items which elicited insight during study were recognized faster, compared with non-insight study items. And spontaneous insight solution elicited a more positive deflection than did non-insight solution in the time window from 400 to 700 ms after onset of the answer. Moreover, brain-behavior correlations revealed a relationship between N400 amplitude during study and later memory performance which revealed a double-dissociation between items solved with and without insight during study. The different predictions for recognition indicate that the encoding of spontaneous insight may differ from that of non-insight, suggesting that different encoding mechanisms may mediate the encoding of items and solutions found by insight versus non-insight.

11.
BMC Ophthalmol ; 20(1): 382, 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-32977791

RESUMO

BACKGROUND: Varying degrees of macular ischemia generally occur in diabetic retinopathy (DR). This study aims to evaluate the effect of conbercept with 3+ pro re nata (PRN) on macular perfusion status in patients with diabetic macular edema (DME) and quantitatively assess changes in foveal avascular zone (FAZ) areas and capillary density in macular regions by applying optical coherence tomography angiography (OCTA). METHODS: Fifty patients were divided into ischemic (n = 31) and non-ischemic (n = 19) groups according to the presence of ischemia on OCTA at baseline. All patients received intravitreal injections of 0.5 mg of conbercept with 3+ PRN principle. The FAZ areas and macular vessel density measured using OCTA were evaluated at baseline, 3 months, and 6 months after treatment in both groups. RESULTS: At months 3 and 6, the FAZ area in the ischemic group changed from 0.510 ± 0.171 mm2 to 0.441 ± 0.158 mm2 then to 0.427 ± 0.153 mm2 (p = 0.003, p = 0.296); in the non-ischemic group, it remained stable (p = 0.269, p = 0.926). The superficial vessel density changed from 41.1 ± 4.1 to 42.5% ± 4.7% then to 42.6% ± 4.6% (p = 0.043, p = 0.812), and the deep vessel density changed from 40.7 ± 4.4 to 42.3% ± 3.6% then to 42.3% ± 4.7% (p = 0.072, p = 0.961) in the ischemic group. In the non-ischemic group, the superficial vessel density changed from 44.8 ± 3.2 to 46.0% ± 3.5% then to 45.7% ± 3.3% (p = 0.108, p = 0.666), whereas the deep vessel density changed from 43.6 ± 3.6 to 43.8% ± 3.2% then to 43.5% ± 4.5% (p = 0.882, p = 0.736). Reperfusion in macular nonperfusion areas was observed. CONCLUSION: Anti-vascular endothelial growth factor treatment may have a positive effect on macular perfusion status. Furthermore, OCTA had advantages in quantifying and calculating blood flow index in the study of macular perfusion status.

12.
Psych J ; 2020 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-32964697

RESUMO

In contrast to the conventional reasoning process that relies heavily on cognitive control, an individual's creativity may show an increasing trend when the cognitive control function is suppressed. A typical situation in which this occurs is low cognitive inhibition triggered by strong anger. In studies of the influence of anger on creativity, the creative tasks used were mostly divergent-thinking tasks, and few studies have compared the impact of anger and joy on general thinking and creative thinking directly. In addition to using an Alternative-Uses Task (AUT) that reflects divergent thinking, this study also adopted the matching routine and novel chunk-decomposition tasks to evaluate general and novel problem-solving, respectively. We also focused on the impact of angry and joy moods on creative tasks, comparing them with a control condition of neutral mood. The results revealed that the induction of an anger emotion promotes an individual's divergent thinking more than that of a joy emotion, and that both anger and joy are effective ways for creative problem-solving. Furthermore, anger reduced the reaction time while joy increased the accuracy rate to facilitate the creative problem-solving.

13.
Psych J ; 2020 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-32985116

RESUMO

Although the innovative designs deliver, superior customer value has long been noted. However, the emotional experience of innovative products brings remains mostly unknown. This work focuses on two kinds of innovative products: function innovation (FI) and design innovation (DI), to uncover the mystery of the emotional experience, these two types of innovative designs bring. Participants in Study 1 were required to subjectively report their practical, emotional experience of the two kinds of innovative designs. The result showed that the emotional experience of innovation products might be a mixture of emotionality. Also, FI and DI products may cause different emotional experiences performance. Multidimensional scaling was employed in Study 2 to simplify the dimensions of these reported emotions to investigate the difference of emotion dimensions distribution that FI and DI brings. The results showed that DI products mainly reflected strong arouse than FI in positive emotions. However, DI product brings more negative emotions than positive emotions. Whereas, FI product did not show the quantitative differences between positive and negative emotions. In Study 3, the difference between FI and DI in emotion intensity was investigated, and the result indicates that both FI and DI products can bring high-intensity positive emotion. However, the DI brings higher intensity negative emotion and lower intensity positive emotion than FI. These studies demonstrate the first direct evidence of the essence of the difference between FI and DI emotion experience and suggest possible guidance for consumers to avoid the effect of the emotions when choosing innovative products.

14.
Nat Commun ; 11(1): 4496, 2020 09 08.
Artigo em Inglês | MEDLINE | ID: mdl-32901024

RESUMO

Aging is characterized by the loss of homeostasis and the general decline of physiological functions, accompanied by various degenerative diseases and increased rates of mortality. Aging targeting small molecule screens have been performed many times, however, few have focused on endogenous metabolic intermediates-metabolites. Here, using C. elegans lifespan assays, we conducted a worm metabolite screen and identified an eukaryotes conserved metabolite, myo-inositol (MI), to extend lifespan, increase mobility and reduce fat content. Genetic analysis of enzymes in MI metabolic pathway suggest that MI alleviates aging through its derivative PI(4,5)P2. MI and PI(4,5)P2 are precursors of PI(3,4,5)P3, which is negatively related to longevity. The longevity effect of MI is dependent on the tumor suppressor gene, daf-18 (homologous to mouse Pten), independent of its classical pathway downstream genes, akt or daf-16. Furthermore, we found MI effects on aging and lifespan act through mitophagy regulator PTEN induced kinase-1 (pink-1) and mitophagy. MI's anti-aging effect is also conserved in mouse, indicating a conserved mechanism in mammals.


Assuntos
Envelhecimento/metabolismo , Proteínas de Caenorhabditis elegans/metabolismo , Fatores de Transcrição Forkhead/metabolismo , Inositol/metabolismo , Longevidade/fisiologia , PTEN Fosfo-Hidrolase/metabolismo , Envelhecimento/efeitos dos fármacos , Animais , Animais Geneticamente Modificados , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/genética , Linhagem Celular Tumoral , Feminino , Fatores de Transcrição Forkhead/genética , Inositol/administração & dosagem , Locomoção/fisiologia , Longevidade/efeitos dos fármacos , Redes e Vias Metabólicas/genética , Metabolômica , Camundongos , Mitofagia/fisiologia , Modelos Animais , Fosfatos de Fosfatidilinositol/metabolismo , Proteínas Quinases/metabolismo , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Interferência de RNA , RNA-Seq
15.
Ecotoxicol Environ Saf ; 206: 111209, 2020 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-32891912

RESUMO

In this paper, several experiments were carried out to study the environmental behavior and influencing factors of glyphosate (PMG) in peach orchard ecosystem. The results of field experiments showed that PMG and its metabolite aminomethylphosphonic acid (AMPA) were detected in peach tree leaves and peach tree fruits, although PMG was only sprayed on the soil. The residues of PMG and AMPA in peach tree leaves were ~0.1 mg/kg and ~0.5 mg/kg and in peach tree fruits were ~0.01 mg/kg and 0.07-0.11 mg/kg, respectively. By conducting a series of laboratory simulation experiments, the environmental factors affecting the degradation of PMG were screened and evaluated. The results showed that PMG metabolized much faster in loess soil than red soil and black soil (with the DT50 of 11.6 days, 62.4 days, and 34.1 days, respectively). By analyzing the basic properties of the soil, we investigated the effects of pH, moisture content, organic matter (exogenous biochar) and ambient temperature using orthogonal experiments, and the results were further confirmed by microbial experiment. The results showed that alkaline conditions (pH = 7.8/9), high water content (25%) and microorganisms could promote the degradation of PMG. Sterile soil environment had a negative impact on the metabolic behavior of PMG to AMPA.

16.
Redox Biol ; 36: 101644, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32863210

RESUMO

Microglia-mediated neuroinflammation plays a crucial role in the pathophysiological process of multiple neurological disorders such as ischemic stroke, yet lacks effective therapeutic agents. Previously, we discovered one novel synthetic compound, tanshinol borneol ester (DBZ), possesses anti-inflammatory and anti-atherosclerotic activities, whereas little is known about its effects in CNS. Therefore, the present study aims to explore the effects and potential mechanism of DBZ on neuroinflammation and microglial function. Our studies revealed that DBZ significantly inhibited NF-κB activity, suppressed the production of pro-inflammatory mediators meanwhile promoted M2 mediators expression in LPS-stimulated BV2 cells and mouse primary microglia cells. DBZ also exhibited antioxidant activity by enhancing Nrf2 nuclear accumulation and transcriptional activity, increasing HO-1 and NQO1 expression, and inhibiting LPS-induced ROS generation in BV2 cells. Importantly, the anti-neuroinflammatory and antioxidant effects of DBZ above were reversed by Nrf2 knockdown. Additionally, DBZ ameliorated sickness behaviors of neuroinflammatory mice induced by systemic LPS administration, and significantly reduced infract volume, improved sensorimotor and cognitive function in rats subjected to transient middle cerebral artery occlusion (tMCAO); besides, DBZ restored microglia morphological alterations and shifted the M1/M2 polarization in both murine models. Mechanistically, DBZ-induced Nrf2 nuclear accumulation and antioxidant enzymes expression were accompanied by increased level of p-Akt(Ser473) (activation) and p-GSK3ß(Ser9) (inactivation), and decreased nuclear level of Fyn both in vitro and in vivo. Pharmacologically inhibiting PI3K or activating GSK3ß markedly increased nuclear density of Fyn in microglia cells, which blocked the promoting effect of DBZ on Nrf2 nuclear accumulation and its antioxidant and anti-neuroinflammatory activities. Collectively, these results indicated the effects of DBZ on microglia-mediated neuroinflammation were strongly associated with the nuclear accumulation and stabilization of Nrf2 via the Akt(Ser473)/GSK3ß(Ser9)/Fyn pathway. With anti-neuroinflammatory and antioxidant properties, DBZ could be a promising new drug candidate for prevention and/or treatment of cerebral ischemia and other neuroinflammatory disorders.

17.
Environ Pollut ; 267: 115405, 2020 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-32866865

RESUMO

The ubiquitous occurrence of steroid estrogens (SEs) in the aquatic environment has raised global concern for their potential environmental impacts. This paper extensively compiled and reviewed the available occurrence data of SEs, namely estrone (E1), 17α-estradiol (17α-E2), 17ß-estradiol (17ß-E2), estriol (E3), and 17α-ethinyl estradiol (EE2), based on 145 published articles in different regions all over the world including 51 countries and regions during January 2015-March 2020. The data regarding SEs concentrations and estimated 17ß-estradiol equivalency (EEQ) values are then compared and analyzed in different environmental matrices, including natural water body, drinking and tap water, and wastewater treatment plants (WWTPs) effluent. The detection frequencies of E1, 17ß-E2, and E3 between the ranges of 53%-83% in natural water and WWTPs effluent, and the concentration of SEs varied considerably in different countries and regions. The applicability for EEQ estimation via multiplying relative effect potency (REPi) by chemical analytical data, as well as correlation between EEQbio and EEQcal was also discussed. The risk quotient (RQ) values were on the descending order of EE2 > 17ß-E2 > E1 > 17α-E2 > E3 in the great majority of investigations. Furthermore, E1, 17ß-E2, and EE2 exhibited high or medium risks in water environmental samples via optimized risk quotient (RQf) approach at the continental-scale. This overview provides the latest insights on the global occurrence and ecological impacts of SEs and may act as a supportive tool for future SEs investigation and monitoring.

18.
Sci Adv ; 6(22): eaaz7677, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32766441

RESUMO

A persistent enigma is the rarity of polyploidy in animals, compared to its prevalence in plants. Although animal polyploids are thought to experience deleterious genomic chaos during initial polyploidization and subsequent rediploidization processes, this hypothesis has not been tested. We provide an improved reference-quality de novo genome for allotetraploid goldfish whose origin dates to ~15 million years ago. Comprehensive analyses identify changes in subgenomic evolution from asymmetrical oscillation in goldfish and common carp to diverse stabilization and balanced gene expression during continuous rediploidization. The homoeologs are coexpressed in most pathways, and their expression dominance shifts temporally during embryogenesis. Homoeolog expression correlates negatively with alternation of DNA methylation. The results show that allotetraploid cyprinids have a unique strategy for balancing subgenomic stabilization and diversification. Rediploidization process in these fishes provides intriguing insights into genome evolution and function in allopolyploid vertebrates.

19.
BMC Immunol ; 21(1): 44, 2020 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-32746780

RESUMO

BACKGROUND: Immunophenotyping of blood lymphocytes is an essential tool to evaluate the immune function of patients with immunodeficiency or autoimmunity. Predominately identified CD4+T cell subsets, Th1, Th2, Th17, as well as regulatory T (Treg) cells, play crucial roles in several immunological and pathological conditions. Considering the variations in cell counts among populations and ethnicities, specific CD4+T cell subset reference values need to be locally established to enable meaningful comparisons and accurate data interpretation in clinical and research settings. Therefore, the aim of this study was to establish distributions and reference ranges for blood CD4+T cell subpopulations in age- and sex-balanced healthy adults of a Han Chinese population in Shanxi Province, North China. METHODS: Peripheral blood CD4+T cell subsets were examined in 150 healthy volunteers (75 males, 75 females) aged 20-70 years with a four-color FACSCalibur flow cytometer. RESULTS: Reference value percentages (absolute counts, cells/µl) were defined as 95% of the population for cell types as follows: CD4+T, 23.78-51.07 (360-1127); Th1, 0.43-39.62 (2.64-276.21); Th2, 0.27-3.57 (1.80-27.14); Th17, 0.22-2.62 (1.10-19.54); and Treg, 2.17-7.94 (13.47-64.58). The ranges for the Th1:Th2 and Th17:Treg ratios were 0.59-52.37 and 0.04-0.76, respectively. Notably, a significant increase was observed in the values of Treg cells in older individuals, and the numbers of Treg cells in females also tended to decrease when compared to those in males. Therefore, we established the distribution and reference range of CD4+T cell subsets based on age and sex, demonstrating the lowest values of Treg cells in younger females. CONCLUSIONS: Collectively, our data provide population-, age-, and sex-specific distributions and reference ranges of circulating CD4+T cell subpopulations, which can be adopted to guide clinical decisions and interpretation of immunophenotyping data in the Han Chinese population in Taiyuan, Shanxi Province, China. In addition, the low expression of peripheral Treg cells in younger females may be associated with the predisposition of females to autoimmune diseases.

20.
Onco Targets Ther ; 13: 6795-6803, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32764967

RESUMO

Purpose: MORC4 has recently been characterized as a breast cancer-associated anti-apoptotic oncoprotein. In the current study, we explored its downstream regulation in luminal A/B breast tumors. Materials and Methods: Bioinformatic prediction was performed using data from The Cancer Genome Atlas (TCGA)-breast cancer (BRCA). Cellular and molecular studies were conducted using luminal A/B representative MCF-7 and BT-474 cell lines. Results: ENST00000355610.8 (encoding MORC4a isoform) was the dominant transcript in breast cancer. ChIP-qPCR and dual-luciferase assay confirmed two STAT3-binding sites in the MID2 promoter in both MCF-7 and BT-474 cells. Co-IP confirmed an interaction between MORC4 and STAT3. ChIP-qPCR data indicated that MORC4 inhibition led to remarkably decreased enrichment of the STAT3-binding MID2 promoter segments. MORC4 overexpression significantly elevated BCL-2 expression in MCF-7 cells and increased their resistance to adriamycin (ADM), 5-fluorouracil (5-FU), and cisplatin (DDP). MID2 inhibition largely abrogated MORC4-induced drug-resistance. However, the drug-resistant phenotype was rescued by overexpressing MID2-MT that was resistant to MID2 siRNA. Conclusion: This study revealed a novel regulatory mechanism of MORC4 on MID2 expression via STAT3-mediated transcriptional activation. This regulatory axis might confer increased chemoresistance to breast cancer cells.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA