Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 817
Filtrar
Filtros adicionais











Intervalo de ano
1.
Artigo em Inglês | MEDLINE | ID: mdl-31476261

RESUMO

To promote the development of solid-state batteries, polymer, oxide, and sulfide-based solid-state electrolytes (SSEs) have been extensively investigated. However, the disadvantages of these SSEs, such as high-temperature sintering of oxides, air instability of sulfides, and narrow electrochemical windows of polymers electrolytes, significantly hinder their practical application. Therefore, developing SSEs that spontaneously possesses high ionic conductivity (>10-3 S cm-1 ), good-air stability, wide electrochemical window, excellent electrode interface stability, low-cost mass production is highly demanded. Here we report a halide Li+ superionic conductor, Li3InCl6 that can be synthesized in water. Most importantly, the as-synthesized Li3InCl6 shows a high ionic conductivity of 2.04×10-3 S cm-1 at 25 °C. Furthermore, the ionic conductivity can be totally recovered after dissolved in water. Combining with LiNi0.8Co0.1Mn0.1O2 cathode, solid-state lithium batteries shows astonishing cycling stability. The H2O-mediated synthesis of Li3InCl6 can significantly promote the advances of all-solid-state lithium batteries.

2.
J Sci Food Agric ; 2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31471909

RESUMO

BACKGROUND: Removing excessive naturally occurring fluoride from tea and/or infusions is difficult because the process has low efficiency and causes secondary pollution. In this study, a novel electrodialysis (ED) technology was developed. We examined the effect of crucial parameters (electrolyte concentration, operation voltage, ED duration, and initial concentration of the tea infusion) on the defluoridation performance by using a highly efficient ion exchange membrane with five-compartment cells. RESULTS: The most effective ED system results were obtained at an electrolyte concentration of 10 g•kg-1 and operation voltage of 20 V. Moreover, the fluoride removal capacity (10.70%-66.93%) was highly dependent on the ED duration (1-15 min) and initial concentration of the tea infusion (0.5 g•kg-1 -10 g•kg-1 ). The longer the ED duration and lower the initial concentration, the higher was the defluoridation performance. During ED, limited loss of the main inclusions (total polyphenols, catechins, caffeine, and selected ions) was observed. Furthermore, the D201 anion resin-filled ED stack (0.5 g - 5 g) and improvement of concentrate compartment electrolyte (≥5 times of dilute compartment electrolyte) in the ED system enhanced the defluoridation rate significantly. CONCLUSION: ED is a potentially effective method that can be used for defluoridation in the deep-processing of tea products. This article is protected by copyright. All rights reserved.

3.
Ultrason Sonochem ; 59: 104699, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31476699

RESUMO

Cavitation is a very important hydrodynamic phenomenon in many scientific and engineering fields, such as acoustics, medicine, and hydraulics. The relationship between the physical characteristics of liquid media and the erosion status of materials in a cavitation field is a crucial concern for many researchers. In this study, we adopted underwater low-voltage discharge technology to generate cavitation bubbles. We studied the effect of the viscosity of liquid media on cavitation bubble dynamics at the mesoscale level by using high-speed photography and a transient stress test system to illustrate the mechanism and the characteristics of material erosion under viscosity changes at the macroscopic level. It was found that high liquid viscosity delays shrinkage of the cavitation bubble and increases the minimum volume to which the cavitation bubble shrinks. The shrinkage characteristics of a cavitation bubble in a solution with high viscosity can reduce the speed of micro-jet formation during the collapse of the cavitation bubble near a wall. In addition, they can delay the impact of the micro-jet on the wall surface and reduce the impact strength when the cavitation bubble collapses. The effect of viscosity on cavitation bubble dynamics at the mesoscale level can explain the erosion law of solid-wall surfaces in a cavitation field from a mechanical point of view. These conclusions present significant potential for cavitation application and prevention in the fields of ultrasonic cleaning, medicine, and hydraulic machinery.

4.
Breast Cancer Res ; 21(1): 102, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31484577

RESUMO

BACKGROUND: Diffusion-weighted imaging (DWI) can increase breast MRI diagnostic specificity due to the tendency of malignancies to restrict diffusion. Diffusion tensor imaging (DTI) provides further information over conventional DWI regarding diffusion directionality and anisotropy. Our study evaluates DTI features of suspicious breast lesions detected on MRI to determine the added diagnostic value of DTI for breast imaging. METHODS: With IRB approval, we prospectively enrolled patients over a 3-year period who had suspicious (BI-RADS category 4 or 5) MRI-detected breast lesions with histopathological results. Patients underwent multiparametric 3 T MRI with dynamic contrast-enhanced (DCE) and DTI sequences. Clinical factors (age, menopausal status, breast density, clinical indication, background parenchymal enhancement) and DCE-MRI lesion parameters (size, type, presence of washout, BI-RADS category) were recorded prospectively by interpreting radiologists. DTI parameters (apparent diffusion coefficient [ADC], fractional anisotropy [FA], axial diffusivity [λ1], radial diffusivity [(λ2 + λ3)/2], and empirical difference [λ1 - λ3]) were measured retrospectively. Generalized estimating equations (GEE) and least absolute shrinkage and selection operator (LASSO) methods were used for univariate and multivariate logistic regression, respectively. Diagnostic performance was internally validated using the area under the curve (AUC) with bootstrap adjustment. RESULTS: The study included 238 suspicious breast lesions (95 malignant, 143 benign) in 194 women. In univariate analysis, lower ADC, axial diffusivity, and radial diffusivity were associated with malignancy (OR = 0.37-0.42 per 1-SD increase, p < 0.001 for each), as was higher FA (OR = 1.45, p = 0.007). In multivariate analysis, LASSO selected only ADC (OR = 0.41) as a predictor for a DTI-only model, while both ADC (OR = 0.41) and FA (OR = 0.88) were selected for a model combining clinical and imaging parameters. Post-hoc analysis revealed varying association of FA with malignancy depending on the lesion type. The combined model (AUC = 0.81) had a significantly better performance than Clinical/DCE-MRI-only (AUC = 0.76, p < 0.001) and DTI-only (AUC = 0.75, p = 0.002) models. CONCLUSIONS: DTI significantly improves diagnostic performance in multivariate modeling. ADC is the most important diffusion parameter for distinguishing benign and malignant breast lesions, while anisotropy measures may help further characterize tumor microstructure and microenvironment.

5.
Chin J Integr Med ; 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31385219

RESUMO

OBJECTIVE: To summarize the evidence from systematic reviews (SRs) on the benefits and safety of Tripterygium glycosides (TG) and total glucosides of paeony (TGP), commonly used to treat rheumatoid arthritis (RA) in China, for patients with RA. METHODS: SRs of randomized controlled trials (RCTs) on TG or TGP in treating RA were included, by searching 8 databases from their inception until December 2017. Two authors extracted data independently. We assessed the quality of SRs using AMSTAR and graded the quality of evidence according to the GRADE approach. RESULTS: Eleven SRs containing an average of 7.6 RCTs, involving a total of 7,012 participants were included in this overview. On the basis of included SRs, TG and TGP could improve the following indexes for RA patients: American College of Rheumatology (ACR) 20 response rate, ACR50 response rate and ACR70 response rate, swollen joint count, tender joint count, erythrocyte sedimentation rate and C-reactive protein. Moreover, TGP could reduce incidence of hepatotoxicity. The most common adverse effects of TG were gastrointestinal discomfort and gonad toxicity, while for TGP was mild to moderate diarrhea. The overall quality of evidence for these findings ranged from "low" to "moderate". CONCLUSIONS: TG and TGP might be 2 potentially effective complementary and alternative drugs for patients with RA. Nevertheless, due to gonad toxicity, TG should only be considered in elderly patients or patients without reproductive needs. More evidence from high quality RCTs and SRs is warranted to support the use of TG and TGP for RA patients.

6.
Clin Exp Rheumatol ; 2019 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-31376257

RESUMO

OBJECTIVES: P-glycoprotein (P-gp) mediated drug efflux is the most essential mechanism of multi-drug resistance (MDR) in rheumatoid arthritis (RA). The study was undertaken to clarify the mechanism whereby IL-17 regulate the P-gp efflux function in peripheral blood lymphocytes of patients with RA. METHODS: Lymphocytes from RA patients and healthy individuals were cultured with IL-17A (0, 10, 100 ng/ml), IL-17A+(5Z)-7-Oxozeaenol (TAK1 inhibitor), and IL-17A+PD98059 (ERK inhibitor), respectively. 24h later, the level of P-gp mRNA expression in peripheral blood lymphocytes was detected by RT-PCR. Meanwhile, the efflux potential of P-gp was assessed by flow cytometry using the fluorescent dye Rhodamine 123, a substrate of P-gp. In order to confirm whether the inhibitors had worked, ERK1/2 and p65, as well as their phosphorylation were detected utilising Western blot analysis. RESULTS: With the exception of the expression of P-gp mRNA between control and IL-17A group, the mRNA expression, as well as the function of P-gp in the different group of healthy individuals was similar, and there was no significant difference (p>0.05). However, as for the RA patients, increased expressions of P-gp mRNA and efflux function were detected in IL-17A group compared with control. Moreover, IL-17A upregulated mRNA level and function of P-gp in a concentrate dependent manner. Upregulated expression of P-gp mRNA and efflux potential of P-gp were inhibited by TAK1 or ERK inhibitors in RA peripheral blood lymphocytes. Among them, TAK1 inhibitor, (5Z) -7-Oxozeaenol, showed a significant difference (p<0.05). Also, the decreased phosphorylation levels of ERK1/2 and p65 were detected with PD98059 and (5Z) -7-Oxozeaenol addition, respectively. CONCLUSIONS: This study showed that inflammatory cytokines IL-17A can upregulate the mRNA expression level and drug efflux function of P-gp on lymphocytes in RA patients through TAK1, in a concentrate dependent manner, contributing to RA drug resistance. Therefore, this may represent a new target for improving the therapeutic reactivity of DMARDs in the long term for RA patients.

7.
BMJ Open ; 9(8): e026729, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31439595

RESUMO

OBJECTIVE: To adapt the Child Food Neophobia Scale (CFNS) cross-culturally for use among 12-36-month-old Chinese toddlers and to perform a preliminary assessment of its construct validity and reliability. BACKGROUND: Food neophobia is the fear of eating new or unfamiliar foods, which affects the type and quality of individual dietary intake, especially during early childhood. However, measurements of child food neophobia have rarely been reported in China due to a lack of reliable and valid measurements. METHODS: The CFNS was translated and adapted into a Chinese version (CFNS-CN) through a forward translation, reconciliation, a back translation, expert review and pretesting. The construct validity and reliability of the CFNS-CN were tested in 390 caregivers of 12-36 months old Chinese toddlers through convenience sampling in Changsha Maternal and Child Health Care Hospital, Hunan Province, China. The internal consistency, confirmatory factor analysis (CFA) and reliability were estimated. RESULTS: The kappa coefficients indicated moderate to perfect agreement between the test and retest, and Cronbach's α coefficient was 0.91. A normal χ2 /df, CMIN/DF=3.302, Comparative Fit Index, CFI=0.993, Tucker-Lewis Index, TLI=0.986 and root mean square error of approximation, RMSEA=0.077 were found. The CFA results showed that the model indicators were acceptable. High food neophobia was observed in 25.1% of individuals. CONCLUSION: The CFNS-CN showed good internal consistency reliability and construct validity. The CFNS-CN may become an effective tool for assessing food neophobia in Chinese toddlers. TRIAL REGISTRATION: This trial was pre-registered at the China Clinical Trial Registration Center under registration number ChiCTR1800015890.

8.
ChemSusChem ; 2019 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-31407510

RESUMO

Mesoporous bismuth nanosheets are achieved via electrochemical transformation from (100)-facet exposed BiOI. Theoretical modelling and calculations simulate the in situ morphological variation of BiOI-to-Bi. Mesoporous Bi nanosheets show superior electrochemical CO2 reduction performance which can rapidly reach a faradaic efficiency for formic acid (HCOOH) of 95.9% at -0.77 VRHE, compared to smooth Bi nanosheets with maximum faradaic efficiency of 93.8% at -0.87 VRHE. In conjunction with Tafel analysis and DFT results, the electrochemical CO2 reduction on mesoporous Bi nanosheets is kinetically faster twith more resistance to H2 generation than that of smooth Bi-001 nanosheets. The CO2-to-HCOOH pathway for on (012) and (001) planes of Bi is both preferred via *OCHO intermediate. The mesoporous structure induced more accessible interaction with CO2 makes predominant contributions to the enhanced performance rather than subsequent CO2 activation on different facets of Bi.

9.
Transl Oncol ; 12(11): 1504-1515, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31450126

RESUMO

BACKGROUND: DZIP1 is an oncogene involved in the progression and stemness of carcinoma through the Wnt/ß-catenin pathway, and the potential mechanism of DZIP1 in oral squamous cancer remains unknown. The aim of this study was to uncover the effect and mechanism of DZIP1 in the progression of oral squamous carcinoma. METHODS: TCGA database scanning was applied to verify dysregulated genes in oral squamous carcinoma. quantitative real-time polymerase chain reaction, immunohistochemistry, and Western blotting assays were used to detect the expression of DZIP1 in tissues and cell lines. We established stable DZIP1-overexpressing and DZIP1 knockdown cell lines. We investigated the biological function and the underlying mechanism of DZIP1 through a series of experiments. RESULTS: DZIP1 was one of the genes discovered by the scanning strategy to be upregulated in cancer tissue and negatively correlated with the overall survival (OS) of patients. DZIP1 promotes proliferation, migration, and invasion in an oral squamous carcinoma cell line through EMT in a GLI1/3-dependent manner. CONCLUSIONS: DZIP1 promotes the proliferation, migration, and invasion of oral squamous carcinoma through the GLI1/3 pathway.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31304561

RESUMO

Apple pomace, an abundant accessible source of carbohydrate platform chemicals, is refractory to cellulase degradation because of the main barrier problem of pectin constitute. A rapid and portable method for the coproduction of pectin and fermentable sugars was developed using the pretreatment of acetic acid, followed by enzymatic hydrolysis. Compared with pectinase, acetic acid pretreatment provided the highest pectin yield of 19.1% and the highest enzymatic hydrolysis yield from apple pomace. The acidic pretreated apple pomace cellulose was easily and completely hydrolyzed into fermentable sugars. More than 98.2% conversion of cellulose was achieved in a batch hydrolysis using a cellulase loading of 25 FPU/g cellulose and 10% total solids without any special strategies. A mass balance analysis showed that 95.5 g pectin and 110.2 g fermentable sugars were produced from 500-g oven-dried apple pomace. The integrated process is suggestive of environment-friendly and recyclable methods for the industrial utilization of apple pomace.

11.
Am J Infect Control ; 2019 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-31296347

RESUMO

BACKGROUND: The association of operative time and surgical site infection (SSI) in neurosurgery patients is unclear. We therefore, through a systematic review and meta-analysis, sought to clarify the association. METHODS: We performed a systematic search of the PubMed and Embase databases, from January 1, 1966, to December 26, 2018, for published articles. We used random effects generalized least squares regression models to combine study-specific relative risks (RR) and 95% confidence intervals (CI), and performed a sensitivity analysis by excluding 1 study at a time to assess the stability of results and potential sources of heterogeneity. We used the Egger (from plotting to test) to assess publication bias. RESULTS: A total of 5 articles, with 231,915 individuals and 6,726 SSI cases, were included. The summary RR for SSI after craniotomy were 1.67 (95% CI, 1.13-2.20), with high versus low operative time, and 1.34 (95% CI, 1.15-1.53), with each 1-hour increase in operative time. On sensitivity analysis, no individual study had an excessive influence on the pooled effect. We found no evidence of publication bias by the Egger test (P = .464) and Egger's plotting. CONCLUSIONS: Prolonged operative time can increase the SSI risk for neurosurgery patients. Intensive interventions should be taken to decrease operative duration.

12.
BMC Ophthalmol ; 19(1): 150, 2019 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-31311513

RESUMO

BACKGROUND: Morning glory syndrome is a relatively rare congenital optic disc anomaly that is often difficult to identify when associated with additional congenital ocular anomalies. This case report describes the diagnosis, treatment, and postoperative follow-up care of a young girl with morning glory syndrome accompanied by persistent fetal vasculature, retinal fold, and retinal detachment. Here, we also give a brief review of the relevant literature. CASE PRESENTATION: A 5-year-old girl was referred to our clinic for a complaint of decreased vision for 6 months in the right eye. The best corrected visual acuity was hand motion in her right eye and 0.8 in her left eye. A fundus examination indicated vitreous opacities and scattered hemorrhages, as well as striped folds in the temporal retina of the affected eye. B-ultrasound and magnetic resonance imaging scans suggested that it could be a congenital dysplasia of the right eye. Pars plana vitrectomy was performed in the right eye. Morning glory syndrome associated with persistent fetal vasculature was confirmed in subsequent follow-up observation according to the fundus appearance, optical coherence tomography, and fundus fluorescein angiography imaging. CONCLUSIONS: The patient was diagnosed as morning glory syndrome associated with persistent fetal vasculature and retinal fold. The morning glory disc with the presence of retinal folds did not seem quite typical and that made the diagnosis difficult. This report stresses the importance of considering concurrent morning glory syndrome and persistent fetal vasculature. Vitrectomy may be beneficial in the management of the morning glory syndrome and persistent fetal vasculature if accompanied by retinal detachment in similar cases.


Assuntos
Disco Óptico/anormalidades , Nervo Óptico/anormalidades , Vítreo Primário Hiperplásico Persistente , Descolamento Retiniano , Pré-Escolar , Feminino , Humanos , Vítreo Primário Hiperplásico Persistente/diagnóstico , Vítreo Primário Hiperplásico Persistente/cirurgia , Descolamento Retiniano/diagnóstico , Descolamento Retiniano/cirurgia , Resultado do Tratamento , Vitrectomia/métodos
13.
Mol Immunol ; 114: 72-80, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31344551

RESUMO

Scleroderma is an inflammatory autoimmune disease characterized by extensive tissue fibrosis. The imbalance of effector T (Teff) and regulatory T (Treg) cells and the production of autoantibodies contribute to the pathogenesis of this disease. Metformin (MET) has anti-inflammatory and anti-fibrotic effects, but its effect on the in vivo pathogenesis of scleroderma remains unknown. Therefore, we investigated the potential therapeutic effects of MET treatment of mice with bleomycin (BLM)-induced scleroderma. Scleroderma was induced in female C57BL mice by daily subcutaneous injections of BLM for 28 days. After each 2 h BLM injection, mice received MET (200, 100 or 50 mg/kg) or saline (control) by intraperitoneal injection. At the end of the fourth week, spleen mononuclear cells were collected for flow cytometry analysis. Skin samples were harvested for immunohistochemistry and quantification of other biological parameters.Our results showed that BLM increased dermal thickness, collagen deposition, and hydroxyproline level, and MET markedly mitigated these effects. MET also restored the Treg/Teff cell balance. Accordingly, the level of IL-17A and RORγt (related to Th17 cells) decreased, but Foxp3 (related to Treg function) increased in a dose-dependent manner. In addition, MET treatment inhibited spleen germinal center formation. These results indicate that the immunomodulatory and anti-fibrosis effects of MET on BLM-induced scleroderma are mediated by the upregulation of Treg cell differentiation, inhibition of Teff cell differentiation, and suppression of spleen germinal center formation. These results suggest that MET may be a potential therapeutic for scleroderma.

14.
Int Immunopharmacol ; 75: 105769, 2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31351365

RESUMO

Naringenin (NG), a flavanone extracted from various plants, has potent vasoprotective effects likely related to the induction of heme oxygenase-1 (HO-1). In the current study, we investigated mechanisms underlying the effect of NG on HO-1 expression and high glucose (HG)- or free fatty acids (FFA)-induced apoptosis in human umbilical vein endothelial cells (HUVECs). First, we found that HUVECs exposed to NG exhibited enhanced HO-1 expression in a concentration- and time-dependent manner. Moreover, HUVECs treated with NG exhibited activation of phosphoinositide 3 kinase (PI3K)/Akt, extracellular-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK). LY294002 (a PI3K inhibitor) and SP600125 (a JNK inhibitor) reduced NG-induced HO-1 expression, whereas BIRB796 (a p38 inhibitor) and PD98059 (an ERK inhibitor) had no effect. The cytoprotective effects of NG were correlated with activation of the transcription factor NF-E2-related factor 2 (Nrf2), a critical regulator of HO-1 expression. Indeed, the results of our experiments using LY294002 and SP600125 indicated that NG may stimulate Nrf2 through PI3K/Akt and JNK pathway activation. Moreover, treatment of HUVECs with Nrf2 siRNA decreased NG-induced HO-1 expression. Finally, pretreatment of HUVECs with NG remarkably reduced HG- or FFA-induced cell apoptosis, and this effect was greatly abrogated in the presence of SnPP (an HO-1 inhibitor). Above all, our data show that NG increased HO-1 expression and reduced HG- or FFA-induced cell apoptosis in HUVECs by upregulating PI3K, JNK, and Nrf2 pathways, which may confer an adaptive survival response in diabetes-induced vascular injury.

15.
ACS Nano ; 13(7): 8374-8381, 2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31283177

RESUMO

Mimicking the structures and functions of biological systems is considered as a promising approach to construct artificial materials, which have great potential in energy, the environment, and health. Here, we demonstrate a conceptually distinct design by synergistically combining a kidney-inspired porous membrane and natural sponge-inspired heterostructured nanoporous particles to fabricate a bioinspired biomolecule cleaning device, achieving highly efficient biomolecule cleaning spanning from small molecules to macromolecules. The bioinspired biomolecule cleaning device is a two-layer microfluidic device that integrates a polyamide porous membrane and heterostructured nanoporous poly(acrylic acid)-poly(styrene divinylbenzene) particles. The former as a filtration membrane isolates the upper sample liquid and the latter fixed onto the bottom of the underlying channel acts as an active sorbent, particularly enhancing the clearance of macromolecules. As a proof-of-concept, we demonstrate that typical molecules, including urea, creatinine, lysozyme, and ß2-microglobulin, can be efficiently cleaned from simulant liquid and even whole blood. This study provides a method to fabricate a bioinspired biomolecule cleaning device for highly efficient biomolecule cleaning. We believe that our bioinspired synergistic design may expand to other fields for the fabrication of integrated functional devices, creating opportunities in a wide variety of applications.

16.
Med Sci Monit ; 25: 4992-4998, 2019 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-31278248

RESUMO

BACKGROUND This study assessed the clinical characteristics of neonatal acute respiratory distress syndrome (ARDS) and differences in therapy in comparison to RDS. MATERIAL AND METHODS The clinical data of 925 preterm infants with respiratory distress were collected and divided into 4 groups. Group A and B both met the diagnosis of neonatal RDS, whereas infants in group B also showed inflammatory response. Group C met the Montreux definition of neonatal ARDS and group D was the control. RESULTS We found that 73.50% of the 925 preterm infants were diagnosed with RDS, of which RDS with inflammatory response accounted for 42.05%. ARDS accounted for 5.29% and control group accounted for 21.19%. Group C infants were the heaviest (2168.16±654.43 g) and had the oldest gestational age. The pregnancy-induced hypertension was highest (30.07%) in group B and lowest in group D (13.26%). Group C had higher iNO and longer invasive ventilator times, but had less frequent surfactant treatment, as well as shorter oxygen time and hospital stay. Group B had significantly longer invasive ventilator use than in Group A. In group A, PDA, ROP, and PPHN were the most common complications, with morbidity rates at 78.35%, 8.4%, and 25.77%, respectively, while group C had higher incidence of PDA (71.42%) and coagulation disorders (38.77%). CONCLUSIONS ARDS mainly occurs in late preterm infants. Its treatment is dependent on iNO and invasive ventilator-assisted therapy, and the surfactant treatment rate was relatively lower in comparison to RDS. RDS accompanied with inflammatory response is also dependent on prolonged use of an invasive ventilator.

17.
Food Chem Toxicol ; 132: 110658, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31299295

RESUMO

This study was conducted to determine the effect of T-2 toxin on the transcriptome of the glandular stomach in chicks using RNA-sequencing (RNA-Seq). Four groups of 1-day-old Cobb male broilers (n = 4 cages/group, 6 chicks/cage) were fed a corn-soybean-based diet (control) and control supplemented with T-2 toxin at 1.0, 3.0, and 6.0 mg/kg, respectively, for 2 weeks. The histological results showed that dietary supplementation of T-2 toxin at 3.0 and 6.0 mg/kg induced glandular gastric injury including serious inflammation, increased inflammatory cells, mucosal edema, and necrosis and desquamation of the epithelial cells in the glandular stomach of chicks. RNA-Seq analysis revealed that there were 671, 1393, and 1394 genes displayed ≥2 (P < 0.05) differential expression in the dietary supplemental T-2 toxin at 1.0, 3.0, and 6.0 mg/kg, respectively, compared with the control group. Notably, 204 differently expressed genes had shared similar changes among these three doses of T-2 toxin. GO and KEGG pathway analysis results showed that many genes involved in oxidation-reduction process, inflammation, wound healing/bleeding, and apoptosis/carcinogenesis were affected by T-2 toxin exposure. In conclusion, this study systematically elucidated toxic mechanisms of T-2 toxin on the glandular stomach, which might provide novel ideas to prevent adverse effects of T-2 toxin in chicks.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31237938

RESUMO

OBJECTIVES: Pulmonary sequestration is a rare congenital pulmonary malformation. The aim of this study was to explore the effect of different therapeutic strategies on the clinical outcome of asymptomatic intralobar pulmonary sequestration. METHODS: We retrospectively reviewed the clinical data of 37 patients diagnosed with intralobar sequestration. All the patients were asymptomatic. Seventeen patients underwent video-assisted thoracoscopic surgery (VATS) once diagnosed and 20 patients chose to undergo observation. Of these 20 patients, 16 patients developed symptoms during the observation period and also underwent VATS; 4 patients never showed symptoms and did not have surgery. The 33 patients who had VATS were divided into 2 groups: group 1, patients who underwent VATS once diagnosed; group 2, patients who underwent VATS once symptoms appeared. Postoperative data and respiratory function data were compared between the 2 groups. RESULTS: Twenty of the patients were men and 17 were women (mean age 37.05 ± 7.89 years). Results of a comparative analysis of the 2 groups indicated that patients in group 1 had better values for median estimated blood loss, median duration of chest tube insertion, postoperative hospital stay and postoperative hospital stay than those in group 2. Postoperative complications were reported in 1 patient in group 1 and in 3 patients in group 2. Meanwhile, the loss of lung function between group 1 and group 2 was statistically significant, which also suggested that patients benefited from surgery once diagnosed. CONCLUSIONS: For asymptomatic intralobar sequestration, VATS could be effective and safe. The surgical intervention should be performed once the condition is diagnosed to avoid manifestations occurring and to preserve patients' quality of life.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31225694

RESUMO

Fibrin glue has been widely used as a surgical sealing and hemostatic agent. Its application is restricted due to poor tissue adhesion and low mechanical strength. To develop better tissue sealant and hemostatic agent, this study prepared the injectable hydrogels by chemically cross-linking gelatin (G) with or without hyaluronic acid (HA) in situ at a mild condition. The rheological analysis, Fourier transform infrared spectroscopy, swelling, proteolytic degradation, biocompatibility, tissue sealing, and hemostatic ability of the hydrogels were investigated. It was found that the chemical cross-linking rapidly formed in both self-crosslinking gelatin (sc-G) and gelatin/hyaluronate acid (G/HA) hydrogels. The hydrogels could be degraded by trypsin and had a desirable biocompatibility. The tissue sealing ability of the hydrogels was superior to fibrin glue. Furthermore, the G/HA hydrogel had similar hemostatic performance as fibrin glue, and was better than that of gelatin hydrogel. The results in the study indicated that the G/HA hydrogel could be used in clinic as a tissue sealant or surgical hemostat.

20.
Afr Health Sci ; 19(1): 1402-1410, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31148967

RESUMO

Background: Avian influence virus H5N1 causes serious public health concern with significant morbidity and mortality from poultry to humans. Interferon-induced transmembrane (IFITM) proteins usually protect cells from many virus infections by viral entry and replication. Objectives: The purpose of this study was to investigate whether H5N1 viral proteins involved in regulation IFITM1, IFITM2, and IFITM3 following H5N1 infection. Methods: NS1, M1, NP, PB2, HA and NA genes of H5N1 virus were generated by PCR and cloned into pcDNA3.1/myc-His (+) A vector for genes over-expression experiments. Gene expression levels was performed using Real-time PCR. Results: Research displayed that NS1, M1, NP, and PB2 proteins of H5N1 virus increased IFITM1, IFITM2, and IFITM3 expression in A549 cells, only IFITM1 was upregulated by M1 in HEK293T cells. However, our study did not find that HA and NA of H5N1 virus affected IFITM genes family or interferon genes expression. Conclusion: Taken together, our data suggested that IFITM1, IFITM2, and IFITM3 might be directly upregulated via NS1, M1, NP, and PB2 proteins during H5N1 avian influenza virus infection. This study provided new insights into the influence of NS1 and NP proteins on regulation of IFITM1, IFITM2, and IFITM3 expression following H5N1 infection.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA