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Int Angiol ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32052947


BACKGROUND: Insomnia may affect vascular factors and promote arteriosclerosis. Microparticles (MPs) are a heterogeneous group of bioactive small vesicles that can be found in blood and body fluids following activation, necrosis or apoptosis of virtually any eukaryotic cells. MPs are believed to participate in the pathogenesis of atherosclerosis. Few studies have been concerned with the microparticle level in patients with sleep disorder. The purpose of the present study is to measure the levels of endothelial microparticles (EMPs), platelet microparticles (PMPs) and leukocyte-derived microparticles (LMPs) in middle-aged and elderly patients with or without insomnia. METHODS: Patients with insomnia (n=30) and without insomnia (n=18) were enrolled. The insomnia group covered patients with chronic insomnia (n=16) and acute insomnia (n=14). Levels of EMPs (CD31 +, CD62E +) and PMPs (CD41a +, CD42a +) and granulocyte-derived (CD11a +) MPs were measured. Flow cytometry was performed on the Beckman Coulter analyzer. Reference gate was defined for the level of MPs using 0.22-0.45-0.88µm microspheres, and the size gate for MPs was 0.5-1.0µm. RESULTS: Of all types of MPs detected, the levels of CD31 +MPs, CD62E +MPs and CD11a +MPs were significantly higher in the insomnia group than in the non-insomnia group (P<0.05). Besides, compared with acute insomnia, the levels of CD31 + MPs and CD11a +MPs were significantly higher in chronic insomnia (P<0.001). CONCLUSIONS: In insomnia patients, atherosclerosis progression may be increased by the CD31+ EMPs-mediated apoptosis and endothelial injury. The level of CD11a+ LMPs kept increasing as insomnia persisted, which may indicate atherosclerosis progression.

Zhongguo Dang Dai Er Ke Za Zhi ; 15(12): 1086-8, 2013 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-24342202


OBJECTIVE: To evaluate the clinical application value of throat swab nested PCR for detecting active congenital human cytomegalovirus (HCMV) infection in neonates. METHODS: The throat swabs and umbilical cord blood specimens from 51 neonates were collected for nested PCR assay for HCMV glycoprotein B (gB) gene. Moreover, 18 of them were subjected to a pp65 antigen test. RESULTS: The sensitivity and specificity of throat swab nested PCR for HCMV gB gene were 67% and 75%, respectively, and the positive and negative predictive values were 57% and 82%, respectively. CONCLUSIONS: Throat swab nested PCR assay for HCMV gB gene is non-invasive, rapid, and highly sensitive for HCMV detection and holds promise as an excellent screening technology for detecting active congenital HCMV infection in neonates.

Infecções por Citomegalovirus/congênito , Infecções por Citomegalovirus/diagnóstico , Reação em Cadeia da Polimerase/métodos , Sangue Fetal/virologia , Humanos , Recém-Nascido , Fosfoproteínas/sangue , Proteínas do Envelope Viral/genética , Proteínas da Matriz Viral/sangue